List of works - Karsten Melcher

A D53 repression motif induces oligomerization of TOPLESS corepressors and promotes assembly of a corepressor-nucleosome complex.

scientific article

A Highly Sensitive Non-Radioactive Activity Assay for AMP-Activated Protein Kinase (AMPK)

scientific article published on 13 October 2017

A complex structure of arrestin-2 bound to a G protein-coupled receptor

scientific article published on 27 November 2019

A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors.

scientific article

A highly conserved ATPase protein as a mediator between acidic activation domains and the TATA-binding protein.

scientific article

A modular set of prokaryotic and eukaryotic expression vectors

scientific article

A structural snapshot of the rhodopsin-arrestin complex.

scientific article published on 15 October 2015

Abscisic acid perception and signaling: structural mechanisms and applications

scientific article

Abscisic acid signaling: thermal stability shift assays as tool to analyze hormone perception and signal transduction

scientific article

Alzheimer's disease-associated mutations increase amyloid precursor protein resistance to γ-secretase cleavage and the Aβ42/Aβ40 ratio

scientific article published on 23 August 2016

Amyloid beta: structure, biology and structure-based therapeutic development

scientific article

An ABA-mimicking ligand that reduces water loss and promotes drought resistance in plants

scientific article

An intrinsic agonist mechanism for activation of glucagon-like peptide-1 receptor by its extracellular domain.

scientific article

Androgen receptor: structure, role in prostate cancer and drug discovery

scientific article

Assembly and architecture of the Wnt/β-catenin signalosome at the membrane

scientific article

Bioluminescence Resonance Energy Transfer (BRET) Assay for Determination of Molecular Interactions in Living Cells.

scientific article published in November 2017

Catalytic mechanism and kinase interactions of ABA-signaling PP2C phosphatases

scientific article

Conformational heterogeneity of the allosteric drug and metabolite (ADaM) site in AMP-activated protein kinase (AMPK)

scientific article published on 11 September 2018

Cryo-EM structure of an activated VIP1 receptor-G protein complex revealed by a NanoBiT tethering strategy

scientific article published on 17 August 2020

Crystal structure of heliorhodopsin 48C12

scientific article published on 26 December 2019

Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser

scientific article

Crystal structure of the human 5-HT1B serotonin receptor bound to an inverse agonist.

scientific article published on 13 March 2018

Crystal structures of two phytohormone signal-transducing α/β hydrolases: karrikin-signaling KAI2 and strigolactone-signaling DWARF14

scientific article

Crystallization scale purification of α7 nicotinic acetylcholine receptor from mammalian cells using a BacMam expression system

scientific article

DWARF 53 acts as a repressor of strigolactone signalling in rice

scientific article published on 11 December 2013

Deconvoluting AMP-activated protein kinase (AMPK) adenine nucleotide binding and sensing

scientific article published on 14 June 2017

Defining the minimum substrate and charge recognition model of gamma-secretase

scientific article published on 17 April 2017

Destabilization of strigolactone receptor DWARF14 by binding of ligand and E3-ligase signaling effector DWARF3.

scientific article

Detection of Membrane Protein Interactions by Cell-based Tango Assays.

scientific article published in November 2017

Development of highly potent glucocorticoids for steroid-resistant severe asthma

scientific article published on 20 March 2019

Differential Requirement of the Extracellular Domain in Activation of Class B G Protein-coupled Receptors

scientific article published on 13 May 2016

Dimerization of the transmembrane domain of amyloid precursor protein is determined by residues around the γ-secretase cleavage sites.

scientific article published on 8 August 2017

Discovery of a highly potent glucocorticoid for asthma treatment

scientific article published on 15 December 2015

Erratum: Discovery of a highly potent glucocorticoid for asthma treatment.

scientific article published on 26 January 2016

Erratum: structural basis of AMPK regulation by adenine nucleotides and glycogen.

scientific article published in March 2015

Evidence for two modes of cooperative DNA binding in vivo that do not involve direct protein-protein interactions

scientific article published on 01 April 1998

Gal80-Gal80 interaction on adjacent Gal4p binding sites is required for complete GAL gene repression

scientific article

Generation and characterization of a human nanobody against VEGFR-2.

scientific article

Genetic analysis of serine biosynthesis and glucose repression in yeast

scientific article published on April 1, 1992

H2O2 inhibits ABA-signaling protein phosphatase HAB1.

