List of works - Reinhard Sterner

1H nuclear-magnetic-resonance investigation of oxidized Fe4S4 ferredoxin from Thermotoga maritima. Hyperfine-shifted resonances, sequence-specific assignments and secondary structure

scientific article published on 01 May 1995

2.0 A structure of indole-3-glycerol phosphate synthase from the hyperthermophile Sulfolobus solfataricus: possible determinants of protein stability

scientific article published on 15 December 1995

A Fold-Independent Interface Residue Is Crucial for Complex Formation and Allosteric Signaling in Class I Glutamine Amidotransferases

scientific article published on 23 May 2019

A common evolutionary origin of two elementary enzyme folds

scientific article

A comprehensive analysis of the geranylgeranylglyceryl phosphate synthase enzyme family identifies novel members and reveals mechanisms of substrate specificity and quaternary structure organization

scientific article

A fast and precise approach for computational saturation mutagenesis and its experimental validation by using an artificial (βα)8-barrel protein

scientific article

A histidine gene cluster of the hyperthermophile Thermotoga maritima: sequence analysis and evolutionary significance.

scientific article published in November 1998

A novel tryptophan synthase beta-subunit from the hyperthermophile Thermotoga maritima. Quaternary structure, steady-state kinetics, and putative physiological role

scientific article

A rationally designed monomeric variant of anthranilate phosphoribosyltransferase from Sulfolobus solfataricus is as active as the dimeric wild-type enzyme but less thermostable.

scientific article published on 4 December 2007

A robust protein host for anchoring chelating ligands and organocatalysts

scientific article

A sugar isomerization reaction established on various (βα)₈-barrel scaffolds is based on substrate-assisted catalysis

scientific article

Activation of a chimeric Rpb5/RpoH subunit using library selection

scientific article

Activation of anthranilate phosphoribosyltransferase from Sulfolobus solfataricus by removal of magnesium inhibition and acceleration of product release

scientific article

An NMR-derived model for the solution structure of oxidized Thermotoga maritima 1[Fe4-S4] ferredoxin

scientific article

Analysis of allosteric communication in a multienzyme complex by ancestral sequence reconstruction

scientific article published on 23 December 2019

Ancestral Tryptophan Synthase Reveals Functional Sophistication of Primordial Enzyme Complexes

scientific article published on 8 June 2016

Ancestral protein reconstruction: techniques and applications

scientific article published on September 2015

Artificial Light Regulation of an Allosteric Bienzyme Complex by a Photosensitive Ligand

scientific article published on 29 May 2018

Biochemistry. De novo design of an enzyme

scientific article

Catalysis uncoupling in a glutamine amidotransferase bienzyme by unblocking the glutaminase active site

scientific article

Catalytic versatility, stability, and evolution of the (betaalpha)8-barrel enzyme fold

scientific article

Characterisation of a thermoalkali-stable cyclodextrin glycosyltransferase from the anaerobic thermoalkaliphilic bacterium Anaerobranca gottschalkii

scientific article published on 31 August 2004

Combining ancestral sequence reconstruction with protein design to identify an interface hotspot in a key metabolic enzyme complex

scientific article published on 9 December 2016

Computational and experimental evidence for the evolution of a (beta alpha)8-barrel protein from an ancestral quarter-barrel stabilised by disulfide bonds

article

Computational design of enzymes

scientific article

Conservation of the folding mechanism between designed primordial (βα)8-barrel proteins and their modern descendant

scientific article published on 19 July 2012

Constitutively active glutaminase variants provide insights into the activation mechanism of anthranilate synthase

scientific article published on 23 March 2012

Conversion of anthranilate synthase into isochorismate synthase: implications for the evolution of chorismate-utilizing enzymes

scientific article published on September 2015

Crystal structure at 2.0 A resolution of phosphoribosyl anthranilate isomerase from the hyperthermophile Thermotoga maritima: possible determinants of protein stability

scientific article

Dimerization determines substrate specificity of a bacterial prenyltransferase

scientific article published on 21 May 2012

Directed evolution of (βα)(8)-barrel enzymes: establishing phosphoribosylanthranilate isomerisation activity on the scaffold of the tryptophan synthase α-subunit

scientific article

Directed evolution: a powerful approach to optimising and understanding enzymes

article by Reinhard Sterner published 4 July 2011 in ChemBioChem

Divergent evolution of (betaalpha)8-barrel enzymes.

