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List of works by Peter Leadlay

A bacterial calcium-binding protein homologous to calmodulin

scientific article published in September 1987

A chain initiation factor common to both modular and aromatic polyketide synthases

scientific article published in Nature

A common origin for guanidinobutanoate starter units in antifungal natural products

scientific article published on 11 November 2013

A defined system for hybrid macrolide biosynthesis in Saccharopolyspora erythraea

scientific article published in April 2000

A late-stage intermediate in salinomycin biosynthesis is revealed by specific mutation in the biosynthetic gene cluster.

scientific article

A new modular polyketide synthase in the erythromycin producer Saccharopolyspora erythraea.

scientific article

A novel mycolactone from a clinical isolate of Mycobacterium ulcerans provides evidence for additional toxin heterogeneity as a result of specific changes in the modular polyketide synthase

scientific article

A novel mycolactone toxin obtained by biosynthetic engineering

scientific article

A repeated decapeptide motif in the C-terminal domain of the ribosomal RNA methyltransferase from the erythromycin producer Saccharopolyspora erythraea

scientific article published in March 1990

A small, discrete acyl carrier protein is involved in de novo fatty acid biosynthesis in Streptomyces erythraeus.

scientific article

Accumulation of an E,E,E-triene by the monensin-producing polyketide synthase when oxidative cyclization is blocked

scientific article

Actinomycete integrative and conjugative pMEA-like elements of Amycolatopsis and Saccharopolyspora decoded

scientific article published on 03 March 2008

An additional dehydratase-like activity is required for lankacidin antibiotic biosynthesis

scientific article published on 27 September 2011

Analysis of eryBI, eryBIII and eryBVII from the erythromycin biosynthetic gene cluster in Saccharopolyspora erythraea

scientific article published in April 1998

Analysis of seven genes from the eryAI-eryK region of the erythromycin biosynthetic gene cluster in Saccharopolyspora erythraea

scientific article published on October 1997

Analysis of the biosynthetic gene cluster for the polyether antibiotic monensin in Streptomyces cinnamonensis and evidence for the role of monB and monC genes in oxidative cyclization

scientific article published in September 2003

Analysis of the tetronomycin gene cluster: insights into the biosynthesis of a polyether tetronate antibiotic

scientific article

Biosynthesis of the angiogenesis inhibitor borrelidin by Streptomyces parvulus Tü4055: insights into nitrile formation

scientific article

Biosynthesis of the angiogenesis inhibitor borrelidin: directed biosynthesis of novel analogues

scientific article published on 11 May 2006

Biosynthesis of the immunosuppressants FK506, FK520, and rapamycin involves a previously undescribed family of enzymes acting on chorismate

scientific article

Biosynthesis of the putative siderophore erythrochelin requires unprecedented crosstalk between separate nonribosomal peptide gene clusters

scientific article

Biosynthetic engineering of polyketide synthases

Biosynthetic gene cluster of the glycopeptide antibiotic teicoplanin: characterization of two glycosyltransferases and the key acyltransferase

scientific article published on January 2004

Borrelidin modulates the alternative splicing of VEGF in favour of anti-angiogenic isoforms

scientific article

Catalytically active tetramodular 6-deoxyerythonolide B synthase fusion proteins

scientific article published in November 2003

Chain initiation on the soraphen-producing modular polyketide synthase from Sorangium cellulosum

scientific article published in December 2001

Chain initiation on type I modular polyketide synthases revealed by limited proteolysis and ion-trap mass spectrometry

scientific article published on 01 May 2005

Chemical probes for the functionalization of polyketide intermediates

scientific article

Cloning and sequence analysis of genes involved in erythromycin biosynthesis in Saccharopolyspora erythraea: sequence similarities between EryG and a family of S-adenosylmethionine-dependent methyltransferases

scientific article published on November 1991

Combinatorial biosynthesis of reduced polyketides

scientific article

Common evolutionary origin for the unstable virulence plasmid pMUM found in geographically diverse strains of Mycobacterium ulcerans

scientific article

Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338. ⬇️

scientific article published on 18 March 2007

Construction of new vectors for high-level expression in actinomycetes

scientific article published in August 1998

Covalent linkage mediates communication between ACP and TE domains in modular polyketide synthases

scientific article

Deciphering the genetic basis for polyketide variation among mycobacteria producing mycolactones

scientific article

Delineating the biosynthesis of gentamicin x2, the common precursor of the gentamicin C antibiotic complex

scientific article

Direct production of ivermectin-like drugs after domain exchange in the avermectin polyketide synthase of Streptomyces avermitilis ATCC31272.

