List of works - Rolf Hilgenfeld

3C Protease of Enterovirus 68: Structure-Based Design of Michael Acceptor Inhibitors and Their Broad-Spectrum Antiviral Effects against Picornaviruses

scientific article

A G-quadruplex-binding macrodomain within the "SARS-unique domain" is essential for the activity of the SARS-coronavirus replication-transcription complex.

scientific article

A structural view of the inactivation of the SARS coronavirus main proteinase by benzotriazole esters

scientific article

Accessory proteins of SARS-CoV and other coronaviruses

scientific article published on July 2014

Acquisition of new protein domains by coronaviruses: analysis of overlapping genes coding for proteins N and 9b in SARS coronavirus

scientific article published on 20 November 2014

Alpha-ketoamides as broad-spectrum inhibitors of coronavirus and enterovirus replication

scientific article

An efficient method for the synthesis of peptide aldehyde libraries employed in the discovery of reversible SARS coronavirus main protease (SARS-CoV Mpro) inhibitors

scientific article published in July 2006

An overview on 2-methyl-2,4-pentanediol in crystallization and in crystals of biological macromolecules

scientific article published on 26 September 2002

An unfolding/refolding step helps in the crystallization of a poorly soluble protein

scientific article published on 27 June 2003

Antiviral Activity of Broad-Spectrum and Enterovirus-Specific Inhibitors against Clinical Isolates of Enterovirus D68.

scientific article

Antiviral strategies to control calicivirus infections

scientific article published on 21 May 2010

Application of a cell-based protease assay for testing inhibitors of picornavirus 3C proteases

scientific article published on 4 January 2014

Architecture and regulation of HtrA-family proteins involved in protein quality control and stress response ⬇️

scientific article published on July 18, 2012

Biochemical and functional analyses of the Mip protein: influence of the N-terminal half and of peptidylprolyl isomerase activity on the virulence of Legionella pneumophila

scientific article published on August 2003

Catalytic cleavage of HEAT and subsequent covalent binding of the tetralone moiety by the SARS-CoV-2 main protease

scientific article published on 05 May 2020

Characterization of an Allosteric Pocket in Zika Virus NS2B-NS3 Protease

scientific article published on 07 February 2022

Chemotherapeutic Effects of Bioassay-Guided Extracts of the American Cockroach, Periplaneta americana ⬇️

scientific article published on July 6, 2011

Computer-Aided Structure Based Drug Design Approaches for the Discovery of New Anti-CHIKV Agents

scientific article published on 9 March 2017

Conformational antagonism between opposing active sites in a bifunctional RelA/SpoT homolog modulates (p)ppGpp metabolism during the stringent response [corrected].

scientific article published in April 2004

Coronavirus main proteinase (3CLpro) structure: basis for design of anti-SARS drugs

scientific article (publication date: 13 June 2003)

Correction: Design of Wide-Spectrum Inhibitors Targeting Coronavirus Main Proteases

scientific article

Coxsackievirus B3 protease 3C: expression, purification, crystallization and preliminary structural insights

scientific article

Crystal Structure of the Peroxo-diiron(III) Intermediate of Deoxyhypusine Hydroxylase, an Oxygenase Involved in Hypusination

scientific article

Crystal structure of SARS-CoV-2 main protease provides a basis for design of improved α-ketoamide inhibitors

scientific article from

Crystal structure of Zika virus NS2B-NS3 protease in complex with a boronate inhibitor

scientific article

Crystal structure of the C-terminal fragment of NS1 protein from yellow fever virus

scientific article

Crystal structure of the middle domain of human poly(A)-binding protein-interacting protein 1 ⬇️

scientific article published on April 23, 2011

Crystal structure of the papain-like protease of MERS coronavirus reveals unusual, potentially druggable active-site features

scientific article

Crystal structures of the X-domains of a Group-1 and a Group-3 coronavirus reveal that ADP-ribose-binding may not be a conserved property

scientific article

Crystallization comes of age

scientific article published on 01 August 2002

Design of wide-spectrum inhibitors targeting coronavirus main proteases

scientific article

Development and Characterization of New Peptidomimetic Inhibitors of the West Nile Virus NS2B–NS3 Protease ⬇️

scientific article published on January 10, 2013

Effects of NS2B-NS3 protease and furin inhibition on West Nile and Dengue virus replication

scientific article published on December 2017

Erratum to: Liberation of SARS-CoV main protease from the viral polyprotein: N-terminal autocleavage does not depend on the mature dimerization mode.

