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List of works by Loredano Pollegioni

A biosensor for all D-amino acids using evolved D-amino acid oxidase

scientific article published on 8 June 2008

A comprehensive practical laboratory course on protein engineering: Evolution of a glycine oxidase variant active on the herbicide glyphosate

scientific article published on 24 August 2019

A novel, simple screening method for investigating the properties of lignin oxidative activity

scientific article published on 19 October 2016

A study on apoenzyme from Rhodotorula gracilis D-amino acid oxidase

scientific article published on 01 April 1991

Advances in Enzymatic Synthesis of D-Amino Acids

scientific article published on 01 May 2020

Age-Related Changes in D-Aspartate Oxidase Promoter Methylation Control Extracellular D-Aspartate Levels and Prevent Precocious Cell Death during Brain Aging.

scientific article published on March 2016

Aminoacetone oxidase from Streptococcus oligofermentans belongs to a new three-domain family of bacterial flavoproteins

scientific article

Analyzing the D-amino acid content in biological samples by engineered enzymes

article

Antibacterial Properties of D-Amino Acid Oxidase: Impact on the Food Industry

scientific article published on 03 December 2019

Application of a rapid HILIC-UV method for synthesis optimization and stability studies of immunogenic neo-glycoconjugates

scientific article published on 28 March 2017

Assays of D-amino acid oxidases

scientific article

Astroglial d-serine is the endogenous co-agonist at the presynaptic NMDA receptor in rat entorhinal cortex

scientific article published on 18 April 2014

Bacterial Nanocellulose and Its Surface Modification by Glycidyl Methacrylate and Ethylene Glycol Dimethacrylate. Incorporation of Vancomycin and Ciprofloxacin

scientific article published on 22 November 2019

Binding Residence Time through Scaled Molecular Dynamics: A Prospective Application to hDAAO Inhibitors

scientific article published on 05 November 2018

Biochemical Properties of Human D-Amino Acid Oxidase.

scientific article published on 15 December 2017

Biosensors for D-Amino Acids: Detection Methods and Applications ⬇️

scientific article published on 27 June 2020

Breaking the mirror: l-Amino acid deaminase, a novel stereoselective biocatalyst

scientific article published on 03 August 2017

Catalytic and redox properties of glycine oxidase from Bacillus subtilis.

scientific article published on 28 February 2009

Catalytic properties of D-amino acid oxidase in cephalosporin C bioconversion: a comparison between proteins from different sources.

scientific article published in March 2004

Cellular prion protein neither binds to alpha-synuclein oligomers nor mediates their detrimental effects

scientific article published on 01 February 2019

Cephalosporin C acylase: dream and(/or) reality.

scientific article published on 16 February 2013

Characterization of VanYn, a novel D,D-peptidase/D,D-carboxypeptidase involved in glycopeptide antibiotic resistance in Nonomuraea sp. ATCC 39727.

scientific article published on 2 August 2012

Characterization of human DAAO variants potentially related to an increased risk of schizophrenia.

scientific article published on 5 December 2012

Characterization of the covalently bound anionic flavin radical in monoamine oxidase a by electron paramagnetic resonance.

scientific article published on 29 November 2007

Cholesterol oxidase from Brevibacterium sterolicum. The relationship between covalent flavinylation and redox properties.

scientific article published on 28 February 2001

Cholesterol oxidase: a model flavoprotein oxidase and a biotechnological tool.

scientific article published on 16 October 2009

Circuit-specific changes in D-serine-dependent activation of the N-methyl-D-aspartate receptor in the aging hippocampus.

scientific article published on 20 August 2014

Contribution of serine racemase/d-serine pathway to neuronal apoptosis.

scientific article published on 5 June 2012

Contribution of the dimeric state to the thermal stability of the flavoprotein D-amino acid oxidase.

scientific article

Conversion of the dimeric D-amino acid oxidase from Rhodotorula gracilis to a monomeric form. A rational mutagenesis approach.

scientific article

Covalent enzyme immobilization by poly(ethylene glycol) diglycidyl ether (PEGDE) for microelectrode biosensor preparation

scientific article published on 06 April 2011

D-Serine and Glycine Differentially Control Neurotransmission during Visual Cortex Critical Period

scientific article

D-amino acid oxidase inhibitors as a novel class of drugs for schizophrenia therapy.

scientific article

D-amino acid oxidase-nanoparticle system: a potential novel approach for cancer enzymatic therapy.