scientific article

Diversity of bacteria and archaea from two shallow marine hydrothermal vents from Vulcano Island

scientific article published on 10 May 2017

Efficient expression, purification and crystallisation of two hyperthermostable enzymes of histidine biosynthesis

scientific article published on 01 July 1999

Enhancing the stability and solubility of the glucocorticoid receptor ligand-binding domain by high-throughput library screening

scientific article

Epimerisation of chiral hydroxylactones by short-chain dehydrogenases/reductases accounts for sex pheromone evolution in Nasonia

scientific article (publication date: 5 October 2016)

Establishing catalytic activity on an artificial (βα)8-barrel protein designed from identical half-barrels

scientific article

Establishing wild-type levels of catalytic activity on natural and artificial ( )8-barrel protein scaffolds

scientific article

Evidence for the existence of elaborate enzyme complexes in the Paleoarchean era

scientific article

Evolution of multi-enzyme complexes: the case of tryptophan synthase

scientific article

Evolutionary Morphing of Tryptophan Synthase: Functional Mechanisms for the Enzymatic Channeling of Indole

scientific article published on 25 October 2018

Evolutionary diversification of protein-protein interactions by interface add-ons

scientific article published on 18 September 2017

Experimental assessment of the importance of amino acid positions identified by an entropy-based correlation analysis of multiple-sequence alignments

scientific article published on 6 July 2012

Exploiting protein symmetry to design light-controllable enzyme inhibitors

scientific article published on 25 November 2013

Ferredoxin from Thermotoga maritima

scientific article published on 01 January 2001

Ferredoxin from the hyperthermophile Thermotoga maritima is stable beyond the boiling point of water.

scientific article

Folding mechanism of an extremely thermostable (βα)(8)-barrel enzyme: a high kinetic barrier protects the protein from denaturation

scientific article published on 11 April 2012

Functional Annotation and Three-Dimensional Structure of Dr0930 from Deinococcus radiodurans , a Close Relative of Phosphotriesterase in the Amidohydrolase Superfamily † ‡

scientific article

Functional assignment of an enzyme that catalyzes the synthesis of an archaea-type ether lipid in bacteria

scientific journal article

Generation of a Stand-Alone Tryptophan Synthase α-Subunit by Mimicking an Evolutionary Blueprint

scientific article published on 28 August 2019

Hexamerization of Geranylgeranylglyceryl Phosphate Synthase Ensures Structural Integrity and Catalytic Activity at High Temperatures

scientific article published on 11 April 2018

High-resolution crystal structure of an artificial (betaalpha)(8)-barrel protein designed from identical half-barrels

scientific article

Highlight issue: protein design

scientific article published on 12 November 2016

Hyperthermostable acetyl xylan esterase

scientific journal article

Identification and Characterization of Heptaprenylglyceryl Phosphate Processing Enzymes in Bacillus subtilis

scientific article

Identification and characterization of a bacterial glycerol-1-phosphate dehydrogenase: Ni(2+)-dependent AraM from Bacillus subtilis

scientific article published on 18 June 2008

Improving thermal and detergent stability of Bacillus stearothermophilus neopullulanase by rational enzyme design

scientific article published on 12 February 2015

Interconverting the catalytic activities of (betaalpha)(8)-barrel enzymes from different metabolic pathways: sequence requirements and molecular analysis

scientific article published in April 2004

Kinetic mechanism of indole-3-glycerol phosphate synthase

scientific article published on 19 December 2012

Library Generation and Auxotrophic Selection Assays in Escherichia coli and Thermus thermophilus

scientific article published in January 2018

Library Selection with a Randomized Repertoire of (βα)8-Barrel Enzymes Results in Unexpected Induction of Gene Expression

scientific article published on 07 October 2019

Ligand-induced formation of a transient tryptophan synthase complex with αββ subunit stoichiometry

scientific article published on 6 December 2010

Long-Term Persistence of Bi-functionality Contributes to the Robustness of Microbial Life through Exaptation

scientific article published on 29 January 2016

Mapping key amino acid residues for the epimerase efficiency and stereospecificity of the sex pheromone biosynthetic short-chain dehydrogenases/reductases of Nasonia

scientific article published in Scientific Reports

Mimicking enzyme evolution by generating new (betaalpha)8-barrels from (betaalpha)4-half-barrels

scientific article

Molecular Engineering of Organophosphate Hydrolysis Activity from a Weak Promiscuous Lactonase Template

scientific article

Nested allostery of arthropodan hemocyanin (Eurypelma californicum and Homarus americanus). The role of protons

scientific article published in January 1990

Phosphoribosyl anthranilate isomerase from Thermotoga maritima is an extremely stable and active homodimer.

