List of works - Francisco J. Blanco

1H and 15N NMR assignment and solution structure of the SH3 domain of spectrin: comparison of unrefined and refined structure sets with the crystal structure

scientific article published on June 1, 1997

2D-NMR reveals different populations of exposed lysine residues in the apoB-100 protein of electronegative and electropositive fractions of LDL particles

scientific article published on 28 January 2010

A generalized approach for NMR studies of lipid–protein interactions based on sparse fluorination of acyl chains

scholarly article by Alfredo De Biasio et al published 2018 in Chemical Communications

A tumor-targeted trimeric 4-1BB-agonistic antibody induces potent anti-tumor immunity without systemic toxicity

scientific article published in Nature Communications

ATTACK, a novel bispecific T cell-recruiting antibody with trivalent EGFR binding and monovalent CD3 binding for cancer immunotherapy

scientific article published on 27 September 2017

Aggregated electronegative low density lipoprotein in human plasma shows a high tendency toward phospholipolysis and particle fusion

scientific article published on 29 July 2010

Backbone assignment of human proliferating cell nuclear antigen.

scientific article published on 30 November 2007

Backbone assignment of the 98 kDa homotrimeric yeast PCNA ring

scientific article published on 12 October 2006

Bacterial secretion of soluble and functional trivalent scFv-based N-terminal trimerbodies.

scientific article published on 4 August 2015

Binding site plasticity in viral PPxY Late domain recognition by the third WW domain of human NEDD4

scientific article published on 21 October 2019

Carcinoembryonic Antigen (CEA)-Specific 4-1BB-Costimulation Induced by CEA-Targeted 4-1BB-Agonistic Trimerbodies

scientific article published on 31 July 2019

Crystal Structure of Inhibitor of Growth 4 (ING4) Dimerization Domain Reveals Functional Organization of ING Family of Chromatin-binding Proteins

scientific article

Crystal Structure of the Homing Endonuclease I-CvuI Provides a New Template for Genome Modification.

scientific article

Crystal structure of I-DmoI in complex with its target DNA provides new insights into meganuclease engineering

scientific article

Crystal structure of the third extracellular domain of CD5 reveals the fold of a group B scavenger cysteine-rich receptor domain

scientific article

Crystallization and preliminary X-ray diffraction analysis of the dimerization domain of the tumour suppressor ING4.

scientific article

Crystallization and preliminary X-ray diffraction analysis of the homing endonuclease I-CvuI from Chlorella vulgaris in complex with its target DNA.

scientific article published on 22 January 2014

Crystallization and preliminary X-ray diffraction analysis on the homing endonuclease I-Dmo-I in complex with its target DNA.

scientific article published on 21 November 2007

Design, synthesis, and functional evaluation of leukocyte function associated antigen-1 antagonists in early and late stages of cancer development.

scientific article published on 22 January 2013

Disentangling polydispersity in the PCNA-p15PAF complex, a disordered, transient and multivalent macromolecular assembly.

scientific article

Double Monoubiquitination Modifies the Molecular Recognition Properties of p15PAF Promoting Binding to the Reader Module of Dnmt1

scientific article published on 18 September 2019

Efficient production of single-chain fragment variable-based N-terminal trimerbodies in Pichia pastoris

scientific article published on 12 August 2014

Efficient targeting of a SCID gene by an engineered single-chain homing endonuclease

scientific article

Engineering of large numbers of highly specific homing endonucleases that induce recombination on novel DNA targets

scientific article

Enhanced antiangiogenic therapy with antibody-collagen XVIII NC1 domain fusion proteins engineered to exploit matrix remodeling events.

scientific article published on July 2006

Evolution of SUMO Function and Chain Formation in Insects.

scientific article published on 4 November 2015

Expression, purification and crystallization of human CD5 domain III, a nano-scale crystallization example.

scientific article

Folding and aggregation kinetics of a beta-hairpin

scientific article

Folding of Protein G B1 Domain Studied by the Conformational Characterization of Fragments Comprising Its Secondary Structure Elements ⬇️

scientific article published on June 1, 1995

Generation and characterization of monospecific and bispecific hexavalent trimerbodies

scientific article published on 5 December 2012

Homing endonucleases: from basics to therapeutic applications.

scientific article published on March 2010

ICAM-1 peptide inhibitors of T-cell adhesion bind to the allosteric site of LFA-1. An NMR characterization

scientific article published on 14 September 2007

Impaired Function of Solute Carrier Family 19 Leads to Low Folate Levels and Lipid Droplet Accumulation in Hepatocytes

