List of works - Ralf Ficner

A C-terminal phosphatase module conserved in vertebrate CMP-sialic acid synthetases provides a tetramerization interface for the physiologically active enzyme

scientific article

Atg21 organizes Atg8 lipidation at the contact of the vacuole with the phagophore

scientific article published on 09 June 2020

Biallelic mutations in nucleoporin NUP88 cause lethal fetal akinesia deformation sequence

scientific article published on 13 December 2018

Complex structure of a bacterial class 2 histone deacetylase homologue with a trifluoromethylketone inhibitor

scientific article

Crystal Structure of the Borna Disease Virus Nucleoprotein ⬇️

scientific article published on October 1, 2003

Crystal Structure of the RNA Recognition Motif of Yeast Translation Initiation Factor eIF3b Reveals Differences to Human eIF3b ⬇️

scientific article published on September 16, 2010

Crystal Structures of Physcomitrella patens AOC1 and AOC2: Insights into the Enzyme Mechanism and Differences in Substrate Specificity

scientific article

Crystal structure analysis of DNA uridine endonuclease Mth212 bound to DNA

scientific article

Crystal structure analysis of a fatty acid double-bond hydratase from Lactobacillus acidophilus

scientific article

Crystal structure of a 4-thiouridine synthetase-RNA complex reveals specificity of tRNA U8 modification

scientific article

Crystal structure of a complex between human spliceosomal cyclophilin H and a U4/U6 snRNP-60K peptide

scientific article

Crystal structure of an intramolecular chaperone mediating triple-beta-helix folding

scientific article

Crystal structure of human CRM1, covalently modified by 2-mercaptoethanol on Cys528, in complex with RanGTP

scientific article published on 03 March 2021

Crystal structure of the RRM domain of poly(A)-specific ribonuclease reveals a novel m(7)G-cap-binding mode

scientific article

Crystal structure of the nuclear export receptor CRM1 in complex with Snurportin1 and RanGTP

scientific article

Crystal structures of the c-di-AMP-synthesizing enzyme CdaA

scientific article published on 22 May 2019

Crystal structures of the novel cytosolic 5'-nucleotidase IIIB explain its preference for m7GMP

scientific article

Crystal structures of two tetrameric β-carbonic anhydrases from the filamentous ascomycete Sordaria macrospora

scientific article

De novo sulfur SAD phasing of the lysosomal 66.3 kDa protein from mouse

scientific article

Impact of the crystallization condition on importin-β conformation

scientific article

Initial insight into the function of the lysosomal 66.3 kDa protein from mouse by means of X-ray crystallography

scientific article

Mapping protein interactions in the active TOM-TIM23 supercomplex

scientific article published on 29 September 2021

NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1

scientific article

Observation of a stable carbene at the active site of a thiamin enzyme

scientific article

Phenylalanine-containing hydroxamic acids as selective inhibitors of class IIb histone deacetylases (HDACs)

scientific article

Structural Basis for the Broad Substrate Range of the UDP-Sugar Pyrophosphorylase from Leishmania major ⬇️

scientific article published on November 10, 2010

Structural Basis of Targeting the Exportin CRM1 in Cancer

scientific article

Structural analysis of the C-terminal domain of the spliceosomal helicase Prp22

scientific article

Structural analysis of the intrinsically disordered splicing factor Spp2 and its binding to the DEAH-box ATPase Prp2

scientific article published on 23 January 2020

Structural and Functional Characterization of CRM1-Nup214 Interactions Reveals Multiple FG-Binding Sites Involved in Nuclear Export

scientific article

Structural and functional analysis of the human spliceosomal DEAD-box helicase Prp28

scientific article

Structural basis for RanGTP independent entry of spliceosomal U snRNPs into the nucleus

scientific article

Structural basis for cooperativity of CRM1 export complex formation

scientific article

Structural basis for m3G-cap-mediated nuclear import of spliceosomal UsnRNPs by snurportin1

scientific article

Structural basis for m7G-cap hypermethylation of small nuclear, small nucleolar and telomerase RNA by the dimethyltransferase TGS1

scientific article

Structural basis for the bifunctionality of the U5 snRNP 52K protein (CD2BP2)

scientific article

Structural basis for the recognition and cleavage of polysialic acid by the bacteriophage K1F tailspike protein EndoNF

scientific article

Structural insights into the mechanism of the DEAH-box RNA helicase Prp43

scientific article published on 16 January 2017

Structural insights into the stimulation of S. pombe Dnmt2 catalytic efficiency by the tRNA nucleoside queuosine.

