List of works - Sandra Villegas

2D-NMR reveals different populations of exposed lysine residues in the apoB-100 protein of electronegative and electropositive fractions of LDL particles

scientific article published on 28 January 2010

A thermodynamic study of the third PDZ domain of MAGUK neuronal protein PSD-95 reveals a complex three-state folding behavior

scientific article published on 2 November 2013

Aggregated electronegative low density lipoprotein in human plasma shows a high tendency toward phospholipolysis and particle fusion

scientific article published on 29 July 2010

Alzheimer's disease: New therapeutic strategies

scholarly article by Sandra Villegas published July 2015 in Medicina Clínica (English Edition)

An Intracellular Amyloid-β/AβPP Epitope Correlates with Neurodegeneration in those Neuronal Populations Early Involved in Alzheimer's Disease

scientific article published on 4 July 2017

An anti-Aβ (amyloid β) single-chain variable fragment prevents amyloid fibril formation and cytotoxicity by withdrawing Aβ oligomers from the amyloid pathway

scientific article published on July 2011

Analysis of the activation process of porcine procarboxypeptidase B and determination of the sequence of its activation segment

scientific article published in April 1991

Apolipoprotein J mimetic peptide [113–122]apoj decreases weight gain in LDLR-KO mice under atherogenic diet by decreasing fat accumulation

Aβ immunotherapy for Alzheimer's disease: where are we?

scholarly article by Jofre Güell-Bosch et al published June 2016 in Neurodegenerative disease management

Aβ-Immunotherapeutic strategies: a wide range of approaches for Alzheimer's disease treatment

scientific article published on 30 June 2016

Aβ-oligomer uptake and the resulting inflammatory response in adult human astrocytes are precluded by an anti-Aβ single chain variable fragment in combination with an apoE mimetic peptide.

scientific article published on 3 April 2018

Both Amyloid-β Peptide and Tau Protein Are Affected by an Anti-Amyloid-β Antibody Fragment in Elderly 3xTg-AD Mice

scientific article published on 10 September 2020

Cerebellar cortex development in the weaver condition presents regional and age-dependent abnormalities without differences in Purkinje cells neurogenesis

scientific article

Clusterin/apolipoprotein J binds to aggregated LDL in human plasma and plays a protective role against LDL aggregation.

scientific article published on 30 December 2014

Cognitive Impairment in the 3xTg-AD Mouse Model of Alzheimer's Disease is Affected by Aβ-ImmunoTherapy and Cognitive Stimulation

scientific article published on 02 October 2020

Common features in the unfolding and misfolding of PDZ domains and beyond: the modulatory effect of domain swapping and extra-elements

scientific article published on 12 January 2016

Comparative analysis of the sequences and three-dimensional models of human procarboxypeptidases A1, A2 and B.

scientific article published on February 1998

Conformational and functional variants of CD44-targeted protein nanoparticles bio-produced in bacteria

scientific article published on 14 April 2016

Differential effects of apoE and apoJ mimetic peptides on the action of an anti-Aβ scFv in 3xTg-AD mice

scientific article published on 17 July 2018

Early intervention in the 3xTg-AD mice with an amyloid β-antibody fragment ameliorates first hallmarks of Alzheimer disease.

scientific article published on 20 June 2013

Early kinetics of amyloid fibril formation reveals conformational reorganisation of initial aggregates

scientific article published on 15 December 2006

Effects of an Aβ-antibody fragment on Aβ aggregation and astrocytic uptake are modulated by apolipoprotein E and J mimetic peptides

scientific article published on 20 November 2017

Electronegative low-density lipoprotein. A link between apolipoprotein B misfolding, lipoprotein aggregation and proteoglycan binding.

scientific article published on October 2012

Elongation of the C-terminal domain of an anti-amyloid β single-chain variable fragment increases its thermodynamic stability and decreases its aggregation tendency

scientific article published on 19 June 2013

Enfermedad de Alzheimer: nuevas estrategias terapéuticas

Evidence for a two-state transition in the folding process of the activation domain of human procarboxypeptidase A2.

