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List of works by Jochen Büchs

"Enzyme Test Bench," a high-throughput enzyme characterization technique including the long-term stability

scientific article published in June 2009

A New Automatic System to Evaluate the Density of Viable Microorganisms in Liquids

article by Jochen Büchs et al published September 1985 in Systematic and Applied Microbiology

A Synthetic Reaction Cascade Implemented by Colocalization of Two Proteins within Catalytically Active Inclusion Bodies

scientific article published on 20 August 2018

A novel parallel shaken bioreactor system for continuous operation

scientific article published in November 2004

A particular silent codon exchange in a recombinant gene greatly influences host cell metabolic activity

scientific article

A simple and inexpensive method for investigating microbiological, enzymatic, or inorganic catalysis using standard histology and microbiology laboratory equipment: assembly, mass transfer properties, hydrodynamic conditions and evaluation.

scientific article published in July 2006

Acetate-glycerol cometabolism: cultivating Schizosaccharomyces pombe on a non-fermentable carbon source in a defined minimal medium

scientific article published on 30 March 2011

Advances in shaking technologies

scientific article published on 18 April 2012

Alginate synthesis in Azotobacter vinelandii is increased by reducing the intracellular production of ubiquinone

scientific article published on 10 August 2012

An activity, stability and selectivity comparison of propioin synthesis by thiamine diphosphate-dependent enzymes in a solid/gas bioreactor

scientific article published in June 2007

An experimental comparison of respiration measuring techniques in fermenters and shake flasks: exhaust gas analyzer vs. RAMOS device vs. respirometer

scientific article published on 26 September 2006

An integrated catalytic approach to fermentable sugars from cellulose

scientific article published on October 2010

Anionic Extraction for Efficient Recovery of Biobased 2,3-Butanediol-A Platform for Bulk and Fine Chemicals.

scientific article published on 26 June 2017

Application of an improved continuous parallel shaken bioreactor system for three microbial model systems

scientific article published on 4 January 2008

Assaying CO2 release for determination of formate dehydrogenase activity in entrapment matrices and aqueous-organic two-phase systems

scientific article published in September 2006

Asymmetric division of Hansenula polymorpha reflected by a drop of light scatter intensity measured in batch microtiter plate cultivations at phosphate limitation.

scientific article

Biocatalytic conversion of lignocellulose to platform chemicals

scientific article published on 25 July 2012

Biomass pretreatment affects Ustilago maydis in producing itaconic acid

scientific article (publication date: 5 April 2012)

Bioprocess control in microscale: scalable fermentations in disposable and user-friendly microfluidic systems

scientific article

Branched chain amino acids maintain the molecular weight of poly(γ-glutamic acid) of Bacillus licheniformis ATCC 9945 during the fermentation

scientific article published on 18 May 2016

Cellulolytic RoboLector - towards an automated high-throughput screening platform for recombinant cellulase expression

scientific article

Characterisation of operation conditions and online monitoring of physiological culture parameters in shaken 24-well microtiter plates.

scientific article published on 5 November 2005

Characterisation of the gas-liquid mass transfer in shaking bioreactors

scientific article published on 01 March 2001

Characterization of Endogenous and Reduced Promoters for Oxygen-Limited Processes Using Escherichia coli.

scientific article published on 7 October 2016

Characterization of antibacterial polyethersulfone membranes using the Respiration Activity Monitoring System (RAMOS).

scientific article published on 20 July 2012

Characterization of hydromechanical stress in aerated stirred tanks up to 40 m(3) scale by measurement of maximum stable drop size

scientific article published on 7 July 2014

Combination of On-line pH and Oxygen Transfer Rate Measurement in Shake Flasks by Fiber Optical Technique and Respiration Activity MOnitoring System (RAMOS).

scientific article published on 20 December 2007

Combined dissolved oxygen tension and online viscosity measurements in shake flask cultivations via infrared fluorescent oxygen-sensitive nanoparticles

scientific article published on 10 September 2019

Comparison of two methods for designing calorimeters using stirred tank reactors.

scientific article published on 25 July 2012

Comprehensive characterization of two different Nicotiana tabacum cell lines leads to doubled GFP and HA protein production by media optimization

scientific article

Contact-free determination of viscosity in multiple parallel samples.

