List of works - Richard J. Clark

A novel plant protein-disulfide isomerase involved in the oxidative folding of cystine knot defense proteins

scientific article published on 22 May 2007

A synthetic combinatorial strategy for developing alpha-conotoxin analogs as potent alpha7 nicotinic acetylcholine receptor antagonists

scientific article

Alanine scan of α-conotoxin RegIIA reveals a selective α3β4 nicotinic acetylcholine receptor antagonist.

scientific article

Analgesic alpha-conotoxins Vc1.1 and Rg1A inhibit N-type calcium channels in rat sensory neurons via GABAB receptor activation.

scientific article

Antifungal alkyl amino alcohols from the tropical marine sponge Haliclona n. sp.

scientific article published on December 2001

Are 9 10 Nicotinic Acetylcholine Receptors a Pain Target for -Conotoxins?

scientific article published on 05 September 2007

Beta-arrestin 2 is required for complement C1q expression in macrophages and constrains factor-independent survival

scientific article published on 26 September 2009

Binding mode of alpha-conotoxins to an acetylcholine binding protein determined by saturation transfer difference NMR.

scientific article

C5aR2 Activation Broadly Modulates the Signaling and Function of Primary Human Macrophages

scientific article published on 01 July 2020

Conserved structural and sequence elements implicated in the processing of gene-encoded circular proteins

scientific article

Cyclization of conotoxins to improve their biopharmaceutical properties.

scientific article published on 10 December 2010

Defining the Familial Fold of the Vicilin-Buried Peptide Family

scientific article published on 30 September 2020

Design, synthesis, and characterization of a single-chain peptide antagonist for the relaxin-3 receptor RXFP3.

scientific article published on 8 March 2011

Design, synthesis, and characterization of cyclic analogues of the iron regulatory peptide hormone hepcidin

scientific article published on September 2013

Development and validation of a LC-MS/MS assay for pharmacokinetic studies of complement C5a receptor antagonists PMX53 and PMX205 in mice.

scientific article

Differential Cav2.1 and Cav2.3 channel inhibition by baclofen and α-conotoxin Vc1.1 via GABAB receptor activation

scientific article published on April 2014

Discovery of functionally selective C5aR2 ligands: novel modulators of C5a signalling.

scientific article

Discovery of peptide probes to modulate oxytocin-type receptors of insects

scientific article published in Scientific Reports

Dissecting the oxidative folding of circular cystine knot miniproteins

scientific article published in May 2009

Disulfide bond mutagenesis and the structure and function of the head-to-tail macrocyclic trypsin inhibitor SFTI-1.

scientific article published in February 2005

Effects of cyclization on stability, structure, and activity of α-conotoxin RgIA at the α9α10 nicotinic acetylcholine receptor and GABA(B) receptor

scientific article published on 15 September 2011

Effects of linker sequence modifications on the structure, stability, and biological activity of a cyclic α-conotoxin

scientific article published on 01 November 2016

Engineering Cyclic Peptide Toxins

scientific article published on 01 January 2012

Engineering of conotoxins for the treatment of pain

scientific article published on December 2011

Engineering pro-angiogenic peptides using stable, disulfide-rich cyclic scaffolds

scientific article published on 28 October 2011

Engineering stabilized vascular endothelial growth factor-A antagonists: synthesis, structural characterization, and bioactivity of grafted analogues of cyclotides

scientific article published in December 2008

Engineering stable peptide toxins by means of backbone cyclization: stabilization of the alpha-conotoxin MII.

scientific article

Evaluation of toxicity and antitumor activity of cycloviolacin O2 in mice

scientific article published on January 2010

Exploring the Use of Helicogenic Amino Acids for Optimising Single Chain Relaxin-3 Peptide Agonists

scientific article published on 14 October 2020

G-Protein Coupled Receptors Targeted by Analgesic Venom Peptides

scientific article published on 16 November 2017

Helminth Immunomodulation in Autoimmune Disease

scientific article

Identifying key amino acid residues that affect α-conotoxin AuIB inhibition of α3β4 nicotinic acetylcholine receptors.

