List of works - Chrisostomos Prodromou

4,5-diarylisoxazole Hsp90 chaperone inhibitors: potential therapeutic agents for the treatment of cancer

scientific article

A combinatorial method to enable detailed investigation of protein-protein interactions

scientific article published on March 2011

A common conformationally coupled ATPase mechanism for yeast and human cytoplasmic HSP90s

scientific article published on 20 November 2008

A molecular clamp in the crystal structure of the N-terminal domain of the yeast Hsp90 chaperone

scientific article

A simple yeast-based system for analyzing inhibitor resistance in the human cancer drug targets Hsp90alpha/beta

scientific article published on 4 February 2010

A two-hybrid screen of the yeast proteome for Hsp90 interactors uncovers a novel Hsp90 chaperone requirement in the activity of a stress-activated mitogen-activated protein kinase, Slt2p (Mpk1p).

scientific article

ATP binding and hydrolysis are essential to the function of the Hsp90 molecular chaperone in vivo

scientific article

Activation of the ATPase activity of hsp90 by the stress-regulated cochaperone aha1

scientific article

Advances on the Structure of the R2TP/Prefoldin-like Complex

scientific article published on 01 January 2018

Assays for HSP90 and inhibitors.

scientific article

Asymmetric Hsp90 N domain SUMOylation recruits Aha1 and ATP-competitive inhibitors

scientific article published on January 2014

Author Correction: RPAP3 provides a flexible scaffold for coupling HSP90 to the human R2TP co-chaperone complex

scientific article published in Nature Communications

Backbone resonance assignments of the 25kD N-terminal ATPase domain from the Hsp90 chaperone

article

CDK-dependent Hsp70 Phosphorylation controls G1 cyclin abundance and cell-cycle progression

scientific article

Chaperone ligand-discrimination by the TPR-domain protein Tah1.

scientific article

Chaperoned ubiquitylation--crystal structures of the CHIP U box E3 ubiquitin ligase and a CHIP-Ubc13-Uev1a complex

scientific article

Charged linker sequence modulates eukaryotic heat shock protein 90 (Hsp90) chaperone activity

scientific article published on 6 February 2012

Chemical Perturbation of Oncogenic Protein Folding: from the Prediction of Locally Unstable Structures to the Design of Disruptors of Hsp90-Client Interactions

scientific article published on 13 March 2020

Clinical and functional analyses of AIPL1 variants reveal mechanisms of pathogenicity linked to different forms of retinal degeneration

scientific article published on 16 October 2020

Cloning of the HIS3 gene of Yarrowia lipolytica

scientific article

Co-Crystalization and In Vitro Biological Characterization of 5-Aryl-4-(5-Substituted-2-4-Dihydroxyphenyl)-1,2,3-Thiadiazole Hsp90 Inhibitors

scientific article

Co-chaperone regulation of conformational switching in the Hsp90 ATPase cycle

scientific article published on 2 October 2004

Combinatorial Domain Hunting: An effective approach for the identification of soluble protein domains adaptable to high-throughput applications

scientific article published in October 2006

Cooperation of local motions in the Hsp90 molecular chaperone ATPase mechanism.

scientific article published on 20 June 2016

Correction: In vitro Biological Characterization of a Novel, Synthetic Diaryl Pyrazole Resorcinol Class of Heat Shock Protein 90 Inhibitors

scientific article published on 01 January 2019

Crystal structure of an Hsp90-nucleotide-p23/Sba1 closed chaperone complex

scientific article (publication date: 20 April 2006)

DNA fragmentation based combinatorial approaches to soluble protein expression Part II: library expression, screening and scale-up

scientific article

DNA fragmentation-based combinatorial approaches to soluble protein expression Part I. Generating DNA fragment libraries

scientific article

Detection of the ATPase activity of the molecular chaperones Hsp90 and Hsp72 using the TranscreenerTM ADP assay kit.

scientific article published on 10 February 2010

Differential Regulation of G1 CDK Complexes by the Hsp90-Cdc37 Chaperone System.