scientific article published on 6 June 2019

Continuous production and recovery of itaconic acid in a membrane bioreactor

scientific article published on 16 March 2013

Controlling pH in shake flasks using polymer-based controlled-release discs with pre-determined release kinetics

scientific article

Correlation between mass transfer coefficient kLa and relevant operating parameters in cylindrical disposable shaken bioreactors on a bench-to-pilot scale.

scientific article published on 2 December 2013

Cross-section perimeter is a suitable parameter to describe the effects of different baffle geometries in shaken microtiter plates

scientific article

Definition of culture conditions for Arxula adeninivorans, a rational basis for studying heterologous gene expression in this dimorphic yeast.

scientific article published on 25 March 2014

Determination of CO₂ sensitivity of micro-organisms in shaken bioreactors. I. Novel method based on the resistance of sterile closure

scientific article published on December 2010

Determination of CO₂ sensitivity of micro-organisms in shaken bioreactors. II. Novel online monitoring method

scientific article published on 01 December 2010

Development of a modified Respiration Activity Monitoring System for accurate and highly resolved measurement of respiration activity in shake flask fermentations

scientific article published on 17 August 2012

Development of an unsteady-state model for a biological system in miniaturized bioreactors

scientific article published on 13 October 2009

Device for sterile online measurement of the oxygen transfer rate in shaking flasks

scientific article published on 01 March 2001

Easy to use and reliable technique for online dissolved oxygen tension measurement in shake flasks using infrared fluorescent oxygen-sensitive nanoparticles.

scientific article

Editorial: ECAB focus issue: Engineered catalysts, robust, cost-effective and integrated bioprocesses and high-throughput screening

scientific article published in December 2016

Effect of additives on gas-phase catalysis with immobilised Thermoanaerobacter species alcohol dehydrogenase (ADH T).

scientific article

Effect of elevated dissolved carbon dioxide concentrations on growth of Corynebacterium glutamicum on D-glucose and L-lactate

scientific article published on 10 January 2007

Effect of oxygen limitation and medium composition on Escherichia coli fermentation in shake-flask cultures

scientific article

Effect of oxygen supply on passaging, stabilising and screening of recombinant Hansenula polymorpha production strains in test tube cultures

scientific article published in November 2003

Efficient evaluation of cellulose digestibility by Trichoderma reesei Rut-C30 cultures in online monitored shake flasks

scientific article

Electrocatalytic upgrading of itaconic acid to methylsuccinic acid using fermentation broth as a substrate solution

Elucidation of auxotrophic deficiencies of Bacillus pumilus DSM 18097 to develop a defined minimal medium.

scientific article published on 9 July 2018

Enzyme activity deviates due to spatial and temporal temperature profiles in commercial microtiter plate readers

scientific article published on 28 December 2015

Equalizing growth in high-throughput small scale cultivations via precultures operated in fed-batch mode

scientific article

Establishing a Fed-Batch Process for Protease Expression with Bacillus licheniformis in Polymer-Based Controlled-Release Microtiter Plates

scientific article published on 19 September 2019

Evaluation of microbial globin promoters for oxygen-limited processes using Escherichia coli.

scientific article published on 13 November 2017

Evidence for a key role of cytochrome bo3 oxidase in respiratory energy metabolism of Gluconobacter oxydans

scientific article published on 12 July 2013

Exchange of single amino acids at different positions of a recombinant protein affects metabolic burden in Escherichia coli

scientific article published on 23 January 2015

Extension and application of the "enzyme test bench" for oxygen consuming enzyme reactions

scientific article published on 11 September 2013

Fast automated online xylanase activity assay using HPAEC-PAD.

scientific article

Feasibility of gas/solid carboligation: conversion of benzaldehyde to benzoin using thiamine diphosphate-dependent enzymes.

scientific article published on 27 February 2007

Fed-batch mode in shake flasks by slow-release technique.

scientific article published in October 2006

Fed-batch operation in special microtiter plates: a new method for screening under production conditions.

