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List of works by Jan Steyaert

1,2,3-Triazolylalkylribitol derivatives as nucleoside hydrolase inhibitors

scientific article published on 09 February 2007

A Decade of Protein Engineering on Ribonuclease T1— Atomic Dissection of the Enzyme‐Substrate Interactions ⬇️

scientific article published on July 1, 1997

A Genetically Encoded Biosensor Reveals Location Bias of Opioid Drug Action.

scientific article

A Two-Binding-Site Kinetic Model for the Ribonuclease-T1-Catalysed Transesterification of Dinucleoside Phosphate Substrates

article

A catalytic function for the structurally conserved residue Phe 100 of ribonuclease T1.

scientific article

A flexible loop as a functional element in the catalytic mechanism of nucleoside hydrolase from Trypanosoma vivax

scientific article

A general protocol for the generation of Nanobodies for structural biology

scientific article

A lipid site shapes the agonist response of a pentameric ligand-gated ion channel

scientific article published on 07 October 2019

A llama-derived gelsolin single-domain antibody blocks gelsolin-G-actin interaction

scientific article

A novel expression system for production of soluble prion proteins in E. coli

scientific article published on 10 January 2012

A nucleophile activation dyad in ribonucleases. A combined X-ray crystallographic/ab initio quantum chemical study

scientific article

A specific nanobody prevents amyloidogenesis of D76N β2-microglobulin in vitro and modifies its tissue distribution in vivo.

scientific article

A structure of substrate-bound Synaptojanin1 provides new insights in its mechanism and the effect of disease mutations

scientific article published on 22 December 2020

Activation and allosteric modulation of a muscarinic acetylcholine receptor

scientific article

Additivity of protein-guanine interactions in ribonuclease T1

scientific article

Allosteric coupling from G protein to the agonist-binding pocket in GPCRs

scientific article published on 29 June 2016

Allosteric inhibition of VIM metallo-β-lactamases by a camelid nanobody

scientific article

Allosteric modulation of the GTPase activity of a bacterial LRRK2 homolog by conformation-specific Nanobodies

scientific article published on 01 April 2020

Allosteric nanobodies reveal the dynamic range and diverse mechanisms of G-protein-coupled receptor activation

scientific article published on 13 July 2016

An engineered ribonuclease preferring phosphorothioate RNA

scientific article published on 01 May 1998

An improved yeast surface display platform for the screening of nanobody immune libraries

scientific article published in Scientific Reports

An intrabody based on a llama single-domain antibody targeting the N-terminal alpha-helical multimerization domain of HIV-1 rev prevents viral production.

scientific article

Analysis of a Water Mediated Protein−Protein Interactions within RNase T1†,‡

article

Antibodies targeting G protein-coupled receptors: Recent advances and therapeutic challenges.

scientific article published on 5 May 2017

Assessment of stability, toxicity and immunogenicity of new polymeric nanoreactors for use in enzyme replacement therapy of MNGIE.

scientific article

Atomic structure of a nanobody-trapped domain-swapped dimer of an amyloidogenic  2-microglobulin variant

scientific article

BRET evidence that β2 adrenergic receptors do not oligomerize in cells

scientific article published on 8 May 2015

Binding Specificities of Nanobody•Membrane Protein Complexes Obtained from Chemical Cross-Linking and High-Mass MALDI Mass Spectrometry.

scientific article published on 21 March 2018

Binding-Site Compatible Fragment Growing Applied to the Design of β2-Adrenergic Receptor Ligands

scientific article

Catalysis by nucleoside hydrolases

scientific article published on December 2003

Challenges in the Structural-Functional Characterization of Multidomain, Partially Disordered Proteins CBP and p300: Preparing Native Proteins and Developing Nanobody Tools

scientific article published on 03 November 2018

Characterisation and structure determination of a llama-derived nanobody targeting the J-base binding protein 1

Characterization and structure determination of a llama-derived nanobody targeting the J-base binding protein 1

