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List of works by Nigel Scrutton

(1)H, (15)N and (13)C backbone resonance assignments of pentaerythritol tetranitrate reductase from Enterobacter cloacae PB2.

scientific article published on 22 November 2017

1H, 15N, 13C backbone resonance assignments of human soluble catechol O-methyltransferase in complex with S-adenosyl-L-methionine and 3,5-dinitrocatechol

scientific article published on 15 December 2016

A 'Plug and Play' Platform for the Production of Diverse Monoterpene Hydrocarbon Scaffolds in Escherichia coli. ⬇️

scientific article published in June 2016

A Toolbox for Diverse Oxyfunctionalisation of Monoterpenes

scientific article published in Scientific Reports

A Twin-track Approach Has Optimized Proton and Hydride Transfer by Dynamically Coupled Tunneling during the Evolution of Protochlorophyllide Oxidoreductase ⬇️

scientific article published on February 11, 2011

A brain-permeable inhibitor of the neurodegenerative disease target kynurenine 3-monooxygenase prevents accumulation of neurotoxic metabolites

scientific article published on 24 July 2019

A conformational sampling model for radical catalysis in pyridoxal phosphate- and cobalamin-dependent enzymes.

scientific article

A microbial platform for renewable propane synthesis based on a fermentative butanol pathway.

scientific article published on 10 April 2015

A new conceptual framework for enzyme catalysis

scientific article (publication date: July 2002)

A new conceptual framework for enzyme catalysis. Hydrogen tunnelling coupled to enzyme dynamics in flavoprotein and quinoprotein enzymes

scientific article (publication date: July 2002)

A perspective on conformational control of electron transfer in nitric oxide synthases

scientific article published on 9 September 2016

A site-saturated mutagenesis study of pentaerythritol tetranitrate reductase reveals that residues 181 and 184 influence ligand binding, stereochemistry and reactivity

scientific article

A stable tyrosyl radical in monoamine oxidase A.

scientific article published on 18 November 2004

A surprising observation that oxygen can affect the product enantiopurity of an enzyme-catalysed reaction

article

Ab Initio QM/MM Modeling of the Rate-Limiting Proton Transfer Step in the Deamination of Tryptamine by Aromatic Amine Dehydrogenase

scientific article

Active Intermediates in Copper Nitrite Reductase Reactions Probed by a Cryotrapping-Electron Paramagnetic Resonance Approach

scientific article published on 30 April 2020

Active site modifications in pentaerythritol tetranitrate reductase can lead to improved product enantiopurity, decreased by-product formation and altered stereochemical outcome in reactions with α,β-unsaturated nitroolefins

article

Advantages of brain penetrating inhibitors of kynurenine-3-monooxygenase for treatment of neurodegenerative diseases

scientific article published on 01 December 2020

Alternative metabolic pathways and strategies to high-titre terpenoid production in <i>Escherichia coli</i>

scientific article published in 2022

An Internal Reaction Chamber in Dimethylglycine Oxidase Provides Efficient Protection from Exposure to Toxic Formaldehyde

scientific article

An automated Design-Build-Test-Learn pipeline for enhanced microbial production of fine chemicals

scientific article published on 08 June 2018

An automated pipeline for the screening of diverse monoterpene synthase libraries

An oxidative N-demethylase reveals PAS transition from ubiquitous sensor to enzyme

scientific article published on 16 November 2016

Analysis of Classical and Quantum Paths for Deprotonation of Methylamine by Methylamine Dehydrogenase

scientific article published on 01 August 2007

Are Environmentally Coupled Enzymatic Hydrogen Tunneling Reactions Influenced by Changes in Solution Viscosity?

scientific article published on 01 January 2008

Are the catalytic properties of enzymes from piezophilic organisms pressure adapted?

scientific article

Assignment of the vibrational spectra of enzyme-bound tryptophan tryptophyl quinones using a combined QM/MM approach

scientific article published on January 2010

Asymmetric Reduction of Activated Alkenes by Pentaerythritol Tetranitrate Reductase: Specificity and Control of Stereochemical Outcome by Reaction Optimisation.

scientific article published on November 2009

Atomic description of an enzyme reaction dominated by proton tunneling

scientific article

Atomic level insight into the oxidative half-reaction of aromatic amine dehydrogenase

scientific article published on 27 September 2006

Atomic resolution structures and solution behavior of enzyme-substrate complexes of Enterobacter cloacae PB2 pentaerythritol tetranitrate reductase. Multiple conformational states and implications for the mechanism of nitroaromatic explosive degrada

scientific article

Atomistic insight into the origin of the temperature-dependence of kinetic isotope effects and H-tunnelling in enzyme systems is revealed through combined experimental studies and biomolecular simulation.

scientific article published on February 2008

Barrier compression and its contribution to both classical and quantum mechanical aspects of enzyme catalysis.

scientific article published on January 2010

Barrier compression enhances an enzymatic hydrogen-transfer reaction.

