List of works - Carlos Barbas

3-Pyrrolidinecarboxylic acid for direct catalytic asymmetric anti-Mannich-type reactions of unmodified ketones

scientific article

A highly enantioselective amino acid-catalyzed route to functionalized alpha-amino acids

scientific article published in March 2002

A highly enantioselective route to either enantiomer of both alpha- and beta-amino acid derivatives

scientific article

A modular assembly strategy for improving the substrate specificity of small catalytic peptides

scientific article

A new visual screening assay for catalytic antibodies with retro-aldol retro-Michael activity.

scientific article published on 22 December 2006

A zinc-finger transcriptional activator designed to interact with the gamma-globin gene promoters enhances fetal hemoglobin production in primary human adult erythroblasts

scientific article

Aldolase antibody activation of prodrugs of potent aldehyde-containing cytotoxics for selective chemotherapy

scientific article published on October 2004

Amine-catalyzed Michael reactions of an aminoaldehyde derivative to nitroolefins

Amine-catalyzed direct Diels–Alder reactions of α,β-unsaturated ketones with nitro olefins

An efficient chemical approach to bispecific antibodies and antibodies of high valency

scientific article

Antibody conjugation approach enhances breadth and potency of neutralization of anti-HIV-1 antibodies and CD4-IgG

scientific article published on 20 February 2013

Antibody-catalyzed Aldol Reactions

Antibody-catalyzed aminolysis of a chloropyrimidine derivative

scientific article

Antibody-catalyzed asymmetric intramolecular Michael addition of aldehydes and ketones to yield the disfavored cis-product

scientific article published on 01 September 2005

Assembly of spirooxindole derivatives containing four consecutive stereocenters via organocatalytic Michael-Henry cascade reactions

scientific article published on 22 March 2012

Assembly of spirooxindole derivatives via organocatalytic iminium-enamine cascade reactions

scientific article published on 14 November 2012

Asymmetric construction of spirocyclopentenebenzofuranone core structures via highly selective phosphine-catalyzed [3 + 2] cycloaddition reactions.

scientific article published on 3 June 2013

Asymmetric synthesis of quaternary alpha- and beta-amino acids and beta-lactams via proline-catalyzed mannich reactions with branched aldehyde donors

scientific article published on 01 July 2004

Attenuation of HIV-1 replication in primary human cells with a designed zinc finger transcription factor.

scientific article

Breaking the one antibody-one target axiom

scholarly article

Catalysis of 3-pyrrolidinecarboxylic acid and related pyrrolidine derivatives in enantioselective anti-Mannich-type reactions: importance of the 3-acid group on pyrrolidine for stereocontrol

scientific article published on 29 December 2007

Catalytic single-chain antibodies possessing β-lactamase activity selected from a phage displayed combinatorial library using a mechanism-based inhibitor

Cell-penetrating peptide-mediated delivery of TALEN proteins via bioconjugation for genome engineering

scientific article (publication date: 2014)

Chemical adaptor immunotherapy: design, synthesis, and evaluation of novel integrin-targeting devices.

scientific article

Chemically Programmed Antibodies AS HIV-1 Attachment Inhibitors

scientific article published on May 2013

Chemically programmed monoclonal antibodies for cancer therapy: adaptor immunotherapy based on a covalent antibody catalyst.

scientific article

Construction of bispirooxindoles containing three quaternary stereocentres in a cascade using a single multifunctional organocatalyst.

scientific article published on 8 May 2011

Core structure-based design of organocatalytic [3+2]-cycloaddition reactions: highly efficient and stereocontrolled syntheses of 3,3'-pyrrolidonyl spirooxindoles

scientific article published on 09 December 2011

Core-structure-motivated design of a phosphine-catalyzed [3+2] cycloaddition reaction: enantioselective syntheses of spirocyclopenteneoxindoles.

scientific article published on 11 March 2011

Crystallization and preliminary X-ray crystallographic analysis of Aart, a designed six-finger zinc-finger peptide, bound to DNA.

scientific article

Custom zinc-finger nucleases for use in human cells

scientific article published in October 2005

De novo computational design of retro-aldol enzymes

scientific article

Design and use of fluorogenic aldehydes for monitoring the progress of aldehyde transformations

scientific article published in March 2004

Designing transcription factor architectures for drug discovery.

