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List of works by Holger Puchta

A SRS2 homolog from Arabidopsis thaliana disrupts recombinogenic DNA intermediates and facilitates single strand annealing

scientific article published on November 2009

A homolog of ScRAD5 is involved in DNA repair and homologous recombination in Arabidopsis

scientific article

A homologue of the breast cancer-associated gene BARD1 is involved in DNA repair in plants

scientific article

A homologue of the breast cancer-associated gene BARD1 is involved in DNA repair in plants.

scientific article published in April 2007

A new strain of potato spindle tuber viroid (PSTVd-N) exhibits major sequence differences as compared to all other PSTVd strains sequenced so far.

scientific article

A transient assay in plant cells reveals a positive correlation between extrachromosomal recombination rates and length of homologous overlap

scientific article

Agrobacterium tumefaciens transfers single-stranded transferred DNA (T-DNA) into the plant cell nucleus

scientific article published on August 1994

An Arabidopsis FANCJ helicase homologue is required for DNA crosslink repair and rDNA repeat stability

scientific article published on 23 May 2019

An archaebacterial topoisomerase homolog not present in other eukaryotes is indispensable for cell proliferation of plants

scientific article published on 01 October 2002

An improved procedure for the rapid one-step-cloning of full-length viroid cDNA.

scientific article

Analysis of unknown DNA sequences by polymerase chain reaction (PCR) using a single specific primer and a standardized adaptor

scientific article published on 01 April 1991

Application of CRISPR/Cas to Understand Cis- and Trans-Regulatory Elements in Plants

article

Applying CRISPR/Cas for genome engineering in plants: the best is yet to come

scientific article published on 30 November 2016

AtRAD5A is a DNA translocase harboring a HIRAN domain which confers binding to branched DNA structures and is required for DNA repair in vivo

scientific article published on 26 July 2016

AtRECQ2, a RecQ helicase homologue from Arabidopsis thaliana, is able to disrupt various recombinogenic DNA structures in vitro

scientific article published in August 2008

BRCA2 is a mediator of RAD51- and DMC1-facilitated homologous recombination in Arabidopsis thaliana.

scientific article

BRCC36A is epistatic to BRCA1 in DNA crosslink repair and homologous recombination in Arabidopsis thaliana

scientific article

Biochemical characterization of AtRECQ3 reveals significant differences relative to other RecQ helicases

scientific article published on 15 September 2009

Biochemical characterization of an exonuclease from Arabidopsis thaliana reveals similarities to the DNA exonuclease of the human Werner syndrome protein

scientific article published on 22 August 2003

Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana

scientific article published on 17 June 2014

Breaking DNA in plants: how I almost missed my personal breakthrough

scientific article published on 10 June 2015

Breaking news: plants mutate right on target

scientific article

Broadening the applicability of CRISPR/Cas9 in plants

scientific article published on 27 December 2017

CRISPR-Cas9-mediated induction of heritable chromosomal translocations in Arabidopsis

scientific article published on 25 May 2020

CRISPR/Cas-Mediated In Planta Gene Targeting

scientific article published in January 2017

CRISPR/Cas-Mediated Site-Specific Mutagenesis in Arabidopsis thaliana Using Cas9 Nucleases and Paired Nickases

scientific article published in January 2016

CRISPR/Cas-mediated gene targeting in plants: finally a turn for the better for homologous recombination

scientific article published on 23 January 2019

Capture of genomic and T-DNA sequences during double-strand break repair in somatic plant cells

scientific article

Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering

scientific article published on 04 September 2020

Chromatin and development: a special issue

scientific article published in July 2015

DNA Break Repair in Plants and Its Application for Genome Engineering

article

DNA Helicases as Safekeepers of Genome Stability in Plants

scientific article published on 10 December 2019

DNA recombination in somatic plant cells: mechanisms and evolutionary consequences

scientific article published on June 2014

DNA repair meets climate change

scientific article published on 16 November 2020

DNA- and DNA-Protein-Crosslink Repair in Plants ⬇️

scientific article published on 03 September 2019

Defining the roles of the N-terminal region and the helicase activity of RECQ4A in DNA repair and homologous recombination in Arabidopsis

scientific article published on 29 October 2013

Development of Bag-1L as a therapeutic target in androgen receptor-dependent prostate cancer

scientific article published on 10 August 2017

Differences in the processing of DNA ends in Arabidopsis thaliana and tobacco: possible implications for genome evolution

scientific article published on 01 March 2003

Different functions for the domains of the Arabidopsis thaliana RMI1 protein in DNA cross-link repair, somatic and meiotic recombination

scientific article published on 16 August 2013

Different pathways of homologous recombination are used for the repair of double-strand breaks within tandemly arranged sequences in the plant genome

article

Efficient Agrobacterium-mediated transformation of Arabidopsis thaliana using the bar gene as selectable marker.