scientific article

D-amino acids in foods

scientific article published on 12 December 2019

Decreased free d-aspartate levels are linked to enhanced d-aspartate oxidase activity in the dorsolateral prefrontal cortex of schizophrenia patients

scientific article

Different recombinant forms of polyphenol oxidase A, a laccase from Marinomonas mediterranea.

scientific article published on 2 April 2016

Direct chromatographic methods for enantioresolution of amino acids: recent developments

scientific article published on 15 July 2020

Disk-Shaped Amperometric Enzymatic Biosensor for in Vivo Detection of d-serine

scientific article published in 2014

Dissecting the structural determinants of the stability of cholesterol oxidase containing covalently bound flavin

scientific article published on 7 April 2005

Editorial: Bioscience of D-amino Acid Oxidase From Biochemistry to Pathophysiology

scientific article published on 28 November 2018

Effect of ligand binding on human D-amino acid oxidase: implications for the development of new drugs for schizophrenia treatment

scientific article

Elucidating the role of the pLG72 R30K substitution in schizophrenia susceptibility

scientific article published on 6 February 2017

Engineering methionine γ-lyase from Citrobacter freundii for anticancer activity

scientific article published on 27 September 2018

Engineering substrate promiscuity in halophilic alcohol dehydrogenase (HvADH2) by in silico design.

scientific article published on 30 November 2017

Engineering the substrate specificity of D-amino-acid oxidase.

scientific article published on 20 May 2002

Enzymatic transformation of aflatoxin B1 by Rh_DypB peroxidase and characterization of the reaction products

scientific article published on 25 February 2020

Epitope and affinity determination of recombinant Mycobacterium tuberculosis Ag85B antigen towards anti-Ag85 antibodies using proteolytic affinity-mass spectrometry and biosensor analysis

scientific article published on 29 November 2018

Evaluation of D-amino acid oxidase from Rhodotorula gracilis for the production of alpha-keto acids: a reactor system

scientific article published on 01 December 1994

Evidence for the interaction of D-amino acid oxidase with pLG72 in a glial cell line

scientific article

Evolution of an acylase active on cephalosporin C

scientific article published on 31 October 2005

Evolution of histamine oxidase activity for biotechnological applications

scientific article published on 31 August 2013

Expression in Escherichia coli and in vitro refolding of the human protein pLG72.

scientific article

Expression in Escherichia coli of the catalytic domain of human proline oxidase.

scientific article

Expression of an evolved engineered variant of a bacterial glycine oxidase leads to glyphosate resistance in alfalfa.

scientific article published on 4 June 2014

FAD binding in glycine oxidase from Bacillus subtilis.

scientific article published on 12 September 2009

Free d-aspartate triggers NMDA receptor-dependent cell death in primary cortical neurons and perturbs JNK activation, Tau phosphorylation, and protein SUMOylation in the cerebral cortex of mice lacking d-aspartate oxidase activity

scientific article published on 26 February 2019

G72 primate-specific gene: a still enigmatic element in psychiatric disorders

scientific article published on 25 February 2016

Glial D-serine gates NMDA receptors at excitatory synapses in prefrontal cortex

scientific article published on 20 June 2011

Glycosylation of Recombinant Antigenic Proteins from Mycobacterium tuberculosis: In Silico Prediction of Protein Epitopes and Ex Vivo Biological Evaluation of New Semi-Synthetic Glycoconjugates.

scientific article

Glyphosate Resistance by Engineering the Flavoenzyme Glycine Oxidase

scientific article

High-Throughput Screening Strategy Identifies Allosteric, Covalent Human D-Amino Acid Oxidase Inhibitor

scientific article published on 21 August 2015

Human D-Amino Acid Oxidase: Structure, Function, and Regulation

scientific article published on 28 November 2018

Human d-amino acid oxidase: The inactive G183R variant

scientific article published on 21 December 2017

Identification of a Reactive Cysteine in the Flavin-Binding Domain ofRhodotorula gracilisd-Amino Acid Oxidase

scientific article published on 01 July 1997

Identity of endogenous NMDAR glycine site agonist in amygdala is determined by synaptic activity level ⬇️

scientific article published on January 1, 2013

Identity of the NMDA receptor coagonist is synapse specific and developmentally regulated in the hippocampus.

scientific article

Immunochemical studies on Rhodotorula gracilis D-amino acid oxidase

scientific article published on 01 March 1991

In vivo D-serine hetero-exchange through alanine-serine-cysteine (ASC) transporters detected by microelectrode biosensors.