scientific article

Flavin mononucleotide-based fluorescent reporter proteins outperform green fluorescent protein-like proteins as quantitative in vivo real-time reporters

scientific article

From beech wood to itaconic acid: case study on biorefinery process integration

scientific article published on 11 October 2018

Glucose oxidation by Gluconobacter oxydans: characterization in shaking-flasks, scale-up and optimization of the pH profile.

scientific article published on 26 February 2003

Glucose-containing polymer rings enable fed-batch operation in microtiter plates with parallel online measurement of scattered light, fluorescence, dissolved oxygen tension, and pH

scientific article published on 02 July 2019

High cell density cultivation of recombinant yeasts and bacteria under non-pressurized and pressurized conditions in stirred tank bioreactors

scientific article published on 23 June 2007

High cell-density processes in batch mode of a genetically engineered Escherichia coli strain with minimized overflow metabolism using a pressurized bioreactor.

scientific article published on 12 July 2010

High-throughput screening for ionic liquids dissolving (ligno-)cellulose

scientific article published on 20 January 2009

High-throughput screening of Hansenula polymorpha clones in the batch compared with the controlled-release fed-batch mode on a small scale

scientific article published on 9 October 2009

How recombinant swollenin from Kluyveromyces lactis affects cellulosic substrates and accelerates their hydrolysis

scientific article published on 23 September 2011

Hydromechanical stress in shake flasks: correlation for the maximum local energy dissipation rate

scientific article published in April 2006

Immobilization conditions of ketoreductase on enantioselective reduction in a gas-solid bioreactor

scientific article published in May 2010

Impact of two ionic liquids, 1-ethyl-3-methylimidazolium acetate and 1-ethyl-3-methylimidazolium methylphosphonate, on Saccharomyces cerevisiae: metabolic, physiologic, and morphological investigations

scientific article

Improvement and scale-down of a Trichoderma reesei shake flask protocol to microtiter plates enables high-throughput screening

scientific article published on 27 June 2014

In situ cell retention of a CHO culture by a reverse-flow diafiltration membrane bioreactor

scientific article published on 18 September 2014

In situ product recovery of single-chain antibodies in a membrane bioreactor.

scientific article published in August 2014

Increased product formation induced by a directed secondary substrate limitation in a batch Hansenula polymorpha culture.

scientific article published on 27 October 2009

Influence of nitrogen source and pH value on undesired poly(γ-glutamic acid) formation of a protease producing Bacillus licheniformis strain.

scientific article published on 8 July 2015

Introducing substrate limitations to overcome catabolite repression in a protease producing Bacillus licheniformis strain using membrane-based fed-batch shake flasks

scientific article published on 28 February 2019

Introduction to advantages and problems of shaken cultures

scientific article published on 01 March 2001

Investigation of poly(γ-glutamic acid) production via online determination of viscosity and oxygen transfer rate in shake flasks.

scientific article published on 12 July 2017

Investigation of vinegar production using a novel shaken repeated batch culture system

scientific article

Itaconic acid--a biotechnological process in change

scientific article published on 08 December 2012

Kluyveromyces marxianus, an Attractive Yeast for Ethanolic Fermentation in the Presence of Imidazolium Ionic Liquids.

scientific article

Light-controlled gene expression in yeast using photocaged Cu2.

scientific article published on 25 April 2017

Light-induced gene expression with photocaged IPTG for induction profiling in a high-throughput screening system

scientific article published on 23 April 2016

Linear correlation between online capacitance and offline biomass measurement up to high cell densities in Escherichia coli fermentations in a pilot-scale pressurized bioreactor

scientific article published in February 2011

Liquid films on shake flask walls explain increasing maximum oxygen transfer capacities with elevating viscosity

scientific article published on 29 August 2013

Mass transfer resistance of sterile plugs in shaking bioreactors

scientific article published on 01 March 2001

Mechanistic kinetic model for symmetric carboligations using benzaldehyde lyase

scientific article published in September 2008

Metabolic activity of Corynebacterium glutamicum grown on L: -lactic acid under stress

scientific article

Metabolome analysis reveals the effect of carbon catabolite control on the poly(γ-glutamic acid) biosynthesis of Bacillus licheniformis ATCC 9945.

