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List of works by Robert Hausinger

A covalent protein-DNA 5'-product adduct is generated following AP lyase activity of human ALKBH1 (AlkB homologue 1)

scientific article

ALKBH1 is dispensable for abasic site cleavage during base excision repair and class switch recombination

scientific article

ALKBH7 Variant Related to Prostate Cancer Exhibits Altered Substrate Binding

scientific article

Aberrant activity of the DNA repair enzyme AlkB.

scientific article

Alcaligenes eutrophus JMP134 "2,4-dichlorophenoxyacetate monooxygenase" is an alpha-ketoglutarate-dependent dioxygenase

scientific article published on April 1993

Alternative reactivity of an alpha-ketoglutarate-dependent iron(II) oxygenase: enzyme self-hydroxylation

scientific article published in May 2001

Amino acid sequence of a 3Fe:3S ferredoxin from the "archaebacterium" Methanosarcina barkeri (DSM 800)

scientific article published in Journal of Biological Chemistry

An assay for Fe(II)/2-oxoglutarate-dependent dioxygenases by enzyme-coupled detection of succinate formation.

scientific article

Analysis of a soluble (UreD:UreF:UreG)2 accessory protein complex and its interactions with Klebsiella aerogenes urease by mass spectrometry

scientific article

Analysis of the Active Site Cysteine Residue of the Sacrificial Sulfur Insertase LarE from Lactobacillus plantarum

scientific article published on 11 September 2018

Apoprotein isolation and activation, and vibrational structure of the Helicobacter mustelae iron urease

scientific article published on 28 November 2011

Ascorbic Acid-Dependent Turnover and Reactivation of 2,4-Dichlorophenoxyacetic Acid/α-Ketoglutarate Dioxygenase Using Thiophenoxyacetic Acid†

article

Assays for Allantoinase

article

Biochemical Characterization of AP Lyase and m6A Demethylase Activities of Human AlkB Homologue 1 (ALKBH1).

scientific article published on 14 March 2017

Biosynthesis of active Bacillus subtilis urease in the absence of known urease accessory proteins

scientific article

Biosynthesis of metal sites

scientific article published on February 2004

Biosynthesis of the nickel-pincer nucleotide cofactor of lactate racemase requires a CTP-dependent cyclometallase

scientific article published in Journal of Biological Chemistry

Biosynthesis of the urease metallocenter

scientific article published on 28 March 2013

Calorimetric assessment of Fe(2+) binding to α-ketoglutarate/taurine dioxygenase: ironing out the energetics of metal coordination by the 2-His-1-carboxylate facial triad

scientific article

Catalytic Mechanisms of Fe(II)- and 2-Oxoglutarate-dependent Oxygenases

scientific article

Characterization of UreG, identification of a UreD-UreF-UreG complex, and evidence suggesting that a nucleotide-binding site in UreG is required for in vivo metallocenter assembly of Klebsiella aerogenes urease ⬇️

scientific article published on July 1, 1997

Characterization of a Trypanosoma brucei Alkb homolog capable of repairing alkylated DNA

scientific article

Characterization of a chromosomally encoded 2,4-dichlorophenoxyacetic acid/alpha-ketoglutarate dioxygenase from Burkholderia sp. strain RASC

scientific article

Characterization of active site variants of xanthine hydroxylase from Aspergillus nidulans

scientific article

Characterization of human AlkB homolog 1 produced in mammalian cells and demonstration of mitochondrial dysfunction in ALKBH1-deficient cells

scientific article published on 30 October 2017

Characterization of metal-substituted Klebsiella aerogenes urease

scientific article

Characterization of the Klebsiella aerogenes urease accessory protein UreD in fusion with the maltose binding protein

scientific article published on 5 March 2010

Characterization of the first enzyme in 2,4-dichlorophenoxyacetic acid metabolism

scientific article published on June 1995

Characterization of the mononickel metallocenter in H134A mutant urease

scientific article

Characterization of urease from Sporosarcina ureae

scientific article published in January 1992

Chemical cross-linking and mass spectrometric identification of sites of interaction for UreD, UreF, and urease

scientific article

Chemical rescue of Klebsiella aerogenes urease variants lacking the carbamylated-lysine nickel ligand

scientific article

Cloning and characterization of a sulfonate/alpha-ketoglutarate dioxygenase from Saccharomyces cerevisiae.

scientific article

Competitive inhibitors of Klebsiella aerogenes urease. Mechanisms of interaction with the nickel active site.

scientific article published in September 1989

Correction to Biochemical and Spectroscopic Characterization of the Non-Heme Fe(II)- and 2-Oxoglutarate-Dependent Ethylene-Forming Enzyme from Pseudomonas syringae pv. phaseolicola PK2.