scientific article published on November 1995

Expression of heat-labile enterotoxin genes is under cyclic AMP control inEscherichia coli

article

Favourable native-like helical local interactions can accelerate protein folding

scientific article

Functional inclusion bodies produced in the yeast Pichia pastoris.

scientific article published on October 2016

High binding affinity of electronegative LDL to human aortic proteoglycans depends on its aggregation level

scientific article

Hydrogen exchange monitored by MALDI-TOF mass spectrometry for rapid characterization of the stability and conformation of proteins.

scientific article

Immunotherapy for neurodegenerative diseases: the Alzheimer's disease paradigm

scholarly article by Laia Montoliu-Gaya & Sandra Villegas published March 2018 in Current opinion in chemical engineering

Impaired binding affinity of electronegative low-density lipoprotein (LDL) to the LDL receptor is related to nonesterified fatty acids and lysophosphatidylcholine content

scientific article published in December 2004

Influence of aggregation propensity and stability on amyloid fibril formation as studied by Fourier transform infrared spectroscopy and two-dimensional COS analysis

scientific article published on November 2009

Loss of deep cerebellar nuclei neurons in the 3xTg-AD mice and protection by an anti-amyloid β antibody fragment

scientific article published on 20 June 2013

Mapping the pro-region of carboxypeptidase B by protein engineering. Cloning, overexpression, and mutagenesis of the porcine proenzyme

scientific article published in July 1999

Modified Forms of LDL in Plasma

Monitoring disappearance of monomers and generation of resistance to proteolysis during the formation of the activation domain of human procarboxypeptidase A2 (ADA2h) amyloid fibrils by matrix-assisted laser-desorption ionization-time-of-flight-MS.

scientific article

Mouse Models of Alzheimer's Disease

scientific article published on 10 March 2017

Mutations can cause light chains to be too stable or too unstable to form amyloid fibrils

scientific article published on 24 August 2015

NMR solution structure of the activation domain of human procarboxypeptidase A2

scientific article

Novel phospholipolytic activities associated with electronegative low-density lipoprotein are involved in increased self-aggregation.

scientific article published on 8 July 2008

Overexpression of Human Procarboxypeptidase A2 inPichia pastorisand Detailed Characterization of Its Activation Pathway

scientific article published in Journal of Biological Chemistry

Pharmacokinetic parameters and mechanism of action of an efficient anti-Aβ single chain antibody fragment

scientific article published on 31 May 2019

Prediction of a new class of RNA recognition motif

scientific article published on 17 November 2010

Principal component and cluster analysis of morphological variables reveals multiple discrete sub-phenotypes in weaver mouse mutants

scientific article published in June 2013

Production of an anti-Aβ antibody fragment in Pichia pastoris and in vitro and in vivo validation of its therapeutic effect.

scientific article

Progression of Alzheimer's disease and effect of scFv-h3D6 immunotherapy in the 3xTg-AD mouse model: An in vivo longitudinal study using Magnetic Resonance Imaging and Spectroscopy

scientific article published on 17 February 2020

Protein engineering as a strategy to avoid formation of amyloid fibrils

scientific article published on September 2000

Protein secondary structure and stability determined by combining exoproteolysis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

scientific article

Protein structures in Alzheimer's disease: The basis for rationale therapeutic design

scientific article published on 13 October 2015

Stabilization of proteins by rational design of alpha-helix stability using helix/coil transition theory

scientific article published in January 1996

Structure of the transition state in the folding process of human procarboxypeptidase A2 activation domain

scientific article published on 01 November 1998

The Role of Apolipoprotein E Isoforms in Alzheimer's Disease

scientific article published on 01 January 2019

The activation pathway of procarboxypeptidase B from porcine pancreas: participation of the active enzyme in the proteolytic processing

scientific article published on September 1995

The chondroitin sulfate/dermatan sulfate 4-O-endosulfatase from marine bacterium Vibrio sp FC509 is a dimeric species: Biophysical characterization of an endosulfatase

scientific article

The impact of extra-domain structures and post-translational modifications in the folding/misfolding behaviour of the third PDZ domain of MAGUK neuronal protein PSD-95.

