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List of works by James C Whisstock

A Molecular Switch Governs the Interaction between the Human Complement Protease C1s and Its Substrate, Complement C4

scientific article

A common fold mediates vertebrate defense and bacterial attack

scientific article

A new model for pore formation by cholesterol-dependent cytolysins

scientific article (publication date: August 2014)

A serpin in the cellulosome of the anaerobic fungus Piromyces sp. strain E2.

scientific article

A structural basis for loop C-sheet polymerization in serpins

scientific article

A structural basis for the selection of dominant alphabeta T cell receptors in antiviral immunity

scientific article

A tale of two specificities: bispecific antibodies for therapeutic and diagnostic applications

scientific article published on 02 October 2013

A versatile monoclonal antibody specific to human SERPINB5

scientific article

AB5 subtilase cytotoxin inactivates the endoplasmic reticulum chaperone BiP.

scientific article

Aeropin from the extremophile Pyrobaculum aerophilum bypasses the serpin misfolding trap

scientific article

An Essential Role of Maspin in Embryogenesis and Tumor Suppression-Letter.

scientific article published on 6 September 2017

An atlas of serpin conformations.

scientific article published on February 1998

An overview of the serpin superfamily

scientific article

An ultrastructural investigation of tumors undergoing regression mediated by immunotherapy

scientific article published on 14 December 2017

Analysis of Perforin Assembly by Quartz Crystal Microbalance Reveals a Role for Cholesterol and Calcium-independent Membrane Binding

scientific article

Ancient but Not Forgotten: New Insights Into MPEG1, a Macrophage Perforin-Like Immune Effector

scientific article published on 15 October 2020

Antibodies: From novel repertoires to defining and refining the structure of biologically important targets.

scientific article

Antithrombin: in control of coagulation

scientific article published on March 2004

Assembling the machinery of coagulation

scientific article published on 01 October 2013

Automatic generation of protein structure cartoons with Pro-origami ⬇️

scientific article published on October 12, 2011

Bestatin-based chemical biology strategy reveals distinct roles for malaria M1- and M17-family aminopeptidases

scientific article

Bioinformatic approaches for predicting substrates of proteases

scientific article published on February 2011

Bone morphogenetic protein/retinoic acid inducible neural-specific protein (brinp) expression during Danio rerio development

scientific article

Brinp1(-/-) mice exhibit autism-like behaviour, altered memory, hyperactivity and increased parvalbumin-positive cortical interneuron density

scientific article

CLIMS: Crystallography Laboratory Information Management System

scientific article published on 26 August 2004

COOH-Terminal Clustering of Autoantibody and T-Cell Determinants on the Structure of GAD65 Provide Insights Into the Molecular Basis of Autoreactivity

scientific article published on 09 January 2008

Capturing embryonic development from metamorphosis: how did the terminal patterning signalling pathway of Drosophila evolve?

article

Cascleave 2.0, a new approach for predicting caspase and granzyme cleavage targets

scientific article

Cascleave: towards more accurate prediction of caspase substrate cleavage sites

scientific article published on 3 February 2010

Cationic sites on granzyme B contribute to cytotoxicity by promoting its uptake into target cells

scientific article

Cell Traversal Activity Is Important for Plasmodium falciparum Liver Infection in Humanized Mice

scientific article (publication date: 28 March 2017)

Characterization of an adapter subunit to a phosphatidylinositol (3)P 3-phosphatase: identification of a myotubularin-related protein lacking catalytic activity

scientific article

Characterization of epitope regions of thyrotropin β-subunit recognized by the monoclonal antibodies mAb279 and mAb299: a chimeric peptide approach

scientific article published on 01 September 1999

Characterization of the specificity of arginine-specific gingipains from Porphyromonas gingivalis reveals active site differences between different forms of the enzymes

scientific article published in October 2003

Cleaved antitrypsin polymers at atomic resolution

scientific article

Cloning and expression of the major secreted cathepsin B-like protein from juvenile Fasciola hepatica and analysis of immunogenicity following liver fluke infection.