scientific article published in October 2017

Dihydropyridine Derivatives Modulate Heat Shock Responses and have a Neuroprotective Effect in a Transgenic Mouse Model of Alzheimer's Disease

scientific article

Dihydropyridines Allosterically Modulate Hsp90 Providing a Novel Mechanism for Heat Shock Protein Co-induction and Neuroprotection.

scientific article

Dynamic tyrosine phosphorylation modulates cycling of the HSP90-P50(CDC37)-AHA1 chaperone machine

scientific article

Editorial: The Role of Heat Shock Proteins in Neuroprotection

scientific article published on 06 August 2020

Erratum: Corrigendum: ATP-competitive inhibitors block protein kinase recruitment to the Hsp90-Cdc37 system

scholarly article published in Nature Chemical Biology

Expressed in the yeast Saccharomyces cerevisiae, human ERK5 is a client of the Hsp90 chaperone that complements loss of the Slt2p (Mpk1p) cell integrity stress-activated protein kinase

scientific article published on September 2006

Expression and crystallization of the yeast Hsp82 chaperone, and preliminary x-ray diffraction studies of the amino-terminal domain

scientific article published on 01 August 1996

Expression and crystallization of the yeast Hsp82 chaperone, and preliminary x‐ray diffraction studies of the amino‐terminal domain

scholarly article by Chrisostomos Prodromou published in August 1996

Features of the Streptomyces hygroscopicus HtpG reveal how partial geldanamycin resistance can arise with mutation to the ATP binding pocket of a eukaryotic Hsp90

scientific article

HECTD3 Mediates an HSP90-Dependent Degradation Pathway for Protein Kinase Clients.

scientific article published on June 2017

High-throughput screening assay for inhibitors of heat-shock protein 90 ATPase activity

scientific article published in April 2004

Hsp90 charged-linker truncation reverses the functional consequences of weakened hydrophobic contacts in the N domain

scientific article published on 18 October 2009

Hsp90 middle domain phosphorylation initiates a complex conformational program to recruit the ATPase-stimulating cochaperone Aha1.

scientific article published on 12 June 2019

Hsp90-dependent activation of protein kinases is regulated by chaperone-targeted dephosphorylation of Cdc37.

scientific article published on September 2008

Identification and structural characterization of the ATP/ADP-binding site in the Hsp90 molecular chaperone

scientific article

In the yeast heat shock response, Hsf1-directed induction of Hsp90 facilitates the activation of the Slt2 (Mpk1) mitogen-activated protein kinase required for cell integrity

scientific article published on 9 February 2007

In vitro Biological Characterization of a Novel, Synthetic Diaryl Pyrazole Resorcinol Class of Heat Shock Protein 90 Inhibitors

In vivo bioassay to test the pathogenicity of missense human AIP variants

scientific article published on 9 April 2018

Inhibition of Hsp90 with resorcylic acid macrolactones: synthesis and binding studies

scientific article

Inhibition of Hsp90 with synthetic macrolactones: synthesis and structural and biological evaluation of ring and conformational analogs of radicicol

scientific article published on November 2006

Inhibition of the heat shock protein 90 molecular chaperone in vitro and in vivo by novel, synthetic, potent resorcinylic pyrazole/isoxazole amide analogues

scientific article

Investigating the protein-protein interactions of the yeast Hsp90 chaperone system by two-hybrid analysis: potential uses and limitations of this approach

scientific article published on January 2004

Mechanisms of Hsp90 regulation

scientific article

Molecular characterization of macbecin as an Hsp90 inhibitor

scientific article

Mps1 Mediated Phosphorylation of Hsp90 Confers Renal Cell Carcinoma Sensitivity and Selectivity to Hsp90 Inhibitors

scientific article published on 19 January 2016

Multi-chaperone function modulation and association with cytoskeletal proteins are key features of the function of AIP in the pituitary gland

scientific article published on 11 January 2018

NVP-AUY922: a novel heat shock protein 90 inhibitor active against xenograft tumor growth, angiogenesis, and metastasis

scientific article

Optimizing natural products by biosynthetic engineering: discovery of nonquinone Hsp90 inhibitors

