List of works - Matthew P. DeLisa

A cell-free biosynthesis platform for modular construction of protein glycosylation pathways

scientific article published on 27 November 2019

A cell-free platform for rapid synthesis and testing of active oligosaccharyltransferases

scientific article published on 27 November 2017

A filamentous phage display system for N-linked glycoproteins.

scientific article

A little help from my friends: quality control of presecretory proteins in bacteria

scientific article

A prokaryote-based cell-free translation system that efficiently synthesizes glycoproteins.

scientific article

A survival selection strategy for engineering synthetic binding proteins that specifically recognize post-translationally phosphorylated proteins

scientific article published on 23 April 2019

A three-channel microfluidic device for generating static linear gradients and its application to the quantitative analysis of bacterial chemotaxis

scientific article published on 13 December 2005

A water-soluble DsbB variant that catalyzes disulfide-bond formation in vivo

scientific article

An Engineered Survival-Selection Assay for Extracellular Protein Expression Uncovers Hypersecretory Phenotypes in Escherichia coli

scientific article

An engineered eukaryotic protein glycosylation pathway in Escherichia coli.

scientific article

An engineered genetic selection for ternary protein complexes inspired by a natural three-component hitchhiker mechanism.

scientific article published on 22 December 2014

An engineered pathway for the formation of protein disulfide bonds

scientific article published in February 2004

An essential role for the DnaK molecular chaperone in stabilizing over-expressed substrate proteins of the bacterial twin-arginine translocation pathway

scientific article published on 12 January 2007

Author Correction: Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery

scientific article published in Nature Communications

Bacterial autoinduction: looking outside the cell for new metabolic engineering targets

scientific article

Beyond the cytoplasm of Escherichia coli: localizing recombinant proteins where you want them

scientific article

Broad-Spectrum Proteome Editing with an Engineered Bacterial Ubiquitin Ligase Mimic

scientific article published on 10 May 2019

Characterizing Metal-Dependent Nucleases of CRISPR-Cas Prokaryotic Adaptive Immunity Systems.

scientific article published in January 2015

Chlamydia trachomatis secretion of an immunodominant hypothetical protein (CT795) into host cell cytoplasm.

scientific article

Collective bacterial dynamics revealed using a three-dimensional population-scale defocused particle tracking technique

scientific article

Computational affinity maturation of camelid single-domain intrabodies against the nonamyloid component of alpha-synuclein

scientific article published in Scientific Reports

Creation of artificial cellulosomes on DNA scaffolds by zinc finger protein-guided assembly for efficient cellulose hydrolysis.

scientific article published in February 2014

DNA microarray-based identification of genes controlled by autoinducer 2-stimulated quorum sensing in Escherichia coli

scientific article

DNA-guided assembly of biosynthetic pathways promotes improved catalytic efficiency

scientific article published on 22 October 2011

Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases

scientific article published on 07 May 2018

Directed evolution of Mycobacterium tuberculosis β-lactamase reveals gatekeeper residue that regulates antibiotic resistance and catalytic efficiency

scientific article

Discovery of amyloid-beta aggregation inhibitors using an engineered assay for intracellular protein folding and solubility

scientific article

Dissecting the twin-arginine translocation pathway using genome-wide analysis.

scientific article

Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria.

scientific article

Efficient isolation of soluble intracellular single-chain antibodies using the twin-arginine translocation machinery

scientific article

Engineered Protein Machines: Emergent Tools for Synthetic Biology

scientific article published on January 2016

Engineered genetic selection links in vivo protein folding and stability with asparagine-linked glycosylation

scientific article published on 4 October 2013

Engineered oligosaccharyltransferases with greatly relaxed acceptor-site specificity

scientific article

Engineering a Supersecreting Strain of <i>Escherichia coli</i> by Directed Coevolution of the Multiprotein Tat Translocation Machinery

scientific article published in 2021

Engineering antibody fitness and function using membrane-anchored display of correctly folded proteins.

scientific article

Engineering orthogonal human O-linked glycoprotein biosynthesis in bacteria

scientific article published on 27 July 2020

Engineering the protein folding landscape in gram-negative bacteria.