scientific article published on 07 October 2013

In vivo efficacy of anuran trypsin inhibitory peptides against staphylococcal skin infection and the impact of peptide cyclization

scientific article

Inhibition of Human Prolyl Oligopeptidase Activity by the Cyclotide Psysol 2 Isolated from Psychotria solitudinum

scientific article published on 20 April 2015

Inhibition of neuronal nicotinic acetylcholine receptor subtypes by alpha-Conotoxin GID and analogues

scientific article

Interlocking disulfides in circular proteins: toward efficient oxidative folding of cyclotides

scientific article published on 23 August 2010

Isolation, Characterization, and Synthesis of the Barrettides: Disulfide-Containing Peptides from the Marine Sponge Geodia barretti.

scientific article

Isolation, sequencing, and structure-activity relationships of cyclotides.

scientific article published on September 2010

Kalata B8, a novel antiviral circular protein, exhibits conformational flexibility in the cystine knot motif

scientific article published on February 2006

Microcin J25 has a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone

scientific article

Molecular Engineering of Conus Peptides as Therapeutic Leads

scientific article published in January 2017

Native chemical ligation applied to the synthesis and bioengineering of circular peptides and proteins

scientific article published on January 2010

Novel inhibitor cystine knot peptides from Momordica charantia

scientific article published in 2013

Peptide mimic for influenza vaccination using nonnatural combinatorial chemistry.

scientific article published on 12 March 2018

Periplasmic Expression of 4/7 α-Conotoxin TxIA Analogs in E. coli Favors Ribbon Isomer Formation - Suggestion of a Binding Mode at the α7 nAChR

scientific article published on 31 May 2019

Potential therapeutic applications of the cyclotides and related cystine knot mini-proteins

scientific article published on May 2007

Rational design of alpha-conotoxin analogues targeting alpha7 nicotinic acetylcholine receptors: improved antagonistic activity by incorporation of proline derivatives

scientific article

Release of bioactive peptides from polyurethane films in vitro and in vivo: Effect of polymer composition.

scientific article

Scanning mutagenesis of alpha-conotoxin Vc1.1 reveals residues crucial for activity at the alpha9alpha10 nicotinic acetylcholine receptor

scientific article published on 15 May 2009

Stabilization of α-Conotoxin AuIB: Influences of Disulfide Connectivity and Backbone Cyclization

scientific article published on 20 August 2010

Structural and functional analysis of human liver-expressed antimicrobial peptide 2.

scientific article published in October 2010

Structural plasticity of the cyclic-cystine-knot framework: implications for biological activity and drug design.

scientific article published in February 2006

Structure and activity of alpha-conotoxin PeIA at nicotinic acetylcholine receptor subtypes and GABA(B) receptor-coupled N-type calcium channels

scientific article

Structure of α-conotoxin BuIA: influences of disulfide connectivity on structural dynamics ⬇️

scientific article

Structure-Activity Studies of Cysteine-Rich α-Conotoxins that Inhibit High-Voltage-Activated Calcium Channels via GABA(B) Receptor Activation Reveal a Minimal Functional Motif.

scientific article

Structure–Activity Studies Reveal the Molecular Basis for GABAB-Receptor Mediated Inhibition of High Voltage-Activated Calcium Channels by α-Conotoxin Vc1.1

scientific article published on 25 May 2018

Sunflower trypsin inhibitor-1, proteolytic studies on a trypsin inhibitor peptide and its analogs.

scientific article

Synthetic hookworm-derived peptides are potent modulators of primary human immune cell function that protect against experimental colitis in vivo

scientific article published in 2021

The Engineering of an Orally Active Conotoxin for the Treatment of Neuropathic Pain

scientific article published on 01 September 2010

The N-terminal pro-domain of the kalata B1 cyclotide precursor is intrinsically unstructured

scientific article published on 26 August 2016

The absolute structural requirement for a proline in the P3'-position of Bowman-Birk protease inhibitors is surmounted in the minimized SFTI-1 scaffold

scientific article published on 09 June 2006

The alpine violet, Viola biflora, is a rich source of cyclotides with potent cytotoxicity

scientific article

The cyclotide fingerprint in oldenlandia affinis: elucidation of chemically modified, linear and novel macrocyclic peptides

scientific article published on 01 June 2007

The role of conserved Glu residue on cyclotide stability and activity: a structural and functional study of kalata B12, a naturally occurring Glu to Asp mutant

scientific article

The synthesis, structural characterization, and receptor specificity of the alpha-conotoxin Vc1.1.