scientific article published on 11 June 2018

Structural integrity of the PCI domain of eIF3a/TIF32 is required for mRNA recruitment to the 43S pre-initiation complexes

scientific article

Structural model of the M7G46 Methyltransferase TrmB in complex with tRNA

scientific article published on 19 May 2021

Structure analysis of Entamoeba histolytica enolase

scientific article

Structure analysis of endosialidase NF at 0.98 A resolution

scientific article

Structure analysis of the conserved methyltransferase domain of human trimethylguanosine synthase TGS1

scientific article

Structure of the E. coli ribosome-EF-Tu complex at <3 Å resolution by Cs-corrected cryo-EM

scientific article

Sub-ångström-resolution crystallography reveals physical distortions that enhance reactivity of a covalent enzymatic intermediate

scientific article published in September 2013

The Velvet Family of Fungal Regulators Contains a DNA-Binding Domain Structurally Similar to NF-κB

scientific article

The crystal structure of the C₂A domain of otoferlin reveals an unconventional top loop region

scientific article

The structure of Prp2 bound to RNA and ADP-BeF<sub>3</sub><sup>-</sup> reveals structural features important for RNA unwinding by DEAH-box ATPases

scientific article published on 30 March 2021

Translation initiation factor eIF3b contains a nine-bladed β-propeller and interacts with the 40S ribosomal subunit

scientific article

Tuning and Switching Enantioselectivity of Asymmetric Carboligation in an Enzyme through Mutational Analysis of a Single Hot Spot

scientific article

Twisted Schiff base intermediates and substrate locale revise transaldolase mechanism

scientific article

Two protein-protein interaction sites on the spliceosome-associated human cyclophilin CypH

scientific article

Unexpected tautomeric equilibria of the carbanion-enamine intermediate in pyruvate oxidase highlight unrecognized chemical versatility of thiamin

scientific article

eIF5B employs a novel domain release mechanism to catalyze ribosomal subunit joining

scientific article

List of works by Ralf Ficner

A C-terminal phosphatase module conserved in vertebrate CMP-sialic acid synthetases provides a tetramerization interface for the physiologically active enzyme

Atg21 organizes Atg8 lipidation at the contact of the vacuole with the phagophore

Biallelic mutations in nucleoporin NUP88 cause lethal fetal akinesia deformation sequence

Complex structure of a bacterial class 2 histone deacetylase homologue with a trifluoromethylketone inhibitor

Crystal Structure of the Borna Disease Virus Nucleoprotein ⬇️

Crystal Structure of the RNA Recognition Motif of Yeast Translation Initiation Factor eIF3b Reveals Differences to Human eIF3b ⬇️

Crystal Structures of Physcomitrella patens AOC1 and AOC2: Insights into the Enzyme Mechanism and Differences in Substrate Specificity

Crystal structure analysis of DNA uridine endonuclease Mth212 bound to DNA

Crystal structure analysis of a fatty acid double-bond hydratase from Lactobacillus acidophilus

Crystal structure of a 4-thiouridine synthetase-RNA complex reveals specificity of tRNA U8 modification

Crystal structure of a complex between human spliceosomal cyclophilin H and a U4/U6 snRNP-60K peptide

Crystal structure of an intramolecular chaperone mediating triple-beta-helix folding

Crystal structure of human CRM1, covalently modified by 2-mercaptoethanol on Cys528, in complex with RanGTP

Crystal structure of the RRM domain of poly(A)-specific ribonuclease reveals a novel m(7)G-cap-binding mode

