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List of works by Andreas Plückthun

A Biotin Ligase-Based Assay for the Quantification of the Cytosolic Delivery of Therapeutic Proteins

scientific article published on January 2017

A Library-Based Screening Strategy for the Identification of DARPins as Ligands for Receptor-Targeted AAV and Lentiviral Vectors

article

A cleavable ligand column for the rapid isolation of large quantities of homogeneous and functional neurotensin receptor 1 variants from E. coli

scientific article

A combined NMR and computational approach to investigate peptide binding to a designed Armadillo repeat protein

scientific article published on 24 March 2015

A comparison of strategies to stabilize immunoglobulin Fv-fragments

scientific article published in February 1990

A designed ankyrin repeat protein evolved to picomolar affinity to Her2

scientific article

A designed ankyrin repeat protein selected to bind to tubulin caps the microtubule plus end

scientific article

A dimeric bispecific miniantibody combines two specificities with avidity

scientific article published on 01 July 1998

A generic selection system for improved expression and thermostability of G protein-coupled receptors by directed evolution

scientific article published on 18 February 2016

A heterodimeric coiled-coil peptide pair selected in vivo from a designed library-versus-library ensemble.

scientific article

A label-free immunosensor array using single-chain antibody fragments

scientific article

A mutation designed to alter crystal packing permits structural analysis of a tight-binding fluorescein-scFv complex

scientific article

A natural antibody missing a cysteine in VH: consequences for thermodynamic stability and folding.

scientific article

A novel fusion toxin derived from an EpCAM-specific designed ankyrin repeat protein has potent antitumor activity

scientific article published on 12 November 2010

A novel strategy to design binding molecules harnessing the modular nature of repeat proteins.

scientific article published in March 2003

A quantitative comparison of cytosolic delivery via different protein uptake systems.

scientific article published on 16 October 2017

A survival selection strategy for engineering synthetic binding proteins that specifically recognize post-translationally phosphorylated proteins

scientific article published on 23 April 2019

A universal approach to prepare reagents for DNA-assisted protein analysis.

scientific article

A versatile and highly repressible Escherichia coli expression system based on invertible promoters: expression of a gene encoding a toxic product.

scientific article

Adenoviral vector with shield and adapter increases tumor specificity and escapes liver and immune control.

scientific article published on 31 January 2018

Advanced analyses of kinetic stabilities of iggs modified by mutations and glycosylation

scientific article published on 11 June 2015

Advances in the design and engineering of peptide-binding repeat proteins

scientific article published on 16 September 2016

Affinity-matured recombinant antibody fragments analyzed by single-molecule force spectroscopy

scientific article

Allosteric inhibition of aminoglycoside phosphotransferase by a designed ankyrin repeat protein

scientific article published in August 2005

Amyloid-β peptide-specific DARPins as a novel class of potential therapeutics for Alzheimer disease

scientific article

An Interface-Driven Design Strategy Yields a Novel, Corrugated Protein Architecture

article by Mohammad ElGamacy et al published 27 August 2018 in ACS Synthetic Biology

An antibody library for stabilizing and crystallizing membrane proteins - selecting binders to the citrate carrier CitS.

scientific article published in April 2004

An automated iterative approach for protein structure refinement using pseudocontact shifts

scientific article published on 02 August 2021

An intrinsically stable antibody scFv fragment can tolerate the loss of both disulfide bonds and fold correctly

scientific article published in May 1998

Analysis of IgG kinetic stability by differential scanning calorimetry, probe fluorescence and light scattering.

scientific article

Animal-free alternatives and the antibody iceberg

scientific article published on 12 October 2020

Antibodies from Escherichia coli

scientific article

Antibody engineering: advances from the use of Escherichia coli expression systems

scientific article published on June 1, 1991

Antibody scFv fragments without disulfide bonds made by molecular evolution

Antibody–Drug Conjugates for Tumor Targeting—Novel Conjugation Chemistries and the Promise of non-IgG Binding Proteins

Antigen binding forces of individually addressed single-chain Fv antibody molecules.

scientific article

Antitumor activity of an epithelial cell adhesion molecule targeted nanovesicular drug delivery system

scientific article published on November 2007

Assembly of a functional immunoglobulin Fv fragment in Escherichia coli

scientific article published on May 1988

Assessment of ab initio models of protein complexes by molecular dynamics. ⬇️

scientific article

Assignments of 1H and 15N resonances of the bacteriophage lambda capsid stabilizing protein gpD.

