List of works - Nediljko Budisa

Adding New Tools to the Arsenal of Expressed Protein Ligation

scientific article published on 01 September 2004

Alternative Biochemistries for Alien Life: Basic Concepts and Requirements for the Design of a Robust Biocontainment System in Genetic Isolation

article

Amide rotation trajectories probed by symmetry

scientific article published on 2 August 2017

Aminotryptophan-containing barstar: structure--function tradeoff in protein design and engineering with an expanded genetic code.

scientific article published on 10 May 2006

An expanded genetic code for probing the role of electrostatics in enzyme catalysis by vibrational Stark spectroscopy

scientific article published on 13 February 2017

Anticipating alien cells with alternative genetic codes: away from the alanine world!

scientific article published on 03 July 2019

Applying γ-substituted prolines in the foldon peptide: polarity contradicts preorganization

scientific article published on 16 December 2014

Atomic Mutations at the Single Tryptophan Residue of Human Recombinant Annexin V: Effects on Structure, Stability, and Activity‡

scientific article published on 01 August 1999

Atomic mutations in annexin V. Thermodynamic studies of isomorphous protein variants

scientific article published on 01 April 1998

Azatryptophans as tools to study polarity requirements for folding of green fluorescent protein

scientific article published on October 2010

Azatryptophans endow proteins with intrinsic blue fluorescence

scholarly article

Bilayer thickness determines the alignment of model polyproline helices in lipid membranes

scientific article published on 01 October 2019

Bioconjugation ofl-3,4-Dihydroxyphenylalanine Containing Protein with a Polysaccharide

scientific article published on 04 March 2011

Bioincorporation of telluromethionine into proteins: a promising new approach for X-ray structure analysis of proteins.

scientific article published in July 1997

Blue fluorescent amino acids as in vivo building blocks for proteins

scientific article published in February 2010

Cell-free expression with the toxic amino acid canavanine

scientific article

Chemical Evolution of a Bacterial Proteome

scientific article published on July 2015

Comparative effects of trifluoromethyl- and methyl-group substitutions in proline

Computational Aminoacyl-tRNA Synthetase Library Design for Photocaged Tyrosine. ⬇️

scientific article published on 11 May 2019

Congeneric bio-adhesive mussel foot proteins designed by modified prolines revealed a chiral bias in unnatural translation

scientific article published on 20 April 2012

Congeneric lantibiotics from ribosomal in vivo peptide synthesis with noncanonical amino acids

scientific article published on 30 November 2011

Conjugation of proteins by installing BIO-orthogonally reactive groups at their N-termini

scientific article

Construction of a polyproline structure with hydrophobic exterior using octahydroindole-2-carboxylic acid

scientific article published on 13 December 2016

Convenient syntheses of homopropargylglycine

scientific article published in October 2008

Coupling bioorthogonal chemistries with artificial metabolism: intracellular biosynthesis of azidohomoalanine and its incorporation into recombinant proteins.

scientific article published on 15 January 2014

Coupling genetic code expansion and metabolic engineering for synthetic cells

scientific article published on 23 February 2017

Covalent attachment of cyclic TAT peptides to GFP results in protein delivery into live cells with immediate bioavailability

scientific article published on 17 December 2014

Crystallographic evidence for isomeric chromophores in 3-fluorotyrosyl-green fluorescent protein

scientific article

Deciphering the Fluorine Code-The Many Hats Fluorine Wears in a Protein Environment.

scientific article published on 12 August 2017

Dendronylation: Residue-specific chemoselective attachment of oligoglycerol dendrimers on proteins with noncanonical amino acids

scientific article published on 25 September 2015

Design of S-Allylcysteine in Situ Production and Incorporation Based on a Novel Pyrrolysyl-tRNA Synthetase Variant

scientific article

Design of anti- and pro-aggregation variants to assess the effects of methionine oxidation in human prion protein

scholarly article

Design of protein congeners containing β-cyclopropylalanine.

scientific article published in October 2012

Designing novel spectral classes of proteins with a tryptophan-expanded genetic code

scientific article

Discharging tRNAs: a tug of war between translation and detoxification in Escherichia coli.

scientific article published on 9 August 2016

Docking of tryptophanyl [corrected tryptophan] analogs to trytophanyl-tRNA synthetase: implications for non-canonical amino acid incorporations

scientific article published in September 2008

Efficient N-Terminal Glycoconjugation of Proteins by the N-End Rule

scientific article published on 01 May 2008

Efforts towards the design of 'teflon' proteins: in vivo translation with trifluorinated leucine and methionine analogues.

