List of works by Holger Stark

3D cryo-EM structure of an active step I spliceosome and localization of its catalytic core

scientific article

APC15 mediates CDC20 autoubiquitylation by APC/C(MCC) and disassembly of the mitotic checkpoint complex.

scientific article published on 24 September 2012

Aerosolized nanogram quantities of plasmid DNA mediate highly efficient gene delivery to mouse airway epithelium

scientific article published in September 2005

An approach for de novo structure determination of dynamic molecular assemblies by electron cryomicroscopy

scientific article published in June 2010

An assembly chaperone collaborates with the SMN complex to generate spliceosomal SnRNPs

scientific article

Atomic-resolution protein structure determination by cryo-EM

scientific article published on 21 October 2020

Automated correlation of single particle tilt pairs for Random Conical Tilt and Orthogonal Tilt Reconstructions

scientific article published on 7 November 2012

Characterization of purified human Bact spliceosomal complexes reveals compositional and morphological changes during spliceosome activation and first step catalysis

scientific article

Characterization of tailor-made copolymers of oligo(ethylene glycol) methyl ether methacrylate and N,N-dimethylaminoethyl methacrylate as nonviral gene transfer agents: influence of macromolecular structure on gene vector particle properties and tra

scientific article published in January 2010

Chromatin affinity purification and quantitative mass spectrometry defining the interactome of histone modification patterns.

scientific article

Composition and three-dimensional EM structure of double affinity-purified, human prespliceosomal A complexes

scientific article

Conformational rearrangements upon start codon recognition in human 48S translation initiation complex

scientific article published on 30 April 2022

Conformational rearrangements upon start codon recognition in human 48S translation initiation complex

preprint article published on 20 December 2021

Correction: Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation

scientific article published on 16 March 2016

Corrim-based alignment for improved speed in single-particle image processing

scientific article published on 01 September 2003

Cryo-EM analyses of dimerized spliceosomes provide new insights into the functions of B complex proteins

Cryo-EM in drug discovery: achievements, limitations and prospects

article

Cryo-electron microscopy of spliceosomal components

scientific article

Crystal structure of a core spliceosomal protein interface

scholarly article

Discovery of a Regulatory Subunit of the Yeast Fatty Acid Synthase

scientific article published on 10 March 2020

Dual RING E3 Architectures Regulate Multiubiquitination and Ubiquitin Chain Elongation by APC/C.

scientific article

Electron microscopy structure of human APC/CCDH1–EMI1 reveals multimodal mechanism of E3 ligase shutdown

scientific article

Electron radiation damage to protein crystals of bacteriorhodopsin at different temperatures

scientific article published in June 1996

Energy barriers and driving forces in tRNA translocation through the ribosome

scientific article

Exon, intron and splice site locations in the spliceosomal B complex

scientific article published on 18 June 2009

GraDeR: Membrane Protein Complex Preparation for Single-Particle Cryo-EM.

scientific article published on 10 August 2015

GraFix: sample preparation for single-particle electron cryomicroscopy

scientific article

Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy

scientific article

Localization of the coactivator Cdh1 and the cullin subunit Apc2 in a cryo-electron microscopy model of vertebrate APC/C

scientific article

Major Conformational Change in the Complex SF3b upon Integration into the Spliceosomal U11/U12 di-snRNP as Revealed by Electron Cryomicroscopy

scientific article (publication date: March 2005)

Mechanism of APC/CCDC20 activation by mitotic phosphorylation

scientific article published on 25 April 2016

Mechanism of polyubiquitination by human anaphase-promoting complex: RING repurposing for ubiquitin chain assembly.

scientific article published on 9 October 2014

Mechanism of protein-guided folding of the active site U2/U6 RNA during spliceosome activation

scientific article published on 26 November 2020

Merging molecular electron microscopy and mass spectrometry by carbon film-assisted endoproteinase digestion

scientific article published on 8 June 2010

Molecular Architecture of the Multiprotein Splicing Factor SF3b ⬇️

scientific article (publication date: 9 May 2003)

Molecular Basis of Transcription-Coupled Pre-mRNA Capping

scientific article published on 7 May 2015

Molecular architecture of the Saccharomyces cerevisiae activated spliceosome

scientific article

Molecular architecture of the human 17S U2 snRNP

scientific article published on 03 June 2020

Molecular architecture of the human U4/U6.U5 tri-snRNP

scientific article

Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation.

