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List of works by Bernd Bukau

A PDZ switch for a cellular stress response

scientific article published on May 2004

A conserved loop in the ATPase domain of the DnaK chaperone is essential for stable binding of GrpE ⬇️

scientific article published on February 1, 1994

A cycle of binding and release of the DnaK, DnaJ and GrpE chaperones regulates activity of the Escherichia coli heat shock transcription factor sigma32.

scientific article

A folding machine for many but a master of none

scientific article

A peptide deformylase-ribosome complex reveals mechanism of nascent chain processing

scientific article

A prion-like domain in Hsp42 drives chaperone-facilitated aggregation of misfolded proteins

scientific article

A tightly regulated molecular toggle controls AAA+ disaggregase

scientific article published on 18 November 2012

AAA+ proteins and substrate recognition, it all depends on their partner in crime

scientific article

Accurate prediction of cellular co-translational folding indicates proteins can switch from post- to co-translational folding

scientific article published on 18 February 2016

Adaptor protein controlled oligomerization activates the AAA+ protein ClpC

scientific article

Aggregation-induced changes in the chemical exchange saturation transfer (CEST) signals of proteins

scientific article

Aha, another regulator for hsp90 chaperones

scientific article published on 01 December 2002

Allosteric regulation of Hsp70 chaperones by a proline switch

scientific article published in February 2006

Allosteric regulation of Hsp70 chaperones involves a conserved interdomain linker

scientific article published on 19 October 2006

Alternative modes of client binding enable functional plasticity of Hsp70.

scientific article

Amide hydrogen exchange reveals conformational changes in hsp70 chaperones important for allosteric regulation

scientific article

Author Correction: Molecular dissection of amyloid disaggregation by human HSP70

scientific article published on 22 December 2020

Bacterial and Yeast AAA+ Disaggregases ClpB and Hsp104 Operate through Conserved Mechanism Involving Cooperation with Hsp70.

scientific article published on 8 September 2016

Bag-1M accelerates nucleotide release for human Hsc70 and Hsp70 and can act concentration-dependent as positive and negative cofactor.

scientific article published on 5 July 2001

Binding specificity of Escherichia coli trigger factor ⬇️

scientific article published on November 27, 2001

Broad yet high substrate specificity: the challenge of AAA+ proteins

scientific article published on April 2004

Ca2+-induced permeabilization of the Escherichia coli outer membrane: comparison of transformation and reconstitution of binding-protein-dependent transport.

scientific article

Cell Biology. Finding nascent proteins the right home

scientific article published on 01 April 2015

Cell biology. When the beginning marks the end

scientific article published on 01 February 2010

Cellular Functions and Mechanisms of Action of Small Heat Shock Proteins

scientific article published on 15 May 2019

Cellular Handling of Protein Aggregates by Disaggregation Machines

scientific article published in January 2018

Cellular defects caused by deletion of the Escherichia coli dnaK gene indicate roles for heat shock protein in normal metabolism

scientific article

Cellular sequestrases maintain basal Hsp70 capacity ensuring balanced proteostasis

scientific article published on 24 October 2019

Cellular strategies for controlling protein aggregation

scientific article

Chaperone function on Crete: a meeting report ⬇️

scientific article published on April 1, 1996

Chaperone network in the yeast cytosol: Hsp110 is revealed as an Hsp70 nucleotide exchange factor

scientific article

Chaperone networks in protein disaggregation and prion propagation

scientific article published on 10 May 2012

Chaperone-assisted folding of newly synthesized proteins in the cytosol

scientific article

Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E. coli

scientific article

Characterization of a trap mutant of the AAA+ chaperone ClpB.

scientific article

Chp1 is a dedicated chaperone at the ribosome that safeguards eEF1A biogenesis

scientific article published in 2024

ClpS is an essential component of the N-end rule pathway in Escherichia coli.

scientific article published in February 2006

ClpS is the recognition component for Escherichia coli substrates of the N-end rule degradation pathway

scientific article published on 17 March 2009

ClpS, a substrate modulator of the ClpAP machine

scientific article

Co-translational mechanisms of protein maturation.

