List of works - Roberto Contestabile

A Bioinformatics Analysis Reveals a Group of MocR Bacterial Transcriptional Regulators Linked to a Family of Genes Coding for Membrane Proteins

scientific article published on 30 June 2016

A Comprehensive Computational Analysis of Mycobacterium Genomes Pinpoints the Genes Co-occurring with YczE, a Membrane Protein Coding Gene Under the Putative Control of a MocR, and Predicts its Function.

scientific article

A pyrazolopyran derivative preferentially inhibits the activity of human cytosolic serine hydroxymethyltransferase and induces cell death in lung cancer cells.

scientific article

Alanine racemase from Tolypocladium inflatum: A key PLP-dependent enzyme in cyclosporin biosynthesis and a model of catalytic promiscuity

scientific article (publication date: 2013)

Allosteric feedback inhibition of pyridoxine 5'-phosphate oxidase from Escherichia coli

scientific article published on 04 September 2019

An Engineered Escherichia coli Strain with Synthetic Metabolism for In-cell Production of Translationally Active Methionine Derivatives

scientific article published on 31 July 2020

Arabidopsis thaliana serine hydroxymethyltransferases: functions, structures, and perspectives

scientific article published in 2022

Asymmetry of the active site loop conformation between subunits of glutamate-1-semialdehyde aminomutase in solution

scientific article published on 31 July 2013

Biochemical data from the characterization of a new pathogenic mutation of human pyridoxine-5'-phosphate oxidase (PNPO).

scientific article published on 28 October 2017

Biomedical aspects of pyridoxal 5 -phosphate availability ⬇️

scientific article published on January 1, 2012

Catalytic and thermodynamic properties of tetrahydromethanopterin-dependent serine hydroxymethyltransferase from Methanococcus jannaschii

scientific article published on 5 August 2003

Corrigendum to "Pyridoxine-5'-phosphate oxidase (Pnpo) deficiency: Clinical and biochemical alterations associated with the C.347g>A (P.·Arg116gln) mutation" [Mol. Genet. Metab. 122/1-2 (2017) 135-142]

scientific article published on 14 March 2018

Coupling bioorthogonal chemistries with artificial metabolism: intracellular biosynthesis of azidohomoalanine and its incorporation into recombinant proteins.

scientific article published on 15 January 2014

Crystal Structures of Human Pyridoxal Kinase in Complex with the Neurotoxins, Ginkgotoxin and Theophylline: Insights into Pyridoxal Kinase Inhibition

scientific article

Crystal structure of glutamate-1-semialdehyde aminomutase: an alpha2-dimeric vitamin B6-dependent enzyme with asymmetry in structure and active site reactivity

scientific article

Data from computational analysis of the peptide linkers in the MocR bacterial transcriptional regulators

scientific article

Differential 3-bromopyruvate inhibition of cytosolic and mitochondrial human serine hydroxymethyltransferase isoforms, key enzymes in cancer metabolic reprogramming.

scientific article published on 13 August 2016

Differential inhibitory effect of a pyrazolopyran compound on human serine hydroxymethyltransferase-amino acid complexes

scientific article published on 04 July 2018

Editorial: PLP-Dependent Enzymes: Extraordinary Versatile Catalysts and Ideal Biotechnological Tools for the Production of Unnatural Amino Acids and Related Compounds

scientific article published on 11 February 2020

Enzymatic synthesis of ω-carboxy-β-hydroxy-(l)-α-amino acids

Functional Inactivation of Drosophila GCK Orthologs Causes Genomic Instability and Oxidative Stress in a Fly Model of MODY-2 ⬇️

journal article from 'International Journal of Molecular Sciences' published in 2021

Glycine consumption and mitochondrial serine hydroxymethyltransferase in cancer cells: the heme connection

scientific article published on 6 March 2013

How pyridoxal 5'-phosphate differentially regulates human cytosolic and mitochondrial serine hydroxymethyltransferase oligomeric state

scientific article

Human Cytosolic and Mitochondrial Serine Hydroxymethyltransferase Isoforms in Comparison: Full Kinetic Characterization and Substrate Inhibition Properties

scientific article published on 07 December 2018

Identification and characterization of the pyridoxal 5’-phosphate allosteric site in Escherichia coli pyridoxine 5’-phosphate oxidase

scientific article published in 2021

In silico and in vitro validation of serine hydroxymethyltransferase as a chemotherapeutic target of the antifolate drug pemetrexed

scientific article (publication date: May 2011)

Inhibition of human pyridoxal kinase by 2-acetyl-4-((1R,2S,3R)-1,2,3,4-tetrahydroxybutyl)imidazole (THI).

