List of works - Per-Ake Nygren

1H, 13C and 15N resonance assignments of an affibody-target complex

scientific article published on 01 November 2002

A dual expression system for the generation, analysis and purification of antibodies to a repeated sequence of the Plasmodium falciparum antigen Pf155/RESA.

scientific article published in November 1989

A general strategy for polymerization, assembly and expression of epitope-carrying peptides applied to the Plasmodium falciparum antigen Pf155/RESA.

scientific article published in May 1990

A novel affinity gene fusion system allowing protein A-based recovery of non-immunoglobulin gene products

scientific article

Affibody Molecules in Biotechnological and Medical Applications

scientific article

Affibody molecules in protein capture microarrays: evaluation of multidomain ligands and different detection formats.

scientific article

Affibody protein capture microarrays: synthesis and evaluation of random and directed immobilization of affibody molecules

scientific article published in June 2005

Affibody-beta-galactosidase immunoconjugates produced as soluble fusion proteins in the Escherichia coli cytosol

scientific article published in October 2003

Affibody-mediated retention of the epidermal growth factor receptor in the secretory compartments leads to inhibition of phosphorylation in the kinase domain.

scientific article

Affinity ligands from biological combinatorial libraries.

scientific article

Affinity maturation of a TNFalpha-binding affibody molecule by Darwinian survival selection.

scientific article

Affinity recovery of eight HER2-binding affibody variants using an anti-idiotypic affibody molecule as capture ligand.

scientific article

Affinity-based entrapment of the HER2 receptor in the endoplasmic reticulum using an affibody molecule.

scientific article

All individual domains of staphylococcal protein A show Fab binding

scientific article published in January 1998

An affibody in complex with a target protein: Structure and coupled folding

scientific article (publication date: 18 March 2003)

Analysis and use of the serum albumin binding domains of streptococcal protein G

scientific article published on 01 April 1988

Anti-idiotypic protein domains selected from protein A-based affibody libraries

scientific article

Binding proteins from alternative scaffolds

scientific article published in July 2004

Biophysical characterization of Z(SPA-1)--a phage-display selected binder to protein A.

scientific article

Charge engineering of a protein domain to allow efficient ion-exchange recovery

scientific article published on 01 October 2000

Chemical synthesis of triple-labelled three-helix bundle binding proteins for specific fluorescent detection of unlabelled protein.

scientific article

Chromophore pre-maturation for improved speed and sensitivity of split-GFP monitoring of protein secretion

scientific article published in Scientific Reports

Combinatorial expression vector engineering for tuning of recombinant protein production in Escherichia coli

scientific article published on 30 January 2007

Competitive elution of protein A fusion proteins allows specific recovery under mild conditions.

scientific article

Consequences of membrane protein overexpression in Escherichia coli.

scientific article published on 19 April 2007

Construction and characterization of affibody-Fc chimeras produced in Escherichia coli.

scientific article

Cyclin A-cdc2 kinase does not trigger but delays cyclin degradation in interphase extracts of amphibian eggs.

scientific article published on May 1992

Decreased immune reactivity towards a knobless, affibody-targeted adenovirus type 5 vector

scientific article published on 12 October 2006

Design and production of recombinant subunit vaccines.

scientific article

Detection and isolation of recombinant proteins based on binding affinity of reporter: protein A

scientific article published on 01 January 1997

Detection of antigens using a protein-DNA chimera developed by enzymatic covalent bonding with phiX gene A*

scientific article published on 16 May 2012

Detection of mutations in PCR products from clinical samples by surface plasmon resonance

scientific article published in January 1997

Differential degradation of a recombinant albumin-binding receptor in Escherichia coli.

scientific article published in July 1991

Direct and competitive kinetic analysis of the interaction between human IgG1 and a one domain analogue of protein A

scientific article published on 01 June 1995

Display of active subtilisin 309 on phage: analysis of parameters influencing the selection of subtilisin variants with changed substrate specificity from libraries using phosphonylating inhibitors.

scientific article

Engineering of a femtomolar affinity binding protein to human serum albumin

scientific article

Evaluation of different linker regions for multimerization and coupling chemistry for immobilization of a proteinaceous affinity ligand

scientific article published in December 2003

Extended in vivo half-life of human soluble complement receptor type 1 fused to a serum albumin-binding receptor

scientific article published on 01 April 1996

Fluorescence resonance energy transfer-based detection of analytes using antiidiotypic affinity protein pairs

scientific article published in November 2004

Fluorescence-microscopy-based image analysis for analyte-dependent particle doublet detection in a single-step immunoagglutination assay

scientific article published in March 2005

Fluorescent detection of beta-lactamase activity in living Escherichia coli cells via esterase supplementation

scientific article published in January 2005

Functional selection of phage displayed peptides for facilitated design of fusion tags improving aqueous two-phase partitioning of recombinant proteins

scientific article

Gene fragment polymerization gives increased yields of recombinant human proinsulin C-peptide

scientific article published on 01 April 1998

Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli.

