List of works - Mario Milani

A Crystallographer’s Perspective on the 2/2Hb Family

A TyrCD1/TrpG8 hydrogen bond network and a TyrB10TyrCD1 covalent link shape the heme distal site of Mycobacterium tuberculosis hemoglobin O.

scientific article

A stereospecific carboxyl esterase from Bacillus coagulans hosting nonlipase activity within a lipase-like fold.

scientific article published on 26 December 2017

An active-like structure in the unphosphorylated StyR response regulator suggests a phosphorylation- dependent allosteric activation mechanism

scientific article

CO sniffing through heme-based sensor proteins

scientific article published in June 2004

Combined in silico and in vitro approaches identified the antipsychotic drug lurasidone and the antiviral drug elbasvir as SARS-CoV2 and HCoV-OC43 inhibitors

scientific article published on 10 March 2021

Crystal Structure of a Novel Conformational State of the Flavivirus NS3 Protein: Implications for Polyprotein Processing and Viral Replication

scientific article

Crystal structure and activity of Kunjin virus NS3 helicase; protease and helicase domain assembly in the full length NS3 protein

scientific article

Crystal structure of YeaZ from Pseudomonas aeruginosa

scientific article

Crystal structure of a methyltransferase from a no-known-vector Flavivirus

scientific article

Cyanide binding to truncated hemoglobins: a crystallographic and kinetic study

scientific article

Delivery of suramin as an antiviral agent through liposomal systems

scientific article

Designing Smac-mimetics as antagonists of XIAP, cIAP1, and cIAP2

scientific article

Dimeric Smac mimetics/IAP inhibitors as in vivo-active pro-apoptotic agents. Part II: Structural and biological characterization

scientific article

Evidence for self-association of the alternative sigma factor σ54

scientific article

FAD-binding site and NADP reactivity in human renalase: a new enzyme involved in blood pressure regulation

scientific article

Ferredoxin-NADP+ reductase from Plasmodium falciparum undergoes NADP+-dependent dimerization and inactivation: functional and crystallographic analysis

scientific article

Flaviviral Helicases: Structure, Function, Inhibition and Dynamics

article

Flaviviral helicase: Insights into the mechanism of action of a motor protein ⬇️

scientific article published on November 19, 2011

Flaviviral methyltransferase/RNA interaction: structural basis for enzyme inhibition

scientific article

Functionalized 2,1-benzothiazine 2,2-dioxides as new inhibitors of Dengue NS5 RNA-dependent RNA polymerase

scientific article published on 11 November 2017

Gelsolin pathogenic Gly167Arg mutation promotes domain-swap dimerization of the protein

scientific article

Glycine Amidinotransferase (GATM), Renal Fanconi Syndrome, and Kidney Failure.

scientific article published on 13 April 2018

Heme-ligand tunneling in group I truncated hemoglobins

scientific article published on 11 March 2004

Hemoprotein time-resolved X-ray crystallography.

scientific article

High-resolution crystal structure of gelsolin domain 2 in complex with the physiological calcium ion

scientific article published on 12 August 2019

Identification of a small molecule that compromises the structural integrity of viroplasms and rotavirus double-layered particles

scientific article published on 15 November 2017

Is renalase a novel player in catecholaminergic signaling? The mystery of the catalytic activity of an intriguing new flavoenzyme

scientific article

Ivermectin is a potent inhibitor of flavivirus replication specifically targeting NS3 helicase activity: new prospects for an old drug

scientific article

Ligand binding to truncated hemoglobin N from Mycobacterium tuberculosis is strongly modulated by the interplay between the distal heme pocket residues and internal water

scientific article published on 2 August 2008

Ligand interactions in the distal heme pocket of Mycobacterium tuberculosis truncated hemoglobin N: roles of TyrB10 and GlnE11 residues

scientific article published in July 2006

Ligand-induced dynamical regulation of NO conversion in Mycobacterium tuberculosis truncated hemoglobin-N

scientific article published on 01 August 2006

Mapping heme-ligand tunnels in group I truncated(2/2) hemoglobins

scientific article published in January 2008

Modulation of Guanylate Cyclase Activating Protein 1 (GCAP1) Dimeric Assembly by Ca2+ or Mg2+: Hints to Understand Protein Activity