scientific article published on 23 September 2015

Method for quantitative determination of spatial polymer distribution in alginate beads using Raman spectroscopy

scientific article published in March 2005

Microfluidic biolector-microfluidic bioprocess control in microtiter plates.

scientific article

Microscale and miniscale fermentation and screening

scientific article published on 23 December 2014

Minireactor-based high-throughput temperature profiling for the optimization of microbial and enzymatic processes

scientific article

Model discrimination for the propionic acid diffusion into hydrogel beads using lifetime confocal laser scanning microscopy

article

Modelling and advanced understanding of unsteady-state gas transfer in shaking bioreactors

scientific article published in January 2007

Necessity of a two-stage process for the production of azadirachtin-related limonoids in suspension cultures of Azadirachta indica

scientific article published in January 2003

New insights in the spatially resolved dynamic pH measurement in macroscopic large absorbent particles by confocal laser scanning microscopy.

scientific article

New method to determine the mass transfer resistance of sterile closures for shaken bioreactors

scientific article published in December 2007

Newly designed and validated impedance spectroscopy setup in microtiter plates successfully monitors viable biomass online

scientific article published on 26 May 2015

Non-invasive online detection of microbial lysine formation in stirred tank bioreactors by using calorespirometry

scientific article published on 17 January 2013

Noninvasive tool for optical online monitoring of individual biomass concentrations in a defined coculture

scientific article published on 13 January 2020

Novel plasmid-free Gluconobacter oxydans strains for production of the natural sweetener 5-ketofructose

scientific article published on 04 March 2020

Novel technique for high throughput measurement of active monooxygenase concentration

scientific article published on 14 November 2016

Online determination of viable biomass up to very high cell densities in Arxula adeninivorans fermentations using an impedance signal

scientific article

Online evaluation of the metabolic activity of Ustilago maydis on (poly)galacturonic acid

scientific article published on 18 December 2018

Online in vivo monitoring of cytosolic NAD redox dynamics in Ustilago maydis

scientific article published on 22 May 2018

Online measurement of the respiratory activity in shake flasks enables the identification of cultivation phases and patterns indicating recombinant protein production in various E. coli host strains.

scientific article published on 5 January 2018

Online measurement of viscosity for biological systems in stirred tank bioreactors.

scientific article published on 14 November 2016

Online monitoring of dissolved oxygen tension in microtiter plates based on infrared fluorescent oxygen-sensitive nanoparticles

scientific article

Online monitoring of fermentation processes via non-invasive low-field NMR.

scientific article

Online respiration activity measurement (OTR, CTR, RQ) in shake flasks

Optical method for the determination of the oxygen-transfer capacity of small bioreactors based on sulfite oxidation

scientific article published on 01 September 2001

Optimization of adsorptive immobilization of alcohol dehydrogenases

scientific article published in April 2005

Optimization of enzymatic gas-phase reactions by increasing the long-term stability of the catalyst

scientific article

Optimized polymer-based glucose release in microtiter plates for small-scale E. coli fed-batch cultivations

scientific article published on 27 August 2020

Optimizing recombinant protein expression via automated induction profiling in microtiter plates at different temperatures

scientific article published on 28 November 2017

Optogenetic Regulation of Tunable Gene Expression in Yeast Using Photo-Labile Caged Methionine.

scientific article published on 29 August 2016

Orbitally shaken single-use bioreactors

scientific article

Out-of-phase operating conditions, a hitherto unknown phenomenon in shaking bioreactors.

scientific article

Oxygen limitation is a pitfall during screening for industrial strains

scientific article published on 31 March 2006

Oxygen supply controls the onset of pristinamycins production by Streptomyces pristinaespiralis in shaking flasks

scientific article published on 02 May 2011

Oxygen transfer phenomena in 48-well microtiter plates: determination by optical monitoring of sulfite oxidation and verification by real-time measurement during microbial growth

scientific article published in March 2005

Oxygen transfer rate identifies priming compounds in parsley cells.

scientific article published on 25 November 2015

Parallel online determination of ethylene release rate by Shaken Parsley cell cultures using a modified RAMOS device.

