List of works by Fernando de la Cruz

A bacterial TrwC relaxase domain contains a thermally stable alpha-helical core.

scientific article published on July 2003

A bacterial conjugation machinery recruited for pathogenesis

scientific article

A classification scheme for mobilization regions of bacterial plasmids.

scientific article published on February 2004

A degenerate primer MOB typing (DPMT) method to classify gamma-proteobacterial plasmids in clinical and environmental settings

scientific article

A high security double lock and key mechanism in HUH relaxases controls oriT-processing for plasmid conjugation

scientific article

A new domain of conjugative relaxase TrwC responsible for efficient oriT-specific recombination on minimal target sequences

scientific article published on 13 October 2006

ATPase activity and oligomeric state of TrwK, the VirB4 homologue of the plasmid R388 type IV secretion system

scientific article published on 6 June 2008

AcCNET (Accessory Genome Constellation Network): comparative genomics software for accessory genome analysis using bipartite networks.

scientific article published on 23 September 2016

Analysis of ColE1 MbeC unveils an extended ribbon-helix-helix family of nicking accessory proteins.

scientific article published on 29 December 2008

Analysis of DNA processing reactions in bacterial conjugation by using suicide oligonucleotides.

scientific article published on 26 July 2007

ArdC, a ssDNA-binding protein with a metalloprotease domain, overpasses the recipient hsdRMS restriction system broadening conjugation host range

scientific article published on 29 April 2020

Autoinhibitory regulation of TrwK, an essential VirB4 ATPase in type IV secretion systems

scientific article published on 24 March 2011

Bacterial computing with engineered populations

scientific article published on July 2015

Biochemical interactions between LPS and LPS-binding molecules

scientific article published on 13 January 2020

Blueprint for a minimal photoautotrophic cell: conserved and variable genes in Synechococcus elongatus PCC 7942.

scientific article

Breaking and joining single-stranded DNA: the HUH endonuclease superfamily

scientific article

CRISPR-Cas systems preferentially target the leading regions of MOBF conjugative plasmids.

scientific article published on 27 March 2013

Carriage of Extended-Spectrum Beta-Lactamase-Plasmids Does Not Reduce Fitness but Enhances Virulence in Some Strains of Pandemic E. coli Lineages.

scientific article

Catalytic domain of plasmid pAD1 relaxase TraX defines a group of relaxases related to restriction endonucleases

scientific article published on 31 July 2013

Changing the recognition site of a conjugative relaxase by rational design.

scientific article

Characterization of ATP and DNA Binding Activities of TrwB, the Coupling Protein Essential in Plasmid R388 Conjugation

scientific article published in Journal of Biological Chemistry

Characterization of the new insertion sequence IS91 from an alpha-hemolysin plasmid of Escherichia coli.

scientific article

Comparative Genomics of the Conjugation Region of F-like Plasmids: Five Shades of F.

scientific article published on 10 November 2016

Complementation of transposition of tnpA mutants of Tn3, Tn21, Tn501, and Tn1721

scientific article published on 01 November 1982

Concerted action of NIC relaxase and auxiliary protein MobC in RA3 plasmid conjugation.

scientific article published on 21 April 2016

Conjugation Inhibitors and Their Potential Use to Prevent Dissemination of Antibiotic Resistance Genes in Bacteria.

scientific article published on 30 November 2017

Conjugation inhibitors compete with palmitic acid for binding to the conjugative traffic ATPase TrwD, providing a mechanism to inhibit bacterial conjugation

scientific article published on 10 September 2018

Conjugative DNA metabolism in Gram-negative bacteria

scientific article published on January 2010

Conjugative plasmid protein TrwB, an integral membrane type IV secretion system coupling protein. Detailed structural features and mapping of the active site cleft

scientific article published on 17 December 2001

Conjugative transfer can be inhibited by blocking relaxase activity within recipient cells with intrabodies.

scientific article

Construction and properties of a family of pACYC184-derived cloning vectors compatible with pBR322 and its derivatives

scientific article published in June 1991

Construction of a family of Mycobacterium/Escherichia coli shuttle vectors derived from pAL5000 and pACYC184: their use for cloning an antibiotic-resistance gene from Mycobacterium fortuitum.

scientific article published on October 1996

Correlation between hepatitis B viremia and the clinical and histological activity of chronic delta hepatitis

scientific article published in July 1994

DNA binding properties of protein TrwA, a possible structural variant of the Arc repressor superfamily

scientific article published on 01 September 2004

Degenerate primer MOB typing of multiresistant clinical isolates of E. coli uncovers new plasmid backbones.

scientific article published on 22 November 2014

Deletion of a single helix from the transmembrane domain causes large changes in membrane insertion properties and secondary structure of the bacterial conjugation protein TrwB.

