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List of works by Barbara Imperiali

A Modular Approach to Phosphoglycosyltransferase Inhibitors Inspired by Nucleoside Antibiotics.

scientific article

A Rapid and Efficient Luminescence-based Method for Assaying Phosphoglycosyltransferase Enzymes.

scientific article published on 14 September 2016

A Strategic Approach for Fluorescence Imaging of Membrane Proteins in a Native-like Environment

scientific article published on 09 December 2019

A chemist's approach to biochemical complexity. Interview by Susan Aldridge.

scientific article published in February 2003

A general screening strategy for peptide-based fluorogenic ligands: probes for dynamic studies of PDZ domain-mediated interactions

scientific article

A molecular basis for glycosylation-induced conformational switching ⬇️

scientific article published on August 1, 1998

A multiplexed homogeneous fluorescence-based assay for protein kinase activity in cell lysates

scientific article

A new environment-sensitive fluorescent amino acid for Fmoc-based solid phase peptide synthesis

scientific article published on 22 June 2004

A p38α-selective chemosensor for use in unfractionated cell lysates

scientific article published on 30 September 2010

A powerful combinatorial screen to identify high-affinity terbium(III)-binding peptides

scientific article

A rapid method for generation of selective Sox-based chemosensors of Ser/Thr kinases using combinatorial peptide libraries.

scientific article

A versatile amino acid analogue of the solvatochromic fluorophore 4-N,N-dimethylamino-1,8-naphthalimide: a powerful tool for the study of dynamic protein interactions

scientific article

Affinity-capture tandem mass spectrometric characterization of polyprenyl-linked oligosaccharides: tool to study protein N-glycosylation pathways.

scientific article published on 12 June 2008

Analysis of a dual domain phosphoglycosyl transferase reveals a ping-pong mechanism with a covalent enzyme intermediate.

scientific article

Analysis of the conserved glycosylation site in the nicotinic acetylcholine receptor: potential roles in complex assembly ⬇️

scientific article published on November 1, 1995

Antibodies from multiple sclerosis patients preferentially recognize hyperglucosylated adhesin of non-typeable Haemophilus influenzae.

scientific article published on 23 December 2016

Asparagine-linked protein glycosylation: from eukaryotic to prokaryotic systems

scientific article

At the membrane frontier: a prospectus on the remarkable evolutionary conservation of polyprenols and polyprenyl-phosphates.

scientific article published on 10 November 2011

Bacterial N-Glycosylation Efficiency Is Dependent on the Structural Context of Target Sequons.

scientific article published on 29 August 2016

Bacterial carbohydrate diversity - a Brave New World

scientific article published on 06 June 2019

Bacterial phosphoglycosyl transferases: initiators of glycan biosynthesis at the membrane interface

scientific article published in September 2017

Biochemical Analysis and Structure Determination of Bacterial Acetyltransferases Responsible for the Biosynthesis of UDP-N,N'-Diacetylbacillosamine

scientific article

Biochemical characterization of the O-linked glycosylation pathway in Neisseria gonorrhoeae responsible for biosynthesis of protein glycans containing N,N'-diacetylbacillosamine

scientific article

Biochemical evidence for an alternate pathway in N-linked glycoprotein biosynthesis

scientific article published on 28 April 2013

Biogenesis of Asparagine-Linked Glycoproteins Across Domains of Life-Similarities and Differences.

scientific article published on 26 February 2018

Biomimetic divalent ligands for the acute disruption of synaptic AMPAR stabilization

scientific article

Biosynthesis of UDP-GlcNAc(3NAc)A by WbpB, WbpE, and WbpD: enzymes in the Wbp pathway responsible for O-antigen assembly in Pseudomonas aeruginosa PAO1

scientific article

Biosynthesis of UDP-N,N'-diacetylbacillosamine in Acinetobacter baumannii: Biochemical characterization and correlation to existing pathways

scientific article published on 05 June 2013

Caged O-phosphorothioyl amino acids as building blocks for Fmoc-based solid phase peptide synthesis

scientific article published on July 2007

Caged mono- and divalent ligands for light-assisted disruption of PDZ domain-mediated interactions

scientific article published on 27 March 2013

Caged phospho-amino acid building blocks for solid-phase peptide synthesis

scientific article published in August 2003

Caged phosphopeptides reveal a temporal role for 14-3-3 in G1 arrest and S-phase checkpoint function.

scientific article published on 25 July 2004

Caged phosphoproteins

scientific article published in January 2005

Campylobacter jejuni PglH is a single active site processive polymerase that utilizes product inhibition to limit sequential glycosyl transfer reactions

scientific article

Chemical approaches for investigating phosphorylation in signal transduction networks.