scientific article published on April 2008

Engineering the spatial organization of metabolic enzymes: mimicking nature's synergy

scientific article

Evanescent wave long-period fiber bragg grating as an immobilized antibody biosensor

scientific article published on 01 July 2000

Expanding the glycoengineering toolbox: the rise of bacterial N-linked protein glycosylation

scientific article published on 11 April 2013

Exploration of twin-arginine translocation for expression and purification of correctly folded proteins in Escherichia coli

scientific article published on September 2008

Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides

scientific article published on 11 January 2007

Following the path of a twin-arginine precursor along the TatABC translocase of Escherichia coli

scientific article published on 3 October 2008

Functional reconstitution of a tunable E3-dependent sumoylation pathway in Escherichia coli.

scientific article

Genetic selection for protein solubility enabled by the folding quality control feature of the twin-arginine translocation pathway

scientific article

Genetic selection of solubility-enhanced proteins using the twin-arginine translocation system

scientific article published on January 2011

Genetic toggling of alkaline phosphatase folding reveals signal peptides for all major modes of transport across the inner membrane of bacteria

scientific article

Glycans-by-design: engineering bacteria for the biosynthesis of complex glycans and glycoconjugates

scientific article published on 26 March 2013

GlycoSNAP: A High-Throughput Screening Methodology for Engineering Designer Glycosylation Enzymes.

scientific article published on January 2015

Glycoarrays with engineered phages displaying structurally diverse oligosaccharides enable high-throughput detection of glycan-protein interactions

scientific article

High-Throughput Synthesis and Analysis of Intact Glycoproteins Using SAMDI-MS

scientific article published on 07 January 2020

Identification of a twin-arginine translocation system in Pseudomonas syringae pv. tomato DC3000 and its contribution to pathogenicity and fitness

scientific article

Identifying and optimizing intracellular protein-protein interactions using bacterial genetic selection.

scientific article

Immunization with Outer Membrane Vesicles Displaying Designer Glycotopes Yields Class-Switched, Glycan-Specific Antibodies

scientific article published on June 2016

Immunization with outer membrane vesicles displaying conserved surface polysaccharide antigen elicits broadly antimicrobial antibodies.

scientific article published on 19 March 2018

In silico protein fragmentation reveals the importance of critical nuclei on domain reassembly.

scientific article

Intracellular ribosome display via SecM translation arrest as a selection for antibodies with enhanced cytosolic stability

scientific article published on 3 July 2007

Kinetics and reaction coordinates of the reassembly of protein fragments via forward flux sampling.

scientific article

Laboratory evolution of fast-folding green fluorescent protein using secretory pathway quality control

scientific article

LuxS coexpression enhances yields of recombinant proteins in Escherichia coli in part through posttranscriptional control of GroEL.

scientific article

Making water-soluble integral membrane proteins in vivo using an amphipathic protein fusion strategy

scientific article

Mapping stress-induced changes in autoinducer AI-2 production in chemostat-cultivated Escherichia coli K-12.

scientific article published on May 2001

Mechanistic insight into the TH1-biased immune response to recombinant subunit vaccines delivered by probiotic bacteria-derived outer membrane vesicles

scientific article

Microbial biosynthesis of designer outer membrane vesicles.

scientific article published on 22 March 2014

Mining mammalian genomes for folding competent proteins using Tat-dependent genetic selection in Escherichia coli

scientific article published on December 2009

Optimizing recombinant antibodies for intracellular function using hitchhiker-mediated survival selection

scientific article

Pathogen-like particles: biomimetic vaccine carriers engineered at the nanoscale.

scientific article published on 07 December 2013

Phage shock protein PspA of Escherichia coli relieves saturation of protein export via the Tat pathway

scientific article published in January 2004

Positional assembly of enzymes on bacterial outer membrane vesicles for cascade reactions

scientific article

Production of secretory and extracellular N-linked glycoproteins in Escherichia coli.

scientific article

Protein translocation through a tunnel induces changes in folding kinetics: a lattice model study

scientific article published in May 2006

Quorum signaling via AI-2 communicates the "Metabolic Burden" associated with heterologous protein production in Escherichia coli

scientific article published in November 2001

Recombinant M2e outer membrane vesicle vaccines protect against lethal influenza A challenge in BALB/c mice.

scientific article published on 28 January 2016

Repurposing a bacterial quality control mechanism to enhance enzyme production in living cells

scientific article published on 12 January 2015

Secretion of the chlamydial virulence factor CPAF requires the Sec-dependent pathway

scientific article

Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery.

scientific article published on 12 July 2018

Site-specific labeling of surface proteins on living cells using genetically encoded peptides that bind fluorescent nanoparticle probes.