scientific article

Computational analysis of evolution and conservation in a protein superfamily

scientific article

Conformational change in the chromatin remodelling protein MENT

scientific article

Conformational changes during pore formation by the perforin-related protein pleurotolysin

scientific article (publication date: February 2015)

Conformational changes in serpins: I. the native and cleaved conformations of α 1 -antitrypsin 1 1Edited by J. M. Thornton

Conformational changes in serpins: II. The mechanism of activation of antithrombin by heparin.

scientific article

Conservation within the myosin motor domain: implications for structure and function

article

Corrigendum: N-terminal domain of Bothrops asper Myotoxin II Enhances the Activity of Endothelin Converting Enzyme-1 and Neprilysin.

scientific article published on 22 April 2016

Crystal structure of TcpK in complex with oriT DNA of the antibiotic resistance plasmid pCW3

scientific article published in Nature Communications

Crystallization of the virulent and benign subtilisin-like proteases from the ovine footrot pathogen Dichelobacter nodosus

scientific article

Crystallography of serpins and serpin complexes

scientific article published in January 2011

DNA accelerates the inhibition of human cathepsin V by serpins

scientific article

Defining the interaction of perforin with calcium and the phospholipid membrane

scientific article

Determination of the P1', P2' and P3' subsite-specificity of factor Xa.

scientific article

Development of the Cellular Immune System of Drosophila Requires the Membrane Attack Complex/Perforin-Like Protein Torso-Like

scientific article

EGM: encapsulated gene-by-gene matching to identify gene orthologs and homologous segments in genomes

scientific article published on 27 June 2010

Efficient large-scale protein sequence comparison and gene matching to identify orthologs and co-orthologs ⬇️

scientific article published on December 30, 2011

Elucidation of the substrate specificity of the C1s protease of the classical complement pathway

scientific article published on 16 September 2005

Elucidation of the substrate specificity of the MASP-2 protease of the lectin complement pathway and identification of the enzyme as a major physiological target of the serpin, C1-inhibitor

scientific article

Evidence that serpin architecture intrinsically supports papain-like cysteine protease inhibition: engineering alpha(1)-antitrypsin to inhibit cathepsin proteases

scientific article

Federated repositories of X-ray diffraction images

scientific article

Fingerprinting the substrate specificity of M1 and M17 aminopeptidases of human malaria, Plasmodium falciparum

scientific article

Functional analysis of the Listeria monocytogenes secretion chaperone PrsA2 and its multiple contributions to bacterial virulence

scientific article

Functional insights from the distribution and role of homopeptide repeat-containing proteins

scientific article

GABA production by glutamic acid decarboxylase is regulated by a dynamic catalytic loop

scientific article

Genome-Wide Screen for New Components of the Drosophila melanogaster Torso Receptor Tyrosine Kinase Pathway

scientific article published on 23 January 2018

Giant MACPF/CDC pore forming toxins: A class of their own.

scientific article

GlycoMine: a machine learning-based approach for predicting N-, C- and O-linked glycosylation in the human proteome

scientific article published on 6 January 2015

Glycoprotein Ib-IX-V.

scientific article

High resolution structure of cleaved Serpin 42 Da from Drosophila melanogaster

scientific article

Highlight: The Biology of Proteolytic Systems

article

Hijacking of a Substrate-binding Protein Scaffold for use in Mycobacterial Cell Wall Biosynthesis

scientific article published on 25 April 2006

Homodimerization attenuates the anti-inflammatory activity of interleukin-37

scientific article published on 01 February 2017

Human clade B serpins (ov-serpins) belong to a cohort of evolutionarily dispersed intracellular proteinase inhibitor clades that protect cells from promiscuous proteolysis.

scientific article published on February 2004

Hurpin is a selective inhibitor of lysosomal cathepsin L and protects keratinocytes from ultraviolet-induced apoptosis

scientific article

IFI60/ISG60/IFIT4, a new member of the human IFI54/IFIT2 family of interferon-stimulated genes.