scientific article

Chemical tools for studying directed cell migration.

scientific article published on 20 October 2011

Chemoenzymatic Assembly of Bacterial Glycoconjugates for Site-Specific Orthogonal Labeling.

scientific article

Chemoenzymatic Synthesis and Applications of Prokaryote-Specific UDP-Sugars.

scientific article published on 5 July 2017

Chemoenzymatic synthesis of glycopeptides with PglB, a bacterial oligosaccharyl transferase from Campylobacter jejuni

scientific journal article

Chemoenzymatic synthesis of polyprenyl phosphates.

scientific article published on 14 March 2008

Conformational dynamics and alignment properties of loop lanthanide-binding-tags (LBTs) studied in interleukin-1β.

scientific article

Conservation and Covariance in Small Bacterial Phosphoglycosyltransferases Identify the Functional Catalytic Core

scientific article published on 24 November 2015

Covalent Modification of Synthetic Hydrogels with Bioactive Proteins via Sortase-Mediated Ligation.

scientific article

Crystal Structure and Catalytic Mechanism of PglD from Campylobacter jejuni

scientific article

Deploying Fluorescent Nucleoside Analogues for High-Throughput Inhibitor Screening

scientific article published on 03 December 2019

Design Principles for SuCESsFul Biosensors: Specific Fluorophore/Analyte Binding and Minimization of Fluorophore/Scaffold Interactions

scientific article

Design of a heterospecific, tetrameric, 21-residue miniprotein with mixed alpha/beta structure.

scientific article

Design, solid-phase synthesis and evaluation of enterobactin analogs for iron delivery into the human pathogen Campylobacter jejuni

scientific article published on 16 April 2018

Design, synthesis, and characterization of caged compounds

scientific article published on 01 January 2007

Development of a fluorogenic sensor for activated Cdc42.

scientific article

Development of a multicomponent kinetic assay of the early enzymes in the Campylobacter jejuni N-linked glycosylation pathway.

scientific article published on 29 October 2010

Direct biochemical evidence for the utilization of UDP-bacillosamine by PglC, an essential glycosyl-1-phosphate transferase in the Campylobacter jejuni N-linked glycosylation pathway

scientific journal article

Double-Lanthanide-Binding Tags: Design, Photophysical Properties, and NMR Applications

article by Langdon J. Martin et al published June 2007 in Journal of the American Chemical Society

Double-lanthanide-binding tags for macromolecular crystallographic structure determination

scientific article

Dynamic and specific interaction between synaptic NR2-NMDA receptor and PDZ proteins

scientific article

Effects of glycosylation on peptide conformation: a synergistic experimental and computational study.

scientific article

Enantioselective synthesis and application of the highly fluorescent and environment-sensitive amino acid 6-(2-dimethylaminonaphthoyl) alanine (DANA).

scientific article published in September 2002

Encoded loop-lanthanide-binding tags for long-range distance measurements in proteins by NMR and EPR spectroscopy

scientific article published on 04 September 2015

Engineering encodable lanthanide-binding tags into loop regions of proteins

scientific article

Equilibrium and dynamic design principles for binding molecules engineered for reagentless biosensors.

scientific article published on 9 May 2014

Erratum: Antibodies from multiple sclerosis patients preferentially recognize hyperglucosylated adhesin of non-typeable Haemophilus influenzae

scientific article published on 22 March 2017

Exploiting topological constraints to reveal buried sequence motifs in the membrane-bound N-linked oligosaccharyl transferases

scientific article

Expression of N-terminal Cys-protein fragments using an intein refolding strategy.

scientific article

FRET-capture: a sensitive method for the detection of dynamic protein interactions

scientific article published on 13 December 2012

Facile Solid-Phase Synthesis and Assessment of Nucleoside Analogs as Inhibitors of Bacterial UDP-Sugar Processing Enzymes

article by Amaël G E Madec et al published 21 September 2018 in ACS Chemical Biology

Facile Solid-Phase Synthesis and Assessment of Nucleoside Analogs as Inhibitors of Bacterial UDP-Sugar Processing Enzymes

scholarly article by Amaël G. E. Madec et al published 6 August 2018 in ACS Chemical Biology

Fluorescent amino acids: modular building blocks for the assembly of new tools for chemical biology.

scientific article published on 22 April 2013

Fluorescent caged phosphoserine peptides as probes to investigate phosphorylation-dependent protein associations

scientific article published in August 2003

Fluorogenic probes for monitoring peptide binding to class II MHC proteins in living cells

scientific article

From peptide to protein: comparative analysis of the substrate specificity of N-linked glycosylation in C. jejuni.