scientific article

Split-Cre recombinase effectively monitors protein-protein interactions in living bacteria

scientific article published on 29 January 2014

Stochastic reaction-diffusion simulation of enzyme compartmentalization reveals improved catalytic efficiency for a synthetic metabolic pathway.

scientific article published on 26 May 2007

Substitute sweeteners: diverse bacterial oligosaccharyltransferases with unique N-glycosylation site preferences

scientific article published on 20 October 2015

Synthetic metabolic pipelines

scientific article published in August 2009

The ribosomal exit tunnel as a target for optimizing protein expression in Escherichia coli

scientific article published on March 2012

Twin-arginine translocation of active human tissue plasminogen activator in Escherichia coli

scientific article published on December 2005

Ubiquibodies, synthetic E3 ubiquitin ligases endowed with unnatural substrate specificity for targeted protein silencing

scientific article published on 28 January 2014

Universal genetic assay for engineering extracellular protein expression.

scientific article

Versatile selection technology for intracellular protein-protein interactions mediated by a unique bacterial hitchhiker transport mechanism

scientific article

Visualizing interactions along the Escherichia coli twin-arginine translocation pathway using protein fragment complementation

scientific article

List of works by Matthew P. DeLisa

A cell-free biosynthesis platform for modular construction of protein glycosylation pathways

A cell-free platform for rapid synthesis and testing of active oligosaccharyltransferases

A filamentous phage display system for N-linked glycoproteins.

A little help from my friends: quality control of presecretory proteins in bacteria

A prokaryote-based cell-free translation system that efficiently synthesizes glycoproteins.

A survival selection strategy for engineering synthetic binding proteins that specifically recognize post-translationally phosphorylated proteins

A three-channel microfluidic device for generating static linear gradients and its application to the quantitative analysis of bacterial chemotaxis

A water-soluble DsbB variant that catalyzes disulfide-bond formation in vivo

An Engineered Survival-Selection Assay for Extracellular Protein Expression Uncovers Hypersecretory Phenotypes in Escherichia coli

An engineered eukaryotic protein glycosylation pathway in Escherichia coli.

An engineered genetic selection for ternary protein complexes inspired by a natural three-component hitchhiker mechanism.

An engineered pathway for the formation of protein disulfide bonds

An essential role for the DnaK molecular chaperone in stabilizing over-expressed substrate proteins of the bacterial twin-arginine translocation pathway

Author Correction: Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery

Bacterial autoinduction: looking outside the cell for new metabolic engineering targets

Beyond the cytoplasm of Escherichia coli: localizing recombinant proteins where you want them

Broad-Spectrum Proteome Editing with an Engineered Bacterial Ubiquitin Ligase Mimic

Characterizing Metal-Dependent Nucleases of CRISPR-Cas Prokaryotic Adaptive Immunity Systems.

Chlamydia trachomatis secretion of an immunodominant hypothetical protein (CT795) into host cell cytoplasm.

Collective bacterial dynamics revealed using a three-dimensional population-scale defocused particle tracking technique

Computational affinity maturation of camelid single-domain intrabodies against the nonamyloid component of alpha-synuclein

Creation of artificial cellulosomes on DNA scaffolds by zinc finger protein-guided assembly for efficient cellulose hydrolysis.

DNA microarray-based identification of genes controlled by autoinducer 2-stimulated quorum sensing in Escherichia coli

DNA-guided assembly of biosynthetic pathways promotes improved catalytic efficiency

Design of glycosylation sites by rapid synthesis and analysis of glycosyltransferases

Directed evolution of Mycobacterium tuberculosis β-lactamase reveals gatekeeper residue that regulates antibiotic resistance and catalytic efficiency

Discovery of amyloid-beta aggregation inhibitors using an engineered assay for intracellular protein folding and solubility

Dissecting the twin-arginine translocation pathway using genome-wide analysis.

Efficient expression of full-length antibodies in the cytoplasm of engineered bacteria.

Efficient isolation of soluble intracellular single-chain antibodies using the twin-arginine translocation machinery

Engineered Protein Machines: Emergent Tools for Synthetic Biology

Engineered genetic selection links in vivo protein folding and stability with asparagine-linked glycosylation

Engineered oligosaccharyltransferases with greatly relaxed acceptor-site specificity

Engineering a Supersecreting Strain of <i>Escherichia coli</i> by Directed Coevolution of the Multiprotein Tat Translocation Machinery

Engineering antibody fitness and function using membrane-anchored display of correctly folded proteins.

