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List of works by Alexander A. Khromykh

A Highly Structured, Nuclease-Resistant, Noncoding RNA Produced by Flaviviruses Is Required for Pathogenicity

scientific article

A Kunjin Replicon Virus-like Particle Vaccine Provides Protection Against Ebola Virus Infection in Nonhuman Primates

scientific article

A Kunjin replicon vector encoding granulocyte macrophage colony-stimulating factor for intra-tumoral gene therapy

scientific article published on 18 December 2008

A Single Amino Acid Substitution in the West Nile Virus Nonstructural Protein NS2A Disables Its Ability To Inhibit Alpha/Beta Interferon Induction and Attenuates Virus Virulence in Mice

scientific article

A noncoding RNA produced by arthropod-borne flaviviruses inhibits the cellular exoribonuclease XRN1 and alters host mRNA stability

scientific article

A novel bacterium-free method for generation of flavivirus infectious DNA by circular polymerase extension reaction allows accurate recapitulation of viral heterogeneity

scientific article published on 12 December 2012

A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses

scientific article published on 08 June 2021

An interaction between the methyltransferase and RNA dependent RNA polymerase domains of the West Nile virus NS5 protein

scientific article published on 5 June 2013

Analysis of adaptive mutations in Kunjin virus replicon RNA reveals a novel role for the flavivirus nonstructural protein NS2A in inhibition of beta interferon promoter-driven transcription

scientific article

Cell fusing agent virus and dengue virus mutually interact in Aedes aegypti cell lines.

scientific article published on 31 July 2017

Characterization of recombinant Flaviviridae viruses possessing a small reporter-tag

scientific article published in November 2017

Characterization of virulent West Nile virus Kunjin strain, Australia, 2011.

scientific article

Chikungunya virus nonstructural protein 2 inhibits type I/II interferon-stimulated JAK-STAT signaling

scientific article

Chimeric viruses between Rocio and West Nile: the role for Rocio prM-E proteins in virulence and inhibition of interferon-α/β signaling

scientific article published on 20 March 2017

Cholesterol manipulation by West Nile virus perturbs the cellular immune response

scientific article published on October 2007

Cloning and expression of the ataxia-telangiectasia gene in baculovirus

scientific article published on April 1998

Comparative mechanistic studies of de novo RNA synthesis by flavivirus RNA-dependent RNA polymerases.

scientific article

Comparison of attenuated and virulent West Nile virus strains in human monocyte-derived dendritic cells as a model of initial human infection

scientific article published on 22 March 2015

Complementation analysis of the flavivirus Kunjin NS3 and NS5 proteins defines the minimal regions essential for formation of a replication complex and shows a requirement of NS3 in cis for virus assembly

scientific article

Construction and biological characterization of artificial recombinants between a wild type flavivirus (Kunjin) and a live chimeric flavivirus vaccine (ChimeriVax-JE).

scientific article published on 27 July 2007

Crystal structure and activity of Kunjin virus NS3 helicase; protease and helicase domain assembly in the full length NS3 protein

scientific article

Crystal structure of the RNA polymerase domain of the West Nile virus non-structural protein 5

scientific article

DNA vaccine coding for the full-length infectious Kunjin virus RNA protects mice against the New York strain of West Nile virus

scientific article

De Novo Generation and Characterization of New Zika Virus Isolate Using Sequence Data from a Microcephaly Case

scientific article

Determinants of Zika virus host tropism uncovered by deep mutational scanning

scientific article published on 18 March 2019

Determinants of attenuation in the envelope protein of the flavivirus Alfuy

scientific article

Differential Diagnosis of Flavivirus Infections in Horses Using Viral Envelope Protein Domain III Antigens in Enzyme-Linked Immunosorbent Assay

scientific article published on 30 October 2017

Ebola virus glycoprotein GP is not cytotoxic when expressed constitutively at a moderate level.