scientific article

IL-37 requires the receptors IL-18Rα and IL-1R8 (SIGIRR) to carry out its multifaceted anti-inflammatory program upon innate signal transduction

scientific article

Identification of a novel domain in two mammalian inositol-polyphosphate 5-phosphatases that mediates membrane ruffle localization. The inositol 5-phosphatase skip localizes to the endoplasmic reticulum and translocates to membrane ruffles ...

scientific article

Importance of the P4' residue in human granzyme B inhibitors and substrates revealed by scanning mutagenesis of the proteinase inhibitor 9 reactive center loop

scientific article

Importance of the prime subsites of the C1s protease of the classical complement pathway for recognition of substrates

scientific article published in December 2003

Inhibitory activity of a heterochromatin-associated serpin (MENT) against papain-like cysteine proteinases affects chromatin structure and blocks cell proliferation

scientific article

Introduction of a mutation in the shutter region of antithrombin (Phe77 --> Leu) increases affinity for heparin and decreases thermal stability

scientific article published in September 2003

Leucine-rich repeats 2-4 (Leu60-Glu128) of platelet glycoprotein Ibalpha regulate shear-dependent cell adhesion to von Willebrand factor.

scientific article published on 14 June 2006

MUSTANG-MR structural sieving server: applications in protein structural analysis and crystallography

scientific article

MUSTANG: a multiple structural alignment algorithm

scientific article published in August 2006

Managing and mining protein crystallization data.

scientific article

Maspin (SERPINB5) is an obligate intracellular serpin

scientific article

Maspin is not required for embryonic development or tumour suppression

scientific article

Maternal Torso-Like Coordinates Tissue Folding During Drosophila Gastrulation

scientific article published on 11 May 2017

Mechanisms of serpin dysfunction in disease.

scientific article

Mechanistic characterization and crystal structure of a small molecule inactivator bound to plasminogen activator inhibitor-1

scientific article

Mice Lacking Brinp2 or Brinp3, or Both, Exhibit Behaviors Consistent with Neurodevelopmental Disorders.

scientific article

Modeling of serpin-protease complexes: antithrombin-thrombin, alpha 1-antitrypsin (358Met-->Arg)-thrombin, alpha 1-antitrypsin (358Met-->Arg)-trypsin, and antitrypsin-elastase

scientific article published on 01 November 1996

Modulation of the proteolytic activity of the complement protease C1s by polyanions: implications for polyanion-mediated acceleration of interaction between C1s and SERPING1.

scientific article

Molecular basis of α1-antitrypsin deficiency revealed by the structure of a domain-swapped trimer

scientific article

Molecular contortionism - on the physical limits of serpin 'loop-sheet' polymers

scientific article published on August 2010

Molecular determinants of the mechanism underlying acceleration of the interaction between antithrombin and factor Xa by heparin pentasaccharide

scientific article published on 19 February 2002

Molecular gymnastics: serpin structure, folding and misfolding

scientific article

MrGrid: a portable grid based molecular replacement pipeline

scientific article

Multiple alignment and sorting of peptides derived from phage-displayed random peptide libraries with polyclonal sera allows discrimination of relevant phagotopes

scientific article

N-terminal domain of Bothrops asper Myotoxin II Enhances the Activity of Endothelin Converting Enzyme-1 and Neprilysin

scientific article

New insights into the structure and function of the plasminogen/plasmin system.

scientific article

PROSPER: an integrated feature-based tool for predicting protease substrate cleavage sites

scientific article

Perforin and granzymes: function, dysfunction and human pathology

scientific article published on June 2015

Perforin evolved from a gene duplication of MPEG1, followed by a complex pattern of gene gain and loss within Euteleostomi

scientific article

Perforin forms transient pores on the target cell plasma membrane to facilitate rapid access of granzymes during killer cell attack

scientific article published in February 2013

Perforin proteostasis is regulated through its C2 domain: supra-physiological cell death mediated by T431D-perforin.

scientific article published on 7 February 2018

Perforin-A key (shaped) weapon in the immunological arsenal.

scientific article

Perforin: structure, function, and role in human immunopathology

scientific article

PhosContext2vec: a distributed representation of residue-level sequence contexts and its application to general and kinase-specific phosphorylation site prediction.