scientific article

Post-translational Regulation of FNIP1 Creates a Rheostat for the Molecular Chaperone Hsp90

scientific article published on 01 January 2019

Posttranslational modification and conformational state of heat shock protein 90 differentially affect binding of chemically diverse small molecule inhibitors

scientific article

Qri2/Nse4, a component of the essential Smc5/6 DNA repair complex

scientific article

RPAP3 provides a flexible scaffold for coupling HSP90 to the human R2TP co-chaperone complex

scientific article published on 16 April 2018

Rapid Proteasomal Degradation of Mutant Proteins Is the Primary Mechanism Leading to Tumorigenesis in Patients With Missense AIP Mutations.

scientific article published on 02 June 2016

Recursive PCR: a novel technique for total gene synthesis

scientific article published on December 1, 1992

Regulation of Hsp90 ATPase activity by tetratricopeptide repeat (TPR)-domain co-chaperones

scientific article

Regulation of Hsp90 ATPase activity by the co-chaperone Cdc37p/p50cdc37.

scientific article

Regulatory Mechanisms of Hsp90.

scientific article published on January 2017

Restricting direct interaction of CDC37 with HSP90 does not compromise chaperoning of client proteins

scientific article published on 2 December 2013

Sensitivity to Hsp90-targeting drugs can arise with mutation to the Hsp90 chaperone, cochaperones and plasma membrane ATP binding cassette transporters of yeast

scientific article

Strategies for stalling malignancy: targeting cancer's addiction to Hsp90.

scientific article published on January 2009

Structural Basis for Assembly of Hsp90-Sgt1-CHORD Protein Complexes: Implications for Chaperoning of NLR Innate Immunity Receptors

scientific article

Structural Basis for Phosphorylation-Dependent Recruitment of Tel2 to Hsp90 by Pih1

scientific article

Structural and functional analysis of the middle segment of hsp90: implications for ATP hydrolysis and client protein and cochaperone interactions

scientific article (publication date: March 2003)

Structural and functional coupling of Hsp90- and Sgt1-centred multi-protein complexes

scientific article

Structural basis for inhibition of the Hsp90 molecular chaperone by the antitumor antibiotics radicicol and geldanamycin

scientific article

Structural basis for recruitment of the ATPase activator Aha1 to the Hsp90 chaperone machinery

scientific article

Structural basis for recruitment of the ATPase activator Aha1 to the Hsp90 chaperone machinery.

scientific article published in March 2004

Structural basis of the radicicol resistance displayed by a fungal hsp90

scientific article

Structural mechanism for regulation of the AAA-ATPases RUVBL1-RUVBL2 in the R2TP co-chaperone revealed by cryo-EM

scientific article published on 01 May 2019

Structural-thermodynamic relationships of interactions in the N-terminal ATP-binding domain of Hsp90.

scientific article published on 21 July 2009

Structure and functional relationships of Hsp90.

scientific article

Structure and in vivo function of Hsp90.

scientific article

Structure of an Hsp90-Cdc37-Cdk4 complex.

scientific article published in September 2006

Structure of the TELO2-TTI1-TTI2 complex and its function in TOR recruitment to the R2TP chaperone

scientific article

Structure of the TPR Domain of AIP: Lack of Client Protein Interaction with the C-Terminal α-7 Helix of the TPR Domain of AIP Is Sufficient for Pituitary Adenoma Predisposition

scientific article published in 2012

Structure, function, and mechanism of the Hsp90 molecular chaperone

scientific article

Swe1Wee1-dependent tyrosine phosphorylation of Hsp90 regulates distinct facets of chaperone function

scientific article

Synthesis of 19-substituted geldanamycins with altered conformations and their binding to heat shock protein Hsp90

scientific article

Synthesis of a modified gene encoding human ornithine transcarbamylase for expression in mammalian mitochondrial and universal translation systems: a novel approach towards correction of a genetic defect

scientific article published in March 1996

Synthesis of macrolactam analogues of radicicol and their binding to heat shock protein Hsp90

scientific article

Tah1 helix-swap dimerization prevents mixed Hsp90 co-chaperone complexes

scientific article published on 25 April 2015

Targeting the Hsp90 molecular chaperone with novel macrolactams. Synthesis, structural, binding, and cellular studies

scientific article

The ATPase cycle of Hsp90 drives a molecular 'clamp' via transient dimerization of the N-terminal domains

scientific article

The ATPase-dependent chaperoning activity of Hsp90a regulates thick filament formation and integration during skeletal muscle myofibrillogenesis

scientific article (publication date: March 2008)

