List of works by Robert H. Singer

A direct role for FMRP in activity-dependent dendritic mRNA transport links filopodial-spine morphogenesis to fragile X syndrome.

scientific article

A general method to improve fluorophores for live-cell and single-molecule microscopy

scientific article

A new yeast PUF family protein, Puf6p, represses ASH1 mRNA translation and is required for its localization

scientific article

A nucleoporin, Nup60p, affects the nuclear and cytoplasmic localization of ASH1 mRNA in S. cerevisiae.

scientific article

A peptide motif in Raver1 mediates splicing repression by interaction with the PTB RRM2 domain

scientific article

A predominantly nuclear protein affecting cytoplasmic localization of beta-actin mRNA in fibroblasts and neurons

scientific article

A single molecule view of gene expression.

scientific article published on 08 October 2009

A three-camera imaging microscope for high-speed single-molecule tracking and super-resolution imaging in living cells.

scientific article published in August 2015

A transgenic mouse for imaging activity-dependent dynamics of endogenous Arc mRNA in live neurons.

scientific article

A transgenic mouse for in vivo detection of endogenous labeled mRNA.

scientific article

Active transport of the survival motor neuron protein and the role of exon-7 in cytoplasmic localization.

scientific article

Activity-Dependent Trafficking and Dynamic Localization of Zipcode Binding Protein 1 and β-Actin mRNA in Dendrites and Spines of Hippocampal Neurons ⬇️

scientific article published on April 15, 2003

Altered dynamics of intestinal cell maturation in Apc1638N/+ mice.

scientific article

An improved MS2 system for accurate reporting of the mRNA life cycle

scientific article published on 13 November 2017

An unbiased analysis method to quantify mRNA localization reveals its correlation with cell motility.

scientific article

Analyzing mRNA expression using single mRNA resolution fluorescent in situ hybridization

scientific article

Assembling an intermediate filament network by dynamic cotranslation

scientific article

Assembly and Molecular Architecture of the Phosphoinositide 3-Kinase p85α Homodimer

scientific article published on 16 October 2015

Asymmetric distribution of nuclear pore complexes and the cytoplasmic localization of beta2-tubulin mRNA in Chlamydomonas reinhardtii

scientific article published on 01 June 2003

Background free imaging of single mRNAs in live cells using split fluorescent proteins

scientific article published on 09 January 2014

Binding of DEAD-box helicase Dhh1 to the 5'-untranslated region of ASH1 mRNA represses localized translation of ASH1 in yeast cells

scientific article published on 27 April 2017

Blocking beta-catenin binding to the ZBP1 promoter represses ZBP1 expression, leading to increased proliferation and migration of metastatic breast-cancer cells

scientific article published on June 2009

Butyrate and vitamin D3 induce transcriptional attenuation at the cyclin D1 locus in colonic carcinoma cells

scientific article published on March 2009

CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells

scientific article

Calibrating excitation light fluxes for quantitative light microscopy in cell biology.

scientific article

Cell biology of mRNA decay

scientific article

Cellular Levels of Signaling Factors Are Sensed by β-actin Alleles to Modulate Transcriptional Pulse Intensity

scientific article

Cellular Levels of Signaling Factors Are Sensed by β-actin Alleles to Modulate Transcriptional Pulse Intensity

scientific article published on 10 November 2015

Colocalization of different influenza viral RNA segments in the cytoplasm before viral budding as shown by single-molecule sensitivity FISH analysis

scientific article

Correction: Colocalization of Different Influenza Viral RNA Segments in the Cytoplasm before Viral Budding as Shown by Single-molecule Sensitivity FISH Analysis.

scientific article published on 22 July 2013

Cotranscriptional effect of a premature termination codon revealed by live-cell imaging.

scientific article published on 25 October 2011

Direct observation of frequency modulated transcription in single cells using light activation.

scientific article published on 24 September 2013

Dual inhibition of MDMX and MDM2 as a therapeutic strategy in leukemia

scientific article published on 01 April 2018

Dynamic association and localization of human H/ACA RNP proteins

scientific article

Dynamic sorting of nuclear components into distinct nucleolar caps during transcriptional inhibition

scientific article

Dynamic visualization of transcription and RNA subcellular localization in zebrafish

scientific article

Dynamics of single mRNPs in nuclei of living cells.

