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List of works by Julio Polaina

A fast procedure for yeast DNA purification

scientific article

A purple acidophilic di-ferric DNA ligase from Ferroplasma

scholarly article

A search for hyperglycosylation signals in yeast glycoproteins

scientific article published on 26 July 2004

Additive effect of single amino acid replacements on the kinetic stability of β-glucosidase B

scientific article published on 01 October 2012

Analysis of Domain Architecture and Phylogenetics of Family 2 Glycoside Hydrolases (GH2)

scientific article

Analysis of a polygalacturonase gene of Ustilago maydis and characterization of the encoded enzyme

scientific article published on 20 May 2013

Biochemical diversity of carboxyl esterases and lipases from Lake Arreo (Spain): a metagenomic approach

scientific article (publication date: June 2013)

Catalytic role of conserved HQGE motif in the CE6 carbohydrate esterase family

scientific article published on 04 September 2007

Changes in the publication system for World Journal of Microbiology and Biotechnology

scientific article published on 01 January 2016

Characterization of a beta-fructofuranosidase from Schwanniomyces occidentalis with transfructosylating activity yielding the prebiotic 6-kestose.

scientific article published on 26 July 2007

Characterization of the transcriptional regulator Rv3124 of Mycobacterium tuberculosis identifies it as a positive regulator of molybdopterin biosynthesis and defines the functional consequences of a non-synonymous SNP in the Mycobacterium [...]

scientific journal article

Cloning and molecular characterization of the delta6-desaturase from two echium plant species: production of GLA by heterologous expression in yeast and tobacco.

scientific article published in April 2002

Cloning of the STA2 and SGA genes encoding glucoamylases in yeasts and regulation of their expression by the STA10 gene of Saccharomyces cerevisiae

scientific article

Combinatorial saturation mutagenesis by in vivo overlap extension for the engineering of fungal laccases

scientific article

Combinatorial saturation mutagenesis of the Myceliophthora thermophila laccase T2 mutant: the connection between the C-terminal plug and the conserved (509)VSG(511) tripeptide

scientific article

Comparative study and mutational analysis of distinctive structural elements of hyperthermophilic enzymes

scientific article

Construction of a Saccharomyces cerevisiae strain able to ferment cellobiose

scientific article published on 01 July 1991

Conversion of a carboxylesterase into a triacylglycerol lipase by a random mutation

scientific article published on 01 November 2005

Crystal structure of β-glucosidase A from Bacillus polymyxa: insights into the catalytic activity in family 1 glycosyl hydrolases ⬇️

scientific article published on January 23, 1998

Crystal structures of Paenibacillus polymyxa beta-glucosidase B complexes reveal the molecular basis of substrate specificity and give new insights into the catalytic machinery of family I glycosidases

scientific article

Crystallization and preliminary X-ray diffraction analysis of a type I beta-glucosidase encoded by the bgIA gene of Bacillus polymyxa.

scientific article

Crystallization and preliminary X-ray diffraction analysis of the invertase from Saccharomyces cerevisiae

scientific article published on 19 November 2012

Development of enzymatically-active bacterial cellulose membranes through stable immobilization of an engineered β-galactosidase

scientific article published on 18 April 2018

Directed evolution of beta -glucosidase A from Paenibacillus polymyxa to thermal resistance

scientific article published in May 2000

Dissecting mRNA decay and translation inhibition during iron deficiency

scientific article published on 20 August 2018

Effect of a new ionic pair on the unfolding activation barrier of beta-glucosidase B.

scientific article published in January 2006

Enzymatic Synthesis of a Novel Pterostilbene α-Glucoside by the Combination of Cyclodextrin Glucanotransferase and Amyloglucosidase.

scientific article published on 25 May 2018

Fermentation of cellobiose to ethanol by industrial Saccharomyces strains carrying the β-glucosidase gene (BGL1) from Saccharomycopsis fibuligera

article

Fructo-oligosaccharide synthesis by mutant versions of Saccharomyces cerevisiae invertase

scientific article

Functional metagenomics unveils a multifunctional glycosyl hydrolase from the family 43 catalysing the breakdown of plant polymers in the calf rumen.

