List of works - Martina Pohl

(R,R)-Butane-2,3-diol dehydrogenase from Bacillus clausii DSM 8716T: Cloning and expression of the bdhA-gene, and initial characterization of enzyme

scientific article published on 06 August 2017

(S)-Selective MenD variants from Escherichia coli provide access to new functionalized chiral α-hydroxy ketones

scientific article published in March 2013

A FRET-based biosensor for the quantification of glucose in culture supernatants of mL scale microbial cultivations

scientific article published on 21 August 2019

A Synthetic Reaction Cascade Implemented by Colocalization of Two Proteins within Catalytically Active Inclusion Bodies

scientific article published on 20 August 2018

A Tailor-Made Chimeric Thiamine Diphosphate Dependent Enzyme for the Direct Asymmetric Synthesis of (S)-Benzoins

article

A Toolbox of Genetically Encoded FRET-Based Biosensors for Rapid l-Lysine Analysis

scientific article

A high-throughput screening assay for hydroxynitrile lyase activity

scientific article

A new perspective on thiamine catalysis

scientific article published in August 2004

A standard numbering scheme for thiamine diphosphate-dependent decarboxylases

scientific article

Advanced in vivo applications of blue light photoreceptors as alternative fluorescent proteins

scientific article

Advantages of Hydrogel-Based 3D-Printed Enzyme Reactors and Their Limitations for Biocatalysis

Alteration of the substrate specificity of benzoylformate decarboxylase from Pseudomonas putida by directed evolution

scientific article published in August 2003

An Efficient Route to Both Enantiomers of allo-Threonine by Simultaneous Amino Acid Racemase-Catalyzed Isomerization of Threonine and Crystallization

An Enzymatic 2-Step Cofactor and Co-Product Recycling Cascade towards a Chiral 1,2-Diol. Part I: Cascade Design.

scientific article published on 14 May 2019

An Orthogonal Biocatalytic Approach for the Safe Generation and Use of HCN in a Multistep Continuous Preparation of Chiral O-Acetylcyanohydrins

An R-selective hydroxynitrile lyase from Arabidopsis thaliana with an alpha/beta-hydrolase fold

scientific article published in January 2007

An activity, stability and selectivity comparison of propioin synthesis by thiamine diphosphate-dependent enzymes in a solid/gas bioreactor

scientific article published in June 2007

An evaluation of genetically encoded FRET-based biosensors for quantitative metabolite analyses in vivo

scientific article published on 5 August 2014

Application of α-keto acid decarboxylases in biotransformations ⬇️

scientific article published on June 29, 1998

Asymmetric Stetter reactions catalyzed by thiamine diphosphate-dependent enzymes

scientific article published on 24 June 2014

Asymmetric Synthesis of Aliphatic 2-Hydroxy Ketones by Enzymatic Carboligation of Aldehydes

Asymmetric synthesis of (S)-phenylacetylcarbinol – closing a gap in C–C bond formation

Asymmetric synthesis of chiral 2-hydroxy ketones by coupled biocatalytic alkene oxidation and CC bond formation

BioCatNet: A Database System for the Integration of Enzyme Sequences and Biocatalytic Experiments

scientific article

Biochemical characterization of an alcohol dehydrogenase fromRalstoniasp

scientific article published on 22 February 2013

Branched-Chain Keto Acid Decarboxylase fromLactococcus lactis (KdcA), a Valuable Thiamine Diphosphate-Dependent Enzyme for Asymmetric CC Bond Formation

CC bond formation using ThDP-dependent lyases

scientific article published on 20 March 2013

Catalytically active inclusion bodies of L-lysine decarboxylase from E. coli for 1,5-diaminopentane production

scientific article published on 11 April 2018

Catalytically-active inclusion bodies-Carrier-free protein immobilizates for application in biotechnology and biomedicine.

scientific article published on 29 April 2017

Characterization of benzaldehyde lyase from Pseudomonas fluorescens: A versatile enzyme for asymmetric C-C bond formation

scientific article published on 31 October 2006

Characterization of phenylpyruvate decarboxylase, involved in auxin production of Azospirillum brasilense

scientific article published on 31 August 2007

Closing the gap for efficient immobilization of biocatalysts in continuous processes: HaloTag™ fusion enzymes for a continuous enzymatic cascade towards a vicinal chiral diol

Comparative characterisation of thiamin diphosphate-dependent decarboxylases

Continuous enzymatic carboligation of benzaldehyde and acetaldehyde in an enzyme ultrafiltration membrane reactor and laminar flow microreactors