scientific article published on 20 May 2014

The interconversion between a flexible β-sheet and a fibril β-arrangement constitutes the main conformational event during misfolding of PSD95-PDZ3 domain

scientific article

The isolated N terminus of Ring1B is a well-folded, monomeric fragment with native-like structure

The structure of human prokallikrein 6 reveals a novel activation mechanism for the kallikrein family

scientific article

The unfolding pathway of leech carboxypeptidase inhibitor

scientific article published on 13 March 2002

Thermodynamic analysis of helix-engineered forms of the activation domain of human procarboxypeptidase A2.

scientific article

Towards the improvement in stability of an anti-Aβ single-chain variable fragment, scFv-h3D6, as a way to enhance its therapeutic potential

scientific article

Understanding the contribution of disulfide bridges to the folding and misfolding of an anti-Aβ scFv

scientific article published on 24 March 2017

List of works by Sandra Villegas

2D-NMR reveals different populations of exposed lysine residues in the apoB-100 protein of electronegative and electropositive fractions of LDL particles

A thermodynamic study of the third PDZ domain of MAGUK neuronal protein PSD-95 reveals a complex three-state folding behavior

Aggregated electronegative low density lipoprotein in human plasma shows a high tendency toward phospholipolysis and particle fusion

Alzheimer's disease: New therapeutic strategies

An Intracellular Amyloid-β/AβPP Epitope Correlates with Neurodegeneration in those Neuronal Populations Early Involved in Alzheimer's Disease

An anti-Aβ (amyloid β) single-chain variable fragment prevents amyloid fibril formation and cytotoxicity by withdrawing Aβ oligomers from the amyloid pathway

Analysis of the activation process of porcine procarboxypeptidase B and determination of the sequence of its activation segment

Apolipoprotein J mimetic peptide [113–122]apoj decreases weight gain in LDLR-KO mice under atherogenic diet by decreasing fat accumulation

Aβ immunotherapy for Alzheimer's disease: where are we?

Aβ-Immunotherapeutic strategies: a wide range of approaches for Alzheimer's disease treatment

Aβ-oligomer uptake and the resulting inflammatory response in adult human astrocytes are precluded by an anti-Aβ single chain variable fragment in combination with an apoE mimetic peptide.

Both Amyloid-β Peptide and Tau Protein Are Affected by an Anti-Amyloid-β Antibody Fragment in Elderly 3xTg-AD Mice

Cerebellar cortex development in the weaver condition presents regional and age-dependent abnormalities without differences in Purkinje cells neurogenesis

Clusterin/apolipoprotein J binds to aggregated LDL in human plasma and plays a protective role against LDL aggregation.

Cognitive Impairment in the 3xTg-AD Mouse Model of Alzheimer's Disease is Affected by Aβ-ImmunoTherapy and Cognitive Stimulation

Common features in the unfolding and misfolding of PDZ domains and beyond: the modulatory effect of domain swapping and extra-elements

Comparative analysis of the sequences and three-dimensional models of human procarboxypeptidases A1, A2 and B.

Conformational and functional variants of CD44-targeted protein nanoparticles bio-produced in bacteria

Differential effects of apoE and apoJ mimetic peptides on the action of an anti-Aβ scFv in 3xTg-AD mice

Early intervention in the 3xTg-AD mice with an amyloid β-antibody fragment ameliorates first hallmarks of Alzheimer disease.

Early kinetics of amyloid fibril formation reveals conformational reorganisation of initial aggregates

Effects of an Aβ-antibody fragment on Aβ aggregation and astrocytic uptake are modulated by apolipoprotein E and J mimetic peptides

Electronegative low-density lipoprotein. A link between apolipoprotein B misfolding, lipoprotein aggregation and proteoglycan binding.

Elongation of the C-terminal domain of an anti-amyloid β single-chain variable fragment increases its thermodynamic stability and decreases its aggregation tendency

Enfermedad de Alzheimer: nuevas estrategias terapéuticas

Evidence for a two-state transition in the folding process of the activation domain of human procarboxypeptidase A2.