Characterization of phenylpyruvate decarboxylase, involved in auxin production of Azospirillum brasilense

scientific article published on 31 August 2007

Characterization of two homologous 2′-O-methyltransferases showing different specificities for their tRNA substrates

scientific article

Cloning, preliminary characterization and crystallization of nucleoside hydrolases from Caenorhabditis elegans and Campylobacter jejuni

scientific article published on 23 May 2003

Combining in-situ proteolysis and microseed matrix screening to promote crystallization of PrPc-nanobody complexes

scientific article published on 01 May 2011

Conformational biosensors reveal GPCR signalling from endosomes.

scientific article

Conformational stability and activity of ribonuclease T1 and mutants. Gln25----Lys, Glu58----Ala, and the double mutant.

scientific article

Conserved water molecules in a large family of microbial ribonucleases

scientific article

Constraining enzyme conformational change by an antibody leads to hyperbolic inhibition

scientific article

Correction to Discovery, Structure–Activity Relationship, and Binding Mode of an Imidazo[1,2-a]pyridine Series of Autotaxin Inhibitors

Cryo-EM structure of the human α1β3γ2 GABA receptor in a lipid bilayer

Crystal Structure of a ligand-bound LacY-Nanobody Complex

article published in the Proceedings of the National Academy of Sciences of the United States of America

Crystal Structure of the N-Terminal Domain of the Secretin GspD from ETEC Determined with the Assistance of a Nanobody

scientific article

Crystal Structures of the Human Doublecortin C- and N-terminal Domains in Complex with Specific Antibodies

scientific article published on 10 May 2016

Crystal structure determination and refinement at 2.3-.ANG. resolution of the lentil lectin. [Erratum to document cited in CA119(17):176148u]

scientific article published on 01 December 1993

Crystal structure determination and refinement at 2.3-A resolution of the lentil lectin

scientific article

Crystal structure of a LacY-nanobody complex in a periplasmic-open conformation

scientific article published on 19 October 2016

Crystal structure of a SLC11 (NRAMP) transporter reveals the basis for transition-metal ion transport

scientific article

Crystal structure of a heterodimer of editosome interaction proteins in complex with two copies of a cross-reacting nanobody

scientific article

Crystal structure of human Mediator subunit MED23

scientific article published in Nature Communications

Crystal structure of the Bloom's syndrome helicase indicates a role for the HRDC domain in conformational changes

scientific article

Crystal structure of the proteasomal deubiquitylation module Rpn8-Rpn11

scientific article

Crystal structure of the β2 adrenergic receptor-Gs protein complex

scientific article

Crystal structures of T. vivax nucleoside hydrolase in complex with new potent and specific inhibitors

scientific article

Crystallization and preliminary X-ray diffraction analysis of a specific VHH domain against mouse prion protein

scientific article published on 26 November 2010

Crystallographic Study of Glu58Ala RNase T1.cntdot.2'-Guanosine Monophosphate at 1.9-.ANG. Resolution

scientific article published in 1994

Crystallographic and biochemical characterization of the dimeric architecture of site-2 protease

scientific article published on 11 May 2017

DNA immunization combined with scFv phage display identifies antagonistic GCGR specific antibodies and reveals new epitopes on the small extracellular loops

scientific article published on 21 May 2016

Deciphering the Mechanism of RNase T1

article

Development by Genetic Immunization of Monovalent Antibodies (Nanobodies) Behaving as Antagonists of the Human ChemR23 Receptor

scientific article published on 10 February 2016

Development by Genetic Immunization of Monovalent Antibodies Against Human Vasoactive Intestinal Peptide Receptor 1 (VPAC1), New Innovative, and Versatile Tools to Study VPAC1 Receptor Function

scientific article published on 5 April 2018

Development of a universal nanobody-binding Fab module for fiducial-assisted cryo-EM studies of membrane proteins

scientific article published on 01 November 2021

Discovery, Structure-Activity Relationship, and Binding Mode of an Imidazo[1,2-a]pyridine Series of Autotaxin Inhibitors

scientific article published on 21 July 2017

Dissecting histidine interactions of ribonuclease T1 with asparagine and glutamine replacements: analysis of double mutant cycles at one position

scientific article

Dissection of the ribonuclease T1 subsite. The transesterification kinetics of Asn36Ala and Asn98Ala ribonuclease T1 for minimal dinucleoside phosphates

scientific article published on 01 February 1992

Dissection of the structural and functional role of a conserved hydration site in RNase T1

scientific article

Distinct conformations of GPCR-β-arrestin complexes mediate desensitization, signaling, and endocytosis

scientific article

Diversity in kinetics correlated with structure in nano body-stabilized LacY

scientific article published on 07 May 2020

Domain-interface dynamics of CFTR revealed by stabilizing nanobodies.