Crystal structure of the nuclear export receptor CRM1 in complex with Snurportin1 and RanGTP

Crystal structures of the c-di-AMP-synthesizing enzyme CdaA

Crystal structures of the novel cytosolic 5'-nucleotidase IIIB explain its preference for m7GMP

Crystal structures of two tetrameric β-carbonic anhydrases from the filamentous ascomycete Sordaria macrospora

De novo sulfur SAD phasing of the lysosomal 66.3 kDa protein from mouse

Impact of the crystallization condition on importin-β conformation

Initial insight into the function of the lysosomal 66.3 kDa protein from mouse by means of X-ray crystallography

Mapping protein interactions in the active TOM-TIM23 supercomplex

NES consensus redefined by structures of PKI-type and Rev-type nuclear export signals bound to CRM1

Observation of a stable carbene at the active site of a thiamin enzyme

Phenylalanine-containing hydroxamic acids as selective inhibitors of class IIb histone deacetylases (HDACs)

Structural Basis for the Broad Substrate Range of the UDP-Sugar Pyrophosphorylase from Leishmania major ⬇️

Structural Basis of Targeting the Exportin CRM1 in Cancer

Structural analysis of the C-terminal domain of the spliceosomal helicase Prp22

Structural analysis of the intrinsically disordered splicing factor Spp2 and its binding to the DEAH-box ATPase Prp2

Structural and Functional Characterization of CRM1-Nup214 Interactions Reveals Multiple FG-Binding Sites Involved in Nuclear Export

Structural and functional analysis of the human spliceosomal DEAD-box helicase Prp28

Structural basis for RanGTP independent entry of spliceosomal U snRNPs into the nucleus

Structural basis for cooperativity of CRM1 export complex formation

Structural basis for m3G-cap-mediated nuclear import of spliceosomal UsnRNPs by snurportin1

Structural basis for m7G-cap hypermethylation of small nuclear, small nucleolar and telomerase RNA by the dimethyltransferase TGS1

Structural basis for the bifunctionality of the U5 snRNP 52K protein (CD2BP2)

Structural basis for the recognition and cleavage of polysialic acid by the bacteriophage K1F tailspike protein EndoNF

Structural insights into the mechanism of the DEAH-box RNA helicase Prp43

Structural insights into the stimulation of S. pombe Dnmt2 catalytic efficiency by the tRNA nucleoside queuosine.

Structural integrity of the PCI domain of eIF3a/TIF32 is required for mRNA recruitment to the 43S pre-initiation complexes

Structural model of the M7G46 Methyltransferase TrmB in complex with tRNA

Structure analysis of Entamoeba histolytica enolase

Structure analysis of endosialidase NF at 0.98 A resolution

Structure analysis of the conserved methyltransferase domain of human trimethylguanosine synthase TGS1

Structure of the E. coli ribosome-EF-Tu complex at <3 Å resolution by Cs-corrected cryo-EM

Sub-ångström-resolution crystallography reveals physical distortions that enhance reactivity of a covalent enzymatic intermediate

The Velvet Family of Fungal Regulators Contains a DNA-Binding Domain Structurally Similar to NF-κB

The crystal structure of the C₂A domain of otoferlin reveals an unconventional top loop region

The structure of Prp2 bound to RNA and ADP-BeF<sub>3</sub><sup>-</sup> reveals structural features important for RNA unwinding by DEAH-box ATPases

Translation initiation factor eIF3b contains a nine-bladed β-propeller and interacts with the 40S ribosomal subunit

Tuning and Switching Enantioselectivity of Asymmetric Carboligation in an Enzyme through Mutational Analysis of a Single Hot Spot

Twisted Schiff base intermediates and substrate locale revise transaldolase mechanism

Two protein-protein interaction sites on the spliceosome-associated human cyclophilin CypH

Unexpected tautomeric equilibria of the carbanion-enamine intermediate in pyruvate oxidase highlight unrecognized chemical versatility of thiamin

eIF5B employs a novel domain release mechanism to catalyze ribosomal subunit joining