List of works - Giuseppe Legname

A Fluorescent Styrylquinoline with Combined Therapeutic and Diagnostic Activities against Alzheimer's and Prion Diseases

scientific article

A change in the conformation of prions accompanies the emergence of a new prion strain.

scientific article

A novel class of potential prion drugs: preliminary in vitro and in vivo data for multilayer coated gold nanoparticles

scientific article

A novel expression system for production of soluble prion proteins in E. coli

scientific article published on 10 January 2012

A small chemical library of 2-aminoimidazole derivatives as BACE-1 inhibitors: Structure-based design, synthesis, and biological evaluation

scientific article

A system-level approach for deciphering the transcriptional response to prion infection

scientific article

Aberrant ERK 1/2 complex activation and localization in scrapie-infected GT1-1 cells

scientific article published on 9 August 2010

Aged PrP null mice show defective processing of neuregulins in the peripheral nervous system.

scientific article

Anti-CD30 immunotoxins with native and recombinant dianthin 30.

scientific article published on February 1995

Antibodies inhibit prion propagation and clear cell cultures of prion infectivity

scientific article published in August 2001

Approaches for discovering anti-prion compounds: lessons learned and challenges ahead

scientific article

Binding of neural cell adhesion molecules (N-CAMs) to the cellular prion protein

scientific article

Brain delivery of AAV9 expressing an anti-PrP monovalent antibody delays prion disease in mice.

scientific article

Characterization of four new monoclonal antibodies against the distal N-terminal region of PrP(c).

scientific article

Clinical and neuropathological phenotype associated with the novel V189I mutation in the prion protein gene

article

Cochaperonins Are Histone-Binding Proteins

scientific article published on 01 January 1995

Combining in-situ proteolysis and microseed matrix screening to promote crystallization of PrPc-nanobody complexes

scientific article published on 01 May 2011

Common Structural Traits across Pathogenic Mutants of the Human Prion Protein and Their Implications for Familial Prion Diseases

Compact conformations of α-synuclein induced by alcohols and copper

scientific article published on 01 February 2011

Conformation of PrP(C) on the cell surface as probed by antibodies

scientific article published in February 2003

Continuous quinacrine treatment results in the formation of drug-resistant prions

scientific article published in November 2009

Continuum of prion protein structures enciphers a multitude of prion isolate-specified phenotypes.

scholarly article

Cooperative binding of dominant-negative prion protein to kringle domains

scientific article

Copper coordination in the full-length, recombinant prion protein

scientific article published on June 2003

Copper-catalyzed oxidation of the recombinant SHa(29-231) prion protein

scientific article

Crystallization and preliminary X-ray diffraction analysis of a specific VHH domain against mouse prion protein

scientific article published on 26 November 2010

De novo prions

scientific article

Defined α-synuclein prion-like molecular assemblies spreading in cell culture

scientific article

Design and construction of diverse mammalian prion strains

scientific article

Development of antibody fragments for immunotherapy of prion diseases

scientific article published in March 2009

Developmental influence of the cellular prion protein on the gene expression profile in mouse hippocampus

scientific article published on 15 March 2011

Differential overexpression of SERPINA3 in human prion diseases

scientific article published on 15 November 2017

Discovery of a class of diketopiperazines as antiprion compounds.

scientific article

Docking Ligands on Protein Surfaces: The Case Study of Prion Protein

Dominant-negative effects in prion diseases: insights from molecular dynamics simulations on mouse prion protein chimeras

scientific article published on 30 August 2012

Doppel-induced cerebellar degeneration in transgenic mice ⬇️

scientific article published on December 4, 2001

Early structural features in mammalian prion conformation conversion ⬇️

scientific article published on January 1, 2012

Effects of peptidyl-prolyl isomerase 1 depletion in animal models of prion diseases.

scientific article published in March 2018

Effects of the pathological Q212P mutation on human prion protein non-octarepeat copper-binding site

scientific article published on 27 July 2012

Elucidating the function of the prion protein

scientific article published on 31 August 2017

Epitope mapping of a PrP(Sc)-specific monoclonal antibody: identification of a novel C-terminally truncated prion fragment

scientific article published on 19 December 2010

Evidence for GroES acting as a transcriptional regulator

scientific journal article

Expression and activity of pre-dianthin 30 and dianthin 30.

scientific article published on May 1993

Expression in Escherichia coli, purification and functional activity of recombinant human chaperonin 10

scientific article

Folding of prion protein to its native alpha-helical conformation is under kinetic control

scientific article

Functionalized gold nanoparticles: a detailed in vivo multimodal microscopic brain distribution study

scientific article

Gene expression profiling and therapeutic interventions in neurodegenerative diseases: a comprehensive study on potentiality and limits ⬇️

scientific article published on February 13, 2012

Gene expression profiling of brains from bovine spongiform encephalopathy (BSE)-infected cynomolgus macaques

scientific article

Gene expression profiling to identify druggable targets in prion diseases.