scientific article published in January 2009

Better than Nature: Nicotinamide Biomimetics That Outperform Natural Coenzymes

scientific article published on 3 January 2016

Bio-derived production of cinnamyl alcohol via a three step biocatalytic cascade and metabolic engineering

scientific article published on 05 January 2018

Biocatalysis with thermostable enzymes: structure and properties of a thermophilic 'ene'-reductase related to old yellow enzyme

scientific article

Biocatalytic Asymmetric Alkene Reduction: Crystal Structure and Characterization of a Double Bond Reductase from Nicotiana tabacum

scientific article

Biocatalytic Routes to Lactone Monomers for Polymer Production

scientific article published on 13 March 2018

Bipartite recognition and conformational sampling mechanisms for hydride transfer from nicotinamide coenzyme to FMN in pentaerythritol tetranitrate reductase

scientific article published on 31 July 2009

C3 and C6 Modification-Specific OYE Biotransformations of Synthetic Carvones and Sequential BVMO Chemoenzymatic Synthesis of Chiral Caprolactones

scientific article published on 15 January 2019

Carbon monoxide poisoning is prevented by the energy costs of conformational changes in gas-binding haemproteins

scientific article

Catalysis by the isolated tryptophan tryptophylquinone-containing subunit of aromatic amine dehydrogenase is distinct from native enzyme and synthetic model compounds and allows further probing of TTQ mechanism

scientific article published on 5 December 2007

Catalytic mechanism of cofactor-free dioxygenases and how they circumvent spin-forbidden oxygenation of their substrates.

scientific article published on 4 June 2015

Channelling and formation of 'active' formaldehyde in dimethylglycine oxidase

scientific article

Chapter 10. Computational Simulations of Tunnelling Reactions in Enzymes

Characterization of coenzyme binding and selectivity determinants in Mycobacterium tuberculosis flavoprotein reductase A: analysis of Arg(199) and Arg(200) mutants at the NADP(H) 2'-phosphate binding site.

scientific article published in January 2009

Chemoenzymatic Synthesis of the Intermediates in the Peppermint Monoterpenoid Biosynthetic Pathway

scientific article published on 06 July 2018

Cobalamin uptake and reactivation occurs through specific protein interactions in the methionine synthase-methionine synthase reductase complex

scientific article published on 20 February 2009

Comprehensive analysis of the green-to-blue photoconversion of full-length Cyanobacteriochrome Tlr0924

scientific article

Computational studies of enzyme mechanism: linking theory with experiment in the analysis of enzymic H-tunnelling.

scientific article published on 22 August 2006

Conformational and Thermodynamic Control of Electron Transfer in Neuronal Nitric Oxide Synthase†

scientific article published on 06 April 2007

Conformational change in cytochrome P450 reductase adsorbed at a Au(110)—phosphate buffer interface induced by interaction with nicotinamide adenine dinucleotide phosphate

scientific article published on 13 August 2014

Conformational change induced by electron transfer in a monolayer of cytochrome P450 reductase adsorbed at the Au(110)-phosphate buffer interface.

scientific article

Conformational changes in the catalytic cycle of protochlorophyllide oxidoreductase: what lessons can be learnt from dihydrofolate reductase?

scientific article published on April 2009

Conformational dynamics of the cytochrome P450 BM3/N-palmitoylglycine complex: the proposed "proximal-distal" transition probed by temperature-jump spectroscopy.

scientific article published on 16 June 2007

Conformational events during ternary enzyme-substrate complex formation are rate limiting in the catalytic cycle of the light-driven enzyme protochlorophyllide oxidoreductase.

scientific article

Continuous two-phase flow miniaturised bioreactor for monitoring anaerobic biocatalysis by pentaerythritol tetranitrate reductase

scientific article published on 7 June 2010

Continuous wave photolysis magnetic field effect investigations with free and protein-bound alkylcobalamins

scientific article published in December 2009

Convergence of theory and experiment on the role of preorganization, quantum tunneling and enzyme motions into flavoenzyme-catalyzed hydride transfer.

scientific article

Conversion of alcohols to enantiopure amines through dual-enzyme hydrogen-borrowing cascades

scientific article (publication date: 25 September 2015)

Correction of pre-steady-state KIEs for isotopic impurities and the consequences of kinetic isotope fractionation

scientific article

Correlating Calmodulin Landscapes with Chemical Catalysis in Neuronal Nitric Oxide Synthase using Time-Resolved FRET and a 5-Deazaflavin Thermodynamic Trap

scientific article

Coupled motions direct electrons along human microsomal P450 Chains

scientific article

Co‐production of biofuel, bioplastics and biochemicals during extended fermentation of <i>Halomonas bluephagenesis</i>

scientific article published in 2022

Cross-Species Analysis of Protein Dynamics Associated with Hydride and Proton Transfer in the Catalytic Cycle of the Light-Driven Enzyme Protochlorophyllide Oxidoreductase.