scientific article published on 12 December 2013

Common and specific mechanisms of AAA+ proteins involved in protein quality control

scientific article published on February 2008

Compartment-specific aggregases direct distinct nuclear and cytoplasmic aggregate deposition

scientific article

Concerted action of the ribosome and the associated chaperone trigger factor confines nascent polypeptide folding

scientific article published on 23 August 2012

Conserved ATPase and luciferase refolding activities between bacteria and yeast Hsp70 chaperones and modulators.

scientific article published on July 1995

Conserved residues in the N-domain of the AAA+ chaperone ClpA regulate substrate recognition and unfolding

scientific article

Cooperation of Hsp70 and Hsp100 chaperone machines in protein disaggregation

scientific article

Cooperative and independent activities of Sgt2 and Get5 in the targeting of tail-anchored proteins

scientific article published on 28 May 2011

Coordination of translational control and protein homeostasis during severe heat stress

scientific article

Correction: Functional Analysis of Hsp70 Inhibitors.

scientific article published on 19 December 2013

Cotranslational assembly of protein complexes in eukaryotes revealed by ribosome profiling.

scientific article

Crucial HSP70 co-chaperone complex unlocks metazoan protein disaggregation

scientific article

Crystallization and preliminary X-ray analysis of the Escherichia coli adaptor protein ClpS, free and in complex with the N-terminal domain of ClpA.

scientific article

Cyanobacterial ClpC/HSP100 protein displays intrinsic chaperone activity

scientific article

DegP: a Protein "Death Star".

scientific article published in July 2008

Delta dnaK52 mutants of Escherichia coli have defects in chromosome segregation and plasmid maintenance at normal growth temperatures.

scientific article

Dimerization of the human E3 ligase CHIP via a coiled-coil domain is essential for its activity.

scientific article published on 10 November 2003

DnaK, DnaJ and GrpE form a cellular chaperone machinery capable of repairing heat-induced protein damage.

scientific article

Drosophila GAGA factor polyglutamine domains exhibit prion-like behavior

scientific article

Dynamic enzyme docking to the ribosome coordinates N-terminal processing with polypeptide folding.

scientific article published on 16 June 2013

Dynamics of trigger factor interaction with translating ribosomes

scientific article published on 28 November 2007

Escherichia coli FtsH is a membrane-bound, ATP-dependent protease which degrades the heat-shock transcription factor sigma 32.

scientific article published on June 1995

Escherichia coli trigger factor is a prolyl isomerase that associates with nascent polypeptide chains.

scientific article published on April 1996

Evolution of an intricate J-protein network driving protein disaggregation in eukaryotes.

scientific article

Functional analysis of Hsp70 inhibitors

scientific article

Functional defects of the DnaK756 mutant chaperone of Escherichia coli indicate distinct roles for amino- and carboxyl-terminal residues in substrate and co-chaperone interaction and interdomain communication.

scientific article

GPD1 Specifically Marks Dormant Glioma Stem Cells with a Distinct Metabolic Profile

scientific article published on 11 July 2019

Genetic dissection of the roles of chaperones and proteases in protein folding and degradation in the Escherichia coli cytosol

scientific article published on 01 April 2001

Getting newly synthesized proteins into shape.

scientific article

Global profiling of SRP interaction with nascent polypeptides.

scientific article published on 3 August 2016

GrpE accelerates nucleotide exchange of the molecular chaperone DnaK with an associative displacement mechanism.

scientific article

HSP40 proteins use class-specific regulation to drive HSP70 functional diversity

scientific article published on 11 November 2020

Head-to-tail interactions of the coiled-coil domains regulate ClpB activity and cooperation with Hsp70 in protein disaggregation

scientific article

Heat shock regulation in the ftsH null mutant of Escherichia coli: dissection of stability and activity control mechanisms of sigma32 in vivo

scientific article published on 01 November 1998

Hormesis enables cells to handle accumulating toxic metabolites during increased energy flux

scientific article

Hsp110 chaperones regulate prion formation and propagation in S. cerevisiae by two discrete activities

scientific article

Hsp110 is a nucleotide-activated exchange factor for Hsp70.

