List of works - Kalle Saksela

Harnessing endogenous miRNAs to control virus tissue tropism as a strategy for developing attenuated virus vaccines

scientific article published on September 2008

High-throughput phosphotyrosine profiling using SH2 domains

scientific article

SH3 domain ligand binding: What's the consensus and where's the specificity?

scientific article

Identification of preferred protein interactions by phage-display of the human Src homology-3 proteome

scientific article

SH3 domain-mediated recruitment of host cell amphiphysins by alphavirus nsP3 promotes viral RNA replication

scientific article published on 17 November 2011

Generation of a conditionally replicating adenovirus based on targeted destruction of E1A mRNA by a cell type-specific MicroRNA.

scientific article

Insulin receptor tyrosine kinase substrate links the E. coli O157:H7 actin assembly effectors Tir and EspF(U) during pedestal formation

scientific article

Avian and 1918 Spanish influenza a virus NS1 proteins bind to Crk/CrkL Src homology 3 domains to activate host cell signaling

scientific article

Reciprocal regulation of SH3 and SH2 domain binding via tyrosine phosphorylation of a common site in CD3epsilon

scientific article

HIV Nef, paxillin, and Pak1/2 regulate activation and secretion of TACE/ADAM10 proteases

scientific article

Dystroglycan, Tks5 and Src mediated assembly of podosomes in myoblasts

scientific article

A crucial role in cell spreading for the interaction of Abl PxxP motifs with Crk and Nck adaptors

scientific article

Human immunodeficiency virus type 1 Nef recruits the guanine exchange factor Vav1 via an unexpected interface into plasma membrane microdomains for association with p21-activated kinase 2 activity

scientific article

Nef proteins from simian immunodeficiency virus-infected chimpanzees interact with p21-activated kinase 2 and modulate cell surface expression of various human receptors

scientific article

Modulation of cell spreading and cell-substrate adhesion dynamics by dystroglycan.

scientific article

Molecular priming of Lyn by GPVI enables an immune receptor to adopt a hemostatic role

scientific article

Nef associates with p21-activated kinase 2 in a p21-GTPase-dependent dynamic activation complex within lipid rafts.

scientific article published on December 2004

Recognition of tandem PxxP motifs as a unique Src homology 3-binding mode triggers pathogen-driven actin assembly

scientific article

Further studies on hepatitis C virus NS5A-SH3 domain interactions: identification of residues critical for binding and implications for viral RNA replication and modulation of cell signalling

scientific article

HIV-Nef and ADAM17-Containing Plasma Extracellular Vesicles Induce and Correlate with Immune Pathogenesis in Chronic HIV Infection

scientific article published on 3 March 2016

Autoimmune regulator induced changes in the gene expression profile of human monocyte-dendritic cell-lineage

scientific article

Attenuation of Semliki Forest virus neurovirulence by microRNA-mediated detargeting

scientific article published on 17 October 2012

Unmodified histone H3K4 and DNA-dependent protein kinase recruit autoimmune regulator to target genes

scientific article published on 6 February 2012

Cdc42/Rac1-mediated activation primes PAK2 for superactivation by tyrosine phosphorylation

scientific article

Hepatitis C virus NS5A inhibits mixed lineage kinase 3 to block apoptosis

scientific article

Role of the Kaposi's sarcoma-associated herpesvirus K15 SH3 binding site in inflammatory signaling and B-cell activation

scientific article

Conformation of the dileucine-based sorting motif in HIV-1 Nef revealed by intermolecular domain assembly

scientific article

The mechanism of tissue-restricted antigen gene expression by AIRE.