scientific article

Interaction of Bacillus subtilis GabR with the gabTD promoter: role of repeated sequences and effect of GABA in transcriptional activation

scientific article published on 09 March 2020

Isolation of a Complex Formed Between HemA and HemL, Key Enzymes of Tetrapyrroles Biosynthesis

scientific article published on 26 February 2019

Molecular Basis of Reduced Pyridoxine 5′-Phosphate Oxidase Catalytic Activity in Neonatal Epileptic Encephalopathy Disorder

scientific article

Molecular basis of E. coli L-threonine aldolase catalytic inactivation at low pH.

scientific article

Molecular characterization of pyridoxine 5'-phosphate oxidase and its pathogenic forms associated with neonatal epileptic encephalopathy

scientific article published on 12 August 2020

Molecular mechanism of PdxR – a transcriptional activator involved in the regulation of vitamin B6 biosynthesis in the probiotic bacterium Bacillus clausii

scientific article published on 26 June 2015

Molecular mechanisms of the non-coenzyme action of thiamin in brain: biochemical, structural and pathway analysis.

scientific article published on 27 July 2015

Mutation of tyrosine 332 to phenylalanine converts dopa decarboxylase into a decarboxylation-dependent oxidative deaminase

scientific article

On the catalytic mechanism and stereospecificity ofEscherichia colil-threonine aldolase

scientific article

On the mechanism of Escherichia coli pyridoxal kinase inhibition by pyridoxal and pyridoxal 5'-phosphate

scientific article published on 3 February 2015

PLP-dependent enzymes

scientific article published on 15 January 2014

Pyridoxal 5'-phosphate enzymes as targets for therapeutic agents

scientific article published on January 2007

Pyridoxal 5'-phosphate is a slow tight binding inhibitor of E. coli pyridoxal kinase.

scientific article

Pyridoxine-5'-phosphate oxidase (Pnpo) deficiency: Clinical and biochemical alterations associated with the C.347g>A (P.·Arg116gln) mutation.

scientific article

Role of a conserved active site cation-pi interaction in Escherichia coli serine hydroxymethyltransferase

scientific article

SHMT1 knockdown induces apoptosis in lung cancer cells by causing uracil misincorporation.

scientific article

Salmonella typhimurium PtsJ is a novel MocR-like transcriptional repressor involved in regulating the vitamin B6 salvage pathway

scientific article

Screening and in vitro testing of antifolate inhibitors of human cytosolic serine hydroxymethyltransferase.

scientific article published on 10 February 2015

Serine hydroxymethyltransferase: a model enzyme for mechanistic, structural, and evolutionary studies

scientific article

Stereochemistry of the reactions of glutamate-1-semialdehyde aminomutase with 4,5-diaminovalerate

scientific article published on 23 July 2003

Structural and kinetic properties of serine hydroxymethyltransferase from the halophytic cyanobacterium Aphanothece halophytica provide a rationale for salt tolerance

scientific article published on 14 May 2020

Structural basis of methotrexate and pemetrexed action on serine hydroxymethyltransferases revealed using plant models

scientific article published on 23 December 2019

Structural properties of the linkers connecting the N- and C- terminal domains in the MocR bacterial transcriptional regulators.

scientific article

Structural stability of the cofactor binding site in Escherichia coli serine hydroxymethyltransferase--the role of evolutionarily conserved hydrophobic contacts.

scientific article

Structure-based mechanism for early PLP-mediated steps of rabbit cytosolic serine hydroxymethyltransferase reaction

scientific article published on 15 July 2013

Study of DNA binding and bending by Bacillus subtilis GabR, a PLP-dependent transcription factor

scientific article

The MocR-like transcription factors: pyridoxal 5'-phosphate-dependent regulators of bacterial metabolism

scientific article published on 05 July 2018

The aspartate aminotransferase-like domain of Firmicutes MocR transcriptional regulators

The catalytic activity of serine hydroxymethyltransferase is essential for de novo nuclear dTMP synthesis in lung cancer cells

scientific article published on 07 August 2018

The contribution of a conformationally mobile, active site loop to the reaction catalyzed by glutamate semialdehyde aminomutase

scientific article published on 01 February 2000

The crystal structure of archaeal serine hydroxymethyltransferase reveals idiosyncratic features likely required to withstand high temperatures

scientific article

The expression of four pyridoxal kinase (PDXK) human variants in Drosophila impacts on genome integrity

scientific article published on 02 October 2019

The mechanism of addition of pyridoxal 5'-phosphate to Escherichia coli apo-serine hydroxymethyltransferase