scientific article published on 25 January 2016

Cryogenic and laser photoexcitation studies identify multiple roles for active site residues in the light-driven enzyme protochlorophyllide oxidoreductase

scientific article

Cryptochrome-dependent magnetic field effect on seizure response in Drosophila larvae

scientific article

Crystal structure and solution characterization of the activation domain of human methionine synthase

scientific article

Crystal structure of [1,1'''-bis-(pyrimidin-2-yl)-4,4':2',2'':4'',4'''-quaterpyridine-1,1'''-diium-κ(2) N (1'),N (1'')]bis-[2-(pyridin-2-yl)phenyl-κ(2) N,C (1)]iridium(III) tris-(hexa-fluorido-phosphate) aceto-nitrile tris-olvate

scientific article published on 4 July 2015

Crystal structure of a soluble form of human CD73 with ecto-5'-nucleotidase activity

scientific article

Crystal structure of bacterial morphinone reductase and properties of the C191A mutant enzyme

scientific article

Crystallization and preliminary X-ray diffraction analysis of a flavoenzyme amine dehydrogenase/oxidase from Pyrococcus furiosus DSM 3638.

scientific article published on 8 July 2005

DNA binding suppresses human AIF-M2 activity and provides a connection between redox chemistry, reactive oxygen species, and apoptosis

scientific article (publication date: 12 October 2007)

Deep tunneling dominates the biologically important hydride transfer reaction from NADH to FMN in morphinone reductase

scientific article published on 10 May 2008

Demonstration of proton-coupled electron transfer in the copper-containing nitrite reductases

scientific article published on 7 July 2009

Determination of the redox potentials and electron transfer properties of the FAD- and FMN-binding domains of the human oxidoreductase NR1

scientific article

Direct Evidence of an Excited-State Triplet Species upon Photoactivation of the Chlorophyll Precursor Protochlorophyllide.

scientific article published on 28 February 2017

Direct analysis of donor-acceptor distance and relationship to isotope effects and the force constant for barrier compression in enzymatic H-tunneling reactions

scientific article published on August 2010

Discovery, Characterisation, Engineering and Applications of Ene Reductases for Industrial Biocatalysis.

scientific article published on 20 March 2018

Does the pressure dependence of kinetic isotope effects report usefully on dynamics in enzyme H-transfer reactions?

scientific article

Donor-Acceptor Distance Sampling Enhances the Performance of "Better than Nature" Nicotinamide Coenzyme Biomimetics

scientific article

Driving force analysis of proton tunnelling across a reactivity series for an enzyme-substrate complex.

scientific article

Dual role of the active site 'lid' regions of protochlorophyllide oxidoreductase in photocatalysis and plant development

scientific article published on 31 August 2020

Dynamic, electrostatic model for the generation and control of high-energy radical intermediates by a coenzyme B₁₂-dependent enzyme.

scientific article published on 19 August 2013

Dynamics driving function: new insights from electron transferring flavoproteins and partner complexes.

scientific article published on 17 October 2007

ELDOR Spectroscopy Reveals that Energy Landscapes in Human Methionine Synthase Reductase are Extensively Remodelled Following Ligand and Partner Protein Binding

scientific article published on 25 February 2011

Effects of multiple ligand binding on kinetic isotope effects in PQQ-dependent methanol dehydrogenase

scientific article published in April 2003

Electrical circuitry in biology: emerging principles from protein structure.

scientific article

Electron transfer and conformational change in complexes of trimethylamine dehydrogenase and electron transferring flavoprotein.

scientific article

Electron transfer in flavocytochrome P450 BM3: kinetics of flavin reduction and oxidation, the role of cysteine 999, and relationships with mammalian cytochrome P450 reductase

scientific article published in September 2003

Electron transfer in human methionine synthase reductase studied by stopped-flow spectrophotometry

scientific article

Energy landscapes and catalysis in nitric-oxide synthase

scientific article published on 7 March 2014

Engineering nature for gaseous hydrocarbon production

scientific article published on 13 November 2020

Engineering proximal vs. distal heme-NO coordination via dinitrosyl dynamics: implications for NO sensor design.

scientific article

Engineering the "Missing Link" in Biosynthetic (-)-Menthol Production: Bacterial Isopulegone Isomerase.

scientific article

Enzymatic Menthol Production: One-Pot Approach Using Engineered Escherichia coli

scientific article published on 27 May 2015

Enzymatic Single-Molecule Kinetic Isotope Effects

scientific article published on 19 February 2013

Enzyme mechanisms: fast reaction and computational approaches

scientific article published on April 2009

Equatorial Active Site Compaction and Electrostatic Reorganization in Catechol--methyltransferase

Evidence to support the hypothesis that promoting vibrations enhance the rate of an enzyme catalyzed H-tunneling reaction

scientific article

Examining the importance of dynamics, barrier compression and hydrogen tunnelling in enzyme catalysed reactions

article published in 2011

Excited state dynamics and catalytic mechanism of the light-driven enzyme protochlorophyllide oxidoreductase

scientific article published on 14 March 2012

Excited state dynamics can be used to probe donor-acceptor distances for H-tunneling reactions catalyzed by flavoproteins

scientific article published on December 2013

Excited-State Properties of Protochlorophyllide Analogues and Implications for Light-Driven Synthesis of Chlorophyll.