scientific article

Genetic engineering of protein-peptide fusions for control of protein partitioning in thermoseparating aqueous two-phase systems

scientific article published on 01 January 1999

Heat-mediated activation of affinity-immobilized Taq DNA polymerase.

scientific article published on April 1997

High antibody responses in rabbits immunized with influenza virus ISCOMs containing a repeated sequence of the Plasmodium falciparum antigen Pf155/RESA.

scientific article published in June 1991

Human immunoglobulin A (IgA)-specific ligands from combinatorial engineering of protein A.

scientific article

Hydrophobicity engineering to facilitate surface display of heterologous gene products on Staphylococcus xylosus

scientific article published on 01 October 1995

In vitro selection of enzymatically active lipase variants from phage libraries using a mechanism-based inhibitor.

scientific article

Inhibition of the CD28-CD80 co-stimulation signal by a CD28-binding affibody ligand developed by combinatorial protein engineering.

scientific article published in September 2003

Inhibitory effects of H-Ras/Raf-1-binding affibody molecules on synovial cell function.

scientific article published on 11 November 2014

Integrated bioprocess for production of human proinsulin C-peptide via heat release of an intracellular heptameric fusion protein

scientific article published on 01 January 2000

Integrated strategy for selective expanded bed ion-exchange adsorption and site-specific protein processing using gene fusion technology.

scientific article

Kinetic characterization of the interaction of the Z-fragment of protein A with mouse-IgG3 in a volume in chemical space

scientific article published in November 1999

Lysis of Staphylococcal Cells by Modular Lysin Domains Linked via a Non-covalent Barnase-Barstar Interaction Bridge

scientific article published on 22 March 2019

Microbead display of proteins by cell-free expression of anchored DNA.

scientific article published in December 2003

Minimum information about a protein affinity reagent (MIAPAR)

scientific article published on 01 July 2010

Monitored whole gene in vitro evolution of an anti-hRaf-1 affibody molecule towards increased binding affinity.

scientific article

Multiple affinity domains for the detection, purification and immobilization of recombinant proteins.

scientific article

Mutational scanning of PCR products by subtractive oligonucleotide hybridization analysis

scientific article published in February 1999

Partitioning of peptides and recombinant protein-peptide fusions in thermoseparating aqueous two-phase systems: effect of peptide primary structure

scientific article published on 01 June 2000

Pneumolysin binds to the mannose receptor C type 1 (MRC-1) leading to anti-inflammatory responses and enhanced pneumococcal survival

scientific article published on 12 November 2018

Predominance of H-2d- and H-2k-restricted T-cell epitopes in the highly repetitive Plasmodium falciparum antigen Pf332 ⬇️

scientific article published on April 1, 1997

Production of a thermostable DNA polymerase by site-specific cleavage of a heat-eluted affinity fusion protein

scientific article published on 01 February 1997

Proline substitution independently enhances H-2D(b) complex stabilization and TCR recognition of melanoma-associated peptides

scientific article

ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome

article

Real-Time Monitoring of DNA Manipulations Using Biosensor Technology

scientific article published on 01 January 1995

Recombinant spider silk genetically functionalized with affinity domains

scientific article published on 8 April 2014

Ribosome display selection of a murine IgG₁ Fab binding affibody molecule allowing species selective recovery of monoclonal antibodies.

scientific article

Scaffolds for engineering novel binding sites in proteins ⬇️

scientific article published on August 1, 1997

Selection and characterisation of affibody molecules inhibiting the interaction between Ras and Raf in vitro.

scientific article

Selection of TNF-alpha binding affibody molecules using a beta-lactamase protein fragment complementation assay.

scientific article

Selectivity analysis of single binder assays used in plasma protein profiling

scientific article published on 18 November 2013

Site-Specific Photoconjugation of Beta-Lactamase Fragments to Monoclonal Antibodies Enables Sensitive Analyte Detection via Split-Enzyme Complementation

scientific article published on 14 March 2018

Site-specific and reversible anchoring of active proteins onto cellulose using a cellulosome-like complex

scientific article published in April 2004

Stabilization of recombinant proteins from proteolytic degradation in Escherichia coli using a dual affinity fusion strategy.

