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List of works by Stephen Bustin

A MIQE-compliant real-time PCR assay for Aspergillus detection

scientific article (publication date: 2012)

Age and diet act through distinct isoforms of the class II transactivator gene in mouse intestinal epithelium.

scientific article published in July 2004

Altered monocyte cyclo-oxygenase response in non-obese diabetic mice

scientific article published on 24 November 2008

Altered monocyte cyclooxygenase response to lipopolysaccharide in type 1 diabetes

scientific article published on December 2006

Aspergillus-Specific Lateral-Flow Device and Real-Time PCR Testing of Bronchoalveolar Lavage Fluid: a Combination Biomarker Approach for Clinical Diagnosis of Invasive Pulmonary Aspergillosis.

scientific article published on 22 April 2015

Avian influenza: virology, diagnosis and surveillance.

scientific article

Biomarkers for invasive aspergillosis: the challenges continue

scientific article

Coding sequence of ERF-1, the human homologue of Tis11b/cMG1, members of the Tis11 family of early response genes

scientific article

Colorectal cancers with microsatellite instability display mRNA expression signatures characteristic of increased immunogenicity

scientific article

Colorectal cancers with mononucleotide microsatellite instability can be identified using microfabricated chip technology

scientific article published in November 2003

Critical appraisal of quantitative PCR results in colorectal cancer research: can we rely on published qPCR results?

scientific article published on 02 January 2014

Cyclo-oxygenase-2 (COX-2) mRNA expression and hormone receptor status in breast cancer

scientific article published on 02 May 2006

Developments in real-time PCR research and molecular diagnostics ⬇️

scientific article published on 01 September 2010

Differential expression patterns of the insulin-like growth factor 2 gene in human colorectal cancer

scientific article published in January 2004

ERF-2, the human homologue of the murine Tis11d early response gene

scientific article

Elevated levels of RanBP7 mRNA in colorectal carcinoma are associated with increased proliferation and are similar to the transcription pattern of the proto-oncogene c-myc

scientific article published on 01 May 2000

Evidence for a link between IGF-I and cancer.

scientific article published in August 2004

Expression of 25-hydroxyvitamin D-1-alpha-hydroxylase mRNA in individuals with colorectal cancer

scientific article published on 01 May 2002

Expression of 25-hydroxyvitamin D-1-alpha-hydroxylase, and vitamin D receptor mRNA in normal and malignant breast tissue.

scientific article

Expression of HLA class II in colorectal cancer: evidence for enhanced immunogenicity of microsatellite-instability-positive tumours

scientific article

Expression of PRPF31 mRNA in patients with autosomal dominant retinitis pigmentosa: a molecular clue for incomplete penetrance?

scientific article (publication date: October 2003)

Expression of the Ca2+-activated chloride channel genes CLCA1 and CLCA2 is downregulated in human colorectal cancer

scientific article published on 01 June 2001

Extended silencing of the insulin-like growth factor-I receptor gene in MCF-7 cells by serial transfection with siRNA.

scientific article published on 8 August 2006

Five years MIQE guidelines: the case of the Arabian countries

scientific article

Gene expression profiling for molecular staging and prognosis prediction in colorectal cancer.

scientific article published in September 2004

Genetic detection of lymph node micrometastases in patients with colorectal cancer

scientific article published on 01 January 1998

Homogeneous and digital proximity ligation assays for the detection of Clostridium difficile toxins A and B.

scientific article

How to make Mathematics Biology's next and better microscope.

scientific article published on 10 September 2014

Immunogenic hsp-70 is overexpressed in colorectal cancers with high-degree microsatellite instability

scientific article published on 01 December 2005

Improving the analysis of quantitative PCR data in veterinary research

scientific article published on 17 October 2011

Improving the standardization of mRNA measurement by RT-qPCR.

scientific article

In silico tools for qPCR assay design and data analysis.

scientific article

Increased levels of insulin-like growth factor binding protein-2 in sera and tumours from patients with colonic neoplasia with and without acromegaly

scientific article published in April 2001

Influence of trypsinization and alternative procedures for cell preparation before RNA extraction on RNA integrity.

scientific article published on 28 June 2014

Insufficient demonstration of long-term stability of Aspergillus galactomannan

scientific article published in November 2014

Intracellular expression profiles measured by real-time PCR tomography in the Xenopus laevis oocyte

scientific article published on 26 November 2007

Lamina propria macrophage phenotypes in relation to Escherichia coli in Crohn's disease.

scientific article published on 3 July 2015

Local expression of insulin-like growth factor-I affects angiogenesis in colorectal cancer

scientific article published on 01 May 2002

MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments

scientific article published on 21 September 2010

Metasin-an intra-operative RT-qPCR assay to detect metastatic breast cancer in sentinel lymph nodes.

scientific article published on 24 June 2013

Microsatellite and chromosomal stable colorectal cancers demonstrate poor immunogenicity and early disease recurrence

scientific article published on 15 July 2008

Minimum information necessary for quantitative real-time PCR experiments.