scientific article published on March 2013

Association of Nef with p21-activated kinase 2 is dispensable for efficient human immunodeficiency virus type 1 replication and cytopathicity in ex vivo-infected human lymphoid tissue

scientific article

Interactions of the HIV/SIV Pathogenicity Factor Nef with SH3 Domain-Containing Host Cell Proteins

scientific article published on October 1, 2011

MicroRNA-mediated suppression of oncolytic adenovirus replication in human liver

scientific article

Patient mutation in AIRE disrupts P-TEFb binding and target gene transcription

scientific article

Enterohaemorrhagic Escherichia Coli Exploits a Tryptophan Switch to Hijack Host F-Actin Assembly

scientific article

Structural basis of PxxDY motif recognition in SH3 binding

scientific article

Single-domain antibody-SH3 fusions for efficient neutralization of HIV-1 Nef functions

scientific article published on 15 February 2012

Association with PAK2 Enables Functional Interactions of Lentiviral Nef Proteins with the Exocyst Complex

scientific article

Regulation of T cell activation by HIV-1 accessory proteins: Vpr acts via distinct mechanisms to cooperate with Nef in NFAT-directed gene expression and to promote transactivation by CREB

scientific article (publication date: 25 May 2003)

Identification of a highly conserved valine-glycine-phenylalanine amino acid triplet required for HIV-1 Nef function

scientific article published on 27 April 2012

Activation of p21-activated kinase 2 and its association with Nef are conserved in murine cells but are not sufficient to induce an AIDS-like disease in CD4C/HIV transgenic mice

scientific article

Alternative splicing of ADAM15 regulates its interactions with cellular SH3 proteins

scientific article

Macrophage metalloelastase (MME) as adjuvant for intra-tumoral injection of oncolytic adenovirus and its influence on metastases development

article

Optimized mouse model for the imaging of tumor metastasis upon experimental therapy

scientific article

MicroRNA-Attenuated Clone of Virulent Semliki Forest Virus Overcomes Antiviral Type I Interferon in Resistant Mouse CT-2A Glioma

scientific article

Structural Basis of the High Affinity Interaction between the Alphavirus Nonstructural Protein-3 (nsP3) and the SH3 Domain of Amphiphysin-2

scientific article published on 6 June 2016

Structural basis for the inhibition of HIV-1 Nef by a high-affinity binding single-domain antibody

scientific article

High-affinity target binding engineered via fusion of a single-domain antibody fragment with a ligand-tailored SH3 domain

scientific article

Therapeutic targeting of interactions between Nef and host cell proteins

scientific article

Hepatitis C virus NS5A protein blocks epidermal growth factor receptor degradation via a proline motif- dependent interaction

scientific article published on 13 April 2015

Versatile retargeting of SH3 domain binding by modification of non-conserved loop residues

scientific article

Comprehensive analysis of interactions between the Src-associated protein in mitosis of 68 kDa and the human Src-homology 3 proteome

scientific article published in 2012

List of works by Kalle Saksela

Harnessing endogenous miRNAs to control virus tissue tropism as a strategy for developing attenuated virus vaccines

High-throughput phosphotyrosine profiling using SH2 domains

SH3 domain ligand binding: What's the consensus and where's the specificity?

Identification of preferred protein interactions by phage-display of the human Src homology-3 proteome

SH3 domain-mediated recruitment of host cell amphiphysins by alphavirus nsP3 promotes viral RNA replication

Generation of a conditionally replicating adenovirus based on targeted destruction of E1A mRNA by a cell type-specific MicroRNA.

Insulin receptor tyrosine kinase substrate links the E. coli O157:H7 actin assembly effectors Tir and EspF(U) during pedestal formation

Avian and 1918 Spanish influenza a virus NS1 proteins bind to Crk/CrkL Src homology 3 domains to activate host cell signaling

Reciprocal regulation of SH3 and SH2 domain binding via tyrosine phosphorylation of a common site in CD3epsilon

HIV Nef, paxillin, and Pak1/2 regulate activation and secretion of TACE/ADAM10 proteases

Dystroglycan, Tks5 and Src mediated assembly of podosomes in myoblasts

A crucial role in cell spreading for the interaction of Abl PxxP motifs with Crk and Nck adaptors

Human immunodeficiency virus type 1 Nef recruits the guanine exchange factor Vav1 via an unexpected interface into plasma membrane microdomains for association with p21-activated kinase 2 activity

Nef proteins from simian immunodeficiency virus-infected chimpanzees interact with p21-activated kinase 2 and modulate cell surface expression of various human receptors

Modulation of cell spreading and cell-substrate adhesion dynamics by dystroglycan.

