List of works by Wolfgang Hillen

A bidirectionally active signal for termination of transcription is located between tetA and orfL on transposon Tn10.

scientific article

A chimeric transactivator allows tetracycline-responsive gene expression in whole plants.

scientific article published in April 1994

A gene regulation system with four distinct expression levels

scientific article published on August 2006

A new strategy for the analysis of tetracycline residues in foodstuffs by a surface plasmon resonance biosensor

scientific article published on 10 June 2006

A novel method to analyze nucleocytoplasmic transport in vivo by using short peptide tags

scientific article published on 13 February 2013

A novel mode of control of Mycoplasma pneumoniae HPr kinase/phosphatase activity reflects its parasitic lifestyle

scientific article published in October 2002

A novel tetracycline-controlled transactivator-transrepressor system enables external control of oncolytic adenovirus replication

scientific article published in September 2003

A peptide triggers allostery in tet repressor by binding to a unique site

scientific article

A protein functional leap: how a single mutation reverses the function of the transcription regulator TetR

scientific article

A short autonomous repression motif is located within the N-terminal domain of CTCF.

scientific article

A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo

scientific article

A threonine to alanine exchange at position 40 of Tet repressor alters the recognition of the sixth base pair of tet operator from GC to AT.

scientific article published on December 1988

Acinetobacter calcoaceticus encoded mutarotase: nucleotide sequence analysis of the gene and characterization of its secretion in Escherichia coli

scientific article published on May 1986

Activity reversal of Tet repressor caused by single amino acid exchanges

scientific article published in August 2004

Adjusting transgene expression levels in lymphocytes with a set of inducible promoters

scientific article

Affinities of mAbs to Tet repressor complexed with operator or tetracycline suggest conformational changes associated with induction.

scientific article

Amino acids determining operator binding specificity in the helix-turn-helix motif of Tn10 Tet repressor ⬇️

scientific article published on December 1, 1991

An RNA aptamer that induces transcription

scientific article

An accurate method for determining the helical repeat of DNA in solution reveals differences to the crystal structures of two B-DNA decamers

scientific article published in November 1992

An exclusive α/β code directs allostery in TetR-peptide complexes

scientific article

An optimized conditional suicide switch using doxycycline-dependent expression of human tBid

scientific article

Analysis of tet operator-TET repressor complexes by thermal denaturation studies.

scientific article published on October 1982

Anhydrotetracycline-peptide conjugates as representatives for ligand-based transactivating systems

scientific article published on 22 June 2010

Backbone dynamics of Tet repressor alpha8intersectionalpha9 loop.

scientific article

Carbon source control of the phosphorylation state of the Bacillus subtilis carbon-flux regulator Crh in vivo.

scientific article

CcpA forms complexes with CodY and RpoA in Bacillus subtilis

scientific journal article

CcpA mutants with differential activities in Bacillus subtilis

scientific article published on 01 January 2007

Characterization of non-inducible Tet repressor mutants suggests conformational changes necessary for induction

scientific article published on 01 August 1995

Click-chemistry-derived tetracycline-amino acid conjugates exhibiting exceptional potency and exclusive recognition of the reverse tet repressor

scientific article

Comparison of tetracycline and tigecycline binding to ribosomes mapped by dimethylsulphate and drug-directed Fe2+ cleavage of 16S rRNA.

scientific article published on 25 February 2004

Complementation of a Delta ccpA mutant of Lactobacillus casei with CcpA mutants affected in the DNA- and cofactor-binding domains.

scientific article published in March 2004

Complex formation between malate dehydrogenase and isocitrate dehydrogenase from Bacillus subtilis is regulated by tricarboxylic acid cycle metabolites

scientific journal article

Computer simulations and experimental studies of gel mobility patterns for weak and strong non-cooperative protein binding to two targets on the same DNA: application to binding of tet repressor variants to multiple and single tet operator sites

scientific article published on March 1991

Conditional cell suicide using dox‐dependent caspase‐2 expression ⬇️

scientific article published on April 1, 2003

Conditional gene expression by controlling translation with tetracycline-binding aptamers

scientific article

Conformational changes induced in the Tet repressor protein TetR(B) upon operator or anhydrotetracycline binding as revealed by time-resolved fluorescence spectroscopy on single tryptophan mutants.