scientific article

Asymmetric requirement of Dpp/BMP morphogen dispersal in the Drosophila wing disc

scientific article published in 2021

Atomic Force Microscopy Detects Changes in the Interaction Forces between GroEL and Substrate Proteins ⬇️

scientific article published on June 1, 1998

Author Correction: Asymmetric requirement of Dpp/BMP morphogen dispersal in the Drosophila wing disc

correction of a scholarly article

BIACORE analysis of histidine-tagged proteins using a chelating NTA sensor chip

scientific article published in October 1997

Biophysical Properties of Camelid VHHDomains Compared to Those of Human VH3 Domains†

scientific article published on 01 March 2002

Biophysical properties of human antibody variable domains

scientific article

Biotechnological aspects of antibody production in E. coli

article

Bispecific designed ankyrin repeat proteins (DARPins) targeting epidermal growth factor receptor inhibit A431 cell proliferation and receptor recycling

scientific article published on 6 October 2011

Changes to International Nonproprietary Names for antibody therapeutics 2017 and beyond: of mice, men and more

scientific article published on 16 June 2017

Chaperone-assisted structure elucidation with DARPins

scientific article published on 06 January 2020

Characterization and further stabilization of designed ankyrin repeat proteins by combining molecular dynamics simulations and experiments

scientific article published on 21 September 2007

Characterization of the linker peptide of the single-chain Fv fragment of an antibody by NMR spectroscopy

scientific article published on 01 April 1993

Characterizing the functionality of recombinant T-cell receptors in vitro: a pMHC tetramer based approach

scientific article published on 01 March 2000

Chemosensitization of carcinoma cells using epithelial cell adhesion molecule-targeted liposomal antisense against bcl-2/bcl-xL.

scientific article published on December 2006

Circular β-lactamase: stability enhancement by cyclizing the backbone

scientific article published on 01 October 1999

Co-crystallization with conformation-specific designed ankyrin repeat proteins explains the conformational flexibility of BCL-W

scientific article

Co-selection of cognate antibody-antigen pairs by selectively-infective phages

scientific article

Combinatorial approaches to novel proteins

scientific article published in February 2004

Comparison of in vivo selection and rational design of heterodimeric coiled coils

scientific article

Comparison of the amide proton exchange behavior of the rapidly formed folding intermediate and the native state of an antibody scFv fragment

scientific article published on 01 April 1997

Competition BIAcore for measuring true affinities: large differences from values determined from binding kinetics

scientific article published in February 1996

Comprehensive analysis of heterotrimeric G-protein complex diversity and their interactions with GPCRs in solution

scientific article

Computational Modeling of Designed Ankyrin Repeat Protein Complexes with Their Targets

scientific article published on 11 May 2019

Computational analysis of off-rate selection experiments to optimize affinity maturation by directed evolution

scientific article

Computationally Designed Armadillo Repeat Proteins for Modular Peptide Recognition

scientific article

Conformation of fatty acyl chains in α- and β-phosphatidylcholine and phosphatidylethanolamine derivatives in sonicated vesicles

scientific article published on 01 March 1986

Conformation-dependent recognition of HIV gp120 by designed ankyrin repeat proteins provides access to novel HIV entry inhibitors

scientific article published on 13 March 2013

Conformational changes and spatial arrangement of the E. coli chaperones GroEL and GroES

scholarly article by R. Stegmann et al published June 1997 in Physica B

Conformational dynamics of a G-protein α subunit is tightly regulated by nucleotide binding

scientific article published on 13 June 2016

Consensus design of repeat proteins.

scientific article published in February 2004

Construction and characterization of a kappa opioid receptor devoid of all free cysteines

scientific article published on January 2004

Construction and characterization of protein libraries composed of secondary structure modules

scientific article published in November 2002

Correctly Folded T-cell Receptor Fragments in the Periplasm of Escherichia coli

scholarly article by Christoph Wülfing & Andreas Plückthun published October 1994 in Journal of Molecular Biology

Correlation between in Vitro Stability and in Vivo Performance of Anti-GCN4 Intrabodies as Cytoplasmic Inhibitors

scientific article published in Journal of Biological Chemistry

Covalently circularized nanodiscs for studying membrane proteins and viral entry

scientific article

Critical features for biosynthesis, stability, and functionality of a G protein-coupled receptor uncovered by all-versus-all mutations

scientific article

Crystal Structure and Function of a DARPin Neutralizing Inhibitor of Lactococcal Phage TP901-1: COMPARISON OF DARPin AND CAMELID VHH BINDING MODE

scientific article

Crystal structure of a consensus-designed ankyrin repeat protein: Implications for stability

scientific article published on 01 November 2006

Crystal structure of a truncated version of the phage lambda protein gpD.

scientific article published in December 2004

Crystal structure of the anti-His tag antibody 3D5 single-chain fragment complexed to its antigen

scientific article

Crystal structure of the dimeric C-terminal domain of TonB reveals a novel fold

scientific article

Crystal structure of the human oxytocin receptor

scientific article published on 15 July 2020

Crystal structure of the α<sub>1B</sub>-adrenergic receptor reveals molecular determinants of selective ligand recognition

scientific article published on 19 January 2022

Crystal structures of designed armadillo repeat proteins: implications of construct design and crystallization conditions on overall structure.

scientific article

Crystal structures of the human neurokinin 1 receptor in complex with clinically used antagonists

scientific article published in Nature Communications

Crystallization and preliminary X-ray studies of the VL domain of the antibody McPC603 produced in Escherichia coli.

scientific article

Curvature of designed armadillo repeat proteins allows modular peptide binding

scientific article published on 29 August 2017

Cyclic green fluorescent protein produced in vivo using an artificially split PI-PfuI intein from Pyrococcus furiosus.

