List of works - Andreas Schmid

A rapid, reliable, and automatable lab-on-a-chip interface

scientific article

A three-step method for analysing bacterial biofilm formation under continuous medium flow

scientific article

An artificial TCA cycle selects for efficient α-ketoglutarate dependent hydroxylase catalysis in engineered Escherichia coli

scientific article published on 7 March 2017

Analysis of carbon and nitrogen co-metabolism in yeast by ultrahigh-resolution mass spectrometry applying 13C- and 15N-labeled substrates simultaneously

scientific article published on 29 April 2012

Analytical biotechnology: from single molecule and single cell analyses to population dynamics of metabolites and cells

scientific article published on 25 February 2010

Biofilms as living catalysts in continuous chemical syntheses

scientific article published on 15 June 2012

Carbon metabolism and product inhibition determine the epoxidation efficiency of solvent-tolerant Pseudomonas sp. strain VLB120DeltaC.

scientific article published in December 2007

Carbon metabolism limits recombinant protein production in Pichia pastoris

scientific article

Cell physiology rather than enzyme kinetics can determine the efficiency of cytochrome P450-catalyzed C-H-oxyfunctionalization

scientific article published on 11 May 2011

Challenging biological limits with microfluidic single cell analysis

scientific article published on January 2015

Characterization of a biofilm membrane reactor and its prospects for fine chemical synthesis

scientific article published in March 2010

Chemical and biological single cell analysis

scientific article

Comparison of microbial hosts and expression systems for mammalian CYP1A1 catalysis

scientific article published on 24 August 2011

Complete genome sequence of Pseudomonas sp. strain VLB120 a solvent tolerant, styrene degrading bacterium, isolated from forest soil

scientific article published on 24 October 2013

Constitutively solvent-tolerant Pseudomonas taiwanensis VLB120∆ C∆ ttgV supports particularly high-styrene epoxidation activities when grown under glucose excess conditions

scientific article published on 06 February 2019

Continuous cyclohexane oxidation to cyclohexanol using a novel cytochrome P450 monooxygenase from Acidovorax sp. CHX100 in recombinant P. taiwanensis VLB120 biofilms

scientific article

Continuous multistep synthesis of perillic acid from limonene by catalytic biofilms under segmented flow

scientific article published on 17 August 2016

Coupling limonene formation and oxyfunctionalization by mixed-culture resting cell fermentation.

scientific article

D-Xylose assimilation via the Weimberg pathway by solvent-tolerant Pseudomonas taiwanensis VLB120.

scientific article published on 15 July 2014

Decoupling production from growth by magnesium sulfate limitation boosts de novo limonene production

scientific article

Detection of volatile metabolites of Escherichia coli by multi capillary column coupled ion mobility spectrometry

scientific article

Efficient hydroxyproline production from glucose in minimal media by Corynebacterium glutamicum.

scientific article published on 21 October 2014

Efficient phase separation and product recovery in organic-aqueous bioprocessing using supercritical carbon dioxide

scientific article published in November 2010

Efficient production of the Nylon 12 monomer ω-aminododecanoic acid methyl ester from renewable dodecanoic acid methyl ester with engineered Escherichia coli.

scientific article

Enantioselective substrate binding in a monooxygenase protein model by molecular dynamics and docking.

scientific article

Engineered catalytic biofilms for continuous large scale production of n-octanol and (S)-styrene oxide

scientific article published on 10 December 2012

Engineering of Pseudomonas taiwanensis VLB120 for constitutive solvent tolerance and increased specific styrene epoxidation activity

scientific article

Engineering the productivity of recombinant Escherichia coli for limonene formation from glycerol in minimal media.

scientific article published on 3 June 2014

Enzyme catalysis in an aqueous/organic segment flow microreactor: ways to stabilize enzyme activity

scientific article published on 01 June 2010

Glycerophospholipid profiling by high-performance liquid chromatography/mass spectrometry using exact mass measurements and multi-stage mass spectrometric fragmentation experiments in parallel

scientific article

Guidelines for reporting of biocatalytic reactions

scientific article published on 10 February 2010

Guiding bioprocess design by microbial ecology

scientific article published on 30 March 2015

Guiding efficient microbial synthesis of non-natural chemicals by physicochemical properties of reactants

scientific article published on 30 March 2015

Heme-iron oxygenases: powerful industrial biocatalysts?

