List of works by Ronald Melki

2D polarization imaging as a low-cost fluorescence method to detect α-synuclein aggregation ex vivo in models of Parkinson's disease

scientific article published on 02 October 2018

<i>C9ORF72</i>-derived poly-GA DPRs undergo endocytic uptake in iAstrocytes and spread to motor neurons

scientific article published on 13 May 2022

A mutation within the C-terminal domain of Sup35p that affects [PSI+] prion propagation

scientific article published on 16 June 2011

A native-like conformation for the C-terminal domain of the prion Ure2p within its fibrillar form

scientific article published on 03 July 2012

A new amyloidosis caused by fibrillar aggregates of mutated corneodesmosin.

scientific article published on 6 May 2010

A novel bio-orthogonal cross-linker for improved protein/protein interaction analysis

scientific article published on 12 January 2015

A probabilistic framework for peptide and protein quantification from data-dependent and data-independent LC-MS proteomics experiments

scientific article

A prolonged chronological lifespan is an unexpected benefit of the [PSI+] prion in yeast

scientific article published on 14 September 2017

A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry

scientific article

A role for the proteasome in the turnover of Sup35p and in [PSI(+) ] prion propagation.

scientific article published on 17 March 2014

Alpha-synuclein spreading in M83 mice brain revealed by detection of pathological α-synuclein by enhanced ELISA.

scientific article

Alpha-synuclein transfers from neurons to oligodendrocytes

scientific article

An Efficient Procedure for Removal and Inactivation of Alpha-Synuclein Assemblies from Laboratory Materials

scientific article

Assembly of the asparagine- and glutamine-rich yeast prions into protein fibrils

scientific article published on June 2008

Assembly of the yeast prion Ure2p into protein fibrils. Thermodynamic and kinetic characterization

scientific article published on 30 May 2003

Assessment of the efficacy of different procedures that remove and disassemble alpha-synuclein, tau and A-beta fibrils from laboratory material and surfaces.

scientific article published on 17 July 2018

Atomic force microscopy imaging and nanomechanical properties of six Tau isoforms assemblies

scientific article published on 17 November 2020

Axonal transport and secretion of fibrillar forms of α-synuclein, Aβ42 peptide and HTTExon 1

scientific article published on 28 January 2016

Biochemical and functional analysis of the assembly of full-length Sup35p and its prion-forming domain

scientific article published on 22 November 2006

CONFINE-MAS: a magic-angle spinning NMR probe that confines the sample in case of a rotor explosion

scientific article published on 10 December 2018

Cell biology and dynamics of Neuronal Na+/K+-ATPase in health and diseases

scientific article published on 11 December 2018

Cellular response of human neuroblastoma cells to α-synuclein fibrils, the main constituent of Lewy bodies

scientific article published on 12 October 2015

Characterization of monomeric intermediates during VSV glycoprotein structural transition

scientific article

Characterization of the cytoplasmic chaperonin containing TCP-1 from the Antarctic fish Notothenia coriiceps

scientific article published on 13 June 2006

Clustering of Tau fibrils impairs the synaptic composition of α3-Na/K-ATPase and AMPA receptors

article

Crucial Role of FABP3 in αSyn-Induced Reduction of Septal GABAergic Neurons and Cognitive Decline in Mice

scientific article published on 01 January 2021

Cyan fluorescent protein carries a constitutive mutation that prevents its dimerization

scientific article published on 30 December 2010

Cytoplasmic penetration and persistent infection of mammalian cells by polyglutamine aggregates

scientific article

DNAJB6 is a peptide-binding chaperone which can suppress amyloid fibrillation of polyglutamine peptides at substoichiometric molar ratios.

scientific article published on August 2013

Data in support of the identification of neuronal and astrocyte proteins interacting with extracellularly applied oligomeric and fibrillar α-synuclein assemblies by mass spectrometry

scientific article

Delaying aging and the aging-associated decline in protein homeostasis by inhibition of tryptophan degradation.