scientific article

DARPin-Based Crystallization Chaperones Exploit Molecular Geometry as a Screening Dimension in Protein Crystallography

scientific article

DARPin-targeting of measles virus: unique bispecificity, effective oncolysis, and enhanced safety

scientific article published on 5 February 2013

DARPins and other repeat protein scaffolds: advances in engineering and applications

scientific article

DARPins recognizing mTFP1 as novel reagents for and protein manipulations

scientific article published on 29 October 2018

DARPins recognizing the tumor-associated antigen EpCAM selected by phage and ribosome display and engineered for multivalency

scientific article

DARPins: an efficient targeting domain for lentiviral vectors

scientific article

Design and Characterization of Modular Scaffolds for Tubulin Assembly

scientific article

Design and applications of a clamp for Green Fluorescent Protein with picomolar affinity.

scientific article published on 24 November 2017

Design of multivalent complexes using the barnase*barstar module

scientific article published on 23 November 2003

Designed Armadillo repeat proteins: library generation, characterization and selection of peptide binders with high specificity.

scientific article

Designed ankyrin repeat proteins (DARPins) as novel isoform-specific intracellular inhibitors of c-Jun N-terminal kinases

scientific article

Designed ankyrin repeat proteins (DARPins) from research to therapy

scientific article

Designed ankyrin repeat proteins (DARPins): binding proteins for research, diagnostics, and therapy

scientific article

Designed ankyrin repeat proteins: a novel tool for testing epidermal growth factor receptor 2 expression in breast cancer.

scientific article published on 21 May 2010

Designed armadillo repeat proteins as general peptide-binding scaffolds: consensus design and computational optimization of the hydrophobic core

scientific article

Designed to be stable: crystal structure of a consensus ankyrin repeat protein

scientific article

Designing repeat proteins: modular leucine-rich repeat protein libraries based on the mammalian ribonuclease inhibitor family.

scientific article

Designing repeat proteins: well-expressed, soluble and stable proteins from combinatorial libraries of consensus ankyrin repeat proteins.

scientific article

Destabilization of the complete protein secondary structure on binding to the chaperone GroEL.

scientific article published in March 1994

Destabilizing an interacting motif strengthens the association of a designed ankyrin repeat protein with tubulin

scientific article published on 06 July 2016

Development of a generic adenovirus delivery system based on structure-guided design of bispecific trimeric DARPin adapters

scientific article

Development of the designed ankyrin repeat protein (DARPin) G3 for HER2 molecular imaging

scientific article

Different Equilibrium Stability Behavior of ScFv Fragments: Identification, Classification, and Improvement by Protein Engineering†

scientific article published in 1999

Direct Molecular Evolution of Detergent-Stable G Protein-Coupled Receptors Using Polymer Encapsulated Cells ⬇️

scientific article published on November 16, 2012

Direct Selection of Antibodies from Complex Libraries with the Protein Fragment Complementation Assay

Direct identification of ligand-receptor interactions on living cells and tissues

scientific article

Direct in vivo screening of intrabody libraries constructed on a highly stable single-chain framework

scientific article

Directed evolution of G-protein-coupled receptors for high functional expression and detergent stability

scientific article published in January 2013

Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity

scientific article

Directed evolution of an anti-prion protein scFv fragment to an affinity of 1 pM and its structural interpretation

scientific article published on 21 July 2006

Directed in vitro evolution and crystallographic analysis of a peptide-binding single chain antibody fragment (scFv) with low picomolar affinity

scientific article

Disrupting the hydrophobic patches at the antibody variable/constant domain interface: improved in vivo folding and physical characterization of an engineered scFv fragment

scientific article published in April 1997

Distinct conformations of the HIV-1 V3 loop crown are targetable for broad neutralization

scientific article published on 18 November 2021

Domain interactions in antibody Fv and scFv fragments: effects on unfolding kinetics and equilibria

scientific article published on 01 December 1999

Domain interactions in the Fab fragment: a comparative evaluation of the single-chain Fv and Fab format engineered with variable domains of different stability

scientific article

Effect of single point mutations in citrate synthase on binding to GroEL

scientific article published on 01 February 1996

Effects of overexpressing folding modulators on the in vivo folding of heterologous proteins in Escherichia coli

scientific article

Efficient cell-specific uptake of binding proteins into the cytoplasm through engineered modular transport systems

scientific article published on 17 December 2014

Efficient selection of DARPins with sub-nanomolar affinities using SRP phage display

scientific article

Efficient tumor targeting with high-affinity designed ankyrin repeat proteins: effects of affinity and molecular size

scientific article

Electron Microscopy of the GroEL-GroES Filament

Engineered proteins as specific binding reagents.

scientific article published in August 2005

Engineered proteins with desired specificity: DARPins, other alternative scaffolds and bispecific IgGs.

scientific article

Engineering Af1521 improves ADP-ribose binding and identification of ADP-ribosylated proteins

scientific article published on 15 October 2020

Engineering Aggregation Resistance in IgG by Two Independent Mechanisms: Lessons from Comparison of Pichia pastoris and Mammalian Cell Expression

