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List of works by Markku Kulomaa

188. Internalization of Novel Delivery Vector TAT-Streptavidin into Human Cells

A Novel Chimeric Avidin with Increased Thermal Stability Using DNA Shuffling

scientific article

A multipurpose vector system for the screening of libraries in bacteria, insect and mammalian cells and expression in vivo

scientific article

Artificial Avidin-Based Receptors for a Panel of Small Molecules

scientific article

Avidin related protein 2 shows unique structural and functional features among the avidin protein family.

scientific article

Baculovirus capsid display: a novel tool for transduction imaging

scientific article published on November 2003

Baculovirus-mediated immediate-early gene expression and nuclear reorganization in human cells

scientific article published on 27 November 2007

Bifunctional avidin with covalently modifiable ligand binding site.

scientific article

Binding Properties of HABA-Type Azo Derivatives to Avidin and Avidin-Related Protein 4

scientific article published on 01 October 2006

Biochemical characterization of CA IX, one of the most active carbonic anhydrase isozymes.

scientific article

Bradavidin II from Bradyrhizobium japonicum: a new avidin-like biotin-binding protein.

scientific article

Brave new (strept)avidins in biotechnology

scientific article published on 12 April 2007

Carbonic anhydrase inhibitors: the very weak inhibitors dithiothreitol, beta-mercaptoethanol, tris(carboxyethyl)phosphine and threitol interfere with the binding of sulfonamides to isozymes II and IX.

scientific article

Characterization of poultry egg-white avidins and their potential as a tool in pretargeting cancer treatment.

scientific article published in May 2003

Chicken avidin-related protein 4/5 shows superior thermal stability when compared with avidin while retaining high affinity to biotin.

scientific article

Chicken avidin-related proteins show altered biotin-binding and physico-chemical properties as compared with avidin.

scientific article published on May 2002

Chicken genome analysis reveals novel genes encoding biotin-binding proteins related to avidin family

scientific article

Chimeric Avidin – NMR Structure and Dynamics of a 56 kDa Homotetrameric Thermostable Protein

scientific article

Chimeric avidin shows stability against harsh chemical conditions--biochemical analysis and 3D structure

scientific article

Cloning and characterization of Scavidin, a fusion protein for the targeted delivery of biotinylated molecules

scientific article published on 12 December 2001

Cloning, expression, post-translational modifications and inhibition studies on the latest mammalian carbonic anhydrase isoform, CA XV.

scientific article published in February 2009

Construction of a dual chain pseudotetrameric chicken avidin by combining two circularly permuted avidins.

scientific article

Construction of chimeric dual-chain avidin by tandem fusion of the related avidins

scientific article

Construction of hevein (Hev b 6.02) with reduced allergenicity for immunotherapy of latex allergy by comutation of six amino acid residues on the conformational IgE epitopes.

scientific article published in February 2004

Controlling quaternary structure assembly: subunit interface engineering and crystal structure of dual chain avidin

scientific article published on 6 May 2006

Covalent Biofunctionalization of Cellulose Acetate with Thermostable Chimeric Avidin

scientific article published on 16 June 2011

Coxsackievirus B3 VLPs purified by ion exchange chromatography elicit strong immune responses in mice.

scientific article published on 28 January 2014

Critical importance of loop conformation to avidin-enhanced hydrolysis of an active biotin ester

scientific article

Crystal structure of rhizavidin: insights into the enigmatic high-affinity interaction of an innate biotin-binding protein dimer

scientific article

DNA family shuffling within the chicken avidin protein family - A shortcut to more powerful protein tools

Design and construction of highly stable, protease-resistant chimeric avidins.

scientific article published on 13 January 2005

Diagnosis of flavobacteriosis by direct amplification of rRNA genes

scientific article published on 01 August 2002

Dimer-Tetramer Transition between Solution and Crystalline States of Streptavidin and Avidin Mutants

scientific article

Dual-affinity avidin molecules.

scientific article published in November 2005

Efficient preparation of shuffled DNA libraries through recombination (Gateway) cloning

scientific article published on December 2014

Efficient production of active chicken avidin using a bacterial signal peptide in Escherichia coli

scientific article published on December 2004

Enhanced gene delivery by avidin-displaying baculovirus

scientific article published on February 2004

Enhancing the thermal stability of avidin. Introduction of disulfide bridges between subunit interfaces

scientific article published on 20 November 2002

Evidence for natural horizontal transfer of the pcpB gene in the evolution of polychlorophenol-degrading sphingomonads.