scientific article

Demonstration of prion-like properties of mutant huntingtin fibrils in both in vitro and in vivo paradigms

scientific article published on 20 February 2019

Detection of alpha-synuclein aggregates in gastrointestinal biopsies by protein misfolding cyclic amplification

scientific article published on 09 May 2019

Different roles of two gamma-tubulin isotypes in the cytoskeleton of the Antarctic ciliate Euplotes focardii: remodelling of interaction surfaces may enhance microtubule nucleation at low temperature

scientific article published on November 2008

Differential Membrane Binding and Seeding of Distinct α-Synuclein Fibrillar Polymorphs

scientific article published on 28 January 2020

Dipeptide repeat derived from C9orf72 hexanucleotide expansions forms amyloids or natively unfolded structures in vitro

scientific article published on 27 March 2020

Direct evidence of Parkinson pathology spread from the gastrointestinal tract to the brain in rats

scientific article

Disassembly of Tau fibrils by the human Hsp70 disaggregation machinery generates small seeding-competent species

scientific article published on 28 May 2020

Distinct alpha-Synuclein species induced by seeding are selectively cleared by the Lysosome or the Proteasome in neuronally differentiated SH-SY5Y cells

scientific article published on 31 August 2020

Dynamical properties of α-synuclein in soluble and fibrillar forms by Quasi Elastic Neutron Scattering

scientific article published on 21 April 2014

Ectosomes: a new mechanism for non-exosomal secretion of tau protein

scientific article

Effect of tolytoxin on tunneling nanotube formation and function

scientific article published on 05 April 2019

Endocytic vesicle rupture is a conserved mechanism of cellular invasion by amyloid proteins

scientific article published on 19 May 2017

Endogenous alpha-synuclein monomers, oligomers and resulting pathology: let's talk about the lipids in the room

scientific article published on 12 November 2019

Extensive de novo solid-state NMR assignments of the 33 kDa C-terminal domain of the Ure2 prion

scientific article published on 31 July 2011

Fatty Acid-Binding Protein 3 is Critical for α-Synuclein Uptake and MPP+-Induced Mitochondrial Dysfunction in Cultured Dopaminergic Neurons

scientific article published on 28 October 2019

Fibrillar structure and charge determine the interaction of polyglutamine protein aggregates with the cell surface

scientific article

Fibrillar α-synuclein and huntingtin exon 1 assemblies are toxic to the cells

scientific article

Further exploration of the conformational space of α-synuclein fibrils: solid-state NMR assignment of a high-pH polymorph

scientific article published on 30 August 2015

G51D α-synuclein mutation causes a novel parkinsonian-pyramidal syndrome

scientific article

Glucose availability dictates the export of the soluble and prion forms of Sup35p via periplasmic or extracellular vesicles

scientific article published on 27 April 2020

Growth phase-dependent changes in the size and infectivity of SDS-resistant Sup35p assemblies associated with the [PSI+ ] prion in yeast

scientific article published on 27 June 2019

Heterologous expression and folding analysis of a beta-tubulin isotype from the Antarctic ciliate Euplotes focardii.

scientific article published in December 2002

How the shapes of seeds can influence pathology

scientific article published on 28 March 2017

Hsc70 protein interaction with soluble and fibrillar alpha-synuclein

scientific article

Hydrogen/deuterium exchange mass spectrometric analysis of conformational changes accompanying the assembly of the yeast prion Ure2p into protein fibrils

scientific article published on 12 April 2007

Identification and characterization of a novel human methyltransferase modulating Hsp70 protein function through lysine methylation

scientific article

Identification of protein interfaces between α-synuclein, the principal component of Lewy bodies in Parkinson disease, and the molecular chaperones human Hsc70 and the yeast Ssa1p.

scientific article

Increased Immune Activation by Pathologic α-Synuclein in Parkinson's Disease

scientific article published on 15 August 2019

Infectious properties of protein aggregates involved in neurodegenerative diseases

scientific article published on 23 May 2013

Insights into the architecture of the Ure2p yeast protein assemblies from helical twisted fibrils

scientific article

Interaction of the chaperones alpha B-crystallin and CHIP with fibrillar alpha-synuclein: Effects on internalization by cells and identification of interacting interfaces

scientific article published on 16 May 2020

KLK6 proteolysis is implicated in the turnover and uptake of extracellular alpha-synuclein species.

scientific article

LAG3 is not expressed in human and murine neurons and does not modulate α-synucleinopathies

scientific article published on 26 July 2021

LRRK2 modifies α-syn pathology and spread in mouse models and human neurons.