scientific article published on October 1996

Phosphoribosylanthranilate isomerase and indoleglycerol-phosphate synthase: tryptophan biosynthetic enzymes from Thermotoga maritima.

scientific article

Photochromic coenzyme Q derivatives: switching redox potentials with light.

scientific article published on 20 July 2017

Prediction of quaternary structure by analysis of hot spot residues in protein-protein interfaces: the case of anthranilate phosphoribosyltransferases

scientific article published on 10 June 2019

Protein design at the crossroads of biotechnology, chemistry, theory, and evolution

scientific article published on 01 January 2003

Random mutagenesis suggests that sequence errors are not a major cause of variation in the activity of individual molecules of β-galactosidase

scientific article

Regulation of the hetero-octameric ATP phosphoribosyl transferase complex from Thermotoga maritima by a tRNA synthetase-like subunit.

scientific article published in February 2005

Related (βα)8-barrel proteins in histidine and tryptophan biosynthesis: a paradigm to study enzyme evolution

scientific article published on 08 June 2011

Relationship of Catalysis and Active Site Loop Dynamics in the (βα)8-Barrel Enzyme Indole-3-glycerol Phosphate Synthase

scientific article published on 08 March 2018

Role of the N-terminal extension of the (betaalpha)8-barrel enzyme indole-3-glycerol phosphate synthase for its fold, stability, and catalytic activity

scientific article

Rosetta:MSF: a modular framework for multi-state computational protein design

scientific article

Significance of the Protein Interface Configuration for Allostery in Imidazole Glycerol Phosphate Synthase

scientific article published on 13 July 2020

Small structural changes account for the high thermostability of 1[4Fe-4S] ferredoxin from the hyperthermophilic bacterium Thermotoga maritima

scientific article

Small-angle X-ray scattering reveals differences between the quaternary structures of oxygenated and deoxygenated tarantula hemocyanin

scientific article published on 01 September 1996

Stabilisation of a (betaalpha)8-barrel protein designed from identical half barrels

scientific article

Stability, catalytic versatility and evolution of the (beta alpha)(8)-barrel fold.

scientific article

Stabilization of a metabolic enzyme by library selection in Thermus thermophilus

scientific article

Standardized cloning vectors for protein production and generation of large gene libraries in Escherichia coli

scientific article published in January 2018

Structural and Mutational Analysis of Substrate Complexation by Anthranilate Phosphoribosyltransferase fromSulfolobus solfataricus

scientific article published in Journal of Biological Chemistry

Structural evidence for ammonia tunneling across the (beta alpha)(8) barrel of the imidazole glycerol phosphate synthase bienzyme complex

scientific article

Structural evidence for evolution of the beta/alpha barrel scaffold by gene duplication and fusion

scientific article

Structural features correlated with the extreme thermostability of 1[4Fe-4S] ferredoxin from the hyperthermophilic bacterium Thermotoga maritima

scientific article published on 01 March 1997

Structure and function of mutationally generated monomers of dimeric phosphoribosylanthranilate isomerase from Thermotoga maritima

scientific article

The interaction of ammonia and xenon with the imidazole glycerol phosphate synthase from Thermotoga maritima as detected by NMR spectroscopy

scientific article

TransCent: computational enzyme design by transferring active sites and considering constraints relevant for catalysis

scientific article

Tris: an allosteric effector of tarantula haemocyanin

scientific article published on 01 February 1994

TrpB2 enzymes are O-phospho-l-serine dependent tryptophan synthases

scientific article

Two (betaalpha)(8)-barrel enzymes of histidine and tryptophan biosynthesis have similar reaction mechanisms and common strategies for protecting their labile substrates

scientific article

[Hierarchies in the structure and function of oxygen-binding proteins]

scientific article published on 01 December 1990

List of works by Reinhard Sterner

1H nuclear-magnetic-resonance investigation of oxidized Fe4S4 ferredoxin from Thermotoga maritima. Hyperfine-shifted resonances, sequence-specific assignments and secondary structure