scientific article published on 25 August 2012

Design of Novel Relaxase Substrates Based on Rolling Circle Replicases for Bioconjugation to DNA Nanostructures

scientific article

Determination of conjugation rates on solid surfaces

scientific article published on 24 January 2012

Different pathways to acquiring resistance genes illustrated by the recent evolution of IncW plasmids.

scientific article

Dissemination of cephalosporin resistance genes between Escherichia coli strains from farm animals and humans by specific plasmid lineages

scientific article (publication date: December 2014)

Distribution of IS91 family insertion sequences in bacterial genomes: evolutionary implications

scientific article published in November 2002

Dynamical Task Switching in Cellular Computers

scientific article published on 26 January 2019

Dynamics of the IncW genetic backbone imply general trends in conjugative plasmid evolution.

scientific article published on 6 October 2006

Ecology and evolution as targets: the need for novel eco-evo drugs and strategies to fight antibiotic resistance

scientific article published on 16 May 2011

Engineering the fatty acid synthesis pathway in PCC 7942 improves omega-3 fatty acid production

scientific article published on 05 September 2018

Enzymology of type IV macromolecule secretion systems: the conjugative transfer regions of plasmids RP4 and R388 and the cag pathogenicity island of Helicobacter pylori encode structurally and functionally related nucleoside triphosphate hydrolases

scientific article published on May 2000

Escherichia coli alpha-haemolysin synthesis and export genes are flanked by a direct repetition of IS91-like elements

article

Escherichia coli genes affecting recipient ability in plasmid conjugation: are there any?

scientific article

Escherichia coli hha mutants, DNA supercoiling and expression of the haemolysin genes from the recombinant plasmid pANN202-312.

scientific article published on September 1993

Evolution of conjugation and type IV secretion systems

scientific article published on 13 September 2012

Factors that affect transposition mediated by the Tn21 transposase

scientific article published on 01 July 1988

Four main virotypes among extended-spectrum-β-lactamase-producing isolates of Escherichia coli O25b:H4-B2-ST131: bacterial, epidemiological, and clinical characteristics.

scientific article published on 07 August 2013

Functional dissection of the conjugative coupling protein TrwB.

scientific article published on 2 April 2010

Functional domains in protein TrwC of plasmid R388: dissected DNA strand transferase and DNA helicase activities reconstitute protein function.

scientific article

Functional interactions between type IV secretion systems involved in DNA transfer and virulence

scientific article published on 01 November 2005

Functional interactions of VirB11 traffic ATPases with VirB4 and VirD4 molecular motors in type IV secretion systems

scientific article published on 12 July 2013

Genetic and biochemical characterization of MbeA, the relaxase involved in plasmid ColE1 conjugative mobilization

scientific article published in April 2003

Genetic evidence of a coupling role for the TraG protein family in bacterial conjugation

scientific article published in April 1997

Genetic organization of the conjugal DNA processing region of the IncW plasmid R388.

scientific article published on January 1994

Genetics of the replication and maintenance functions of the hemolytic plasmid pSU316. Cloning of an IncFIII determinant

article

Genomic analysis of the emergence and evolution of multidrug resistance during a Klebsiella pneumoniae outbreak including carbapenem and colistin resistance.

scientific article published on 23 October 2013

Genomic and metagenomic technologies to explore the antibiotic resistance mobilome

scientific article published on 10 November 2016

Genomics of high molecular weight plasmids isolated from an on-farm biopurification system

scientific article published on 20 June 2016

Heterologous expression of a thermophilic diacylglycerol acyltransferase triggers triglyceride accumulation in Escherichia coli.

scientific article

Horizontal gene transfer and the origin of species: lessons from bacteria.

scientific article

Host Range and Genetic Plasticity Explain the Coexistence of Integrative and Extrachromosomal Mobile Genetic Elements

scientific article published on 01 November 2018

Host range and genetic plasticity explain the co-existence of integrative and extrachromosomal mobile genetic elements

scholarly article by Jean Cury published in June 2018

IS91 transposase is related to the rolling-circle-type replication proteins of the pUB110 family of plasmids

scientific article

Identification of Xenologs and Their Characteristic Low Expression Levels in the Cyanobacterium Synechococcus elongatus.

scientific article published on 4 June 2015

Identification of bacterial plasmids based on mobility and plasmid population biology.

scientific article published on 29 July 2011

In vivo transmission of a plasmid coharbouring bla and qnrB genes between Escherichia coli and Serratia marcescens.

scientific article

In-depth resistome analysis by targeted metagenomics.

scientific article

Incompatibility among alpha-hemolytic plasmids studied after inactivation of the alpha-hemolysin gene by transposition of Tn802.

scientific article published on October 1979

Interaction between relaxase MbeA and accessory protein MbeC of the conjugally mobilizable plasmid ColE1.

