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List of works by Frank Buchholz

3D profile-based approach to proteome-wide discovery of novel human chemokines

scientific article

A domesticated transposon mediates the effects of a single-nucleotide polymorphism responsible for enhanced muscle growth

scientific journal article

A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia

scientific article

A genome-scale RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem cell identity

scientific article

A genome-wide RNAi screen identifies proteins modulating aberrant FLT3-ITD signaling ⬇️

scientific article published on March 19, 2013

A human interactome in three quantitative dimensions organized by stoichiometries and abundances

scientific article

A new logic for DNA engineering using recombination in Escherichia coli ⬇️

scientific article (publication date: October 1998)

A simple assay to determine the functionality of Cre or FLP recombination targets in genomic manipulation constructs.

scientific article published on August 1996

A single reporter mouse line for Vika, Flp, Dre, and Cre-recombination

scientific article published in Scientific Reports

A systematic RNAi synthetic interaction screen reveals a link between p53 and snoRNP assembly

scientific article

AML1 deletion in adult mice causes splenomegaly and lymphomas.

scientific article

Alteration of Cre recombinase site specificity by substrate-linked protein evolution.

scientific article published on November 2001

Alternative approaches for efficient inhibition of hepatitis C virus RNA replication by small interfering RNAs

scientific article published on April 2004

An RNA interference phenotypic screen identifies a role for FGF signals in colon cancer progression

scientific article published on 11 August 2011

An endoribonuclease-prepared siRNA screen in human cells identifies genes essential for cell division

scientific article

Analysis of Runx1 Using Induced Gene Ablation Reveals Its Essential Role in Pre-liver HSC Development and Limitations of an In Vivo Approach

Another brick in the wall: RNAi Screens identify new barriers in iPSC reprogramming

scientific article published on 01 August 2014

Antibody-coupled siRNA as an efficient method for in vivo mRNA knockdown

scientific article published on 3 December 2015

Antibody-mediated delivery of anti-KRAS-siRNA in vivo overcomes therapy resistance in colon cancer

scientific article

Antiviral therapy of persistent viral infection using genome editing

scientific article

Assessment and site-specific manipulation of DNA (hydroxy-)methylation during mouse corticogenesis

scientific article published on 27 February 2019

BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals

scientific article

CRISPR/Cas9 as a tool to dissect cancer mutations

scientific article published on 10 May 2019

CRISPR/Cas9 nickase-mediated disruption of hepatitis B virus open reading frame S and X.

scientific article published on 3 September 2015

Codon-improved Cre recombinase (iCre) expression in the mouse

scientific article (publication date: 2002)

Combined RNAi and localization for functionally dissecting long noncoding RNAs.

scientific article published on 12 February 2012

Comparative RNAi Screens in Isogenic Human Stem Cells Reveal SMARCA4 as a Differential Regulator

Comparative kinetic analysis of FLP and cre recombinases: mathematical models for DNA binding and recombination.

scientific article published in November 1998

Comparative profiling identifies C13orf3 as a component of the Ska complex required for mammalian cell division

scientific article

Comparison of detection methods for HPV status as a prognostic marker for loco-regional control after radiochemotherapy in patients with HNSCC.

scientific article published on 30 December 2017

Cre Recombinase and Other Tyrosine Recombinases

scientific article published on 10 May 2016

Crystal structure of a wild-type Cre recombinase-loxP synapse reveals a novel spacer conformation suggesting an alternative mechanism for DNA cleavage activation

scientific article

Crystal structure of an engineered, HIV-specific recombinase for removal of integrated proviral DNA.

scientific article published on September 2017

DEQOR: a web-based tool for the design and quality control of siRNAs

scientific article

Designing efficient and specific endoribonuclease-prepared siRNAs

scientific article published in January 2013

Development and validation of a gene signature for patients with head and neck squamous cell carcinomas treated by postoperative radio(chemo)therapy.

scientific article published on 3 January 2018

Development of a genetic sensor that eliminates p53 deficient cells

scientific article published on 13 November 2017

Different Effects of RNAi-Mediated Downregulation or Chemical Inhibition of NAMPT in an Isogenic IDH Mutant and Wild-Type Glioma Cell Model

scientific article published on 21 May 2022

Directed evolution of a recombinase that excises the provirus of most HIV-1 primary isolates with high specificity.

scientific article

Discovery of Nigri/nox and Panto/pox site-specific recombinase systems facilitates advanced genome engineering

scientific article published on 22 July 2016

Dissecting mechanisms of mouse embryonic stem cells heterogeneity through a model-based analysis of transcription factor dynamics

scientific article published on April 2016

Downregulation of PIK3CA via antibody-esiRNA-complexes suppresses human xenograft tumor growth.