Conversion of Pyruvate Decarboxylase into an Enantioselective Carboligase with Biosynthetic Potential

scientific article published on 22 February 2011

Corrigendum: A Tailor-Made Chimeric Thiamine Diphosphate Dependent Enzyme for the Direct Asymmetric Synthesis of (S)-Benzoins

article

Development of a donor-acceptor concept for enzymatic cross-coupling reactions of aldehydes: the first asymmetric cross-benzoin condensation

scientific article published in October 2002

Effect of oxygen limitation and medium composition on Escherichia coli fermentation in shake-flask cultures

scientific article

Effective Production of (S)-α-Hydroxy ketones: An Reaction Engineering Approach

article

Efficient 2-step biocatalytic strategies for the synthesis of all nor(pseudo)ephedrine isomers

article

Ein maßgeschneidertes chimäres Thiamindiphosphat-abhängiges Enzym zur direkten asymmetrischen Synthese von (S)-Benzoinen

article

Encapsulation of FRET-based glucose and maltose biosensors to develop functionalized silica nanoparticles

scientific article published on 26 January 2016

Engineering stereoselectivity of ThDP-dependent enzymes

scientific article published on 13 September 2013

Entwicklung einer Enzymplattform für die biokatalytische C-C-Verknüpfung

Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putida.

scientific article published in June 2005

Expedient synthesis of C-aryl carbohydrates by consecutive biocatalytic benzoin and aldol reactions.

scientific article

Factors influencing the operational stability of NADPH-dependent alcohol dehydrogenase and an NADH-dependent variant thereof in gas/solid reactors

Factors mediating activity, selectivity, and substrate specificity for the thiamin diphosphate-dependent enzymes benzaldehyde lyase and benzoylformate decarboxylase.

scientific article published in December 2006

Fusion of a Coiled-Coil Domain Facilitates the High-Level Production of Catalytically Active Enzyme Inclusion Bodies

article

Fusion of a flavin-based fluorescent protein to hydroxynitrile lyase from Arabidopsis thaliana improves enzyme stability

scientific article published on 31 May 2013

Genetically Encoded Förster Resonance Energy Transfer-Based Biosensors Studied on the Single-Molecule Level

scientific article published on 18 July 2018

HaloTag™: Evaluation of a covalent one-step immobilization for biocatalysis

scientific article published on 3 December 2016

High-throughput assay of ( R)-phenylacetylcarbinol synthesized by pyruvate decarboxylase

scientific article published on 19 October 2002

Hydroxynitrile Lyase from Arabidopsis thaliana: Identification of Reaction Parameters for Enantiopure Cyanohydrin Synthesis by Pure and Immobilized Catalyst

Hydroxynitrile Lyases with α/β-Hydrolase Fold: Two Enzymes with Almost Identical 3D Structures but Opposite Enantioselectivities and Different Reaction Mechanisms

scientific article

Hydroxynitrile lyase catalyzed cyanohydrin synthesis at high pH-values

scientific article

Identification of novel benzoylformate decarboxylases by growth selection

scientific article

Impact of Molecular Crowding on Translational Mobility and Conformational Properties of Biological Macromolecules

scientific article published on 15 May 2019

Improved biocatalysts by directed evolution and rational protein design

scientific article (publication date: April 2001)

Influence of Organic Solvents on Enzymatic Asymmetric Carboligations

scientific article published on 4 October 2012

Influence of the hydrostatic pressure and pH on the asymmetric 2-hydroxyketone formation catalyzed by Pseudomonas putida benzoylformate decarboxylase and variants thereof

scientific article published in May 2010

Mechanism of acetaldehyde-induced deactivation of microbial lipases

scientific article

MenD from Bacillus subtilis : A Potent Catalyst for the Enantiocomplementary Asymmetric Synthesis of Functionalized α-Hydroxy Ketones

article

Navigating within thiamine diphosphate-dependent decarboxylases: Sequences, structures, functional positions, and binding sites

scientific article published on 20 May 2019

Neue Biokatalysatoren für die Weiße Biotechnologie

Novel biocatalysts for white biotechnology

scientific article published on 01 July 2006

Phenylalanine ammonia lyase from Arabidopsis thaliana (AtPAL2): A potent MIO-enzyme for the synthesis of non-canonical aromatic alpha-amino acids.: Part II: Application in different reactor concepts for the production of (S)-2-chloro-phenylalanine.