scientific article

Conformational changes necessary for gene regulation by Tet repressor assayed by reversible disulfide bond formation

scientific article

Conformational changes of the Tet repressor induced by tetracycline trapping

scientific article

Constitutive expression of tetracycline resistance mediated by a Tn10-like element in Haemophilus parainfluenzae results from a mutation in the repressor gene

scientific article

Contacts between Tet repressor and tet operator revealed by new recognition specificities of single amino acid replacement mutants

scientific article published on August 20, 1992

Control of expression of the Tn10-encoded tetracycline resistance genes. Equilibrium and kinetic investigation of the regulatory reactions.

scientific article

Control of expression of the Tn10-encoded tetracycline resistance operon II. Interaction of RNA polymerase and TET repressor with the tet operon regulatory region ⬇️

scientific article published on January 15, 1984

Correlation of thermodynamic and genetic properties in the Tn10 encoded TET gene control region.

scientific article published on April 1982

Coupling physiology and gene regulation in bacteria: the phosphotransferase sugar uptake system delivers the signals

scientific article published on 01 December 1998

Cross communication between components of carbon catabolite repression of Lactobacillus casei and Bacillus megaterium.

scientific article published in September 2002

Crystal structure of HPr kinase/phosphatase from Mycoplasma pneumoniae

scientific article (publication date: 28 February 2003)

Crystal structure of the tet repressor in complex with a novel tetracycline, 9-(N,N-dimethylglycylamido)- 6-demethyl-6-deoxy-tetracycline

scientific article

Crystallization and preliminary X-ray analyses of catabolite control protein A, free and in complex with its DNA-binding site

scientific article published on 01 January 2000

Crystallization of and preliminary X-ray diffraction data for TET-repressor and the TET-repressor-tetracycline complex.

scientific article published on December 1984

Crystallization, preliminary X-ray analysis and biophysical characterization of HPr kinase/phosphatase of Mycoplasma pneumoniae

scientific article published on 21 February 2002

Deletion mutagenesis of Tn 10 Tet repressor--localization of regions important for dimerization and inducibility in vivo.

scientific article

Determinants of protein-protein recognition by four helix bundles: changing the dimerization specificity of Tet repressor

scientific article published on January 1998

Determination of the equilibrium association constant between tet repressor and tetracycline at limiting Mg2+ concentrations: A generally applicable method for effector-dependent high-affinity complexes ⬇️

scientific article published on 01 December 1991

Development of a transactivator in hepatoma cells that allows expression of phase I, phase II, and chemical defense genes.

scientific article published on 31 August 2005

Diarylpropane-1,3-dione derivatives as TetR-inducing tetracycline mimetics: Synthesis and biological investigations

scientific article published in December 2009

Differential regulation of the Tn10-encoded tetracycline resistance genes tetA and tetR by the tandem tet operators O1 and O2.

scientific article published on February 1988

Domain motions accompanying Tet repressor induction defined by changes of interspin distances at selectively labeled sites

scientific article published on 01 July 1999

Doxycycline-regulated lentiviral vector system with a novel reverse transactivator rtTA2S-M2 shows a tight control of gene expression in vitro and in vivo

scientific article published in March 2003

Dynamics of repressor-operator recognition: the Tn10-encoded tetracycline resistance control.

scientific article published in February 1988

Efficient and exclusive induction of Tet repressor by the oligopeptide Tip results from co-variation of their interaction site

scientific article published on June 2007

Electrophoretic analysis of protein-induced DNA bending and twist changes.

scientific article

Engineered Tet repressor mutants with single tryptophan residues as fluorescent probes. Solvent accessibilities of DNA and inducer binding sites and interaction with tetracycline

scientific article published on October 1987

Engineered Tet repressors with recognition specificity for the tetO-4C5G operator variant

scientific article published on 12 September 2007

Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity

scientific article

Expression of alkane hydroxylase from Acinetobacter sp. Strain ADP1 is induced by a broad range of n-alkanes and requires the transcriptional activator AlkR.

scientific article published on November 1998

Expression, inducer spectrum, domain structure, and function of MopR, the regulator of phenol degradation in Acinetobacter calcoaceticus NCIB8250.

