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List of works by Reinhard Georg Lührmann

3'-cyclic phosphorylation of U6 snRNA leads to recruitment of recycling factor p110 through LSm proteins

scientific article

3D cryo-EM structure of an active step I spliceosome and localization of its catalytic core

scientific article

5'-Terminal caps of snRNAs are reactive with antibodies specific for 2,2,7-trimethylguanosine in whole cells and nuclear matrices. Double-label immunofluorescent studies with anti-m3G antibodies and with anti-RNP and anti-Sm autoantibodies

scientific article published on October 1984

5'-terminal caps of snRNAs are accessible for reaction with 2,2,7-trimethylguanosine-specific antibody in intact snRNPs

scientific article published on 01 March 1983

A 69-kD protein that associates reversibly with the Sm core domain of several spliceosomal snRNP species.

scientific article published on February 1994

A common core RNP structure shared between the small nucleoar box C/D RNPs and the spliceosomal U4 snRNP

scientific article

A composite double-/single-stranded RNA-binding region in protein Prp3 supports tri-snRNP stability and splicing

scientific article

A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins.

scientific article

A cytoplasmically anchored nuclear protein interferes specifically with the import of nuclear proteins but not U1 snRNA.

scientific article published on April 1993

A doughnut-shaped heteromer of human Sm-like proteins binds to the 3'-end of U6 snRNA, thereby facilitating U4/U6 duplex formation in vitro

scientific article

A general approach for identification of RNA-protein cross-linking sites within native human spliceosomal small nuclear ribonucleoproteins (snRNPs). Analysis of RNA-protein contacts in native U1 and U4/U6.U5 snRNPs

scientific article

A major, novel systemic lupus erythematosus autoantibody class recognizes the E, F, and G Sm snRNP proteins as an E-F-G complex but not in their denatured states

scientific article published on 01 April 1997

A monoclonal antibody specific for snRNPs U1 and U2

scientific article published on 01 May 1986

A novel U2 and U11/U12 snRNP protein that associates with the pre-mRNA branch site.

scientific article

A novel yeast U2 snRNP protein, Snu17p, is required for the first catalytic step of splicing and for progression of spliceosome assembly

scientific article

A protein map of the yeast activated spliceosome as obtained by electron microscopy

scientific article published on July 2016

A role for eIF4E and eIF4E-transporter in targeting mRNPs to mammalian processing bodies

scientific article

A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity

scientific article published on 5 July 2016

A splicing factor that is inactivated during in vivo heat shock is functionally equivalent to the [U4/U6.U5] triple snRNP-specific proteins

scientific article published on April 1992

A stochastic view of spliceosome assembly and recycling in the nucleus

scientific article

A subset of human 35S U5 proteins, including Prp19, function prior to catalytic step 1 of splicing

scientific article

A two-tracked approach to analyze RNA-protein crosslinking sites in native, nonlabeled small nuclear ribonucleoprotein particles

scientific article

ATPγS stalls splicing after B complex formation but prior to spliceosome activation

scientific article

Amphibian transcription factor IIIA proteins contain a sequence element functionally equivalent to the nuclear export signal of human immunodeficiency virus type 1 Rev

scientific article

An Ancient Mechanism for Splicing Control: U11 snRNP as an Activator of Alternative Splicing

scientific article (publication date: March 2010)

An approach for de novo structure determination of dynamic molecular assemblies by electron cryomicroscopy

scientific article published in June 2010

An essential signaling role for the m3G cap in the transport of U1 snRNP to the nucleus

scientific article published on 01 August 1990

An evolutionarily conserved U5 snRNP-specific protein is a GTP-binding factor closely related to the ribosomal translocase EF-2 ⬇️

scientific article published on July 1, 1997

An in vivo and in vitro structure-function analysis of the Saccharomyces cerevisiae U3A snoRNP: protein-RNA contacts and base-pair interaction with the pre-ribosomal RNA 1 1Edited by M. Yaniv ⬇️

scientific article published on October 31, 1997

An unusual RNA recognition motif acts as a scaffold for multiple proteins in the pre-mRNA retention and splicing complex

scientific article published on 22 September 2008

An unusual chemical reactivity of Sm site adenosines strongly correlates with proper assembly of core U snRNP particles