scientific article published in January 2014

Molecular assessment of tumour stage and disease recurrence using PCR-based assays

scientific article published on 01 September 1998

Molecular medicine, gene-expression profiling and molecular diagnostics: putting the cart before the horse

scientific article published on 01 June 2008

Molecular quantification and mapping of lymph-node micrometastases in cervical cancer

scientific article published on 01 January 2001

Molecular staging of colorectal cancer: new paradigm or waste of time?

scientific article published in September 2007

Nuclear transcription factors: potential targets for new modes of intervention in skin disease.

scientific article published on November 1994

Nucleic acid quantification and disease outcome prediction in colorectal cancer

scientific article published on 01 May 2006

Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction

scientific article published in September 2004

Primer sequence disclosure: a clarification of the MIQE guidelines.

scientific article

Proximity assays for sensitive quantification of proteins

scientific article

Quantification of cytokeratin 20, carcinoembryonic antigen and guanylyl cyclase C mRNA levels in lymph nodes may not predict treatment failure in colorectal cancer patients

scientific article

Quantification of mRNA using real-time RT-PCR

scientific article

Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems.

scientific article

Quantitative real-time RT-PCR--a perspective.

scientific article

Quantitative real-time reverse transcription polymerase chain reaction: normalization to rRNA or single housekeeping genes is inappropriate for human tissue biopsies.

scientific article published in October 2002

RDML-Ninja and RDMLdb for standardized exchange of qPCR data

scientific article

RNA biomarkers in colorectal cancer.

scientific article

RT-qPCR Testing of SARS-CoV-2: A Primer

scientific article published on 24 April 2020

Real-time RT-PCR normalisation; strategies and considerations.

scientific article

Real-time quantitative PCR, pathogen detection and MIQE.

scientific article published in January 2013

Real-time, fluorescence-based quantitative PCR: a snapshot of current procedures and preferences

scientific article

Recent progress in developing proximity ligation assays for pathogen detection.

scientific article published on 08 May 2015

Reduced expression of the growth hormone and type 1 insulin-like growth factor receptors in human somatotroph tumours and an analysis of possible mutations of the growth hormone receptor.

scientific article published in September 2003

Reliability of real-time reverse-transcription PCR in clinical diagnostics: gold standard or substandard?

scientific article published on March 2009

SPUD: a quantitative PCR assay for the detection of inhibitors in nucleic acid preparations.

scientific article

Significant Decline in Galactomannan Signal during Storage of Clinical Serum Samples ⬇️

scientific article published on June 24, 2013

Talking the talk, but not walking the walk: RT-qPCR as a paradigm for the lack of reproducibility in molecular research

scientific article published on 10 August 2017

Tenth annual nucleic acid-based technologies: time to stop and think. Washington, DC, USA, 24-26 June, 2002.

scientific article published in September 2002

The GH-IGF-I axis and breast cancer.

scientific article

The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments

scientific article (publication date: April 2009)

The digital MIQE guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

scientific article

The expression of the growth hormone secretagogue receptor ligand ghrelin in normal and abnormal human pituitary and other neuroendocrine tumors

scientific article published in February 2001

The human immediate early gene BRF1 maps to chromosome 14q22-q24

scientific article

The immunogenicity of colorectal cancers with high-degree microsatellite instability

scientific article

The mRNA expression of cyclo-oxygenase-2 (COX-2) and vascular endothelial growth factor (VEGF) in human breast cancer

scientific article

The need for transparency and good practices in the qPCR literature.

scientific article published in November 2013

The product of the primary response gene BRF1 inhibits the interaction between 14-3-3 proteins and cRaf-1 in the yeast trihybrid system

scientific article published on 01 August 1999

The reproducibility of biomedical research: Sleepers awake!

scientific article published on December 2014

The role of apoptosis (programmed cell death) in haemopoiesis and the immune system.

scientific article

The tissue distribution of the mRNA of ghrelin and subtypes of its receptor, GHS-R, in humans

scientific article published in June 2002

The value of microarray techniques for quantitative gene profiling in molecular diagnostics

scientific article published on 01 June 2002

Transcription of the inositol polyphosphate 1-phosphatase gene (INPP1) is upregulated in human colorectal cancer

scientific article published on 01 April 2000

Transparency of Reporting in Molecular Diagnostics ⬇️

scientific article published on July 30, 2013

Tumour-infiltrating lymphocytes in colorectal cancer with microsatellite instability are activated and cytotoxic

scientific article published on 01 April 2004

Unreliable real-time PCR analysis of human endogenous retrovirus-W (HERV-W) RNA expression and DNA copy number in multiple sclerosis.

scientific article

Validation of housekeeping genes for normalizing RNA expression in real-time PCR.

scientific article

Variability of the reverse transcription step: practical implications.

scientific article published on 31 October 2014

Vitamin D metabolism in peripheral blood mononuclear cells is influenced by chewing "betel nut" (Areca catechu) and vitamin D status

scientific article published on 2 May 2006

Vitamin D receptor (VDR) mRNA and VDR protein levels in relation to vitamin D status, insulin secretory capacity, and VDR genotype in Bangladeshi Asians

scientific article published in July 2002

hTERT expression in human breast cancer and non-cancerous breast tissue: correlation with tumour stage and c-Myc expression.

scientific article

qPCR, dPCR, NGS - A journey.

scientific article published on 15 January 2015

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