scientific article published on February 1997

Fast large-scale purification of tetracycline repressor variants from overproducing Escherichia coli strains

scientific article

Functional roles of amino acid residues involved in forming the alpha-helix-turn-alpha-helix operator DNA binding motif of Tet repressor from Tn10

scientific article published on October 1, 1992

Functionally important residues of the Tet repressor inducing peptide TIP determined by a complete mutational analysis

scientific article published on 12 July 2008

Gene regulation by tetracyclines

scientific article published in January 2004

Gene regulation by tetracyclines. Constraints of resistance regulation in bacteria shape TetR for application in eukaryotes

scientific article

Generating tetracycline-inducible auxotrophy in Escherichia coli and Salmonella enterica serovar Typhimurium by using an insertion element and a hyperactive transposase

scientific article published on July 2006

Generation of conditional mutants in higher eukaryotes by switching between the expression of two genes

scientific article

Genetic organization, nucleotide sequence and regulation of expression of genes encoding phenol hydroxylase and catechol 1,2-dioxygenase in Acinetobacter calcoaceticus NCIB8250.

scientific article published on October 1995

HPr kinase/phosphatase of Bacillus subtilis: expression of the gene and effects of mutations on enzyme activity, growth and carbon catabolite repression

scientific article published on 01 June 2002

Heterologous repressor-operator recognition among four classes of tetracycline resistance determinants

scientific article

How an agonist peptide mimics the antibiotic tetracycline to induce Tet-repressor

scientific article

How does Mg(2+) affect the binding of anhydrotetracycline in the TetR protein?

scientific article published in January 2004

Identification and characterization of xcpR encoding a subunit of the general secretory pathway necessary for dodecane degradation in Acinetobacter calcoaceticus ADP1 ⬇️

scientific article published on July 1, 1997

Identification of a stability determinant on the edge of the Tet repressor four-helix bundle dimerization motif

scientific article published in March 2001

Improved single-chain transactivators of the Tet-On gene expression system

scientific article

In vivo activation of tetracycline repressor by Cre/lox-mediated gene assembly

scientific article published on 21 July 2009

In vivo gene silencing identifies the Mycobacterium tuberculosis proteasome as essential for the bacteria to persist in mice

scientific journal article

Independent regulation of two genes in Escherichia coli by tetracyclines and Tet repressor variants

scientific article

Inducible DNA-loop formation blocks transcriptional activation by an SV40 enhancer

scientific article

Induction of single chain tetracycline repressor requires the binding of two inducers

scientific article published on 9 August 2006

Integrative elements for Bacillus subtilis yielding tetracycline-dependent growth phenotypes

scientific article

Interference of components of the phosphoenolpyruvate phosphotransferase system with the central virulence gene regulator PrfA of Listeria monocytogenes

scientific article

Intragenic suppressors of induction-deficient TetR mutants: localization and potential mechanism of action

scientific article published on February 1998

Kinetic and equilibrium characterization of the Tet repressor-tetracycline complex by fluorescence measurements. Evidence for divalent metal ion requirement and energy transfer

scientific article published on 01 February 1986

Lack of a 5' non-coding region in Tn1721encodedtetRmRNA is associated with a low efficiency of translation and a short half-life inEscherichia coil ⬇️

scientific article published on September 11, 1991

Ligand-modulated binding of a gene regulatory protein to DNA. Quantitative analysis of cyclic-AMP induced binding of CRP from Escherichia coli to non-specific and specific DNA targets

scientific article published on 01 June 1989

Mechanism of Tet repressor induction by tetracyclines: length compensates for sequence in the alpha8-alpha9 loop

scientific article

Mechanisms underlying expression of Tn10 encoded tetracycline resistance

scientific article published on January 1994

Molecular mechanics analysis of Tet repressor TRP-43 fluorescence

scientific article published on April 1997

Mycoplasma pneumoniae HPr kinase/phosphorylase

scientific article

Nomenclature for new tetracycline resistance determinants

scientific article

Nomenclature for tetracycline resistance determinants

scientific article

Noninducible Tet repressor mutations map from the operator binding motif to the C terminus

scientific article

Nucleotide sequence of a putative periplasmic Mn superoxide dismutase from Acinetobacter calcoaceticus ADP1.

scientific article

Nucleotide sequence of the Tn10 encoded tetracycline resistance gene ⬇️

scientific article published on January 25, 1983

Nucleotide sequence of the gene, protein purification and characterization of the pSC101-encoded tetracycline resistance-gene-repressor ⬇️

scientific article published on November 1, 1984

Nucleotide sequence of the repressor gene of the RA1 tetracycline resistance determinant: structural and functional comparison with three related Tet repressor genes ⬇️

scientific article published on October 25, 1984

Observing conformational and activity changes of tet repressor in vivo.