scientific article published on 14 February 2012

Intramolecular transposition of insertion sequence IS91 results in second-site simple insertions.

scientific article published in July 1999

Junction sequences generated by 'one-ended transposition'.

scientific article published on May 1985

Membrane insertion stabilizes the structure of TrwB, the R388 conjugative plasmid coupling protein.

scientific article published on 4 January 2011

Mobility of plasmids.

scientific article published on September 2010

Molecular epidemiology and virulence of Escherichia coli O16:H5-ST131: comparison with H30 and H30-Rx subclones of O25b:H4-ST131.

scientific article

Multicellular computing using conjugation for wiring

scientific article (publication date: 2013)

Nanobiotechnology

scientific article published on 07 June 2012

Natural and Artificial Strategies To Control the Conjugative Transmission of Plasmids.

scientific article published in January 2018

Negative feedback and transcriptional overshooting in a regulatory network for horizontal gene transfer.

scientific article

Negative feedback increases information transmission, enabling bacteria to discriminate sublethal antibiotic concentrations

article

Numbers on the edges: A simplified and scalable method for quantifying the Gene Regulation Function

article

Orthogonal Protein Assembly on DNA Nanostructures Using Relaxases

scientific article published on 24 February 2016

PLACNETw: a web-based tool for plasmid reconstruction from bacterial genomes

scientific article published on 21 July 2017

Physical and genetic map of the IncW plasmid R388

scientific article published on 01 September 1988

PifC and Osa, Plasmid Weapons against Rival Conjugative Coupling Proteins.

scientific article

PipX, the coactivator of NtcA, is a global regulator in cyanobacteria

scientific article

Plasmid Diversity and Adaptation Analyzed by Massive Sequencing of Escherichia coli Plasmids

scientific article published on December 2014

Plasmid R6K contains two functional oriTs which can assemble simultaneously in relaxosomes in vivo

scientific article

Plasmid flux in Escherichia coli ST131 sublineages, analyzed by plasmid constellation network (PLACNET), a new method for plasmid reconstruction from whole genome sequences.

scientific article

Plasmid r1 conjugative DNA processing is regulated at the coupling protein interface.

scientific article published on 18 September 2009

Plasmid typing and genetic context of AmpC β-lactamases in Enterobacteriaceae lacking inducible chromosomal ampC genes: findings from a Spanish hospital 1999-2007.

scientific article

Plasmids containing one inverted repeat of Tn21 can fuse with other plasmids in the presence of Tn21 transposase

scientific article published on 01 January 1984

Population genomics and antimicrobial resistance dynamics of Escherichia coli in wastewater and river environments

scientific article published on 12 April 2021

Purification and biochemical characterization of TrwC, the helicase involved in plasmid R388 conjugal DNA transfer.

scientific article

Purification and properties of TrwB, a hexameric, ATP-binding integral membrane protein essential for R388 plasmid conjugation

scientific article

Purification of α-hemolysin from an overproducing E. coli strain

scientific article published on 01 January 1985

Regulation of finP transcription by DNA adenine methylation in the virulence plasmid of Salmonella enterica

scientific article

Regulation of the type IV secretion ATPase TrwD by magnesium: implications for catalytic mechanism of the secretion ATPase superfamily.

scientific article

Relaxase DNA binding and cleavage are two distinguishable steps in conjugative DNA processing that involve different sequence elements of the nic site

scientific article published on 08 January 2010

Relaxases and Plasmid Transfer in Gram-Negative Bacteria.

scientific article published in January 2017

Release of lipid vesicle contents by the bacterial protein toxin alpha-haemolysin.

scientific article

Role of IncHI2 plasmids harbouring blaVIM-1, blaCTX-M-9, aac(6')-Ib and qnrA genes in the spread of multiresistant Enterobacter cloacae and Klebsiella pneumoniae strains in different units at Hospital Vall d'Hebron, Barcelona, Spain.

scientific article published on 4 April 2012

Role of the transmembrane domain in the stability of TrwB, an integral protein involved in bacterial conjugation

scientific article published on 29 December 2003

Single-stranded DNA intermediates in IS91 rolling-circle transposition

scientific article

Site-specific recombination and shuffling of resistance genes in transposon Tn21 ⬇️

scientific article published on July 1, 1991

Specificity of insertion of IS91, an insertion sequence present in alpha-haemolysin plasmids of Escherichia coli.

scientific article

Spread of bla(CTX-M-14) is driven mainly by IncK plasmids disseminated among Escherichia coli phylogroups A, B1, and D in Spain.

scientific article published on 28 September 2009

Structural and functional analysis of the origin of conjugal transfer of the broad-host-range IncW plasmid R388 and comparison with the related IncN plasmid R46.