scientific article published on 24 January 2008

Hsp42 is required for sequestration of protein aggregates into deposition sites in Saccharomyces cerevisiae

scientific article

Hsp70 chaperone machines.

scientific article

Hsp70 chaperone systems: diversity of cellular functions and mechanism of action

scientific article published on March 1, 1998

Hsp70 displaces small heat shock proteins from aggregates to initiate protein refolding.

scientific article published on 20 February 2017

Hsp70 proteins bind Hsp100 regulatory M domains to activate AAA+ disaggregase at aggregate surfaces

scientific article published on 18 November 2012

Hsp70 targets Hsp100 chaperones to substrates for protein disaggregation and prion fragmentation

scientific article published on August 2012

Human Hsp70 Disaggregase Reverses Parkinson's-Linked α-Synuclein Amyloid Fibrils

scientific article published on 19 August 2015

Human and yeast Hsp110 chaperones exhibit functional differences

scientific article published on 9 December 2005

Identification of the prolyl isomerase domain of Escherichia coli trigger factor

scientific article published on 01 April 1996

Immunogold localization of the DnaK heat shock protein in Escherichia coli cells.

scientific article

In vivo properties of the disaggregase function of J-proteins and Hsc70 in Caenorhabditis elegans stress and aging.

scientific article published on 10 October 2017

Incomplete proteasomal degradation of green fluorescent proteins in the context of tandem fluorescent protein timers

scientific article published on 25 November 2015

Influence of GrpE on DnaK-substrate interactions

scientific article published on 21 April 2004

Insights into the structural dynamics of the Hsp110-Hsp70 interaction reveal the mechanism for nucleotide exchange activity

scholarly article

Integrating protein homeostasis strategies in prokaryotes

scientific article published on April 2011

Interactions between nascent proteins translated by adjacent ribosomes drive homomer assembly

scientific article published on 01 January 2021

Investigation of the interaction between DnaK and DnaJ by surface plasmon resonance spectroscopy.

scientific article published in June 1999

Its substrate specificity characterizes the DnaJ co-chaperone as a scanning factor for the DnaK chaperone.

scientific article

L23 protein functions as a chaperone docking site on the ribosome

scientific article published in September 2002

Large-scale purification of ribosome-nascent chain complexes for biochemical and structural studies

scientific article

Low temperature of GroEL/ES overproduction permits growth of Escherichia coli cells lacking trigger factor DnaK

scientific article published on 01 June 2005

Low temperature or GroEL/ES overproduction permits growth of Escherichia coli cells lacking trigger factor and DnaK.

scientific article published in February 2004

M domains couple the ClpB threading motor with the DnaK chaperone activity

scientific article published in January 2007

Mapping temperature-induced conformational changes in the Escherichia coli heat shock transcription factor sigma 32 by amide hydrogen exchange.

scientific article

MecA, an adaptor protein necessary for ClpC chaperone activity

scholarly article

Mechanics of Hsp70 chaperones enables differential interaction with client proteins

scientific article published on 30 January 2011

Mechanism of Hsp104/ClpB inhibition by prion curing Guanidinium hydrochloride

scientific article published on 14 February 2013

Mechanism of substrate recognition by Hsp70 chaperones.

scientific article published in August 2004

Mechanisms of Cotranslational Maturation of Newly Synthesized Proteins

scientific article published on 03 December 2018

Metazoan Hsp70 machines use Hsp110 to power protein disaggregation

scientific article

Metazoan Hsp70-based protein disaggregases: emergence and mechanisms

scientific article

Mitochondria tether protein trash to rejuvenate cellular environments

scientific article published on 01 October 2014

Modulation of Amyloid States by Molecular Chaperones

scientific article published on 01 July 2019

Modulation of substrate specificity of the DnaK chaperone by alteration of a hydrophobic arch

scientific article published on 01 December 2000

Molecular basis for interactions of the DnaK chaperone with substrates.