scientific article published on June 2007

The moonlighting RNA-binding activity of cytosolic serine hydroxymethyltransferase contributes to control compartmentalization of serine metabolism

scientific article published on 01 May 2019

The multifaceted role of vitamin B6 in cancer: Drosophila as a model system to investigate DNA damage

scientific article published on 25 March 2020

The role of evolutionarily conserved hydrophobic contacts in the quaternary structure stability of Escherichia coli serine hydroxymethyltransferase

scientific article published in January 2009

Threonine aldolase and alanine racemase: novel examples of convergent evolution in the superfamily of vitamin B6-dependent enzymes

scientific article

Vitamin B(6) salvage enzymes: mechanism, structure and regulation

scientific article published on 20 December 2010

l-Threonine aldolase, serine hydroxymethyltransferase and fungal alanine racemase. A subgroup of strictly related enzymes specialized for different functions

scientific article published on December 2001

List of works by Roberto Contestabile

A Bioinformatics Analysis Reveals a Group of MocR Bacterial Transcriptional Regulators Linked to a Family of Genes Coding for Membrane Proteins

A Comprehensive Computational Analysis of Mycobacterium Genomes Pinpoints the Genes Co-occurring with YczE, a Membrane Protein Coding Gene Under the Putative Control of a MocR, and Predicts its Function.

A pyrazolopyran derivative preferentially inhibits the activity of human cytosolic serine hydroxymethyltransferase and induces cell death in lung cancer cells.

Alanine racemase from Tolypocladium inflatum: A key PLP-dependent enzyme in cyclosporin biosynthesis and a model of catalytic promiscuity

Allosteric feedback inhibition of pyridoxine 5'-phosphate oxidase from Escherichia coli

An Engineered Escherichia coli Strain with Synthetic Metabolism for In-cell Production of Translationally Active Methionine Derivatives

Arabidopsis thaliana serine hydroxymethyltransferases: functions, structures, and perspectives

Asymmetry of the active site loop conformation between subunits of glutamate-1-semialdehyde aminomutase in solution

Biochemical data from the characterization of a new pathogenic mutation of human pyridoxine-5'-phosphate oxidase (PNPO).

Biomedical aspects of pyridoxal 5 -phosphate availability ⬇️

Catalytic and thermodynamic properties of tetrahydromethanopterin-dependent serine hydroxymethyltransferase from Methanococcus jannaschii

Corrigendum to "Pyridoxine-5'-phosphate oxidase (Pnpo) deficiency: Clinical and biochemical alterations associated with the C.347g>A (P.·Arg116gln) mutation" [Mol. Genet. Metab. 122/1-2 (2017) 135-142]

Coupling bioorthogonal chemistries with artificial metabolism: intracellular biosynthesis of azidohomoalanine and its incorporation into recombinant proteins.

Crystal Structures of Human Pyridoxal Kinase in Complex with the Neurotoxins, Ginkgotoxin and Theophylline: Insights into Pyridoxal Kinase Inhibition

Crystal structure of glutamate-1-semialdehyde aminomutase: an alpha2-dimeric vitamin B6-dependent enzyme with asymmetry in structure and active site reactivity

Data from computational analysis of the peptide linkers in the MocR bacterial transcriptional regulators

Differential 3-bromopyruvate inhibition of cytosolic and mitochondrial human serine hydroxymethyltransferase isoforms, key enzymes in cancer metabolic reprogramming.

Differential inhibitory effect of a pyrazolopyran compound on human serine hydroxymethyltransferase-amino acid complexes

Editorial: PLP-Dependent Enzymes: Extraordinary Versatile Catalysts and Ideal Biotechnological Tools for the Production of Unnatural Amino Acids and Related Compounds

Enzymatic synthesis of ω-carboxy-β-hydroxy-(l)-α-amino acids

Functional Inactivation of Drosophila GCK Orthologs Causes Genomic Instability and Oxidative Stress in a Fly Model of MODY-2 ⬇️

Glycine consumption and mitochondrial serine hydroxymethyltransferase in cancer cells: the heme connection

How pyridoxal 5'-phosphate differentially regulates human cytosolic and mitochondrial serine hydroxymethyltransferase oligomeric state

Human Cytosolic and Mitochondrial Serine Hydroxymethyltransferase Isoforms in Comparison: Full Kinetic Characterization and Substrate Inhibition Properties

Identification and characterization of the pyridoxal 5’-phosphate allosteric site in Escherichia coli pyridoxine 5’-phosphate oxidase

In silico and in vitro validation of serine hydroxymethyltransferase as a chemotherapeutic target of the antifolate drug pemetrexed

Inhibition of human pyridoxal kinase by 2-acetyl-4-((1R,2S,3R)-1,2,3,4-tetrahydroxybutyl)imidazole (THI).