scientific article published in June 2018

Parallel online multi-wavelength (2D) fluorescence spectroscopy in each well of a continuously shaken microtiter plate

scientific article published on 13 October 2016

Parallel substrate supply and pH stabilization for optimal screening of E. coli with the membrane-based fed-batch shake flask.

scientific article

Parallel use of shake flask and microtiter plate online measuring devices (RAMOS and BioLector) reduces the number of experiments in laboratory-scale stirred tank bioreactors.

scientific article published on 30 May 2015

Permeability of currently available microtiter plate sealing tapes fail to fulfil the requirements for aerobic microbial cultivation

scientific article published on 31 August 2016

Phenotyping the quality of complex medium components by simple online-monitored shake flask experiments

scientific article published on 7 November 2014

Photocaged Arabinose: A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression

scientific article published on 16 December 2015

Physiological relation between respiration activity and heterologous expression of selected benzoylformate decarboxylase variants in Escherichia coli

scientific article published on 19 October 2010

Pitfalls in optical on-line monitoring for high-throughput screening of microbial systems

scientific article

Polymer-based ammonium-limited fed-batch cultivation in shake flasks improves lipid productivity of the microalga Chlorella vulgaris

scientific article published on 16 July 2019

Polymer-based controlled-release fed-batch microtiter plate - diminishing the gap between early process development and production conditions

article

Potential errors in conventional DOT measurement techniques in shake flasks and verification using a rotating flexitube optical sensor ⬇️

scientific article

Power consumption in shaking flasks on rotary shaking machines: I. Power consumption measurement in unbaffled flasks at low liquid viscosity.

scientific article published in June 2000

Practical screening of purified cellobiohydrolases and endoglucanases with α-cellulose and specification of hydrodynamics

scientific article (publication date: 18 August 2010)

Precultures Grown under Fed-Batch Conditions Increase the Reliability and Reproducibility of High-Throughput Screening Results

scientific article published on 05 August 2019

Prediction of Escherichia coli expression performance in microtiter plates by analyzing only the temporal development of scattered light during culture

scientific article published on 3 July 2017

Prediction of recombinant protein production by Escherichia coli derived online from indicators of metabolic burden

scientific article published on 24 September 2018

Predictive tool for recombinant protein production in Escherichia coli shake-flask cultures using an on-line monitoring system.

scientific article

Probing unnatural amino acid integration into enhanced green fluorescent protein by genetic code expansion with a high-throughput screening platform

scientific article published on 30 September 2016

Process development in Hansenula polymorpha and Arxula adeninivorans, a re-assessment

scientific article published on 15 April 2009

Process relevant screening of cellulolytic organisms for consolidated bioprocessing.

scientific article published on 24 April 2017

Production of the potential sweetener 5-ketofructose from fructose in fed-batch cultivation with Gluconobacter oxydans

scientific article published on 9 March 2018

Production of uroporphyrinogen III, which is the common precursor of all tetrapyrrole cofactors, from 5-aminolevulinic acid by Escherichia coli expressing thermostable enzymes

scientific article published on 21 April 2013

Promoters from the itaconate cluster of Ustilago maydis are induced by nitrogen depletion.

scientific article published on 28 November 2017

Propioin synthesis using thiamine diphosphate-dependent enzymes

scientific article published in January 2009

Quantifying the sensitivity of G. oxydans ATCC 621H and DSM 3504 to osmotic stress triggered by soluble buffers.

scientific article published on 3 February 2015

Quasi-continuous combined scattered light and fluorescence measurements: a novel measurement technique for shaken microtiter plates.

scientific article published in October 2005

Quasi-continuous parallel online scattered light, fluorescence and dissolved oxygen tension measurement combined with monitoring of the oxygen transfer rate in each well of a shaken microtiter plate.

scientific article published on 3 December 2016

Rapid assessment of oxygen transfer impact for Corynebacterium glutamicum

scientific article published in July 2014

Real-time determination of intracellular oxygen in bacteria using a genetically encoded FRET-based biosensor

scientific article

Reassessing the out-of-phase phenomenon in shake flasks by evaluating the angle-dependent liquid distribution relative to the direction of the centrifugal acceleration

scientific article published on 06 September 2019

Replication methods and tools in high-throughput cultivation processes - recognizing potential variations of growth and product formation by on-line monitoring

scientific article

Respiration activity monitoring system (RAMOS), an efficient tool to study the influence of the oxygen transfer rate on the synthesis of lipopeptide by Bacillus subtilis ATCC6633.