scientific article

Molecular basis for regulation of the heat shock transcription factor sigma32 by the DnaK and DnaJ chaperones.

scientific article

Molecular chaperones and protein quality control

scientific article (publication date: 5 May 2006)

Molecular chaperones: structure of a protein disaggregase

scientific article published on January 2004

Molecular dissection of amyloid disaggregation by human HSP70

scientific article published on 11 November 2020

Molecular mechanism and structure of Trigger Factor bound to the translating ribosome

scientific article

Molecular mechanism of Hsp70 chaperones

scientific article published in July 2002

Monitoring protein misfolding by site-specific labeling of proteins in vivo

scientific article

Multistep mechanism of substrate binding determines chaperone activity of Hsp70

scientific article published on 01 July 2000

Mutant Analysis Reveals Allosteric Regulation of ClpB Disaggregase ⬇️

scientific article published on 22 February 2017

Mutations in the DnaK chaperone affecting interaction with the DnaJ cochaperone.

scientific article

Novel insights into the mechanism of chaperone-assisted protein disaggregation

scientific article

Nucleotide Exchange Factors for Hsp70 Chaperones.

scientific article published in January 2018

Nucleotide exchange factors for Hsp70 chaperones

scientific article published on 01 January 2011

Nucleotide-induced conformational changes in the ATPase and substrate binding domains of the DnaK chaperone provide evidence for interdomain communication

scientific article published on 01 July 1995

Operon structure and cotranslational subunit association direct protein assembly in bacteria.

scientific article published on 24 September 2015

Pathways of allosteric regulation in Hsp70 chaperones.

scientific article

Physical Origins of Codon Positions That Strongly Influence Cotranslational Folding: A Framework for Controlling Nascent-Protein Folding

scientific article published on 30 December 2015

Physical interaction between heat shock proteins DnaK, DnaJ, and GrpE and the bacterial heat shock transcription factor sigma 32

scientific article published on May 29, 1992

Poly-L-lysine enhances the protein disaggregation activity of ClpB.

scientific article published in October 2003

Prevention and reversion of protein aggregation by molecular chaperones in the E. coli cytosol: implications for their applicability in biotechnology

scientific article

Principles of general and regulatory proteolysis by AAA+ proteases in Escherichia coli

scientific article published on 22 September 2009

Profiling Ssb-Nascent Chain Interactions Reveals Principles of Hsp70-Assisted Folding

scientific article published in July 2017

Prolonged starvation drives reversible sequestration of lipid biosynthetic enzymes and organelle reorganization in Saccharomyces cerevisiae

scientific article published on 11 March 2015

Protein Disaggregation in Multicellular Organisms

scientific article published on 28 February 2018

Protein disaggregation by the AAA+ chaperone ClpB involves partial threading of looped polypeptide segments

scientific article

Protein folding and degradation in bacteria: to degrade or not to degrade? That is the question.

scientific article

Protein quality control in the cytosol and the endoplasmic reticulum: brothers in arms.

scientific article published on October 2010

Protein turnover: a CHIP programmed for proteolysis.

scientific article published in January 2002

Proteolysis in prokaryotes: protein quality control and regulatory principles

scientific article published in September 2003

Proteolysis of the phage lambda CII regulatory protein by FtsH (HflB) of Escherichia coli.

scientific article

Quantitative and spatio-temporal features of protein aggregation in Escherichia coli and consequences on protein quality control and cellular ageing

scientific article

Reconstitution of maltose transport in Escherichia coli: conditions affecting import of maltose-binding protein into the periplasm of calcium-treated cells.

scientific article

Refolding of substrates bound to small Hsps relies on a disaggregation reaction mediated most efficiently by ClpB/DnaK.

scientific article

Regulation of the Escherichia coli heat-shock response.