scientific article

Phylogeny of the serpin superfamily: implications of patterns of amino acid conservation for structure and function.

scientific article

Plasmodium falciparum neutral aminopeptidases: new targets for anti-malarials

scientific article published on 30 September 2009

PoPS: a computational tool for modeling and predicting protease specificity

scientific article published in June 2005

Predicting giant transmembrane β-barrel architecture

scientific article

Predicting serpin/protease interactions

scientific article

Prediction of protein function from protein sequence and structure

scientific article

Preferential Acquisition and Activation of Plasminogen Glycoform II by PAM Positive Group A Streptococcal Isolates.

scientific article

Probing the role of the F-helix in serpin stability through a single tryptophan substitution

scientific article

Probing the unfolding pathway of alpha1-antitrypsin

scientific article published in April 1999

Prodepth: predict residue depth by support vector regression approach from protein sequences only

scientific article

Progressive multiple alignment using sequence triplet optimizations and three-residue exchange costs

scientific article published in December 2004

Protecting a serial killer: pathways for perforin trafficking and self-defence ensure sequential target cell death

scientific article published on 18 May 2012

Protection from endogenous perforin: glycans and the C terminus regulate exocytic trafficking in cytotoxic lymphocytes

scientific article published on 9 June 2011

RAB-like 2 has an essential role in male fertility, sperm intra-flagellar transport, and tail assembly

scientific article

RCPdb: An evolutionary classification and codon usage database for repeat-containing proteins

scientific article published on 13 June 2007

REFOLD: an analytical database of protein refolding methods

scientific article published on 15 August 2005

Real-time visualization of perforin nanopore assembly.

scientific article published on 6 February 2017

Reconciling the Structural Attributes of Avian Antibodies

scientific article

Regulation of perforin activation and pre-synaptic toxicity through C-terminal glycosylation

scientific article published on 14 August 2017

Role of the M-loop and reactive center loop domains in the folding and bridging of nucleosome arrays by MENT.

scientific article

S1 Pocket of a Bacterially Derived Subtilisin-like Protease Underpins Effective Tissue Destruction

scientific article

SERPINB11 is a new noninhibitory intracellular serpin. Common single nucleotide polymorphisms in the scaffold impair conformational change

scientific article

SH3 domains in prokaryotes

scientific article

SerpinB6 is an inhibitor of kallikrein-8 in keratinocytes

scientific article

Serpins 2005 - fun between the beta-sheets. Meeting report based upon presentations made at the 4th International Symposium on Serpin Structure, Function and Biology (Cairns, Australia).

scientific article published in October 2005

Serpins flex their muscle: I. Putting the clamps on proteolysis in diverse biological systems

scientific article

Serpins flex their muscle: II. Structural insights into target peptidase recognition, polymerization, and transport functions

scientific article

Serpins' mystery solved

scientific article published in Nature

Serpins: finely balanced conformational traps

scientific article

Smoothing a rugged protein folding landscape by sequence-based redesign

scientific article published on 26 September 2016

Stability of the octameric structure affects plasminogen-binding capacity of streptococcal enolase

scientific article published on 25 March 2015

Stonefish toxin defines an ancient branch of the perforin-like superfamily.

scientific article published in December 2015

Structural Basis for Ca2+-mediated Interaction of the Perforin C2 Domain with Lipid Membranes

scientific article

Structural and Functional Analysis of the Pore-Forming Toxin NetB from Clostridium perfringens

scientific article

Structural and functional analysis of the Josephin domain of the polyglutamine protein ataxin-3

article

Structural basis for the inhibition of the essential Plasmodium falciparum M1 neutral aminopeptidase

scientific article

Structural biology: Torqueing about pores.