scientific article published in May 2017

Phenylalanine ammonia lyase from Arabidopsis thaliana (AtPAL2): A potent MIO-enzyme for the synthesis of non-canonical aromatic alpha-amino acids: Part I: Comparative characterization to the enzymes from Petroselinum crispum (PcPAL1) and Rhodosporid

scientific article published on 6 April 2017

Physiological relation between respiration activity and heterologous expression of selected benzoylformate decarboxylase variants in Escherichia coli

scientific article published on 19 October 2010

Preparative enantioselective synthesis of benzoins and (R)-2-hydroxy-1-phenylpropanone using benzaldehyde lyase

Propioin synthesis using thiamine diphosphate-dependent enzymes

scientific article published in January 2009

Purification and simultaneous immobilization of Arabidopsis thaliana hydroxynitrile lyase using a family 2 carbohydrate-binding module

scientific article published on 13 April 2015

Rapid, selective and stable HaloTag-LbADH immobilization directly from crude cell extract for the continuous biocatalytic production of chiral alcohols and epoxides

article

Rational protein design of ThDP-dependent enzymes-engineering stereoselectivity

scientific article

Regio- and Stereoselective Aliphatic-Aromatic Cross-Benzoin Reaction: Enzymatic Divergent Catalysis

scientific article published on 12 August 2016

S-Selective Mixed Carboligation by Structure-Based Design of the Pyruvate Decarboxylase from Acetobacter pasteurianus

Single-Molecule Studies on a FRET Biosensor: Lessons from a Comparison of Fluorescent Protein Equipped versus Dye-Labeled Species

scientific article published on 27 November 2018

Stereoselective Reduction of 2-Hydroxy Ketones towards syn- and anti-1,2-Diols

article published in 2011

Stereoselective synthesis of bulky 1,2-diols with alcohol dehydrogenases

article

Structural and Mutagenesis Studies of the Thiamine-Dependent, Ketone-Accepting YerE from Pseudomonas protegens

scientific article published on 11 October 2018

Structure elucidation of the thermal degradation products of the nucleotide cofactors NADH and NADPH by nano-ESI-FTICR-MS and HPLC-MS.

scientific article published on 30 August 2010

Structure of the branched-chain keto acid decarboxylase (KdcA) from Lactococcus lactis provides insights into the structural basis for the chemoselective and enantioselective carboligation reaction

scientific article

Substrate and water adsorption phenomena in a gas/solid enzymatic reactor.

scientific article published in May 2009

Synthesis of chiral cyanohydrins by recombinant Escherichia coli cells in a micro-aqueous reaction system

scientific article published on 27 April 2012

Synthesis of α-hydroxy ketones and vicinal diols with the Bacillus licheniformis DSM 13T butane-2,3-diol dehydrogenase

scientific article published on 22 September 2020

Synthetic peptides as antagonists of the anaphylatoxin C3a

scientific article published on November 15, 1992

TTC-based screening assay for ω-transaminases: a rapid method to detect reduction of 2-hydroxy ketones

scientific article published on 2 January 2012

Tailor-made catalytically active inclusion bodies for different applications in biocatalysis

Tailoring a Stabilized Variant of Hydroxynitrile Lyase from Arabidopsis thaliana

scientific article published on 29 February 2012

Tailoring theS-Selectivity of 2-Succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate Synthase (MenD) fromEscherichia coli

article

The Enzymatic Asymmetric Conjugate Umpolung Reaction

The enzymatic asymmetric conjugate umpolung reaction

scientific article published on September 2010

The production of (R)-2-hydroxy-1-phenyl-propan-1-one derivatives by benzaldehyde lyase from Pseudomonas fluorescens in a continuously operated membrane reactor

scientific article published in April 2007

Thiamin diphosphate in biological chemistry: exploitation of diverse thiamin diphosphate-dependent enzymes for asymmetric chemoenzymatic synthesis.

scientific article

Thiamin-diphosphate-dependent enzymes: new aspects of asymmetric C-C bond formation

scientific article

Towards a Mechanistic Understanding of Factors Controlling the Stereoselectivity of Transketolase

Two steps in one pot: enzyme cascade for the synthesis of nor(pseudo)ephedrine from inexpensive starting materials

scientific article published on 9 May 2013

Uneven twins: comparison of two enantiocomplementary hydroxynitrile lyases with alpha/beta-hydrolase fold.

scientific article published on 27 March 2009

μMORE: A microfluidic magnetic oscillation reactor for accelerated parameter optimization in biocatalysis

scientific article published on 8 June 2016

List of works by Martina Pohl

(R,R)-Butane-2,3-diol dehydrogenase from Bacillus clausii DSM 8716T: Cloning and expression of the bdhA-gene, and initial characterization of enzyme