scientific article published in October 2004

Energetic contribution to both acidity and conformational stability in peptide models

Engineering protein sequence composition for folding robustness renders efficient noncanonical amino acid incorporations

scientific article published on December 2010

Engineering the Genetic Code

article

Enhancing the thermal stability of a single-chain Fv fragment by in vivo global fluorination of the proline residues

scientific article published on 19 November 2010

Entropic Contribution of Elongation Factor P to Proline Positioning at the Catalytic Center of the Ribosome

scientific article published on 18 September 2015

Evaluation of bicinchoninic acid as a ligand for copper(i)-catalyzed azide–alkyne bioconjugations

scientific article published on 23 July 2012

Expanded genetic code for the engineering of ribosomally synthetized and post-translationally modified peptide natural products (RiPPs).

scientific article published on 25 March 2013

Expanding and engineering the genetic code in a single expression experiment

scientific article published on 18 January 2011

Expanding the DOPA Universe with Genetically Encoded, Mussel-Inspired Bioadhesives for Material Sciences and Medicine

scientific article published on 24 June 2019

Expanding the Scope of Orthogonal Translation with Pyrrolysyl-tRNA Synthetases Dedicated to Aromatic Amino Acids

scientific article published on 25 September 2020

Expanding the genetic code of Saccharomyces cerevisiae with methionine analogues

scientific article

Expansion of the genetic code enables design of a novel "gold" class of green fluorescent proteins

scientific article (publication date: 16 May 2003)

Exploring hydrophobicity limits of polyproline helix with oligomeric octahydroindole-2-carboxylic acid.

scientific article published on 27 March 2018

Expression, purification, characterization, and X-ray analysis of selenomethionine 215 variant of leukocyte collagenase.

scientific article published in August 1997

Fine tuning the N-terminal residue excision with methionine analogues

scientific article published on January 2009

Fluorine-rich planetary environments as possible habitats for life

scientific article published on 18 August 2014

Genetically Encoded Photocrosslinkers as Molecular Probes To Study G‐Protein‐Coupled Receptors (GPCRs) ⬇️

scientific article published on November 30, 2011

Gold fluorescent annexin A5 as a novel apoptosis detection tool

scientific article

Hydrolysis, polarity, and conformational impact of C-terminal partially fluorinated ethyl esters in peptide models

scientific article published on 16 November 2017

Improved method to retain cytosolic reporter protein fluorescence while staining for nuclear proteins

scientific article published on 19 February 2014

In Vivo Chemoenzymatic Control of N-Terminal Processing in Recombinant Human Epidermal Growth Factor

scientific article published on 01 December 2007

In vivo double and triple labeling of proteins using synthetic amino acids

scientific article published on July 2010

In vivo engineering of proteins with nitrogen-containing tryptophan analogs

scientific article published on December 2006

In vivo incorporation of multiple noncanonical amino acids into proteins

scientific article published on 25 February 2011

In-Cell Synthesis of Bioorthogonal Alkene Tag S-Allyl-Homocysteine and Its Coupling with Reprogrammed Translation ⬇️

scientific article published on 09 May 2019

Incorporation of Amino Acids with Long-Chain Terminal Olefins into Proteins.

scientific article published on 29 February 2016

Incorporation of beta-selenolo[3,2-b]pyrrolyl-alanine into proteins for phase determination in protein X-ray crystallography.

scientific article published in June 2001

Inside Cover: Chemical Evolution of a Bacterial Proteome (Angew. Chem. Int. Ed. 34/2015)

Intracellular uptake and inhibitory activity of aromatic fluorinated amino acids in human breast cancer cells.

scientific article

Laboratory evolution of Escherichia coli enables life based on fluorinated amino acids

scholarly article published 10 June 2019

Lipase Congeners Designed by Genetic Code Engineering

Long-Range Modulations of Electric Fields in Proteins

scientific article published on 28 August 2018

Molecular cloning and sequence analysis of a novel zinc-metalloprotease gene from the Salinivibrio sp. strain AF-2004 and its extracellular expression in E. coli.

