List of works - Gianfranco Gilardi

A New Standardized Electrochemical Array for Drug Metabolic Profiling with Human Cytochromes P450

scientific article published on 15 May 2011

A rapid screening for cytochrome P450 catalysis on new chemical entities: cytochrome P450 BM3 and 1,2,5-oxadiazole derivatives

scientific article published on 13 September 2012

An electrochemical microfluidic platform for human P450 drug metabolism profiling.

scientific article published on 24 November 2010

Atypical effect of temperature tuning on the insertion of the catalytic iron-sulfur center in a recombinant [FeFe]-hydrogenase.

scientific article published on 12 September 2015

Backbone dynamics of azurin in solution: slow conformational change associated with deprotonation of histidine 35.

scientific article published in September 1999

Biochemical features of dye-decolorizing peroxidases: current impact on lignin degradation

scientific article published on 29 August 2020

Biohydrogen and biomethane production sustained by untreated matrices and alternative application of compost waste

scientific article published on 12 July 2016

Breakthrough in P450 bioelectrochemistry and future perspectives.

scientific article published on 18 July 2010

CYP116B5: a new class VII catalytically self-sufficient cytochrome P450 from Acinetobacter radioresistens that enables growth on alkanes

scientific article published on 30 December 2014

Catalytic properties of catechol 1,2-dioxygenase from Acinetobacter radioresistens S13 immobilized on nanosponges

scientific article published on 14 July 2009

Characterisation of the electron transfer and complex formation between flavodoxin from D. vulgaris and the haem domain of cytochrome P450 BM3 from B. megaterium.

scientific article published on 30 January 2009

Characterization of a new Baeyer-Villiger monooxygenase and conversion to a solely N-or S-oxidizing enzyme by a single R292 mutation

scientific article published on 22 June 2016

Chimeric P450 enzymes: activity of artificial redox fusions driven by different reductases for biotechnological applications.

scientific article published on January 2013

Chimeric cytochrome P450 3A4 used for in vitro prediction of food-drug interactions

scientific article published on 26 July 2020

Comparison of the refined crystal structures of wild-type (1.34 A) flavodoxin from Desulfovibrio vulgaris and the S35C mutant (1.44 A) at 100 K

scientific article

Control of human cytochrome P450 2E1 electrocatalytic response as a result of unique orientation on gold electrodes.

scientific article published in June 2010

Cyclic voltammetry and voltabsorptometry studies of redox proteins immobilised on nanocrystalline tin dioxide electrodes

scientific article published in June 2004

Direct electrochemistry of an [FeFe]-hydrogenase on a TiO2 Electrode

scientific article published in 2011

Direct electrochemistry of drug metabolizing human flavin-containing monooxygenase: electrochemical turnover of benzydamine and tamoxifen

scientific article published in January 2010

Direct electrochemistry of immobilized human cytochrome P450 2E1.

scientific article published in April 2004

Direct spectroscopic evidence for binding of anastrozole to the iron heme of human aromatase. Peering into the mechanism of aromatase inhibition

scientific article published on 22 August 2011

Directed evolution of enzymes for product chemistry.

scientific article published on 15 July 2004

Drug–drug interactions and cooperative effects detected in electrochemically driven human cytochrome P450 3A4

scientific article published in August 2012

Dynamics and flexibility of human aromatase probed by FTIR and time resolved fluorescence spectroscopy

scientific article

Effect of sildenafil on human aromatase activity: From in vitro structural analysis to catalysis and inhibition in cells

scientific article published on 8 September 2016

Effects of protein-protein interactions on electron transfer: docking and electron transfer calculations for complexes between flavodoxin and c-type cytochromes

scientific article published in July 1999

Electrochemical detection of human cytochrome P450 2A6 inhibition: a step toward reducing dependence on smoking

scientific article published on 14 February 2014

Electrochemistry of Canis familiaris cytochrome P450 2D15 with gold nanoparticles: An alternative to animal testing in drug discovery.

scientific article published on 12 June 2015

Engineering Macaca fascicularis cytochrome P450 2C20 to reduce animal testing for new drugs.

scientific article published on 7 June 2012

Engineering and design in the bioelectrochemistry of metalloproteins.

scientific article

Engineering heme binding sites in monomeric rop.