scientific article

Excited-state charge separation in the photochemical mechanism of the light-driven enzyme protochlorophyllide oxidoreductase

scientific article published on 8 December 2014

Experiment and Simulation Reveal How Mutations in Functional Plasticity Regions Guide Plant Monoterpene Synthase Product Outcome

article

Exploiting Single Domain Antibodies as Regulatory Parts to Modulate Monoterpenoid Production in E. coli

scientific article published on 14 September 2020

Exploring novel bacterial terpene synthases

scientific article published on 30 April 2020

Extensive conformational sampling in a ternary electron transfer complex

scientific article (publication date: March 2003)

Extensive domain motion and electron transfer in the human electron transferring flavoprotein.medium chain Acyl-CoA dehydrogenase complex.

scientific article

Fast protein motions are coupled to enzyme H-transfer reactions

scientific article published on 11 February 2013

Flavin radicals, conformational sampling and robust design principles in interprotein electron transfer: the trimethylamine dehydrogenase-electron-transferring flavoprotein complex

scientific article

Focused directed evolution of pentaerythritol tetranitrate reductase by using automated anaerobic kinetic screening of site-saturated libraries

scientific article

From Bugs to Bioplastics: Total (+)-Dihydrocarvide Biosynthesis by Engineered Escherichia coli

scientific article published on 21 January 2019

Gating mechanisms for biological electron transfer: integrating structure with biophysics reveals the nature of redox control in cytochrome P450 reductase and copper-dependent nitrite reductase.

scientific article published on 14 July 2011

Genome Editing for the Production of Natural Products in Escherichia coli

article

Glutamate 338 is an electrostatic facilitator of C-Co bond breakage in a dynamic/electrostatic model of catalysis by ornithine aminomutase

scientific article published on 12 February 2015

Good vibrations in enzyme-catalysed reactions

scientific article

Graphene-aramid nanocomposite fibres via superacid co-processing

scientific article published on 01 September 2019

H-transfers in Photosystem II: what can we learn from recent lessons in the enzyme community?

scientific article published on 03 September 2008

H-tunneling in the multiple H-transfers of the catalytic cycle of morphinone reductase and in the reductive half-reaction of the homologous pentaerythritol tetranitrate reductase.

scientific article published on 26 August 2003

Highly multiplexed, fast and accurate nanopore sequencing for verification of synthetic DNA constructs and sequence libraries

scientific article published on 29 October 2019

How does pressure affect barrier compression and isotope effects in an enzymatic hydrogen tunneling reaction?

scientific article published on 25 January 2011

Human cryptochrome-1 confers light independent biological activity in transgenic Drosophila correlated with flavin radical stability.

scientific article

Hydrogen tunneling in quinoproteins.

scientific article published in August 2004

Hydrogen tunnelling in enzyme-catalysed H-transfer reactions: flavoprotein and quinoprotein systems.

scientific article published on August 2006

Identification of Covalent Flavoproteins and Analysis of the Covalent Link

article

Impact of residues remote from the catalytic centre on enzyme catalysis of copper nitrite reductase

scientific article

In conversation with Nigel Scrutton

scientific article published on 29 December 2020

In silico design and automated learning to boost next-generation smart biomanufacturing

scientific article published on 17 October 2020

Incorporation of hydrostatic pressure into models of hydrogen tunneling highlights a role for pressure-modulated promoting vibrations

scientific article published on 22 August 2008

Inside Cover: Focused Directed Evolution of Pentaerythritol Tetranitrate Reductase by Using Automated Anaerobic Kinetic Screening of Site-Saturated Libraries (ChemBioChem 17/2010)

scientific article published in 2010

Insights into the H<sub>2</sub> O<sub>2</sub> -driven catalytic mechanism of fungal lytic polysaccharide monooxygenases

scientific article published on 23 December 2020

Inter-flavin electron transfer in cytochrome P450 reductase - effects of solvent and pH identify hidden complexity in mechanism.

scientific article

Internal electron transfer in multi-site redox enzymes is accessed by laser excitation of thiouredopyrene-3,6,8-trisulfonate (TUPS).

scientific article

Introduction. Quantum catalysis in enzymes: beyond the transition state theory paradigm

scientific article published on 12 July 2006

Is There a Dynamic Protein Contribution to the Substrate Trigger in Coenzyme B12-Dependent Ethanolamine Ammonia Lyase?

scientific article published on 22 September 2011

Isotope effects reveal that para-substituted benzylamines are poor reactivity probes of the quinoprotein mechanism for aromatic amine dehydrogenase

scientific article

Isotopically labeled flavoenzymes and their uses in probing reaction mechanisms

scientific article published on 29 March 2019

Kinetic and spectroscopic probes of motions and catalysis in the cytochrome P450 reductase family of enzymes

scientific article published on 09 January 2012

Kinetic and structural basis of reactivity of pentaerythritol tetranitrate reductase with NADPH, 2-cyclohexenone, nitroesters, and nitroaromatic explosives

scientific article

Kinetic and thermodynamic characterization of the common polymorphic variants of human methionine synthase reductase

scientific article (publication date: 24 February 2004)

Kinetic isotope effects and ligand binding in PQQ-dependent methanol dehydrogenase.