scientific article

General method for the synthesis of caged phosphopeptides: tools for the exploration of signal transduction pathways

scientific article published on 01 August 2002

Genetic and molecular analyses reveal an evolutionary trajectory for glycan synthesis in a bacterial protein glycosylation system

scientific article

Heterologous expression and biophysical characterization of soluble oligosaccharyl transferase subunits

scientific article published in November 2004

Improving glycopeptide synthesis: a convenient protocol for the preparation of beta-glycosylamines and the synthesis of glycopeptides.

scientific article published in April 2005

In situ photoactivation of a caged phosphotyrosine peptide derived from focal adhesion kinase temporarily halts lamellar extension of single migrating tumor cells.

scientific article

In vitro assembly of the undecaprenylpyrophosphate-linked heptasaccharide for prokaryotic N-linked glycosylation

scientific article

In vitro biosynthesis of UDP-N,N'-diacetylbacillosamine by enzymes of the Campylobacter jejuni general protein glycosylation system

scientific journal article

In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis

scientific article

Insights into the key determinants of membrane protein topology enable the identification of new monotopic folds

scientific article published on 31 August 2018

Interrogating biology with a chemical lexicon ⬇️

scientific article published on 01 April 2009

Interrogating signaling nodes involved in cellular transformations using kinase activity probes

scientific article

Introduction of Caged Peptide/Protein into Cells Using Microinjection: Figure 1 ⬇️

scientific article published on 01 January 2007

Introduction of caged peptide/protein into cells using bead loading

scientific article published on 01 January 2007

Investigating bacterial N-linked glycosylation: synthesis and glycosyl acceptor activity of the undecaprenyl pyrophosphate-linked bacillosamine

scientific article published in October 2005

Investigation of the conserved reentrant membrane helix in the monotopic phosphoglycosyl transferase superfamily supports key molecular interactions with polyprenol phosphate substrates

scientific article published on 26 September 2019

Lanthanide-Binding Tags as Versatile Protein Coexpression Probes

scientific article published on 01 April 2003

Lanthanide-Binding Tags for 3D X-ray Imaging of Proteins in Cells at Nanoscale Resolution

scientific article published on 21 January 2020

Lanthanide-binding tags as luminescent probes for studying protein interactions

scientific article published on 01 June 2006

Lanthanide-binding tags with unnatural amino acids: sensitizing Tb3+ and Eu3+ luminescence at longer wavelengths.

scientific article

Lanthanide-tagged proteins--an illuminating partnership.

scientific article

Light-triggered myosin activation for probing dynamic cellular processes.

scientific article

Lipid bilayer nanodisc platform for investigating polyprenol-dependent enzyme interactions and activities

scientific article published on 03 December 2013

Membrane association of monotopic phosphoglycosyl transferase underpins function

scientific article published on 16 May 2018

Modular and tunable chemosensor scaffold for divalent zinc

scientific article published in September 2003

Modulation of Shank3 PDZ domain ligand-binding affinity by dimerization

scientific article published on 01 September 2010

Monitoring protein interactions and dynamics with solvatochromic fluorophores

scientific article

Monitoring protein kinases in cellular media with highly selective chimeric reporters.

scientific article

Monotopic Membrane Proteins Join the Fold

scientific article published on 15 October 2018

N-Linked Glycans Are Assembled on Highly Reduced Dolichol Phosphate Carriers in the Hyperthermophilic Archaea Pyrococcus furiosus

scientific article published on 22 June 2015

Neoglycopeptides as inhibitors of oligosaccharyl transferase: insight into negotiating product inhibition.

scientific article

New Synthetic Amino Acids for the Design and Synthesis of Peptide-Based Metal Ion Sensors

scientific article published in December 1996

Oligomeric beta(beta)(alpha) miniprotein motifs: pivotal role of single hinge residue in determining the oligomeric state

scientific article published in January 2002

Oligosaccharyl transferase: gatekeeper to the secretory pathway

scientific article (publication date: December 2002)

Optimal Sox-based fluorescent chemosensor design for serine/threonine protein kinases.

scientific article published on 20 March 2006

Optimized protocol for expression and purification of membrane-bound PglB, a bacterial oligosaccharyl transferase.

scientific article published on 12 April 2013

Peptides to peptidomimetics: towards the design and synthesis of bioavailable inhibitors of oligosaccharyl transferase

scientific article published in January 2003

Peptidyl chemosensors incorporating a FRET mechanism for detection of Ni(II).