scientific article published on 31 August 2004

Determination of cysteine concentration by fluorescence increase: reaction of cysteine with a fluorogenic aldehyde

scientific article

Development of a small peptide tag for covalent labeling of proteins

scientific article

Development of functional human monoclonal single-chain variable fragment antibody against HIV-1 from human cervical B cells

scientific article published on 01 April 2003

Development of small designer aldolase enzymes: catalytic activity, folding, and substrate specificity

scientific article

Development of zinc finger domains for recognition of the 5'-CNN-3' family DNA sequences and their use in the construction of artificial transcription factors

scientific article

Dihydroxyacetone variants in the organocatalytic construction of carbohydrates: mimicking tagatose and fuculose aldolases

scientific article published in May 2006

Dimeric quinidine-catalyzed enantioselective aminooxygenation of oxindoles: an organocatalytic approach to 3-hydroxyoxindole derivatives.

scientific article published in April 2010

Direct Asymmetric Organocatalytic Michael Reactions of α,α-Disubstituted Aldehydes with β-Nitrostyrenes for the Synthesis of Quaternary Carbon-Containing Products

scientific article published in 2004

Direct Observation of an Enamine Intermediate in Amine Catalysis

scientific article

Direct Organocatalytic Asymmetric Aldol Reactions of α-Amino Aldehydes: Expedient Syntheses of Highly Enantiomerically Enrichedanti-β-Hydroxy-α-amino Acids

scientific article published in 2004

Direct amino acid-catalyzed asymmetric desymmetrization of meso-compounds: tandem aminoxylation/O-N bond heterolysis reactions

scientific article published on 01 April 2005

Direct asymmetric alpha-fluorination of aldehydes

scientific article published on 01 June 2005

Direct asymmetric anti-Mannich-type reactions catalyzed by a designed amino acid

scientific article

Direct catalytic asymmetric synthesis of anti-1,2-amino alcohols and syn-1,2-diols through organocatalytic anti-Mannich and syn-aldol reactions

scientific article published in January 2007

Direct organocatalytic aldol reactions in buffered aqueous media

scientific article

Direct organocatalytic asymmetric heterodomino reactions: the Knoevenagel/Diels-Alder/epimerization sequence for the highly diastereoselective synthesis of symmetrical and nonsymmetrical synthons of benzoannelated centropolyquinanes

scientific article

Directed evolution of an enhanced and highly efficient FokI cleavage domain for zinc finger nucleases

scientific article

Directed evolution of recombinase specificity by split gene reassembly

scientific article

Efficient delivery of nuclease proteins for genome editing in human stem cells and primary cells

scientific article published on 22 October 2015

Enamine Catalysis: Aldol and Mannich-Type Reactions

Enamine-based organocatalysis with proline and diamines: the development of direct catalytic asymmetric Aldol, Mannich, Michael, and Diels-alder reactions

scientific article

Engineering polydactyl zinc-finger transcription factors

scientific article (publication date: February 2002)

Evaluation of a modular strategy for the construction of novel polydactyl zinc finger DNA-binding proteins

scientific article

Evolution of aldolase antibodies in vitro: correlation of catalytic activity and reaction-based selection

scientific article

Evolution of programmable zinc finger-recombinases with activity in human cells.

scientific article published on 12 January 2007

Expanding the scope of cinchona alkaloid-catalyzed enantioselective alpha-aminations of oxindoles: a versatile approach to optically active 3-amino-2-oxindole derivatives

scientific article published in December 2009

Expedient Synthesis of Chiral 1,2- and 1,4-Diamines: Protecting Group Dependent Regioselectivity in Direct Organocatalytic Asymmetric Mannich Reactions

article published in 2006

Exploring strategies for the design of artificial transcription factors: targeting sites proximal to known regulatory regions for the induction of gamma-globin expression and the treatment of sickle cell disease

scientific article

Facile and stabile linkages through tyrosine: bioconjugation strategies with the tyrosine-click reaction

scientific article published on 27 March 2013

Flow cytometric screening of aldolase catalytic antibodies

scientific article

Fluorescent detection of carbon-carbon bond formation

scientific article published in July 2003

Formylbenzene diazonium hexafluorophosphate reagent for tyrosine-selective modification of proteins and the introduction of a bioorthogonal aldehyde

scientific article

Functional neutralization of HIV-1 Vif protein by intracellular immunization inhibits reverse transcription and viral replication

scientific article

Fusion proteins consisting of human immunodeficiency virus type 1 integrase and the designed polydactyl zinc finger protein E2C direct integration of viral DNA into specific sites

scientific article published on February 2004

Generation and functional characterization of intracellular antibodies interacting with the kinase domain of human EGF receptor

scientific article published in March 2003

Generation of human Fab antibody libraries: PCR amplification and assembly of light- and heavy-chain coding sequences.

