List of works - John Löfblom

188Re-ZHER2:V2, a promising affibody-based targeting agent against HER2-expressing tumors: preclinical assessment.

scientific article published on 2 October 2014

A new prodrug form of Affibody molecules (pro-Affibody) is selectively activated by cancer-associated proteases

scientific article published on 7 October 2014

A novel affinity protein selection system based on staphylococcal cell surface display and flow cytometry.

scientific article

A protease substrate profiling method that links site-specific proteolysis with antibiotic resistance.

scientific article

Affibody Molecules in Biotechnological and Medical Applications

scientific article

Affibody molecules: engineered proteins for therapeutic, diagnostic and biotechnological applications.

scientific article published on 11 April 2010

Affibody-mediated PET imaging of HER3 expression in malignant tumours.

scientific article

Affibody‑mediated imaging of EGFR expression in prostate cancer using radiocobalt‑labeled DOTA‑ZEGFR:2377

scientific article published on 15 October 2018

An Affibody Molecule Is Actively Transported into the Cerebrospinal Fluid via Binding to the Transferrin Receptor

scientific article published on 23 April 2020

An engineered autotransporter-based surface expression vector enables efficient display of Affibody molecules on OmpT-negative E. coli as well as protease-mediated secretion in OmpT-positive strains

scientific article published on 30 December 2014

Autotransporter-Mediated Display of a Naïve Affibody Library on the Outer Membrane of Escherichia coli

scientific article published on 25 September 2018

Bacterial display in combinatorial protein engineering.

scientific article

Benefit of Later-Time-Point PET Imaging of HER3 Expression Using Optimized Radiocobalt-Labeled Affibody Molecules

scientific article published on 13 March 2020

Cellular effects of HER3-specific affibody molecules

scientific article (publication date: 2012)

Combining phage and staphylococcal surface display for generation of ErbB3-specific Affibody molecules.

scientific article

Comparative Evaluation of Affibody Molecules for Radionuclide Imaging of in Vivo Expression of Carbonic Anhydrase IX.

scientific article published on 16 August 2016

Comparative Evaluation of Two DARPin Variants: Effect of Affinity, Size, and Label on Tumor Targeting Properties

scientific article published on 08 February 2019

Comparative evaluation of 111In-labeled NOTA‑conjugated affibody molecules for visualization of HER3 expression in malignant tumors.

scientific article published on 9 June 2015

Comparative evaluation of affibody- and antibody fragments-based CAIX imaging probes in mice bearing renal cell carcinoma xenografts

scientific article published on 17 October 2019

Comparison of tumor‑targeting properties of directly and indirectly radioiodinated designed ankyrin repeat protein (DARPin) G3 variants for molecular imaging of HER2

scientific article published on 11 February 2019

Cyclic versus Noncyclic Chelating Scaffold for 89Zr-Labeled ZEGFR:2377 Affibody Bioconjugates Targeting Epidermal Growth Factor Receptor Overexpression

scientific article published on 21 November 2017

Development and characterization of small bispecific albumin-binding domains with high affinity for ErbB3.

scientific article

Directed evolution of the 3C protease from coxsackievirus using a novel fluorescence-assisted intracellular method

scientific article published on 01 February 2019

Engineering bispecificity into a single albumin-binding domain

scientific article

Engineering of a bispecific affibody molecule towards HER2 and HER3 by addition of an albumin-binding domain allows for affinity purification and in vivo half-life extension.

scientific article published on 23 April 2014

Engineering of bispecific affinity proteins with high affinity for ERBB2 and adaptable binding to albumin

scientific article

Epitope mapping of antibodies using bacterial surface display.

scientific article published on 23 November 2008

Epitope mapping using gram-positive surface display.

scientific article

Evaluating the Therapeutic Efficacy of Mono- and Bivalent Affibody-Based Fusion Proteins Targeting HER3 in a Pancreatic Cancer Xenograft Model

scientific article published on 13 June 2020

Evaluation of a radiocobalt-labelled affibody molecule for imaging of human epidermal growth factor receptor 3 expression

scientific article published on 11 October 2017

Evaluation of the Therapeutic Potential of a HER3-Binding Affibody Construct TAM-HER3 in Comparison with a Monoclonal Antibody, Seribantumab

scientific article published on 25 July 2018

Exploring epitopes of antibodies toward the human tryptophanyl-tRNA synthetase

scientific article published on 11 November 2009

Feasibility of imaging of epidermal growth factor receptor expression with ZEGFR:2377 affibody molecule labeled with 99mTc using a peptide-based cysteine-containing chelator.