scientific article published on 7 June 2017

Cr(II) reactivity of taurine/alpha-ketoglutarate dioxygenase

scientific article published on November 2007

Crystal structure of a truncated urease accessory protein UreF from Helicobacter pylori

scientific article

Diethylpyrocarbonate reactivity ofKlebsiella aerogenes urease: Effect ofpH and active site ligands on the rate of inactivation

scientific article published on 01 February 1993

Direct detection of oxygen intermediates in the non-heme Fe enzyme taurine/alpha-ketoglutarate dioxygenase.

scientific article

Distribution of the tfdA Gene in Soil Bacteria That Do Not Degrade 2,4-Dichlorophenoxyacetic Acid (2,4-D)

article

Dual effects of ionic strength on Klebsiella aerogenes urease: pH-dependent activation and inhibition

scientific article

Evidence for the presence of urease apoprotein complexes containing UreD, UreF, and UreG in cells that are competent for in vivo enzyme activation.

scientific article published on April 1995

Factor 390 chromophores: phosphodiester between AMP or GMP and methanogen factor 420.

scientific article published on March 1985

Fe(II)/alpha-ketoglutarate hydroxylases involved in nucleobase, nucleoside, nucleotide, and chromatin metabolism

scientific article published on 27 June 2008

FeII/alpha-ketoglutarate-dependent hydroxylases and related enzymes

scientific article

Fluoride Inhibition ofKlebsiella aerogenesUrease: Mechanistic Implications of a Pseudo-uncompetitive, Slow-Binding Inhibitor†

article

Fructose-1,6-bisphosphate aldolase (class II) is the primary site of nickel toxicity in Escherichia coli

scientific article published on 8 November 2011

Function of UreB in Klebsiella aerogenes urease

scientific article

Genetic engineering of cyanobacteria to enhance biohydrogen production from sunlight and water

scientific article (publication date: 2012)

Herbicide-Degrading α-Keto Acid-Dependent Enzyme TfdA: Metal Coordination Environment and Mechanistic Insights†

article

Homology modeling, molecular dynamics, and site-directed mutagenesis study of AlkB human homolog 1 (ALKBH1)

scientific article

Human AlkB homologue 1 (ABH1) exhibits DNA lyase activity at abasic sites

scientific article

Identification of Escherichia coli YgaF as an L-2-hydroxyglutarate oxidase

scientific article published on 4 April 2008

Identification of the essential cysteine residue in Klebsiella aerogenes urease ⬇️

scientific article published in Journal of Biological Chemistry

In Vivo and in Vitro Kinetics of Metal Transfer by the Klebsiella aerogenes Urease Nickel Metallochaperone, UreE

scientific article published in Journal of Biological Chemistry

In vitro activation of urease apoprotein and role of UreD as a chaperone required for nickel metallocenter assembly.

scientific article

Inhibition of urease by bismuth(III): implications for the mechanism of action of bismuth drugs.

scientific article published on October 2006

Insight into the mechanism of an iron dioxygenase by resolution of steps following the FeIV=HO species

scientific article

Interconversion of two oxidized forms of taurine/alpha-ketoglutarate dioxygenase, a non-heme iron hydroxylase: evidence for bicarbonate binding

scholarly article

Interplay of metal ions and urease

scientific article

Intrinsic tryptophan fluorescence as a probe of metal and alpha-ketoglutarate binding to TfdA, a mononuclear non-heme iron dioxygenase

scientific article published in January 2003

Iron-containing urease in a pathogenic bacterium

scientific article

Kinetic and spectroscopic investigation of CoII, NiII, and N-oxalylglycine inhibition of the FeII/alpha-ketoglutarate dioxygenase, TauD.

scientific article published on 8 September 2005

Kinetic and structural characterization of urease active site variants

scientific article

Klebsiella aerogenes UreF: identification of the UreG binding site and role in enhancing the fidelity of urease activation

scientific article published on 6 March 2012

Klebsiella aerogenes urease gene cluster: sequence of ureD and demonstration that four accessory genes (ureD, ureE, ureF, and ureG) are involved in nickel metallocenter biosynthesis ⬇️

scientific article published on July 1, 1992

Lactate Racemase Nickel-Pincer Cofactor Operates by a Proton-Coupled Hydride Transfer Mechanism.