scientific article published on May 2005

Kinetic, spectroscopic and thermodynamic characterization of the Mycobacterium tuberculosis adrenodoxin reductase homologue FprA

scientific journal article

Large-Scale Domain Conformational Change Is Coupled to the Activation of the Co–C Bond in the B12-Dependent Enzyme Ornithine 4,5-Aminomutase: A Computational Study

scientific article published on 19 January 2012

Large-scale Domain Dynamics and Adenosylcobalamin Reorientation Orchestrate Radical Catalysis in Ornithine 4,5-Aminomutase

scientific article

Laser excitation studies of the product release steps in the catalytic cycle of the light-driven enzyme, protochlorophyllide oxidoreductase

scientific article

Laser photoexcitation of NAD(P)H induces reduction of P450 BM3 heme domain on the microsecond time scale

scientific article

Laser-flash photolysis indicates that internal electron transfer is triggered by proton uptake by Alcaligenes xylosoxidans copper-dependent nitrite reductase.

scientific article

Light-driven biocatalytic reduction of α,β-unsaturated compounds by ene reductases employing transition metal complexes as photosensitizers.

scientific article

Light-induced structural changes in a full-length cyanobacterial phytochrome probed by time-resolved X-ray scattering

scientific article published on 03 January 2019

Liver microsomal lipid enhances the activity and redox coupling of colocalized cytochrome P450 reductase-cytochrome P450 3A4 in nanodiscs

scientific article published on 15 June 2017

Lys-D48 is required for charge stabilization, rapid flavin reduction, and internal electron transfer in the catalytic cycle of dihydroorotate dehydrogenase B of Lactococcus lactis

scientific article published on 19 April 2006

Lys300 plays a major role in the catalytic mechanism of maize polyamine oxidase.

scientific article

Machine Learning of Designed Translational Control Allows Predictive Pathway Optimization in Escherichia coli

scientific article published on 07 January 2019

Magnetic Field Effects and Radical Pair Mechanisms in Enzymes: A Reappraisal of the Horseradish Peroxidase System

scientific article published on 01 July 2006

Magnetic Fields Modulate Blue-Light-Dependent Regulation of Neuronal Firing by Cryptochrome

scientific article published on October 2016

Magnetic field effect studies indicate reduced geminate recombination of the radical pair in substrate-bound adenosylcobalamin-dependent ethanolamine ammonia lyase

scientific article published on 28 November 2007

Magnetic field effects as a result of the radical pair mechanism are unlikely in redox enzymes

scientific article published on February 2015

Mass spectrometry locates local and allosteric conformational changes that occur on cofactor binding

scientific article published on 15 July 2016

Mechanism of FAD reduction and role of active site residues His-225 and Tyr-259 in Arthrobacter globiformis dimethylglycine oxidase: analysis of mutant structure and catalytic function

scientific article published on 01 September 2006

Mechanism of coenzyme binding to human methionine synthase reductase revealed through the crystal structure of the FNR-like module and isothermal titration calorimetry

scientific article

Mechanism of radical-based catalysis in the reaction catalyzed by adenosylcobalamin-dependent ornithine 4,5-aminomutase.

scientific article published on 22 October 2008

Mechanistic aspects and redox properties of hyperthermophilic L-proline dehydrogenase from Pyrococcus furiosus related to dimethylglycine dehydrogenase/oxidase

scientific article

Mechanistic aspects of the covalent flavoprotein dimethylglycine oxidase of Arthrobacter globiformis studied by stopped-flow spectrophotometry

scientific article published in April 2002

Mechanistic reappraisal of early stage photochemistry in the light-driven enzyme protochlorophyllide oxidoreductase

scientific article

Modulation of ligand-heme reactivity by binding pocket residues demonstrated in cytochrome c' over the femtosecond-second temporal range.

scientific article published on 11 October 2013

Molecular Determinants of Carbocation Cyclisation in Bacterial Monoterpene Synthases

scientific article published in 2022

Molecular dissection of human methionine synthase reductase: determination of the flavin redox potentials in full-length enzyme and isolated flavin-binding domains

scientific article

Multifragment DNA Assembly of Biochemical Pathways via Automated Ligase Cycling Reaction

scientific article published on 01 January 2018

Multiple active site residues are important for photochemical efficiency in the light-activated enzyme protochlorophyllide oxidoreductase (POR).

scientific article published on June 2016

Mutagenesis alters the catalytic mechanism of the light-driven enzyme protochlorophyllide oxidoreductase

scientific article

Mutagenesis of morphinone reductase induces multiple reactive configurations and identifies potential ambiguity in kinetic analysis of enzyme tunneling mechanisms

scientific article

Nanofibrillar Peptide hydrogels for the immobilization of biocatalysts for chemical transformations

scientific article published on 7 March 2014

Nature of the Energy Landscape for Gated Electron Transfer in a Dynamic Redox Protein

scientific article published on 01 July 2010

New cofactor supports α,β-unsaturated acid decarboxylation via 1,3-dipolar cycloaddition. ⬇️

scientific article published on 17 June 2015

New developments in 'ene'-reductase catalysed biological hydrogenations

scientific article published on 06 March 2014

New insights into the multi-step reaction pathway of the reductive half-reaction catalysed by aromatic amine dehydrogenase: a QM/MM study.