scientific article published on 17 January 2008

Respiration activity monitoring system for any individual well of a 48-well microtiter plate.

scientific article

Robo-Lector - a novel platform for automated high-throughput cultivations in microtiter plates with high information content

scientific article published on August 2009

Scale-down of vinegar production into microtiter plates using a custom-made lid.

scientific article

Scale-up from microtiter plate to laboratory fermenter: evaluation by online monitoring techniques of growth and protein expression in Escherichia coli and Hansenula polymorpha fermentations

scientific article

Scale-up from shake flasks to fermenters in batch and continuous mode with Corynebacterium glutamicum on lactic acid based on oxygen transfer and pH.

scientific article published in November 2007

Scale-up of a Type I secretion system in E. coli using a defined mineral medium

scientific article published on 04 November 2019

Scaled-up manufacturing of recombinant antibodies produced by plant cells in a 200-L orbitally-shaken disposable bioreactor

scientific article published on 13 October 2014

Screening for enzyme activity in turbid suspensions with scattered light

scientific article published on 07 February 2011

Screening for optimal protease producing Bacillus licheniformis strains with polymer-based controlled-release fed-batch microtiter plates

scientific article published in 2021

Screening of cellulases for biofuel production: online monitoring of the enzymatic hydrolysis of insoluble cellulose using high-throughput scattered light detection

scientific article published on 01 January 2011

Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2,3-butanediol production

scientific article published on 03 May 2019

Short-chain fluorescent tryptophan tags for on-line detection of functional recombinant proteins

scientific article

Study on mesophilic and thermophilic alcohol dehydrogenases in gas-phase reaction

scientific article published in March 2006

Substrate and water adsorption phenomena in a gas/solid enzymatic reactor.

scientific article published in May 2009

Systematic determination of intrinsic reaction parameters in enzyme immobilizates

Systematic evaluation of characteristics of the membrane-based fed-batch shake flask.

scientific article published on 17 July 2017

Tackling destructive proteolysis of unconventionally secreted heterologous proteins in Ustilago maydis

scientific article published on 29 July 2018

Testing plasmid stability of Escherichia coli using the Continuously Operated Shaken BIOreactor System.

scientific article published on 4 September 2016

The baffled microtiter plate: increased oxygen transfer and improved online monitoring in small scale fermentations

scientific article published on 01 August 2009

The consequence of an additional NADH dehydrogenase paralog on the growth of Gluconobacter oxydans DSM3504.

scientific article

The metabolic switch can be activated in a recombinant strain of Streptomyces lividans by a low oxygen transfer rate in shake flasks ⬇️

scientific article published in 2018

The oxygen mass transfer, carbon dioxide inhibition, heat removal, and the energy and cost efficiencies of high pressure fermentation.

scientific article published in January 2005

The oxygen transfer rate as key parameter for the characterization of Hansenula polymorpha screening cultures

scientific article published on 28 October 2003

Three-dimensional (3D) evaluation of liquid distribution in shake flask using an optical fluorescence technique.

scientific article published on 03 August 2017

Time efficient way to calculate oxygen transfer areas and power input in cylindrical disposable shaken bioreactors

scientific article published on 5 September 2014

Time-Resolved Monitoring of the Oxygen Transfer Rate of Chinese Hamster Ovary Cells Provides Insights Into Culture Behavior in Shake Flasks

scientific article published on 25 August 2021

Transponder-based sensor for monitoring electrical properties of biological cell solutions

scientific article published in August 2005

Utilizing high-throughput experimentation to enhance specific productivity of an E.coli T7 expression system by phosphate limitation.

scientific article

Validation of a high-throughput fermentation system based on online monitoring of biomass and fluorescence in continuously shaken microtiter plates

scientific article published on 4 June 2009

Validation of the transferability of membrane-based fed-batch shake flask cultivations to stirred-tank reactor using three different protease producing Bacillus strains

scientific article published on 20 June 2019

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