Engineering an anti-HER2 biparatopic antibody with a multimodal mechanism of action

scientific article published on 18 June 2021

Engineering and functional immobilization of opioid receptors

scientific article

Engineering novel binding proteins from nonimmunoglobulin domains

scientific article published in October 2005

Enhanced lysis by bispecific oncolytic measles viruses simultaneously using HER2/neu or EpCAM as target receptors

scientific article published on 24 February 2016

EpCAM-targeted delivery of nanocomplexed siRNA to tumor cells with designed ankyrin repeat proteins

scientific article

Epithelial cell adhesion molecule-targeted drug delivery for cancer therapy.

scientific article published on 14 January 2013

Erratum to “Designed Armadillo Repeat Proteins: Library Generation, Characterization and Selection of Peptide Binders with High Specificity” [J. Mol. Biol. (2012) 424, 68–87]

scholarly article published in Journal of Molecular Biology

Evolution of three human GPCRs for higher expression and stability

scientific article published on 3 March 2011

Facile Site-Specific Multiconjugation Strategies in Recombinant Proteins Produced in Bacteria

scientific article published on 01 January 2019

Facile double-functionalization of designed ankyrin repeat proteins using click and thiol chemistries

scientific article published on 20 January 2012

Facile promoter deletion in Escherichia coli in response to leaky expression of very robust and benign proteins from common expression vectors

scientific article published on 26 January 2009

Factors Influencing the Dimer to Monomer Transition of an Antibody Single-Chain Fv Fragment†

article by Katja M. Arndt et al published September 1998 in Biochemistry

Fast selection of antibodies without antigen purification: adaptation of the protein fragment complementation assay to select antigen-antibody pairs.

scientific article

Filamentous phage infection: crystal structure of g3p in complex with its coreceptor, the C-terminal domain of TolA

scientific article

Flavonol-mediated stabilization of PIN efflux complexes regulates polar auxin transport

scientific article published on 13 November 2020

Folding Nuclei of the scFv Fragment of an Antibody†

scientific article published in 1996

Folding and assembly of an antibody Fv fragment, a heterodimer stabilized by antigen 1 1Edited by P. E. Wright

Folding and unfolding mechanism of highly stable full-consensus ankyrin repeat proteins.

scientific article

Folding in vitro and transport in vivo of pre-β-lactamase are SecB independent

scientific article

Folding intermediates of β-lactamase recognized by GroEL

scientific article published on 01 January 1997

Folding of a designed simple ankyrin repeat protein.

scientific article

Forces and energetics of hapten-antibody dissociation: a biased molecular dynamics simulation study

scientific article published in November 2001

From DARPins to LoopDARPins: novel LoopDARPin design allows the selection of low picomolar binders in a single round of ribosome display

scientific article

Functional and dynamic polymerization of the ALS-linked protein TDP-43 antagonizes its pathologic aggregation

scientific article

Functional antibody single-chain fragments from the cytoplasm of Escherichia coli: influence of thioredoxin reductase (TrxB).

scientific article

G-quadruplexes are specifically recognized and distinguished by selected designed ankyrin repeat proteins

scientific article

Generation of Fluorogen-Activating Designed Ankyrin Repeat Proteins (FADAs) as Versatile Sensor Tools

scientific article published on 23 January 2016

Getting to reproducible antibodies: the rationale for sequenced recombinant characterized reagents

scientific article published in October 2015

GroE prevents the accumulation of early folding intermediates of pre-beta-lactamase without changing the folding pathway.

scientific article published in March 1992

GroEL walks the fine line: the subtle balance of substrate and co-chaperonin binding by GroEL. A combinatorial investigation by design, selection and screening

scientific article published on 20 December 2005

Helix-stabilized fv (hsfv) antibody fragments: substituting the constant domains of a fab fragment for a heterodimeric coiled-coil domain 1 1Edited by I. A. Wilson

article published in 2001

Hemolytic assay for venom phospholipase A2.

scientific article published in December 1981

Her2-specific multivalent adapters confer designed tropism to adenovirus for gene targeting

scientific article

High enzymatic activity and chaperone function are mechanistically related features of the dimeric E. coli peptidyl-prolyl-isomerase FkpA.

scientific article

High-Throughput Fluorescence Polarization Assay to Identify Ligands Using Purified G Protein-Coupled Receptor

scientific article published on 29 March 2019

High-Throughput Generation of Bispecific Binding Proteins by Sortase A-Mediated Coupling for Direct Functional Screening in Cell Culture

scientific article published on 23 December 2019

High-Throughput Quantification of Surface Protein Internalization and Degradation

scientific article published on 14 May 2019

High-affinity binders selected from designed ankyrin repeat protein libraries

scientific article

High-resolution crystal structure of parathyroid hormone 1 receptor in complex with a peptide agonist

scientific article published on 19 November 2018

Identification of a functional epitope of the Nogo receptor by a combinatorial approach using ribosome display

scientific article published in September 2005

Identification of the Binding Surface on β-Lactamase for GroEL by Limited Proteolysis and MALDI-Mass Spectrometry ⬇️

scientific article published on August 18, 1998

Imaging the native structure of the chaperone protein GroEL without fixation using atomic force microscopy

scientific article published on 01 August 2001

Improving Expression of scFv Fragments by Co-expression of Periplasmic Chaperones