The FNIP co-chaperones decelerate the Hsp90 chaperone cycle and enhance drug binding

scientific journal article

The Hsp90 molecular chaperone: an open and shut case for treatment

scientific article

The Hsp90 mosaic: a picture emerges

scientific article published in January 2009

The Hsp90 of Candida albicans can confer Hsp90 functions in Saccharomyces cerevisiae: a potential model for the processes that generate immunogenic fragments of this molecular chaperone in C. albicans infections

article

The Mechanism of Hsp90 regulation by the protein kinase-specific cochaperone p50(cdc37)

scientific article

The Stoichiometric Interaction of the Hsp90-Sgt1-Rar1 Complex by CD and SRCD Spectroscopy

scientific article published in January 2017

The Structure of the R2TP Complex Defines a Platform for Recruiting Diverse Client Proteins to the HSP90 Molecular Chaperone System

scientific article

The aconitase of Escherichia coli. Nucleotide sequence of the aconitase gene and amino acid sequence similarity with mitochondrial aconitases, the iron-responsive-element-binding protein and isopropylmalate isomerases

scientific article published on March 1, 1992

The aconitase of Escherichia coli: purification of the enzyme and molecular cloning and map location of the gene (acn)

scientific article published on November 1, 1991

The identification, synthesis, protein crystal structure and in vitro biochemical evaluation of a new 3,4-diarylpyrazole class of Hsp90 inhibitors

scientific article published on July 2005

The integrity and organization of the human AIPL1 functional domains is critical for its role as a HSP90-dependent co-chaperone for rod PDE6.

scientific article published on 26 August 2017

The integrity and organization of the human AIPL1 functional domains is critical for its role as a HSP90-dependent co-chaperone for rod PDE6.

scientific article published on 16 January 2018

The structure of FKBP38 in complex with the MEEVD tetratricopeptide binding-motif of Hsp90

scientific article

The structure-function relationship of oncogenic LMTK3

scientific article published on 13 November 2020

The ‘active life’ of Hsp90 complexes ⬇️

scientific article published on August 4, 2011

Threonine 22 phosphorylation attenuates Hsp90 interaction with cochaperones and affects its chaperone activity

scientific article

Tumor suppressor Tsc1 is a new Hsp90 co-chaperone that facilitates folding of kinase and non-kinase clients.

scientific article published on 10 November 2017

Yeast is selectively hypersensitised to heat shock protein 90 (Hsp90)-targetting drugs with heterologous expression of the human Hsp90beta, a property that can be exploited in screens for new Hsp90 chaperone inhibitors

scientific article published in January 2003

c-Abl Mediated Tyrosine Phosphorylation of Aha1 Activates Its Co-chaperone Function in Cancer Cells

scientific article published on 28 July 2015

List of works by Chrisostomos Prodromou

4,5-diarylisoxazole Hsp90 chaperone inhibitors: potential therapeutic agents for the treatment of cancer

A combinatorial method to enable detailed investigation of protein-protein interactions

A common conformationally coupled ATPase mechanism for yeast and human cytoplasmic HSP90s

A molecular clamp in the crystal structure of the N-terminal domain of the yeast Hsp90 chaperone

A simple yeast-based system for analyzing inhibitor resistance in the human cancer drug targets Hsp90alpha/beta

A two-hybrid screen of the yeast proteome for Hsp90 interactors uncovers a novel Hsp90 chaperone requirement in the activity of a stress-activated mitogen-activated protein kinase, Slt2p (Mpk1p).