Engineering orthogonal human O-linked glycoprotein biosynthesis in bacteria

Engineering the protein folding landscape in gram-negative bacteria.

Engineering the spatial organization of metabolic enzymes: mimicking nature's synergy

Evanescent wave long-period fiber bragg grating as an immobilized antibody biosensor

Expanding the glycoengineering toolbox: the rise of bacterial N-linked protein glycosylation

Exploration of twin-arginine translocation for expression and purification of correctly folded proteins in Escherichia coli

Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides

Following the path of a twin-arginine precursor along the TatABC translocase of Escherichia coli

Functional reconstitution of a tunable E3-dependent sumoylation pathway in Escherichia coli.

Genetic selection for protein solubility enabled by the folding quality control feature of the twin-arginine translocation pathway

Genetic selection of solubility-enhanced proteins using the twin-arginine translocation system

Genetic toggling of alkaline phosphatase folding reveals signal peptides for all major modes of transport across the inner membrane of bacteria

Glycans-by-design: engineering bacteria for the biosynthesis of complex glycans and glycoconjugates

GlycoSNAP: A High-Throughput Screening Methodology for Engineering Designer Glycosylation Enzymes.

Glycoarrays with engineered phages displaying structurally diverse oligosaccharides enable high-throughput detection of glycan-protein interactions

High-Throughput Synthesis and Analysis of Intact Glycoproteins Using SAMDI-MS

Identification of a twin-arginine translocation system in Pseudomonas syringae pv. tomato DC3000 and its contribution to pathogenicity and fitness

Identifying and optimizing intracellular protein-protein interactions using bacterial genetic selection.

Immunization with Outer Membrane Vesicles Displaying Designer Glycotopes Yields Class-Switched, Glycan-Specific Antibodies

Immunization with outer membrane vesicles displaying conserved surface polysaccharide antigen elicits broadly antimicrobial antibodies.

In silico protein fragmentation reveals the importance of critical nuclei on domain reassembly.

Intracellular ribosome display via SecM translation arrest as a selection for antibodies with enhanced cytosolic stability

Kinetics and reaction coordinates of the reassembly of protein fragments via forward flux sampling.

Laboratory evolution of fast-folding green fluorescent protein using secretory pathway quality control

LuxS coexpression enhances yields of recombinant proteins in Escherichia coli in part through posttranscriptional control of GroEL.

Making water-soluble integral membrane proteins in vivo using an amphipathic protein fusion strategy

Mapping stress-induced changes in autoinducer AI-2 production in chemostat-cultivated Escherichia coli K-12.

Mechanistic insight into the TH1-biased immune response to recombinant subunit vaccines delivered by probiotic bacteria-derived outer membrane vesicles

Microbial biosynthesis of designer outer membrane vesicles.

Mining mammalian genomes for folding competent proteins using Tat-dependent genetic selection in Escherichia coli

Optimizing recombinant antibodies for intracellular function using hitchhiker-mediated survival selection

Pathogen-like particles: biomimetic vaccine carriers engineered at the nanoscale.

Phage shock protein PspA of Escherichia coli relieves saturation of protein export via the Tat pathway

Positional assembly of enzymes on bacterial outer membrane vesicles for cascade reactions

Production of secretory and extracellular N-linked glycoproteins in Escherichia coli.

Protein translocation through a tunnel induces changes in folding kinetics: a lattice model study

Quorum signaling via AI-2 communicates the "Metabolic Burden" associated with heterologous protein production in Escherichia coli

Recombinant M2e outer membrane vesicle vaccines protect against lethal influenza A challenge in BALB/c mice.

Repurposing a bacterial quality control mechanism to enhance enzyme production in living cells

Secretion of the chlamydial virulence factor CPAF requires the Sec-dependent pathway

Single-pot glycoprotein biosynthesis using a cell-free transcription-translation system enriched with glycosylation machinery.

Site-specific labeling of surface proteins on living cells using genetically encoded peptides that bind fluorescent nanoparticle probes.

Split-Cre recombinase effectively monitors protein-protein interactions in living bacteria

Stochastic reaction-diffusion simulation of enzyme compartmentalization reveals improved catalytic efficiency for a synthetic metabolic pathway.