scientific article published on 05 October 2020

Molecular basis of a novel renal amyloidosis due to N184K gelsolin variant

scientific article

Mycobacterial truncated hemoglobins: from genes to functions

scientific article published on 29 April 2007

Mycobacterium tuberculosis hemoglobin N displays a protein tunnel suited for O2 diffusion to the heme

scientific article

NF023 binding to XIAP-BIR1: searching drugs for regulation of the NF-κB pathway

scientific article

Naphthalene-sulfonate inhibitors of human norovirus RNA-dependent RNA-polymerase

scientific article

Nitric oxide and Mycobacterium leprae pathogenicity

scientific article

Nitric oxide scavenging by Mycobacterium leprae GlbO involves the formation of the ferric heme-bound peroxynitrite intermediate

scientific article published on 14 November 2005

On the scattering mechanism of the electron impact on surfaces in specular reflection geometry : Nickel (110)

article

PPNDS inhibits murine Norovirus RNA-dependent RNA-polymerase mimicking two RNA stacking bases

scientific article

Peroxynitrite scavenging by ferrous truncated hemoglobin GlbO from Mycobacterium leprae

scientific article published in 2006

Plasmodium falciparum ferredoxin-NADP+ reductase His286 plays a dual role in NADP(H) binding and catalysis

scientific article

Preliminary characterization of (nucleoside-2′-O-)-methyltransferase crystals from Meaban and Yokose flaviviruses

scientific article (publication date: August 2006)

Protein fold and structure in the truncated (2/2) globin family

scientific article published on 29 April 2007

Protein structure in the truncated (2/2) hemoglobin family.

scientific article

Rational design, synthesis and characterization of potent, non-peptidic Smac mimics/XIAP inhibitors as proapoptotic agents for cancer therapy.

scientific article

Recognition of RNA cap in the Wesselsbron virus NS5 methyltransferase domain: implications for RNA-capping mechanisms in Flavivirus

scientific article

Recognition of Smac-mimetic compounds by the BIR domain of cIAP1

scientific article

Retromer stabilization results in neuroprotection in a model of Amyotrophic Lateral Sclerosis

scientific article published on 31 July 2020

Role and inhibition of GLI1 protein in cancer.

scientific article

Scattering mechanism of electrons interacting with surfaces in specular reflection geometry: Graphite

article

Single mutations at the subunit interface modulate copper reactivity in Photobacterium leiognathi Cu,Zn superoxide dismutase

scientific article

Single mutations at the subunit interface modulate copper reactivity in photobacterium leiognathi Cu, Zn superoxide dismutase

Structural Insight into Inhibitor of Apoptosis Proteins Recognition by a Potent Divalent Smac-Mimetic

scientific article

Structural and functional characterization of TgpA, a critical protein for the viability of Pseudomonas aeruginosa

scientific article published on 30 December 2018

Structural bases for heme binding and diatomic ligand recognition in truncated hemoglobins

scientific article

Structural bases of norovirus RNA dependent RNA polymerase inhibition by novel suramin-related compounds

scientific article

Structural bases of the altered catalytic properties of a pathogenic variant of apoptosis inducing factor

scientific article

Structural basis for bivalent Smac-mimetics recognition in the IAP protein family

scientific article

Structural determinants of ligand migration in Mycobacterium tuberculosis truncated hemoglobin O

scientific article published on 01 November 2008

Structural plasticity in the eight-helix fold of a trematode haemoglobin

scientific article

Structure and functionality in flavivirus NS-proteins: perspectives for drug design

scientific article

Structure-based inhibition of Norovirus RNA-dependent RNA polymerases

scientific article

Substrate channeling: Molecular bases

scholarly article by Mario Milani published in July 2003

Targeting flavivirus RNA dependent RNA polymerase through a pyridobenzothiazole inhibitor

scientific article

Targeting the BIR Domains of Inhibitor of Apoptosis (IAP) Proteins in Cancer Treatment

scientific article published on 25 January 2019

Targeting the X-linked inhibitor of apoptosis protein through 4-substituted azabicyclo[5.3.0]alkane smac mimetics. Structure, activity, and recognition principles

scientific article

The 1.6 Å resolution crystal structure of a mutant plastocyanin bearing a 21–25 engineered disulfide bridge