scientific article

High yield and efficient expression and purification of the human 5-HT3A receptor

scientific article

Identification and characterization of the activation domain of Ifh1, an activator of model TATA-less genes

scientific article

Identification and mechanism of ABA receptor antagonism

scientific article

Identification and structural insight of an effective PPARγ modulator with improved therapeutic index for anti-diabetic drug discovery

scientific article published on 21 January 2020

Identification of Phosphorylation Codes for Arrestin Recruitment by G Protein-Coupled Receptors.

scientific article published on July 2017

Identification of SRC3/AIB1 as a Preferred Coactivator for Hormone-activated Androgen Receptor

scientific article

Identification of a novel selective PPARγ ligand with a unique binding mode and improved therapeutic profile in vitro.

scientific article published on 27 January 2017

Ion channels gated by acetylcholine and serotonin: structures, biology, and drug discovery

scientific article (publication date: August 2015)

Long-chain fatty acyl-CoA esters regulate metabolism via allosteric control of AMPK β1 isoforms

scientific article published on 27 July 2020

Molecular Mimicry Regulates ABA Signaling by SnRK2 Kinases and PP2C Phosphatases

scientific article

Molecular analysis of the yeast SER1 gene encoding 3-phosphoserine aminotransferase: regulation by general control and serine repression

scientific article published on May 1995

Molecular assembly of rhodopsin with G protein-coupled receptor kinases.

scientific article

Molecular mechanisms of fentanyl mediated β-arrestin biased signaling

scientific article published on 10 April 2020

Mutational hypersensitivity of a gene regulatory protein: Saccharomyces cerevisiae Gal80p

scientific article

New chemical crosslinking methods for the identification of transient protein-protein interactions with multiprotein complexes.

scientific article published in August 2004

New chemistry for the study of multiprotein complexes: the six-histidine tag as a receptor for a protein crosslinking reagent

scientific article published on 01 July 1996

Protein conformation ensembles monitored by HDX reveal a structural rationale for abscisic acid signaling protein affinities and activities

scientific article published on 03 January 2013

Rearrangement of a polar core provides a conserved mechanism for constitutive activation of class B G protein-coupled receptors ⬇️

scientific article

Streptavidin Bead Pulldown Assay to Determine Protein Homooligomerization.

scientific article published in November 2017

Structural Basis for Molecular Recognition at Serotonin Receptors

scientific article

Structural Basis of TPR-Mediated Oligomerization and Activation of Oncogenic Fusion Kinases.

scientific article published on 18 May 2017

Structural Conservation of Ligand Binding Reveals a Bile Acid-like Signaling Pathway in Nematodes

scientific article

Structural and functional study of D-glucuronyl C5-epimerase

scientific article

Structural basis for RNA recognition by a dimeric PPR-protein complex

scientific article

Structural basis for basal activity and autoactivation of abscisic acid (ABA) signaling SnRK2 kinases

scientific article

Structural basis for corepressor assembly by the orphan nuclear receptor TLX.

scientific article

Structural basis for molecular recognition of folic acid by folate receptors

scientific article

Structural basis for recognition of diverse transcriptional repressors by the TOPLESS family of corepressors.

scientific article

Structural basis of AMPK regulation by adenine nucleotides and glycogen.

scientific article

Structural basis of Fusarium myosin I inhibition by phenamacril

scientific article published on 12 March 2020

Structural basis of JAZ repression of MYC transcription factors in jasmonate signalling

scientific article

Structural basis of binding and inhibition of ornithine decarboxylase by 1-amino-oxy-3-aminopropane

scientific article published on 01 December 2021

Structural biology: When sperm meets egg.

scientific article published on 15 June 2016

Structural insights into alternative splicing-mediated desensitization of jasmonate signaling.

scientific article

Structural insights into gene repression by the orphan nuclear receptor SHP

scientific article

Structural mechanisms of RNA recognition: sequence-specific and non-specific RNA-binding proteins and the Cas9-RNA-DNA complex

scientific article

Structure and Physiological Regulation of AMPK ⬇️

scientific article published on 09 November 2018

Structure and activation of rhodopsin

scientific article published on 23 January 2012

Structure and dynamics of the active human parathyroid hormone receptor-1

scientific article

Structure and function of Norrin in assembly and activation of a Frizzled 4-Lrp5/6 complex

scientific article

Structure and mechanism for recognition of peptide hormones by Class B G-protein-coupled receptors

scientific article

Structure modeling using genetically engineered crosslinking

scientific article published on 01 December 2013

Structure of a PLS-class Pentatricopeptide Repeat Protein Provides Insights into Mechanism of RNA Recognition

scientific article

Structure of formylpeptide receptor 2-Gi complex reveals insights into ligand recognition and signaling

scientific article published on 14 February 2020

Structure of nucleosome-bound DNA methyltransferases DNMT3A and DNMT3B

scientific article published on 23 September 2020

Structure of the PRC2 complex and application to drug discovery.