scientific article published on May 2006

Effect of Wolbachia on replication of West Nile virus in a mosquito cell line and adult mosquitoes

scientific article

Encapsidation of the Flavivirus Kunjin Replicon RNA by Using a Complementation System Providing Kunjin Virus Structural Proteins in trans ⬇️

scientific article published on July 1, 1998

Evaluation of recombinant Kunjin replicon SIV vaccines for protective efficacy in macaques

scientific article published on 12 February 2008

Expression and purification of enzymatically active recombinant RNA-dependent RNA polymerase (NS5) of the flavivirus Kunjin

scientific article published in March 2001

Expression and purification of the seven nonstructural proteins of the flavivirus Kunjin in the E. coli and the baculovirus expression systems

scientific article published on September 1996

Expression of mosquito microRNA Aae-miR-2940-5p is downregulated in response to West Nile virus infection to restrict viral replication

scientific article published on 14 May 2014

Fetal Brain Infection Is Not a Unique Characteristic of Brazilian Zika Viruses

article

Full genome sequence of Rocio virus reveal substantial variations from the prototype Rocio virus SPH 34675 sequence

scientific article published on 22 September 2017

Functional non-coding RNAs derived from the flavivirus 3' untranslated region.

scientific article published on 07 February 2015

Generating flavivirus vaccine candidates by modulating interferon sensitivity

scientific article published on September 2009

Generation of CTL responses using Kunjin replicon RNA.

scientific article published in February 2003

Genetic characterization of Cacipacoré virus from ticks collected in São Paulo State, Brazil

scientific article published on 20 February 2017

Helicase Domain of West Nile Virus NS3 Protein Plays a Role in Inhibition of Type I Interferon Signalling.

scientific article published on 2 November 2017

Human MicroRNA miR-532-5p Exhibits Antiviral Activity against West Nile Virus via Suppression of Host Genes SESTD1 and TAB3 Required for Virus Replication

scientific article

Identification of residues in West Nile virus pre-membrane protein that influence viral particle secretion and virulence

scientific article

Immunization with recombinant vaccinia viruses expressing structural and part of the nonstructural region of tick-borne encephalitis virus cDNA protect mice against lethal encephalitis

scientific article published on January 1996

Increased expression of capsid protein in trans enhances production of single-round infectious particles by West Nile virus DNA vaccine candidate

scientific article published on 23 June 2014

Infectious DNAs derived from insect-specific flavivirus genomes enable identification of pre- and post-entry host restrictions in vertebrate cells

scientific article (publication date: 7 June 2017)

Inhibition of interferon signaling by the New York 99 strain and Kunjin subtype of West Nile virus involves blockage of STAT1 and STAT2 activation by nonstructural proteins

scientific article (publication date: February 2005)

Interferon response factors 3 and 7 protect against Chikungunya virus hemorrhagic fever and shock

scientific article

Internal ribosome entry site-based attenuation of a flavivirus candidate vaccine and evaluation of the effect of beta interferon coexpression on vaccine properties

scientific article published on 04 December 2013

Kunjin RNA replication and applications of Kunjin replicons

scientific article published on January 2003

Kunjin Virus Replicon Vectors for Human Immunodeficiency Virus Vaccine Development

scientific article (publication date: July 2003)

Kunjin replicon-based simian immunodeficiency virus gag vaccines

scientific article published on 21 April 2008

Kunjin virus replicon vaccine vectors induce protective CD8+ T-cell immunity

scientific article

Kunjin virus replicon-based vaccines expressing Ebola virus glycoprotein GP protect the guinea pig against lethal Ebola virus infection.