scientific article published in January 1999

Analysis of genomic clones of the murine U1RNA-associated 70-kDa protein reveals a high evolutionary conservation of the protein between human and mouse

scientific article

Analyzing RNA-protein crosslinking sites in unlabeled ribonucleoprotein complexes by mass spectrometry

scientific article

Analyzing the Protein Assembly and Dynamics of the Human Spliceosome with SILAC

Anti-idiotype RNA selected with an anti-nuclear export signal antibody is actively transported in oocytes and inhibits Rev- and cap-dependent RNA export ⬇️

scientific article published on November 25, 1997

Antibodies specific for N6-methyladenosine react with intact snRNPs U2 and U4/U6

scientific article published on 01 March 1987

Antisense-targeted immuno-EM localization of the pre-mRNA path in the spliceosomal C complex

scientific article published on 24 May 2012

Arrangement of RNA and proteins in the spliceosomal U1 small nuclear ribonucleoprotein particle

scientific article (publication date: 25 January 2001)

Assembly and maturation of the U3 snoRNP in the nucleoplasm in a large dynamic multiprotein complex

scientific article

Autoantibodies to ribonucleoprotein particles containing U2 small nuclear RNA.

scientific article published on June 1985

Autoantibody production against the U small nuclear ribonucleoprotein particle proteins E, F and G in patients with connective tissue diseases

scientific article published on 01 February 1990

Binding of the human Prp31 Nop domain to a composite RNA-protein platform in U4 snRNP

scientific article

Biochemical and NMR analyses of an SF3b155-p14-U2AF-RNA interaction network involved in branch point definition during pre-mRNA splicing

scientific article published on March 2006

Blocking of the initiation of protein biosynthesis by a pentanucleotide complementary to the 3' end of Escherichia coli 16 S rRNA ⬇️

scientific article published on November 25, 1979

CRM1-mediated recycling of snurportin 1 to the cytoplasm

scientific article

Cbf5p, a potential pseudouridine synthase, and Nhp2p, a putative RNA-binding protein, are present together with Gar1p in all H BOX/ACA-motif snoRNPs and constitute a common bipartite structure.

scientific article

Characterisation of human and murine snRNP proteins by two-dimensional gel electrophoresis and phosphopeptide analysis of U1-specific 70K protein variants

scientific article published on August 1990

Characterization of an HLA-DR4-restricted T cell clone recognizing a protein moiety of small nuclear ribonucleoproteins (UsnRNP).

scientific article published on March 1994

Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein

scientific article

Characterization of purified human Bact spliceosomal complexes reveals compositional and morphological changes during spliceosome activation and first step catalysis

scientific article

Cloning of the human cDNA for the U1 RNA-associated 70K protein.

scientific article

Codon-anticodon interaction at the ribosomal peptidyl-site

scientific article published on August 2, 1979

Coilin-dependent snRNP assembly is essential for zebrafish embryogenesis

scientific article published on 28 March 2010

Common Design Principles in the Spliceosomal RNA Helicase Brr2 and in the Hel308 DNA Helicase

Common design principles in the spliceosomal RNA helicase Brr2 and in the Hel308 DNA helicase.

scientific article

Complete MALDI-ToF MS analysis of cross-linked peptide-RNA oligonucleotides derived from nonlabeled UV-irradiated ribonucleoprotein particles

scientific article

Composition and three-dimensional EM structure of double affinity-purified, human prespliceosomal A complexes

scientific article

Conservation between yeast and man of a protein associated with U5 small nuclear ribonucleoprotein

scientific article

Conservation of the protein composition and electron microscopy structure of Drosophila melanogaster and human spliceosomal complexes

scientific article

Conserved loop I of U5 small nuclear RNA is dispensable for both catalytic steps of pre-mRNA splicing in HeLa nuclear extracts.