scientific article published in August 2003

Directed mutagenesis alters the stereochemistry of catalysis by isolated ketoreductase domains from the erythromycin polyketide synthase

scientific article

Divergent sequence motifs correlated with the substrate specificity of (methyl)malonyl-CoA:acyl carrier protein transacylase domains in modular polyketide synthases

article

Engineered biosynthesis of hybrid macrolide polyketides containing D-angolosamine and D-mycaminose moieties

scientific article

Engineered biosynthesis of phenyl-substituted polyketides

scientific article published in August 2004

Engineering a polyketide with a longer chain by insertion of an extra module into the erythromycin-producing polyketide synthase

scientific article published on 01 May 2001

Engineering broader specificity into an antibiotic-producing polyketide synthase

scientific article published in January 1998

Engineering of the spinosyn PKS: directing starter unit incorporation

scientific article published in December 2006

Engineering specificity of starter unit selection by the erythromycin-producing polyketide synthase

scientific article published in March 2002

Enzyme assembly line pictured

scientific article published in Nature

Enzymology of Pyran Ring A Formation in Salinomycin Biosynthesis

scholarly article by Hanna Luhavaya et al published 9 November 2015 in Angewandte Chemie International Edition

Evaluating Ketoreductase Exchanges as a Means of Rationally Altering Polyketide Stereochemistry

scientific article

Evidence for a double-helical structure for modular polyketide synthases.

scientific article published on February 1996

Evidence for the Role of the monB Genes in Polyether Ring Formation during Monensin Biosynthesis

article

Evidence that a novel thioesterase is responsible for polyketide chain release during biosynthesis of the polyether ionophore monensin.

scientific article

Free (S,S,)-diaminopimelate is not an obligatory intermediate in lysine biosynthesis inCorynebacterium glutamicum ⬇️

scientific article published on February 15, 1979

Giant plasmid-encoded polyketide synthases produce the macrolide toxin of Mycobacterium ulcerans ⬇️

scientific article

Glyceryl-S-acyl carrier protein as an intermediate in the biosynthesis of tetronate antibiotics

scientific article published in January 2008

Heterologous expression in Saccharopolyspora erythraea of a pentaketide synthase derived from the spinosyn polyketide synthase.

scientific article published on 29 October 2003

Hidden Specificities in Enzyme Catalysis: Structural Basis of Substrate Structure-Selectivity Relationship of a Ketoreductase

scientific article published on 01 March 2019

High-throughput mutagenesis to evaluate models of stereochemical control in ketoreductase domains from the erythromycin polyketide synthase

scientific article

Identification of DEBS 1, DEBS 2 and DEBS 3, the multienzyme polypeptides of the erythromycin-producing polyketide synthase from Saccharopolyspora erythraea

scientific article published on June 1992

Identification of a phosphopantetheinyl transferase for erythromycin biosynthesis in Saccharopolyspora erythraea.

scientific article published in January 2004

In vitro reconstruction of tetronate RK-682 biosynthesis

scientific article published on 20 December 2009

In vivo trapping of polyketide intermediates from an assembly line synthase using malonyl carba(dethia)-N-acetyl cysteamines

scientific article published on 8 February 2011

Induction, purification and characterisation of acyl-ACP thioesterase from developing seeds of oil seed rape (Brassica napus).

scientific article

Insights into lasalocid A ring formation by chemical chain termination in vivo

scientific article published on 24 October 2011

Insights into polyether biosynthesis from analysis of the nigericin biosynthetic gene cluster in Streptomyces sp. DSM4137.