scientific article

Structures and mechanism for the design of highly potent glucocorticoids

scientific article

Structures and regulation of non-X orphan nuclear receptors: A retinoid hypothesis

scientific article published on 06 July 2015

Structures of AMP-activated protein kinase bound to novel pharmacological activators in phosphorylated, non-phosphorylated, and nucleotide-free states

scientific article published on 26 November 2018

The Crystal Structure of the Orphan Nuclear Receptor NR2E3/PNR Ligand Binding Domain Reveals a Dimeric Auto-Repressed Conformation

scientific article

The cryo-electron microscopy structure of γ-Secretase: towards complex assembly, substrate recognition and a catalytic mechanism

scientific article published on March 2015

The potent Cdc7-Dbf4 (DDK) kinase inhibitor XL413 has limited activity in many cancer cell lines and discovery of potential new DDK inhibitor scaffolds

scientific article

The strength of acidic activation domains correlates with their affinity for both transcriptional and non-transcriptional proteins

scientific article

The structural basis of the dominant negative phenotype of the Gαi1β1γ2 G203A/A326S heterotrimer.

scientific article published on 08 August 2016

Thirsty plants and beyond: structural mechanisms of abscisic acid perception and signaling

scientific article

Tumor Targeting with Novel 6-Substituted Pyrrolo [2,3-d] Pyrimidine Antifolates with Heteroatom Bridge Substitutions via Cellular Uptake by Folate Receptor α and the Proton-Coupled Folate Transporter and Inhibition of de Novo Purine Nucleotide Biosy

scientific article published on 26 July 2016

Understanding the GPCR biased signaling through G protein and arrestin complex structures.

scientific article

Upregulation of glycans containing 3' fucose in a subset of pancreatic cancers uncovered using fusion-tagged lectins.

scientific article published on 12 May 2015

Use of a genetically introduced cross-linker to identify interaction sites of acidic activators within native transcription factor IID and SAGA.

scientific article

Wnt5a promotes Frizzled-4 signalosome assembly by stabilizing cysteine-rich domain dimerization

scientific article published on 25 May 2017

X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex

scientific article

Zero background yeast reporter plasmids

scientific article published on April 2000

γ-Secretase Epsilon-cleavage Assay

scientific article published in November 2017

List of works by Karsten Melcher

A D53 repression motif induces oligomerization of TOPLESS corepressors and promotes assembly of a corepressor-nucleosome complex.

A Highly Sensitive Non-Radioactive Activity Assay for AMP-Activated Protein Kinase (AMPK)

A complex structure of arrestin-2 bound to a G protein-coupled receptor

A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors.

A highly conserved ATPase protein as a mediator between acidic activation domains and the TATA-binding protein.

A modular set of prokaryotic and eukaryotic expression vectors

A structural snapshot of the rhodopsin-arrestin complex.

Abscisic acid perception and signaling: structural mechanisms and applications

Abscisic acid signaling: thermal stability shift assays as tool to analyze hormone perception and signal transduction

Alzheimer's disease-associated mutations increase amyloid precursor protein resistance to γ-secretase cleavage and the Aβ42/Aβ40 ratio

Amyloid beta: structure, biology and structure-based therapeutic development

An ABA-mimicking ligand that reduces water loss and promotes drought resistance in plants

An intrinsic agonist mechanism for activation of glucagon-like peptide-1 receptor by its extracellular domain.

Androgen receptor: structure, role in prostate cancer and drug discovery

Assembly and architecture of the Wnt/β-catenin signalosome at the membrane

Bioluminescence Resonance Energy Transfer (BRET) Assay for Determination of Molecular Interactions in Living Cells.

Catalytic mechanism and kinase interactions of ABA-signaling PP2C phosphatases

Conformational heterogeneity of the allosteric drug and metabolite (ADaM) site in AMP-activated protein kinase (AMPK)

Cryo-EM structure of an activated VIP1 receptor-G protein complex revealed by a NanoBiT tethering strategy

Crystal structure of heliorhodopsin 48C12

Crystal structure of rhodopsin bound to arrestin by femtosecond X-ray laser

Crystal structure of the human 5-HT1B serotonin receptor bound to an inverse agonist.

Crystal structures of two phytohormone signal-transducing α/β hydrolases: karrikin-signaling KAI2 and strigolactone-signaling DWARF14

Crystallization scale purification of α7 nicotinic acetylcholine receptor from mammalian cells using a BacMam expression system

DWARF 53 acts as a repressor of strigolactone signalling in rice

Deconvoluting AMP-activated protein kinase (AMPK) adenine nucleotide binding and sensing

Defining the minimum substrate and charge recognition model of gamma-secretase

Destabilization of strigolactone receptor DWARF14 by binding of ligand and E3-ligase signaling effector DWARF3.