scientific article published on 14 June 2019

Editorial overview: Engineered proteins as tools in structural biology

scientific article published on 01 February 2020

Encapsulation of therapeutic nucleoside hydrolase in functionalised nanocapsules

Enzyme-substrate interactions in the purine-specific nucleoside hydrolase from Trypanosoma vivax

scientific article

Erratum: Corrigendum: Structure of a prokaryotic fumarate transporter reveals the architecture of the SLC26 family

scholarly article published in Nature Structural & Molecular Biology

Evaluation of nucleoside hydrolase inhibitors for treatment of African trypanosomiasis

scientific article

Examination of the mechanism and energetic contribution of leaving group activation in the purine-specific nucleoside hydrolase from Trypanosoma vivax

scientific article published on 6 September 2007

Expression, purification, crystallization and preliminary X-ray analysis of cyclophilin A from the bovine parasite Trypanosoma brucei brucei

scientific article published on 01 September 1998

Functional Interactions among the His40, Glu58 and His92 Catalysts of Ribonuclease T1 as Studied by Double and Triple Mutants

Functional and Biochemical Characterization of Alvinella pompejana Cys-Loop Receptor Homologues

scientific article

Functional assessment of ?in vivo? and ?in silico? mutations in the guanine binding site of RNase T1: A DFT study

GABA receptor signalling mechanisms revealed by structural pharmacology

scientific article published on 02 January 2019

Generation and Characterization of Anti-VGLUT Nanobodies Acting as Inhibitors of Transport.

scientific article published on 21 July 2017

Heteromeric GABAAreceptor structures in positively-modulated active states

Histidine-40 of ribonuclease T1 acts as base catalyst when the true catalytic base, glutamic acid-58, is replaced by alanine

scientific article published in September 1990

Hydrogen-exchange stabilities of RNase T1 and variants with buried and solvent-exposed Ala --> Gly mutations in the helix

scientific article

Hydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallography

scientific article

Hydrophobic core manipulations in ribonuclease T1

scientific article

In vitro reconstitution of dynamically interacting integral membrane subunits of energy-coupling factor transporters

scientific article published on 22 December 2020

Influence of the pi-pi interaction on the hydrogen bonding capacity of stacked DNA/RNA bases

scientific article

Inhibition of Ligand Exchange Kinetics via Active-Site Trapping with an Antibody Fragment

scientific article published in 2014

Internalized Receptor for Glucose-dependent Insulinotropic Peptide stimulates adenylyl cyclase on early endosomes

scientific article

Interrogating dense ligand chemical space with a forward-synthetic library

scientific article published on 21 May 2019

Investigation of the functional interplay between the primary site and the subsite of RNase T1: Kinetic analysis of single and multiple mutants for modified substrates

scholarly article by Jan Steyaert published in April 1994

Isolation of antigen-binding camelid heavy chain antibody fragments (nanobodies) from an immune library displayed on the surface of Pichia pastoris

scientific article

L amino acid transporter structure and molecular bases for the asymmetry of substrate interaction

scientific article published on 18 April 2019

Leaving group activation by aromatic stacking: an alternative to general acid catalysis

scientific article published in April 2004

Leishmania donovani tyrosyl-tRNA synthetase structure in complex with a tyrosyl adenylate analog and comparisons with human and protozoan counterparts

scientific article published on 18 April 2017

Llama immunization with full-length VAR2CSA generates cross-reactive and inhibitory single-domain antibodies against the DBL1X domain