scientific article published on 09 January 2010

Genetic grafting of membrane-acting peptides to the cytotoxin dianthin augments its ability to de-stabilize lipid bilayers and enhances its cytotoxic potential as the component of transferrin-toxin conjugates

scientific article published on 01 May 2000

Hemoglobin mRNA Changes in the Frontal Cortex of Patients with Neurodegenerative Diseases

scientific article published on 22 January 2018

Human prions and plasma lipoproteins

scholarly article

Hybrid lipoic acid derivatives to attack prion disease on multiple fronts

scientific article published on 15 March 2011

Identification and cloning of human chaperonin 10 homologue

scientific article

Identification of two prion protein regions that modify scrapie incubation time

scientific article

Immobilized prion protein undergoes spontaneous rearrangement to a conformation having features in common with the infectious form

scientific article

Immunoglobulins in urine of hamsters with scrapie

scientific article published on 12 August 2004

In vitro aggregation assays for the characterization of α-synuclein prion-like properties.

scientific article published on January 2014

Infrared microspectroscopy: a multiple-screening platform for investigating single-cell biochemical perturbations upon prion infection.

scientific article published on 11 January 2011

Investigating the conformational stability of prion strains through a kinetic replication model

scientific article

Involvement of PrP(C) in kainate-induced excitotoxicity in several mouse strains

scientific article

Iron-mediated interaction of alpha synuclein with lipid raft model membranes

scientific article published on 27 February 2020

Locally disordered conformer of the hamster prion protein: a crucial intermediate to PrPSc?

scientific article published in October 2002

Long-Term Survival But Impaired Homeostatic Proliferation of Naive T Cells in the Absence of p56lck

scientific article published in Science

Mapping the prion protein distribution in marsupials: insights from comparing opossum with mouse CNS

scientific article

Measuring prions causing bovine spongiform encephalopathy or chronic wasting disease by immunoassays and transgenic mice

scientific article

Methionine oxidation in α-synuclein inhibits its propensity for ordered secondary structure

scientific article published on 12 February 2019

Microglia convert aggregated amyloid-β into neurotoxic forms through the shedding of microvesicles.

scientific article published on 13 December 2013

Modulation of alpha-synuclein aggregation by dopamine analogs

scientific article

Mycobacterial Cpn10 promotes recognition of the mammalian homologue by a mycobacterium-specific antiserum

scientific article published in June 1998

NMR Structure of the Human Prion Protein with the Pathological Q212P Mutation Reveals Unique Structural Features

scientific article

Neurodevelopmental expression and localization of the cellular prion protein in the central nervous system of the mouse

scientific article published in June 2010

Nucleotide sequence of cDNA coding for dianthin 30, a ribosome inactivating protein from Dianthus caryophyllus

scientific article

Oriented immobilization of prion protein demonstrated via precise interfacial nanostructure measurements

scientific article published on 19 October 2010

Oxidation of methionine residues in the prion protein by hydrogen peroxide

scientific article published in December 2004

Parallel synthesis, evaluation, and preliminary structure-activity relationship of 2,5-diamino-1,4-benzoquinones as a novel class of bivalent anti-prion compound

scientific article

Pathway complexity of prion protein assembly into amyloid

scientific article published on 23 March 2002

PrPC controls via protein kinase A the direction of synaptic plasticity in the immature hippocampus.

scientific article

Prion and doppel proteins bind to granule cells of the cerebellum

scientific article

Prion detection by an amyloid seeding assay.

scholarly article

Prion protein accumulation in lipid rafts of mouse aging brain

scientific article

Prion protein and copper cooperatively protect neurons by modulating NMDA receptor through S-nitrosylation.

scientific article

Prion protein interaction with soil humic substances: environmental implications

scientific article (publication date: 2014)

Prion protein paralog doppel protein interacts with alpha-2-macroglobulin: a plausible mechanism for doppel-mediated neurodegeneration

scientific article

Prion protein-specific antibodies-development, modes of action and therapeutics application

scientific article

Prion proteins with pathogenic and protective mutations show similar structure and dynamics

scientific article published on September 2009

Prions in skeletal muscle

scientific article

Probing early misfolding events in prion protein mutants by NMR spectroscopy.

scientific article published on 07 August 2013

Probing the N-terminal β-sheet conversion in the crystal structure of the human prion protein bound to a nanobody

scientific article

Progress towards structural understanding of infectious sheep PrP-amyloid.