The activator of apoptosis Smac-DIABLO acts as a tetramer in solution

scientific article published on February 2015

The roles of Tyr(CD1) and Trp(G8) in Mycobacterium tuberculosis truncated hemoglobin O in ligand binding and on the heme distal site architecture

scientific article

The truncated hemoglobin from Mycobacterium leprae

scientific article published on 01 June 2002

Truncated hemoglobins and nitric oxide action

scientific article published on October 2003

Truncated hemoglobins: trimming the classical 'three-over-three' globin fold to a minimal size

scientific article published in 2001

Truncated hemoglobins: trimming the classical ‘three-over-three’ globin fold to a minimal size

scientific article published in 2001

Very high resolution structure of a trematode hemoglobin displaying a TyrB10-TyrE7 heme distal residue pair and high oxygen affinity

scientific article

Viscosity-dependent relaxation significantly modulates the kinetics of CO recombination in the truncated hemoglobin TrHbN from Mycobacterium tuberculosis

scientific article published in July 2004

List of works by Mario Milani

A Crystallographer’s Perspective on the 2/2Hb Family

A TyrCD1/TrpG8 hydrogen bond network and a TyrB10TyrCD1 covalent link shape the heme distal site of Mycobacterium tuberculosis hemoglobin O.

A stereospecific carboxyl esterase from Bacillus coagulans hosting nonlipase activity within a lipase-like fold.

An active-like structure in the unphosphorylated StyR response regulator suggests a phosphorylation- dependent allosteric activation mechanism

CO sniffing through heme-based sensor proteins

Combined in silico and in vitro approaches identified the antipsychotic drug lurasidone and the antiviral drug elbasvir as SARS-CoV2 and HCoV-OC43 inhibitors

Crystal Structure of a Novel Conformational State of the Flavivirus NS3 Protein: Implications for Polyprotein Processing and Viral Replication

Crystal structure and activity of Kunjin virus NS3 helicase; protease and helicase domain assembly in the full length NS3 protein

Crystal structure of YeaZ from Pseudomonas aeruginosa

Crystal structure of a methyltransferase from a no-known-vector Flavivirus

Cyanide binding to truncated hemoglobins: a crystallographic and kinetic study

Delivery of suramin as an antiviral agent through liposomal systems

Designing Smac-mimetics as antagonists of XIAP, cIAP1, and cIAP2

Dimeric Smac mimetics/IAP inhibitors as in vivo-active pro-apoptotic agents. Part II: Structural and biological characterization

Evidence for self-association of the alternative sigma factor σ54

FAD-binding site and NADP reactivity in human renalase: a new enzyme involved in blood pressure regulation

Ferredoxin-NADP+ reductase from Plasmodium falciparum undergoes NADP+-dependent dimerization and inactivation: functional and crystallographic analysis

Flaviviral Helicases: Structure, Function, Inhibition and Dynamics

Flaviviral helicase: Insights into the mechanism of action of a motor protein ⬇️

Flaviviral methyltransferase/RNA interaction: structural basis for enzyme inhibition

Functionalized 2,1-benzothiazine 2,2-dioxides as new inhibitors of Dengue NS5 RNA-dependent RNA polymerase

Gelsolin pathogenic Gly167Arg mutation promotes domain-swap dimerization of the protein

Glycine Amidinotransferase (GATM), Renal Fanconi Syndrome, and Kidney Failure.

Heme-ligand tunneling in group I truncated hemoglobins

Hemoprotein time-resolved X-ray crystallography.

High-resolution crystal structure of gelsolin domain 2 in complex with the physiological calcium ion

Identification of a small molecule that compromises the structural integrity of viroplasms and rotavirus double-layered particles

Is renalase a novel player in catecholaminergic signaling? The mystery of the catalytic activity of an intriguing new flavoenzyme

Ivermectin is a potent inhibitor of flavivirus replication specifically targeting NS3 helicase activity: new prospects for an old drug

Ligand binding to truncated hemoglobin N from Mycobacterium tuberculosis is strongly modulated by the interplay between the distal heme pocket residues and internal water

Ligand interactions in the distal heme pocket of Mycobacterium tuberculosis truncated hemoglobin N: roles of TyrB10 and GlnE11 residues

Ligand-induced dynamical regulation of NO conversion in Mycobacterium tuberculosis truncated hemoglobin-N

Mapping heme-ligand tunnels in group I truncated(2/2) hemoglobins

Modulation of Guanylate Cyclase Activating Protein 1 (GCAP1) Dimeric Assembly by Ca2+ or Mg2+: Hints to Understand Protein Activity