scientific article published on 12 July 2018

Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice

scientific article

Efficient Generation and Correction of Mutations in Human iPS Cells Utilizing mRNAs of CRISPR Base Editors and Prime Editors

scientific article published on 06 May 2020

Engineered DNA modifying enzymes: components of a future strategy to cure HIV/AIDS

scientific article

Engineering DNA processing enzymes for the postgenomic era.

scientific article published on 21 August 2009

Engineering of a target site-specific recombinase by a combined evolution- and structure-guided approach

scientific article published on 28 December 2012

Enzymatically prepared RNAi libraries

scientific article

Erratum: Corrigendum: BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals

scholarly article published in Nature Methods

Erratum: Corrigendum: Systems survey of endocytosis by multiparametric image analysis

scientific article published in Nature

Excision of HIV-1 proviral DNA by recombinant cell permeable tre-recombinase

scientific article

From RNAi screens to molecular function in embryonic stem cells.

scientific article published on March 2012

Functional genomic analysis of cell division by endoribonuclease-prepared siRNAs

scientific article published on 19 April 2005

Genome-scale RNAi profiling of cell division in human tissue culture cells

scientific article

Genome-scale single-cell mechanical phenotyping reveals disease-related genes involved in mitotic rounding.

scientific article

Genome-wide resources of endoribonuclease-prepared short interfering RNAs for specific loss-of-function studies

scientific article

HAUS, the 8-subunit human Augmin complex, regulates centrosome and spindle integrity

scientific article

HIV-1 proviral DNA excision using an evolved recombinase

scientific article

HOT1 is a mammalian direct telomere repeat-binding protein contributing to telomerase recruitment

scientific article

HPV status, cancer stem cell marker expression, hypoxia gene signatures and tumour volume identify good prognosis subgroups in patients with HNSCC after primary radiochemotherapy: A multicentre retrospective study of the German Cancer Consortium Rad

scientific article published on 29 November 2016

High-throughput RNAi screening in mammalian cells with esiRNAs

scientific article published on 23 December 2010

Highly significant antiviral activity of HIV-1 LTR-specific tre-recombinase in humanized mice

scientific article published in 2013

Improved properties of FLP recombinase evolved by cycling mutagenesis ⬇️

scientific article published on July 1, 1998

In vitro evolution and analysis of HIV-1 LTR-specific recombinases

scientific article published on 26 June 2010

Inactivation of Cancer Mutations Utilizing CRISPR/Cas9.

scientific article published on 30 August 2016

Independent validation of the prognostic value of cancer stem cell marker expression and hypoxia-induced gene expression for patients with locally advanced HNSCC after postoperative radiotherapy.

scientific article

Independent validation of tumour volume, cancer stem cell markers and hypoxia-associated gene expressions for HNSCC after primary radiochemotherapy

article

Inducible chromosomal translocation of AML1 and ETO genes through Cre/loxP-mediated recombination in the mouse.

scientific article

Inducible expression based on regulated recombination: a single vector strategy for stable expression in cultured cells.

scientific article published on July 1998

Interaction between AP-5 and the hereditary spastic paraplegia proteins SPG11 and SPG15.

scientific article

Interplay of Acetyltransferase EP300 and the Proteasome System in Regulating Heat Shock Transcription Factor 1

article by Swasti Raychaudhuri et al published February 2014 in Cell

Low Cancer Stem Cell Marker Expression and Low Hypoxia Identify Good Prognosis Subgroups in HPV(-) HNSCC after Postoperative Radiochemotherapy: A Multicenter Study of the DKTK-ROG.

scientific article published on 11 January 2016

MISSION esiRNA for RNAi screening in mammalian cells

scientific article

MLLT6 maintains PD-L1 expression and mediates tumor immune resistance

scientific article published on 15 October 2020

MS Western, a method of multiplexed absolute protein quantification is a practical alternative to Western blotting.

scientific article published on 30 November 2017

Minimizing the risk of reporting false positives in large-scale RNAi screens

article

Molecular evolution of the tre recombinase

scientific article

Next-generation LTR-specific Tre-recombinase targets a majority of HIV-1 isolates

article

Nucleolar protein TAAP1/<i>C22orf46</i>confers pro-survival signaling in non-small cell lung cancer

scientific article published in 2024

Pairing of single mutations yields obligate Cre-type site-specific recombinases

scientific article published on 01 January 2022

PhenoFam-gene set enrichment analysis through protein structural information

scientific article

Phylointeractomics reconstructs functional evolution of protein binding

scientific article published on 08 February 2017

Polycomb group ring finger 1 cooperates with Runx1 in regulating differentiation and self-renewal of hematopoietic cells.

scientific article published on 12 March 2012

Production of Endoribonuclease-Prepared Short Interfering RNAs (esiRNAs) for Specific and Effective Gene Silencing in Mammalian Cells

scientific article published on 01 August 2007

Production of endoribonuclease-prepared short interfering RNAs for gene silencing in mammalian cells

article

Protein Dynamics in Complex DNA Lesions.