scientific article

Conserved stem II of the box C/D motif is essential for nucleolar localization and is required, along with the 15.5K protein, for the hierarchical assembly of the box C/D snoRNP

scientific article

Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex

scientific article

Coupled in vitro import of U snRNPs and SMN, the spinal muscular atrophy protein

scientific article

Covalent binding of uridine-oligonucleotides to 70 SE. Coliribosomes ⬇️

scientific article published on May 15, 1973

Crosslinking of the U5 snRNP-specific 116-kDa protein to RNA hairpins that block step 2 of splicing ⬇️

scientific article published on November 1, 1997

Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation

scientific article published on 25 July 2017

Cryo-EM structure of a human spliceosome activated for step 2 of splicing

scientific article published on 11 January 2017

Cryo-electron microscopy of spliceosomal components

scientific article

Crystal structure of Cwc2 reveals a novel architecture of a multipartite RNA-binding protein ⬇️

scientific article published on March 9, 2012

Crystal structure of a complex between human spliceosomal cyclophilin H and a U4/U6 snRNP-60K peptide

scientific article

Crystal structure of a core spliceosomal protein interface

scholarly article

Crystal structure of the Pml1p subunit of the yeast precursor mRNA retention and splicing complex

scientific article

Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment

scientific article

Crystal structures of two Sm protein complexes and their implications for the assembly of the spliceosomal snRNPs

scientific article

Cwc2 and its human homologue RBM22 promote an active conformation of the spliceosome catalytic centre ⬇️

scientific article published on January 13, 2012

Cytochemical and immunocytochemical characterization of nuclear bodies during hibernation

scientific article published on 01 October 1994

Decoding at the ribosomal A site. The effect of a defined codon-anticodon mismatch upon the behavior of bound aminoacyl transfer RNA ⬇️

scientific article published on May 10, 1984

Detecting protein-induced folding of the U4 snRNA kink-turn by single-molecule multiparameter FRET measurements

scientific article published on October 2005

Detection of antibodies to extractable nuclear antigens with counterimmunoelectrophoresis and the Western blot technic

scientific article published on 01 January 1988

Determination of protein stoichiometry within protein complexes using absolute quantification and multiple reaction monitoring

scientific article

Dinucleotide codon-anticodon interaction as a minimum requirement for ribosomal as-tRNA binding: stabilisation by viomycin of aa-tRNA in the A site ⬇️

scientific article published on December 11, 1980

Direct binding of small nuclear ribonucleoprotein G to the Sm site of small nuclear RNA. Ultraviolet light cross-linking of protein G to the AAU stretch within the Sm site (AAUUUGUGG) of U1 small nuclear ribonucleoprotein reconstituted in vitro

scientific article published on September 5, 1992

Direct cross-linking of snRNP proteins F and 70K to snRNAs by ultra-violet radiation in situ

scientific article

Direct probing of RNA structure and RNA-protein interactions in purified HeLa cell's and yeast spliceosomal U4/U6.U5 tri-snRNP particles

scientific article (publication date: 12 April 2002)

Discriminatory RNP remodeling by the DEAD-box protein DED1.

scientific article

Dissection of the factor requirements for spliceosome disassembly and the elucidation of its dissociation products using a purified splicing system

scientific article published on February 2013

Diversity in the signals required for nuclear accumulation of U snRNPs and variety in the pathways of nuclear transport

scientific article published on May 1991

Dynamic Contacts of U2, RES, Cwc25, Prp8 and Prp45 Proteins with the Pre-mRNA Branch-Site and 3' Splice Site during Catalytic Activation and Step 1 Catalysis in Yeast Spliceosomes

scientific article

Dynamic protein-protein interaction wiring of the human spliceosome

scientific article

Dynamic regulation of alternative splicing by silencers that modulate 5' splice site competition

scientific article published on December 2008

Effect of codon shortening and the antibiotics viomycin and sparsomycin upon the behaviour of bound aminoacyl-tRNA. Decoding at the ribosomal A site

scientific article

Electron microscopy of U1 small nuclear ribonucleoprotein particles: shape of the particle and position of the 5' RNA terminus

scientific article

Electron microscopy of U4/U6 snRNP reveals a Y-shaped U4 and U6 RNA containing domain protruding from the U4 core RNP.

scientific article published on March 1991

Electron microscopy of assembly intermediates of the snRNP core: morphological similarities between the RNA-free (E.F.G) protein heteromer and the intact snRNP core

scientific article

Electron microscopy of small nuclear ribonucleoprotein (snRNP) particles U2 and U5: evidence for a common structure-determining principle in the major U snRNP family

scientific article

Electron microscopy of snRNPs U2, U4/6 and U5: evidence for a common structure-determining principle in the major UsnRNP family

scientific article published on 01 January 1990

Essential role for the tudor domain of SMN in spliceosomal U snRNP assembly: implications for spinal muscular atrophy

scientific article (publication date: December 1999)