scientific article

Generation of human scFv antibody libraries: PCR amplification and assembly of light- and heavy-chain coding sequences.

scientific article

Genetic reprogramming of tumor cells by zinc finger transcription factors

scientific article

Highly efficient hydrogen-bonding catalysis of the Diels-Alder reaction of 3-vinylindoles and methyleneindolinones provides carbazolespirooxindole skeletons.

scientific article published on 22 July 2011

Highly enantio- and diastereoselective Mannich reactions of glycine Schiff bases with in situ generated N-Boc-imines catalyzed by a cinchona alkaloid thiourea

scientific article published in February 2010

Highly enantioselective organocatalytic α-amination reactions of aryl oxindoles: developing designer multifunctional alkaloid catalysts

scientific article published on 11 November 2010

Human Serum Albumin Domain I Fusion Protein for Antibody Conjugation.

scientific article published on 26 September 2016

Human immunodeficiency virus type 1 incorporated with fusion proteins consisting of integrase and the designed polydactyl zinc finger protein E2C can bias integration of viral DNA into a predetermined chromosomal region in human cells

scientific article

Human/mouse cross-reactive anti-VEGF receptor 2 recombinant antibodies selected from an immune b9 allotype rabbit antibody library.

scientific article

Improved cell-penetrating zinc-finger nuclease proteins for precision genome engineering

scientific article

Improving the serum stability of site-specific antibody conjugates with sulfone linkers

scientific article

In vivo programming of endogenous antibodies via oral administration of adaptor ligands

scientific article

In vivo selection of combinatorial libraries and designed affinity maturation of polydactyl zinc finger transcription factors for ICAM-1 provides new insights into gene regulation

scientific article

In water, on water, and by water: mimicking nature's aldolases with organocatalysis and water

scientific article published on 09 July 2010

Induction of Fetal Hemoglobin In Vivo Mediated by a Synthetic γ-Globin Zinc Finger Activator

scientific article published on 15 June 2012

Inhibition of human immunodeficiency virus type 1 replication with artificial transcription factors targeting the highly conserved primer-binding site

scientific article

Instant immunity through chemically programmable vaccination and covalent self-assembly

scholarly article

Integrin alpha(v)beta3 targeted therapy for Kaposi's sarcoma with an in vitro evolved antibody

scientific article

Integrin alphaIIbbeta3-specific synthetic human monoclonal antibodies and HCDR3 peptides that potently inhibit platelet aggregation

scientific article published on 19 December 2003

Intrabodies targeting the Kaposi sarcoma-associated herpesvirus latency antigen inhibit viral persistence in lymphoma cells.

scientific article

Intradiabodies, bispecific, tetravalent antibodies for the simultaneous functional knockout of two cell surface receptors.

scientific article

Isolation of human prostate cancer cell reactive antibodies using phage display technology.

scientific article

Maspin alters the carcinoma proteome

scientific article

Mimicking Aldolases through Organocatalysis: syn-Selective Aldol Reactions with Protected Dihydroxyacetone

scientific article published in 2007

Mimicking dihydroxy acetone phosphate-utilizing aldolases through organocatalysis: a facile route to carbohydrates and aminosugars

scientific article published on 01 March 2005

Mimicking fructose and rhamnulose aldolases: organocatalytic syn-aldol reactions with unprotected dihydroxyacetone

scientific article published in January 2007

Modular system for the construction of zinc-finger libraries and proteins

scientific article

Modulation of drug resistance by artificial transcription factors.