scientific article published on 05 October 2016

Fine affinity discrimination by normalized fluorescence activated cell sorting in staphylococcal surface display

scientific article published in July 2005

Flow-cytometric screening of aggregation-inhibitors using a fluorescence-assisted intracellular method.

scientific article

Identification of proteins that specifically recognize and bind protofibrillar aggregates of amyloid-β.

scientific article

Imaging of HER3-expressing xenografts in mice using a (99m)Tc(CO) 3-HEHEHE-Z HER3:08699 affibody molecule.

scientific article published on 13 March 2014

Improved contrast of affibody-mediated imaging of HER3 expression in mouse xenograft model through co-injection of a trivalent affibody for in vivo blocking of hepatic uptake

scientific article published on 01 May 2019

In vivo evaluation of a novel format of a bivalent HER3-targeting and albumin-binding therapeutic affibody construct.

scientific article published on 23 February 2017

Increase in negative charge of 68Ga/chelator complex reduces unspecific hepatic uptake but does not improve imaging properties of HER3-targeting affibody molecules

scientific article published on 27 November 2019

Influence of Molecular Design on the Targeting Properties of ABD-Fused Mono- and Bi-Valent Anti-HER3 Affibody Therapeutic Constructs

scientific article published on 11 October 2018

Influence of Residualizing Properties of the Radiolabel on Radionuclide Molecular Imaging of HER3 Using Affibody Molecules

scientific article published on 15 February 2020

Influence of composition of cysteine-containing peptide-based chelators on biodistribution of 99mTc-labeled anti-EGFR affibody molecules.

scientific article

Inhibiting HER3-mediated tumor cell growth with affibody molecules engineered to low picomolar affinity by position-directed error-prone PCR-like diversification

scientific article

Insights from engineering the Affibody-Fc interaction with a computational-experimental method

scientific article

Investigating affinity-maturation strategies and reproducibility of fluorescence-activated cell sorting using a recombinant ADAPT library displayed on staphylococci.

scientific article

Molecular Design of HER3-Targeting Affibody Molecules: Influence of Chelator and Presence of HEHEHE-Tag on Biodistribution of Ga-Labeled Tracers ⬇️

scientific article published on 02 March 2019

Non-immunoglobulin based protein scaffolds.

scientific article published on 02 July 2011

Novel affinity binders for neutralization of vascular endothelial growth factor (VEGF) signaling

scientific article published on 9 November 2015

Optimal composition and position of histidine-containing tags improves biodistribution of 99mTc-labeled DARPin G3

scientific article published on 28 June 2019

Optimization of HER3 expression imaging using affibody molecules: Influence of chelator for labeling with indium-111

scientific article published in Scientific Reports

Optimization of electroporation-mediated transformation: Staphylococcus carnosus as model organism.

scientific article

PET imaging of epidermal growth factor receptor expression in tumours using 89Zr-labelled ZEGFR:2377 affibody molecules.

scientific article published on 2 February 2016

Protease substrate profiling using bacterial display of self-blocking affinity proteins and flow-cytometric sorting

scientific article

Radionuclide imaging of VEGFR2 in glioma vasculature using biparatopic affibody conjugate: proof-of-principle in a murine model

scientific article published on 07 August 2018

Robust expression of the human neonatal Fc receptor in a truncated soluble form and as a full-length membrane-bound protein in fusion with eGFP

scientific article

Selection of an optimal cysteine-containing peptide-based chelator for labeling of affibody molecules with (188)Re.

scientific article published on 26 September 2014

Simplified characterization through site-specific protease-mediated release of affinity proteins selected by staphylococcal display.