scientific article

Dynamics of transcription and mRNA export

scientific article

Efficient Bayesian-based multiview deconvolution

scientific article

Feedback regulation between zipcode binding protein 1 and beta-catenin mRNAs in breast cancer cells

scientific article published on 19 May 2008

Fluorescence Imaging Methods to Investigate Translation in Single Cells

scientific article published on 01 April 2019

Fluorescence fluctuation spectroscopy enables quantitative imaging of single mRNAs in living cells

scientific article

Fluorescence in situ hybridization: past, present and future

scientific article

Gene expression and the myth of the average cell

scientific article

Gene expression profiling in single cells within tissue

scientific article

Gene expression within a dynamic nuclear landscape

scientific article

Gene regulation: the HSP70 gene jumps when shocked

scientific article published on May 2014

Glutamate-induced RNA localization and translation in neurons

scientific article published on 17 October 2016

IGF2BP1 promotes cell migration by regulating MK5 and PTEN signaling

scientific article published on January 2012

IMP1 regulates UCA1-mediated cell invasion through facilitating UCA1 decay and decreasing the sponge effect of UCA1 for miR-122-5p.

scientific article published on 18 April 2018

IMP1 suppresses breast tumor growth and metastasis through the regulation of its target mRNAs

scientific article published on 17 February 2016

Identification and testing of a gene expression signature of invasive carcinoma cells within primary mammary tumors

scientific article published in December 2004

Imaging Real-Time Gene Expression in Living Systems with Single-Transcript Resolution: Construct Design and Imaging System Setup

article

Imaging Real-Time Gene Expression in Living Systems with Single-Transcript Resolution: Image Analysis of Single mRNA Transcripts

article

Imaging Real-Time Gene Expression in Living Systems with Single-Transcript Resolution: Single mRNA Particle Tracking with ImageJ-Based Analysis

scientific article

Imaging Real-Time Gene Expression in Living Yeast

article

Imaging Real-Time Gene Expression in Mammalian Cells with Single-Transcript Resolution: Figure 1

article

Imaging Single mRNA Molecules in Mammalian Cells Using an Optimized MS2-MCP System

scientific article published on 01 January 2019

Imaging Transcription: Past, Present, and Future

scientific article

Imaging gene expression in single living cells.

scientific article

Imaging mRNA In Vivo, from Birth to Death

scientific article published on 18 January 2018

Imaging mRNA and protein interactions within neurons

scientific article published on 21 February 2017

Imaging mRNA movement from transcription sites to translation sites

scientific article published on 11 February 2007

Imaging of DNA and RNA in Living Eukaryotic Cells to Reveal Spatiotemporal Dynamics of Gene Expression

scientific article published on 16 March 2020

Imaging of gene expression in living cells and tissues

scientific article

Imaging of single mRNAs in the cytoplasm of living cells

scientific article published on January 2004

Imaging sites of N-wasp activity in lamellipodia and invadopodia of carcinoma cells

scientific article

Imaging transcription in living cells.

scientific article published on January 2009

Imaging translation in single cells using fluorescent microscopy

scientific article

In the right place at the right time: visualizing and understanding mRNA localization.

scientific article

In vivo dynamics of RNA polymerase II transcription.

scientific article published on 05 August 2007

In vivo imaging of labelled endogenous β-actin mRNA during nucleocytoplasmic transport ⬇️

scientific article published on September 15, 2010

Inferring transient particle transport dynamics in live cells

scientific article published on 20 July 2015

Interactions of elongation factor 1alpha with F-actin and beta-actin mRNA: implications for anchoring mRNA in cell protrusions

scientific article

Intercellular mRNA Trafficking Via Membrane Nanotubes In Mammalian Cells

Intercellular mRNA trafficking via membrane nanotube-like extensions in mammalian cells

scientific article published on 24 October 2017

Localization of TFPI-2 in the nucleus modulates MMP-2 gene expression in breast cancer cells

scientific article published on 19 October 2017

Localization of a beta-actin messenger ribonucleoprotein complex with zipcode-binding protein modulates the density of dendritic filopodia and filopodial synapses

scientific journal article

Localization of all seven messenger RNAs for the actin-polymerization nucleator Arp2/3 complex in the protrusions of fibroblasts

scientific article

Macrophage-dependent tumor cell transendothelial migration is mediated by Notch1/MenaINV-initiated invadopodium formation.