scientific article published in March 2010

Erratum: Crystal structure of active elongation factor Tu reveals major domain rearrangements

scientific article published in Nature

Evidence for a Two-Metal-Ion Mechanism in the Cytidyltransferase KdsB, an Enzyme Involved in Lipopolysaccharide Biosynthesis ⬇️

scientific article published on August 3, 2011

Exoerythrocytic Plasmodium parasites secrete a cysteine protease inhibitor involved in sporozoite invasion and capable of blocking cell death of host hepatocytes

scientific article

Extended substrate specificity and first potent irreversible inhibitor/activity-based probe design for Zika virus NS2B-NS3 protease

scientific article (publication date: December 2016)

From SARS to MERS: 10 years of research on highly pathogenic human coronaviruses

scientific article published on 06 September 2013

From SARS to MERS: crystallographic studies on coronaviral proteases enable antiviral drug design

scientific article

Functional Characterization of the Cleavage Specificity of the Sapovirus Chymotrypsin-Like Protease

scientific article (publication date: August 2008)

Helix Unfolding/Refolding Characterizes the Functional Dynamics of Staphylococcus aureus Clp Protease ⬇️

scientific article published on April 26, 2013

Human glutamate carboxypeptidase II inhibition: structures of GCPII in complex with two potent inhibitors, quisqualate and 2-PMPA

scientific article

Identification of novel falcipain-2 inhibitors as potential antimalarial agents through structure-based virtual screening.

scientific article published in August 2009

Improved synthesis of rupintrivir

scientific article published on 04 January 2012

Improving experimental phases for strong reflections prior to density modification

scientific article published on 20 September 2013

Irreversible inhibitors of the 3C protease of Coxsackie virus through templated assembly of protein-binding fragments

scientific article published on 28 September 2016

Liberation of SARS-CoV main protease from the viral polyprotein: N-terminal autocleavage does not depend on the mature dimerization mode ⬇️

scientific article published on February 7, 2010

Lybatides from Lycium barbarum Contain An Unusual Cystine-stapled Helical Peptide Scaffold

scientific article

Modern methods of drug discovery: an introduction

scientific article

Molecular mechanisms of severe acute respiratory syndrome (SARS)

scientific article

Non structural proteins 8 and 9 of human coronavirus 229E.

scientific article

Nonstructural Proteins 7 and 8 of Feline Coronavirus Form a 2:1 Heterotrimer That Exhibits Primer-Independent RNA Polymerase Activity

scientific article

Nonstructural proteins of human coronavirus NL63.

scientific article published in January 2006

Nsp3 of coronaviruses: Structures and functions of a large multi-domain protein

scientific article published on 8 November 2017

Nucleocapsid protein of SARS coronavirus tightly binds to human cyclophilin A.

scientific article published in August 2004

Optimization of Protein Crystallization: The OptiCryst Project

Peptide aldehyde inhibitors challenge the substrate specificity of the SARS-coronavirus main protease

scientific article

Peptide-Boronic Acid Inhibitors of Flaviviral Proteases: Medicinal Chemistry and Structural Biology

scientific article published on 14 December 2016

PhyloMap: an algorithm for visualizing relationships of large sequence data sets and its application to the influenza A virus genome ⬇️

scientific article published on June 20, 2011

Picornavirus non-structural proteins as targets for new anti-virals with broad activity

scientific article

Processing of the SARS-CoV pp1a/ab nsp7-10 region

scientific article published on 01 March 2020

Processing of the SARS-CoV pp1a/ab nsp7-10 region

scientific article published on 03 December 2019

Production of coronavirus nonstructural proteins in soluble form for crystallization