scientific article

Genes involved in the control of nuclear fusion during the sexual cycle of Saccharomyces cerevisiae

scientific article published on 01 January 1982

Glucoamylases: structural and biotechnological aspects ⬇️

scientific article published on 09 December 2010

Highly efficient assimilation of lactose by a metabolically engineered strain of Saccharomyces cerevisiae

scientific article published on 01 June 1998

Identification and Structural Analysis of Amino Acid Substitutions that Increase the Stability and Activity of Aspergillus niger Glucose Oxidase

scientific article

Immobilization of thermostable β-galactosidase on epoxy support and its use for lactose hydrolysis and galactooligosaccharides biosynthesis

article

Improving the amylolytic activity of Saccharomyces cerevisiae glucoamylase by the addition of a starch binding domain.

scientific article published in August 2005

In vitro evolution of a fungal laccase in high concentrations of organic cosolvents.

scientific article

Induced expression of bacterial beta-glucosidase activity in Saccharomyces

scientific article published on 01 April 1995

Inter‐conversion of catalytic abilities in a bifunctional carboxyl/feruloyl‐esterase from earthworm gut metagenome ⬇️

scientific article published on July 17, 2009

Lactose utilization by Saccharomyces cerevisiae strains expressing Kluyveromyces lactis LAC genes.

scientific article published in November 2001

Microbial β-glucosidases from cow rumen metagenome enhance the saccharification of lignocellulose in combination with commercial cellulase cocktail ⬇️

scientific article published on September 21, 2012

Molecular and structural modeling of the Phanerochaete flavido-alba extracellular laccase reveals its ferroxidase structure.

scientific article

Novel hybrid esterase-haloacid dehalogenase enzyme

scientific article published on 01 September 2010

Novel polyphenol oxidase mined from a metagenome expression library of bovine rumen: biochemical properties, structural analysis, and phylogenetic relationships.

scientific article

One-pot production of fructooligosaccharides by a Saccharomyces cerevisiae strain expressing an engineered invertase.

scientific article

PsPMEP, a pollen-specific pectin methylesterase of pea (Pisum sativum L.).

scientific article published on 10 July 2013

Random mutagenesis of a plasmid-borne glycosidase gene and phenotypic selection of mutants in Escherichia coli.

scientific article

Rtp1p is a karyopherin-like protein required for RNA polymerase II biogenesis.

scientific article

STA10: A gene involved in the control of starch utilization by Saccharomyces

scientific article published on 01 April 1983

Self-diploidization in Saccharomyces cerevisiae kar2 heterokaryons

scientific article published on 01 November 1993

Sequences and homology analysis of two genes encoding beta-glucosidases from Bacillus polymyxa

scientific article published in October 1990

Structural Dissection of the Active Site of Thermotoga maritima β-Galactosidase Identifies Key Residues for Transglycosylating Activity

scientific article published on 21 March 2016

Structural analysis of glucoamylase encoded by the STA1 gene of Saccharomyces cerevisiae (var. diastaticus).

scientific article published in April 2004

Structural and functional analysis of hybrid enzymes generated by domain shuffling between Saccharomyces cerevisiae (var. diastaticus) Sta1 glucoamylase and Saccharomycopsis fibuligera Bgl1 β-glucosidase

scientific article published on 07 September 2010

Structural and functional characterization of a ruminal β-glycosidase defines a novel subfamily of glycosyl hydrolase family 3 with permuted domain topology

scientific article

Structural basis of the inhibition of GH1 β-glucosidases by multivalent pyrrolidine iminosugars

scientific article published on 04 June 2019

Structural hierarchy of regulatory elements in the folding and transport of an intestinal multidomain protein.

scientific article

Structure-function analysis of yeast Grx5 monothiol glutaredoxin defines essential amino acids for the function of the protein.

scientific article published on 22 July 2002

Synergies in coupled hydrolysis and fermentation of cellulose using a Trichoderma reesei enzyme preparation and a recombinant Saccharomyces cerevisiae strain

scientific article published on 06 June 2017

Synthesis of Isomaltooligosaccharides by Saccharomyces cerevisiae Cells Expressing Aspergillus niger α-Glucosidase.

scientific article published on 16 November 2017

The cryo-EM Structure of Thermotoga maritima β-Galactosidase: Quaternary Structure Guides Protein Engineering

scientific article published on 24 December 2019

The ribosomal DNA of the Zygomycete Mucor miehei

scientific article

Transformation of Mucor miehei results in plasmid deletion and phenotypic instability

scientific article

Use of the polyene antibiotic N-glycosyl-polifungin in counterselecting yeast mutants

scientific article published on 01 March 1981

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