(S)-Selective MenD variants from Escherichia coli provide access to new functionalized chiral α-hydroxy ketones

A FRET-based biosensor for the quantification of glucose in culture supernatants of mL scale microbial cultivations

A Synthetic Reaction Cascade Implemented by Colocalization of Two Proteins within Catalytically Active Inclusion Bodies

A Tailor-Made Chimeric Thiamine Diphosphate Dependent Enzyme for the Direct Asymmetric Synthesis of (S)-Benzoins

A Toolbox of Genetically Encoded FRET-Based Biosensors for Rapid l-Lysine Analysis

A high-throughput screening assay for hydroxynitrile lyase activity

A new perspective on thiamine catalysis

A standard numbering scheme for thiamine diphosphate-dependent decarboxylases

Advanced in vivo applications of blue light photoreceptors as alternative fluorescent proteins

Advantages of Hydrogel-Based 3D-Printed Enzyme Reactors and Their Limitations for Biocatalysis

Alteration of the substrate specificity of benzoylformate decarboxylase from Pseudomonas putida by directed evolution

An Efficient Route to Both Enantiomers of allo-Threonine by Simultaneous Amino Acid Racemase-Catalyzed Isomerization of Threonine and Crystallization

An Enzymatic 2-Step Cofactor and Co-Product Recycling Cascade towards a Chiral 1,2-Diol. Part I: Cascade Design.

An Orthogonal Biocatalytic Approach for the Safe Generation and Use of HCN in a Multistep Continuous Preparation of Chiral O-Acetylcyanohydrins

An R-selective hydroxynitrile lyase from Arabidopsis thaliana with an alpha/beta-hydrolase fold

An activity, stability and selectivity comparison of propioin synthesis by thiamine diphosphate-dependent enzymes in a solid/gas bioreactor

An evaluation of genetically encoded FRET-based biosensors for quantitative metabolite analyses in vivo

Application of α-keto acid decarboxylases in biotransformations ⬇️

Asymmetric Stetter reactions catalyzed by thiamine diphosphate-dependent enzymes

Asymmetric Synthesis of Aliphatic 2-Hydroxy Ketones by Enzymatic Carboligation of Aldehydes

Asymmetric synthesis of (S)-phenylacetylcarbinol – closing a gap in C–C bond formation

Asymmetric synthesis of chiral 2-hydroxy ketones by coupled biocatalytic alkene oxidation and CC bond formation

BioCatNet: A Database System for the Integration of Enzyme Sequences and Biocatalytic Experiments

Biochemical characterization of an alcohol dehydrogenase fromRalstoniasp

Branched-Chain Keto Acid Decarboxylase fromLactococcus lactis (KdcA), a Valuable Thiamine Diphosphate-Dependent Enzyme for Asymmetric CC Bond Formation

CC bond formation using ThDP-dependent lyases

Catalytically active inclusion bodies of L-lysine decarboxylase from E. coli for 1,5-diaminopentane production

Catalytically-active inclusion bodies-Carrier-free protein immobilizates for application in biotechnology and biomedicine.

Characterization of benzaldehyde lyase from Pseudomonas fluorescens: A versatile enzyme for asymmetric C-C bond formation

Characterization of phenylpyruvate decarboxylase, involved in auxin production of Azospirillum brasilense

Closing the gap for efficient immobilization of biocatalysts in continuous processes: HaloTag™ fusion enzymes for a continuous enzymatic cascade towards a vicinal chiral diol

Comparative characterisation of thiamin diphosphate-dependent decarboxylases

Continuous enzymatic carboligation of benzaldehyde and acetaldehyde in an enzyme ultrafiltration membrane reactor and laminar flow microreactors

Conversion of Pyruvate Decarboxylase into an Enantioselective Carboligase with Biosynthetic Potential

Corrigendum: A Tailor-Made Chimeric Thiamine Diphosphate Dependent Enzyme for the Direct Asymmetric Synthesis of (S)-Benzoins

Development of a donor-acceptor concept for enzymatic cross-coupling reactions of aldehydes: the first asymmetric cross-benzoin condensation

Effect of oxygen limitation and medium composition on Escherichia coli fermentation in shake-flask cultures

Effective Production of (S)-α-Hydroxy ketones: An Reaction Engineering Approach

Efficient 2-step biocatalytic strategies for the synthesis of all nor(pseudo)ephedrine isomers

Ein maßgeschneidertes chimäres Thiamindiphosphat-abhängiges Enzym zur direkten asymmetrischen Synthese von (S)-Benzoinen

Encapsulation of FRET-based glucose and maltose biosensors to develop functionalized silica nanoparticles

Engineering stereoselectivity of ThDP-dependent enzymes

Entwicklung einer Enzymplattform für die biokatalytische C-C-Verknüpfung

Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putida.