scientific article published in May 2010

New insights into the reductive half-reaction mechanism of aromatic amine dehydrogenase revealed by reaction with carbinolamine substrates

scientific article

Nonequivalence of Second Sphere "Noncatalytic" Residues in Pentaerythritol Tetranitrate Reductase in Relation to Local Dynamics Linked to H-Transfer in Reactions with NADH and NADPH Coenzymes.

scientific article

Nuclear quantum tunneling in the light-activated enzyme protochlorophyllide oxidoreductase

scientific article published on 10 December 2008

Nuclear quantum tunnelling in enzymatic reactions – an enzymologist's perspective

scientific article published on 01 December 2015

O21. Ca2+-calmodulin exerts thermodynamic and conformational regulation over electron transport in nNOS

Observation of the Δg mechanism resulting from the ultrafast spin dynamics that follow the photolysis of coenzyme B12

scientific article published on 01 November 2019

Origin of the Proton-transfer Step in the Cofactor-free (1H)-3-Hydroxy-4-oxoquinaldine 2,4-Dioxygenase: EFFECT OF THE BASICITY OF AN ACTIVE SITE HIS RESIDUE

scientific article

Parallel pathways and free-energy landscapes for enzymatic hydride transfer probed by hydrostatic pressure

scientific article

PartsGenie: an integrated tool for optimising and sharing synthetic biology parts

scholarly article published 15 September 2017

PartsGenie: an integrated tool for optimizing and sharing synthetic biology parts

scholarly article by Neil Swainston published in February 2018

Photochemical Mechanism of Light-Driven Fatty Acid Photodecarboxylase

scientific article published on 19 May 2020

Photochemical Mechanism of an Atypical Algal Phytochrome

scientific article published on 15 March 2018

Photochemical Spin Dynamics of the Vitamin B12 Derivative, Methylcobalamin

scientific article published on 23 May 2019

Photocycle of Cyanobacteriochrome TePixJ

scientific article published on 06 August 2020

Pinpointing a Mechanistic Switch Between Ketoreduction and "Ene" Reduction in Short-Chain Dehydrogenases/Reductases

scientific article published on 13 July 2016

Pinpointing a Mechanistic Switch Between Ketoreduction and "Ene" Reduction in Short-Chain Dehydrogenases/Reductases.

scientific article

Practical aspects on the use of kinetic isotope effects as probes of flavoprotein enzyme mechanisms

scientific article published in January 2014

Pressure Effects on Enzyme-Catalyzed Quantum Tunneling Events Arise from Protein-Specific Structural and Dynamic Changes

scientific article published on 05 June 2012

Probing active site geometry using high pressure and secondary isotope effects in an enzyme-catalysed 'deep' H-tunnelling reaction

scientific article published on July 2010

Probing reversible chemistry in coenzyme B12 -dependent ethanolamine ammonia lyase with kinetic isotope effects

scientific article published on 7 May 2015

Probing the dynamic interface between trimethylamine dehydrogenase (TMADH) and electron transferring flavoprotein (ETF) in the TMADH-2ETF complex: role of the Arg-alpha237 (ETF) and Tyr-442 (TMADH) residue pair

scientific article

Probing the molecular determinants of coenzyme selectivity in the P450 BM3 FAD/NADPH domain

scientific article

Production of Propane and Other Short-Chain Alkanes by Structure-Based Engineering of Ligand Specificity in Aldehyde-Deformylating Oxygenase

scientific article

Production of the Fragrance Geraniol in Peroxisomes of a Product-Tolerant Baker's Yeast

scientific article published on 23 September 2020

Promoter engineering for microbial bio-alkane gas production

scientific article published on 27 October 2020

Promoting motions in enzyme catalysis probed by pressure studies of kinetic isotope effects

scholarly article

Protein Conformational Change Is Essential for Reductive Activation of Lytic Polysaccharide Monooxygenase by Cellobiose Dehydrogenase

scientific article published on 30 March 2020

Protein interactions in the human methionine synthase-methionine synthase reductase complex and implications for the mechanism of enzyme reactivation

scientific article

Protein motions are coupled to the reaction chemistry in coenzyme B12-dependent ethanolamine ammonia lyase

scientific article published on 15 August 2012

Proton transfer in the oxidative half-reaction of pentaerythritol tetranitrate reductase. Structure of the reduced enzyme-progesterone complex and the roles of residues Tyr186, His181, His184.

scientific article

Proton tunneling in aromatic amine dehydrogenase is driven by a short-range sub-picosecond promoting vibration: consistency of simulation and theory with experiment

scientific article published on 17 February 2007

Proton tunnelling and promoting vibrations during the oxidation of ascorbate by ferricyanide?