scientific article

Protease-sensitive synthetic prions

scientific article

Quinacrine is mainly metabolized to mono-desethyl quinacrine by CYP3A4/5 and its brain accumulation is limited by P-glycoprotein

scientific article published on 31 March 2006

Recombinant prion protein induces rapid polarization and development of synapses in embryonic rat hippocampal neurons in vitro

scientific article

Role of Prion Disease-Linked Mutations in the Intrinsically Disordered N-Terminal Domain of the Prion Protein

scientific article published on November 2013

SAXS structural study of PrP(Sc) reveals ~11 nm diameter of basic double intertwined fibers

scientific article published on 18 November 2013

Serpin Signatures in Prion and Alzheimer's Diseases

scientific article published on 13 April 2022

Single-chain ribosome inactivating proteins from plants depurinate Escherichia coli 23S ribosomal RNA.

scientific article published in September 1991

Small-molecule theranostic probes: a promising future in neurodegenerative diseases.

scientific article

Spontaneous generation of anchorless prions in transgenic mice

scientific article published on 12 December 2011

Strain-specified characteristics of mouse synthetic prions

scientific article

Structural determinants in prion protein folding and stability.

scientific article published on 2 October 2014

Structural facets of disease-linked human prion protein mutants: a molecular dynamic study

scientific article published in December 2010

Structural insights into alternate aggregated prion protein forms

scientific article published on 28 August 2009

Structure-activity relationship study of prion inhibition by 2-aminopyridine-3,5-dicarbonitrile-based compounds: parallel synthesis, bioactivity, and in vitro pharmacokinetics

scientific article published on January 2007

Substrate recognition by ribosome-inactivating protein studied by molecular modeling and molecular electrostatic potentials

scientific article published on 01 April 1995

Synthesis and evaluation of a library of 2,5-bisdiamino-benzoquinone derivatives as probes to modulate protein-protein interactions in prions

scientific article

Synthetic Prion Selection and Adaptation

scientific article published on 03 August 2018

Synthetic mammalian prions

scientific article

Synthetic prions and other human neurodegenerative proteinopathies.

scientific article

The influence of the src-family kinases, Lck and Fyn, on T cell differentiation, survival and activation

scientific article

The peculiar nature of unfolding of the human prion protein

scientific article published on 6 February 2004

The role of Bax and caspase-3 in doppel-induced apoptosis of cerebellar granule cells.

scientific article

Thioaptamer interactions with prion proteins: sequence-specific and non-specific binding sites

scientific article

Use of different RT-QuIC substrates for detecting CWD prions in the brain of Norwegian cervids

scientific article published on 09 December 2019

Whole Blood Gene Expression Profiling in Preclinical and Clinical Cattle Infected with Atypical Bovine Spongiform Encephalopathy

scientific article

α-Synuclein Amyloids Hijack Prion Protein to Gain Cell Entry, Facilitate Cell-to-Cell Spreading and Block Prion Replication.

scientific article published on 30 August 2017

α-Synuclein RT-QuIC assay in cerebrospinal fluid of patients with dementia with Lewy bodies

scientific article published on 10 October 2019

List of works by Giuseppe Legname

A Fluorescent Styrylquinoline with Combined Therapeutic and Diagnostic Activities against Alzheimer's and Prion Diseases

A change in the conformation of prions accompanies the emergence of a new prion strain.

A novel class of potential prion drugs: preliminary in vitro and in vivo data for multilayer coated gold nanoparticles

A novel expression system for production of soluble prion proteins in E. coli

A small chemical library of 2-aminoimidazole derivatives as BACE-1 inhibitors: Structure-based design, synthesis, and biological evaluation

A system-level approach for deciphering the transcriptional response to prion infection

Aberrant ERK 1/2 complex activation and localization in scrapie-infected GT1-1 cells

Aged PrP null mice show defective processing of neuregulins in the peripheral nervous system.

Anti-CD30 immunotoxins with native and recombinant dianthin 30.

Antibodies inhibit prion propagation and clear cell cultures of prion infectivity

Approaches for discovering anti-prion compounds: lessons learned and challenges ahead

Binding of neural cell adhesion molecules (N-CAMs) to the cellular prion protein

Brain delivery of AAV9 expressing an anti-PrP monovalent antibody delays prion disease in mice.

Characterization of four new monoclonal antibodies against the distal N-terminal region of PrP(c).

Clinical and neuropathological phenotype associated with the novel V189I mutation in the prion protein gene

Cochaperonins Are Histone-Binding Proteins

Combining in-situ proteolysis and microseed matrix screening to promote crystallization of PrPc-nanobody complexes

Common Structural Traits across Pathogenic Mutants of the Human Prion Protein and Their Implications for Familial Prion Diseases

Compact conformations of α-synuclein induced by alcohols and copper

Conformation of PrP(C) on the cell surface as probed by antibodies

Continuous quinacrine treatment results in the formation of drug-resistant prions

Continuum of prion protein structures enciphers a multitude of prion isolate-specified phenotypes.