scientific article published on 17 January 2009

Engineering human cytochrome P450 enzymes into catalytically self-sufficient chimeras using molecular Lego

scientific article published on 22 July 2006

Engineering multi-domain redox proteins containing flavodoxin as bio-transformer: preparatory studies by rational design

scientific article published in September 1998

Engineering type 1 copper sites in proteins.

scientific article

Entrapment of human flavin-containing monooxygenase 3 in the presence of gold nanoparticles: TEM, FTIR and electrocatalysis.

scientific article published on 2 October 2012

Enzyme-Based Amperometric Platform to Determine the Polymorphic Response in Drug Metabolism by Cytochromes P450

scientific article published on 15 March 2011

Escherichia coli Overexpressing a Baeyer-Villiger Monooxygenase from Acinetobacter radioresistens Becomes Resistant to Imipenem.

scientific article

Evidence for an elevated aspartate pK(a) in the active site of human aromatase

scientific article

Flavin-Containing Monooxygenase 3 Polymorphic Variants Significantly Affect Clearance of Tamoxifen and Clomiphene

scientific article published on 02 August 2018

Fluorescence detection of ligand binding to labeled cytochrome P450BM3

scientific article published in 2012

Functional characterisation of an engineered multidomain human P450 2E1 by molecular Lego.

scientific article

Graphene oxide-mediated electrochemistry of glucose oxidase on glassy carbon electrodes.

scientific article published on 14 July 2015

Human Cytochrome P450 3A4 as a Biocatalyst: Effects of the Engineered Linker in Modulation of Coupling Efficiency in 3A4-BMR Chimeras

scientific article published on 21 March 2017

Human aromatase: perspectives in biochemistry and biotechnology.

scientific article published on January 2013

Human cytomegalovirus US21 protein is a viroporin that modulates calcium homeostasis and protects cells against apoptosis

scientific article published on 10 December 2018

Human flavin-containing monooxygenase 3 on graphene oxide for drug metabolism screening.

scientific article

Human flavin-containing monooxygenase 3: Structural mapping of gene polymorphisms and insights into molecular basis of drug binding.

scientific article

Hydrogen production at high Faradaic efficiency by a bio-electrode based on TiO2 adsorption of a new [FeFe]-hydrogenase from Clostridium perfringens.

scientific article published on 11 August 2015

Hydroxylation of non-substituted polycyclic aromatic hydrocarbons by cytochrome P450 BM3 engineered by directed evolution.

scientific article

Identification of a novel Baeyer‐Villiger monooxygenase from Acinetobacter radioresistens: close relationship to the Mycobacterium tuberculosis prodrug activator EtaA ⬇️

scientific article published on August 2, 2012

Identification of endocrine disrupting chemicals acting on human aromatase.

scientific article

Identification of human flavin-containing monooxygenase 3 substrates by a colorimetric screening assay.

scientific article published on 27 January 2017

Identification of mutant Asp251Gly/Gln307His of cytochrome P450 BM3 for the generation of metabolites of diclofenac, ibuprofen and tolbutamide.

scientific article

Impact of R264C and R264H polymorphisms in human aromatase function

scientific article published on October 2016

Improvement of biocatalysts for industrial and environmental purposes by saturation mutagenesis

scientific article

Improving catalytic properties of P450 BM3 haem domain electrodes by molecular Lego

scientific article published on 13 February 2006

In vitro drug metabolism by C-terminally truncated human flavin-containing monooxygenase 3.

scientific article

Inactivation mechanism of N61S mutant of human FMO3 towards trimethylamine.

scientific article published on 7 November 2017

Influence of inter-domain dynamics and surrounding environment flexibility on the direct electrochemistry and electrocatalysis of self-sufficient cytochrome P450 3A4-BMR chimeras

scientific article published in November 2018

Ionic strength dependence of the non-physiological electron transfer between flavodoxin and cytochrome c 553 from D. vulgaris

scientific article published in November 2000

Iron-based redox centres of reductase and oxygenase components of phenol hydroxylase from A. radioresistens: a redox chain working at highly positive redox potentials

scientific article published in 2012

Isolation and characterization of a new [FeFe]-hydrogenase from Clostridium perfringens

scientific article published in July 2015

Laser-written nanoporous silicon diffraction gratings for biosensors

scientific article

Layer-by-Layer Assembly of Supported Lipid Bilayer Poly-L-Lysine Multilayers. ⬇️

scientific article published on 7 December 2015

Mediated electrochemistry of peroxidases—effects of variations in protein and mediator structures

scientific article published in December 1997

Modulation of the interaction between human P450 3A4 and B. megaterium reductase via engineered loops.