scientific article published on 29 March 2019

Methyl-branched lipids promote the membrane adsorption of α-synuclein by enhancing shallow lipid-packing defects

scientific article published on 31 March 2015

Molecular and functional characterization of the only known hemiascomycete ortholog of the carboxyl terminus of Hsc70-interacting protein CHIP in the yeast Yarrowia lipolytica

scientific article published on 29 October 2011

Molecular chaperones and the assembly of the prion Sup35p, an in vitro study

scientific article

Molecular chaperones and the assembly of the prion Ure2p in vitro

scientific article published on 8 April 2008

Molecular interaction between the chaperone Hsc70 and the N-terminal flank of huntingtin exon 1 modulates aggregation

scientific article

More than just trash bins? Potential roles for extracellular vesicles in the vertical and horizontal transmission of yeast prions

scientific article

Nanomechanical properties of distinct fibrillar polymorphs of the protein α-synuclein

scientific article

Neuron-to-neuron transmission of α-synuclein fibrils through axonal transport

scientific article

Novel antibodies detect additional α-synuclein pathology in synucleinopathies: potential development for immunotherapy

scientific article published on 30 November 2020

Oligomerization of the SPP1 scaffolding protein

scientific article published on 23 February 2008

Overexpression of the calpain-specific inhibitor calpastatin reduces human alpha-Synuclein processing, aggregation and synaptic impairment in [A30P]αSyn transgenic mice

scientific article

PA700, the regulatory complex of the 26S proteasome, interferes with alpha-synuclein assembly

scientific article published in August 2005

Packing of the prion Ure2p in protein fibrils probed by fluorescence X-ray near-edge structure spectroscopy at sulfur K-edge

scientific article published on 20 December 2005

Peripheral Inflammation Regulates CNS Immune Surveillance Through the Recruitment of Inflammatory Monocytes Upon Systemic α-Synuclein Administration

PhLP3 modulates CCT-mediated actin and tubulin folding via ternary complexes with substrates

scientific article

Physico-Pathologic Mechanisms Involved in Neurodegeneration: Misfolded Protein-Plasma Membrane Interactions

scientific article

Polyglutamine repeats are associated to specific sequence biases that are conserved among eukaryotes.

scientific article

Polypeptides derived from α-Synuclein binding partners to prevent α-Synuclein fibrils interaction with and take-up by cells

scientific article published on 13 August 2020

Prion Amyloid Polymorphs - The Tag Might Change It All

scientific article published on 06 August 2020

Prion fibrils of Ure2p assembled under physiological conditions contain highly ordered, natively folded modules

scientific article published on 11 September 2009

Prion-like transmission of protein aggregates in neurodegenerative diseases

scientific article

Propagation of α-Synuclein Strains within Human Reconstructed Neuronal Network

scientific article published on 10 January 2019

Protein Quality Control Pathways at the Crossroad of Synucleinopathies

scientific article published on 01 January 2020

Protein misfolding and aggregation in ageing and disease: molecular processes and therapeutic perspectives

scientific article published on 29 April 2007

Prying into the Prion Hypothesis for Parkinson's Disease

scientific article

Qualitative and quantitative multiplexed proteomic analysis of complex yeast protein fractions that modulate the assembly of the yeast prion Sup35p

scientific article (publication date: 2011)

Recommendations of the Global Multiple System Atrophy Research Roadmap Meeting.

scientific article published on 13 December 2017

Similar and divergent features in mammalian and yeast prions

scientific article

Simultaneous measurement of amyloid fibril formation by dynamic light scattering and fluorescence reveals complex aggregation kinetics

scientific article

Solid-state NMR sequential assignments of the amyloid core of Sup35pNM.

scientific article

Solid-state NMR sequential assignments of the amyloid core of full-length Sup35p

scientific article

Solid-state NMR sequential assignments of α-synuclein

scientific article

Spreading of a prion domain from cell-to-cell by vesicular transport in Caenorhabditis elegans

scientific article

Structural and functional characterization of two alpha-synuclein strains

scientific article published on January 2013

Structural and functional properties of prefibrillar α-synuclein oligomers

scientific article published on 14 April 2016

Structural characterization of the fibrillar form of the yeast Saccharomyces cerevisiae prion Ure2p

scientific article

Structure and assembly properties of the N-terminal domain of the prion Ure2p in isolation and in its natural context

scientific article

Structure and assembly properties of the yeast prion Ure2p

scientific article published in January 2002

Structure of the prion Ure2p in protein fibrils assembled in vitro

scientific article

Sup35p in Its Soluble and Prion States Is Packaged inside Extracellular Vesicles

scientific article

Swa2p-dependent clathrin dynamics is critical for Flo11p processing and 'Mat' formation in the yeast Saccharomyces cerevisiae

scientific article published on 09 February 2010

Synthetic lipid vesicles recruit native-like aggregates and affect the aggregation process of the prion Ure2p: insights on vesicle permeabilization and charge selectivity.