scientific article

Multiomics Analysis Provides Insight into the Laboratory Evolution of Escherichia coli toward the Metabolic Usage of Fluorinated Indoles

scientific article published in 2020

Natural history and experimental evolution of the genetic code

scientific article published on February 2007

Noninvasive Tracing of Recombinant Proteins with “Fluorophenylalanine-Fingers”

scientific article published on 01 August 2000

On the Road towards Chemically Modified Organisms Endowed with a Genetic Firewall ⬇️

scientific article published on June 24, 2011

One-Pot Synthesis of Unprotected Anomeric Glycosyl Thiols in Water for Glycan Ligation Reactions with Highly Functionalized Sugars

scientific article published on 11 November 2016

Organic fluorine as a polypeptide building element: in vivo expression of fluorinated peptides, proteins and proteomes

scientific article published on September 2012

Orthogonal dual-modification of proteins for the engineering of multivalent protein scaffolds

scientific article

Orthogonal translation meets electron transfer: in vivo labeling of cytochrome c for probing local electric fields

scientific article published on 23 February 2015

Orthogonal translation with 5‐cyanotryptophan as an infrared probe for local structural information, electrostatics, and hydrogen bonding

scientific article published in 2023

Parallel incorporation of different fluorinated amino acids: on the way to "teflon" proteins.

scientific article

Performance analysis of orthogonal pairs designed for an expanded eukaryotic genetic code

scientific article

Photochemical formation of quinone methides from peptides containing modified tyrosine

scientific article

Photostability of green and yellow fluorescent proteins with fluorinated chromophores, investigated by fluorescence correlation spectroscopy

scientific article published on 23 April 2008

Polyoxometalate-stabilized, water dispersible Fe2Pt magnetic nanoparticles

scientific article published on 01 March 2013

Probing the role of tryptophans in Aequorea victoria green fluorescent proteins with an expanded genetic code.

scientific article published in February 2004

Prolegomena to future experimental efforts on genetic code engineering by expanding its amino acid repertoire

scientific article

Promotion of the collagen triple helix in a hydrophobic environment

scientific article published on 01 February 2019

Prospects of In vivo Incorporation of Non-canonical Amino Acids for the Chemical Diversification of Antimicrobial Peptides

scientific article (publication date: 2017)

Protein iodination by click chemistry

scientific article published in May 2009

Proteins with beta-(thienopyrrolyl)alanines as alternative chromophores and pharmaceutically active amino acids

scientific article

Recent advances in genetic code engineering in Escherichia coli ⬇️

scientific article published on January 9, 2012

Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System

scientific article (publication date: August 2016)

Residue-specific bioincorporation of non-natural, biologically active amino acids into proteins as possible drug carriers: Structure and stability of the per -thiaproline mutant of annexin V ⬇️

scientific article published on January 20, 1998

Residue-specific global fluorination of Candida antarctica lipase B in Pichia pastoris

scientific article published on 29 April 2010

Secretion of recombinant archeal lipase mediated by SVP2 signal peptide in Escherichia coli and its optimization by response surface methodology

scientific article

Sense codon emancipation for proteome-wide incorporation of noncanonical amino acids: rare isoleucine codon AUA as a target for genetic code expansion

scientific article

Site-Resolved Observation of Vibrational Energy Transfer Using a Genetically Encoded Ultrafast Heater

scientific article published on 06 February 2019

Site-Specific Chemoselective Pyrrolysine Analogues Incorporation Using the Cell-Free Protein Synthesis System

scientific article published on 28 January 2019

Site-directed and global incorporation of orthogonal and isostructural noncanonical amino acids into the ribosomal lasso peptide capistruin

scientific article published on 11 December 2014

Site-selective modification of proteins for the synthesis of structurally defined multivalent scaffolds

scientific article published on 09 November 2011

Site-specific conjugation of 8-ethynyl-BODIPY to a protein by [2 + 3] cycloaddition

scientific article published in June 2015

Slow exchange in the chromophore of a green fluorescent protein variant

scientific article published in July 2002

Strategy for Enhancement of (13)C-Photo-CIDNP NMR Spectra by Exploiting Fractional (13)C-Labeling of Tryptophan

scientific article published on 5 August 2015

Structural and spectral response of Aequorea victoria green fluorescent proteins to chromophore fluorination.

scientific article

Supercritical carbon dioxide and its potential as a life-sustaining solvent in a planetary environment

scientific article published on 8 August 2014

Synthesis of a Photo-Caged DOPA Derivative by Selective Alkylation of 3,4-Dihydroxybenzaldehyde