scientific article

Negative regulation of the RTBV promoter by designed zinc finger proteins

scientific article published on 19 February 2010

Neutralization of Burkholderia pseudomallei protease by Fabs generated through phage display

scientific article

One-Pot Asymmetric Synthesis of β-Cyanohydroxymethyl α-Amino Acid Derivatives: Formation of Three Contiguous Stereogenic Centers

scientific article published on 01 December 2002

One-Pot Enantioselective Syntheses of Iminosugar Derivatives Using Organocatalytic anti-Michael−anti-Aza-Henry Reactions ⬇️

scientific article published on October 29, 2010

Organocatalysis as a safe practical method for the stereospecific dibromination of unsaturated compounds

scientific article published on 23 March 2012

Organocatalytic Asymmetric Assembly Reactions: One-Pot Synthesis of Functionalized β-Amino Alcohols from Aldehydes, Ketones, and Azodicarboxylates ⬇️

scientific article published on May 15, 2003

Organocatalytic Asymmetric Domino Knoevenagel/Diels–Alder Reactions: A Bioorganic Approach to the Diastereospecific and Enantioselective Construction of Highly Substituted Spiro[5,5]undecane‐1,5,9‐triones ⬇️

scientific article published on September 15, 2003

Organocatalytic Direct Michael Reaction of Ketones and Aldehydes with β-Nitrostyrene in Brine [J. Am. Chem. Soc.2006,128, 4966−4967]

scientific article published in 2006

Organocatalytic amidation and esterification of aldehydes with activating reagents by a cross-coupling strategy

scientific article published on 07 November 2012

Organocatalytic asymmetric assembly reactions for the syntheses of carbohydrate derivatives by intermolecular Michael-Henry reactions

scientific article

Organocatalytic direct asymmetric aldol reactions in water

scientific article

Organocatalytic direct michael reaction of ketones and aldehydes with beta-nitrostyrene in brine

scientific article

Organocatalytic enantioselective synthesis of metabotropic glutamate receptor ligands

scientific article published on 01 September 2005

Organocatalytic mannich-type reactions of trifluoroethyl thioesters

scientific article published on 16 July 2008

Phenotypic knockout of VEGF-R2 and Tie-2 with an intradiabody reduces tumor growth and angiogenesis in vivo

scientific article

Pipecolic acid-catalyzed direct asymmetric mannich reactions

scientific article

Positive selection of DNA-protein interactions in mammalian cells through phenotypic coupling with retrovirus production

scientific article

Potent Inhibition of HIV‐1 Entry with a Chemically Programmed Antibody Aided by an Efficient Organocatalytic Synthesis ⬇️

scientific article published on October 18, 2010

Preparation and activities of macromolecule conjugates of the CCR5 antagonist Maraviroc

scientific article published on February 2014

Preparation of integrin alpha(v)beta3-targeting Ab 38C2 constructs.

scientific article

Prodrug activation gated by a molecular "OR" logic trigger

scientific article published on July 2005

Proline-Catalyzed Direct Asymmetric Aldol Reactions

scientific article (publication date: March 2000)

Proline-catalyzed one-step asymmetric synthesis of 5-hydroxy-(2E)-hexenal from acetaldehyde.

scientific article published in January 2002

Promoter-targeted phage display selections with preassembled synthetic zinc finger libraries for endogenous gene regulation.

scientific article

Protein delivery using Cys2-His2 zinc-finger domains

scientific article published on 19 June 2014

Quantitation of DNA and RNA

scientific article published on 01 November 2007

Rabbit immune repertoires as sources for therapeutic monoclonal antibodies: the impact of kappa allotype-correlated variation in cysteine content on antibody libraries selected by phage display

scientific article

Rapid Fluorescent Screening for Bifunctional Amine−Acid Catalysts: Efficient Syntheses of Quaternary Carbon-Containing Aldols under Organocatalysis

article published in 2003

Rapid Monoclonal Antibody Generation Via Dendritic Cell TargetingIn Vivo ⬇️

scientific article published on February 1, 2003

Rapid analysis of solvent effects on enamine formation by fluorescence: how might enzymes facilitate enamine chemistry with primary amines?

