List of works - Johanna Myllyharju

Collagens, modifying enzymes and their mutations in humans, flies and worms

scientific article

Characterization of the human prolyl 4-hydroxylases that modify the hypoxia-inducible factor

scientific journal article

Inhibition of hypoxia-inducible factor (HIF) hydroxylases by citric acid cycle intermediates: possible links between cell metabolism and stabilization of HIF.

scientific article

Catalytic properties of the asparaginyl hydroxylase (FIH) in the oxygen sensing pathway are distinct from those of its prolyl 4-hydroxylases

scientific journal article

Prolyl 4-hydroxylases, the key enzymes of collagen biosynthesis

scientific article published on March 1, 2003

Prolyl 4-hydroxylation regulates Argonaute 2 stability

scientific article

Lysyl oxidase is essential for normal development and function of the respiratory system and for the integrity of elastic and collagen fibers in various tissues

scientific article

Oxygen-dependent ATF-4 stability is mediated by the PHD3 oxygen sensor

scientific article published on 07 August 2007

Prolyl 4-hydroxylases, key enzymes in the synthesis of collagens and regulation of the response to hypoxia, and their roles as treatment targets

scientific article published on January 2008

Effect of desferrioxamine and metals on the hydroxylases in the oxygen sensing pathway

scientific article published on 7 June 2005

Roles of the human hypoxia-inducible factor (HIF)-3α variants in the hypoxia response

scientific article published on April 10, 2011

Collagen binding specificity of the discoidin domain receptors: binding sites on collagens II and III and molecular determinants for collagen IV recognition by DDR1

scientific article published on October 28, 2010

A selective inhibitor and probe of the cellular functions of Jumonji C domain-containing histone demethylases

scientific article

The length of peptide substrates has a marked effect on hydroxylation by the hypoxia-inducible factor prolyl 4-hydroxylases

scientific article

An endoplasmic reticulum transmembrane prolyl 4-hydroxylase is induced by hypoxia and acts on hypoxia-inducible factor alpha

scientific article published on 27 August 2007

Cloning and characterization of a low molecular weight prolyl 4-hydroxylase from Arabidopsis thaliana. Effective hydroxylation of proline-rich, collagen-like, and hypoxia-inducible transcription factor alpha-like peptides

scientific article published on 25 April 2002

Identification and characterization of a third human, rat, and mouse collagen prolyl 4-hydroxylase isoenzyme

scientific journal article

Hearts of Hypoxia-inducible Factor Prolyl 4-Hydroxylase-2 Hypomorphic Mice Show Protection against Acute Ischemia-Reperfusion Injury

scientific article published on February 25, 2010

High myopia caused by a mutation in LEPREL1, encoding prolyl 3-hydroxylase 2

scientific article

Premature aggregation of type IV collagen and early lethality in lysyl hydroxylase 3 null mice

scholarly article

Characterization of a second Arabidopsis thaliana prolyl 4-hydroxylase with distinct substrate specificity

scientific article

Hypoxia-inducible factor (HIF)-3alpha is subject to extensive alternative splicing in human tissues and cancer cells and is regulated by HIF-1 but not HIF-2.

scientific article published on 21 April 2010

The primary substrate binding site in the b' domain of ERp57 is adapted for endoplasmic reticulum lectin association

scientific article published on 10 February 2004

Characterization of recombinant human prolyl 3-hydroxylase isoenzyme 2, an enzyme modifying the basement membrane collagen IV

scientific article

Loss of assembly of the main basement membrane collagen, type IV, but not fibril-forming collagens and embryonic death in collagen prolyl 4-hydroxylase I null mice

scientific journal article

Lysyl oxidase oxidizes cell membrane proteins and enhances the chemotactic response of vascular smooth muscle cells

scientific article published on 27 June 2008

Transmembrane prolyl 4-hydroxylase is a fourth prolyl 4-hydroxylase regulating EPO production and erythropoiesis

scientific article published on September 5, 2012

Chlamydomonas reinhardtii has multiple prolyl 4-hydroxylases, one of which is essential for proper cell wall assembly

scientific article published on 12 January 2007

Characterization of three fragments that constitute the monomers of the human lysyl hydroxylase isoenzymes 1-3. The 30-kDa N-terminal fragment is not required for lysyl hydroxylase activity

scientific article

Extracellular matrix genes as hypoxia-inducible targets

scientific article

Lysyl hydroxylase 2 is a specific telopeptide hydroxylase, while all three isoenzymes hydroxylate collagenous sequences