Detection of Membrane Protein Interactions by Cell-based Tango Assays.

Development of highly potent glucocorticoids for steroid-resistant severe asthma

Differential Requirement of the Extracellular Domain in Activation of Class B G Protein-coupled Receptors

Dimerization of the transmembrane domain of amyloid precursor protein is determined by residues around the γ-secretase cleavage sites.

Discovery of a highly potent glucocorticoid for asthma treatment

Erratum: Discovery of a highly potent glucocorticoid for asthma treatment.

Erratum: structural basis of AMPK regulation by adenine nucleotides and glycogen.

Evidence for two modes of cooperative DNA binding in vivo that do not involve direct protein-protein interactions

Gal80-Gal80 interaction on adjacent Gal4p binding sites is required for complete GAL gene repression

Generation and characterization of a human nanobody against VEGFR-2.

Genetic analysis of serine biosynthesis and glucose repression in yeast

H2O2 inhibits ABA-signaling protein phosphatase HAB1.

High yield and efficient expression and purification of the human 5-HT3A receptor

Identification and characterization of the activation domain of Ifh1, an activator of model TATA-less genes

Identification and mechanism of ABA receptor antagonism

Identification and structural insight of an effective PPARγ modulator with improved therapeutic index for anti-diabetic drug discovery

Identification of Phosphorylation Codes for Arrestin Recruitment by G Protein-Coupled Receptors.

Identification of SRC3/AIB1 as a Preferred Coactivator for Hormone-activated Androgen Receptor

Identification of a novel selective PPARγ ligand with a unique binding mode and improved therapeutic profile in vitro.

Ion channels gated by acetylcholine and serotonin: structures, biology, and drug discovery

Long-chain fatty acyl-CoA esters regulate metabolism via allosteric control of AMPK β1 isoforms

Molecular Mimicry Regulates ABA Signaling by SnRK2 Kinases and PP2C Phosphatases

Molecular analysis of the yeast SER1 gene encoding 3-phosphoserine aminotransferase: regulation by general control and serine repression

Molecular assembly of rhodopsin with G protein-coupled receptor kinases.

Molecular mechanisms of fentanyl mediated β-arrestin biased signaling

Mutational hypersensitivity of a gene regulatory protein: Saccharomyces cerevisiae Gal80p

New chemical crosslinking methods for the identification of transient protein-protein interactions with multiprotein complexes.

New chemistry for the study of multiprotein complexes: the six-histidine tag as a receptor for a protein crosslinking reagent

Protein conformation ensembles monitored by HDX reveal a structural rationale for abscisic acid signaling protein affinities and activities

Rearrangement of a polar core provides a conserved mechanism for constitutive activation of class B G protein-coupled receptors ⬇️

Streptavidin Bead Pulldown Assay to Determine Protein Homooligomerization.

Structural Basis for Molecular Recognition at Serotonin Receptors

Structural Basis of TPR-Mediated Oligomerization and Activation of Oncogenic Fusion Kinases.

Structural Conservation of Ligand Binding Reveals a Bile Acid-like Signaling Pathway in Nematodes

Structural and functional study of D-glucuronyl C5-epimerase

Structural basis for RNA recognition by a dimeric PPR-protein complex

Structural basis for basal activity and autoactivation of abscisic acid (ABA) signaling SnRK2 kinases

Structural basis for corepressor assembly by the orphan nuclear receptor TLX.

Structural basis for molecular recognition of folic acid by folate receptors

Structural basis for recognition of diverse transcriptional repressors by the TOPLESS family of corepressors.

Structural basis of AMPK regulation by adenine nucleotides and glycogen.

Structural basis of Fusarium myosin I inhibition by phenamacril

Structural basis of JAZ repression of MYC transcription factors in jasmonate signalling

Structural basis of binding and inhibition of ornithine decarboxylase by 1-amino-oxy-3-aminopropane

Structural biology: When sperm meets egg.

Structural insights into alternative splicing-mediated desensitization of jasmonate signaling.

Structural insights into gene repression by the orphan nuclear receptor SHP

Structural mechanisms of RNA recognition: sequence-specific and non-specific RNA-binding proteins and the Cas9-RNA-DNA complex

Structure and Physiological Regulation of AMPK ⬇️

Structure and activation of rhodopsin

Structure and dynamics of the active human parathyroid hormone receptor-1