scientific article published on 09 December 2014

Mapping inhibitory sites on the RNA polymerase of the 1918 pandemic influenza virus using nanobodies

scientific article published on 11 January 2022

Mapping the binding interface between an HIV-1 inhibiting intrabody and the viral protein Rev

scientific article

Mapping the conformational space accessible to BACE2 using surface mutants and cocrystals with Fab fragments, Fynomers and Xaperones

scientific article

Measuring cooperative Rev protein-protein interactions on Rev responsive RNA by fluorescence resonance energy transfer

scientific article

Mechanism of RNase T1: concerted triester-like phosphoryl transfer via a catalytic three-centered hydrogen bond

scientific article published on 01 August 2000

Mechanistic analysis of allosteric and non-allosteric effects arising from nanobody binding to two epitopes of the dihydrofolate reductase of Escherichia coli

scientific article

Modular Integrated Secretory System Engineering in Pichia pastoris To Enhance G-Protein Coupled Receptor Expression

scientific article

Modulation of the Erwinia ligand-gated ion channel (ELIC) and the 5-HT3 receptor via a common vestibule site

scientific article published on 28 January 2020

Molecular mechanism of allosteric substrate activation in a thiamine diphosphate-dependent decarboxylase

scientific article

Multiple transients in the pre-steady-state of nucleoside hydrolase reveal complex substrate binding, product base release, and two apparent rates of chemistry

scientific article published on August 2006

Mutational analysis of the extracellular disulphide bridges of the atypical chemokine receptor ACKR3/CXCR7 uncovers multiple binding and activation modes for its chemokine and endogenous non-chemokine agonists.

scientific article published on 9 March 2018

N-Arylmethyl substituted iminoribitol derivatives as inhibitors of a purine specific nucleoside hydrolase

scientific article published on 18 June 2008

Nanobodies Raised against Monomeric α-Synuclein Distinguish between Fibrils at Different Maturation Stages

scientific article published on 01 April 2013

Nanobodies to Study G Protein-Coupled Receptor Structure and Function

scientific article

Nanobodies to study protein conformational states

scientific article published on 06 February 2020

Nanobody mediated crystallization of an archeal mechanosensitive channel.

scientific article

Nanobody stabilization of G protein-coupled receptor conformational states ⬇️

scientific article published on July 21, 2011

Nanobody-Enabled Reverse Pharmacology on G-Protein-Coupled Receptors

scientific article published on 22 February 2018

Nanobody-Enabled Reverse Pharmacology on G-Protein-Coupled Receptors

scholarly article by Els Pardon et al published 30 March 2018 in Angewandte Chemie

Nanobody-aided crystallization of the transcription regulator PaaR2 from <i>Escherichia coli</i> O157:H7

scientific article published in 2021

Nanobody-aided structure determination of the EpsI:EpsJ pseudopilin heterodimer from Vibrio vulnificus

scientific article

Nanobody-enabled monitoring of kappa opioid receptor states

scientific article published on 02 March 2020

New insights into the mechanism of nucleoside hydrolases from the crystal structure of the Escherichia coli YbeK protein bound to the reaction product

scientific article published on January 2006

Outward-facing conformers of LacY stabilized by nanobodies

scientific article

Polymeric nanoreactors for enzyme replacement therapy of MNGIE.

scientific article published on November 2010

Pre-steady-state analysis of the nucleoside hydrolase of Trypanosoma vivax. Evidence for half-of-the-sites reactivity and rate-limiting product release.

scientific article published in November 2003

Primary structure and partial characterization of a life-cycle-regulated cysteine protease from Trypanosoma (Nannomonas) congolense

scientific article published on August 1995

Probing the N-terminal β-sheet conversion in the crystal structure of the human prion protein bound to a nanobody

scientific article

Production, crystallization and X-ray diffraction analysis of two nanobodies against the Duffy binding-like (DBL) domain DBL6ℇ-FCR3 of the Plasmodium falciparum VAR2CSA protein.