Improving in vivo folding and stability of a single-chain Fv antibody fragment by loop grafting

scientific article

Improving the apo-state detergent stability of NTS1 with CHESS for pharmacological and structural studies

scientific article published on 23 July 2014

In vitro display technologies: novel developments and applications

scientific article (publication date: August 2001)

In vitro generated antibodies specific for telomeric guanine-quadruplex DNA react with Stylonychia lemnae macronuclei

scientific article (publication date: 17 July 2001)

In vitro selection and characterization of DARPins and Fab fragments for the co-crystallization of membrane proteins: The Na(+)-citrate symporter CitS as an example

article published in 2007

In vitro selection and evolution of functional proteins by using ribosome display

scientific article

In vitro selection for catalytic activity with ribosome display

scientific article

In vivo assembly and large-scale purification of a GPCR - Gα fusion with Gβγ, and characterization of the active complex

scientific article published in PLoS ONE

In-vitro protein evolution by ribosome display and mRNA display

scientific article

Increasing the antitumor effect of an EpCAM-targeting fusion toxin by facile click PEGylation

scientific article published on November 2013

Individual filamentous phage imaged by electron holography

scientific article

Influence of size and charge of unstructured polypeptides on pharmacokinetics and biodistribution of targeted fusion proteins

scientific article published on 25 June 2019

Inhibition of caspase-2 by a designed ankyrin repeat protein: specificity, structure, and inhibition mechanism

scientific article

Inhibition of the MET Kinase Activity and Cell Growth in MET-Addicted Cancer Cells by Bi-Paratopic Linking

scientific article published on 28 March 2019

Insight into microtubule nucleation from tubulin-capping proteins

scientific article published on 29 April 2019

Insight into odorant perception: the crystal structure and binding characteristics of antibody fragments directed against the musk odorant traseolide

scientific article

Intermolecular biparatopic trapping of ErbB2 prevents compensatory activation of PI3K/AKT via RAS-p110 crosstalk

scientific article

Intracellular kinase inhibitors selected from combinatorial libraries of designed ankyrin repeat proteins

scientific article

Isolation of intracellular proteinase inhibitors derived from designed ankyrin repeat proteins by genetic screening

scientific article published on 18 October 2006

Kinetic stability and crystal structure of the viral capsid protein SHP.

scientific article published in November 2004

Knowledge-based design of a biosensor to quantify localized ERK activation in living cells

scientific article

Knowledge-based design of reagentless fluorescent biosensors from a designed ankyrin repeat protein

scientific article

Labeling surface proteins with high specificity: Intrinsic limitations of phosphopantetheinyl transferase systems

scientific article published on 19 December 2019

Ligand Discovery for a Peptide-Binding GPCR by Structure-Based Screening of Fragment- and Lead-Like Chemical Libraries

scientific article

Ligand binding of a ribosome-displayed protein detected in solution at the single molecule level by fluorescence correlation spectroscopy

Liposome functionalization with copper-free "click chemistry".

scientific article published on 24 January 2015

Lung macrophage scavenger receptor SR-A6 (MARCO) is an adenovirus type-specific virus entry receptor.

scientific article

Malignant tissues produce divergent antibody glycosylation of relevance for cancer gene therapy effectiveness

scientific article published on 09 July 2020

Mapping and modification of an antibody hapten binding site: a site-directed mutagenesis study of McPC603.

scientific article published in March 1991

Maximizing detergent stability and functional expression of a GPCR by exhaustive recombination and evolution

scientific article

Miniantibodies: use of amphipathic helices to produce functional, flexibly linked dimeric FV fragments with high avidity in Escherichia coli

scientific article published on February 1992

Model and Simulation of Multivalent Binding to Fixed Ligands

article

Modification of the kinetic stability of immunoglobulin G by solvent additives

Modular peptide binding: from a comparison of natural binders to designed armadillo repeat proteins.

scientific article

Molecular dynamics study of the stabilities of consensus designed ankyrin repeat proteins

article

Monovalent antibody scFv fragments selected to modulate T-cell activation by inhibition of CD86-CD28 interaction

scientific journal article

Mono‐ and Bivalent Antibody Fragments Produced in Escherichia coli: Engineering, Folding and Antigen Binding ⬇️

scientific article published on December 1, 1992

Multiple cycles of global unfolding of GroEL-bound cyclophilin A evidenced by NMR ⬇️

scientific article published on September 5, 1997

Multispecific Targeting with Synthetic Ankyrin Repeat Motif Chimeric Antigen Receptors

scientific article published on 23 September 2019

Mutations in sigma 70 transcription factor improves expression of functional eukaryotic membrane proteins in Escherichia coli

scientific article published on 21 February 2019

Mutual Stabilization of VLand VHin Single-Chain Antibody Fragments, Investigated with Mutants Engineered for Stability†

article by Arne Wörn & Andreas Plückthun published September 1998 in Biochemistry