scientific article

Perturbing the folding energy landscape of the bacterial immunity protein Im7 by site-specific N-linked glycosylation

scientific article

Photolytic control of peptide self-assembly

scientific article

Photophysics and biological applications of the environment-sensitive fluorophore 6-N,N-dimethylamino-2,3-naphthalimide.

scientific article published in February 2005

Polyisoprenol specificity in the Campylobacter jejuni N-linked glycosylation pathway

scientific article

Preface

Preface

Probing Polytopic Membrane Protein-Substrate Interactions by Luminescence Resonance Energy Transfer

scientific article

Protein Alignment by a Coexpressed Lanthanide-Binding Tag for the Measurement of Residual Dipolar Couplings

scientific article published on 01 November 2003

Protein oligomerization: how and why.

scientific article

Quantification of protein kinase enzymatic activity in unfractionated cell lysates using CSox-based sensors

scientific article

Recognition-domain focused chemosensors: versatile and efficient reporters of protein kinase activity.

scientific article published on 29 August 2008

Selective biochemical labeling of Campylobacter jejuni cell-surface glycoconjugates.

scientific article published on 11 March 2015

Selective mitogen activated protein kinase activity sensors through the application of directionally programmable D domain motifs

scientific article

Semisynthesis of a glycosylated Im7 analogue for protein folding studies

scientific article published on September 2005

Semisynthesis of unnatural amino acid mutants of paxillin: protein probes for cell migration studies.

scientific article published on 22 January 2007

Sequential activation and deactivation of protein function using spectrally differentiated caged phosphoamino acids.

scientific article

Solution Structure of Alg13: The Sugar Donor Subunit of a Yeast N-Acetylglucosamine Transferase

scientific article

Stereochemical Divergence of Polyprenol Phosphate Glycosyltransferases.

scientific article published on 25 November 2017

Structural Analysis of WbpE from Pseudomonas aeruginosa PAO1: A Nucleotide Sugar Aminotransferase Involved in O-Antigen Assembly,

scientific article

Structural Origin of the High Affinity of a Chemically Evolved Lanthanide-Binding Peptide

scientific article published on 01 July 2004

Structural and Functional Analysis of Peptidyl Oligosaccharyl Transferase Inhibitors ⬇️

scientific article published on October 14, 1997

Structural and mechanistic themes in glycoconjugate biosynthesis at membrane interfaces

scientific article published on 16 April 2019

Structure determination of a Galectin-3-carbohydrate complex using paramagnetism-based NMR constraints

scientific article published on 15 April 2008

Study of the stability and unfolding mechanism of BBA1 by molecular dynamics simulations at different temperatures.

scientific article published on June 1999

Synthesis of anhydride precursors of the environment-sensitive fluorophores 4-DMAP and 6-DMN.

scientific article

Synthesis of red-shifted 8-hydroxyquinoline derivatives using click chemistry and their incorporation into phosphorylation chemosensors.

scientific article published on October 2009

Tailoring chimeric ligands for studying and biasing ErbB receptor family interactions

scientific article

Tailoring encodable lanthanide-binding tags as MRI contrast agents

scientific article

Targeting Bacillosamine Biosynthesis in Bacterial Pathogens: Development of Inhibitors to a Bacterial Amino-Sugar Acetyltransferase from Campylobacter jejuni

scientific article published on 9 February 2017

The Chemistry–Glycobiology Frontier ⬇️

scientific article published on October 31, 2012

The best and the brightest: exploiting tryptophan-sensitized Tb(3+) luminescence to engineer lanthanide-binding tags.

scientific article published on January 2015

The engineering of membrane-permeable peptides.

scientific article published in June 2005

The expanding horizons of asparagine-linked glycosylation

scientific article

The interplay of glycosylation and disulfide formation influences fibrillization in a prion protein fragment

scientific article

The renaissance of bacillosamine and its derivatives: pathway characterization and implications in pathogenicity

scientific article published on 21 January 2014

Thiol-reactive derivatives of the solvatochromic 4-N,N-dimethylamino-1,8-naphthalimide fluorophore: a highly sensitive toolset for the detection of biomolecular interactions

scientific article published on November 2009

Tools for investigating peptide-protein interactions: peptide incorporation of environment-sensitive fluorophores through SPPS-based 'building block' approach.

scientific article

Tools for investigating peptide-protein interactions: peptide incorporation of environment-sensitive fluorophores via on-resin derivatization.

scientific article

Two-photon fluorescence spectroscopy and imaging of 4-dimethylaminonaphthalimide peptide and protein conjugates

scientific article published on 27 November 2013

Versatile fluorescence probes of protein kinase activity.

scientific article published in November 2003

X-ray structure analysis of a designed oligomeric miniprotein reveals a discrete quaternary architecture.

scholarly article

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