scientific article published in April 1995

Efficient gene targeting in Nicotiana tabacum using CRISPR/SaCas9 and temperature tolerant LbCas12a

scientific article published on 28 January 2021

Efficient in planta gene targeting in Arabidopsis using egg cell-specific expression of the Cas9 nuclease of Staphylococcus aureus

scientific article published on 17 April 2018

Efficient induction of heritable inversions in plant genomes using the CRISPR/Cas system

article

Efficient repair of genomic double-strand breaks by homologous recombination between directly repeated sequences in the plant genome

article

Elevated UV-B radiation reduces genome stability in plants.

scientific article published in July 2000

Elimination of selection markers from transgenic plants

scientific article

Emerging tools for synthetic biology in plants

article

Endogenous sequence patterns predispose the repair modes of CRISPR/Cas9-induced DNA double-stranded breaks in Arabidopsis thaliana.

scientific article

Engineering CRISPR/LbCas12a for highly efficient, temperature-tolerant plant gene editing

scientific article published on 31 October 2019

Enhancing in planta gene targeting efficiencies in Arabidopsis using temperature-tolerant CRISPR/LbCas12a

scientific article published on 30 May 2020

Fork sensing and strand switching control antagonistic activities of RecQ helicases

scientific article published in January 2013

From classical mutagenesis to nuclease-based breeding - directing natural DNA repair for a natural end-product

scientific article

Gene regulation in response to DNA damage

scientific article published on 16 August 2011

Gene replacement by homologous recombination in plants

scientific article

Gene targeting in plants: 25 years later

scientific article

Gene therapy in plants

scientific article

Genome engineering using CRISPR/Cas: getting more versatile and more precise at the same time

scientific article published on 17 March 2016

Green light for gene targeting in plants ⬇️

scientific article

Highly efficient heritable plant genome engineering using Cas9 orthologues from Streptococcus thermophilus and Staphylococcus aureus

scientific article published on 18 November 2015

Homologous recombination in plant cells is enhanced by in vivo induction of double strand breaks into DNA by a site-specific endonuclease

scientific article published on November 1993

Homology-based double-strand break-induced genome engineering in plants

scientific article published on 15 April 2016

In planta gene targeting

scientific article

In planta gene targeting can be enhanced by the use of CRISPR/Cas12a

scientific article published on 19 September 2019

In planta somatic homologous recombination assay revisited: a successful and versatile, but delicate tool

scientific article published on 9 November 2012

Intron gain and loss in the evolution of the conserved eukaryotic recombination machinery.

scientific article published on December 2002

Involvement of the Cohesin Cofactor PDS5 (SPO76) During Meiosis and DNA Repair in Arabidopsis thaliana

scientific article

Knocking out consumer concerns and regulator's rules: efficient use of CRISPR/Cas ribonucleoprotein complexes for genome editing in cereals

scientific article published on 28 February 2017

Live-cell CRISPR imaging in plants reveals dynamic telomere movements.

scientific article published on 16 May 2017

MHF1 plays Fanconi anaemia complementation group M protein (FANCM)-dependent and FANCM-independent roles in DNA repair and homologous recombination in plants

scientific article

Metal-mediated DNA assembly using the ethynyl linked terpyridine ligand

scientific article published on 16 November 2011

Molecular and biological properties of a cloned and infectious new sequence variant of cucumber pale fruit viroid (CPFV).

scientific article published on August 1988

Molecular characterization of homologues of both subunits A (SPO11) and B of the archaebacterial topoisomerase 6 in plants

scientific article

Nucleotide sequence and secondary structure of apple scar skin viroid (ASSVd) from China

scientific article published in June 1990

Nucleotide sequence of a hop stunt viroid (HSVd) isolate from grapefruit in Israel

scientific article published on February 1989

Nucleotide sequence of a hop stunt viroid (HSVd) isolate from the German grapevine rootstock 5BB as determined by PCR-mediated sequence analysis.

scientific article published on July 1989

Nucleotide sequence of a hop stunt viroid isolate from the German grapevine cultivar 'Riesling'.

scientific article published on March 1988

Nucleotide sequence of the Korean strain of hop stunt viroid (HSV).

scientific article published on September 1988

Primary and secondary structure of citrus viroid IV (CVd IV), a new chimeric viroid present in dwarfed grapefruit in Israel

scientific article published on December 1991

Purification and characterization of RecQ helicases of plants

scientific article published on 01 January 2010

RAD5A, RECQ4A, and MUS81 have specific functions in homologous recombination and define different pathways of DNA repair in Arabidopsis thaliana

scientific article published on 22 October 2010

RecA stimulates sister chromatid exchange and the fidelity of double-strand break repair, but not gene targeting, in plants transformed by Agrobacterium

scientific article

Removing selectable marker genes: taking the shortcut

scientific article published on 01 July 2000

Repair of adjacent single-strand breaks is often accompanied by the formation of tandem sequence duplications in plant genomes

scientific article published on 15 June 2016

Revolutionizing plant biology: multiple ways of genome engineering by CRISPR/Cas

scientific article

Sequence analysis of minute amounts of viroid RNA using the polymerase chain reaction (PCR). Rapid communication