scientific article published on 04 March 2008

Hsp90 regulates the dynamics of its cochaperone Sti1 and the transfer of Hsp70 between modules

scientific article published on 8 April 2015

Hydrolase BioH knockout in E. coli enables efficient fatty acid methyl ester bioprocessing.

scientific article published on 24 December 2016

Integrated one-pot enrichment and immobilization of styrene monooxygenase (StyA) using SEPABEAD EC-EA and EC-Q1A anion-exchange carriers.

scientific article published on 18 July 2011

Integrated organic–aqueous biocatalysis and product recovery for quinaldine hydroxylation catalyzed by living recombinant Pseudomonas putida

scientific article published on 02 March 2012

Isolated microbial single cells and resulting micropopulations grow faster in controlled environments

scientific article published on 20 July 2012

Making variability less variable: matching expression system and host for oxygenase-based biotransformations.

scientific article published on 16 April 2015

Maximization of cell viability rather than biocatalyst activity improves whole-cell ω-oxyfunctionalization performance.

scientific article published on 7 November 2016

Maximizing the productivity of catalytic biofilms on solid supports in membrane aerated reactors

scientific article published in July 2010

Metabolic and transcriptional response to cofactor perturbations in Escherichia coli.

scientific article published on 18 March 2010

Metabolic capacity estimation of Escherichia coli as a platform for redox biocatalysis: constraint-based modeling and experimental verification.

scientific article published in August 2008

Metabolic engineering of Pseudomonas sp. strain VLB120 as platform biocatalyst for the production of isobutyric acid and other secondary metabolites

scientific article (publication date: 7 January 2014)

Metabolic flux analysis of a phenol producing mutant of Pseudomonas putida S12: verification and complementation of hypotheses derived from transcriptomics.

scientific article published on 26 June 2009

Metabolic network capacity of Escherichia coli for Krebs cycle-dependent proline hydroxylation.

scientific article published on 29 July 2015

Metabolic response of Pseudomonas putida during redox biocatalysis in the presence of a second octanol phase.

scientific article

Microbial biofilms: a concept for industrial catalysis?

scientific article published on 23 September 2009

Microbial biofilms: new catalysts for maximizing productivity of long-term biotransformations

scientific article published in December 2007

Microfluidic single-cell analysis links boundary environments and individual microbial phenotypes

scientific article

Monitoring and control of microbioreactors: an expert opinion on development needs

scientific article published on 11 September 2012

NADH availability limits asymmetric biocatalytic epoxidation in a growing recombinant Escherichia coli strain.

scientific article published on 11 January 2008

Novel cyclohexane monooxygenase from Acidovorax sp. CHX100.

scientific article published on 3 May 2015

Outer membrane protein AlkL boosts biocatalytic oxyfunctionalization of hydrophobic substrates in Escherichia coli

scientific article

Picoliter nDEP traps enable time-resolved contactless single bacterial cell analysis in controlled microenvironments.

scientific article published in February 2013

Process and catalyst design objectives for specific redox biocatalysis.

scientific article published on January 2006

Process boundaries of irreversible scCO2-assisted phase separation in biphasic whole-cell biocatalysis

scientific article published on 30 June 2015

Production host selection for asymmetric styrene epoxidation: Escherichia coli vs. solvent-tolerant Pseudomonas.

scientific article

Proline availability regulates proline-4-hydroxylase synthesis and substrate uptake in proline-hydroxylating recombinant Escherichia coli.

scientific article

Purification and Characterization of 2-Hydroxybiphenyl 3-Monooxygenase, a Novel NADH-dependent, FAD-containing Aromatic Hydroxylase from Pseudomonas azelaica HBP1 ⬇️

scientific article published on September 26, 1997

Quantification of metabolic limitations during recombinant protein production in Escherichia coli.