scientific article

Structural characterization of the mechanism through which human glutamic acid decarboxylase auto-activates

scientific article

Structural determinants of GAD antigenicity

scientific article published on 23 September 2009

Structural insights into multi-protein communication systems

scientific article published in June 2012

Structural mechanisms of inactivation in scabies mite serine protease paralogues

scientific article

Structure of granzyme C reveals an unusual mechanism of protease autoinhibition

scientific article

Structure of the Plasmodium falciparum M17 aminopeptidase and significance for the design of drugs targeting the neutral exopeptidases

scientific article

Structure of the poly-C9 component of the complement membrane attack complex

scientific journal article

Subunit gamma-green fluorescent protein fusions are functionally incorporated into mitochondrial F1F0-ATP synthase, arguing against a rigid cap structure at the top of F1.

scientific article published on 31 October 2002

Synthesis of New (−)-Bestatin-Based Inhibitor Libraries Reveals a Novel Binding Mode in the S1 Pocket of the Essential Malaria M1 Metalloaminopeptidase

scientific article

T cell receptor recognition of a 'super-bulged' major histocompatibility complex class I-bound peptide

scientific article published on 25 September 2005

TcpM: a novel relaxase that mediates transfer of large conjugative plasmids from Clostridium perfringens

scientific article

Temperature sensitivity of human perforin mutants unmasks subtotal loss of cytotoxicity, delayed FHL, and a predisposition to cancer

scholarly article

The 1.5 A crystal structure of a prokaryote serpin: controlling conformational change in a heated environment

scientific article (publication date: April 2003)

The 1.6 A crystal structure of the catalytic domain of PlyB, a bacteriophage lysin active against Bacillus anthracis

scientific article

The 2.6 A structure of antithrombin indicates a conformational change at the heparin binding site

scientific article

The AprV5 subtilase is required for the optimal processing of all three extracellular serine proteases from Dichelobacter nodosus.

scientific article

The Extracellular Protein Factor Epf from Streptococcus pyogenes Is a Cell Surface Adhesin That Binds to Cells through an N-terminal Domain Containing a Carbohydrate-binding Module

scientific article

The FxRxHrS motif: a conserved region essential for DNA binding of the VirR response regulator from Clostridium perfringens

scientific article

The MACPF/CDC family of pore-forming toxins

scientific article

The N terminus of the serpin, tengpin, functions to trap the metastable native state

scientific article

The Phosphatidylinositol (3,4,5)-Trisphosphate-dependent Rac Exchanger 1·Ras-related C3 Botulinum Toxin Substrate 1 (P-Rex1·Rac1) Complex Reveals the Basis of Rac1 Activation in Breast Cancer Cells

scientific article

The REFOLD database: a tool for the optimization of protein expression and refolding

scientific article

The Structural Basis for Complement Inhibition by Gigastasin, a Protease Inhibitor from the Giant Amazon Leech.

scientific article

The Subtilisin-Like Protease AprV2 Is Required for Virulence and Uses a Novel Disulphide-Tethered Exosite to Bind Substrates

scientific article

The Three-dimensional Structure of the Extracellular Adhesion Domain of the Sialic Acid-binding Adhesin SabA fromHelicobacter pylori

scientific article published on 7 March 2014

The X-Ray Crystal Structure of Escherichia coli Succinic Semialdehyde Dehydrogenase; Structural Insights into NADP+/Enzyme Interactions

scientific article

The X-ray Crystal Structure of Mannose-binding Lectin-associated Serine Proteinase-3 Reveals the Structural Basis for Enzyme Inactivity Associated with the Carnevale, Mingarelli, Malpuech, and Michels (3MC) Syndrome

scientific article

The X-ray crystal structure of full-length human plasminogen

scientific article

The conjugation protein TcpC from Clostridium perfringens is structurally related to the type IV secretion system protein VirB8 from Gram-negative bacteria

scientific article

The crystal structure of DehI reveals a new alpha-haloacid dehalogenase fold and active-site mechanism

scientific article

The crystal structure of the transthyretin-like protein from Salmonella dublin, a prokaryote 5-hydroxyisourate hydrolase.

scientific article published on 11 May 2006

The evolution of enzyme specificity in Fasciola spp.