scientific article

Simultaneous targeting of two ligand-binding sites on VEGFR2 using biparatopic Affibody molecules results in dramatically improved affinity

scientific article published on 17 December 2014

Staphylococcal display for combinatorial protein engineering of a head-to-tail affibody dimer binding the Alzheimer amyloid-β peptide.

scientific article

Staphylococcal surface display in combinatorial protein engineering and epitope mapping of antibodies.

scientific article

Staphylococcus carnosus: from starter culture to protein engineering platform.

scientific article

Surface display of a single-domain antibody library on Gram-positive bacteria.

scientific article

Targeting HER3 using mono- and bispecific antibodies or alternative scaffolds.

scientific article

The use of radiocobalt as a label improves imaging of EGFR using DOTA-conjugated Affibody molecule.

scientific article

VEGFR2-Specific Ligands Based on Affibody Molecules Demonstrate Agonistic Effects when Tetrameric in the Soluble Form or Immobilized via Spider Silk

scientific article published on 13 November 2019

List of works by John Löfblom

188Re-ZHER2:V2, a promising affibody-based targeting agent against HER2-expressing tumors: preclinical assessment.

A new prodrug form of Affibody molecules (pro-Affibody) is selectively activated by cancer-associated proteases

A novel affinity protein selection system based on staphylococcal cell surface display and flow cytometry.

A protease substrate profiling method that links site-specific proteolysis with antibiotic resistance.

Affibody Molecules in Biotechnological and Medical Applications

Affibody molecules: engineered proteins for therapeutic, diagnostic and biotechnological applications.

Affibody-mediated PET imaging of HER3 expression in malignant tumours.

Affibody‑mediated imaging of EGFR expression in prostate cancer using radiocobalt‑labeled DOTA‑ZEGFR:2377

An Affibody Molecule Is Actively Transported into the Cerebrospinal Fluid via Binding to the Transferrin Receptor

An engineered autotransporter-based surface expression vector enables efficient display of Affibody molecules on OmpT-negative E. coli as well as protease-mediated secretion in OmpT-positive strains

Autotransporter-Mediated Display of a Naïve Affibody Library on the Outer Membrane of Escherichia coli

Bacterial display in combinatorial protein engineering.

Benefit of Later-Time-Point PET Imaging of HER3 Expression Using Optimized Radiocobalt-Labeled Affibody Molecules

Cellular effects of HER3-specific affibody molecules

Combining phage and staphylococcal surface display for generation of ErbB3-specific Affibody molecules.

Comparative Evaluation of Affibody Molecules for Radionuclide Imaging of in Vivo Expression of Carbonic Anhydrase IX.

Comparative Evaluation of Two DARPin Variants: Effect of Affinity, Size, and Label on Tumor Targeting Properties

Comparative evaluation of 111In-labeled NOTA‑conjugated affibody molecules for visualization of HER3 expression in malignant tumors.

Comparative evaluation of affibody- and antibody fragments-based CAIX imaging probes in mice bearing renal cell carcinoma xenografts

Comparison of tumor‑targeting properties of directly and indirectly radioiodinated designed ankyrin repeat protein (DARPin) G3 variants for molecular imaging of HER2

Cyclic versus Noncyclic Chelating Scaffold for 89Zr-Labeled ZEGFR:2377 Affibody Bioconjugates Targeting Epidermal Growth Factor Receptor Overexpression

Development and characterization of small bispecific albumin-binding domains with high affinity for ErbB3.

Directed evolution of the 3C protease from coxsackievirus using a novel fluorescence-assisted intracellular method

Engineering bispecificity into a single albumin-binding domain

Engineering of a bispecific affibody molecule towards HER2 and HER3 by addition of an albumin-binding domain allows for affinity purification and in vivo half-life extension.

Engineering of bispecific affinity proteins with high affinity for ERBB2 and adaptable binding to albumin

Epitope mapping of antibodies using bacterial surface display.

Epitope mapping using gram-positive surface display.