ATP binding and hydrolysis are essential to the function of the Hsp90 molecular chaperone in vivo

Activation of the ATPase activity of hsp90 by the stress-regulated cochaperone aha1

Advances on the Structure of the R2TP/Prefoldin-like Complex

Assays for HSP90 and inhibitors.

Asymmetric Hsp90 N domain SUMOylation recruits Aha1 and ATP-competitive inhibitors

Author Correction: RPAP3 provides a flexible scaffold for coupling HSP90 to the human R2TP co-chaperone complex

Backbone resonance assignments of the 25kD N-terminal ATPase domain from the Hsp90 chaperone

CDK-dependent Hsp70 Phosphorylation controls G1 cyclin abundance and cell-cycle progression

Chaperone ligand-discrimination by the TPR-domain protein Tah1.

Chaperoned ubiquitylation--crystal structures of the CHIP U box E3 ubiquitin ligase and a CHIP-Ubc13-Uev1a complex

Charged linker sequence modulates eukaryotic heat shock protein 90 (Hsp90) chaperone activity

Chemical Perturbation of Oncogenic Protein Folding: from the Prediction of Locally Unstable Structures to the Design of Disruptors of Hsp90-Client Interactions

Clinical and functional analyses of AIPL1 variants reveal mechanisms of pathogenicity linked to different forms of retinal degeneration

Cloning of the HIS3 gene of Yarrowia lipolytica

Co-Crystalization and In Vitro Biological Characterization of 5-Aryl-4-(5-Substituted-2-4-Dihydroxyphenyl)-1,2,3-Thiadiazole Hsp90 Inhibitors

Co-chaperone regulation of conformational switching in the Hsp90 ATPase cycle

Combinatorial Domain Hunting: An effective approach for the identification of soluble protein domains adaptable to high-throughput applications

Cooperation of local motions in the Hsp90 molecular chaperone ATPase mechanism.

Correction: In vitro Biological Characterization of a Novel, Synthetic Diaryl Pyrazole Resorcinol Class of Heat Shock Protein 90 Inhibitors

Crystal structure of an Hsp90-nucleotide-p23/Sba1 closed chaperone complex

DNA fragmentation based combinatorial approaches to soluble protein expression Part II: library expression, screening and scale-up

DNA fragmentation-based combinatorial approaches to soluble protein expression Part I. Generating DNA fragment libraries

Detection of the ATPase activity of the molecular chaperones Hsp90 and Hsp72 using the TranscreenerTM ADP assay kit.

Differential Regulation of G1 CDK Complexes by the Hsp90-Cdc37 Chaperone System.

Dihydropyridine Derivatives Modulate Heat Shock Responses and have a Neuroprotective Effect in a Transgenic Mouse Model of Alzheimer's Disease

Dihydropyridines Allosterically Modulate Hsp90 Providing a Novel Mechanism for Heat Shock Protein Co-induction and Neuroprotection.

Dynamic tyrosine phosphorylation modulates cycling of the HSP90-P50(CDC37)-AHA1 chaperone machine

Editorial: The Role of Heat Shock Proteins in Neuroprotection

Erratum: Corrigendum: ATP-competitive inhibitors block protein kinase recruitment to the Hsp90-Cdc37 system

Expressed in the yeast Saccharomyces cerevisiae, human ERK5 is a client of the Hsp90 chaperone that complements loss of the Slt2p (Mpk1p) cell integrity stress-activated protein kinase

Expression and crystallization of the yeast Hsp82 chaperone, and preliminary x-ray diffraction studies of the amino-terminal domain

Expression and crystallization of the yeast Hsp82 chaperone, and preliminary x‐ray diffraction studies of the amino‐terminal domain

Features of the Streptomyces hygroscopicus HtpG reveal how partial geldanamycin resistance can arise with mutation to the ATP binding pocket of a eukaryotic Hsp90

HECTD3 Mediates an HSP90-Dependent Degradation Pathway for Protein Kinase Clients.

High-throughput screening assay for inhibitors of heat-shock protein 90 ATPase activity

Hsp90 charged-linker truncation reverses the functional consequences of weakened hydrophobic contacts in the N domain

Hsp90 middle domain phosphorylation initiates a complex conformational program to recruit the ATPase-stimulating cochaperone Aha1.