scientific article published in July 2003

The first transmembrane region of complement component-9 acts as a brake on its self-assembly

scientific article published in Nature Communications

The high resolution crystal structure of a native thermostable serpin reveals the complex mechanism underpinning the stressed to relaxed transition

scientific article

The high resolution crystal structure of the human tumor suppressor maspin reveals a novel conformational switch in the G-helix

scientific article

The human serpin proteinase inhibitor-9 self-associates at physiological temperatures

scientific article published on July 2004

The major human and mouse granzymes are structurally and functionally divergent

scientific article

The matrix refolded

article

The molecular basis for perforin oligomerization and transmembrane pore assembly

scientific article

The murine orthologue of human antichymotrypsin: a structural paradigm for clade A3 serpins

scientific article

The perforin pore facilitates the delivery of cationic cargos

scientific article published on 20 February 2014

The role of strand 1 of the C beta-sheet in the structure and function of alpha(1)-antitrypsin

scientific article

The serpin SQN-5 is a dual mechanistic-class inhibitor of serine and cysteine proteinases

scientific article published in March 2002

The serpins are an expanding superfamily of structurally similar but functionally diverse proteins. Evolution, mechanism of inhibition, novel functions, and a revised nomenclature

scientific article (publication date: 7 September 2001)

The structural basis for membrane binding and pore formation by lymphocyte perforin

scientific article

The structure and function of catalytic domains within inositol polyphosphate 5-phosphatases.

scientific article

The structure and function of mammalian membrane-attack complex/perforin-like proteins.

scientific article published on 22 September 2010

The structure of chagasin in complex with a cysteine protease clarifies the binding mode and evolution of an inhibitor family

scientific article

The type III effectors NleE and NleB from enteropathogenic E. coli and OspZ from Shigella block nuclear translocation of NF-kappaB p65.

scientific article

The type Ialpha inositol polyphosphate 4-phosphatase generates and terminates phosphoinositide 3-kinase signals on endosomes and the plasma membrane

scientific article

The x-ray crystal structure of mannose-binding lectin-associated serine proteinase-3 reveals the structural basis for enzyme inactivity associated with the Carnevale, Mingarelli, Malpuech, and Michels (3MC) syndrome.

scientific article published in September 2013

The yeast inositol polyphosphate 5-phosphatase Inp54p localizes to the endoplasmic reticulum via a C-terminal hydrophobic anchoring tail: regulation of secretion from the endoplasmic reticulum.

scientific article

Thermal Unfolding of Proteins Studied by Coupled Reversed-Phase HPLC−Electrospray Ionization Mass Spectrometry Techniques Based on Isotope Exchange Effects

scientific article published on 01 March 2002

Torso-Like Is a Component of the Hemolymph and Regulates the Insulin Signalling Pathway in Drosophila

scientific article published on 13 February 2018

Torso-like functions independently of Torso to regulate Drosophila growth and developmental timing

scientific article published on 19 August 2013

Torso-like mediates extracellular accumulation of Furin-cleaved Trunk to pattern the Drosophila embryo termini

scientific article

Tranexamic acid is an active site inhibitor of urokinase plasminogen activator

scientific article published on 01 March 2019

Trunk cleavage is essential for Drosophila terminal patterning and can occur independently of Torso-like

scientific article published on 3 March 2014

X-ray crystal structure and specificity of the Plasmodium falciparum malaria aminopeptidase PfM18AAP

scientific article

X-ray crystal structure of MENT: evidence for functional loop-sheet polymers in chromatin condensation

scientific article

X-ray crystal structure of plasmin with tranexamic acid-derived active site inhibitors

scientific article published on 09 May 2017

X-ray crystal structure of the fibrinolysis inhibitor alpha2-antiplasmin

scientific article

X-ray crystal structure of the streptococcal specific phage lysin PlyC. ⬇️

scientific article

alpha 1-Antitrypsin Mmalton (Phe52-deleted) forms loop-sheet polymers in vivo. Evidence for the C sheet mechanism of polymerization

scientific article published in July 1995

α1-Antitrypsin Polymerisation Can Occur by both Loop A and C Sheet Mechanisms

scientific article published on 01 October 1998

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