scientific article published on April 2009

The 26S Proteasome Degrades the Soluble but Not the Fibrillar Form of the Yeast Prion Ure2p In Vitro

scientific article published on 26 June 2015

The Amphipathic GM1 Molecule Stabilizes Amyloid Aggregates, Preventing their Cytotoxicity

scientific article published on 12 June 2020

The N terminus of the small heat shock protein HSPB7 drives its polyQ aggregation-suppressing activity

scientific article published on 16 May 2019

The S/T-Rich Motif in the DNAJB6 Chaperone Delays Polyglutamine Aggregation and the Onset of Disease in a Mouse Model.

scientific article published on 12 April 2016

The Yarrowia lipolytica orthologs of Sup35p assemble into thioflavin T-negative amyloid fibrils

scientific article published on 14 July 2020

The conformation of the prion domain of Sup35p in isolation and in the full-length protein

scientific article published on 9 October 2013

The cytosolic chaperonin CCT associates to cytoplasmic microtubular structures during mammalian spermiogenesis and to heterochromatin in germline and somatic cells

scientific journal article

The cytosolic chaperonin CCT/TRiC and cancer cell proliferation

scientific article

The ectodomain of the viral receptor YueB forms a fiber that triggers ejection of bacteriophage SPP1 DNA

scientific article published on 14 February 2006

The expression level of alpha-synuclein in different neuronal populations is the primary determinant of its prion-like seeding

scientific article published on 17 March 2020

The interaction of Hsc70 protein with fibrillar α-Synuclein and its therapeutic potential in Parkinson's disease

scientific article published on January 2012

The native-like conformation of Ure2p in fibrils assembled under physiologically relevant conditions switches to an amyloid-like conformation upon heat-treatment of the fibrils

scientific article

The structural differences between patient-derived α-synuclein strains dictate characteristics of Parkinson's disease, multiple system atrophy and dementia with Lewy bodies

scientific article published on 30 April 2020

The yeast prion Ure2p native-like assemblies are toxic to mammalian cells regardless of their aggregation state.

scientific article published on 29 March 2006

The yeast prion Ure2p retains its native alpha-helical conformation upon assembly into protein fibrils in vitro

scientific article

Toxic effects of amyloid fibrils on cell membranes: the importance of ganglioside GM1.

scientific article

Transfer of human α-synuclein from the olfactory bulb to interconnected brain regions in mice

scientific article

Tubulin folding: the special case of a beta-tubulin isotype from the Antarctic psychrophilic ciliate Euplotes focardii

Tunneling nanotube (TNT)-mediated neuron-to neuron transfer of pathological Tau protein assemblies

scientific article

Tunneling nanotubes spread fibrillar α-synuclein by intercellular trafficking of lysosomes

scientific article published on 22 August 2016

Two new polymorphic structures of human full-length alpha-synuclein fibrils solved by cryo-electron microscopy

scientific article published on 09 December 2019

Unlike twins: an NMR comparison of two α-synuclein polymorphs featuring different toxicity

scientific article (publication date: 2014)

Wheat-germ cell-free production of prion proteins for solid-state NMR structural studies

scientific article published on 04 July 2010

Yeast prions assembly and propagation: contributions of the prion and non-prion moieties and the nature of assemblies

scientific article

Yet another polymorph of α-synuclein: solid-state sequential assignments

scientific article published on 10 October 2013

[Prion proteins: folding and aggregation properties]

scientific article published on 01 June 2005

α-Synuclein and huntingtin exon 1 amyloid fibrils bind laterally to the cellular membrane

scientific article

α-Synuclein conformational strains spread, seed and target neuronal cells differentially after injection into the olfactory bulb

scientific article published on 30 December 2019

α-Synuclein propagates from mouse brain to grafted dopaminergic neurons and seeds aggregation in cultured human cells

scientific article

α-Synuclein transfer between neurons and astrocytes indicates that astrocytes play a role in degradation rather than in spreading

scientific article published on 19 July 2017

α-synuclein assemblies sequester neuronal α3-Na+/K+-ATPase and impair Na+ gradient

scientific article

α-synuclein oligomers and fibrils: a spectrum of species, a spectrum of toxicities

scientific article published on 02 August 2019

β-amyloid and ATP-induced diffusional trapping of astrocyte and neuronal metabotropic glutamate type-5 receptors.

scientific article published on 6 August 2013

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