scientific article

Mamo decodes hierarchical temporal gradients into terminal neuronal fate

scientific article published on 23 September 2019

Mapping translation 'hot-spots' in live cells by tracking single molecules of mRNA and ribosomes

scientific article published on 13 January 2016

Measurement of barbed ends, actin polymerization, and motility in live carcinoma cells after growth factor stimulation

scientific article published on April 2004

Mechanisms and cellular roles of local protein synthesis in mammalian cells

scientific article published on 02 April 2008

Metabolic cycling in single yeast cells from unsynchronized steady-state populations limited on glucose or phosphate

scientific article

Mitochondrial volume fraction and translation duration impact mitochondrial mRNA localization and protein synthesis

scientific article published on 07 August 2020

Modern fluorescent proteins and imaging technologies to study gene expression, nuclear localization, and dynamics.

scientific article

Multiscale dynamics in nucleocytoplasmic transport.

scientific article published on 22 December 2011

Neurotransmitter identity is acquired in a lineage-restricted manner in the CNS

scholarly article by Haluk Lacin et al published 26 March 2019 in eLife

Novel detection and differential utilization of a c-myc transcriptional block in colon cancer chemoprevention.

scientific article published in November 2002

Nuclear Microenvironments Modulate Transcription From Low-Affinity Enhancers

Nuclear accessibility of β-actin mRNA is measured by 3D single-molecule real-time tracking

scientific article

Nuclear export dynamics of RNA-protein complexes.

scientific article

Nuclear microenvironment in cancer diagnosis and treatment

scientific article published on August 2008

Nuclear microenvironments modulate transcription from low-affinity enhancers.

scientific article published on 2 November 2017

Nuclear pore component Nup98 is a potential tumor suppressor and regulates posttranscriptional expression of select p53 target genes

scientific article published on 24 October 2012

Photoswitchable red fluorescent protein with a large Stokes shift

scientific article

Promoter-Autonomous Functioning in a Controlled Environment using Single Molecule FISH.

scientific article published on 28 May 2015

Promotion of importin alpha-mediated nuclear import by the phosphorylation-dependent binding of cargo protein to 14-3-3

scientific article

Protocol for using TRIBE to study RNA-protein interactions and nuclear organization in mammalian cells

scientific article published on 30 June 2021

QNQKE targeting motif for the SMN-Gemin multiprotein complexin neurons

scientific article

Quantifying Protein-mRNA Interactions in Single Live Cells

scientific article published on July 2015

Quantitative mRNA imaging throughout the entire Drosophila brain

scientific article

RNA asymmetric distribution and daughter/mother differentiation in yeast

scientific article published on December 2003

RNA localization

scientific article published on September 2005

RNA processing and export

scientific article

RNP transport in cell biology: the long and winding road

scientific article published on 28 February 2017

Re-targeting of macroH2A following mitosis to cytogenetic-scale heterochromatic domains

Real-time observation of transcription initiation and elongation on an endogenous yeast gene

scientific article

Regulation of local expression of cell adhesion and motility-related mRNAs in breast cancer cells by IMP1/ZBP1

scientific article

Retargeting of macroH2A following mitosis to cytogenetic-scale heterochromatic domains

scientific article published on 20 May 2019

Role of the parafusin orthologue, PRP1, in microneme exocytosis and cell invasion in Toxoplasma gondii.

scientific article published in September 2003

Rrp17p is a eukaryotic exonuclease required for 5' end processing of Pre-60S ribosomal RNA

scientific article

Sam68 Enables Metabotropic Glutamate Receptor-Dependent LTD in Distal Dendritic Regions of CA1 Hippocampal Neurons

scientific article published on 01 November 2019

She2p Is a Novel RNA Binding Protein with a Basic Helical Hairpin Motif

article

Single cell analysis of RNA-mediated histone H3.3 recruitment to a cytomegalovirus promoter-regulated transcription site.

scientific article

Single cell behavior in metastatic primary mammary tumors correlated with gene expression patterns revealed by molecular profiling

article

Single mRNA molecules demonstrate probabilistic movement in living mammalian cells

scientific article

Single mRNA tracking in live cells

scientific article

Single molecule mRNA fluorescent in situ hybridization combined with immunofluorescence in S. cerevisiae: Dataset and quantification

scientific article published on 11 April 2020

Single β-actin mRNA detection in neurons reveals a mechanism for regulating its translatability.