scientific article published on 22 February 2013

Production, crystallization and X-ray diffraction analysis of two nanobodies against the Duffy binding-like (DBL) domain DBL6∊-FCR3 of the Plasmodium falciparum VAR2CSA protein. Corrigendum

scientific article published on 31 October 2013

Production, crystallization and preliminary X-ray diffraction of the Gαs α-helical domain in complex with a nanobody

scientific article published on 25 October 2014

Propagation of conformational changes during μ-opioid receptor activation

scientific article published on 5 August 2015

Purification, circular dichroism analysis, crystallization and preliminary X-ray diffraction analysis of the F plasmid CcdB killer protein.

scientific article

Quantitative analysis of the contribution of Glu46 and Asn98 to the guanosine specificity of ribonuclease T1.

scientific article

Quantum chemical study of leaving group activation inT. vivax nucleoside hydrolase

article published in 2005

Quiescin-sulfhydryl oxidase inhibits prion formation in vitro

scientific article published on 11 December 2016

Rational Design of Nanobody80 Loop Peptidomimetics: Towards Biased β2 Adrenergic Receptor Ligands.

scientific article published on 27 April 2017

Recombinant human prion protein inhibits prion propagation in vitro

scientific article published on 09 October 2013

Reconsidering the energetics of ribonuclease catalysed RNA hydrolysis

scientific article published on 01 October 1998

Regulation of β2-adrenergic receptor function by conformationally selective single-domain intrabodies.

scientific article published on 06 December 2013

Ribonucleases: from prototypes to therapeutic targets?

scientific article

Role of histidine-40 in ribonuclease T1 catalysis: three-dimensionalstructures of the partially active His40Lys mutant

scientific article

SAXS analysis of the tRNA-modifying enzyme complex MnmE/MnmG reveals a novel interaction mode and GTP-induced oligomerization

scientific article

SbsB structure and lattice reconstruction unveil Ca2+ triggered S-layer assembly

scientific article

Soluble polymorphic bank vole prion proteins induced by co-expression of quiescin sulfhydryl oxidase in E. coli and their aggregation behaviors

scientific article published on 4 October 2017

Sortase A-mediated site-specific labeling of camelid single-domain antibody-fragments: a versatile strategy for multiple molecular imaging modalities.

scientific article published on 5 May 2016

Structural analysis of the interaction between spiroisoxazoline SMARt-420 and the Mycobacterium tuberculosis repressor EthR2.

scientific article published on 14 April 2017

Structural and Functional Studies on the Interaction of GspC and GspD in the Type II Secretion System

scientific article

Structural and biochemical characterization of the nucleoside hydrolase from C. elegans reveals the role of two active site cysteine residues in catalysis.

scientific article published on 20 February 2017

Structural basis for GABAA receptor potentiation by neurosteroids.

scientific article

Structural basis of inhibition of lipid-linked oligosaccharide flippase PglK by a conformational nanobody

scientific article

Structural evidence for the critical role of the prion protein hydrophobic region in forming an infectious prion

scientific article published on 09 December 2019

Structural flexibility of the G alpha s alpha-helical domain in the beta2-adrenoceptor Gs complex

scientific article

Structural insights into binding specificity, efficacy and bias of a βAR partial agonist

scientific article

Structural insights into the activation of metabotropic glutamate receptors

scientific article published on 23 January 2019

Structural insights into µ-opioid receptor activation

scientific article

Structure and Mechanism of Hedgehog Acyl Transferase

Structure and flexibility of the endosomal Vps34 complex reveals the basis of its function on membranes

scientific article

Structure and function of a novel purine specific nucleoside hydrolase from Trypanosoma vivax

scientific article

Structure and mechanism of the 6-oxopurine nucleosidase from Trypanosoma brucei brucei

scientific article

Structure and nucleotide-induced conformational dynamics of the Chlorobium tepidum Roco protein

scientific article published on 07 January 2019

Structure and properties of a complex of α-synuclein and a single-domain camelid antibody

scientific article

Structure of PINK1 in complex with its substrate ubiquitin.