scientific article

Production, crystallization and X-ray diffraction analysis of the protease CT441 from Chlamydia trachomatis

scientific article

Profiling of flaviviral NS2B-NS3 protease specificity provides a structural basis for the development of selective chemical tools that differentiate Dengue from Zika and West Nile viruses

scientific article published on 31 January 2020

Purification, characterization, and crystallization of alliinase from garlic

scientific article published in June 2002

RNA-virus proteases counteracting host innate immunity

scientific article

Recent Advances in Neuraminidase Inhibitor Development as Anti‐influenza Drugs ⬇️

scientific article published on July 16, 2012

Recent advances in targeting viral proteases for the discovery of novel antivirals

scientific article

SARS-CoV-2 Mpro inhibitors and activity-based probes for patient-sample imaging

scientific article published on 22 October 2020

STD-NMR experiments identify a structural motif with novel second-site activity against West Nile virus NS2B-NS3 protease

scientific article published on 16 September 2017

Sensitized detection of inhibitory fragments and iterative development of non-peptidic protease inhibitors by dynamic ligation screening

scientific article published on 01 January 2008

Single-drop optimization of protein crystallization ⬇️

scientific article published on July 31, 2012

Sometimes intermediates. Do the job!

scientific article

Structural and functional characterization of Falcipain-2, a hemoglobinase from the malarial parasite Plasmodium falciparum

scientific article published on 15 June 2006

Structural and mechanistic analysis of the membrane-embedded glycosyltransferase WaaA required for lipopolysaccharide synthesis

scientific article

Structural and mutational analysis of the interaction between the Middle-East respiratory syndrome coronavirus (MERS-CoV) papain-like protease and human ubiquitin

scientific article

Structural basis for the regulation of cysteine-protease activity by a new class of protease inhibitors in Plasmodium

scientific article

Structural basis of the proteolytic and chaperone activity of Chlamydia trachomatis CT441.

scientific article published on 27 October 2014

Structural biology in the fight against COVID-19

scientific article

Structure and cleavage specificity of the chymotrypsin-like serine protease (3CLSP/nsp4) of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)

scientific article

Structure and dynamics of SARS coronavirus main proteinase (Mpro)

scientific article published on 01 January 2006

Structure and functionality in flavivirus NS-proteins: perspectives for drug design

scientific article

Structure of coronavirus main proteinase reveals combination of a chymotrypsin fold with an extra alpha-helical domain

scientific article

Structure of glutamate carboxypeptidase II, a drug target in neuronal damage and prostate cancer

scientific article

Structure of the GTPase and GDI domains of FeoB, the ferrous iron transporter of Legionella pneumophila

scientific article

Structure of the X (ADRP) domain of nsp3 from feline coronavirus

scientific article

Structure-Based Antivirals for Emerging and Neglected Rna Viruses: An Emerging Field for Medicinal Chemistry in Academia ⬇️

scientific article published on July 1, 2010

Structures of DegQ from Legionella pneumophila Define Distinct ON and OFF States

scientific article

Substrate specificity profiling of SARS-CoV-2 Mpro protease provides basis for anti-COVID-19 drug design

scientific article (preprint)

Sugar-mediated lattice contacts in crystals of a plant glycoprotein

scientific article published on 26 September 2002

Synthesis and antiplasmodial activity of a cysteine protease-inhibiting biotinylated aziridine-2,3-dicarboxylate

scientific article

The "SARS-unique domain" (SUD) of SARS coronavirus is an oligo(G)-binding protein

scientific article published on 23 October 2007

The Legionella HtrA homologue DegQ is a self-compartmentizing protease that forms large 12-meric assemblies

scientific article

The PPIase active site of Legionella pneumophila Mip protein is involved in the infection of eukaryotic host cells

scientific article published on January 2003

The SARS-CoV-2 main protease Mpro causes microvascular brain pathology by cleaving NEMO in brain endothelial cells

scientific article

The SARS-Unique Domain (SUD) of SARS Coronavirus Contains Two Macrodomains That Bind G-Quadruplexes

scientific article

The SARS-coronavirus-host interactome: identification of cyclophilins as target for pan-coronavirus inhibitors

scientific article

The SARS‐unique domain (SUD) of SARS‐CoV and SARS‐CoV‐2 interacts with human Paip1 to enhance viral RNA translation

scholarly article by Jian Lei published in April 2021

The Structure of the Zika Virus Protease, NS2B/NS3pro.