article

Proton-coupled electron transfer and adduct configuration are important for C4a-hydroperoxyflavin formation and stabilization in a flavoenzyme

scientific article

Proton-coupled electron transfer in the catalytic cycle of Alcaligenes xylosoxidans copper-dependent nitrite reductase

scientific article

Quantum Biology: An Update and Perspective

scientific article published on 26 January 2021

Quantum Mechanics/Molecular Mechanics Studies on the Mechanism of Action of Cofactor Pyridoxal 5′-Phosphate in Ornithine 4,5-Aminomutase

scientific article published on 22 July 2014

Quantum catalysis in enzymes: beyond the transition state theory paradigm. A Discussion Meeting held at the Royal Society on 14 and 15 November 2005. ⬇️

scientific article published in June 2006

Radical-based photoinactivation of fatty acid photodecarboxylases

scientific article published on 26 April 2020

Rapid P450 heme iron reduction by laser photoexcitation of Mycobacterium tuberculosis CYP121 and CYP51B1. Analysis of CO complexation reactions and reversibility of the P450/P420 equilibrium.

scientific article

Rapid prototyping of microbial production strains for the biomanufacture of potential materials monomers

scientific article published on 23 April 2020

Reaction of morphinone reductase with 2-cyclohexen-1-one and 1-nitrocyclohexene: proton donation, ligand binding, and the role of residues Histidine 186 and Asparagine 189.

scientific article published on 4 January 2005

Reaction of vascular adhesion protein-1 (VAP-1) with primary amines: mechanistic insights from isotope effects and quantitative structure-activity relationships

scientific article published on 07 July 2011

Real-time analysis of conformational control in electron transfer reactions of human cytochrome P450 reductase with cytochrome c

scientific article published on 26 August 2015

Reduction of aliphatic nitroesters and N-nitramines by Enterobacter cloacae PB2 pentaerythritol tetranitrate reductase: quantitative structure-activity relationships.

scientific article published on 5 November 2008

Relating localized protein motions to the reaction coordinate in coenzyme B₁₂-dependent enzymes

scientific article published on 21 March 2013

Renewable and tuneable bio-LPG blends derived from amino acids

scientific article published on 14 July 2020

Retooling microorganisms for the fermentative production of alcohols

scientific article published on 5 September 2017

Role of active site residues and solvent in proton transfer and the modulation of flavin reduction potential in bacterial morphinone reductase.

scientific article published on 19 May 2005

Role of histidine 42 in ascorbate peroxidase. Kinetic analysis of the H42A and H42E variants

scientific article published in July 2002

SYNBIOCHEM-a SynBio foundry for the biosynthesis and sustainable production of fine and speciality chemicals

scientific article

Secondary kinetic isotope effects as probes of environmentally-coupled enzymatic hydrogen tunneling reactions

scientific article published in August 2008

SelProm: A Queryable and Predictive Expression Vector Selection Tool for Escherichia coli

scientific article published on 18 March 2019

Selectivity through discriminatory induced fit enables switching of NAD(P)H coenzyme specificity in Old Yellow Enzyme ene-reductases

scientific article published on 13 May 2019

Selenzyme: Enzyme selection tool for pathway design

scientific article published on 7 February 2018

Selenzyme: Enzyme selection tool for pathway design

Solution structure of the two-iron rubredoxin of Pseudomonas oleovorans determined by NMR spectroscopy and solution X-ray scattering and interactions with rubredoxin reductase

scientific article published in March 2004

Solvent as a probe of active site motion and chemistry during the hydrogen tunnelling reaction in morphinone reductase.

scientific article published in September 2008

Solvent-slaved protein motions accompany proton but not hydride tunneling in light-activated protochlorophyllide oxidoreductase

scientific article published in January 2009

Solvent-slaved protein motions accompany proton coupled electron transfer reactions catalysed by copper nitrite reductase

scientific article published on 03 May 2019

Special issue: Catalytic mechanisms by biological systems--introduction

scientific article published on 3 June 2013

Stabilization of non-productive conformations underpins rapid electron transfer to electron-transferring flavoprotein.

scientific article

Stepwise Hydride Transfer in a Biological System: Insights into the Reaction Mechanism of the Light-Dependent Protochlorophyllide Oxidoreductase

scientific article

Stepwise Hydride Transfer in a Biological System: Insights into the Reaction Mechanism of the Light-Dependent Protochlorophyllide Oxidoreductase

Structural Basis of Catalysis in the Bacterial Monoterpene Synthases Linalool Synthase and 1,8-Cineole Synthase.

scientific article published on 9 August 2017

Structural and mechanistic aspects of flavoproteins: probes of hydrogen tunnelling

scientific article published on 03 July 2009

Structural basis for enzymatic photocatalysis in chlorophyll biosynthesis

scientific article published on 23 October 2019

Structural basis of kynurenine 3-monooxygenase inhibition

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Structure and Mechanism of a Viral Collagen Prolyl Hydroxylase

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Structure of the Cannabis sativa olivetol-producing enzyme reveals cyclization plasticity in type III polyketide synthases

scientific article published on 12 October 2019

Structure-Based Insight into the Asymmetric Bioreduction of the C=C Double Bond of alpha,beta-Unsaturated Nitroalkenes by Pentaerythritol Tetranitrate Reductase

scientific article published on November 2008

Structure-based Mechanism of CMP-2-keto-3-deoxymanno-octulonic Acid Synthetase: CONVERGENT EVOLUTION OF A SUGAR-ACTIVATING ENZYME WITH DNA/RNA POLYMERASES

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Structures of carboxylic acid reductase reveal domain dynamics underlying catalysis