Cooperative binding of dominant-negative prion protein to kringle domains

Copper coordination in the full-length, recombinant prion protein

Copper-catalyzed oxidation of the recombinant SHa(29-231) prion protein

Crystallization and preliminary X-ray diffraction analysis of a specific VHH domain against mouse prion protein

De novo prions

Defined α-synuclein prion-like molecular assemblies spreading in cell culture

Design and construction of diverse mammalian prion strains

Development of antibody fragments for immunotherapy of prion diseases

Developmental influence of the cellular prion protein on the gene expression profile in mouse hippocampus

Differential overexpression of SERPINA3 in human prion diseases

Discovery of a class of diketopiperazines as antiprion compounds.

Docking Ligands on Protein Surfaces: The Case Study of Prion Protein

Dominant-negative effects in prion diseases: insights from molecular dynamics simulations on mouse prion protein chimeras

Doppel-induced cerebellar degeneration in transgenic mice ⬇️

Early structural features in mammalian prion conformation conversion ⬇️

Effects of peptidyl-prolyl isomerase 1 depletion in animal models of prion diseases.

Effects of the pathological Q212P mutation on human prion protein non-octarepeat copper-binding site

Elucidating the function of the prion protein

Epitope mapping of a PrP(Sc)-specific monoclonal antibody: identification of a novel C-terminally truncated prion fragment

Evidence for GroES acting as a transcriptional regulator

Expression and activity of pre-dianthin 30 and dianthin 30.

Expression in Escherichia coli, purification and functional activity of recombinant human chaperonin 10

Folding of prion protein to its native alpha-helical conformation is under kinetic control

Functionalized gold nanoparticles: a detailed in vivo multimodal microscopic brain distribution study

Gene expression profiling and therapeutic interventions in neurodegenerative diseases: a comprehensive study on potentiality and limits ⬇️

Gene expression profiling of brains from bovine spongiform encephalopathy (BSE)-infected cynomolgus macaques

Gene expression profiling to identify druggable targets in prion diseases.

Genetic grafting of membrane-acting peptides to the cytotoxin dianthin augments its ability to de-stabilize lipid bilayers and enhances its cytotoxic potential as the component of transferrin-toxin conjugates

Hemoglobin mRNA Changes in the Frontal Cortex of Patients with Neurodegenerative Diseases

Human prions and plasma lipoproteins

Hybrid lipoic acid derivatives to attack prion disease on multiple fronts

Identification and cloning of human chaperonin 10 homologue

Identification of two prion protein regions that modify scrapie incubation time

Immobilized prion protein undergoes spontaneous rearrangement to a conformation having features in common with the infectious form

Immunoglobulins in urine of hamsters with scrapie

In vitro aggregation assays for the characterization of α-synuclein prion-like properties.

Infrared microspectroscopy: a multiple-screening platform for investigating single-cell biochemical perturbations upon prion infection.

Investigating the conformational stability of prion strains through a kinetic replication model

Involvement of PrP(C) in kainate-induced excitotoxicity in several mouse strains

Iron-mediated interaction of alpha synuclein with lipid raft model membranes

Locally disordered conformer of the hamster prion protein: a crucial intermediate to PrPSc?

Long-Term Survival But Impaired Homeostatic Proliferation of Naive T Cells in the Absence of p56lck

Mapping the prion protein distribution in marsupials: insights from comparing opossum with mouse CNS

Measuring prions causing bovine spongiform encephalopathy or chronic wasting disease by immunoassays and transgenic mice

Methionine oxidation in α-synuclein inhibits its propensity for ordered secondary structure

Microglia convert aggregated amyloid-β into neurotoxic forms through the shedding of microvesicles.

Modulation of alpha-synuclein aggregation by dopamine analogs

Mycobacterial Cpn10 promotes recognition of the mammalian homologue by a mycobacterium-specific antiserum

NMR Structure of the Human Prion Protein with the Pathological Q212P Mutation Reveals Unique Structural Features

Neurodevelopmental expression and localization of the cellular prion protein in the central nervous system of the mouse

Nucleotide sequence of cDNA coding for dianthin 30, a ribosome inactivating protein from Dianthus caryophyllus

Oriented immobilization of prion protein demonstrated via precise interfacial nanostructure measurements

Oxidation of methionine residues in the prion protein by hydrogen peroxide

Parallel synthesis, evaluation, and preliminary structure-activity relationship of 2,5-diamino-1,4-benzoquinones as a novel class of bivalent anti-prion compound

Pathway complexity of prion protein assembly into amyloid

PrPC controls via protein kinase A the direction of synaptic plasticity in the immature hippocampus.

Prion and doppel proteins bind to granule cells of the cerebellum