Interaction of Bacillus subtilis GabR with the gabTD promoter: role of repeated sequences and effect of GABA in transcriptional activation

Isolation of a Complex Formed Between HemA and HemL, Key Enzymes of Tetrapyrroles Biosynthesis

Molecular Basis of Reduced Pyridoxine 5′-Phosphate Oxidase Catalytic Activity in Neonatal Epileptic Encephalopathy Disorder

Molecular basis of E. coli L-threonine aldolase catalytic inactivation at low pH.

Molecular characterization of pyridoxine 5'-phosphate oxidase and its pathogenic forms associated with neonatal epileptic encephalopathy

Molecular mechanism of PdxR – a transcriptional activator involved in the regulation of vitamin B6 biosynthesis in the probiotic bacterium Bacillus clausii

Molecular mechanisms of the non-coenzyme action of thiamin in brain: biochemical, structural and pathway analysis.

Mutation of tyrosine 332 to phenylalanine converts dopa decarboxylase into a decarboxylation-dependent oxidative deaminase

On the catalytic mechanism and stereospecificity ofEscherichia colil-threonine aldolase

On the mechanism of Escherichia coli pyridoxal kinase inhibition by pyridoxal and pyridoxal 5'-phosphate

PLP-dependent enzymes

Pyridoxal 5'-phosphate enzymes as targets for therapeutic agents

Pyridoxal 5'-phosphate is a slow tight binding inhibitor of E. coli pyridoxal kinase.

Pyridoxine-5'-phosphate oxidase (Pnpo) deficiency: Clinical and biochemical alterations associated with the C.347g>A (P.·Arg116gln) mutation.

Role of a conserved active site cation-pi interaction in Escherichia coli serine hydroxymethyltransferase

SHMT1 knockdown induces apoptosis in lung cancer cells by causing uracil misincorporation.

Salmonella typhimurium PtsJ is a novel MocR-like transcriptional repressor involved in regulating the vitamin B6 salvage pathway

Screening and in vitro testing of antifolate inhibitors of human cytosolic serine hydroxymethyltransferase.

Serine hydroxymethyltransferase: a model enzyme for mechanistic, structural, and evolutionary studies

Stereochemistry of the reactions of glutamate-1-semialdehyde aminomutase with 4,5-diaminovalerate

Structural and kinetic properties of serine hydroxymethyltransferase from the halophytic cyanobacterium Aphanothece halophytica provide a rationale for salt tolerance

Structural basis of methotrexate and pemetrexed action on serine hydroxymethyltransferases revealed using plant models

Structural properties of the linkers connecting the N- and C- terminal domains in the MocR bacterial transcriptional regulators.

Structural stability of the cofactor binding site in Escherichia coli serine hydroxymethyltransferase--the role of evolutionarily conserved hydrophobic contacts.

Structure-based mechanism for early PLP-mediated steps of rabbit cytosolic serine hydroxymethyltransferase reaction

Study of DNA binding and bending by Bacillus subtilis GabR, a PLP-dependent transcription factor

The MocR-like transcription factors: pyridoxal 5'-phosphate-dependent regulators of bacterial metabolism

The aspartate aminotransferase-like domain of Firmicutes MocR transcriptional regulators

The catalytic activity of serine hydroxymethyltransferase is essential for de novo nuclear dTMP synthesis in lung cancer cells

The contribution of a conformationally mobile, active site loop to the reaction catalyzed by glutamate semialdehyde aminomutase

The crystal structure of archaeal serine hydroxymethyltransferase reveals idiosyncratic features likely required to withstand high temperatures

The expression of four pyridoxal kinase (PDXK) human variants in Drosophila impacts on genome integrity

The mechanism of addition of pyridoxal 5'-phosphate to Escherichia coli apo-serine hydroxymethyltransferase

The moonlighting RNA-binding activity of cytosolic serine hydroxymethyltransferase contributes to control compartmentalization of serine metabolism

The multifaceted role of vitamin B6 in cancer: Drosophila as a model system to investigate DNA damage

The role of evolutionarily conserved hydrophobic contacts in the quaternary structure stability of Escherichia coli serine hydroxymethyltransferase

Threonine aldolase and alanine racemase: novel examples of convergent evolution in the superfamily of vitamin B6-dependent enzymes

Vitamin B(6) salvage enzymes: mechanism, structure and regulation

l-Threonine aldolase, serine hydroxymethyltransferase and fungal alanine racemase. A subgroup of strictly related enzymes specialized for different functions