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Sweating the assets of flavin cofactors: new insight of chemical versatility from knowledge of structure and mechanism

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Switching pyridine nucleotide specificity in P450 BM3: mechanistic analysis of the W1046H and W1046A enzymes

scientific article published on 14 February 2005

Systematic methodology for the development of biocatalytic hydrogen-borrowing cascades: application to the synthesis of chiral α-substituted carboxylic acids from α-substituted α,β-unsaturated aldehydes

scientific article published in January 2015

Taming the Reactivity of Monoterpene Synthases To Guide Regioselective Product Hydroxylation

scientific article published on 03 December 2019

The catalytic mechanism of aromatic nitration by cytochrome P450 TxtE: Involvement of a ferric-peroxynitrite intermediate

scientific article published on 19 August 2020

The dimeric form of flavocytochrome P450 BM3 is catalytically functional as a fatty acid hydroxylase.

scientific article published on 28 September 2005

The effect of terminal globular domains on the response of recombinant mini-spidroins to fiber spinning triggers

scientific article published on 30 June 2020

The enzyme aromatic amine dehydrogenase induces a substrate conformation crucial for promoting vibration that significantly reduces the effective potential energy barrier to proton transfer.

scientific article published on December 2008

The human apoptosis-inducing protein AMID is an oxidoreductase with a modified flavin cofactor and DNA binding activity

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The pH dependence of kinetic isotope effects in monoamine oxidase A indicates stabilization of the neutral amine in the enzyme-substrate complex.

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The photochemical mechanism of a B12-dependent photoreceptor protein

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The photoinitiated reaction pathway of full-length cyanobacteriochrome Tlr0924 monitored over 12 orders of magnitude

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The sacrificial inactivation of the blue-light photosensor cryptochrome from Drosophila melanogaster

Thermodynamic and kinetic analysis of the isolated FAD domain of rat neuronal nitric oxide synthase altered in the region of the FAD shielding residue Phe1395.

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Thermodynamic basis of electron transfer in dihydroorotate dehydrogenase B from Lactococcus lactis: analysis by potentiometry, EPR spectroscopy, and ENDOR spectroscopy

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Time-resolved studies of radical pairs

scientific article published on April 2009

Towards synthesis of monoterpenes and derivatives using synthetic biology

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Towards the free energy landscape for catalysis in mammalian nitric oxide synthases.

scientific article published on 09 December 2014

Tripping the light fantastic in membrane redox biology: linking dynamic structures to function in ER electron transfer chains.

scientific article published on 30 January 2019

Trp(359) regulates flavin thermodynamics and coenzyme selectivity in Mycobacterium tuberculosis FprA.

scientific article published in May 2008

Tryptophan 697 Modulates Hydride and Interflavin Electron Transfer in Human Methionine Synthase Reductase ⬇️

scientific article published on November 30, 2011

Tryptophan tryptophylquinone cofactor biogenesis in the aromatic amine dehydrogenase of Alcaligenes faecalis. Cofactor assembly and catalytic properties of recombinant enzyme expressed in Paracoccus denitrificans

scientific article published in November 2005

Tunneling and classical paths for proton transfer in an enzyme reaction dominated by tunneling: oxidation of tryptamine by aromatic amine dehydrogenase

scientific article published in March 2007

Two-electron reduction of quinones by Enterobacter cloacae PB2 pentaerythritol tetranitrate reductase: quantitative structure-activity relationships

scientific article published on 04 June 2007

Tyrosyl radical formation and propagation in flavin dependent monoamine oxidases.

scientific article published in June 2010

UbiX is a flavin prenyltransferase required for bacterial ubiquinone biosynthesis

scientific journal article

Ultrafast Vibrational Energy Transfer between Protein and Cofactor in a Flavoenzyme

scientific article published on 04 June 2020

Ultrafast infrared spectral fingerprints of vitamin B12 and related cobalamins

scientific article published in June 2012

Ultrafast red light activation of Synechocystis phytochrome Cph1 triggers major structural change to form the Pfr signalling-competent state

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Unexpected Roles of a Tether Harboring a Tyrosine Gatekeeper Residue in Modular Nitrite Reductase Catalysis

scientific article published on 29 May 2019

Untangling heavy protein and cofactor isotope effects on enzyme- catalyzed hydride transfer.

scientific article published on 27 September 2016

Updated structure of Drosophila cryptochrome

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Using trimethylamine dehydrogenase in an enzyme linked amperometric electrode. Part 1. Wild-type enzyme redox mediation

scientific article published in February 2003

Vertebrate Cryptochromes are Vestigial Flavoproteins

scientific article published on 20 March 2017

What are the signatures of tunnelling in enzyme-catalysed reactions?

scientific article published on 01 December 2019

What's in a covalent bond? On the role and formation of covalently bound flavin cofactors

scientific article published on 05 May 2009

biochem4j: Integrated and extensible biochemical knowledge through graph databases.

scientific article published on 14 July 2017

α-Secondary Isotope Effects as Probes of “Tunneling-Ready” Configurations in Enzymatic H-Tunneling: Insight from Environmentally Coupled Tunneling Models

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