scientific article

Quantitative physiology of Pichia pastoris during glucose-limited high-cell density fed-batch cultivation for recombinant protein production

scientific article published in October 2010

Reaction and catalyst engineering to exploit kinetically controlled whole-cell multistep biocatalysis for terminal FAME oxyfunctionalization.

scientific article

Real-time solvent tolerance analysis of pseudomonas sp. strain VLB120{Delta}C catalytic biofilms

scientific article published on 30 December 2010

Regioselective aromatic hydroxylation of quinaldine by water using quinaldine 4-oxidase in recombinant Pseudomonas putida

scientific article published on 02 October 2010

Research update for articles published in EJCI in 2012

Response of Pseudomonas putida KT2440 to increased NADH and ATP demand.

scientific article published on 29 July 2011

Resting cells of recombinant E. coli show high epoxidation yields on energy source and high sensitivity to product inhibition.

scientific article

Segmented flow is controlling growth of catalytic biofilms in continuous multiphase microreactors.

scientific article

Selected Pseudomonas putida strains able to grow in the presence of high butanol concentrations

scientific article published in May 2009

Simple enzymatic procedure for L-carnosine synthesis: whole-cell biocatalysis and efficient biocatalyst recycling

scientific article published on 4 August 2009

Single cell analysis reveals unexpected growth phenotype of S. cerevisiae

scientific article published in February 2009

Single cell analytics: an overview

scientific article published on January 2011

Single-cell analysis in biotechnology, systems biology, and biocatalysis

scientific article published on 08 March 2012

Solid support membrane-aerated catalytic biofilm reactor for the continuous synthesis of (S)-styrene oxide at gram scale

scientific article published on 08 September 2014

Stabilization of single species Synechocystis biofilms by cultivation under segmented flow

scientific article published on 7 May 2015

Steroid biotransformations in biphasic systems with Yarrowia lipolytica expressing human liver cytochrome P450 genes.

scientific article published on 9 August 2012

Subpopulation-proteomics in prokaryotic populations

scientific article

Subtoxic product levels limit the epoxidation capacity of recombinant E. coli by increasing microbial energy demands

scientific article published on 16 August 2012

TADH, the thermostable alcohol dehydrogenase from Thermus sp. ATN1: a versatile new biocatalyst for organic synthesis

scientific article published on 13 August 2008

Technical bias of microcultivation environments on single-cell physiology

scientific article published on April 2015

The Envirostat - a new bioreactor concept

scientific article published on 19 November 2008

The MOX promoter in Hansenula polymorpha is ultrasensitive to glucose-mediated carbon catabolite repression

scientific article published on 14 August 2016

The dynamic influence of cells on the formation of stable emulsions in organic-aqueous biotransformations.

scientific article published on 28 April 2015

The efficiency of recombinant Escherichia coli as biocatalyst for stereospecific epoxidation.

scientific article

The functional structure of central carbon metabolism in Pseudomonas putida KT2440.

scientific article published on 20 June 2014

The glycerophospholipid inventory of Pseudomonas putida is conserved between strains and enables growth condition-related alterations

scientific article published on 6 September 2011

The microbial cell-functional unit for energy dependent multistep biocatalysis

scientific article published on 16 July 2014

Towards a Biocatalyst for ( S )-Styrene Oxide Production: Characterization of the Styrene Degradation Pathway of Pseudomonas sp. Strain VLB120 ⬇️

scientific article published on June 1, 1998

Towards real time analysis of protein secretion from single cells

scientific article published on 21 September 2009

Trophic regulation of autoaggregation in Pseudomonas taiwanensis VLB120.

scientific article published in October 2015

Variability in subpopulation formation propagates into biocatalytic variability of engineered Pseudomonas putida strains.

scientific article

Whole-cell biocatalysis for selective and productive C-O functional group introduction and modification.

scientific article published on August 2013

Whole-cell-based CYP153A6-catalyzed (S)-limonene hydroxylation efficiency depends on host background and profits from monoterpene uptake via AlkL.