Synthetic Biology of Proteins: Tuning GFPs Folding and Stability with Fluoroproline

scientific article

Synthetic biology of protein folding.

scientific article

The Alanine World Model for the Development of the Amino Acid Repertoire in Protein Biosynthesis ⬇️

scientific article published on 05 November 2019

Toward intrinsically colored peptides: Synthesis and investigation of the spectral properties of methylated azatryptophans in tryptophan-cage mutants

scientific article published on 6 August 2015

Towards Biocontained Cell Factories: An Evolutionarily Adapted Escherichia coli Strain Produces a New-to-nature Bioactive Lantibiotic Containing Thienopyrrole-Alanine

scientific article published on 16 September 2016

Transmembrane Polyproline Helix

scientific article published on 11 April 2018

Xenobiology: A Journey towards Parallel Life Forms

scientific article published on 22 April 2020

Xenomicrobiology: a roadmap for genetic code engineering

scientific article

cis-trans-Amide isomerism of the 3,4-dehydroproline residue, the 'unpuckered' proline

scientific article

γ-(S)-Trifluoromethyl proline: evaluation as a structural substitute of proline for solid state (19)F-NMR peptide studies.

scientific article

List of works by Nediljko Budisa

Adding New Tools to the Arsenal of Expressed Protein Ligation

Alternative Biochemistries for Alien Life: Basic Concepts and Requirements for the Design of a Robust Biocontainment System in Genetic Isolation

Amide rotation trajectories probed by symmetry

Aminotryptophan-containing barstar: structure--function tradeoff in protein design and engineering with an expanded genetic code.

An expanded genetic code for probing the role of electrostatics in enzyme catalysis by vibrational Stark spectroscopy

Anticipating alien cells with alternative genetic codes: away from the alanine world!

Applying γ-substituted prolines in the foldon peptide: polarity contradicts preorganization

Atomic Mutations at the Single Tryptophan Residue of Human Recombinant Annexin V: Effects on Structure, Stability, and Activity‡

Atomic mutations in annexin V. Thermodynamic studies of isomorphous protein variants

Azatryptophans as tools to study polarity requirements for folding of green fluorescent protein

Azatryptophans endow proteins with intrinsic blue fluorescence

Bilayer thickness determines the alignment of model polyproline helices in lipid membranes

Bioconjugation ofl-3,4-Dihydroxyphenylalanine Containing Protein with a Polysaccharide

Bioincorporation of telluromethionine into proteins: a promising new approach for X-ray structure analysis of proteins.

Blue fluorescent amino acids as in vivo building blocks for proteins

Cell-free expression with the toxic amino acid canavanine

Chemical Evolution of a Bacterial Proteome

Comparative effects of trifluoromethyl- and methyl-group substitutions in proline

Computational Aminoacyl-tRNA Synthetase Library Design for Photocaged Tyrosine. ⬇️

Congeneric bio-adhesive mussel foot proteins designed by modified prolines revealed a chiral bias in unnatural translation

Congeneric lantibiotics from ribosomal in vivo peptide synthesis with noncanonical amino acids

Conjugation of proteins by installing BIO-orthogonally reactive groups at their N-termini

Construction of a polyproline structure with hydrophobic exterior using octahydroindole-2-carboxylic acid

Convenient syntheses of homopropargylglycine

Coupling bioorthogonal chemistries with artificial metabolism: intracellular biosynthesis of azidohomoalanine and its incorporation into recombinant proteins.

Coupling genetic code expansion and metabolic engineering for synthetic cells

Covalent attachment of cyclic TAT peptides to GFP results in protein delivery into live cells with immediate bioavailability

Crystallographic evidence for isomeric chromophores in 3-fluorotyrosyl-green fluorescent protein

Deciphering the Fluorine Code-The Many Hats Fluorine Wears in a Protein Environment.

Dendronylation: Residue-specific chemoselective attachment of oligoglycerol dendrimers on proteins with noncanonical amino acids

Design of S-Allylcysteine in Situ Production and Incorporation Based on a Novel Pyrrolysyl-tRNA Synthetase Variant

Design of anti- and pro-aggregation variants to assess the effects of methionine oxidation in human prion protein

Design of protein congeners containing β-cyclopropylalanine.