Hsp90-dependent activation of protein kinases is regulated by chaperone-targeted dephosphorylation of Cdc37.

Identification and structural characterization of the ATP/ADP-binding site in the Hsp90 molecular chaperone

In the yeast heat shock response, Hsf1-directed induction of Hsp90 facilitates the activation of the Slt2 (Mpk1) mitogen-activated protein kinase required for cell integrity

In vitro Biological Characterization of a Novel, Synthetic Diaryl Pyrazole Resorcinol Class of Heat Shock Protein 90 Inhibitors

In vivo bioassay to test the pathogenicity of missense human AIP variants

Inhibition of Hsp90 with resorcylic acid macrolactones: synthesis and binding studies

Inhibition of Hsp90 with synthetic macrolactones: synthesis and structural and biological evaluation of ring and conformational analogs of radicicol

Inhibition of the heat shock protein 90 molecular chaperone in vitro and in vivo by novel, synthetic, potent resorcinylic pyrazole/isoxazole amide analogues

Investigating the protein-protein interactions of the yeast Hsp90 chaperone system by two-hybrid analysis: potential uses and limitations of this approach

Mechanisms of Hsp90 regulation

Molecular characterization of macbecin as an Hsp90 inhibitor

Mps1 Mediated Phosphorylation of Hsp90 Confers Renal Cell Carcinoma Sensitivity and Selectivity to Hsp90 Inhibitors

Multi-chaperone function modulation and association with cytoskeletal proteins are key features of the function of AIP in the pituitary gland

NVP-AUY922: a novel heat shock protein 90 inhibitor active against xenograft tumor growth, angiogenesis, and metastasis

Optimizing natural products by biosynthetic engineering: discovery of nonquinone Hsp90 inhibitors

Post-translational Regulation of FNIP1 Creates a Rheostat for the Molecular Chaperone Hsp90

Posttranslational modification and conformational state of heat shock protein 90 differentially affect binding of chemically diverse small molecule inhibitors

Qri2/Nse4, a component of the essential Smc5/6 DNA repair complex

RPAP3 provides a flexible scaffold for coupling HSP90 to the human R2TP co-chaperone complex

Rapid Proteasomal Degradation of Mutant Proteins Is the Primary Mechanism Leading to Tumorigenesis in Patients With Missense AIP Mutations.

Recursive PCR: a novel technique for total gene synthesis

Regulation of Hsp90 ATPase activity by tetratricopeptide repeat (TPR)-domain co-chaperones

Regulation of Hsp90 ATPase activity by the co-chaperone Cdc37p/p50cdc37.

Regulatory Mechanisms of Hsp90.

Restricting direct interaction of CDC37 with HSP90 does not compromise chaperoning of client proteins

Sensitivity to Hsp90-targeting drugs can arise with mutation to the Hsp90 chaperone, cochaperones and plasma membrane ATP binding cassette transporters of yeast

Strategies for stalling malignancy: targeting cancer's addiction to Hsp90.

Structural Basis for Assembly of Hsp90-Sgt1-CHORD Protein Complexes: Implications for Chaperoning of NLR Innate Immunity Receptors

Structural Basis for Phosphorylation-Dependent Recruitment of Tel2 to Hsp90 by Pih1

Structural and functional analysis of the middle segment of hsp90: implications for ATP hydrolysis and client protein and cochaperone interactions

Structural and functional coupling of Hsp90- and Sgt1-centred multi-protein complexes

Structural basis for inhibition of the Hsp90 molecular chaperone by the antitumor antibiotics radicicol and geldanamycin

Structural basis for recruitment of the ATPase activator Aha1 to the Hsp90 chaperone machinery

Structural basis for recruitment of the ATPase activator Aha1 to the Hsp90 chaperone machinery.

Structural basis of the radicicol resistance displayed by a fungal hsp90

Structural mechanism for regulation of the AAA-ATPases RUVBL1-RUVBL2 in the R2TP co-chaperone revealed by cryo-EM