NK cells with tissue-resident traits shape response to immunotherapy by inducing adaptive antitumor immunity

scientific article published in 2022

NMR solution structure of the monomeric form of the bacteriophage λ capsid stabilizing protein gpD

scientific article published on 01 April 2005

New protein engineering approaches to multivalent and bispecific antibody fragments

scientific article published on June 1997

New views into class B GPCRs from the crystal structure of PTH1R

scientific article published on 31 October 2019

Non-repetitive single-chain Fv linkers selected by selectively infective phage (SIP) technology ⬇️

scientific article published on May 1, 1998

Novel fold and capsid-binding properties of the lambda-phage display platform protein gpD

scientific article

Novel prodrug-like fusion toxin with protease-sensitive bioorthogonal PEGylation for tumor targeting

scientific article

Off-target-free gene delivery by affinity-purified receptor-targeted viral vectors

scientific article published on 10 February 2015

Optimization of designed armadillo repeat proteins by molecular dynamics simulations and NMR spectroscopy

scientific article

Orthogonal Assembly of a Designed Ankyrin Repeat Protein–Cytotoxin Conjugate with a Clickable Serum Albumin Module for Half-Life Extension

PEGylation and multimerization of the anti-p185HER-2 single chain Fv fragment 4D5: effects on tumor targeting

scientific article published on 8 September 2006

PIN-bodies: a new class of antibody-like proteins with CD4 specificity derived from the protein inhibitor of neuronal nitric oxide synthase.

scientific article

Parallel pathways in the folding of a short-term denatured scFv fragment of an antibody

scientific article published on 01 January 1998

Peptide binding affinity redistributes preassembled repeat protein fragments

scientific article published on 01 February 2019

Peptide-Guided Assembly of Repeat Protein Fragments

scientific article published on 26 February 2018

Personalised proteome analysis by means of protein microarrays made from individual patient samples

scientific article

Phase Behavior of a Designed Cyclopropyl Analogue of Monoolein: Implications for Low-Temperature Membrane Protein Crystallization

scientific article published on 21 November 2014

Production of recombinant human beta2-microglobulin for scintigraphic diagnosis of amyloidosis in uremia and hemodialysis

scientific article

Protein PEGylation decreases observed target association rates via a dual blocking mechanism

scientific article published on 12 August 2005

Protein folding in the periplasm of Escherichia coli

scientific article published on June 1994

Protein interference applications in cellular and developmental biology using DARPins that recognize GFP and mCherry.

scientific article

Protein tag-mediated conjugation of oligonucleotides to recombinant affinity binders for proximity ligation

scientific article

Protein-fold evolution in the test tube

scientific article

ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome

article

PtdIns(4,5)P stabilizes active states of GPCRs and enhances selectivity of G-protein coupling

scientific article published in Nature

Rapid Selection of High-Affinity Antibody scFv Fragments Using Ribosome Display

article published in 2018

Rapid selection of high-affinity binders using ribosome display

scientific article

Rapid selection of specific MAP kinase-binders from designed ankyrin repeat protein libraries

scientific article published on 21 March 2006

Receptor-Targeted Nipah Virus Glycoproteins Improve Cell-Type Selective Gene Delivery and Reveal a Preference for Membrane-Proximal Cell Attachment

scientific article

Receptor-targeted lentiviral vectors are exceptionally sensitive toward the biophysical properties of the displayed single-chain Fv.

scientific article

Reengineering anthrax toxin protective antigen for improved receptor-specific protein delivery

scientific article published on 13 August 2020

Refined crystal structure of a recombinant immunoglobulin domain and a complementarity-determining region 1-grafted mutant

scientific article published on June 1992

Removal of the conserved disulfide bridges from the scfv fragment of an antibody: effects on folding kinetics and aggregation

Reproducing the natural evolution of protein structural features with the selectively infective phage (SIP) technology. The kink in the first strand of antibody kappa domains.

scientific article

Reprogramming Bacteriophage Host Range through Structure-Guided Design of Chimeric Receptor Binding Proteins

scientific article published on 01 October 2019

Residue-Resolved Stability of Full-Consensus Ankyrin Repeat Proteins Probed by NMR

Ribosome Display: A Perspective ⬇️

scientific article published on January 1, 2012

Ribosome display efficiently selects and evolves high-affinity antibodies in vitro from immune libraries.

scientific article

Ribosome display of mammalian receptor domains

scientific article published on 2 June 2005

Ribosome display: a technology for selecting and evolving proteins from large libraries

scientific article

Ribosome display: selecting and evolving proteins in vitro that specifically bind to a target

scientific article

Rigid fusions of designed helical repeat binding proteins efficiently protect a binding surface from crystal contacts

scientific article published on 07 November 2019

Rigidity of the extracellular part of HER2: Evidence from engineering subdomain interfaces and shared-helix DARPin-DARPin fusions

scientific article published on 22 June 2017

Rigidly connected multispecific artificial binders with adjustable geometries.