Evidence for the existence of snRNAs U4 and U6 in a single ribonucleoprotein complex and for their association by intermolecular base pairing

scientific article

Evidence from complementation assays in vitro that U5 snRNP is required for both steps of mRNA splicing

scientific article published on October 1989

Evidence that HIV-1 Rev directly promotes the nuclear export of unspliced RNA

scientific journal article

Exon definition complexes contain the tri-snRNP and can be directly converted into B-like precatalytic splicing complexes

scientific article published in April 2010

Exon, intron and splice site locations in the spliceosomal B complex

scientific article published on 18 June 2009

Functional interaction of a novel 15.5kD [U4/U6.U5] tri-snRNP protein with the 5' stem-loop of U4 snRNA

scientific article

Functional organization of the Sm core in the crystal structure of human U1 snRNP

scientific article

Functional spliceosomal A complexes can be assembled in vitro in the absence of a penta-snRNP.

scientific article published on 31 July 2006

Functions of U-snRNPs

scientific article published on January 1990

GraFix: sample preparation for single-particle electron cryomicroscopy

scientific article

HSPC117 is the essential subunit of a human tRNA splicing ligase complex

scientific article

Hierarchical, clustered protein interactions with U4/U6 snRNA: a biochemical role for U4/U6 proteins

scientific article

High intranuclear mobility and dynamic clustering of the splicing factor U1 snRNP observed by single particle tracking

scientific article

Human PRP4 kinase is required for stable tri-snRNP association during spliceosomal B complex formation

scientific article published on 31 January 2010

Human U4/U6 snRNP recycling factor p110: mutational analysis reveals the function of the tetratricopeptide repeat domain in recycling

scientific article

Human U4/U6.U5 and U4atac/U6atac.U5 tri-snRNPs exhibit similar protein compositions

scientific article published on May 2002

Identification and characterization of novel Sm-autoantigens associated with the major snRNPs U1, U2, U4/U6 and U5 ⬇️

scientific article published on 01 January 1990

Identification by mass spectrometry and functional analysis of novel proteins of the yeast [U4/U6.U5] tri-snRNP.

scientific article published in August 1999

Identification of a small molecule inhibitor that stalls splicing at an early step of spliceosome activation.

scientific article

Identification of an RNA-dependent ATPase activity in mammalian U5 snRNPs

scientific article

Identification of an evolutionarily divergent U11 small nuclear ribonucleoprotein particle in Drosophila

scientific article

Identification of an snRNP-associated kinase activity that phosphorylates arginine/serine rich domains typical of splicing factors

scientific article

Identification of both shared and distinct proteins in the major and minor spliceosomes

scientific article published on June 1999

Identification of novel argonaute-associated proteins

scientific article

Identification of the proteins of the yeast U1 small nuclear ribonucleoprotein complex by mass spectrometry

scientific article

Identification, cloning, and functional analysis of the human U6 snRNA-specific terminal uridylyl transferase

scientific article

Immunization of mice with purified U1 small nuclear ribonucleoprotein (RNP) induces a pattern of antibody specificities characteristic of the anti-Sm and anti-RNP autoimmune response of patients with lupus erythematosus, as measured by monoclonal an

scientific article

Immuno affinity purification and characterization of a stable U4/U5/U6 multip-snRNP complex from HeLa nuclear extracts

scientific article published on 01 January 1990

Immunoaffinity purification of a [U4/U6.U5] tri-snRNP from human cells ⬇️

scientific article published on August 1, 1991

Immunocytochemical characterization of nuclear ribonucleoprotein fibrils in cells of the central nervous system of the rat

scientific article published on 01 December 1994

Improved identification of enriched peptide RNA cross-links from ribonucleoprotein particles (RNPs) by mass spectrometry

scientific article

In vitro and in vivo evidence that protein and U1 snRNP nuclear import in somatic cells differ in their requirement for GTP-hydrolysis, Ran/TC4 and RCC1

scientific article published on May 1996

In vitro nuclear import of snRNPs: cytosolic factors mediate m3G-cap dependence of U1 and U2 snRNP transport.