Expression of heat-labile enterotoxin genes is under cyclic AMP control inEscherichia coli

Favourable native-like helical local interactions can accelerate protein folding

Functional inclusion bodies produced in the yeast Pichia pastoris.

High binding affinity of electronegative LDL to human aortic proteoglycans depends on its aggregation level

Hydrogen exchange monitored by MALDI-TOF mass spectrometry for rapid characterization of the stability and conformation of proteins.

Immunotherapy for neurodegenerative diseases: the Alzheimer's disease paradigm

Impaired binding affinity of electronegative low-density lipoprotein (LDL) to the LDL receptor is related to nonesterified fatty acids and lysophosphatidylcholine content

Influence of aggregation propensity and stability on amyloid fibril formation as studied by Fourier transform infrared spectroscopy and two-dimensional COS analysis

Loss of deep cerebellar nuclei neurons in the 3xTg-AD mice and protection by an anti-amyloid β antibody fragment

Mapping the pro-region of carboxypeptidase B by protein engineering. Cloning, overexpression, and mutagenesis of the porcine proenzyme

Modified Forms of LDL in Plasma

Monitoring disappearance of monomers and generation of resistance to proteolysis during the formation of the activation domain of human procarboxypeptidase A2 (ADA2h) amyloid fibrils by matrix-assisted laser-desorption ionization-time-of-flight-MS.

Mouse Models of Alzheimer's Disease

Mutations can cause light chains to be too stable or too unstable to form amyloid fibrils

NMR solution structure of the activation domain of human procarboxypeptidase A2

Novel phospholipolytic activities associated with electronegative low-density lipoprotein are involved in increased self-aggregation.

Overexpression of Human Procarboxypeptidase A2 inPichia pastorisand Detailed Characterization of Its Activation Pathway

Pharmacokinetic parameters and mechanism of action of an efficient anti-Aβ single chain antibody fragment

Prediction of a new class of RNA recognition motif

Principal component and cluster analysis of morphological variables reveals multiple discrete sub-phenotypes in weaver mouse mutants

Production of an anti-Aβ antibody fragment in Pichia pastoris and in vitro and in vivo validation of its therapeutic effect.

Progression of Alzheimer's disease and effect of scFv-h3D6 immunotherapy in the 3xTg-AD mouse model: An in vivo longitudinal study using Magnetic Resonance Imaging and Spectroscopy

Protein engineering as a strategy to avoid formation of amyloid fibrils

Protein secondary structure and stability determined by combining exoproteolysis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Protein structures in Alzheimer's disease: The basis for rationale therapeutic design

Stabilization of proteins by rational design of alpha-helix stability using helix/coil transition theory

Structure of the transition state in the folding process of human procarboxypeptidase A2 activation domain

The Role of Apolipoprotein E Isoforms in Alzheimer's Disease

The activation pathway of procarboxypeptidase B from porcine pancreas: participation of the active enzyme in the proteolytic processing

The chondroitin sulfate/dermatan sulfate 4-O-endosulfatase from marine bacterium Vibrio sp FC509 is a dimeric species: Biophysical characterization of an endosulfatase

The impact of extra-domain structures and post-translational modifications in the folding/misfolding behaviour of the third PDZ domain of MAGUK neuronal protein PSD-95.

The interconversion between a flexible β-sheet and a fibril β-arrangement constitutes the main conformational event during misfolding of PSD95-PDZ3 domain

The isolated N terminus of Ring1B is a well-folded, monomeric fragment with native-like structure

The structure of human prokallikrein 6 reveals a novel activation mechanism for the kallikrein family

The unfolding pathway of leech carboxypeptidase inhibitor

Thermodynamic analysis of helix-engineered forms of the activation domain of human procarboxypeptidase A2.

Towards the improvement in stability of an anti-Aβ single-chain variable fragment, scFv-h3D6, as a way to enhance its therapeutic potential

Understanding the contribution of disulfide bridges to the folding and misfolding of an anti-Aβ scFv