scientific article published in January 2018

The active principle of garlic at atomic resolution

scientific article

The capsid binder Vapendavir and the novel protease inhibitor SG85 inhibit enterovirus 71 replication

scientific article

The crystal structure of a Cys25 -> Ala mutant of human procathepsin S elucidates enzyme-prosequence interactions

scientific article published on November 2006

The crystal structures of severe acute respiratory syndrome virus main protease and its complex with an inhibitor ⬇️

scientific article published on October 29, 2003

The enterovirus 3C protease inhibitor SG85 efficiently blocks rhinovirus replication and is not cross-resistant with rupintrivir

scientific article published on 8 June 2015

The evolution of insulin glargine and its continuing contribution to diabetes care

scientific article

The macromolecular complex of ICP and falcipain-2 fromPlasmodium: preparation, crystallization and preliminary X-ray diffraction analysis ⬇️

scientific article published on October 27, 2011

The non-structural protein Nsp10 of mouse hepatitis virus binds zinc ions and nucleic acids

scientific article published on 30 June 2006

The role of protein 3D-structures in the drug discovery process.

scientific article

The structure of a glycoside hydrolase family 81 endo-β-1,3-glucanase.

scientific article

The structure of the unliganded extracellular domain of the interleukin-6 signal transducer gp130 in solution

scientific article published on 30 October 2010

Thiazolidone derivatives as inhibitors of chikungunya virus

scientific article published on 16 October 2014

Third Tofo Advanced Study Week on Emerging and Re-emerging Viruses, 2018

scientific article published on 28 December 2018

Unusual binding mode of an HIV-1 protease inhibitor explains its potency against multi-drug-resistant virus strains

scientific article

Utility of homology models in the drug discovery process.

scientific article

Variable oligomerization modes in coronavirus non-structural protein 9

scientific article

Viral Entry and NS1 as Potential Antiviral Drug Targets.

scientific article published in January 2018

Viral enzymes

scientific article published on 7 November 2006

Virus–host interactomes — antiviral drug discovery ⬇️

scientific article published on October 1, 2012

WaaA of the hyperthermophilic bacterium Aquifex aeolicus is a monofunctional 3-deoxy-D-manno-oct-2-ulosonic acid transferase involved in lipopolysaccharide biosynthesis

scientific article

X-ray Structure of Main Protease of the Novel Coronavirus SARS-CoV-2 Enables Design of α-Ketoamide Inhibitors

scientific article

X-ray screening identifies active site and allosteric inhibitors of SARS-CoV-2 main protease

scientific article

X-ray structure of the SH3 domain of the phosphoinositide 3-kinase p85β subunit

scientific article

Zika virus NS1, a pathogenicity factor with many faces

scientific article published on 27 October 2016

p53 down-regulates SARS coronavirus replication and is targeted by the SARS-unique domain and PLpro via E3 ubiquitin ligase RCHY1

scientific article published on 12 August 2016

pH-dependent conformational flexibility of the SARS-CoV main proteinase (M(pro)) dimer: molecular dynamics simulations and multiple X-ray structure analyses

scientific article published on 23 September 2005

ppGpp analogues inhibit synthetase activity of Rel proteins from Gram-negative and Gram-positive bacteria

scientific article published on 28 April 2010

α-Ketoamides as Broad-Spectrum Inhibitors of Coronavirus and Enterovirus Replication: Structure-Based Design, Synthesis, and Activity Assessment

scientific article published on 24 February 2020

List of works by Rolf Hilgenfeld

3C Protease of Enterovirus 68: Structure-Based Design of Michael Acceptor Inhibitors and Their Broad-Spectrum Antiviral Effects against Picornaviruses

A G-quadruplex-binding macrodomain within the "SARS-unique domain" is essential for the activity of the SARS-coronavirus replication-transcription complex.