scientific article published on 28 February 2018

Lanthanide-dependent alcohol dehydrogenases require an essential aspartate residue for metal coordination and enzymatic function

scientific article published on 04 May 2020

METALLOPROTEINS. A tethered niacin-derived pincer complex with a nickel-carbon bond in lactate racemase

scientific article

Measuring the orientation of taurine in the active site of the non-heme Fe(II)/α-ketoglutarate-dependent taurine hydroxylase (TauD) using electron spin echo envelope modulation (ESEEM) spectroscopy

scientific article published on 29 August 2013

Mechanism of the 6-hydroxy-3-succinoyl-pyridine 3-monooxygenase flavoprotein from Pseudomonas putida S16.

scientific article

Mechanisms of metal ion incorporation into metalloproteins.

scientific article published on July 1990

Mechanisms of nickel toxicity in microorganisms

scientific article published on 28 July 2011

Metabolic versatility of prokaryotes for urea decomposition

scientific article published on May 2004

Metal Ion Interactions with Urease and UreD-Urease Apoproteins†

article

Metal and substrate binding to an Fe(II) dioxygenase resolved by UV spectroscopy with global regression analysis

scientific article

Metal ion dependence of recombinant Escherichia coli allantoinase

scientific article

Metal ligand substitution and evidence for quinone formation in taurine/alpha-ketoglutarate dioxygenase.

scientific article published on 3 February 2007

Methanogen factor 390 formation: species distribution, reversibility and effects of non-oxidative cellular stresses

article

Microbial ureases: significance, regulation, and molecular characterization

scientific article

Mutagenesis of Klebsiella aerogenes UreG to probe nickel binding and interactions with other urease-related proteins

scientific article

Mutational and Computational Evidence That a Nickel-Transfer Tunnel in UreD Is Used for Activation of Klebsiella aerogenes Urease

scientific article

Mössbauer and EPR studies of desulforedoxin from Desulfovibrio gigas ⬇️

scientific article published in Journal of Biological Chemistry

NADPH-dependent reductive ortho dehalogenation of 2,4-dichlorobenzoic acid in Corynebacterium sepedonicum KZ-4 and Coryneform bacterium strainNTB-1 via 2,4-dichlorobenzoyl coenzyme A

scientific article

New insights into acetone metabolism

scientific article published on December 2006

New metal cofactors and recent metallocofactor insights

scientific article published on 01 February 2019

Ni and CO: more surprises

article

Nickel enzymes in microbes

scientific article published on 01 June 1994

Nickel-dependent metalloenzymes

scientific article

Nickel-pincer cofactor biosynthesis involves LarB-catalyzed pyridinium carboxylation and LarE-dependent sacrificial sulfur insertion

scientific article published on 25 April 2016

Non-heme iron oxygenases

scientific article

O2- and alpha-ketoglutarate-dependent tyrosyl radical formation in TauD, an alpha-keto acid-dependent non-heme iron dioxygenase

scientific article published in February 2003

Oxidative demethylation by Escherichia coli AlkB directly reverts DNA base damage

scientific article (publication date: 12 September 2002)

Paramagnetic centers in the nickel-containing, deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum

scientific article published on January 1983

Partial purification of goat kidney beta-mannosidase

scientific article published on February 1988

Preliminary crystallographic studies of urease from jack bean and from Klebsiella aerogenes

scientific article published in October 1992

Probing the 2,4-dichlorophenoxyacetate/alpha-ketoglutarate dioxygenase substrate-binding site by site-directed mutagenesis and mechanism-based inactivation

scientific article published in August 2002

Probing the iron-substrate orientation for taurine/alpha-ketoglutarate dioxygenase using deuterium electron spin echo envelope modulation spectroscopy.

scientific article published in May 2007

Proteus mirabilis urease. Partial purification and inhibition by boric acid and boronic acids

scientific article published on March 1988

Pseudomonas aeruginosa 142 uses a three-component ortho-halobenzoate 1,2-dioxygenase for metabolism of 2,4-dichloro- and 2-chlorobenzoate

scientific article published on June 1994

Purification and activation properties of UreD-UreF-urease apoprotein complexes.

scientific article published on September 1996

Purification and characterization of 2,4-dichlorophenoxyacetate/alpha-ketoglutarate dioxygenase.

scientific article published on November 1993

Purification and characterization of Klebsiella aerogenes UreE protein: a nickel-binding protein that functions in urease metallocenter assembly.

scientific article

Purification and characterization of the FeII- and alpha-ketoglutarate-dependent xanthine hydroxylase from Aspergillus nidulans

scientific article published on 13 April 2007

Purification and characterization of the nickel-containing multicomponent urease from Klebsiella aerogenes

scientific article published in Journal of Biological Chemistry

Purification and properties of the Klebsiella aerogenes UreE metal-binding domain, a functional metallochaperone of urease

scientific article

Purification of a nickel-containing urease from the rumen anaerobe Selenomonas ruminantium

scientific article published in Journal of Biological Chemistry

Reduction of urease activity by interaction with the flap covering the active site

scientific article published on 30 January 2015

Regulation of gene expression and cellular localization of cloned Klebsiella aerogenes (K. pneumoniae) urease

article

Requirement of carbon dioxide for in vitro assembly of the urease nickel metallocenter.