scientific article

In vitro reconstitution of U1 and U2 snRNPs from isolated proteins and snRNA

scientific article published on 01 September 1992

In vitro reconstitution of mammalian U1 snRNPs active in splicing: the U1-C protein enhances the formation of early (E) spliceosomal complexes

scientific article

In vitro reconstitution of mammalian U2 and U5 snRNPs active in splicing: Sm proteins are functionally interchangeable and are essential for the formation of functional U2 and U5 snRNPs ⬇️

scientific article published on August 15, 1995

Inhibition of RNA helicase Brr2 by the C-terminal tail of the spliceosomal protein Prp8

scientific article

Interaction of mammalian splicing factor SF3a with U2 snRNP and relation of its 60-kD subunit to yeast PRP9

scientific article published on 01 October 1993

Interaction of the human autoantigen p150 with splicing snRNPs

scientific article published on 01 July 1993

Involvement of nuclear import and export factors in U8 box C/D snoRNP biogenesis

scientific article

Is the coiled body involved in nucleolar functions?

scientific article published on 01 April 1994

Isolation and characterization of rabbit anti-m3 2,2,7G antibodies

scientific article

Isolation and characterization of the small nucleolar ribonucleoprotein particle snR30 from Saccharomyces cerevisiae

scientific article published on 01 May 1995

Isolation and partial characterization of U1-U6 small RNAs from Bombyx mori

scientific article

Isolation of S. cerevisiae snRNPs: comparison of U1 and U4/U6.U5 to their human counterparts

scientific article published on 01 April 1994

Isolation of U3 snoRNP from CHO cells: a novel 55 kDa protein binds to the central part of U3 snoRNA

scientific article

Isolation of an active step I spliceosome and composition of its RNP core

scientific article published on 5 March 2008

Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy

scientific article

Localization of a base-paired interaction between small nuclear RNAs U4 and U6 in intact U4/U6 ribonucleoprotein particles by psoralen cross-linking

scientific article published on 01 October 1985

Localization of the puromycin binding site on the large ribosomal subunit of Escherichia coli by immunoelectron microscopy

scientific article

Macromolecular-scale resolution in biological fluorescence microscopy

scientific article

Major Conformational Change in the Complex SF3b upon Integration into the Spliceosomal U11/U12 di-snRNP as Revealed by Electron Cryomicroscopy

scientific article (publication date: March 2005)

Mammalian PRP4 kinase copurifies and interacts with components of both the U5 snRNP and the N-CoR deacetylase complexes

scientific article

Mass spectrometry-based relative quantification of proteins in precatalytic and catalytically active spliceosomes by metabolic labeling (SILAC), chemical labeling (iTRAQ), and label-free spectral count

scientific article published on 21 January 2014

Mercuric chloride induces autoantibodies against U3 small nuclear ribonucleoprotein in susceptible mice

scientific article

Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA and nuclear pores ⬇️

scientific article published on June 2, 1997

Minor spliceosome components are predominantly localized in the nucleus

scientific article

Modified nucleotides at the 5' end of human U2 snRNA are required for spliceosomal E-complex formation

scientific article

Molecular Architecture of SF3b and Structural Consequences of Its Cancer-Related Mutations

scientific article

Molecular Architecture of the Multiprotein Splicing Factor SF3b ⬇️

scientific article (publication date: 9 May 2003)

Molecular architecture of the Saccharomyces cerevisiae activated spliceosome

scientific article

Molecular architecture of the human Prp19/CDC5L complex

scientific article

Molecular architecture of the human U4/U6.U5 tri-snRNP

scientific article

Molecular architecture of zinc chelating small molecules that inhibit spliceosome assembly at an early stage

scientific article published on 25 July 2012

Molecular biology. RNP remodeling with DExH/D boxes

scientific article published in March 2001

Molecular cloning and subcellular localisation of the snRNP-associated protein 69KD, a structural homologue of the proto-oncoproteins TLS and EWS with RNA and DNA-binding properties

scientific article published on 01 December 1996

Molecular dissection of step 2 catalysis of yeast pre-mRNA splicing investigated in a purified system

scientific article

Molecular relationships between U snRNP proteins as investigated by rabbit antisera and peptide mapping