Rapid, stable, chemoselective labeling of thiols with Julia-Kocieński-like reagents: a serum-stable alternative to maleimide-based protein conjugation

scientific article published on 02 October 2013

Rationally designed amide donors for organocatalytic asymmetric Michael reactions

scientific article published on 13 April 2012

Reactivation of Latent HIV-1 Expression by Engineered TALE Transcription Factors.

scientific article

Reactive Immunization: A Unique Approach to Aldolase Antibodies

Reactive immunization: a unique approach to catalytic antibodies

scientific article

Recombinant rabbit single-chain antibodies bind to the catalytic and C-terminal domains of HIV-1 integrase protein and strongly inhibit HIV-1 replication

scientific article

Reconstructing Aldolase Antibodies to Alter Their Substrate Specificity and Turnover

article published in 2000

Redesigning Recombinase Specificity for Safe Harbor Sites in the Human Genome

scientific article published on 28 September 2015

Scanning the human genome with combinatorial transcription factor libraries.

scientific article

Selection of phage-displayed peptides that bind to a particular ligand-bound antibody

scientific article

Single-triggered trimeric prodrugs.

scientific article

Site-selective labeling of a lysine residue in human serum albumin.

scientific article published on 4 September 2014

Small molecule drug activity in melanoma models may be dramatically enhanced with an antibody effector

scientific article published on 01 September 2006

Structure of Aart, a designed six-finger zinc finger peptide, bound to DNA.

scientific article published on 11 August 2006

Synthesis of beta-hydroxyaldehydes with stereogenic quaternary carbon centers by direct organocatalytic asymmetric aldol reactions

scientific article published in April 2004

Synthesis of programmable integrases

scholarly article

Synthesis of the next-generation therapeutic antibodies that combine cell targeting and antibody-catalyzed prodrug activation.

scholarly article

Synthesis ofβ-Hydroxyaldehydes with Stereogenic Quaternary Carbon Centers by Direct Organocatalytic Asymmetric Aldol Reactions

article published in 2004

Synthetic zinc finger proteins: the advent of targeted gene regulation and genome modification technologies

scientific article

Targeted gene knockout by direct delivery of zinc-finger nuclease proteins

scientific article

Targeting tumor angiogenesis with adenovirus-delivered anti-Tie-2 intrabody

scientific article published on 01 February 2005

The ErbB2/ErbB3 heterodimer functions as an oncogenic unit: ErbB2 requires ErbB3 to drive breast tumor cell proliferation

scientific article

The direct organocatalytic asymmetric mannich reaction: unmodified aldehydes as nucleophiles

scientific article

The origin of enantioselectivity in aldolase antibodies: crystal structure, site-directed mutagenesis, and computational analysis

scientific article published in November 2004

Thiourea-catalyzed highly enantio- and diastereoselective additions of oxindoles to nitroolefins: application to the formal synthesis of (+)-physostigmine.

scientific article published in July 2009

Total synthesis of LFA-1 antagonist BIRT-377 via organocatalytic asymmetric construction of a quaternary stereocenter

scientific article published on 01 March 2005

Towards organocatalytic polyketide synthases with diverse electrophile scope: trifluoroethyl thioesters as nucleophiles in organocatalytic Michael reactions and beyond

scientific article published in January 2008

Translating the genomics revolution: the need for an international gene therapy consortium for monogenic diseases

scientific article published in February 2013

Tyrosine bioconjugation through aqueous ene-type reactions: a click-like reaction for tyrosine

scientific article published in February 2010

Water-compatible organocatalysts for direct asymmetric syn-aldol reactions of dihydroxyacetone and aldehydes

scientific article published on 20 March 2008

Zinc finger transcription factors designed for bispecific coregulation of ErbB2 and ErbB3 receptors: insights into ErbB receptor biology

scientific article published on October 2005

anti-Selective asymmetric Michael reactions of aldehydes and nitroolefins catalyzed by a primary amine/thiourea

scientific article published in January 2009

{gamma}-Globin gene expression in chemical inducer of dimerization (CID)-dependent multipotential cells established from human {beta}-globin locus yeast artificial chromosome ({beta}-YAC) transgenic mice

scientific article

List of works by Carlos Barbas

3-Pyrrolidinecarboxylic acid for direct catalytic asymmetric anti-Mannich-type reactions of unmodified ketones

A highly enantioselective amino acid-catalyzed route to functionalized alpha-amino acids

A highly enantioselective route to either enantiomer of both alpha- and beta-amino acid derivatives

A modular assembly strategy for improving the substrate specificity of small catalytic peptides

A new visual screening assay for catalytic antibodies with retro-aldol retro-Michael activity.