Expedient synthesis of C-aryl carbohydrates by consecutive biocatalytic benzoin and aldol reactions.

Factors influencing the operational stability of NADPH-dependent alcohol dehydrogenase and an NADH-dependent variant thereof in gas/solid reactors

Factors mediating activity, selectivity, and substrate specificity for the thiamin diphosphate-dependent enzymes benzaldehyde lyase and benzoylformate decarboxylase.

Fusion of a Coiled-Coil Domain Facilitates the High-Level Production of Catalytically Active Enzyme Inclusion Bodies

Fusion of a flavin-based fluorescent protein to hydroxynitrile lyase from Arabidopsis thaliana improves enzyme stability

Genetically Encoded Förster Resonance Energy Transfer-Based Biosensors Studied on the Single-Molecule Level

HaloTag™: Evaluation of a covalent one-step immobilization for biocatalysis

High-throughput assay of ( R)-phenylacetylcarbinol synthesized by pyruvate decarboxylase

Hydroxynitrile Lyase from Arabidopsis thaliana: Identification of Reaction Parameters for Enantiopure Cyanohydrin Synthesis by Pure and Immobilized Catalyst

Hydroxynitrile Lyases with α/β-Hydrolase Fold: Two Enzymes with Almost Identical 3D Structures but Opposite Enantioselectivities and Different Reaction Mechanisms

Hydroxynitrile lyase catalyzed cyanohydrin synthesis at high pH-values

Identification of novel benzoylformate decarboxylases by growth selection

Impact of Molecular Crowding on Translational Mobility and Conformational Properties of Biological Macromolecules

Improved biocatalysts by directed evolution and rational protein design

Influence of Organic Solvents on Enzymatic Asymmetric Carboligations

Influence of the hydrostatic pressure and pH on the asymmetric 2-hydroxyketone formation catalyzed by Pseudomonas putida benzoylformate decarboxylase and variants thereof

Mechanism of acetaldehyde-induced deactivation of microbial lipases

MenD from Bacillus subtilis : A Potent Catalyst for the Enantiocomplementary Asymmetric Synthesis of Functionalized α-Hydroxy Ketones

Navigating within thiamine diphosphate-dependent decarboxylases: Sequences, structures, functional positions, and binding sites

Neue Biokatalysatoren für die Weiße Biotechnologie

Novel biocatalysts for white biotechnology

Phenylalanine ammonia lyase from Arabidopsis thaliana (AtPAL2): A potent MIO-enzyme for the synthesis of non-canonical aromatic alpha-amino acids.: Part II: Application in different reactor concepts for the production of (S)-2-chloro-phenylalanine.

Phenylalanine ammonia lyase from Arabidopsis thaliana (AtPAL2): A potent MIO-enzyme for the synthesis of non-canonical aromatic alpha-amino acids: Part I: Comparative characterization to the enzymes from Petroselinum crispum (PcPAL1) and Rhodosporid

Physiological relation between respiration activity and heterologous expression of selected benzoylformate decarboxylase variants in Escherichia coli

Preparative enantioselective synthesis of benzoins and (R)-2-hydroxy-1-phenylpropanone using benzaldehyde lyase

Propioin synthesis using thiamine diphosphate-dependent enzymes

Purification and simultaneous immobilization of Arabidopsis thaliana hydroxynitrile lyase using a family 2 carbohydrate-binding module

Rapid, selective and stable HaloTag-LbADH immobilization directly from crude cell extract for the continuous biocatalytic production of chiral alcohols and epoxides

Rational protein design of ThDP-dependent enzymes-engineering stereoselectivity

Regio- and Stereoselective Aliphatic-Aromatic Cross-Benzoin Reaction: Enzymatic Divergent Catalysis

S-Selective Mixed Carboligation by Structure-Based Design of the Pyruvate Decarboxylase from Acetobacter pasteurianus

Single-Molecule Studies on a FRET Biosensor: Lessons from a Comparison of Fluorescent Protein Equipped versus Dye-Labeled Species

Stereoselective Reduction of 2-Hydroxy Ketones towards syn- and anti-1,2-Diols

Stereoselective synthesis of bulky 1,2-diols with alcohol dehydrogenases