scientific article published on 01 January 1989

Somatic intrachromosomal homologous recombination events in populations of plant siblings

scientific article published on 01 May 1995

Some like it sticky: targeting of the rice gene Waxy

scientific article published on 01 February 2003

Synthetic nucleases for genome engineering in plants: prospects for a bright future

scientific article

The Arabidopsis thaliana homolog of the helicase RTEL1 plays multiple roles in preserving genome stability

scientific article published on 16 December 2014

The CRISPR/Cas revolution continues: From efficient gene editing for crop breeding to plant synthetic biology

scientific article published on 01 December 2018

The CRISPR/Cas revolution reaches the RNA world: Cas13, a new Swiss Army knife for plant biologists

scientific article published on 25 March 2018

The CRISPR/Cas system can be used as nuclease for in planta gene targeting and as paired nickases for directed mutagenesis in Arabidopsis resulting in heritable progeny

scientific article published on 11 November 2014

The DNA translocase RAD5A acts independently of the other main DNA repair pathways, and requires both its ATPase and RING domain for activity in Arabidopsis thaliana

scientific article

The Fanconi anemia ortholog FANCM ensures ordered homologous recombination in both somatic and meiotic cells in Arabidopsis

scientific article

The Protease WSS1A, the Endonuclease MUS81, and the Phosphodiesterase TDP1 Are Involved in Independent Pathways of DNA-protein Crosslink Repair in Plants

scientific article published on 13 February 2019

The RTR Complex Partner RMI2 and the DNA Helicase RTEL1 Are Both Independently Involved in Preserving the Stability of 45S rDNA Repeats in Arabidopsis thaliana

scientific article

The RTR complex as caretaker of genome stability and its unique meiotic function in plants

scientific article published on 12 February 2014

The Rad17 homologue of Arabidopsis is involved in the regulation of DNA damage repair and homologous recombination.

scientific article published in June 2004

The RecQ gene family in plants

scientific article

The RecQ-like helicase HRQ1 is involved in DNA crosslink repair in Arabidopsis in a common pathway with the Fanconi anemia-associated nuclease FAN1 and the postreplicative repair ATPase RAD5A.

scientific article published on 25 March 2018

The STRUCTURAL MAINTENANCE OF CHROMOSOMES 5/6 complex promotes sister chromatid alignment and homologous recombination after DNA damage in Arabidopsis thaliana

scientific article published on 8 September 2009

The Translesion Polymerase ζ Has Roles Dependent on and Independent of the Nuclease MUS81 and the Helicase RECQ4A in DNA Damage Repair in Arabidopsis.

scientific article

The catalytically active tyrosine residues of both SPO11-1 and SPO11-2 are required for meiotic double-strand break induction in Arabidopsis

scientific article published on 26 October 2007

The mechanism of extrachromosomal homologous DNA recombination in plant cells

article

The molecular structure of hop latent viroid (HLV), a new viroid occurring worldwide in hops

scientific article published on May 1988

The nuclease FAN1 is involved in DNA crosslink repair in Arabidopsis thaliana independently of the nuclease MUS81.

scientific article published on 16 March 2015

The repair of double-strand breaks in plants: mechanisms and consequences for genome evolution

scientific article

The role of AtMUS81 in DNA repair and its genetic interaction with the helicase AtRecQ4A.

scientific article published on 31 August 2006

The role of DNA helicases and their interaction partners in genome stability and meiotic recombination in plants ⬇️

scientific article published on November 16, 2010

The role of double-strand break-induced allelic homologous recombination in somatic plant cells

article

The topoisomerase 3α zinc-finger domain T1 of Arabidopsis thaliana is required for targeting the enzyme activity to Holliday junction-like DNA repair intermediates

scientific article published on 17 September 2018

The transcriptional response of Arabidopsis to genotoxic stress - a high-density colony array study (HDCA).

scientific article

Towards CRISPR/Cas crops - bringing together genomics and genome editing.

scientific article

Towards the ideal GMP: homologous recombination and marker gene excision

scientific article

Transforming plant biology and breeding with CRISPR/Cas9, Cas12 and Cas13.

scientific article

Two closely related RecQ helicases have antagonistic roles in homologous recombination and DNA repair in Arabidopsis thaliana

scholarly article

Two different but related mechanisms are used in plants for the repair of genomic double-strand breaks by homologous recombination

scientific article published on May 1996

Two distinct MUS81-EME1 complexes from Arabidopsis process Holliday junctions

scientific article

Two unlinked double-strand breaks can induce reciprocal exchanges in plant genomes via homologous recombination and nonhomologous end joining

scientific article published on 22 October 2006

Use of the Cas9 Orthologs from Streptococcus thermophilus and Staphylococcus aureus for Non-Homologous End-Joining Mediated Site-Specific Mutagenesis in Arabidopsis thaliana

scientific article

Using CRISPR/Cas in three dimensions: towards synthetic plant genomes, transcriptomes and epigenomes

scientific article published on 17 December 2015

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