scientific article published on 16 January 2007

HIF prolyl 4-hydroxylases and their potential as drug targets

scientific article published on January 2009

The Crystal Structure of an Algal Prolyl 4-Hydroxylase Complexed with a Proline-rich Peptide Reveals a Novel Buried Tripeptide Binding Motif

scientific article

The exoskeleton collagens in Caenorhabditis elegans are modified by prolyl 4-hydroxylases with unique combinations of subunits

scientific article

Tissue-specific changes in the hydroxylysine content and cross-links of collagens and alterations in fibril morphology in lysyl hydroxylase 1 knock-out mice

scientific article published on 29 December 2006

Many amino acid substitutions in a hypoxia-inducible transcription factor (HIF)-1alpha-like peptide cause only minor changes in its hydroxylation by the HIF prolyl 4-hydroxylases: substitution of 3,4-dehydroproline or azetidine-2-carboxylic acid for

scientific article published on 12 October 2004

Hypoxia-inducible factor prolyl 4-hydroxylases: common and specific roles

scientific article published on April 1, 2013

HIF Prolyl 4-Hydroxylase-2 Inhibition Improves Glucose and Lipid Metabolism and Protects Against Obesity and Metabolic Dysfunction

scientific article published on 01 May 2014

The Ras signaling inhibitor LOX-PP interacts with Hsp70 and c-Raf to reduce Erk activation and transformed phenotype of breast cancer cells

scientific article

Three binding sites in protein-disulfide isomerase cooperate in collagen prolyl 4-hydroxylase tetramer assembly

scientific article

Hypoxia-inducible factor-1 (HIF-1) but not HIF-2 is essential for hypoxic induction of collagen prolyl 4-hydroxylases in primary newborn mouse epiphyseal growth plate chondrocytes.

scientific article

The active site of an algal prolyl 4-hydroxylase has a large structural plasticity

scientific article

Identification and characterization of structural domains of human ERp57: association with calreticulin requires several domains

scientific article published on 19 January 2004

T-cell activation leads to reduced collagen maturation in atherosclerotic plaques of Apoe(-/-) mice

scientific article

Activation of hypoxia response in endothelial cells contributes to ischemic cardioprotection

scientific article published on 17 June 2013

The peptide-substrate-binding domain of human collagen prolyl 4-hydroxylases. Backbone assignments, secondary structure, and binding of proline-rich peptides

scientific article

Missense mutations that cause Bruck syndrome affect enzymatic activity, folding, and oligomerization of lysyl hydroxylase 2

scientific article published on 17 September 2009

PHD1 regulates p53-mediated colorectal cancer chemoresistance.

scientific article published on 19 August 2015

Assembly of stable human type I and III collagen molecules from hydroxylated recombinant chains in the yeast Pichia pastoris. Effect of an engineered C-terminal oligomerization domain foldon

scientific article

CD146(+) cells are essential for kidney vasculature development.

scientific article published on 18 April 2016

List of works by Johanna Myllyharju

Collagens, modifying enzymes and their mutations in humans, flies and worms

Characterization of the human prolyl 4-hydroxylases that modify the hypoxia-inducible factor

Inhibition of hypoxia-inducible factor (HIF) hydroxylases by citric acid cycle intermediates: possible links between cell metabolism and stabilization of HIF.

Catalytic properties of the asparaginyl hydroxylase (FIH) in the oxygen sensing pathway are distinct from those of its prolyl 4-hydroxylases

Prolyl 4-hydroxylases, the key enzymes of collagen biosynthesis

Prolyl 4-hydroxylation regulates Argonaute 2 stability

Lysyl oxidase is essential for normal development and function of the respiratory system and for the integrity of elastic and collagen fibers in various tissues

Oxygen-dependent ATF-4 stability is mediated by the PHD3 oxygen sensor

Prolyl 4-hydroxylases, key enzymes in the synthesis of collagens and regulation of the response to hypoxia, and their roles as treatment targets

Effect of desferrioxamine and metals on the hydroxylases in the oxygen sensing pathway

Roles of the human hypoxia-inducible factor (HIF)-3α variants in the hypoxia response

Collagen binding specificity of the discoidin domain receptors: binding sites on collagens II and III and molecular determinants for collagen IV recognition by DDR1

A selective inhibitor and probe of the cellular functions of Jumonji C domain-containing histone demethylases

The length of peptide substrates has a marked effect on hydroxylation by the hypoxia-inducible factor prolyl 4-hydroxylases

An endoplasmic reticulum transmembrane prolyl 4-hydroxylase is induced by hypoxia and acts on hypoxia-inducible factor alpha