scientific article published on 3 December 2015

Novel insights into the architecture and protein interaction network of yeast eIF3

scientific article

Organization of core spliceosomal components U5 snRNA loop I and U4/U6 Di-snRNP within U4/U6.U5 Tri-snRNP as revealed by electron cryomicroscopy

scientific article

Posing the APC/C E3 Ubiquitin Ligase to Orchestrate Cell Division

scientific article published on 25 October 2018

Protein composition and electron microscopy structure of affinity-purified human spliceosomal B complexes isolated under physiological conditions

scientific article published on July 2006

Protein engineering of a ubiquitin-variant inhibitor of APC/C identifies a cryptic K48 ubiquitin chain binding site

scientific article published on 26 July 2019

ProteoPlex: stability optimization of macromolecular complexes by sparse-matrix screening of chemical space

scientific article (publication date: September 2015)

RING E3 mechanism for ubiquitin ligation to a disordered substrate visualized for human anaphase-promoting complex

scientific article published on 30 March 2015

Reconstitution of both steps of Saccharomyces cerevisiae splicing with purified spliceosomal components

scientific article

Ribosome dynamics and tRNA movement by time-resolved electron cryomicroscopy

scientific article

Ribosome interactions of aminoacyl-tRNA and elongation factor Tu in the codon-recognition complex

scientific article

Self-assembly of a nanoscale DNA box with a controllable lid

scientific article

Self-assembly of ternary insulin-polyethylenimine (PEI)-DNA nanoparticles for enhanced gene delivery and expression in alveolar epithelial cells

scientific article

Snapshots of the RNA editing machine in trypanosomes captured at different assembly stages in vivo

scientific article published on 5 February 2009

Spontaneous reverse movement of mRNA-bound tRNA through the ribosome.

scientific article published on 18 March 2007

Structural Insights into Nuclear pre-mRNA Splicing in Higher Eukaryotes

scientific article published on 01 November 2019

Structural Insights into the Roles of Metazoan-Specific Splicing Factors in the Human Step 1 Spliceosome

scientific article published on 01 October 2020

Structural basis for cooperativity of CRM1 export complex formation

scientific article

Structural basis for the function of the ribosomal L7/12 stalk in factor binding and GTPase activation

scientific article published on July 2005

Structural basis of assembly chaperone- mediated snRNP formation

scientific article

Structural determinants and mechanism of mammalian CRM1 allostery

scientific article published on 11 July 2013

Structural insight into filament formation by mammalian septins

scientific article

Structural insights into how Prp5 proofreads the pre-mRNA branch site

scientific article published in August 2021

Structural insights into the cross-exon to cross-intron spliceosome switch

scientific article published in June 2024

Structural mapping of spliceosomes by electron microscopy

scientific article published on 09 February 2009

Structure of the E. coli ribosome-EF-Tu complex at <3 Å resolution by Cs-corrected cryo-EM

scientific article

Structure of the Lassa virus nucleoprotein revealed by X-ray crystallography, small-angle X-ray scattering, and electron microscopy

scientific article

Structure of the anaphase-promoting complex/cyclosome interacting with a mitotic checkpoint complex

scientific article

Structure of the hepatitis C virus IRES bound to the human 80S ribosome: remodeling of the HCV IRES.

scientific article published in November 2005

Structure of yeast U6 snRNPs: arrangement of Prp24p and the LSm complex as revealed by electron microscopy

scientific article

Substrate binding on the APC/C occurs between the coactivator Cdh1 and the processivity factor Doc1.

scientific article published on 26 December 2010

Systematic analysis of human protein complexes identifies chromosome segregation proteins

scientific article

The evolutionarily conserved core design of the catalytic activation step of the yeast spliceosome

scientific article

Three-dimensional reconstruction of Trypanosoma brucei editosomes using single-particle electron microscopy

scientific article published on January 2011

Three-dimensional structure of a pre-catalytic human spliceosomal complex B.

scientific article published on 18 April 2004

Towards understanding selenocysteine incorporation into bacterial proteins

scientific article published on October 2007

biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes

scientific article published on 25 April 2016

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