scientific article published on 9 February 2013

Δ(9)-Tetrahydrocannabinolic acid synthase production in Pichia pastoris enables chemical synthesis of cannabinoids

scientific article

Δ9-Tetrahydrocannabinolic acid synthase: The application of a plant secondary metabolite enzyme in biocatalytic chemical synthesis

scientific article published on 28 June 2016

List of works by Andreas Schmid

A rapid, reliable, and automatable lab-on-a-chip interface

A three-step method for analysing bacterial biofilm formation under continuous medium flow

An artificial TCA cycle selects for efficient α-ketoglutarate dependent hydroxylase catalysis in engineered Escherichia coli

Analysis of carbon and nitrogen co-metabolism in yeast by ultrahigh-resolution mass spectrometry applying 13C- and 15N-labeled substrates simultaneously

Analytical biotechnology: from single molecule and single cell analyses to population dynamics of metabolites and cells

Biofilms as living catalysts in continuous chemical syntheses

Carbon metabolism and product inhibition determine the epoxidation efficiency of solvent-tolerant Pseudomonas sp. strain VLB120DeltaC.

Carbon metabolism limits recombinant protein production in Pichia pastoris

Cell physiology rather than enzyme kinetics can determine the efficiency of cytochrome P450-catalyzed C-H-oxyfunctionalization

Challenging biological limits with microfluidic single cell analysis

Characterization of a biofilm membrane reactor and its prospects for fine chemical synthesis

Chemical and biological single cell analysis

Comparison of microbial hosts and expression systems for mammalian CYP1A1 catalysis

Complete genome sequence of Pseudomonas sp. strain VLB120 a solvent tolerant, styrene degrading bacterium, isolated from forest soil

Constitutively solvent-tolerant Pseudomonas taiwanensis VLB120∆ C∆ ttgV supports particularly high-styrene epoxidation activities when grown under glucose excess conditions

Continuous cyclohexane oxidation to cyclohexanol using a novel cytochrome P450 monooxygenase from Acidovorax sp. CHX100 in recombinant P. taiwanensis VLB120 biofilms

Continuous multistep synthesis of perillic acid from limonene by catalytic biofilms under segmented flow

Coupling limonene formation and oxyfunctionalization by mixed-culture resting cell fermentation.

D-Xylose assimilation via the Weimberg pathway by solvent-tolerant Pseudomonas taiwanensis VLB120.

Decoupling production from growth by magnesium sulfate limitation boosts de novo limonene production

Detection of volatile metabolites of Escherichia coli by multi capillary column coupled ion mobility spectrometry

Efficient hydroxyproline production from glucose in minimal media by Corynebacterium glutamicum.

Efficient phase separation and product recovery in organic-aqueous bioprocessing using supercritical carbon dioxide

Efficient production of the Nylon 12 monomer ω-aminododecanoic acid methyl ester from renewable dodecanoic acid methyl ester with engineered Escherichia coli.

Enantioselective substrate binding in a monooxygenase protein model by molecular dynamics and docking.

Engineered catalytic biofilms for continuous large scale production of n-octanol and (S)-styrene oxide

Engineering of Pseudomonas taiwanensis VLB120 for constitutive solvent tolerance and increased specific styrene epoxidation activity

Engineering the productivity of recombinant Escherichia coli for limonene formation from glycerol in minimal media.

Enzyme catalysis in an aqueous/organic segment flow microreactor: ways to stabilize enzyme activity

Glycerophospholipid profiling by high-performance liquid chromatography/mass spectrometry using exact mass measurements and multi-stage mass spectrometric fragmentation experiments in parallel

Guidelines for reporting of biocatalytic reactions

Guiding bioprocess design by microbial ecology

Guiding efficient microbial synthesis of non-natural chemicals by physicochemical properties of reactants

Heme-iron oxygenases: powerful industrial biocatalysts?

Hsp90 regulates the dynamics of its cochaperone Sti1 and the transfer of Hsp70 between modules

Hydrolase BioH knockout in E. coli enables efficient fatty acid methyl ester bioprocessing.

Integrated one-pot enrichment and immobilization of styrene monooxygenase (StyA) using SEPABEAD EC-EA and EC-Q1A anion-exchange carriers.