scientific article published on September 2002

Factors Dictating the Pseudocatalytic Efficiency of Avidins

scientific article published on 03 March 2006

Group-specific quantification of methanotrophs in landfill gas-purged laboratory biofilters by tyramide signal amplification-fluorescence in situ hybridization.

scientific article published on 31 December 2007

High-resolution crystal structure of an avidin-related protein: insight into high-affinity biotin binding and protein stability

scientific article published on 20 April 2005

His-tagged norovirus-like particles: A versatile platform for cellular delivery and surface display.

scientific article published on 10 July 2015

Improvement in nuclear entry and transgene expression of baculoviruses by disintegration of microtubules in human hepatocytes

scientific article

Internalization of novel non-viral vector TAT-streptavidin into human cells

scientific article

Introduction of histidine residues into avidin subunit interfaces allows pH-dependent regulation of quaternary structure and biotin binding

scientific article published on December 2003

Investigating the binding behaviour of two avidin-based testosterone binders using molecular recognition force spectroscopy

scientific article published on 01 February 2014

Isolated hevein-like domains, but not 31-kd endochitinases, are responsible for IgE-mediated in vitro and in vivo reactions in latex-fruit syndrome.

scientific article published in March 2005

Isolation and Characterization of Novosphingobium sp. Strain MT1, a Dominant Polychlorophenol-Degrading Strain in a Groundwater Bioremediation System ⬇️

scientific article published on January 1, 2002

Microbial diversity in a thermophilic aerobic biofilm process: analysis by length heterogeneity PCR (LH-PCR).

scientific article published in May 2003

Modification of the loops in the ligand-binding site turns avidin into a steroid-binding protein

scientific article

Molecular engineering of avidin and hydrophobin for functional self-assembling interfaces

scientific article published on 22 May 2014

Molecular features of steroid-binding antidins and their use for assaying serum progesterone

scientific article published on 20 February 2019

Mutation of the important Tyr-33 residue of chicken avidin: functional and structural consequences

scientific article published on January 2003

Novel avidin-like protein from a root nodule symbiotic bacterium, Bradyrhizobium japonicum.

scientific article published on 28 January 2005

Printable and flexible macroporous organosilica film with high protein adsorption capacity

Production and characterization of virus-like particles and the P domain protein of GII.4 norovirus

scientific article published on 12 May 2011

Production of Hev b5 as a fluorescent biotin-binding tripartite fusion protein in insect cells.

scientific article

Protein and bacterial interactions with nanostructured polymer coatings

scientific article published in October 2015

Purification of norovirus-like particles (VLPs) by ion exchange chromatography

scientific article published on 14 January 2012

Rational Modification of Ligand-Binding Preference of Avidin by Circular Permutation and Mutagenesis

scholarly article by Juha A. E. Määttä published in May 2008

Rational design of an active avidin monomer.

scientific article published on 27 November 2002

Rational modification of ligand-binding preference of avidin by circular permutation and mutagenesis

scientific article

Resonance assignments of the 56 kDa chimeric avidin in the biotin-bound and free forms

scientific article published on 06 March 2012

Rhizavidin from Rhizobium etli: the first natural dimer in the avidin protein family

scientific article

Structural and functional characteristics of xenavidin, the first frog avidin from Xenopus tropicalis

scientific article

Structural characterization of core-bradavidin in complex with biotin

scientific article

Structure and characterization of a novel chicken biotin-binding protein A (BBP-A)

scientific article

Structure of Bradavidin – C-Terminal Residues Act as Intrinsic Ligands

scientific article

Subcellular localization of pentachlorophenol 4-monooxygenase in Sphingobium chlorophenolicum ATCC 39723.

scientific article published in December 2002

Switchavidin: reversible biotin-avidin-biotin bridges with high affinity and specificity.

scientific article published on December 2014

Tetravalent single-chain avidin: from subunits to protein domains via circularly permuted avidins

scientific article published on December 2005

The highly dynamic oligomeric structure of bradavidin II is unique among avidin proteins

scientific article

The major conformational IgE-binding epitopes of hevein (Hev b6.02) are identified by a novel chimera-based allergen epitope mapping strategy.

scientific article

Tuning the “Roadblock” Effect in Kinesin-Based Transport

Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates.

scientific article

Zebavidin - An Avidin-Like Protein from Zebrafish

scientific article

pH-Dependent Deformations of the Energy Landscape of Avidin-like Proteins Investigated by Single Molecule Force Spectroscopy

scientific article published on 18 August 2014

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