scientific article

Kunjin virus replicons: an RNA-based, non-cytopathic viral vector system for protein production, vaccine and gene therapy applications

scientific article

Last 20 aa of the West Nile virus NS1' protein are responsible for its retention in cells and the formation of unique heat-stable dimers

scientific article

Loop de loop: viral RNA evades IFIT1 targeting

scientific article published on 13 March 2014

Molecular and Functional Analyses of Kunjin Virus Infectious cDNA Clones Demonstrate the Essential Roles for NS2A in Virus Assembly and for a Nonconservative Residue in NS3 in RNA Replication

scientific article

Monoclonal antibodies to the West Nile virus NS5 protein map to linear and conformational epitopes in the methyltransferase and polymerase domains

scientific article published on 26 August 2009

Multiple immune factors are involved in controlling acute and chronic chikungunya virus infection

scientific article

Mutational analysis of Aedes aegypti Dicer 2 provides insights into the biogenesis of antiviral exogenous small interfering RNAs

scientific article published on 06 January 2022

NS1' colocalizes with NS1 and can substitute for NS1 in West Nile virus replication

scientific article published on 12 June 2013

NS1' of Flaviviruses in the Japanese Encephalitis Virus Serogroup Is a Product of Ribosomal Frameshifting and Plays a Role in Viral Neuroinvasiveness

scientific article

Nanopatch-targeted skin vaccination against West Nile Virus and Chikungunya virus in mice

scientific article published on August 2010

Nascent flavivirus RNA colocalized in situ with double-stranded RNA in stable replication complexes

scientific article published on May 1999

Neuroinvasiveness of the MR766 strain of Zika virus in IFNAR-/- mice maps to prM residues conserved amongst African genotype viruses

scientific article published on 26 July 2021

Noncoding flavivirus RNA displays RNA interference suppressor activity in insect and Mammalian cells

scientific article

Noncoding subgenomic flavivirus RNA: multiple functions in West Nile virus pathogenesis and modulation of host responses

scientific article published on 27 January 2014

Noncytopathic flavivirus replicon RNA-based system for expression and delivery of heterologous genes

scientific article published on March 1999

Post-translational regulation and modifications of flavivirus structural proteins

scientific article

Preliminary crystallographic characterization of an RNA helicase from Kunjin virus

scientific article

Prime-boost vaccinations using recombinant flavivirus replicon and vaccinia virus vaccines: an ELISPOT analysis

scientific article published on 3 August 2010

Programmed ribosomal frameshift alters expression of west nile virus genes and facilitates virus replication in birds and mosquitoes

scientific article

Proteins C and NS4B of the flavivirus Kunjin translocate independently into the nucleus

scientific article published on July 1997

RNA structures required for production of subgenomic flavivirus RNA

scientific article (publication date: November 2010)

Recombinant Kunjin virus replicon vaccines induce protective T-cell immunity against human papillomavirus 16 E7-expressing tumour

scientific article published on February 2004

Regulated Cleavages at the West Nile Virus NS4A-2K-NS4B Junctions Play a Major Role in Rearranging Cytoplasmic Membranes and Golgi Trafficking of the NS4A Protein

scientific article

Role of Nonstructural Protein NS2A in Flavivirus Assembly

scientific article

Semliki forest virus and Kunjin virus RNA replicons elicit comparable cellular immunity but distinct humoral immunity

scientific article

Significance in Replication of the Terminal Nucleotides of the Flavivirus Genome

scientific article

Single-round infectious particles enhance immunogenicity of a DNA vaccine against West Nile virus

scientific article published on 20 April 2008

Stable high-level expression of heterologous genes in vitro and in vivo by noncytopathic DNA-based Kunjin virus replicon vectors

scientific article (publication date: May 2000)

Structural analysis of 3’UTRs in insect flaviviruses reveals novel determinants of sfRNA biogenesis and provides new insights into flavivirus evolution

scientific article published in 2022

Subcellular localization and some biochemical properties of the flavivirus Kunjin nonstructural proteins NS2A and NS4A.

scientific article published on June 1998

Successful post-exposure prophylaxis of Ebola infected non-human primates using Ebola glycoprotein-specific equine IgG.