scientific article published on May 1987

Multiple RNA-RNA tertiary interactions are dispensable for formation of a functional U2/U6 RNA catalytic core in the spliceosome

scientific article published on 01 December 2018

Multiple protein-protein interactions converging on the Prp38 protein during activation of the human spliceosome

scientific article published on 16 December 2015

Novel regulatory principles of the spliceosomal Brr2 RNA helicase and links to retinal disease in humans

scientific article published on 05 March 2014

Novel structure of a human U6 snRNA pseudogene

scientific article

Nuclear transport of U1 snRNP in somatic cells: differences in signal requirement compared with Xenopus laevis oocytes

scientific article published on June 1994

Nucleo-cytoplasmic transport of U snRNPs: definition of a nuclear location signal in the Sm core domain that binds a transport receptor independently of the m3G cap ⬇️

scientific article published on February 1, 1993

On the mRNA induced conformational change of AA-tRNA exposing the T-Ψ-C-G sequence for binding to the 50S ribosomal subunit ⬇️

scientific article published on February 4, 1974

Ongoing U snRNP biogenesis is required for the integrity of Cajal bodies

scientific article

Organization of core spliceosomal components U5 snRNA loop I and U4/U6 Di-snRNP within U4/U6.U5 Tri-snRNP as revealed by electron cryomicroscopy

scientific article

Phosphorylation drives a dynamic switch in serine/arginine-rich proteins

scientific article published on 31 October 2013

Phosphorylation of human PRP28 by SRPK2 is required for integration of the U4/U6-U5 tri-snRNP into the spliceosome

scientific article published on 20 April 2008

Phylogenetic conservation of modified nucleotides in the terminal loop 1 of the spliceosomal U5 snRNA ⬇️

scientific article published on January 1, 1995

Polypeptide components ofDrosophilasmall nuclear ribonucleoprotein particles ⬇️

scientific article published on November 11, 1991

Post-transcriptional spliceosomes are retained in nuclear speckles until splicing completion

scientific article

Proline-rich sequence recognition: I. Marking GYF and WW domain assembly sites in early spliceosomal complexes

scientific article

Protein 61K, encoded by a gene (PRPF31) linked to autosomal dominant retinitis pigmentosa, is required for U4/U6*U5 tri-snRNP formation and pre-mRNA splicing

scientific article

Protein cofactor competition regulates the action of a multifunctional RNA helicase in different pathways

scientific article published on 29 January 2016

Protein composition and electron microscopy structure of affinity-purified human spliceosomal B complexes isolated under physiological conditions

scientific article published on July 2006

Protein composition of human mRNPs spliced in vitro and differential requirements for mRNP protein recruitment

scientific article published on 9 November 2006

Protein composition of human prespliceosomes isolated by a tobramycin affinity-selection method

scientific article

Protein composition of mammalian spliceosomal snRNPs

scientific article

Protein functions in pre-mRNA splicing

scientific article (publication date: June 1997)

Protein localisation by electron microscopy reveals the architecture of the yeast spliceosomal B complex

scientific article published on 18 November 2015

Protein stoichiometry of a multiprotein complex, the human spliceosomal U1 small nuclear ribonucleoprotein: absolute quantification using isotope-coded tags and mass spectrometry

scientific article

Protein-RNA interactions in 20S U5 snRNPs ⬇️

scientific article published on January 17, 1991

Protein-protein and protein-RNA contacts both contribute to the 15.5K-mediated assembly of the U4/U6 snRNP and the box C/D snoRNPs

scientific article published on July 2006

Protein-protein interactions and 5'-splice-site recognition in mammalian mRNA precursors

scientific article

Proximity of conserved U6 and U2 snRNA elements to the 5' splice site region in activated spliceosomes

scientific article published on 11 May 2006

Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors

scientific article published in March 2018

Prp2-mediated protein rearrangements at the catalytic core of the spliceosome as revealed by dcFCCS.