Improved stability of multivalent antibodies containing the human collagen XV trimerization domain.

scientific article published on March 2012

In vivo tumor targeting and imaging with engineered trivalent antibody fragments containing collagen-derived sequences

scientific article

Intramolecular trimerization, a novel strategy for making multispecific antibodies with controlled orientation of the antigen binding domains

scientific article

Molecular Basis of Histone H3K4me3 Recognition by ING4

scientific article

Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus

scientific article

Molecular mechanism of Gαi activation by non-GPCR proteins with a Gα-Binding and Activating motif

scientific article published on 18 May 2017

NMR assignment and backbone dynamics of the pore-forming domain of colicin A.

scientific article

NMR assignment and secondary structure of human growth arrest and DNA damage alpha protein (Gadd45 alpha)

scientific article published on 12 August 2008

Nucleotide binding triggers a conformational change of the CBS module of the magnesium transporter CNNM2 from a twisted towards a flat structure

scientific article published in November 2014

On the relevance of defining protein structures in cancer research.

scientific article published on April 2008

Production of meganucleases by cell-free protein synthesis for functional and structural studies.

scientific article published on 15 August 2012

Proliferating cell nuclear antigen (PCNA) interactions in solution studied by NMR

scientific article

Proliferating cell nuclear antigen structure and interactions: too many partners for one dancer?

scientific article published on January 2013

Rapid identification of fluorochrome modification sites in proteins by LC ESI-Q-TOF mass spectrometry

scientific article

Reduced stability and increased dynamics in the human proliferating cell nuclear antigen (PCNA) relative to the yeast homolog

scientific article

Solution structure of human growth arrest and DNA damage 45alpha (Gadd45alpha) and its interactions with proliferating cell nuclear antigen (PCNA) and Aurora A kinase

scientific article

Solution structure of the hypothetical protein Mth677 from Methanobacterium thermoautotrophicum: a novel alpha+beta fold

scientific article published on June 2004

Structural Analysis of the Black-Legged Tick Saliva Protein Salp15

scientific article published in 2022

Structural Insights into the Intracellular Region of the Human Magnesium Transport Mediator CNNM4 ⬇️

scientific article published on 12 December 2019

Structural basis of human PCNA sliding on DNA.

scientific article

Structure of p15(PAF)-PCNA complex and implications for clamp sliding during DNA replication and repair

scientific article

Structure of the I-SceI nuclease complexed with its dsDNA target and three catalytic metal ions.

scientific article published on 23 May 2016

Structure of the processive human Pol δ holoenzyme

scientific article published on 28 February 2020

TRAIP is a PCNA-binding ubiquitin ligase that protects genome stability after replication stress

scientific article published on 28 December 2015

The C-terminal RNA binding motif of HuR is a multi-functional domain leading to HuR oligomerization and binding to U-rich RNA targets.

scientific article

The Gαi-GIV binding interface is a druggable protein-protein interaction

scientific article published on 17 August 2017

The NMR structure and dynamics of the two-domain tick carboxypeptidase inhibitor reveal flexibility in its free form and stiffness upon binding to human carboxypeptidase B

scientific article

The NMR structures of the major intermediates of the two-domain tick carboxypeptidase inhibitor reveal symmetry in its folding and unfolding pathways

scientific article

The Tumor Suppressor ING5 Is a Dimeric, Bivalent Recognition Molecule of the Histone H3K4me3 Mark

scientific article published on 24 April 2019

The dimeric structure and the bivalent recognition of H3K4me3 by the tumor suppressor ING4 suggests a mechanism for enhanced targeting of the HBO1 complex to chromatin.

scientific article

The family 52 beta-xylosidase from Geobacillus stearothermophilus is a dimer: structural and biophysical characterization of a glycoside hydrolase

scientific article published on 08 July 2008

The heterotrimeric laminin coiled-coil domain exerts anti-adhesive effects and induces a pro-invasive phenotype

scientific article

The hydrophobic-staple motif and a role for loop-residues in α-helix stability and protein folding ⬇️

scientific article published on May 1, 1995

The immunosuppressive effect of the tick protein, Salp15, is long-lasting and persists in a murine model of hematopoietic transplant

scientific article published on 6 September 2017

The metastasis suppressor KISS1 is an intrinsically disordered protein slightly more extended than a random coil

scientific article

The p12 subunit of human polymerase δ uses an atypical PIP box for molecular recognition of proliferating cell nuclear antigen (PCNA)

scientific article published on 17 January 2019

The tumor suppressor ING1 contributes to epigenetic control of cellular senescence