scientific article

Regulatory coiled-coil domains promote head-to-head assemblies of AAA+ chaperones essential for tunable activity control

scientific article published on 22 November 2017

Regulatory region C of the E. coli heat shock transcription factor, sigma32, constitutes a DnaK binding site and is conserved among eubacteria.

scientific article published on March 1996

Reshaping of the conformational search of a protein by the chaperone trigger factor.

scientific article published on 7 July 2013

Ribosomes catch Hsp70s

scientific article

Role for ribosome-associated complex and stress-seventy subfamily B (RAC-Ssb) in integral membrane protein translation.

scientific article published on 2 October 2017

Role of region C in regulation of the heat shock gene-specific sigma factor of Escherichia coli, sigma32

scientific article

Role of sHsps in organizing cytosolic protein aggregation and disaggregation

scientific article published on 24 January 2017

Role of the DnaK and HscA homologs of Hsp70 chaperones in protein folding in E.coli ⬇️

scientific article published on August 17, 1998

Roles of individual domains and conserved motifs of the AAA+ chaperone ClpB in oligomerization, ATP hydrolysis, and chaperone activity

scientific article published on 6 March 2003

SecA Cotranslationally Interacts with Nascent Substrate Proteins In Vivo

scientific article

SecA interacts with ribosomes in order to facilitate posttranslational translocation in bacteria

scientific article published in February 2011

Selective ribosome profiling as a tool for studying the interaction of chaperones and targeting factors with nascent polypeptide chains and ribosomes

scientific article

Selective ribosome profiling reveals the cotranslational chaperone action of trigger factor in vivo

scientific article

Signature of protein unfolding in chemical exchange saturation transfer imaging

scientific article

Size-dependent disaggregation of stable protein aggregates by the DnaK chaperone machinery.

scientific article published in July 2000

Small heat shock proteins sequester misfolding proteins in near-native conformation for cellular protection and efficient refolding.

scientific article

Small heat shock proteins, ClpB and the DnaK system form a functional triade in reversing protein aggregation

scientific article published on 01 October 2003

Solubilization of aggregated proteins by ClpB/DnaK relies on the continuous extraction of unfolded polypeptides

scientific article published in December 2004

Spatially organized aggregation of misfolded proteins as cellular stress defense strategy

scientific article

Stand-alone ClpG disaggregase confers superior heat tolerance to bacteria

scientific article published on 20 December 2017

Structural analysis of a signal peptide inside the ribosome tunnel by DNP MAS NMR

scientific article

Structural analysis of the adaptor protein ClpS in complex with the N-terminal domain of ClpA

scientific article

Structural analysis of the ribosome-associated complex (RAC) reveals an unusual Hsp70/Hsp40 interaction

scientific article

Structural pathway of regulated substrate transfer and threading through an Hsp100 disaggregase.

scientific article

Structure and function of a novel type of ATP-dependent Clp protease

scientific article published on 23 February 2009

Structure and function of the molecular chaperone Trigger Factor.

scientific article

Structure-function analysis of HscC, the Escherichia coli member of a novel subfamily of specialized Hsp70 chaperones

scientific article

Substrate binding by the yeast Hsp110 nucleotide exchange factor and molecular chaperone Sse1 is not obligate for its biological activities

scientific article published on 24 May 2017

Substrate recognition by the AAA+ chaperone ClpB.

scientific article

Substrate shuttling between the DnaK and GroEL systems indicates a chaperone network promoting protein folding.

scientific article

Substrate threading through the central pore of the Hsp104 chaperone as a common mechanism for protein disaggregation and prion propagation

scientific article published on 28 February 2008

Systemic control of protein synthesis through sequestration of translation and ribosome biogenesis factors during severe heat stress

scientific article published on 17 October 2015

Targeted Delivery of an ssrA-Tagged Substrate by the Adaptor Protein SspB to Its Cognate AAA+ Protein ClpX ⬇️

scientific article published on August 1, 2003

Telling right from wrong in life - cellular quality control

scientific article

The Amino-terminal 118 Amino Acids of Escherichia coli Trigger Factor Constitute a Domain That Is Necessary and Sufficient for Binding to Ribosomes ⬇️

scientific article published on August 29, 1997

The C terminus of sigma(32) is not essential for degradation by FtsH.