scientific article

Oligo[d(C).(G)] runs exhibit a helical repeat of 11.1 bp in solution and cause slight DNA curvature when properly phased

scientific article published on May 1994

On the origin of selectivity in recognition by cyclic adenosine 3',5'-monophosphate receptor protein of its specific binding site of the lactose promoter region

scientific article published on 01 July 1983

Permeation of tetracyclines through membranes of liposomes and Escherichia coli

scientific article published on 01 January 2000

Phenotypes of combined tet repressor mutants for effector and operator recognition and allostery.

scientific article

Phosphoprotein Crh-Ser46-P displays altered binding to CcpA to effect carbon catabolite regulation

scientific article published on 29 November 2005

Pressure effect on the structure of the Tet repressor protein TetR(B).

scientific article published in November 2002

Promoter strength driving TetR determines the regulatory properties of Tet-controlled expression systems

scientific article

Protein expression can be monitored in yeast by peptide-mediated induction of TetR-controlled gene expression

scientific article published on 20 June 2012

Proximity mapping of the Tet repressor-tetracycline-Fe2+ complex by hydrogen peroxide mediated protein cleavage.

scientific article published in January 1995

Proximity probing of Tet repressor to tet operator by dimethylsulfate reveals protected and accessible functions for each recognized base-pair in the major groove.

scientific article published in February 1995

Quantification of the influence of HPrSer46P on CcpA-cre interaction

scientific article published in May 2002

Quantitative analysis of gene expression with an improved green fluorescent protein

article

Quantitative interdependence of coeffectors, CcpA and cre in carbon catabolite regulation of Bacillus subtilis

scientific article published in May 2005

RNA polymerase III catalysed transcription can be regulated in Saccharomyces cerevisiae by the bacterial tetracycline repressor-operator system

scientific article published on April 1992

Random insertion of a TetR-inducing peptide tag into Escherichia coli proteins allows analysis of protein levels by induction of reporter gene expression

scientific article published on August 2006

Regulated and prolonged expression of mIFN(alpha) in immunocompetent mice mediated by a helper-dependent adenovirus vector

scientific article published on December 2001

Regulated expression of HPrK/P does not affect carbon catabolite repression of the xyn operon and of rocG in Bacillus subtilis

scientific article published on 01 June 2006

Regulation of carbon catabolism in Bacillus species.

scientific article

Reproducible doxycycline-inducible transgene expression at specific loci generated by Cre-recombinase mediated cassette exchange

scientific article

RpoN (sigma 54) is required for conversion of phenol to catechol in Acinetobacter calcoaceticus

scientific article

Saturation mutagenesis of the Tn10-encoded tet operator O1. Identification of base-pairs involved in Tet repressor recognition

scientific article published on 01 August 1988

Short peptides act as inducers, anti-inducers and corepressors of Tet repressor

scientific article

Silencing Mycobacterium smegmatis by using tetracycline repressors

scientific article published on 4 May 2007

Single-chain Tet transregulators

scientific article

Solvent-exposed residues in the Tet repressor (TetR) four-helix bundle contribute to subunit recognition and dimer stability.

scientific article

Species-specific differences in the activity of PrfA, the key regulator of listerial virulence genes

scientific article

Specific binding of divalent metal ions to tetracycline and to the Tet repressor/tetracycline complex

scientific article

Spectroscopic investigation of Tet repressor tryptophan-43 upon specific and nonspecific DNA binding.

scientific article

Stepwise selection of TetR variants recognizing tet operator 4C with high affinity and specificity

scientific article published on 01 February 1998

Stepwise selection of TetR variants recognizing tet operator 6C with high affinity and specificity

scientific article published on 01 February 1998

Stringent doxycycline-dependent control of gene activities using an episomal one-vector system

scientific article

Structural Investigation of Tet Repressor Loop 154–167: A Time-Resolved Fluorescence Study of Three Single Trp Mutants

scientific article published on 01 October 1997

Structural analysis of the operator binding domain of Tn10-encoded Tet repressor: a time-resolved fluorescence and anisotropy study ⬇️

scientific article published on February 25, 1992

Structural basis for allosteric control of the transcription regulator CcpA by the phosphoprotein HPr-Ser46-P.