Evaluating the Therapeutic Efficacy of Mono- and Bivalent Affibody-Based Fusion Proteins Targeting HER3 in a Pancreatic Cancer Xenograft Model

Evaluation of a radiocobalt-labelled affibody molecule for imaging of human epidermal growth factor receptor 3 expression

Evaluation of the Therapeutic Potential of a HER3-Binding Affibody Construct TAM-HER3 in Comparison with a Monoclonal Antibody, Seribantumab

Exploring epitopes of antibodies toward the human tryptophanyl-tRNA synthetase

Feasibility of imaging of epidermal growth factor receptor expression with ZEGFR:2377 affibody molecule labeled with 99mTc using a peptide-based cysteine-containing chelator.

Fine affinity discrimination by normalized fluorescence activated cell sorting in staphylococcal surface display

Flow-cytometric screening of aggregation-inhibitors using a fluorescence-assisted intracellular method.

Identification of proteins that specifically recognize and bind protofibrillar aggregates of amyloid-β.

Imaging of HER3-expressing xenografts in mice using a (99m)Tc(CO) 3-HEHEHE-Z HER3:08699 affibody molecule.

Improved contrast of affibody-mediated imaging of HER3 expression in mouse xenograft model through co-injection of a trivalent affibody for in vivo blocking of hepatic uptake

In vivo evaluation of a novel format of a bivalent HER3-targeting and albumin-binding therapeutic affibody construct.

Increase in negative charge of 68Ga/chelator complex reduces unspecific hepatic uptake but does not improve imaging properties of HER3-targeting affibody molecules

Influence of Molecular Design on the Targeting Properties of ABD-Fused Mono- and Bi-Valent Anti-HER3 Affibody Therapeutic Constructs

Influence of Residualizing Properties of the Radiolabel on Radionuclide Molecular Imaging of HER3 Using Affibody Molecules

Influence of composition of cysteine-containing peptide-based chelators on biodistribution of 99mTc-labeled anti-EGFR affibody molecules.

Inhibiting HER3-mediated tumor cell growth with affibody molecules engineered to low picomolar affinity by position-directed error-prone PCR-like diversification

Insights from engineering the Affibody-Fc interaction with a computational-experimental method

Investigating affinity-maturation strategies and reproducibility of fluorescence-activated cell sorting using a recombinant ADAPT library displayed on staphylococci.

Molecular Design of HER3-Targeting Affibody Molecules: Influence of Chelator and Presence of HEHEHE-Tag on Biodistribution of Ga-Labeled Tracers ⬇️

Non-immunoglobulin based protein scaffolds.

Novel affinity binders for neutralization of vascular endothelial growth factor (VEGF) signaling

Optimal composition and position of histidine-containing tags improves biodistribution of 99mTc-labeled DARPin G3

Optimization of HER3 expression imaging using affibody molecules: Influence of chelator for labeling with indium-111

Optimization of electroporation-mediated transformation: Staphylococcus carnosus as model organism.

PET imaging of epidermal growth factor receptor expression in tumours using 89Zr-labelled ZEGFR:2377 affibody molecules.

Protease substrate profiling using bacterial display of self-blocking affinity proteins and flow-cytometric sorting

Radionuclide imaging of VEGFR2 in glioma vasculature using biparatopic affibody conjugate: proof-of-principle in a murine model

Robust expression of the human neonatal Fc receptor in a truncated soluble form and as a full-length membrane-bound protein in fusion with eGFP

Selection of an optimal cysteine-containing peptide-based chelator for labeling of affibody molecules with (188)Re.

Simplified characterization through site-specific protease-mediated release of affinity proteins selected by staphylococcal display.

Simultaneous targeting of two ligand-binding sites on VEGFR2 using biparatopic Affibody molecules results in dramatically improved affinity

Staphylococcal display for combinatorial protein engineering of a head-to-tail affibody dimer binding the Alzheimer amyloid-β peptide.

Staphylococcal surface display in combinatorial protein engineering and epitope mapping of antibodies.

Staphylococcus carnosus: from starter culture to protein engineering platform.

Surface display of a single-domain antibody library on Gram-positive bacteria.

Targeting HER3 using mono- and bispecific antibodies or alternative scaffolds.

The use of radiocobalt as a label improves imaging of EGFR using DOTA-conjugated Affibody molecule.

VEGFR2-Specific Ligands Based on Affibody Molecules Demonstrate Agonistic Effects when Tetrameric in the Soluble Form or Immobilized via Spider Silk