scientific article published on June 2007

Insights into the stereospecificity of ketoreduction in a modular polyketide synthase

scientific article published on 21 February 2011

Intermediates in monensin biosynthesis: A late step in biosynthesis of the polyether ionophore monensin is crucial for the integrity of cation binding

scientific article published on 10 February 2014

Intermediates released from a polyether-producing polyketide synthase provide insight into the mechanism of oxidative cyclization

scientific article published in September 2003

Isolation and characterization of pre-rapamycin, the first macrocyclic intermediate in the biosynthesis of the immunosuppressant rapamycin by S. hygroscopicus

scientific article

Iterative Mechanism of Macrodiolide Formation in the Anticancer Compound Conglobatin.

scientific article published on June 2015

Knowledge-based design of bimodular and trimodular polyketide synthases based on domain and module swaps: a route to simple statin analogues

scientific article published in October 1999

Macrodiolide formation by the thioesterase of a modular polyketide synthase.

scientific article

Mammalian fatty acid synthase: closure on a textbook mechanism?

scientific article published in February 2003

Modification of enoyl-CoA hydratase using diethyl pyrocarbonate

scientific article published on August 1981

Molecular Basis of Celmer's Rules: Stereochemistry of Catalysis by Isolated Ketoreductase Domains from Modular Polyketide Synthases

scientific article published on 01 October 2005

Molecular basis of Celmer’s rules: role of the ketosynthase domain in epimerisation and demonstration that ketoreductase domains can have altered product specificity with unnatural substrates

scientific article published on 01 April 2001

Molecular basis of celmer's rules: the role of two ketoreductase domains in the control of chirality by the erythromycin modular polyketide synthase

scientific article published on 01 April 1999

Mutagenesis of a modular polyketide synthase enoylreductase domain reveals insights into catalysis and stereospecificity

scientific article

Mutasynthesis of rapamycin analogues through the manipulation of a gene governing starter unit biosynthesis.

scientific article published in July 2005

Mycolactone activation of Wiskott-Aldrich syndrome proteins underpins Buruli ulcer formation

scientific article published on 15 March 2013

Mycolactone diffuses from Mycobacterium ulcerans-infected tissues and targets mononuclear cells in peripheral blood and lymphoid organs

scientific article published in 2008

Mycolactones: immunosuppressive and cytotoxic polyketides produced by aquatic mycobacteria

scientific article published on 17 April 2008

New erythromycin derivatives from Saccharopolyspora erythraea using sugar O-methyltransferases from the spinosyn biosynthetic gene cluster

scientific article

New rapamycin derivatives by precursor-directed biosynthesis

scientific article

Novel octaketide macrolides related to 6-deoxyerythronolide B provide evidence for iterative operation of the erythromycin polyketide synthase

scientific article published on 01 February 2000

Obituary: Jonathan B. Spencer (1960-2008).

scientific article

Oligonucleotide probes for bacterial acylcarrier protein genes

scientific article

Organisation of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: analysis of genes flanking the polyketide synthase

scientific article published on February 1996

Organization of the biosynthetic gene cluster for the macrolide concanamycin A in Streptomyces neyagawaensis ATCC 27449.

scientific article published in October 2005

Organization of the biosynthetic gene cluster in Streptomyces sp. DSM 4137 for the novel neuroprotectant polyketide meridamycin.

scientific article published in December 2006

Origin and True Nature of the Starter Unit for the Rapamycin Polyketide Synthase We thank Dr. Bradley S. Moore for help with the deuterium NMR experiments. This work was supported by grants from The Wellcome Trust (to J.S. and P.F.L.) and from the N

scientific article published on 01 February 2001

Origin of starter units for erythromycin biosynthesis.

scientific article

Parallel pathways for oxidation of 14-membered polyketide macrolactones in Saccharopolyspora erythraea

scientific article published in May 2002

Polyketide synthesis in vitro on a modular polyketide synthase

scientific article published on 01 September 1995

Prediction and manipulation of the stereochemistry of enoylreduction in modular polyketide synthases

scientific article published in November 2008

Prokaryotic calcium-binding protein of the calmodulin superfamily Calcium binding to a Saccharopolyspora erythraea 20 kDa protein

scientific article published on 01 March 1992

Protection of radical intermediates at the active site of adenosylcobalamin-dependent methylmalonyl-CoA mutase

scientific article

Rapamycin biosynthesis: Elucidation of gene product function.

scientific article

Recent advances in the field of bioactive tetronates

scientific article

Robust reporter system based on chalcone synthase rppA gene from Saccharopolyspora erythraea.