scientific article published in December 1991

Strategies for gene fusions

scientific article published on 01 January 1997

Structural and functional analysis of the human IgG-Fab receptor activity of streptococcal protein G

scientific article published on 01 October 1991

Structural basis for recognition by an in vitro evolved affibody

scientific article

Structure, specificity, and mode of interaction for bacterial albumin-binding modules

scientific article

Successive crystal structure snapshots suggest the basis for MHC class I peptide loading and editing by tapasin

scientific article published on 26 February 2019

Surface display of a functional single-chain Fv antibody on staphylococci.

scientific article published on March 1996

Systemic administration of monovalent follistatin-like 3-Fc-fusion protein increases muscle mass in mice

scientific article published on 14 May 2021

Tailor-making a protein a-derived domain for efficient site-specific photocoupling to Fc of mouse IgG₁.

scientific article

The BR domain of PsrP interacts with extracellular DNA to promote bacterial aggregation; structural insights into pneumococcal biofilm formation

scientific article

The serum albumin-binding domain of streptococcal protein G is a three-helical bundle: a heteronuclear NMR study

scientific article published on 01 January 1996

The serum albumin-binding region of streptococcal protein G: a bacterial fusion partner with carrier-related properties

scientific article published in February 1997

Tumor cell targeted gene delivery by adenovirus 5 vectors carrying knobless fibers with antibody-binding domains.

scientific article

Tuning antiviral CD8 T-cell response via proline-altered peptide ligand vaccination

scientific article published on 04 May 2020

Unexpected T-cell recognition of an altered peptide ligand is driven by reversed thermodynamics

scientific article published on 20 September 2012

List of works by Per-Ake Nygren

1H, 13C and 15N resonance assignments of an affibody-target complex

A dual expression system for the generation, analysis and purification of antibodies to a repeated sequence of the Plasmodium falciparum antigen Pf155/RESA.

A general strategy for polymerization, assembly and expression of epitope-carrying peptides applied to the Plasmodium falciparum antigen Pf155/RESA.

A novel affinity gene fusion system allowing protein A-based recovery of non-immunoglobulin gene products

Affibody Molecules in Biotechnological and Medical Applications

Affibody molecules in protein capture microarrays: evaluation of multidomain ligands and different detection formats.

Affibody protein capture microarrays: synthesis and evaluation of random and directed immobilization of affibody molecules

Affibody-beta-galactosidase immunoconjugates produced as soluble fusion proteins in the Escherichia coli cytosol

Affibody-mediated retention of the epidermal growth factor receptor in the secretory compartments leads to inhibition of phosphorylation in the kinase domain.

Affinity ligands from biological combinatorial libraries.

Affinity maturation of a TNFalpha-binding affibody molecule by Darwinian survival selection.

Affinity recovery of eight HER2-binding affibody variants using an anti-idiotypic affibody molecule as capture ligand.

Affinity-based entrapment of the HER2 receptor in the endoplasmic reticulum using an affibody molecule.

All individual domains of staphylococcal protein A show Fab binding

An affibody in complex with a target protein: Structure and coupled folding

Analysis and use of the serum albumin binding domains of streptococcal protein G

Anti-idiotypic protein domains selected from protein A-based affibody libraries

Binding proteins from alternative scaffolds

Biophysical characterization of Z(SPA-1)--a phage-display selected binder to protein A.

Charge engineering of a protein domain to allow efficient ion-exchange recovery

Chemical synthesis of triple-labelled three-helix bundle binding proteins for specific fluorescent detection of unlabelled protein.

Chromophore pre-maturation for improved speed and sensitivity of split-GFP monitoring of protein secretion

Combinatorial expression vector engineering for tuning of recombinant protein production in Escherichia coli

Competitive elution of protein A fusion proteins allows specific recovery under mild conditions.

Consequences of membrane protein overexpression in Escherichia coli.

Construction and characterization of affibody-Fc chimeras produced in Escherichia coli.

Cyclin A-cdc2 kinase does not trigger but delays cyclin degradation in interphase extracts of amphibian eggs.

Decreased immune reactivity towards a knobless, affibody-targeted adenovirus type 5 vector

Design and production of recombinant subunit vaccines.

Detection and isolation of recombinant proteins based on binding affinity of reporter: protein A

Detection of antigens using a protein-DNA chimera developed by enzymatic covalent bonding with phiX gene A*

Detection of mutations in PCR products from clinical samples by surface plasmon resonance

Differential degradation of a recombinant albumin-binding receptor in Escherichia coli.