scientific article

Role of monomeric activators in cobra venom phospholipase A2 action

scientific article published on 01 April 1982

Rotational symmetry of the structured Chip/LDB-SSDP core module of the Wnt enhanceosome

scientific article published on 30 September 2019

SPRi-MALDI MS: characterization and identification of a kinase from cell lysate by specific interaction with different designed ankyrin repeat proteins

scientific article

Salmonella-based platform for efficient delivery of functional binding proteins to the cytosol

scientific article published on 03 July 2020

Secretion and in vivo folding of the Fab fragment of the antibody McPC603 in Escherichia coli: influence of disulphides and cis-prolines ⬇️

scientific article published on December 1, 1991

Secretion of heterologous proteins in Escherichia coli.

scientific article

Segmental isotopic labeling by asparaginyl endopeptidase-mediated protein ligation

scientific article published on 13 March 2018

Selecting proteins with improved stability by a phage-based method ⬇️

scientific article published on October 1, 1998

Selection and characterization of DARPins specific for the neurotensin receptor 1

scientific article

Selection and characterization of Her2 binding-designed ankyrin repeat proteins

scientific article published on 8 September 2006

Selection based on the folding properties of proteins with ribosome display

scientific article

Selection for improved protein stability by phage display.

scientific article

Selection, characterization and x-ray structure of anti-ampicillin single-chain Fv fragments from phage-displayed murine antibody libraries

scientific article

Selectively infective phage (SIP) technology: scope and limitations.

scientific article

Self-immobilizing recombinant antibody fragments for immunoaffinity chromatography: generic, parallel, and scalable protein purification

scientific article published in March 2002

Sequence statistics reliably predict stabilizing mutations in a protein domain

Short-chain phosphatidylethanolamines: physical properties and susceptibility of the monomers to phospholipase A2 action

scientific article published in 1985

Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display

scientific article

Single-molecule spectroscopy of protein conformational dynamics in live eukaryotic cells

article

Site-Selective Enzymatic Labeling of Designed Ankyrin Repeat Proteins Using Protein Farnesyltransferase

scientific article published on 01 January 2019

Spontaneous self-assembly of engineered armadillo repeat protein fragments into a folded structure

scientific article

Stability improvement of antibodies for extracellular and intracellular applications: CDR grafting to stable frameworks and structure-based framework engineering

scientific article published in October 2004

Stabilization and humanization of a single-chain Fv antibody fragment specific for human lymphocyte antigen CD19 by designed point mutations and CDR-grafting onto a human framework

scientific article published in February 2009

Stabilizing ionic interactions in a full-consensus ankyrin repeat protein

scientific article

Stabilizing membrane proteins through protein engineering

scientific article published on 29 April 2013

Stepwise unfolding of ankyrin repeats in a single protein revealed by atomic force microscopy

scientific article

Strategies for selection from protein libraries composed of de novo designed secondary structure modules.

scientific article published in February 2004

Structural Basis for the Selective Inhibition of c-Jun N-Terminal Kinase 1 Determined by Rigid DARPin-DARPin Fusions

scientific article published on 7 November 2017

Structural Changes of theEscherichia coliGroEL–GroES Chaperonins upon Complex Formation in Solution: A Neutron Small Angle Scattering Study

scientific article published on 01 January 1998

Structural analysis of biological targets by host:guest crystal lattice engineering

scientific article published on 23 October 2019

Structural analysis of mycobacterial and murine hsp60 epitopes in complex with the class I MHC molecule H-2Db

scientific article (publication date: 22 May 2003)

Structural and Dynamic Properties of the Fv Fragment and the Single-Chain Fv Fragment of an Antibody in Solution Investigated by Heteronuclear 3D NMR Spectroscopy

scientific article published on 01 March 1994

Structural and functional analysis of phosphorylation-specific binders of the kinase ERK from designed ankyrin repeat protein libraries

scientific article

Structural basis for eliciting a cytotoxic effect in HER2-overexpressing cancer cells via binding to the extracellular domain of HER2

scientific article

Structural basis of adenylyl cyclase 9 activation

scientific article published on 24 February 2022

Structural determinants for improved stability of designed ankyrin repeat proteins with a redesigned C-capping module

scientific article

Structural features of the McPC603 Fab fragment not defined in the X-ray structure.

scientific article

Structural model for the interaction of a designed Ankyrin Repeat Protein with the human epidermal growth factor receptor 2.