2.0 A structure of indole-3-glycerol phosphate synthase from the hyperthermophile Sulfolobus solfataricus: possible determinants of protein stability

A Fold-Independent Interface Residue Is Crucial for Complex Formation and Allosteric Signaling in Class I Glutamine Amidotransferases

A common evolutionary origin of two elementary enzyme folds

A comprehensive analysis of the geranylgeranylglyceryl phosphate synthase enzyme family identifies novel members and reveals mechanisms of substrate specificity and quaternary structure organization

A fast and precise approach for computational saturation mutagenesis and its experimental validation by using an artificial (βα)8-barrel protein

A histidine gene cluster of the hyperthermophile Thermotoga maritima: sequence analysis and evolutionary significance.

A novel tryptophan synthase beta-subunit from the hyperthermophile Thermotoga maritima. Quaternary structure, steady-state kinetics, and putative physiological role

A rationally designed monomeric variant of anthranilate phosphoribosyltransferase from Sulfolobus solfataricus is as active as the dimeric wild-type enzyme but less thermostable.

A robust protein host for anchoring chelating ligands and organocatalysts

A sugar isomerization reaction established on various (βα)₈-barrel scaffolds is based on substrate-assisted catalysis

Activation of a chimeric Rpb5/RpoH subunit using library selection

Activation of anthranilate phosphoribosyltransferase from Sulfolobus solfataricus by removal of magnesium inhibition and acceleration of product release

An NMR-derived model for the solution structure of oxidized Thermotoga maritima 1[Fe4-S4] ferredoxin

Analysis of allosteric communication in a multienzyme complex by ancestral sequence reconstruction

Ancestral Tryptophan Synthase Reveals Functional Sophistication of Primordial Enzyme Complexes

Ancestral protein reconstruction: techniques and applications

Artificial Light Regulation of an Allosteric Bienzyme Complex by a Photosensitive Ligand

Biochemistry. De novo design of an enzyme

Catalysis uncoupling in a glutamine amidotransferase bienzyme by unblocking the glutaminase active site

Catalytic versatility, stability, and evolution of the (betaalpha)8-barrel enzyme fold

Characterisation of a thermoalkali-stable cyclodextrin glycosyltransferase from the anaerobic thermoalkaliphilic bacterium Anaerobranca gottschalkii

Combining ancestral sequence reconstruction with protein design to identify an interface hotspot in a key metabolic enzyme complex

Computational and experimental evidence for the evolution of a (beta alpha)8-barrel protein from an ancestral quarter-barrel stabilised by disulfide bonds

Computational design of enzymes

Conservation of the folding mechanism between designed primordial (βα)8-barrel proteins and their modern descendant

Constitutively active glutaminase variants provide insights into the activation mechanism of anthranilate synthase

Conversion of anthranilate synthase into isochorismate synthase: implications for the evolution of chorismate-utilizing enzymes

Crystal structure at 2.0 A resolution of phosphoribosyl anthranilate isomerase from the hyperthermophile Thermotoga maritima: possible determinants of protein stability

Dimerization determines substrate specificity of a bacterial prenyltransferase

Directed evolution of (βα)(8)-barrel enzymes: establishing phosphoribosylanthranilate isomerisation activity on the scaffold of the tryptophan synthase α-subunit

Directed evolution: a powerful approach to optimising and understanding enzymes

Divergent evolution of (betaalpha)8-barrel enzymes.