scientific article

Molecular Basis for Endocrine Disruption by Pesticides Targeting Aromatase and Estrogen Receptor

scientific article published on 05 August 2020

Molecular Lego: design of molecular assemblies of P450 enzymes for nanobiotechnology

scientific article published in January 2002

Optimization of the bacterial cytochrome P450 BM3 system for the production of human drug metabolites

scientific article

Oxygen Stability in the New [FeFe]-Hydrogenase from Clostridium beijerinckii SM10 (CbA5H).

scientific article published on 17 October 2016

P450 versus P420: correlation between cyclic voltammetry and visible absorption spectroscopy of the immobilized heme domain of cytochrome P450 BM3.

scientific article published on 9 October 2008

P450-based porous silicon biosensor for arachidonic acid detection

scientific article published on 22 July 2011

Peroxide-driven catalysis of the heme domain of A. radioresistens cytochrome P450 116B5 for sustainable aromatic rings oxidation and drug metabolites production

scientific article published on 29 August 2019

Phenol hydroxylase from Acinetobacter radioresistens is a multicomponent enzyme. Purification and characterization of the reductase moiety

scientific article published in October 1999

Protein Adsorption on Nanocrystalline TiO(2) Films: An Immobilization Strategy for Bioanalytical Devices

scientific article

Protein and electrode engineering for the covalent immobilization of P450 BMP on gold.

scientific article

Proton-coupled electron transfer of flavodoxin immobilized on nanostructured tin dioxide electrodes: thermodynamics versus kinetics control of protein redox function

scientific article published in June 2004

Radical intermediates in veratryl alcohol oxidation by ligninase. NMR evidence

scientific article published in November 1990

Redox properties and crystal structures of a Desulfovibrio vulgaris flavodoxin mutant in the monomeric and homodimeric forms

scientific article

Resolution of the heterogeneous fluorescence in multi-tryptophan proteins: ascorbate oxidase.

scientific article

Site saturation mutagenesis demonstrates a central role for cysteine 298 as proton donor to the catalytic site in CaHydA [FeFe]-hydrogenase

scientific article

Structural Basis for the Functional Roles of Critical Residues in Human Cytochrome P450 Aromatase

scientific article

Subtle structural changes in the Asp251Gly/Gln307His P450 BM3 mutant responsible for new activity toward diclofenac, tolbutamide and ibuprofen

scientific article

The Cranberry Extract Oximacro Exerts Virucidal Activity Against Influenza Virus by Interfering With Hemagglutinin

scientific article published on 07 August 2018

The effect of a C298D mutation in CaHydA [FeFe]-hydrogenase: Insights into the protein-metal cluster interaction by EPR and FTIR spectroscopic investigation

scientific article published on 19 October 2015

The effect of pressure and guanidine hydrochloride on azurins mutated in the hydrophobic core

scientific article published in October 1999

The oxygenase component of phenol hydroxylase from Acinetobacter radioresistens S13.

scientific article published in May 2003

Toward reduction in animal sacrifice for drugs: molecular modeling of Macaca fascicularis P450 2C20 for virtual screening of Homo sapiens P450 2C8 substrates

scientific article published in November 2012

Tuning the reduction potential of engineered cytochrome c-553.

scientific article published in July 2002

Turin special issue: Biochemistry for tomorrow's medicine

scientific article published on 6 May 2011

Understanding uncoupling in the multiredox centre P450 3A4–BMR model system

scientific article published on 21 September 2010

Wild-type CYP102A1 as a biocatalyst: turnover of drugs usually metabolised by human liver enzymes

scientific article published on 18 January 2007

Working at the membrane interface: Ligand-induced changes in dynamic conformation and oligomeric structure in human aromatase

scientific article published on 19 September 2017

List of works by Gianfranco Gilardi

A New Standardized Electrochemical Array for Drug Metabolic Profiling with Human Cytochromes P450

A rapid screening for cytochrome P450 catalysis on new chemical entities: cytochrome P450 BM3 and 1,2,5-oxadiazole derivatives

An electrochemical microfluidic platform for human P450 drug metabolism profiling.