scientific article

Single-Cell and Single-Molecule Analysis of Gene Expression Regulation.

scientific article

Single-RNA counting reveals alternative modes of gene expression in yeast

scientific article

Single-cell gene expression profiling

scientific article

Single-cell transcription site activation predicts chemotherapy response in human colorectal tumors

scientific article

Single-mRNA counting using fluorescent in situ hybridization in budding yeast

scientific article

Single-mRNA detection in living S. cerevisiae using a re-engineered MS2 system

scientific article published on 01 October 2018

Single-molecule analysis of gene expression using two-color RNA labeling in live yeast

scientific article

Single-molecule imaging of transcription dynamics in somatic stem cells

scientific article published on 24 June 2020

Single-molecule insights into mRNA dynamics in neurons

scientific article published on 4 June 2015

Single-molecule mRNA decay measurements reveal promoter- regulated mRNA stability in yeast

scientific article

Spatial arrangement of an RNA zipcode identifies mRNAs under post-transcriptional control

scientific article published on January 2012

Spatial regulation of beta-actin translation by Src-dependent phosphorylation of ZBP1.

scientific article published on November 2005

Specific interaction of KIF11 with ZBP1 regulates the transport of β-actin mRNA and cell motility

scientific article

Stepwise RNP assembly at the site of H/ACA RNA transcription in human cells

scientific article

Subnuclear positioning and interchromosomal clustering of the GAL1-10 locus are controlled by separable, interdependent mechanisms

scientific article published on 03 August 2016

Synonymous modification results in high-fidelity gene expression of repetitive protein and nucleotide sequences.

scientific article

Temporal and spatial characterization of nonsense-mediated mRNA decay

scientific article published on 21 February 2013

The PTB interacting protein raver1 regulates alpha-tropomyosin alternative splicing

scientific article published on December 2003

The cytoplasmic fate of an mRNP is determined cotranscriptionally: exception or rule?

scientific article published on September 2010

The dynamic range of transcription

scientific article published on June 2008

The dynamics of mammalian P body transport, assembly, and disassembly in vivo

scientific article

The fate of the messenger is pre-determined: a new model for regulation of gene expression

scientific article published on 19 January 2013

The life of an mRNA in space and time

scientific article

The spatial order of transcription in mammalian cells.

scientific article published on October 2007

The structural basis for RNA selectivity by the IMP family of RNA-binding proteins

scientific article published on 30 September 2019

The translation elongation factor eEF1A1 couples transcription to translation during heat shock response

scientific article published in 2014

The travels of mRNAs in neurons: do they know where they are going?

scientific article published on 19 February 2019

Time-integrated fluorescence cumulant analysis and its application in living cells

scientific article

Transcription of functionally related constitutive genes is not coordinated.

scientific article

Transcriptional pulsing of a developmental gene

scientific article

Translation dynamics of single mRNAs in live cells and neurons

scientific article

Translation of ASH1 mRNA is repressed by Puf6p-Fun12p/eIF5B interaction and released by CK2 phosphorylation

scientific article published on April 2008

Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals.

scientific article

Transvection Goes Live-Visualizing Enhancer-Promoter Communication between Chromosomes.

scientific article published in April 2018

Visualization of dynamics of single endogenous mRNA labeled in live mouse

scientific article

Visualization of mRNA translation in living cells

scientific article

YRA1 autoregulation requires nuclear export and cytoplasmic Edc3p-mediated degradation of its pre-mRNA.

scientific article published on February 2007

ZBP1 enhances cell polarity and reduces chemotaxis

scientific article published on September 2007

ZBP1 regulates mRNA stability during cellular stress

scientific article

ZBP2 Facilitates Binding of ZBP1 to β-Actin mRNA during Transcription.

scientific article published in May 2009

ZBP2 facilitates binding of ZBP1 to beta-actin mRNA during transcription

scientific article published on 24 September 2007

Zipcode Binding Protein 1 (ZBP1; IGF2BP1): A Model for Sequence-Specific RNA Regulation

scientific article published on 21 February 2020

mRNA on the move: the road to its biological destiny

scientific article

β-Actin mRNA compartmentalization enhances focal adhesion stability and directs cell migration

scientific article published on September 2012

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