Direct and competitive kinetic analysis of the interaction between human IgG1 and a one domain analogue of protein A

Display of active subtilisin 309 on phage: analysis of parameters influencing the selection of subtilisin variants with changed substrate specificity from libraries using phosphonylating inhibitors.

Engineering of a femtomolar affinity binding protein to human serum albumin

Evaluation of different linker regions for multimerization and coupling chemistry for immobilization of a proteinaceous affinity ligand

Extended in vivo half-life of human soluble complement receptor type 1 fused to a serum albumin-binding receptor

Fluorescence resonance energy transfer-based detection of analytes using antiidiotypic affinity protein pairs

Fluorescence-microscopy-based image analysis for analyte-dependent particle doublet detection in a single-step immunoagglutination assay

Fluorescent detection of beta-lactamase activity in living Escherichia coli cells via esterase supplementation

Functional selection of phage displayed peptides for facilitated design of fusion tags improving aqueous two-phase partitioning of recombinant proteins

Gene fragment polymerization gives increased yields of recombinant human proinsulin C-peptide

Genetic design for facilitated production and recovery of recombinant proteins in Escherichia coli.

Genetic engineering of protein-peptide fusions for control of protein partitioning in thermoseparating aqueous two-phase systems

Heat-mediated activation of affinity-immobilized Taq DNA polymerase.

High antibody responses in rabbits immunized with influenza virus ISCOMs containing a repeated sequence of the Plasmodium falciparum antigen Pf155/RESA.

Human immunoglobulin A (IgA)-specific ligands from combinatorial engineering of protein A.

Hydrophobicity engineering to facilitate surface display of heterologous gene products on Staphylococcus xylosus

In vitro selection of enzymatically active lipase variants from phage libraries using a mechanism-based inhibitor.

Inhibition of the CD28-CD80 co-stimulation signal by a CD28-binding affibody ligand developed by combinatorial protein engineering.

Inhibitory effects of H-Ras/Raf-1-binding affibody molecules on synovial cell function.

Integrated bioprocess for production of human proinsulin C-peptide via heat release of an intracellular heptameric fusion protein

Integrated strategy for selective expanded bed ion-exchange adsorption and site-specific protein processing using gene fusion technology.

Kinetic characterization of the interaction of the Z-fragment of protein A with mouse-IgG3 in a volume in chemical space

Lysis of Staphylococcal Cells by Modular Lysin Domains Linked via a Non-covalent Barnase-Barstar Interaction Bridge

Microbead display of proteins by cell-free expression of anchored DNA.

Minimum information about a protein affinity reagent (MIAPAR)

Monitored whole gene in vitro evolution of an anti-hRaf-1 affibody molecule towards increased binding affinity.

Multiple affinity domains for the detection, purification and immobilization of recombinant proteins.

Mutational scanning of PCR products by subtractive oligonucleotide hybridization analysis

Partitioning of peptides and recombinant protein-peptide fusions in thermoseparating aqueous two-phase systems: effect of peptide primary structure

Pneumolysin binds to the mannose receptor C type 1 (MRC-1) leading to anti-inflammatory responses and enhanced pneumococcal survival

Predominance of H-2d- and H-2k-restricted T-cell epitopes in the highly repetitive Plasmodium falciparum antigen Pf332 ⬇️

Production of a thermostable DNA polymerase by site-specific cleavage of a heat-eluted affinity fusion protein

Proline substitution independently enhances H-2D(b) complex stabilization and TCR recognition of melanoma-associated peptides

ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome

Real-Time Monitoring of DNA Manipulations Using Biosensor Technology

Recombinant spider silk genetically functionalized with affinity domains

Ribosome display selection of a murine IgG₁ Fab binding affibody molecule allowing species selective recovery of monoclonal antibodies.

Scaffolds for engineering novel binding sites in proteins ⬇️

Selection and characterisation of affibody molecules inhibiting the interaction between Ras and Raf in vitro.

Selection of TNF-alpha binding affibody molecules using a beta-lactamase protein fragment complementation assay.

Selectivity analysis of single binder assays used in plasma protein profiling

Site-Specific Photoconjugation of Beta-Lactamase Fragments to Monoclonal Antibodies Enables Sensitive Analyte Detection via Split-Enzyme Complementation

Site-specific and reversible anchoring of active proteins onto cellulose using a cellulosome-like complex

Stabilization of recombinant proteins from proteolytic degradation in Escherichia coli using a dual affinity fusion strategy.

Strategies for gene fusions

Structural and functional analysis of the human IgG-Fab receptor activity of streptococcal protein G