scientific article published in March 2018

Protein phosphatase 2A protects centromeric sister chromatid cohesion during meiosis I.

scientific article

Quantitative interaction screen of telomeric repeat-containing RNA reveals novel TERRA regulators.

scientific article

RAD21 cooperates with pluripotency transcription factors in the maintenance of embryonic stem cell identity

scientific article

RNA interference in postimplantation mouse embryos

RNA interference rescue by bacterial artificial chromosome transgenesis in mammalian tissue culture cells

scientific article

RNA interference: gene silencing in the fast lane

scientific article published on August 2003

RNAi in embryonic stem cells

scientific article published on January 2006

RNAi profiling of primary human AML cells identifies ROCK1 as a therapeutic target and nominates fasudil as an antileukemic drug

scientific article

RUNX1 DNA-binding mutants, associated with minimally differentiated acute myelogenous leukemia, disrupt myeloid differentiation

scientific article published in January 2007

Repeat FMISO-PET imaging weakly correlates with hypoxia-associated gene expressions for locally advanced HNSCC treated by primary radiochemotherapy

scientific article published on 09 March 2019

Revolutionizing HIV-therapy by Tre-Recombinase? Initial observations and current state of development

scientific article published in June 2013

Ribosome biogenesis and p53: who is regulating whom?

scientific article published in October 2011

Runx1 is essential at two stages of early murine B-cell development.

scientific article published on 23 May 2013

Runx1 is required for progression of CD41+ embryonic precursors into HSCs but not prior to this

scientific article published on September 2014

SKAnking with Ska3: essential role of Ska3 in cell division revealed by combined phenotypic profiling.

scientific article

STK3 is a therapeutic target for a subset of acute myeloid leukemias.

scientific article

SeLOX--a locus of recombination site search tool for the detection and directed evolution of site-specific recombination systems

scientific article published on 6 June 2010

Selective expression of the Cre recombinase in late-stage thymocytes using the distal promoter of the Lck gene.

scientific article published in June 2005

Short RNA duplexes produced by hydrolysis with Escherichia coli RNase III mediate effective RNA interference in mammalian cells

scientific article

Stage-specific binding profiles of cohesin in resting and activated B lymphocytes suggest a role for cohesin in immunoglobulin class switching and maturation

scientific article

Systematic analysis of human protein complexes identifies chromosome segregation proteins

scientific article

Systems Analyses Reveal Shared and Diverse Attributes of Oct4 Regulation in Pluripotent Cells

scientific article

Systems biology of mammalian cell division

scientific article published on 21 May 2008

Systems survey of endocytosis by multiparametric image analysis

scientific article published on 28 February 2010

Tandem affinity purification of functional TAP-tagged proteins from human cells

scientific article published on January 2007

Targeting Human Long Noncoding Transcripts by Endoribonuclease-Prepared siRNAs

scientific article published on 24 April 2015

The Apical Domain Is Required and Sufficient for the First Lineage Segregation in the Mouse Embryo.

scientific article published on February 2017

The FunGenES database: a genomics resource for mouse embryonic stem cell differentiation

scientific article

The Paf1 complex positively regulates enhancer activity in mouse embryonic stem cells

scientific article published on 29 December 2020

The coilin interactome identifies hundreds of small noncoding RNAs that traffic through Cajal bodies

scientific article published on 6 November 2014

The histone demethylase UTX regulates stem cell migration and hematopoiesis

scientific article

The long noncoding RNA lncR492 inhibits neural differentiation of murine embryonic stem cells.

scientific article

The symplekin/ZONAB complex inhibits intestinal cell differentiation by the repression of AML1/Runx1.

scientific article

Three LIF-dependent signatures and gene clusters with atypical expression profiles, identified by transcriptome studies in mouse ES cells and early derivatives

scientific article

Tissue-specific RNA interference in post-implantation mouse embryos using directional electroporation and whole embryo culture

scientific article published on March 2004

Tissue-specific RNA interference in postimplantation mouse embryos with endoribonuclease-prepared short interfering RNA

scientific article

Transcription factor Runx1 is pro-neurogenic in adult hippocampal precursor cells

scientific article published on 11 January 2018

Universal Tre (uTre) recombinase specifically targets the majority of HIV-1 isolates

scientific article published on 2 November 2014

Vika/vox, a novel efficient and specific Cre/loxP-like site-specific recombination system

scientific article published on 9 November 2012

ZBTB48 is both a vertebrate telomere-binding protein and a transcriptional activator

scientific article

Zfp281 orchestrates interconversion of pluripotent states by engaging Ehmt1 and Zic2

scientific article published on 29 November 2019

lncRNA Panct1 Maintains Mouse Embryonic Stem Cell Identity by Regulating TOBF1 Recruitment to Oct-Sox Sequences in Early G1.

scientific article

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