scientific article published on 5 June 2006

The three-dimensional structure of the analgesic alpha-conotoxin, RgIA.

scientific article

Tick holocyclotoxins trigger host paralysis by presynaptic inhibition

scientific article published on 08 July 2016

Transcriptome and toxin family analysis of the paralysis tick, Ixodes holocyclus

scientific article

Transforming conotoxins into cyclotides: Backbone cyclization of P-superfamily conotoxins

scientific article

Ultra-stable peptide scaffolds for protein engineering-synthesis and folding of the circular cystine knotted cyclotide cycloviolacin O2.

scientific article published in January 2008

Understanding the structure/activity relationships of the iron regulatory peptide hepcidin

scientific article

Unveiling the diversity of cyclotides by combining peptidome and transcriptome analysis

scientific article published on 22 April 2016

List of works by Richard J. Clark

A novel plant protein-disulfide isomerase involved in the oxidative folding of cystine knot defense proteins

A synthetic combinatorial strategy for developing alpha-conotoxin analogs as potent alpha7 nicotinic acetylcholine receptor antagonists

Alanine scan of α-conotoxin RegIIA reveals a selective α3β4 nicotinic acetylcholine receptor antagonist.

Analgesic alpha-conotoxins Vc1.1 and Rg1A inhibit N-type calcium channels in rat sensory neurons via GABAB receptor activation.

Antifungal alkyl amino alcohols from the tropical marine sponge Haliclona n. sp.

Are 9 10 Nicotinic Acetylcholine Receptors a Pain Target for -Conotoxins?

Beta-arrestin 2 is required for complement C1q expression in macrophages and constrains factor-independent survival

Binding mode of alpha-conotoxins to an acetylcholine binding protein determined by saturation transfer difference NMR.

C5aR2 Activation Broadly Modulates the Signaling and Function of Primary Human Macrophages

Conserved structural and sequence elements implicated in the processing of gene-encoded circular proteins

Cyclization of conotoxins to improve their biopharmaceutical properties.

Defining the Familial Fold of the Vicilin-Buried Peptide Family

Design, synthesis, and characterization of a single-chain peptide antagonist for the relaxin-3 receptor RXFP3.

Design, synthesis, and characterization of cyclic analogues of the iron regulatory peptide hormone hepcidin

Development and validation of a LC-MS/MS assay for pharmacokinetic studies of complement C5a receptor antagonists PMX53 and PMX205 in mice.

Differential Cav2.1 and Cav2.3 channel inhibition by baclofen and α-conotoxin Vc1.1 via GABAB receptor activation

Discovery of functionally selective C5aR2 ligands: novel modulators of C5a signalling.

Discovery of peptide probes to modulate oxytocin-type receptors of insects

Dissecting the oxidative folding of circular cystine knot miniproteins

Disulfide bond mutagenesis and the structure and function of the head-to-tail macrocyclic trypsin inhibitor SFTI-1.

Effects of cyclization on stability, structure, and activity of α-conotoxin RgIA at the α9α10 nicotinic acetylcholine receptor and GABA(B) receptor

Effects of linker sequence modifications on the structure, stability, and biological activity of a cyclic α-conotoxin

Engineering Cyclic Peptide Toxins

Engineering of conotoxins for the treatment of pain

Engineering pro-angiogenic peptides using stable, disulfide-rich cyclic scaffolds

Engineering stabilized vascular endothelial growth factor-A antagonists: synthesis, structural characterization, and bioactivity of grafted analogues of cyclotides

Engineering stable peptide toxins by means of backbone cyclization: stabilization of the alpha-conotoxin MII.

Evaluation of toxicity and antitumor activity of cycloviolacin O2 in mice

Exploring the Use of Helicogenic Amino Acids for Optimising Single Chain Relaxin-3 Peptide Agonists

G-Protein Coupled Receptors Targeted by Analgesic Venom Peptides

Helminth Immunomodulation in Autoimmune Disease

Identifying key amino acid residues that affect α-conotoxin AuIB inhibition of α3β4 nicotinic acetylcholine receptors.