scientific article

Insight into conformational modification of alpha-synuclein in the presence of neuronal whole cells and of their isolated membranes

scientific article

Investigating brain d-serine: Advocacy for good practices

scientific article published on 14 February 2019

Investigating the role of active site residues of Rhodotorula gracilis D-amino acid oxidase on its substrate specificity

scientific article published on 27 November 2006

Is rat an appropriate animal model to study the involvement of D-serine catabolism in schizophrenia? Insights from characterization of D-amino acid oxidase

scientific article published on 10 October 2011

Isolation and characterization of a heterologously expressed bacterial laccase from the anaerobe Geobacter metallireducens

scientific article

Kinetic mechanisms of cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum

scientific article published on 01 August 1999

L-amino acid oxidase as biocatalyst: a dream too far?

scientific article

L-serine synthesis via the phosphorylated pathway in humans

scientific article published on 27 June 2020

Lignin-degrading enzymes.

scientific article published on 20 February 2015

Liquid chromatography–mass spectrometry structural characterization of neo glycoproteins aiding the rational design and synthesis of a novel glycovaccine for protection against tuberculosis

scientific article published on 22 September 2014

Metabolic resistance of the D-peptide RD2 developed for direct elimination of amyloid-β oligomers

scientific article published on 05 April 2019

Metabolism of the neuromodulator D-serine.

scientific article

Modulating D-amino acid oxidase substrate specificity: production of an enzyme for analytical determination of all D-amino acids by directed evolution.

scientific article published in June 2004

Molecular basis of glyphosate resistance-different approaches through protein engineering

scientific article

Molecular insights into the role of the polyalanine region in mediating PHOX2B aggregation

scientific article published on 16 April 2019

New biotech applications from evolved D-amino acid oxidases.

scientific article published on 10 March 2011

New synthesis and biodistribution of the D-amino acid oxidase-magnetic nanoparticle system.

scientific article published on 11 September 2015

Novel biosensors based on optimized glycine oxidase.

scientific article published in July 2014

Novel human ᴅ-amino acid oxidase inhibitors stabilize an active-site lid-open conformation

scientific article

O2 reactivity of flavoproteins: dynamic access of dioxygen to the active site and role of a H+ relay system in D-amino acid oxidase

scientific article

Olanzapine, but not clozapine, increases glutamate release in the prefrontal cortex of freely moving mice by inhibiting D-aspartate oxidase activity

scientific article published on 10 April 2017

On the holoenzyme reconstitution process in native and truncated Rhodotorula gracilis D-amino acid oxidase

scientific article published on 01 August 1996

On the mechanism of D-amino acid oxidase. Structure/linear free energy correlations and deuterium kinetic isotope effects using substituted phenylglycines

scientific article published on 01 February 1997

On the mechanism of Rhodotorula gracilis D-amino acid oxidase: role of the active site serine 335.

scientific article published in October 2004

On the reaction of d-amino acid oxidase with dioxygen: O2 diffusion pathways and enhancement of reactivity

scientific article published on 23 December 2010

One single method to produce native and Tat-fused recombinant human α-synuclein in Escherichia coli.

scientific article

Optimization of glutaryl-7-aminocephalosporanic acid acylase expression in E. coli.

scientific article published on 28 May 2008

Optimizing Escherichia coli as a protein expression platform to produce Mycobacterium tuberculosis immunogenic proteins

scientific article

Optimizing HIV-1 protease production in Escherichia coli as fusion protein.

scientific article published on 30 June 2011

Overexpression in Escherichia coli of a recombinant chimeric Rhodotorula gracilis d-amino acid oxidase

scientific article published on 01 November 1998

Overexpression of a bacterial chymotrypsin: application for L-amino acid ester hydrolysis.

scientific article

Overexpression of a recombinant wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli.

scientific article published in March 2002

P53 family members modulate the expression of PRODH, but not PRODH2, via intronic p53 response elements.

scientific article

PEG-DAAO conjugate: A promising tool for cancer therapy optimized by protein engineering

scientific article published on 06 November 2019

Physiological functions of D-amino acid oxidases: from yeast to humans

scientific article

Production and characterization of a novel antifungal chitinase identified by functional screening of a suppressive-soil metagenome

scientific article

Production of recombinant cholesterol oxidase containing covalently bound FAD in Escherichia coli

scientific article

Properties of l-amino acid deaminase: En route to optimize bioconversion reactions

scientific article published on 20 January 2019

Purification of Rhodotorula gracilis D-amino acid oxidase

scientific article published on 01 April 1992

Rational design, preparation and characterization of recombinant Ag85B variants and their glycoconjugates with T-cell antigenic activity against <i>Mycobacterium tuberculosis</i>

scientific article published on 26 June 2018

Recombinant human Tat-Hsp70-2: A tool for neuroprotection

scientific article published on 9 July 2016

Recombinant production of eight human cytosolic aminotransferases and assessment of their potential involvement in glyoxylate metabolism.