scientific article

Resonance Raman studies of the iron(II)--alpha-keto acid chromophore in model and enzyme complexes

scientific article published in May 2001

Self-hydroxylation of taurine/alpha-ketoglutarate dioxygenase: evidence for more than one oxygen activation mechanism

scientific article published in December 2005

Site-directed Mutagenesis of 2,4-Dichlorophenoxyacetic Acid/α-Ketoglutarate Dioxygenase

scientific article published in Journal of Biological Chemistry

Site-directed mutagenesis of Klebsiella aerogenes urease: identification of histidine residues that appear to function in nickel ligation, substrate binding, and catalysis

scientific article

Site-directed mutagenesis of the Anabaena sp. strain PCC 7120 nitrogenase active site to increase photobiological hydrogen production

scientific article (publication date: October 2010)

Site-directed mutagenesis of the active site cysteine in Klebsiella aerogenes urease ⬇️

scientific article published on October 5, 1992

Steady-state and transient kinetic analyses of taurine/alpha-ketoglutarate dioxygenase: effects of oxygen concentration, alternative sulfonates, and active-site variants on the FeIV-oxo intermediate

scientific article

Stopped-Flow Kinetic Analysis ofEscherichia coliTaurine/α-Ketoglutarate Dioxygenase: Interactions with α-Ketoglutarate, Taurine, and Oxygen†

article

Strongly Coupled Redox-Linked Conformational Switching at the Active Site of the Non-Heme Iron-Dependent Dioxygenase, TauD

scientific article published on 06 September 2019

Structural Origin of the Large Redox-Linked Reorganization in the 2-Oxoglutarate Dependent Oxygenase, TauD

scientific article published on 11 September 2019

Structural basis for the enantiospecificities of R- and S-specific phenoxypropionate/alpha-ketoglutarate dioxygenases

scientific article

Structural insights into the catalytic mechanism of a sacrificial sulfur insertase of the N-type ATP pyrophosphatase family, LarE.

scientific article published on 7 August 2017

Structures and Mechanisms of the Non-Heme Fe(II)- and 2-Oxoglutarate-Dependent Ethylene-Forming Enzyme: Substrate Binding Creates a Twist.

scientific article

Substitution of the Urease Active Site Carbamate by Dithiocarbamate and Vanadate†

article

The AidB component of the Escherichia coli adaptive response to alkylating agents is a flavin-containing, DNA-binding protein

scientific article

The Escherichia coli alkylation response protein AidB is a redox partner of flavodoxin and binds RNA and acyl carrier protein

scientific article

The UreEF fusion protein provides a soluble and functional form of the UreF urease accessory protein

scientific article

The amino acid sequence of the nitrogenase iron protein from Azotobacter vinelandii

scientific article published in Journal of Biological Chemistry

The protein that binds to DNA base J in trypanosomatids has features of a thymidine hydroxylase.

scientific article published on 27 March 2007

The structure of urease activation complexes examined by flexibility analysis, mutagenesis, and small-angle X-ray scattering

scientific article published on 18 September 2008

Thermodynamics of Ni2+, Cu2+, and Zn2+ binding to the urease metallochaperone UreE.

scientific article

Trypanosoma brucei brucei: thymine 7-hydroxylase-like proteins

scientific article

Uncovering a superfamily of nickel-dependent hydroxyacid racemases and epimerases

scientific article published on 22 October 2020

UreE Stimulation of GTP-Dependent Urease Activation in the UreD-UreF-UreG-urease Apoprotein Complex†

article

Urease activity in the crystalline state

scientific article published on October 1995

Use of 4-Nitrophenoxyacetic Acid for Detection and Quantification of 2,4-Dichlorophenoxyacetic Acid (2,4-D)/(alpha)-Ketoglutarate Dioxygenase Activity in 2,4-D-Degrading Microorganisms.

scientific article

X-ray absorption spectroscopic analysis of Fe(II) and Cu(II) forms of a herbicide-degrading α-ketoglutarate dioxygenase

article

[33] Separation of flavins and flavin analogs by high-performance liquid chromatography

article

tfdA-like genes in 2,4-dichlorophenoxyacetic acid-degrading bacteria belonging to the Bradyrhizobium-Agromonas-Nitrobacter-Afipia cluster in alpha-Proteobacteria

scientific article

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