scientific article

Structure and Energetic Contributions of a Designed Modular Peptide-Binding Protein with Picomolar Affinity

scientific article

Structure of a kinesin-tubulin complex and implications for kinesin motility

scientific article

Structure of cyclized green fluorescent protein

scientific article

Structure of signaling-competent neurotensin receptor 1 obtained by directed evolution in Escherichia coli

scientific article

Structure of the recombinant antibody Fab fragment f3p4

scientific article

Structure-Guided Design of a Peptide Lock for Modular Peptide Binders

scientific article published on 13 February 2020

Structure-based improvement of the biophysical properties of immunoglobulin VH domains with a generalizable approach

scientific article

Structure-based optimization of designed Armadillo-repeat proteins

scientific article

Structures of designed armadillo repeat proteins binding to peptides fused to globular domains

scientific article published on 10 July 2017

Systemic analysis of tyrosine kinase signaling reveals a common adaptive response program in a HER2-positive breast cancer

scientific article published on 22 January 2019

Tandem immobilized metal-ion affinity chromatography/immunoaffinity purification of His-tagged proteins--evaluation of two anti-His-tag monoclonal antibodies

scientific article published in May 1998

Targeted delivery and endosomal cellular uptake of DARPin-siRNA bioconjugates: Influence of linker stability on gene silencing

scientific article published on 16 May 2019

Tetravalent miniantibodies with high avidity assembling in Escherichia coli.

scientific article

The Antibody Society's antibody validation webinar series

scientific article published on 01 January 2020

The Effect of Folding Catalysts on the In Vivo Folding Process of Different Antibody Fragments Expressed in Escherichia coli

scientific article published on 01 January 1993

The ErbB4 CYT2 variant protects EGFR from ligand-induced degradation to enhance cancer cell motility

scientific article

The INNs and outs of antibody nonproprietary names

scientific article published in January 2016

The RGD-binding integrins αvβ6 and αvβ8 are receptors for mouse adenovirus-1 and -3 infection

scientific article published on 15 December 2021

The Rationality of Random Screening—Efficient Methods of Selection of Peptides and Oligonucleotide Ligands

scholarly article by Andreas Plückthun & Liming Ge published March 1991 in Angewandte Chemie International Edition

The design of evolution and the evolution of design

article by Andreas Plückthun & Stephen L. Mayo published August 2007 in Current Opinion in Structural Biology

The disulfide bonds in antibody variable domains: effects on stability, folding in vitro, and functional expression in Escherichia coli.

scientific article published in February 1992

The first constant domain (CH1 and CL) of an antibody used as heterodimerization domain for bispecific miniantibodies

scientific article published on 01 January 1998

The functional expression of antibody Fv fragments in Escherichia coli: improved vectors and a generally applicable purification technique.

scientific article published in March 1991

The hierarchy of mutations influencing the folding of antibody domains in Escherichia coli

scientific article published on 01 July 1999

The importance of framework residues H6, H7 and H10 in antibody heavy chains: experimental evidence for a new structural subclassification of antibody V(H) domains

scientific article

The influence of the buried glutamine or glutamate residue in position 6 on the structure of immunoglobulin variable domains

scientific article published in June 2001

The influence of the framework core residues on the biophysical properties of immunoglobulin heavy chain variable domains

scientific article published on 10 January 2009

The membrane attack complex of complement and its precursor proteins lack phospholipase activity

scientific article published on 01 April 1983

The nature of antibody heavy chain residue H6 strongly influences the stability of a VH domain lacking the disulfide bridge

The periplasmic Escherichia coli peptidylprolyl cis,trans-isomerase FkpA. I. Increased functional expression of antibody fragments with and without cis-prolines.

scientific article

The periplasmic Escherichia coli peptidylprolyl cis,trans-isomerase FkpA. II. Isomerase-independent chaperone activity in vitro

scientific article published in Journal of Biological Chemistry

The rate-limiting steps for the folding of an antibody scFv fragment

scientific article published on 01 November 1997

The structural basis of phage display elucidated by the crystal structure of the N-terminal domains of g3p

scientific article

Thermodynamic partitioning model for hydrophobic binding of polypeptides by GroEL. I. GroEL recognizes the signal sequences of beta-lactamase precursor.

scientific article

Thermodynamic partitioning model for hydrophobic binding of polypeptides by GroEL. II. GroEL recognizes thermally unfolded mature beta-lactamase.

scientific article

Transfer of engineered biophysical properties between different antibody formats and expression systems

scientific article

Transmission electron microscopy of GroEL, GroES, and the symmetrical GroEL/ES complex.

scientific article

Trapped! A Critical Evaluation of Methods for Measuring Total Cellular Uptake versus Cytosolic Localization

scientific article published on 05 April 2019

Turnover-based in vitro selection and evolution of biocatalysts from a fully synthetic antibody library.

scientific article

Two conformational states of β-lactamase bound to GroEL: a biophysical characterization 1 1Edited by A. R. Fersht

article by Pietro Gervasoni et al published January 1998 in Journal of Molecular Biology

Two-dimensional crystals of the molecular chaperone GroEL reveal structural plasticity.

scientific article

Unravelling Receptor and RGD Motif Dependence of Retargeted Adenoviral Vectors using Advanced Tumor Model Systems

scientific article published on 06 December 2019

Viral escape at the molecular level explained by quantitative T-cell receptor/peptide/MHC interactions and the crystal structure of a peptide/MHC complex

scientific article

Wege zu neuen Enzymen: Protein Engineering und Katalytische Antikörper

Yet Another Numbering Scheme for Immunoglobulin Variable Domains: An Automatic Modeling and Analysis Tool

article

Zooming in on the hydrophobic ridge of H-2D(b): implications for the conformational variability of bound peptides

scientific article

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