The tumor suppressor inhibitor of growth 4 binds double-stranded DNA through its disordered central region

scientific article published on 7 December 2016

Transient DNA / RNA-protein interactions.

scientific article published on 30 March 2011

Trispecific T-cell engagers for dual tumor-targeting of colorectal cancer

scientific article published on 07 February 2022

Understanding the indirect DNA read-out specificity of I-CreI Meganuclease

scientific article published in Scientific Reports

Utilization of I-domain of LFA-1 to Target Drug and Marker Molecules to Leukocytes.

scientific article

XTACC3-XMAP215 association reveals an asymmetric interaction promoting microtubule elongation

scientific article

p15PAF binding to PCNA modulates the DNA sliding surface

scientific article published on 01 October 2018

p15PAF is an intrinsically disordered protein with nonrandom structural preferences at sites of interaction with other proteins.

scientific article

List of works by Francisco J. Blanco

1H and 15N NMR assignment and solution structure of the SH3 domain of spectrin: comparison of unrefined and refined structure sets with the crystal structure

2D-NMR reveals different populations of exposed lysine residues in the apoB-100 protein of electronegative and electropositive fractions of LDL particles

A generalized approach for NMR studies of lipid–protein interactions based on sparse fluorination of acyl chains

A tumor-targeted trimeric 4-1BB-agonistic antibody induces potent anti-tumor immunity without systemic toxicity

ATTACK, a novel bispecific T cell-recruiting antibody with trivalent EGFR binding and monovalent CD3 binding for cancer immunotherapy

Aggregated electronegative low density lipoprotein in human plasma shows a high tendency toward phospholipolysis and particle fusion

Backbone assignment of human proliferating cell nuclear antigen.

Backbone assignment of the 98 kDa homotrimeric yeast PCNA ring

Bacterial secretion of soluble and functional trivalent scFv-based N-terminal trimerbodies.

Binding site plasticity in viral PPxY Late domain recognition by the third WW domain of human NEDD4

Carcinoembryonic Antigen (CEA)-Specific 4-1BB-Costimulation Induced by CEA-Targeted 4-1BB-Agonistic Trimerbodies

Crystal Structure of Inhibitor of Growth 4 (ING4) Dimerization Domain Reveals Functional Organization of ING Family of Chromatin-binding Proteins

Crystal Structure of the Homing Endonuclease I-CvuI Provides a New Template for Genome Modification.

Crystal structure of I-DmoI in complex with its target DNA provides new insights into meganuclease engineering

Crystal structure of the third extracellular domain of CD5 reveals the fold of a group B scavenger cysteine-rich receptor domain

Crystallization and preliminary X-ray diffraction analysis of the dimerization domain of the tumour suppressor ING4.

Crystallization and preliminary X-ray diffraction analysis of the homing endonuclease I-CvuI from Chlorella vulgaris in complex with its target DNA.

Crystallization and preliminary X-ray diffraction analysis on the homing endonuclease I-Dmo-I in complex with its target DNA.

Design, synthesis, and functional evaluation of leukocyte function associated antigen-1 antagonists in early and late stages of cancer development.

Disentangling polydispersity in the PCNA-p15PAF complex, a disordered, transient and multivalent macromolecular assembly.

Double Monoubiquitination Modifies the Molecular Recognition Properties of p15PAF Promoting Binding to the Reader Module of Dnmt1

Efficient production of single-chain fragment variable-based N-terminal trimerbodies in Pichia pastoris

Efficient targeting of a SCID gene by an engineered single-chain homing endonuclease

Engineering of large numbers of highly specific homing endonucleases that induce recombination on novel DNA targets

Enhanced antiangiogenic therapy with antibody-collagen XVIII NC1 domain fusion proteins engineered to exploit matrix remodeling events.

Evolution of SUMO Function and Chain Formation in Insects.

Expression, purification and crystallization of human CD5 domain III, a nano-scale crystallization example.

Folding and aggregation kinetics of a beta-hairpin

Folding of Protein G B1 Domain Studied by the Conformational Characterization of Fragments Comprising Its Secondary Structure Elements ⬇️

Generation and characterization of monospecific and bispecific hexavalent trimerbodies

Homing endonucleases: from basics to therapeutic applications.

ICAM-1 peptide inhibitors of T-cell adhesion bind to the allosteric site of LFA-1. An NMR characterization

Impaired Function of Solute Carrier Family 19 Leads to Low Folate Levels and Lipid Droplet Accumulation in Hepatocytes

Improved stability of multivalent antibodies containing the human collagen XV trimerization domain.