scientific article published on 30 October 2017

Structure of Prototypic Peptide Transporter DtpA from E. coli in Complex with Valganciclovir Provides Insights into Drug Binding of Human PepT1

scientific article published on 31 January 2019

Structure of S-layer protein Sap reveals a mechanism for therapeutic intervention in anthrax

scientific article published on 15 July 2019

Structure of a bacterial type IV secretion core complex at subnanometre resolution

scientific article

Structure of a nanobody-stabilized active state of the β(2) adrenoceptor

scientific article

Structure of a prokaryotic fumarate transporter reveals the architecture of the SLC26 family

scientific article

Structure of an early native-like intermediate of β2-microglobulin amyloidogenesis

scientific article

Structure of an endosomal signaling GPCR-G protein-β-arrestin megacomplex

scientific article published on 18 November 2019

Structure of the G protein chaperone and guanine nucleotide exchange factor Ric-8A bound to Gαi1

scientific article published on 26 February 2020

Structure of the Nanobody-Stabilized Active State of the Kappa Opioid Receptor.

scientific article published on 3 January 2018

Structure, mechanism, and inhibition of Hedgehog acyltransferase

scientific article published on 09 December 2021

Structures of P-glycoprotein reveal its conformational flexibility and an epitope on the nucleotide-binding domain

scientific article

Structures of a key interaction protein from the Trypanosoma brucei editosome in complex with single domain antibodies

scientific article

Structures of influenza A virus RNA polymerase offer insight into viral genome replication

scientific article published on 04 September 2019

Structures of ligand-occupied β-Klotho complexes reveal a molecular mechanism underlying endocrine FGF specificity and activity

scientific article published on 03 April 2019

Structures of β-klotho reveal a 'zip code'-like mechanism for endocrine FGF signalling

scientific article published on 17 January 2018

Subsite interactions of ribonuclease T1: Asn36 and Asn98 accelerate GpN transesterification through interactions with the leaving nucleoside N.

scientific article published in September 1991

Subsite interactions of ribonuclease T1: viscosity effects indicate that the rate-limiting step of GpN transesterification depends on the nature of N.

scientific article published in September 1991

Substrate-assisted leaving group activation in enzyme-catalyzed N-glycosidic bond cleavage.

scientific article published on 28 January 2005

Substrate-dependent modulation of enzyme activity by allosteric effector antibodies

scientific article

Synthesis and biochemical evaluation of guanidino-alkyl-ribitol derivatives as nucleoside hydrolase inhibitors

scientific article published on 10 April 2007

Synthesis of bicyclic N-arylmethyl-substituted iminoribitol derivatives as selective nucleoside hydrolase inhibitors.

scientific article published in February 2009

Targeting G protein-coupled receptor signaling at the G protein level with a selective nanobody inhibitor.

scientific article

The F plasmid CcdB protein induces efficient ATP-dependent DNA cleavage by gyrase.

scientific article

The Molecular Mechanism of Transport by the Mitochondrial ADP/ATP Carrier

scientific article published on 02 January 2019

The contribution of metal ions to the conformational stability of ribonuclease T1: crystal versus solution

scientific article

The crystal structure of phenylpyruvate decarboxylase from Azospirillum brasilense at 1.5 A resolution. Implications for its catalytic and regulatory mechanism

scientific article

The morphogen Sonic hedgehog inhibits its receptor Patched by a pincer grasp mechanism

scientific article published on 23 September 2019

The structure of the C-terminal domain of the largest editosome interaction protein and its role in promoting RNA binding by RNA-editing ligase L2

scientific article

The structure of the D3 domain of Plasmodium falciparum myosin tail interacting protein MTIP in complex with a nanobody

scientific article

The unexpected structure of the designed protein Octarellin V.1 forms a challenge for protein structure prediction tools

scientific article

Therapeutic nanoreactors: combining chemistry and biology in a novel triblock copolymer drug delivery system.

scientific article published in November 2005

Thermodynamics of Nanobody Binding to Lactose Permease.

scientific article published on 30 September 2016

Transient conformers of LacY are trapped by nanobodies

scientific article

Transition-state complex of the purine-specific nucleoside hydrolase of T. vivax: enzyme conformational changes and implications for catalysis

scientific article published on 29 March 2006

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