Molecular priming of Lyn by GPVI enables an immune receptor to adopt a hemostatic role

Nef associates with p21-activated kinase 2 in a p21-GTPase-dependent dynamic activation complex within lipid rafts.

Recognition of tandem PxxP motifs as a unique Src homology 3-binding mode triggers pathogen-driven actin assembly

Further studies on hepatitis C virus NS5A-SH3 domain interactions: identification of residues critical for binding and implications for viral RNA replication and modulation of cell signalling

HIV-Nef and ADAM17-Containing Plasma Extracellular Vesicles Induce and Correlate with Immune Pathogenesis in Chronic HIV Infection

Autoimmune regulator induced changes in the gene expression profile of human monocyte-dendritic cell-lineage

Attenuation of Semliki Forest virus neurovirulence by microRNA-mediated detargeting

Unmodified histone H3K4 and DNA-dependent protein kinase recruit autoimmune regulator to target genes

Cdc42/Rac1-mediated activation primes PAK2 for superactivation by tyrosine phosphorylation

Hepatitis C virus NS5A inhibits mixed lineage kinase 3 to block apoptosis

Role of the Kaposi's sarcoma-associated herpesvirus K15 SH3 binding site in inflammatory signaling and B-cell activation

Conformation of the dileucine-based sorting motif in HIV-1 Nef revealed by intermolecular domain assembly

The mechanism of tissue-restricted antigen gene expression by AIRE.

Association of Nef with p21-activated kinase 2 is dispensable for efficient human immunodeficiency virus type 1 replication and cytopathicity in ex vivo-infected human lymphoid tissue

Interactions of the HIV/SIV Pathogenicity Factor Nef with SH3 Domain-Containing Host Cell Proteins

MicroRNA-mediated suppression of oncolytic adenovirus replication in human liver

Patient mutation in AIRE disrupts P-TEFb binding and target gene transcription

Enterohaemorrhagic Escherichia Coli Exploits a Tryptophan Switch to Hijack Host F-Actin Assembly

Structural basis of PxxDY motif recognition in SH3 binding

Single-domain antibody-SH3 fusions for efficient neutralization of HIV-1 Nef functions

Association with PAK2 Enables Functional Interactions of Lentiviral Nef Proteins with the Exocyst Complex

Regulation of T cell activation by HIV-1 accessory proteins: Vpr acts via distinct mechanisms to cooperate with Nef in NFAT-directed gene expression and to promote transactivation by CREB

Identification of a highly conserved valine-glycine-phenylalanine amino acid triplet required for HIV-1 Nef function

Activation of p21-activated kinase 2 and its association with Nef are conserved in murine cells but are not sufficient to induce an AIDS-like disease in CD4C/HIV transgenic mice

Alternative splicing of ADAM15 regulates its interactions with cellular SH3 proteins

Macrophage metalloelastase (MME) as adjuvant for intra-tumoral injection of oncolytic adenovirus and its influence on metastases development

Optimized mouse model for the imaging of tumor metastasis upon experimental therapy

MicroRNA-Attenuated Clone of Virulent Semliki Forest Virus Overcomes Antiviral Type I Interferon in Resistant Mouse CT-2A Glioma

Structural Basis of the High Affinity Interaction between the Alphavirus Nonstructural Protein-3 (nsP3) and the SH3 Domain of Amphiphysin-2

Structural basis for the inhibition of HIV-1 Nef by a high-affinity binding single-domain antibody

High-affinity target binding engineered via fusion of a single-domain antibody fragment with a ligand-tailored SH3 domain

Therapeutic targeting of interactions between Nef and host cell proteins

Hepatitis C virus NS5A protein blocks epidermal growth factor receptor degradation via a proline motif- dependent interaction

Versatile retargeting of SH3 domain binding by modification of non-conserved loop residues

Comprehensive analysis of interactions between the Src-associated protein in mitosis of 68 kDa and the human Src-homology 3 proteome