In vivo efficacy of anuran trypsin inhibitory peptides against staphylococcal skin infection and the impact of peptide cyclization

Inhibition of Human Prolyl Oligopeptidase Activity by the Cyclotide Psysol 2 Isolated from Psychotria solitudinum

Inhibition of neuronal nicotinic acetylcholine receptor subtypes by alpha-Conotoxin GID and analogues

Interlocking disulfides in circular proteins: toward efficient oxidative folding of cyclotides

Isolation, Characterization, and Synthesis of the Barrettides: Disulfide-Containing Peptides from the Marine Sponge Geodia barretti.

Isolation, sequencing, and structure-activity relationships of cyclotides.

Kalata B8, a novel antiviral circular protein, exhibits conformational flexibility in the cystine knot motif

Microcin J25 has a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone

Molecular Engineering of Conus Peptides as Therapeutic Leads

Native chemical ligation applied to the synthesis and bioengineering of circular peptides and proteins

Novel inhibitor cystine knot peptides from Momordica charantia

Peptide mimic for influenza vaccination using nonnatural combinatorial chemistry.

Periplasmic Expression of 4/7 α-Conotoxin TxIA Analogs in E. coli Favors Ribbon Isomer Formation - Suggestion of a Binding Mode at the α7 nAChR

Potential therapeutic applications of the cyclotides and related cystine knot mini-proteins

Rational design of alpha-conotoxin analogues targeting alpha7 nicotinic acetylcholine receptors: improved antagonistic activity by incorporation of proline derivatives

Release of bioactive peptides from polyurethane films in vitro and in vivo: Effect of polymer composition.

Scanning mutagenesis of alpha-conotoxin Vc1.1 reveals residues crucial for activity at the alpha9alpha10 nicotinic acetylcholine receptor

Stabilization of α-Conotoxin AuIB: Influences of Disulfide Connectivity and Backbone Cyclization

Structural and functional analysis of human liver-expressed antimicrobial peptide 2.

Structural plasticity of the cyclic-cystine-knot framework: implications for biological activity and drug design.

Structure and activity of alpha-conotoxin PeIA at nicotinic acetylcholine receptor subtypes and GABA(B) receptor-coupled N-type calcium channels

Structure of α-conotoxin BuIA: influences of disulfide connectivity on structural dynamics ⬇️

Structure-Activity Studies of Cysteine-Rich α-Conotoxins that Inhibit High-Voltage-Activated Calcium Channels via GABA(B) Receptor Activation Reveal a Minimal Functional Motif.

Structure–Activity Studies Reveal the Molecular Basis for GABAB-Receptor Mediated Inhibition of High Voltage-Activated Calcium Channels by α-Conotoxin Vc1.1

Sunflower trypsin inhibitor-1, proteolytic studies on a trypsin inhibitor peptide and its analogs.

Synthetic hookworm-derived peptides are potent modulators of primary human immune cell function that protect against experimental colitis in vivo

The Engineering of an Orally Active Conotoxin for the Treatment of Neuropathic Pain

The N-terminal pro-domain of the kalata B1 cyclotide precursor is intrinsically unstructured

The absolute structural requirement for a proline in the P3'-position of Bowman-Birk protease inhibitors is surmounted in the minimized SFTI-1 scaffold

The alpine violet, Viola biflora, is a rich source of cyclotides with potent cytotoxicity

The cyclotide fingerprint in oldenlandia affinis: elucidation of chemically modified, linear and novel macrocyclic peptides

The role of conserved Glu residue on cyclotide stability and activity: a structural and functional study of kalata B12, a naturally occurring Glu to Asp mutant

The synthesis, structural characterization, and receptor specificity of the alpha-conotoxin Vc1.1.

The three-dimensional structure of the analgesic alpha-conotoxin, RgIA.

Tick holocyclotoxins trigger host paralysis by presynaptic inhibition

Transcriptome and toxin family analysis of the paralysis tick, Ixodes holocyclus

Transforming conotoxins into cyclotides: Backbone cyclization of P-superfamily conotoxins

Ultra-stable peptide scaffolds for protein engineering-synthesis and folding of the circular cystine knotted cyclotide cycloviolacin O2.

Understanding the structure/activity relationships of the iron regulatory peptide hepcidin

Unveiling the diversity of cyclotides by combining peptidome and transcriptome analysis