A structural view of the inactivation of the SARS coronavirus main proteinase by benzotriazole esters

Accessory proteins of SARS-CoV and other coronaviruses

Acquisition of new protein domains by coronaviruses: analysis of overlapping genes coding for proteins N and 9b in SARS coronavirus

Alpha-ketoamides as broad-spectrum inhibitors of coronavirus and enterovirus replication

An efficient method for the synthesis of peptide aldehyde libraries employed in the discovery of reversible SARS coronavirus main protease (SARS-CoV Mpro) inhibitors

An overview on 2-methyl-2,4-pentanediol in crystallization and in crystals of biological macromolecules

An unfolding/refolding step helps in the crystallization of a poorly soluble protein

Antiviral Activity of Broad-Spectrum and Enterovirus-Specific Inhibitors against Clinical Isolates of Enterovirus D68.

Antiviral strategies to control calicivirus infections

Application of a cell-based protease assay for testing inhibitors of picornavirus 3C proteases

Architecture and regulation of HtrA-family proteins involved in protein quality control and stress response ⬇️

Biochemical and functional analyses of the Mip protein: influence of the N-terminal half and of peptidylprolyl isomerase activity on the virulence of Legionella pneumophila

Catalytic cleavage of HEAT and subsequent covalent binding of the tetralone moiety by the SARS-CoV-2 main protease

Characterization of an Allosteric Pocket in Zika Virus NS2B-NS3 Protease

Chemotherapeutic Effects of Bioassay-Guided Extracts of the American Cockroach, Periplaneta americana ⬇️

Computer-Aided Structure Based Drug Design Approaches for the Discovery of New Anti-CHIKV Agents

Conformational antagonism between opposing active sites in a bifunctional RelA/SpoT homolog modulates (p)ppGpp metabolism during the stringent response [corrected].

Coronavirus main proteinase (3CLpro) structure: basis for design of anti-SARS drugs

Correction: Design of Wide-Spectrum Inhibitors Targeting Coronavirus Main Proteases

Coxsackievirus B3 protease 3C: expression, purification, crystallization and preliminary structural insights

Crystal Structure of the Peroxo-diiron(III) Intermediate of Deoxyhypusine Hydroxylase, an Oxygenase Involved in Hypusination

Crystal structure of SARS-CoV-2 main protease provides a basis for design of improved α-ketoamide inhibitors

Crystal structure of Zika virus NS2B-NS3 protease in complex with a boronate inhibitor

Crystal structure of the C-terminal fragment of NS1 protein from yellow fever virus

Crystal structure of the middle domain of human poly(A)-binding protein-interacting protein 1 ⬇️

Crystal structure of the papain-like protease of MERS coronavirus reveals unusual, potentially druggable active-site features

Crystal structures of the X-domains of a Group-1 and a Group-3 coronavirus reveal that ADP-ribose-binding may not be a conserved property

Crystallization comes of age

Design of wide-spectrum inhibitors targeting coronavirus main proteases

Development and Characterization of New Peptidomimetic Inhibitors of the West Nile Virus NS2B–NS3 Protease ⬇️

Effects of NS2B-NS3 protease and furin inhibition on West Nile and Dengue virus replication

Erratum to: Liberation of SARS-CoV main protease from the viral polyprotein: N-terminal autocleavage does not depend on the mature dimerization mode.