scientific article published in May 1991

Structural independence of conjugative coupling protein TrwB from its Type IV secretion machinery

scientific article published on 10 April 2013

Structure and role of coupling proteins in conjugal DNA transfer.

scientific article

Subcellular location of the coupling protein TrwB and the role of its transmembrane domain

scientific article published on 6 September 2013

Substrate translocation involves specific lysine residues of the central channel of the conjugative coupling protein TrwB.

scientific article

Synthetic Fatty Acids Prevent Plasmid-Mediated Horizontal Gene Transfer

scientific article

Tanzawaic Acids, a Chemically Novel Set of Bacterial Conjugation Inhibitors

scientific article published on 26 January 2016

The ATPase activity of the DNA transporter TrwB is modulated by protein TrwA: implications for a common assembly mechanism of DNA translocating motors

scientific article published on 27 June 2007

The Hha protein from Escherichia coli is highly homologous to the YmoA protein from Yersinia enterocolitica

scientific article published on 01 November 1992

The bacterial conjugation protein TrwB resembles ring helicases and F1-ATPase

scientific article

The calcium-binding C-terminal domain of Escherichia coli alpha-hemolysin is a major determinant in the surface-active properties of the protein.

scientific article published on 26 February 2007

The conjugative DNA translocase TrwB is a structure-specific DNA-binding protein

scientific article published on 06 April 2010

The hexameric structure of a conjugative VirB4 protein ATPase provides new insights for a functional and phylogenetic relationship with DNA translocases

scientific article published on 3 October 2012

The hha gene modulates haemolysin expression in Escherichia coli

scientific article published on May 1991

The relaxase of the Rhizobium etli symbiotic plasmid shows nic site cis-acting preference.

scientific article published on 17 August 2006

The stb operon balances the requirements for vegetative stability and conjugative transfer of plasmid R388

scientific article

The transmembrane domain of the T4SS coupling protein TrwB and its role in protein-protein interactions

scientific article published on 2 June 2013

The transmembrane domain provides nucleotide binding specificity to the bacterial conjugation protein TrwB

scientific article published on 27 April 2006

Tn5tac1 insertion polarity in Escherichia coli

scientific article published on 01 November 1991

Toward minimal bacterial cells: evolution vs. design

scientific article published on 03 December 2008

Towards a taxonomy of conjugative plasmids.

scientific article published on 3 June 2017

Towards an integrated model of bacterial conjugation

scientific article

Transcription factor-based biosensors enlightened by the analyte

scientific article (publication date: 2015)

Transcription modulation of Salmonella enterica serovar Typhimurium promoters by sub-MIC levels of rifampin

scientific article

Transposon Tn21 encodes a RecA-independent site-specific integration system

scientific article published on 01 February 1988

TrwB: an F(1)-ATPase-like molecular motor involved in DNA transport during bacterial conjugation.

scientific article

TrwD, a protein encoded by the IncW plasmid R388, displays an ATP hydrolase activity essential for bacterial conjugation

scientific article published in October 1997

TrwD, the hexameric traffic ATPase encoded by plasmid R388, induces membrane destabilization and hemifusion of lipid vesicles

scientific article published on March 2002

Two active-site tyrosyl residues of protein TrwC act sequentially at the origin of transfer during plasmid R388 conjugation

scientific article published on February 2000

Two atypical mobilization proteins are involved in plasmid CloDF13 relaxation

scientific article published in February 2001

Type IV traffic ATPase TrwD as molecular target to inhibit bacterial conjugation

scientific article published on 24 February 2016

Unsaturated fatty acids are inhibitors of bacterial conjugation

scientific article

Unveiling the molecular mechanism of a conjugative relaxase: The structure of TrwC complexed with a 27-mer DNA comprising the recognition hairpin and the cleavage site.

scientific article published on 28 February 2006

Use of limited proteolysis and mutagenesis to identify folding domains and sequence motifs critical for wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase activity

scientific article published on 02 December 2013

Viral replication in patients with concomitant hepatitis B and C virus infections

scientific article published on June 1997

Whole genome sequencing, molecular typing and in vivo virulence of OXA-48-producing Escherichia coli isolates including ST131 H30-Rx, H22 and H41 subclones

scientific article published on 21 September 2017

Why is entry exclusion an essential feature of conjugative plasmids?

scientific article published on 28 April 2008

fabH deletion increases DHA production in Escherichia coli expressing Pfa genes.

scientific article

oriT-processing and regulatory roles of TrwA protein in plasmid R388 conjugation.

scientific article published in July 1997

pACYC184-derived cloning vectors containing the multiple cloning site and lacZ alpha reporter gene of pUC8/9 and pUC18/19 plasmids

scientific article published in August 1988

α-Haemolysin from E. coli purification and self-aggregation properties

scientific article published on 01 March 1991

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