Molecular basis of a novel renal amyloidosis due to N184K gelsolin variant

Mycobacterial truncated hemoglobins: from genes to functions

Mycobacterium tuberculosis hemoglobin N displays a protein tunnel suited for O2 diffusion to the heme

NF023 binding to XIAP-BIR1: searching drugs for regulation of the NF-κB pathway

Naphthalene-sulfonate inhibitors of human norovirus RNA-dependent RNA-polymerase

Nitric oxide and Mycobacterium leprae pathogenicity

Nitric oxide scavenging by Mycobacterium leprae GlbO involves the formation of the ferric heme-bound peroxynitrite intermediate

On the scattering mechanism of the electron impact on surfaces in specular reflection geometry : Nickel (110)

PPNDS inhibits murine Norovirus RNA-dependent RNA-polymerase mimicking two RNA stacking bases

Peroxynitrite scavenging by ferrous truncated hemoglobin GlbO from Mycobacterium leprae

Plasmodium falciparum ferredoxin-NADP+ reductase His286 plays a dual role in NADP(H) binding and catalysis

Preliminary characterization of (nucleoside-2′-O-)-methyltransferase crystals from Meaban and Yokose flaviviruses

Protein fold and structure in the truncated (2/2) globin family

Protein structure in the truncated (2/2) hemoglobin family.

Rational design, synthesis and characterization of potent, non-peptidic Smac mimics/XIAP inhibitors as proapoptotic agents for cancer therapy.

Recognition of RNA cap in the Wesselsbron virus NS5 methyltransferase domain: implications for RNA-capping mechanisms in Flavivirus

Recognition of Smac-mimetic compounds by the BIR domain of cIAP1

Retromer stabilization results in neuroprotection in a model of Amyotrophic Lateral Sclerosis

Role and inhibition of GLI1 protein in cancer.

Scattering mechanism of electrons interacting with surfaces in specular reflection geometry: Graphite

Single mutations at the subunit interface modulate copper reactivity in Photobacterium leiognathi Cu,Zn superoxide dismutase

Single mutations at the subunit interface modulate copper reactivity in photobacterium leiognathi Cu, Zn superoxide dismutase

Structural Insight into Inhibitor of Apoptosis Proteins Recognition by a Potent Divalent Smac-Mimetic

Structural and functional characterization of TgpA, a critical protein for the viability of Pseudomonas aeruginosa

Structural bases for heme binding and diatomic ligand recognition in truncated hemoglobins

Structural bases of norovirus RNA dependent RNA polymerase inhibition by novel suramin-related compounds

Structural bases of the altered catalytic properties of a pathogenic variant of apoptosis inducing factor

Structural basis for bivalent Smac-mimetics recognition in the IAP protein family

Structural determinants of ligand migration in Mycobacterium tuberculosis truncated hemoglobin O

Structural plasticity in the eight-helix fold of a trematode haemoglobin

Structure and functionality in flavivirus NS-proteins: perspectives for drug design

Structure-based inhibition of Norovirus RNA-dependent RNA polymerases

Substrate channeling: Molecular bases

Targeting flavivirus RNA dependent RNA polymerase through a pyridobenzothiazole inhibitor

Targeting the BIR Domains of Inhibitor of Apoptosis (IAP) Proteins in Cancer Treatment

Targeting the X-linked inhibitor of apoptosis protein through 4-substituted azabicyclo[5.3.0]alkane smac mimetics. Structure, activity, and recognition principles

The 1.6 Å resolution crystal structure of a mutant plastocyanin bearing a 21–25 engineered disulfide bridge

The activator of apoptosis Smac-DIABLO acts as a tetramer in solution

The roles of Tyr(CD1) and Trp(G8) in Mycobacterium tuberculosis truncated hemoglobin O in ligand binding and on the heme distal site architecture

The truncated hemoglobin from Mycobacterium leprae

Truncated hemoglobins and nitric oxide action

Truncated hemoglobins: trimming the classical 'three-over-three' globin fold to a minimal size

Truncated hemoglobins: trimming the classical ‘three-over-three’ globin fold to a minimal size

Very high resolution structure of a trematode hemoglobin displaying a TyrB10-TyrE7 heme distal residue pair and high oxygen affinity

Viscosity-dependent relaxation significantly modulates the kinetics of CO recombination in the truncated hemoglobin TrHbN from Mycobacterium tuberculosis