A zinc-finger transcriptional activator designed to interact with the gamma-globin gene promoters enhances fetal hemoglobin production in primary human adult erythroblasts

Aldolase antibody activation of prodrugs of potent aldehyde-containing cytotoxics for selective chemotherapy

Amine-catalyzed Michael reactions of an aminoaldehyde derivative to nitroolefins

Amine-catalyzed direct Diels–Alder reactions of α,β-unsaturated ketones with nitro olefins

An efficient chemical approach to bispecific antibodies and antibodies of high valency

Antibody conjugation approach enhances breadth and potency of neutralization of anti-HIV-1 antibodies and CD4-IgG

Antibody-catalyzed Aldol Reactions

Antibody-catalyzed aminolysis of a chloropyrimidine derivative

Antibody-catalyzed asymmetric intramolecular Michael addition of aldehydes and ketones to yield the disfavored cis-product

Assembly of spirooxindole derivatives containing four consecutive stereocenters via organocatalytic Michael-Henry cascade reactions

Assembly of spirooxindole derivatives via organocatalytic iminium-enamine cascade reactions

Asymmetric construction of spirocyclopentenebenzofuranone core structures via highly selective phosphine-catalyzed [3 + 2] cycloaddition reactions.

Asymmetric synthesis of quaternary alpha- and beta-amino acids and beta-lactams via proline-catalyzed mannich reactions with branched aldehyde donors

Attenuation of HIV-1 replication in primary human cells with a designed zinc finger transcription factor.

Breaking the one antibody-one target axiom

Catalysis of 3-pyrrolidinecarboxylic acid and related pyrrolidine derivatives in enantioselective anti-Mannich-type reactions: importance of the 3-acid group on pyrrolidine for stereocontrol

Catalytic single-chain antibodies possessing β-lactamase activity selected from a phage displayed combinatorial library using a mechanism-based inhibitor

Cell-penetrating peptide-mediated delivery of TALEN proteins via bioconjugation for genome engineering

Chemical adaptor immunotherapy: design, synthesis, and evaluation of novel integrin-targeting devices.

Chemically Programmed Antibodies AS HIV-1 Attachment Inhibitors

Chemically programmed monoclonal antibodies for cancer therapy: adaptor immunotherapy based on a covalent antibody catalyst.

Construction of bispirooxindoles containing three quaternary stereocentres in a cascade using a single multifunctional organocatalyst.

Core structure-based design of organocatalytic [3+2]-cycloaddition reactions: highly efficient and stereocontrolled syntheses of 3,3'-pyrrolidonyl spirooxindoles

Core-structure-motivated design of a phosphine-catalyzed [3+2] cycloaddition reaction: enantioselective syntheses of spirocyclopenteneoxindoles.

Crystallization and preliminary X-ray crystallographic analysis of Aart, a designed six-finger zinc-finger peptide, bound to DNA.

Custom zinc-finger nucleases for use in human cells

De novo computational design of retro-aldol enzymes

Design and use of fluorogenic aldehydes for monitoring the progress of aldehyde transformations

Designing transcription factor architectures for drug discovery.

Determination of cysteine concentration by fluorescence increase: reaction of cysteine with a fluorogenic aldehyde

Development of a small peptide tag for covalent labeling of proteins

Development of functional human monoclonal single-chain variable fragment antibody against HIV-1 from human cervical B cells

Development of small designer aldolase enzymes: catalytic activity, folding, and substrate specificity

Development of zinc finger domains for recognition of the 5'-CNN-3' family DNA sequences and their use in the construction of artificial transcription factors

Dihydroxyacetone variants in the organocatalytic construction of carbohydrates: mimicking tagatose and fuculose aldolases

Dimeric quinidine-catalyzed enantioselective aminooxygenation of oxindoles: an organocatalytic approach to 3-hydroxyoxindole derivatives.