Integrated organic–aqueous biocatalysis and product recovery for quinaldine hydroxylation catalyzed by living recombinant Pseudomonas putida

Isolated microbial single cells and resulting micropopulations grow faster in controlled environments

Making variability less variable: matching expression system and host for oxygenase-based biotransformations.

Maximization of cell viability rather than biocatalyst activity improves whole-cell ω-oxyfunctionalization performance.

Maximizing the productivity of catalytic biofilms on solid supports in membrane aerated reactors

Metabolic and transcriptional response to cofactor perturbations in Escherichia coli.

Metabolic capacity estimation of Escherichia coli as a platform for redox biocatalysis: constraint-based modeling and experimental verification.

Metabolic engineering of Pseudomonas sp. strain VLB120 as platform biocatalyst for the production of isobutyric acid and other secondary metabolites

Metabolic flux analysis of a phenol producing mutant of Pseudomonas putida S12: verification and complementation of hypotheses derived from transcriptomics.

Metabolic network capacity of Escherichia coli for Krebs cycle-dependent proline hydroxylation.

Metabolic response of Pseudomonas putida during redox biocatalysis in the presence of a second octanol phase.

Microbial biofilms: a concept for industrial catalysis?

Microbial biofilms: new catalysts for maximizing productivity of long-term biotransformations

Microfluidic single-cell analysis links boundary environments and individual microbial phenotypes

Monitoring and control of microbioreactors: an expert opinion on development needs

NADH availability limits asymmetric biocatalytic epoxidation in a growing recombinant Escherichia coli strain.

Novel cyclohexane monooxygenase from Acidovorax sp. CHX100.

Outer membrane protein AlkL boosts biocatalytic oxyfunctionalization of hydrophobic substrates in Escherichia coli

Picoliter nDEP traps enable time-resolved contactless single bacterial cell analysis in controlled microenvironments.

Process and catalyst design objectives for specific redox biocatalysis.

Process boundaries of irreversible scCO2-assisted phase separation in biphasic whole-cell biocatalysis

Production host selection for asymmetric styrene epoxidation: Escherichia coli vs. solvent-tolerant Pseudomonas.

Proline availability regulates proline-4-hydroxylase synthesis and substrate uptake in proline-hydroxylating recombinant Escherichia coli.

Purification and Characterization of 2-Hydroxybiphenyl 3-Monooxygenase, a Novel NADH-dependent, FAD-containing Aromatic Hydroxylase from Pseudomonas azelaica HBP1 ⬇️

Quantification of metabolic limitations during recombinant protein production in Escherichia coli.

Quantitative physiology of Pichia pastoris during glucose-limited high-cell density fed-batch cultivation for recombinant protein production

Reaction and catalyst engineering to exploit kinetically controlled whole-cell multistep biocatalysis for terminal FAME oxyfunctionalization.

Real-time solvent tolerance analysis of pseudomonas sp. strain VLB120{Delta}C catalytic biofilms

Regioselective aromatic hydroxylation of quinaldine by water using quinaldine 4-oxidase in recombinant Pseudomonas putida

Research update for articles published in EJCI in 2012

Response of Pseudomonas putida KT2440 to increased NADH and ATP demand.

Resting cells of recombinant E. coli show high epoxidation yields on energy source and high sensitivity to product inhibition.

Segmented flow is controlling growth of catalytic biofilms in continuous multiphase microreactors.

Selected Pseudomonas putida strains able to grow in the presence of high butanol concentrations

Simple enzymatic procedure for L-carnosine synthesis: whole-cell biocatalysis and efficient biocatalyst recycling

Single cell analysis reveals unexpected growth phenotype of S. cerevisiae

Single cell analytics: an overview

Single-cell analysis in biotechnology, systems biology, and biocatalysis

Solid support membrane-aerated catalytic biofilm reactor for the continuous synthesis of (S)-styrene oxide at gram scale

Stabilization of single species Synechocystis biofilms by cultivation under segmented flow

Steroid biotransformations in biphasic systems with Yarrowia lipolytica expressing human liver cytochrome P450 genes.

Subpopulation-proteomics in prokaryotic populations