Atypical effect of temperature tuning on the insertion of the catalytic iron-sulfur center in a recombinant [FeFe]-hydrogenase.

Backbone dynamics of azurin in solution: slow conformational change associated with deprotonation of histidine 35.

Biochemical features of dye-decolorizing peroxidases: current impact on lignin degradation

Biohydrogen and biomethane production sustained by untreated matrices and alternative application of compost waste

Breakthrough in P450 bioelectrochemistry and future perspectives.

CYP116B5: a new class VII catalytically self-sufficient cytochrome P450 from Acinetobacter radioresistens that enables growth on alkanes

Catalytic properties of catechol 1,2-dioxygenase from Acinetobacter radioresistens S13 immobilized on nanosponges

Characterisation of the electron transfer and complex formation between flavodoxin from D. vulgaris and the haem domain of cytochrome P450 BM3 from B. megaterium.

Characterization of a new Baeyer-Villiger monooxygenase and conversion to a solely N-or S-oxidizing enzyme by a single R292 mutation

Chimeric P450 enzymes: activity of artificial redox fusions driven by different reductases for biotechnological applications.

Chimeric cytochrome P450 3A4 used for in vitro prediction of food-drug interactions

Comparison of the refined crystal structures of wild-type (1.34 A) flavodoxin from Desulfovibrio vulgaris and the S35C mutant (1.44 A) at 100 K

Control of human cytochrome P450 2E1 electrocatalytic response as a result of unique orientation on gold electrodes.

Cyclic voltammetry and voltabsorptometry studies of redox proteins immobilised on nanocrystalline tin dioxide electrodes

Direct electrochemistry of an [FeFe]-hydrogenase on a TiO2 Electrode

Direct electrochemistry of drug metabolizing human flavin-containing monooxygenase: electrochemical turnover of benzydamine and tamoxifen

Direct electrochemistry of immobilized human cytochrome P450 2E1.

Direct spectroscopic evidence for binding of anastrozole to the iron heme of human aromatase. Peering into the mechanism of aromatase inhibition

Directed evolution of enzymes for product chemistry.

Drug–drug interactions and cooperative effects detected in electrochemically driven human cytochrome P450 3A4

Dynamics and flexibility of human aromatase probed by FTIR and time resolved fluorescence spectroscopy

Effect of sildenafil on human aromatase activity: From in vitro structural analysis to catalysis and inhibition in cells

Effects of protein-protein interactions on electron transfer: docking and electron transfer calculations for complexes between flavodoxin and c-type cytochromes

Electrochemical detection of human cytochrome P450 2A6 inhibition: a step toward reducing dependence on smoking

Electrochemistry of Canis familiaris cytochrome P450 2D15 with gold nanoparticles: An alternative to animal testing in drug discovery.

Engineering Macaca fascicularis cytochrome P450 2C20 to reduce animal testing for new drugs.

Engineering and design in the bioelectrochemistry of metalloproteins.

Engineering heme binding sites in monomeric rop.

Engineering human cytochrome P450 enzymes into catalytically self-sufficient chimeras using molecular Lego

Engineering multi-domain redox proteins containing flavodoxin as bio-transformer: preparatory studies by rational design

Engineering type 1 copper sites in proteins.

Entrapment of human flavin-containing monooxygenase 3 in the presence of gold nanoparticles: TEM, FTIR and electrocatalysis.

Enzyme-Based Amperometric Platform to Determine the Polymorphic Response in Drug Metabolism by Cytochromes P450

Escherichia coli Overexpressing a Baeyer-Villiger Monooxygenase from Acinetobacter radioresistens Becomes Resistant to Imipenem.

Evidence for an elevated aspartate pK(a) in the active site of human aromatase

Flavin-Containing Monooxygenase 3 Polymorphic Variants Significantly Affect Clearance of Tamoxifen and Clomiphene

Fluorescence detection of ligand binding to labeled cytochrome P450BM3

Functional characterisation of an engineered multidomain human P450 2E1 by molecular Lego.