scientific article published on 13 August 2009

Redox potentials and their pH dependence of D-amino-acid oxidase of Rhodotorula gracilis and Trigonopsis variabilis

scientific article published on 01 November 2000

Reduced D-serine levels in the nucleus accumbens of cocaine-treated rats hinder the induction of NMDA receptor-dependent synaptic plasticity

scientific article published on 20 March 2013

Regulating levels of the neuromodulator d-serine in human brain: structural insight into pLG72 and d-amino acid oxidase interaction

scientific article published on 12 July 2016

Relevance of the flavin binding to the stability and folding of engineered cholesterol oxidase containing noncovalently bound FAD.

scientific article published on 24 January 2008

Relevance of weak flavin binding in human D-amino acid oxidase

scientific article

Role of arginine 285 in the active site of Rhodotorula gracilis D-amino acid oxidase. A site-directed mutagenesis study.

scientific article published on August 2000

Serine racemase: a key player in apoptosis and necrosis

scientific article

Single-batch, homogeneous phase depolymerization of cellulose catalyzed by a monocomponent endocellulase in ionic liquid [BMIM][Cl]

Special Issue on "Cofactor-dependent proteins: Evolution, chemical diversity and bio-applications".

scientific article published on 10 June 2015

Specificity and kinetics of Rhodotorula gracilis D-amino acid oxidase

scientific article published on 01 March 1992

Strategic manipulation of an industrial biocatalyst--evolution of a cephalosporin C acylase

scientific article published on 22 April 2014

Structural, kinetic, and pharmacodynamic mechanisms of D-amino acid oxidase inhibition by small molecules

scientific article

Structure and kinetic properties of human d-aspartate oxidase, the enzyme-controlling d-aspartate levels in brain

scientific article published on 29 November 2019

Structure of a class III engineered cephalosporin acylase: comparisons with class I acylase and implications for differences in substrate specificity and catalytic activity

scientific article

Structure-Function Relationships in l-Amino Acid Deaminase, a Flavoprotein Belonging to a Novel Class of Biotechnologically Relevant Enzymes

scientific article

Structure-function correlation in glycine oxidase from Bacillus subtilis.

scientific article published on 22 April 2004

Structure-function relationships in human D-amino acid oxidase.

scientific article published on 03 August 2012

Structure-function relationships in human d-amino acid oxidase variants corresponding to known SNPs

scientific article

Studies on the reaction mechanism of Rhodotorula gracilis D-amino-acid oxidase. Role of the highly conserved Tyr-223 on substrate binding and catalysis

scientific article published on 01 December 1999

Substitution of Arginine 120 in Human D-Amino Acid Oxidase Favors FAD-Binding and Nuclear Mistargeting

scientific article published on 12 November 2019

The Gliotransmitter d-Serine Promotes Synapse Maturation and Axonal Stabilization In Vivo.

scientific article published on 26 May 2017

The degradation (by distinct pathways) of human D-amino acid oxidase and its interacting partner pLG72--two key proteins in D-serine catabolism in the brain.

scientific article published on 10 December 2013

The levels of the NMDA receptor co-agonist D-serine are reduced in the substantia nigra of MPTP-lesioned macaques and in the cerebrospinal fluid of Parkinson's disease patients

scientific article published on 20 June 2019

The primary structure of D-amino acid oxidase from Rhodotorula gracilis

article published in 1995

The x-ray structure of D-amino acid oxidase at very high resolution identifies the chemical mechanism of flavin-dependent substrate dehydrogenation

scientific article

Understanding renal nuclear protein accumulation: an in vitro approach to explain an in vivo phenomenon

scientific article

Unfolding intermediate in the peroxisomal flavoprotein D-amino acid oxidase.

scientific article published on 21 April 2004

Yeast D-amino acid oxidase: structural basis of its catalytic properties

scientific article

d-Serine diffusion through the blood–brain barrier: Effect on d-serine compartmentalization and storage ⬇️

scientific article published on March 23, 2012

pH and kinetic isotope effects in d-amino acid oxidase catalysis.

scientific article published in November 2001

pLG72 modulates intracellular D-serine levels through its interaction with D-amino acid oxidase: effect on schizophrenia susceptibility

scientific article

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