Erratum: Crystal structure of active elongation factor Tu reveals major domain rearrangements

Evidence for a Two-Metal-Ion Mechanism in the Cytidyltransferase KdsB, an Enzyme Involved in Lipopolysaccharide Biosynthesis ⬇️

Exoerythrocytic Plasmodium parasites secrete a cysteine protease inhibitor involved in sporozoite invasion and capable of blocking cell death of host hepatocytes

Extended substrate specificity and first potent irreversible inhibitor/activity-based probe design for Zika virus NS2B-NS3 protease

From SARS to MERS: 10 years of research on highly pathogenic human coronaviruses

From SARS to MERS: crystallographic studies on coronaviral proteases enable antiviral drug design

Functional Characterization of the Cleavage Specificity of the Sapovirus Chymotrypsin-Like Protease

Helix Unfolding/Refolding Characterizes the Functional Dynamics of Staphylococcus aureus Clp Protease ⬇️

Human glutamate carboxypeptidase II inhibition: structures of GCPII in complex with two potent inhibitors, quisqualate and 2-PMPA

Identification of novel falcipain-2 inhibitors as potential antimalarial agents through structure-based virtual screening.

Improved synthesis of rupintrivir

Improving experimental phases for strong reflections prior to density modification

Irreversible inhibitors of the 3C protease of Coxsackie virus through templated assembly of protein-binding fragments

Liberation of SARS-CoV main protease from the viral polyprotein: N-terminal autocleavage does not depend on the mature dimerization mode ⬇️

Lybatides from Lycium barbarum Contain An Unusual Cystine-stapled Helical Peptide Scaffold

Modern methods of drug discovery: an introduction

Molecular mechanisms of severe acute respiratory syndrome (SARS)

Non structural proteins 8 and 9 of human coronavirus 229E.

Nonstructural Proteins 7 and 8 of Feline Coronavirus Form a 2:1 Heterotrimer That Exhibits Primer-Independent RNA Polymerase Activity

Nonstructural proteins of human coronavirus NL63.

Nsp3 of coronaviruses: Structures and functions of a large multi-domain protein

Nucleocapsid protein of SARS coronavirus tightly binds to human cyclophilin A.

Optimization of Protein Crystallization: The OptiCryst Project

Peptide aldehyde inhibitors challenge the substrate specificity of the SARS-coronavirus main protease

Peptide-Boronic Acid Inhibitors of Flaviviral Proteases: Medicinal Chemistry and Structural Biology

PhyloMap: an algorithm for visualizing relationships of large sequence data sets and its application to the influenza A virus genome ⬇️

Picornavirus non-structural proteins as targets for new anti-virals with broad activity

Processing of the SARS-CoV pp1a/ab nsp7-10 region

Processing of the SARS-CoV pp1a/ab nsp7-10 region

Production of coronavirus nonstructural proteins in soluble form for crystallization

Production, crystallization and X-ray diffraction analysis of the protease CT441 from Chlamydia trachomatis

Profiling of flaviviral NS2B-NS3 protease specificity provides a structural basis for the development of selective chemical tools that differentiate Dengue from Zika and West Nile viruses

Purification, characterization, and crystallization of alliinase from garlic

RNA-virus proteases counteracting host innate immunity

Recent Advances in Neuraminidase Inhibitor Development as Anti‐influenza Drugs ⬇️

Recent advances in targeting viral proteases for the discovery of novel antivirals

SARS-CoV-2 Mpro inhibitors and activity-based probes for patient-sample imaging

STD-NMR experiments identify a structural motif with novel second-site activity against West Nile virus NS2B-NS3 protease

Sensitized detection of inhibitory fragments and iterative development of non-peptidic protease inhibitors by dynamic ligation screening

Single-drop optimization of protein crystallization ⬇️

Sometimes intermediates. Do the job!

Structural and functional characterization of Falcipain-2, a hemoglobinase from the malarial parasite Plasmodium falciparum

Structural and mechanistic analysis of the membrane-embedded glycosyltransferase WaaA required for lipopolysaccharide synthesis

Structural and mutational analysis of the interaction between the Middle-East respiratory syndrome coronavirus (MERS-CoV) papain-like protease and human ubiquitin

Structural basis for the regulation of cysteine-protease activity by a new class of protease inhibitors in Plasmodium