scientific article published on 13 August 2010

Role of type II thioesterases: evidence for removal of short acyl chains produced by aberrant decarboxylation of chain extender units

scientific article published in February 2001

Separation of anti-angiogenic and cytotoxic activities of borrelidin by modification at the C17 side chain

scientific article published on 8 September 2006

Site-specific modification of the anticancer and antituberculosis polyether salinomycin by biosynthetic engineering

scientific article published on 22 August 2014

Site-specific recombination strategies for engineering actinomycete genomes

scientific article published on 13 January 2012

Skipping in a hybrid polyketide synthase. Evidence for ACP-to-ACP chain transfer

scientific article published in July 2002

Specificity and promiscuity at the branch point in gentamicin biosynthesis

scientific article published on 17 April 2014

Stabilization of radical intermediates by an active-site tyrosine residue in methylmalonyl-CoA mutase

scientific article

Stereochemistry of catalysis by the ketoreductase activity in the first extension module of the erythromycin polyketide synthase

scientific article published in February 2002

Stereoselectivity of Isolated Dehydratase Domains of the Borrelidin Polyketide Synthase: Implications for cis Double Bond Formation

scientific article published on 05 April 2011

Structural Basis for the Activity and Substrate Specificity of Fluoroacetyl-CoA Thioesterase FlK

scientific article

Structural elucidation studies of erythromycins by electrospray tandem mass spectrometry II

scientific article published on 01 January 1999

Structural elucidation studies of polyketide tetrasubstituted δ-lactones by gas chromatography/tandem mass spectrometry and electrospray mass spectrometry

scientific article published on 01 January 1999

Structure elucidation of a novel family of mycolactone toxins from the frog pathogen Mycobacterium sp. MU128FXT by mass spectrometry

scientific article

Structure of the glycosyltransferase EryCIII in complex with its activating P450 homologue EryCII

scientific article

Substrate specificity of the acyl transferase domains of EpoC from the epothilone polyketide synthase

scientific article published on 12 December 2007

Sugaring the pill by design

scientific article published on 01 January 1998

Synthesis of chiral acetic acids by a simple enzymatic procedure

scientific article published on 01 November 1982

Synthesis of complex intermediates for the study of a dehydratase from borrelidin biosynthesis

scientific article

Synthesis of methyl[3H]acetimidate of high specific radioactivity, a reagent for radiolabeling proteins

scientific article published on 01 December 1980

Synthetic chain terminators off-load intermediates from a type I polyketide synthase

scientific article published in March 2010

The Effect of Rapeseed Oil Uptake on the Production of Erythromycin and Triketide Lactone by Saccharopolyspora erythraea

scientific article published in October 1999

The Thioesterase of the Erythromycin-Producing Polyketide Synthase: Influence of Acyl Chain Structure on the Mode of Release of Substrate Analogues from the Acyl Enzyme Intermediates

scientific article published on 01 June 1998

The cell wall-associated mycolactone polyketide synthases are necessary but not sufficient for mycolactone biosynthesis ⬇️

scientific article (publication date: 2013)

The changing patterns of covalent active site occupancy during catalysis on a modular polyketide synthase multienzyme revealed by ion-trap mass spectrometry

scientific article published on 27 October 2009

The gene cluster for fluorometabolite biosynthesis in Streptomyces cattleya: a thioesterase confers resistance to fluoroacetyl-coenzyme A.

scientific article

The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233: genetic and biochemical evidence for the roles of two glycosyltransferases and a deacetylase

scientific article

The putative elaiophylin biosynthetic gene cluster in Streptomyces sp. DSM4137 is adjacent to genes encoding adenosylcobalamin-dependent methylmalonyl CoA mutase and to genes for synthesis of cobalamin

scientific article

The role of cep15 in the biosynthesis of chloroeremomycin: reactivation of an ancestral catalytic function

scientific article published in May 2008

The structure of docking domains in modular polyketide synthases

scientific article

Tritium Isotope Effects in Adenosylcobalamin-Dependent Methylmalonyl-CoA Mutase†

scientific article published on 01 September 1996

Unusual acetylation-elimination in the formation of tetronate antibiotics

scientific article published on 18 April 2013

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