In vivo tumor targeting and imaging with engineered trivalent antibody fragments containing collagen-derived sequences

Intramolecular trimerization, a novel strategy for making multispecific antibodies with controlled orientation of the antigen binding domains

Molecular Basis of Histone H3K4me3 Recognition by ING4

Molecular basis of engineered meganuclease targeting of the endogenous human RAG1 locus

Molecular mechanism of Gαi activation by non-GPCR proteins with a Gα-Binding and Activating motif

NMR assignment and backbone dynamics of the pore-forming domain of colicin A.

NMR assignment and secondary structure of human growth arrest and DNA damage alpha protein (Gadd45 alpha)

Nucleotide binding triggers a conformational change of the CBS module of the magnesium transporter CNNM2 from a twisted towards a flat structure

On the relevance of defining protein structures in cancer research.

Production of meganucleases by cell-free protein synthesis for functional and structural studies.

Proliferating cell nuclear antigen (PCNA) interactions in solution studied by NMR

Proliferating cell nuclear antigen structure and interactions: too many partners for one dancer?

Rapid identification of fluorochrome modification sites in proteins by LC ESI-Q-TOF mass spectrometry

Reduced stability and increased dynamics in the human proliferating cell nuclear antigen (PCNA) relative to the yeast homolog

Solution structure of human growth arrest and DNA damage 45alpha (Gadd45alpha) and its interactions with proliferating cell nuclear antigen (PCNA) and Aurora A kinase

Solution structure of the hypothetical protein Mth677 from Methanobacterium thermoautotrophicum: a novel alpha+beta fold

Structural Analysis of the Black-Legged Tick Saliva Protein Salp15

Structural Insights into the Intracellular Region of the Human Magnesium Transport Mediator CNNM4 ⬇️

Structural basis of human PCNA sliding on DNA.

Structure of p15(PAF)-PCNA complex and implications for clamp sliding during DNA replication and repair

Structure of the I-SceI nuclease complexed with its dsDNA target and three catalytic metal ions.

Structure of the processive human Pol δ holoenzyme

TRAIP is a PCNA-binding ubiquitin ligase that protects genome stability after replication stress

The C-terminal RNA binding motif of HuR is a multi-functional domain leading to HuR oligomerization and binding to U-rich RNA targets.

The Gαi-GIV binding interface is a druggable protein-protein interaction

The NMR structure and dynamics of the two-domain tick carboxypeptidase inhibitor reveal flexibility in its free form and stiffness upon binding to human carboxypeptidase B

The NMR structures of the major intermediates of the two-domain tick carboxypeptidase inhibitor reveal symmetry in its folding and unfolding pathways

The Tumor Suppressor ING5 Is a Dimeric, Bivalent Recognition Molecule of the Histone H3K4me3 Mark

The dimeric structure and the bivalent recognition of H3K4me3 by the tumor suppressor ING4 suggests a mechanism for enhanced targeting of the HBO1 complex to chromatin.

The family 52 beta-xylosidase from Geobacillus stearothermophilus is a dimer: structural and biophysical characterization of a glycoside hydrolase

The heterotrimeric laminin coiled-coil domain exerts anti-adhesive effects and induces a pro-invasive phenotype

The hydrophobic-staple motif and a role for loop-residues in α-helix stability and protein folding ⬇️

The immunosuppressive effect of the tick protein, Salp15, is long-lasting and persists in a murine model of hematopoietic transplant

The metastasis suppressor KISS1 is an intrinsically disordered protein slightly more extended than a random coil

The p12 subunit of human polymerase δ uses an atypical PIP box for molecular recognition of proliferating cell nuclear antigen (PCNA)

The tumor suppressor ING1 contributes to epigenetic control of cellular senescence

The tumor suppressor inhibitor of growth 4 binds double-stranded DNA through its disordered central region

Transient DNA / RNA-protein interactions.

Trispecific T-cell engagers for dual tumor-targeting of colorectal cancer

Understanding the indirect DNA read-out specificity of I-CreI Meganuclease

Utilization of I-domain of LFA-1 to Target Drug and Marker Molecules to Leukocytes.

XTACC3-XMAP215 association reveals an asymmetric interaction promoting microtubule elongation

p15PAF binding to PCNA modulates the DNA sliding surface

p15PAF is an intrinsically disordered protein with nonrandom structural preferences at sites of interaction with other proteins.