Direct Asymmetric Organocatalytic Michael Reactions of α,α-Disubstituted Aldehydes with β-Nitrostyrenes for the Synthesis of Quaternary Carbon-Containing Products

Direct Observation of an Enamine Intermediate in Amine Catalysis

Direct Organocatalytic Asymmetric Aldol Reactions of α-Amino Aldehydes: Expedient Syntheses of Highly Enantiomerically Enrichedanti-β-Hydroxy-α-amino Acids

Direct amino acid-catalyzed asymmetric desymmetrization of meso-compounds: tandem aminoxylation/O-N bond heterolysis reactions

Direct asymmetric alpha-fluorination of aldehydes

Direct asymmetric anti-Mannich-type reactions catalyzed by a designed amino acid

Direct catalytic asymmetric synthesis of anti-1,2-amino alcohols and syn-1,2-diols through organocatalytic anti-Mannich and syn-aldol reactions

Direct organocatalytic aldol reactions in buffered aqueous media

Direct organocatalytic asymmetric heterodomino reactions: the Knoevenagel/Diels-Alder/epimerization sequence for the highly diastereoselective synthesis of symmetrical and nonsymmetrical synthons of benzoannelated centropolyquinanes

Directed evolution of an enhanced and highly efficient FokI cleavage domain for zinc finger nucleases

Directed evolution of recombinase specificity by split gene reassembly

Efficient delivery of nuclease proteins for genome editing in human stem cells and primary cells

Enamine Catalysis: Aldol and Mannich-Type Reactions

Enamine-based organocatalysis with proline and diamines: the development of direct catalytic asymmetric Aldol, Mannich, Michael, and Diels-alder reactions

Engineering polydactyl zinc-finger transcription factors

Evaluation of a modular strategy for the construction of novel polydactyl zinc finger DNA-binding proteins

Evolution of aldolase antibodies in vitro: correlation of catalytic activity and reaction-based selection

Evolution of programmable zinc finger-recombinases with activity in human cells.

Expanding the scope of cinchona alkaloid-catalyzed enantioselective alpha-aminations of oxindoles: a versatile approach to optically active 3-amino-2-oxindole derivatives

Expedient Synthesis of Chiral 1,2- and 1,4-Diamines: Protecting Group Dependent Regioselectivity in Direct Organocatalytic Asymmetric Mannich Reactions

Exploring strategies for the design of artificial transcription factors: targeting sites proximal to known regulatory regions for the induction of gamma-globin expression and the treatment of sickle cell disease

Facile and stabile linkages through tyrosine: bioconjugation strategies with the tyrosine-click reaction

Flow cytometric screening of aldolase catalytic antibodies

Fluorescent detection of carbon-carbon bond formation

Formylbenzene diazonium hexafluorophosphate reagent for tyrosine-selective modification of proteins and the introduction of a bioorthogonal aldehyde

Functional neutralization of HIV-1 Vif protein by intracellular immunization inhibits reverse transcription and viral replication

Fusion proteins consisting of human immunodeficiency virus type 1 integrase and the designed polydactyl zinc finger protein E2C direct integration of viral DNA into specific sites

Generation and functional characterization of intracellular antibodies interacting with the kinase domain of human EGF receptor

Generation of human Fab antibody libraries: PCR amplification and assembly of light- and heavy-chain coding sequences.

Generation of human scFv antibody libraries: PCR amplification and assembly of light- and heavy-chain coding sequences.

Genetic reprogramming of tumor cells by zinc finger transcription factors

Highly efficient hydrogen-bonding catalysis of the Diels-Alder reaction of 3-vinylindoles and methyleneindolinones provides carbazolespirooxindole skeletons.

Highly enantio- and diastereoselective Mannich reactions of glycine Schiff bases with in situ generated N-Boc-imines catalyzed by a cinchona alkaloid thiourea

Highly enantioselective organocatalytic α-amination reactions of aryl oxindoles: developing designer multifunctional alkaloid catalysts

Human Serum Albumin Domain I Fusion Protein for Antibody Conjugation.

Human immunodeficiency virus type 1 incorporated with fusion proteins consisting of integrase and the designed polydactyl zinc finger protein E2C can bias integration of viral DNA into a predetermined chromosomal region in human cells

Human/mouse cross-reactive anti-VEGF receptor 2 recombinant antibodies selected from an immune b9 allotype rabbit antibody library.

Improved cell-penetrating zinc-finger nuclease proteins for precision genome engineering