scientific article published on October 2001

The C-terminal domain of Escherichia coli trigger factor represents the central module of its chaperone activity

scientific article

The C-terminal tail of the bacterial translocation ATPase SecA modulates its activity

scientific article published on 27 June 2019

The DnaK chaperone system of Escherichia coli: quaternary structures and interactions of the DnaK and GrpE components.

scientific article

The Hsp40 J-domain modulates Hsp70 conformation and ATPase activity with a semi-elliptical spring

scientific article published on 7 July 2017

The Hsp70 and Hsp60 Chaperone Machines ⬇️

scientific article (publication date: 6 February 1998)

The Hsp70 chaperone network

scientific article published on 01 November 2019

The N-end rule pathway for regulated proteolysis: prokaryotic and eukaryotic strategies

scientific article published on 15 February 2007

The Rqc2/Tae2 subunit of the ribosome-associated quality control (RQC) complex marks ribosome-stalled nascent polypeptide chains for aggregation

scientific article

The endoplasmic reticulum Grp170 acts as a nucleotide exchange factor of Hsp70 via a mechanism similar to that of the cytosolic Hsp110.

scientific article

The growing world of small heat shock proteins: from structure to functions

scientific article published on 31 March 2017

The heat shock response of Escherichia coli.

scientific article

The human DnaJ homologue dj2 facilitates mitochondrial protein import and luciferase refolding

scientific article published on December 1997

The ribosome as a platform for co-translational processing, folding and targeting of newly synthesized proteins

scientific article published on June 2009

The role of ATP in the functional cycle of the DnaK chaperone system

scientific article published on 01 May 1995

The second step of ATP binding to DnaK induces peptide release.

scientific article

The yeast AAA+ chaperone Hsp104 is part of a network that links the actin cytoskeleton with the inheritance of damaged proteins

scientific article published on 27 April 2009

Thermotolerance requires refolding of aggregated proteins by substrate translocation through the central pore of ClpB.

scientific article

Three-state equilibrium of Escherichia coli trigger factor

scientific article

Toc12, a novel subunit of the intermembrane space preprotein translocon of chloroplasts

scientific article published on 18 August 2004

Towards a unifying mechanism for ClpB/Hsp104-mediated protein disaggregation and prion propagation

scientific article published on February 2010

Toxic Activation of an AAA+ Protease by the Antibacterial Drug Cyclomarin A

scientific article published on 13 June 2019

Translation suppression promotes stress granule formation and cell survival in response to cold shock

scientific article

Trigger Factor Reduces the Force Exerted on the Nascent Chain by a Cotranslationally Folding Protein

scientific article published on 18 February 2016

Trigger Factor and DnaK possess overlapping substrate pools and binding specificities

scientific article

Trigger factor binds to ribosome-signal-recognition particle (SRP) complexes and is excluded by binding of the SRP receptor

scientific article

Trigger factor finds new jobs and contacts

scientific article

Trigger factor forms a protective shield for nascent polypeptides at the ribosome

scientific article

Trigger factor in complex with the ribosome forms a molecular cradle for nascent proteins

scientific article

Trigger factor peptidyl-prolyl cis/trans isomerase activity is not essential for the folding of cytosolic proteins in Escherichia coli

scientific article

Tuning of chaperone activity of Hsp70 proteins by modulation of nucleotide exchange.

scientific article published in May 2001

Two-Step Activation Mechanism of the ClpB Disaggregase for Sequential Substrate Threading by the Main ATPase Motor

scientific article published on 01 June 2019

Unscrambling an egg: protein disaggregation by AAA+ proteins

scientific article

YfhJ, a Molecular Adaptor in Iron-Sulfur Cluster Formation or a Frataxin-like Protein?

scientific article published on 01 May 2006

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