Graphene oxide-mediated electrochemistry of glucose oxidase on glassy carbon electrodes.

Human Cytochrome P450 3A4 as a Biocatalyst: Effects of the Engineered Linker in Modulation of Coupling Efficiency in 3A4-BMR Chimeras

Human aromatase: perspectives in biochemistry and biotechnology.

Human cytomegalovirus US21 protein is a viroporin that modulates calcium homeostasis and protects cells against apoptosis

Human flavin-containing monooxygenase 3 on graphene oxide for drug metabolism screening.

Human flavin-containing monooxygenase 3: Structural mapping of gene polymorphisms and insights into molecular basis of drug binding.

Hydrogen production at high Faradaic efficiency by a bio-electrode based on TiO2 adsorption of a new [FeFe]-hydrogenase from Clostridium perfringens.

Hydroxylation of non-substituted polycyclic aromatic hydrocarbons by cytochrome P450 BM3 engineered by directed evolution.

Identification of a novel Baeyer‐Villiger monooxygenase from Acinetobacter radioresistens: close relationship to the Mycobacterium tuberculosis prodrug activator EtaA ⬇️

Identification of endocrine disrupting chemicals acting on human aromatase.

Identification of human flavin-containing monooxygenase 3 substrates by a colorimetric screening assay.

Identification of mutant Asp251Gly/Gln307His of cytochrome P450 BM3 for the generation of metabolites of diclofenac, ibuprofen and tolbutamide.

Impact of R264C and R264H polymorphisms in human aromatase function

Improvement of biocatalysts for industrial and environmental purposes by saturation mutagenesis

Improving catalytic properties of P450 BM3 haem domain electrodes by molecular Lego

In vitro drug metabolism by C-terminally truncated human flavin-containing monooxygenase 3.

Inactivation mechanism of N61S mutant of human FMO3 towards trimethylamine.

Influence of inter-domain dynamics and surrounding environment flexibility on the direct electrochemistry and electrocatalysis of self-sufficient cytochrome P450 3A4-BMR chimeras

Ionic strength dependence of the non-physiological electron transfer between flavodoxin and cytochrome c 553 from D. vulgaris

Iron-based redox centres of reductase and oxygenase components of phenol hydroxylase from A. radioresistens: a redox chain working at highly positive redox potentials

Isolation and characterization of a new [FeFe]-hydrogenase from Clostridium perfringens

Laser-written nanoporous silicon diffraction gratings for biosensors

Layer-by-Layer Assembly of Supported Lipid Bilayer Poly-L-Lysine Multilayers. ⬇️

Mediated electrochemistry of peroxidases—effects of variations in protein and mediator structures

Modulation of the interaction between human P450 3A4 and B. megaterium reductase via engineered loops.

Molecular Basis for Endocrine Disruption by Pesticides Targeting Aromatase and Estrogen Receptor

Molecular Lego: design of molecular assemblies of P450 enzymes for nanobiotechnology

Optimization of the bacterial cytochrome P450 BM3 system for the production of human drug metabolites

Oxygen Stability in the New [FeFe]-Hydrogenase from Clostridium beijerinckii SM10 (CbA5H).

P450 versus P420: correlation between cyclic voltammetry and visible absorption spectroscopy of the immobilized heme domain of cytochrome P450 BM3.

P450-based porous silicon biosensor for arachidonic acid detection

Peroxide-driven catalysis of the heme domain of A. radioresistens cytochrome P450 116B5 for sustainable aromatic rings oxidation and drug metabolites production

Phenol hydroxylase from Acinetobacter radioresistens is a multicomponent enzyme. Purification and characterization of the reductase moiety

Protein Adsorption on Nanocrystalline TiO(2) Films: An Immobilization Strategy for Bioanalytical Devices

Protein and electrode engineering for the covalent immobilization of P450 BMP on gold.

Proton-coupled electron transfer of flavodoxin immobilized on nanostructured tin dioxide electrodes: thermodynamics versus kinetics control of protein redox function

Radical intermediates in veratryl alcohol oxidation by ligninase. NMR evidence

Redox properties and crystal structures of a Desulfovibrio vulgaris flavodoxin mutant in the monomeric and homodimeric forms