Diversity of bacteria and archaea from two shallow marine hydrothermal vents from Vulcano Island

Efficient expression, purification and crystallisation of two hyperthermostable enzymes of histidine biosynthesis

Enhancing the stability and solubility of the glucocorticoid receptor ligand-binding domain by high-throughput library screening

Epimerisation of chiral hydroxylactones by short-chain dehydrogenases/reductases accounts for sex pheromone evolution in Nasonia

Establishing catalytic activity on an artificial (βα)8-barrel protein designed from identical half-barrels

Establishing wild-type levels of catalytic activity on natural and artificial ( )8-barrel protein scaffolds

Evidence for the existence of elaborate enzyme complexes in the Paleoarchean era

Evolution of multi-enzyme complexes: the case of tryptophan synthase

Evolutionary Morphing of Tryptophan Synthase: Functional Mechanisms for the Enzymatic Channeling of Indole

Evolutionary diversification of protein-protein interactions by interface add-ons

Experimental assessment of the importance of amino acid positions identified by an entropy-based correlation analysis of multiple-sequence alignments

Exploiting protein symmetry to design light-controllable enzyme inhibitors

Ferredoxin from Thermotoga maritima

Ferredoxin from the hyperthermophile Thermotoga maritima is stable beyond the boiling point of water.

Folding mechanism of an extremely thermostable (βα)(8)-barrel enzyme: a high kinetic barrier protects the protein from denaturation

Functional Annotation and Three-Dimensional Structure of Dr0930 from Deinococcus radiodurans , a Close Relative of Phosphotriesterase in the Amidohydrolase Superfamily † ‡

Functional assignment of an enzyme that catalyzes the synthesis of an archaea-type ether lipid in bacteria

Generation of a Stand-Alone Tryptophan Synthase α-Subunit by Mimicking an Evolutionary Blueprint

Hexamerization of Geranylgeranylglyceryl Phosphate Synthase Ensures Structural Integrity and Catalytic Activity at High Temperatures

High-resolution crystal structure of an artificial (betaalpha)(8)-barrel protein designed from identical half-barrels

Highlight issue: protein design

Hyperthermostable acetyl xylan esterase

Identification and Characterization of Heptaprenylglyceryl Phosphate Processing Enzymes in Bacillus subtilis

Identification and characterization of a bacterial glycerol-1-phosphate dehydrogenase: Ni(2+)-dependent AraM from Bacillus subtilis

Improving thermal and detergent stability of Bacillus stearothermophilus neopullulanase by rational enzyme design

Interconverting the catalytic activities of (betaalpha)(8)-barrel enzymes from different metabolic pathways: sequence requirements and molecular analysis

Kinetic mechanism of indole-3-glycerol phosphate synthase

Library Generation and Auxotrophic Selection Assays in Escherichia coli and Thermus thermophilus

Library Selection with a Randomized Repertoire of (βα)8-Barrel Enzymes Results in Unexpected Induction of Gene Expression

Ligand-induced formation of a transient tryptophan synthase complex with αββ subunit stoichiometry

Long-Term Persistence of Bi-functionality Contributes to the Robustness of Microbial Life through Exaptation

Mapping key amino acid residues for the epimerase efficiency and stereospecificity of the sex pheromone biosynthetic short-chain dehydrogenases/reductases of Nasonia

Mimicking enzyme evolution by generating new (betaalpha)8-barrels from (betaalpha)4-half-barrels

Molecular Engineering of Organophosphate Hydrolysis Activity from a Weak Promiscuous Lactonase Template

Nested allostery of arthropodan hemocyanin (Eurypelma californicum and Homarus americanus). The role of protons

Phosphoribosyl anthranilate isomerase from Thermotoga maritima is an extremely stable and active homodimer.

Phosphoribosylanthranilate isomerase and indoleglycerol-phosphate synthase: tryptophan biosynthetic enzymes from Thermotoga maritima.

Photochromic coenzyme Q derivatives: switching redox potentials with light.

Prediction of quaternary structure by analysis of hot spot residues in protein-protein interfaces: the case of anthranilate phosphoribosyltransferases

Protein design at the crossroads of biotechnology, chemistry, theory, and evolution

Random mutagenesis suggests that sequence errors are not a major cause of variation in the activity of individual molecules of β-galactosidase

Regulation of the hetero-octameric ATP phosphoribosyl transferase complex from Thermotoga maritima by a tRNA synthetase-like subunit.

Related (βα)8-barrel proteins in histidine and tryptophan biosynthesis: a paradigm to study enzyme evolution

Relationship of Catalysis and Active Site Loop Dynamics in the (βα)8-Barrel Enzyme Indole-3-glycerol Phosphate Synthase

Role of the N-terminal extension of the (betaalpha)8-barrel enzyme indole-3-glycerol phosphate synthase for its fold, stability, and catalytic activity

Rosetta:MSF: a modular framework for multi-state computational protein design