Cloning and characterization of a low molecular weight prolyl 4-hydroxylase from Arabidopsis thaliana. Effective hydroxylation of proline-rich, collagen-like, and hypoxia-inducible transcription factor alpha-like peptides

Identification and characterization of a third human, rat, and mouse collagen prolyl 4-hydroxylase isoenzyme

Hearts of Hypoxia-inducible Factor Prolyl 4-Hydroxylase-2 Hypomorphic Mice Show Protection against Acute Ischemia-Reperfusion Injury

High myopia caused by a mutation in LEPREL1, encoding prolyl 3-hydroxylase 2

Premature aggregation of type IV collagen and early lethality in lysyl hydroxylase 3 null mice

Characterization of a second Arabidopsis thaliana prolyl 4-hydroxylase with distinct substrate specificity

Hypoxia-inducible factor (HIF)-3alpha is subject to extensive alternative splicing in human tissues and cancer cells and is regulated by HIF-1 but not HIF-2.

The primary substrate binding site in the b' domain of ERp57 is adapted for endoplasmic reticulum lectin association

Characterization of recombinant human prolyl 3-hydroxylase isoenzyme 2, an enzyme modifying the basement membrane collagen IV

Loss of assembly of the main basement membrane collagen, type IV, but not fibril-forming collagens and embryonic death in collagen prolyl 4-hydroxylase I null mice

Lysyl oxidase oxidizes cell membrane proteins and enhances the chemotactic response of vascular smooth muscle cells

Transmembrane prolyl 4-hydroxylase is a fourth prolyl 4-hydroxylase regulating EPO production and erythropoiesis

Chlamydomonas reinhardtii has multiple prolyl 4-hydroxylases, one of which is essential for proper cell wall assembly

Characterization of three fragments that constitute the monomers of the human lysyl hydroxylase isoenzymes 1-3. The 30-kDa N-terminal fragment is not required for lysyl hydroxylase activity

Extracellular matrix genes as hypoxia-inducible targets

Lysyl hydroxylase 2 is a specific telopeptide hydroxylase, while all three isoenzymes hydroxylate collagenous sequences

HIF prolyl 4-hydroxylases and their potential as drug targets

The Crystal Structure of an Algal Prolyl 4-Hydroxylase Complexed with a Proline-rich Peptide Reveals a Novel Buried Tripeptide Binding Motif

The exoskeleton collagens in Caenorhabditis elegans are modified by prolyl 4-hydroxylases with unique combinations of subunits

Tissue-specific changes in the hydroxylysine content and cross-links of collagens and alterations in fibril morphology in lysyl hydroxylase 1 knock-out mice

Many amino acid substitutions in a hypoxia-inducible transcription factor (HIF)-1alpha-like peptide cause only minor changes in its hydroxylation by the HIF prolyl 4-hydroxylases: substitution of 3,4-dehydroproline or azetidine-2-carboxylic acid for

Hypoxia-inducible factor prolyl 4-hydroxylases: common and specific roles

HIF Prolyl 4-Hydroxylase-2 Inhibition Improves Glucose and Lipid Metabolism and Protects Against Obesity and Metabolic Dysfunction

The Ras signaling inhibitor LOX-PP interacts with Hsp70 and c-Raf to reduce Erk activation and transformed phenotype of breast cancer cells

Three binding sites in protein-disulfide isomerase cooperate in collagen prolyl 4-hydroxylase tetramer assembly

Hypoxia-inducible factor-1 (HIF-1) but not HIF-2 is essential for hypoxic induction of collagen prolyl 4-hydroxylases in primary newborn mouse epiphyseal growth plate chondrocytes.

The active site of an algal prolyl 4-hydroxylase has a large structural plasticity

Identification and characterization of structural domains of human ERp57: association with calreticulin requires several domains

T-cell activation leads to reduced collagen maturation in atherosclerotic plaques of Apoe(-/-) mice

Activation of hypoxia response in endothelial cells contributes to ischemic cardioprotection

The peptide-substrate-binding domain of human collagen prolyl 4-hydroxylases. Backbone assignments, secondary structure, and binding of proline-rich peptides

Missense mutations that cause Bruck syndrome affect enzymatic activity, folding, and oligomerization of lysyl hydroxylase 2

PHD1 regulates p53-mediated colorectal cancer chemoresistance.

Assembly of stable human type I and III collagen molecules from hydroxylated recombinant chains in the yeast Pichia pastoris. Effect of an engineered C-terminal oligomerization domain foldon

CD146(+) cells are essential for kidney vasculature development.