scientific article published on 03 February 2017

Systematic analysis of viral genes responsible for differential virulence between American and Australian West Nile virus strains

scientific article published on 27 January 2015

Tetracycline-inducible packaging cell line for production of flavivirus replicon particles

scientific article

The I22V and L72S substitutions in West Nile virus prM protein promote enhanced prM/E heterodimerisation and nucleocapsid incorporation

scientific article published on 7 May 2015

The NS5 Protein of the Virulent West Nile Virus NY99 Strain Is a Potent Antagonist of Type I Interferon-Mediated JAK-STAT Signaling

scientific article

The West Nile virus assembly process evades the conserved antiviral mechanism of the interferon-induced MxA protein

scientific article published on 22 October 2013

The distinguishing NS5-M114V mutation in American Zika virus isolates has negligible impacts on virus replication and transmission potential

scientific article published in 2022

The in vitro and in vivo antiviral properties of combined monoterpene alcohols against West Nile virus infection.

scientific article published on 3 May 2016

The naturally attenuated Kunjin strain of West Nile virus shows enhanced sensitivity to the host type I interferon response

scientific article published on 16 March 2011

Translation of the Flavivirus Kunjin NS3 Gene in cis but Not Its RNA Sequence or Secondary Structure Is Essential for Efficient RNA Packaging

scientific article (publication date: November 2006)

Ultrastructure of Kunjin virus-infected cells: colocalization of NS1 and NS3 with double-stranded RNA, and of NS2B with NS3, in virus-induced membrane structures ⬇️

scientific article published on September 1, 1997

Using a bioaerosol personal sampler in combination with real-time PCR analysis for rapid detection of airborne viruses

article

Viral RNA intermediates as targets for detection and discovery of novel and emerging mosquito-borne viruses.

scientific article

Viral determinants in the NS3 helicase and 2K peptide that promote West Nile virus resistance to antiviral action of 2',5'-oligoadenylate synthetase 1b.

scientific article published on 25 January 2010

Virulence and Evolution of West Nile Virus, Australia, 1960-2012

scientific article

Virulence determinants between New York 99 and Kunjin strains of West Nile virus

scientific article

West Nile virus NS2A protein facilitates virus-induced apoptosis independently of interferon response

scientific article published on 31 October 2012

West Nile virus core protein; tetramer structure and ribbon formation.

scientific article

West Nile virus encodes a microRNA-like small RNA in the 3' untranslated region which up-regulates GATA4 mRNA and facilitates virus replication in mosquito cells

scientific article published on 12 November 2011

West Nile virus genome with glycosylated envelope protein and deletion of alpha helices 1, 2, and 4 in the capsid protein is noninfectious and efficiently secretes subviral particles.

scientific article published on 18 September 2013

West Nile virus infection and interferon alpha treatment alter the spectrum and the levels of coding and noncoding host RNAs secreted in extracellular vesicles.

scientific article published on 10 June 2019

West Nile virus noncoding subgenomic RNA contributes to viral evasion of the type I interferon-mediated antiviral response

scientific article

West Nile virus vaccines

scientific article

West Nile virus-induced cytoplasmic membrane structures provide partial protection against the interferon-induced antiviral MxA protein

scientific article published on November 2007

Wolbachia infection modifies the profile, shuttling and structure of microRNAs in a mosquito cell line

scientific article

Zika virus noncoding RNA cooperates with the viral protein NS5 to inhibit STAT1 phosphorylation and facilitate viral pathogenesis

scientific article published in 2022

cis- and trans-acting elements in flavivirus RNA replication

scientific article (publication date: April 2000)

pDNA-lipoplexes engrafted with flagellin-related peptide induce potent immunity and anti-tumour effects

scientific article

trans -Complementation of Flavivirus RNA Polymerase Gene NS5 by Using Kunjin Virus Replicon-Expressing BHK Cells ⬇️

scientific article published on September 1, 1998

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