Designing novel spectral classes of proteins with a tryptophan-expanded genetic code

Discharging tRNAs: a tug of war between translation and detoxification in Escherichia coli.

Docking of tryptophanyl [corrected tryptophan] analogs to trytophanyl-tRNA synthetase: implications for non-canonical amino acid incorporations

Efficient N-Terminal Glycoconjugation of Proteins by the N-End Rule

Efforts towards the design of 'teflon' proteins: in vivo translation with trifluorinated leucine and methionine analogues.

Energetic contribution to both acidity and conformational stability in peptide models

Engineering protein sequence composition for folding robustness renders efficient noncanonical amino acid incorporations

Engineering the Genetic Code

Enhancing the thermal stability of a single-chain Fv fragment by in vivo global fluorination of the proline residues

Entropic Contribution of Elongation Factor P to Proline Positioning at the Catalytic Center of the Ribosome

Evaluation of bicinchoninic acid as a ligand for copper(i)-catalyzed azide–alkyne bioconjugations

Expanded genetic code for the engineering of ribosomally synthetized and post-translationally modified peptide natural products (RiPPs).

Expanding and engineering the genetic code in a single expression experiment

Expanding the DOPA Universe with Genetically Encoded, Mussel-Inspired Bioadhesives for Material Sciences and Medicine

Expanding the Scope of Orthogonal Translation with Pyrrolysyl-tRNA Synthetases Dedicated to Aromatic Amino Acids

Expanding the genetic code of Saccharomyces cerevisiae with methionine analogues

Expansion of the genetic code enables design of a novel "gold" class of green fluorescent proteins

Exploring hydrophobicity limits of polyproline helix with oligomeric octahydroindole-2-carboxylic acid.

Expression, purification, characterization, and X-ray analysis of selenomethionine 215 variant of leukocyte collagenase.

Fine tuning the N-terminal residue excision with methionine analogues

Fluorine-rich planetary environments as possible habitats for life

Genetically Encoded Photocrosslinkers as Molecular Probes To Study G‐Protein‐Coupled Receptors (GPCRs) ⬇️

Gold fluorescent annexin A5 as a novel apoptosis detection tool

Hydrolysis, polarity, and conformational impact of C-terminal partially fluorinated ethyl esters in peptide models

Improved method to retain cytosolic reporter protein fluorescence while staining for nuclear proteins

In Vivo Chemoenzymatic Control of N-Terminal Processing in Recombinant Human Epidermal Growth Factor

In vivo double and triple labeling of proteins using synthetic amino acids

In vivo engineering of proteins with nitrogen-containing tryptophan analogs

In vivo incorporation of multiple noncanonical amino acids into proteins

In-Cell Synthesis of Bioorthogonal Alkene Tag S-Allyl-Homocysteine and Its Coupling with Reprogrammed Translation ⬇️

Incorporation of Amino Acids with Long-Chain Terminal Olefins into Proteins.

Incorporation of beta-selenolo[3,2-b]pyrrolyl-alanine into proteins for phase determination in protein X-ray crystallography.

Inside Cover: Chemical Evolution of a Bacterial Proteome (Angew. Chem. Int. Ed. 34/2015)

Intracellular uptake and inhibitory activity of aromatic fluorinated amino acids in human breast cancer cells.

Laboratory evolution of Escherichia coli enables life based on fluorinated amino acids

Lipase Congeners Designed by Genetic Code Engineering

Long-Range Modulations of Electric Fields in Proteins

Molecular cloning and sequence analysis of a novel zinc-metalloprotease gene from the Salinivibrio sp. strain AF-2004 and its extracellular expression in E. coli.

Multiomics Analysis Provides Insight into the Laboratory Evolution of Escherichia coli toward the Metabolic Usage of Fluorinated Indoles

Natural history and experimental evolution of the genetic code

Noninvasive Tracing of Recombinant Proteins with “Fluorophenylalanine-Fingers”

On the Road towards Chemically Modified Organisms Endowed with a Genetic Firewall ⬇️

One-Pot Synthesis of Unprotected Anomeric Glycosyl Thiols in Water for Glycan Ligation Reactions with Highly Functionalized Sugars

Organic fluorine as a polypeptide building element: in vivo expression of fluorinated peptides, proteins and proteomes

Orthogonal dual-modification of proteins for the engineering of multivalent protein scaffolds

Orthogonal translation meets electron transfer: in vivo labeling of cytochrome c for probing local electric fields