scientific article

Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system

scientific article

Structural mechanism for the fine-tuning of CcpA function by the small molecule effectors glucose 6-phosphate and fructose 1,6-bisphosphate

scientific article

Structural origins for selectivity and specificity in an engineered bacterial repressor-inducer pair

scientific article

Structural requirements of tetracycline-Tet repressor interaction: determination of equilibrium binding constants for tetracycline analogs with the Tet repressor

scientific article published on August 1991

Structure of the Tet repressor-tetracycline complex and regulation of antibiotic resistance

scientific article

Structure-based design of Tet repressor to optimize a new inducer specificity

scientific article

Structures of carbon catabolite protein A–(HPr-Ser46-P) bound to diverse catabolite response element sites reveal the basis for high-affinity binding to degenerate DNA operators ⬇️

scientific article published on November 23, 2010

Subtype Selective Tetracycline Agonists and their Application for a Two-Stage Regulatory System

scientific article published on 01 September 2006

TET repressor.tet operator complex formation induces conformational changes in the tet operator DNA.

scientific article published on February 1984

Teaching TetR to recognize a new inducer

scientific article

Tet repressor binding induced curvature of tet operator DNA

scientific article published on August 1989

Tet repressor induction without Mg2+

scientific article published on 01 September 2000

Tet repressor mutants with altered effector binding and allostery

scientific article published in September 2005

Tet repressor residues indirectly recognizing anhydrotetracycline

scientific article published on June 2004

Tetracycline analogs affecting binding to Tn10-Encoded Tet repressor trigger the same mechanism of induction

scientific article published on 01 June 1996

Tetracycline derivatives: alternative effectors for Tet transregulators

scientific article

Tetracycline repressor-regulated gene repression in recombinant human cytomegalovirus

scientific article published in April 1995

Tetracycline sensing using novel doxycycline derivatives immobilized on different surface plasmon resonance biosensor surfaces

scientific article published on February 2010

Tetracycline-dependent conditional gene knockout in Bacillus subtilis

scientific article published on February 2005

Tetracycline-dependent gene regulation: combinations of transregulators yield a variety of expression windows

scientific article

Tetracycline-inducible expression systems with reduced basal activity in mammalian cells

scientific article published on January 1999

Tetracycline-inducible systems for Drosophila

scientific article

Tetracyclines: antibiotic action, uptake, and resistance mechanisms

scientific article published on June 1996

The Tetracycline Repressor-A Paradigm for a Biological Switch

scientific article published in June 2000

The Tn10-encoded tetracycline resistance mRNA contains a translational silencer in the 5' nontranslated region ⬇️

scientific article published on April 1, 1992

The complex formed between Tet repressor and tetracycline-Mg2+ reveals mechanism of antibiotic resistance

scientific article

The p65 domain from NF-kappaB is an efficient human activator in the tetracycline-regulatable gene expression system

scientific article published on April 2000

The role of the variable region in Tet repressor for inducibility by tetracycline

scientific article published on 01 March 1997

Thermal denaturation of engineered tet repressor proteins and their complexes with tet operator and tetracycline studied by temperature gradient gel electrophoresis.

scientific article published in December 1988

Thermodynamic analysis of tetracycline-mediated induction of Tet repressor by a quantitative methylation protection assay

scientific article published on 01 December 1995

Tight control of gene expression by a helper-dependent adenovirus vector carrying the rtTA2(s)-M2 tetracycline transactivator and repressor system.

scientific article published in November 2002

Transactivator mutants with altered effector specificity allow selective regulation of two genes by tetracycline variants.

scientific article published in April 2004

Transcriptional activation by tetracyclines in mammalian cells

scientific article

Trp scanning analysis of Tet repressor reveals conformational changes associated with operator and anhydrotetracycline binding

scientific article published on February 2000

Tryptophan in alpha-helix 3 of Tet repressor forms a sequence-specific contact with tet operator in solution

scientific article published on 01 September 1987

Two genes encoding proteins with similarities to rubredoxin and rubredoxin reductase are required for conversion of dodecane to lauric acid in Acinetobacter calcoaceticus ADP1 ⬇️

scientific article published on June 1, 1995

Two mutations in the tetracycline repressor change the inducer anhydrotetracycline to a corepressor

scientific article published on 4 February 2004

UDP-N-acetylglucosamine 1-carboxyvinyl-transferase from Acinetobacter calcoaceticus

scientific article published on April 1994

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