scientific article

Psoromic acid derivatives: a new family of small-molecule pre-mRNA splicing inhibitors discovered by a stage-specific high-throughput in vitro splicing assay

scientific article

Purification and characterization of human autoantibodies directed to specific regions on U1RNA; recognition of native U1RNP complexes

scientific article published on November 1993

Purification of U small nuclear ribonucleoprotein particles

scientific article published on 01 January 1999

Purification of snRNPs U1, U2, U4, U5 and U6 with 2,2,7-trimethylguanosine-specific antibody and definition of their constituent proteins reacting with anti-Sm and anti-(U1)RNP antisera ⬇️

scientific article published on January 1, 1983

Purification of the individual snRNPs U1, U2, U5 and U4/U6 from HeLa cells and characterization of their protein constituents

scientific article

Purification of the major UsnRNPs from broad bean nuclear extracts and characterization of their protein constituents

scientific article published in February 1989

Purification, crystallization and preliminary crystallographic data of the m3G cap-binding domain of human snRNP import factor snurportin 1.

scientific article

RNA structural requirements for the association of the spliceosomal hPrp31 protein with the U4 and U4atac small nuclear ribonucleoproteins

scientific article

RNA structure analysis of human spliceosomes reveals a compact 3D arrangement of snRNAs at the catalytic core

scientific article

RNA structure and RNA-protein interactions in purified yeast U6 snRNPs

scientific article

RNAi knockdown of hPrp31 leads to an accumulation of U4/U6 di-snRNPs in Cajal bodies

scientific article

Recognition of termination codon by release factor in the presence of a tRNA-occupied A site. Evidence for flexibility in accommodation of the release factor on the ribosome

scientific article published in Journal of Biological Chemistry

Reconstitution of both steps of Saccharomyces cerevisiae splicing with purified spliceosomal components

scientific article

Regulation of Prp43-mediated disassembly of spliceosomes by its cofactors Ntr1 and Ntr2

scientific article published on 6 December 2016

Rev-mediated nuclear export of RNA is dominant over nuclear retention and is coupled to the Ran-GTPase cycle.

scientific article published on November 1999

Role of pre-rRNA base pairing and 80S complex formation in subnucleolar localization of the U3 snoRNP

scientific article

SUMO conjugation to spliceosomal proteins is required for efficient pre-mRNA splicing

scientific article published on 30 March 2017

Semiquantitative proteomic analysis of the human spliceosome via a novel two-dimensional gel electrophoresis method

scientific article

Signal-mediated nuclear export pathways of proteins and RNAs.

scientific article published in August 1996

Single-stranded antisense siRNAs guide target RNA cleavage in RNAi

scientific article (publication date: 6 September 2002)

Sm protein-Sm site RNA interactions within the inner ring of the spliceosomal snRNP core structure

scientific article

Small nuclear ribonucleoprotein (RNP) U2 contains numerous additional proteins and has a bipartite RNP structure under splicing conditions

scientific article published on January 1993

Small nuclear ribonucleoprotein remodeling during catalytic activation of the spliceosome

scientific article

SnapShot: Spliceosome Dynamics I

article

SnapShot: Spliceosome Dynamics II.

scientific article

SnapShot: Spliceosome Dynamics III.

scientific article published on July 2015

Snurportin1, an m3G-cap-specific nuclear import receptor with a novel domain structure ⬇️

scientific article published on July 15, 1998

Solution structure of human U1 snRNA. Derivation of a possible three-dimensional model

scientific article published on July 1990

Spliceosomal U snRNP core assembly: Sm proteins assemble onto an Sm site RNA nonanucleotide in a specific and thermodynamically stable manner

scientific article

Spliceosomal UsnRNP biogenesis, structure and function

scientific article (publication date: June 2001)

Splicing of a rare class of introns by the U12-dependent spliceosome

scientific article

Stable tri-snRNP integration is accompanied by a major structural rearrangement of the spliceosome that is dependent on Prp8 interaction with the 5' splice site

scientific article published on 18 September 2015

Stalling of spliceosome assembly at distinct stages by small-molecule inhibitors of protein acetylation and deacetylation

scientific article

Structural Basis of Splicing Modulation by Antitumor Macrolide Compounds

scientific article published on 24 March 2018

Structural and functional analysis of the RNA helicase Prp43 from the thermophilic eukaryote Chaetomium thermophilum

scientific article published on 22 January 2016

Structural and functional analysis of the human spliceosomal DEAD-box helicase Prp28

scientific article

Structural basis for dual roles of Aar2p in U5 snRNP assembly

scientific article

Structural basis for functional cooperation between tandem helicase cassettes in Brr2-mediated remodeling of the spliceosome

scientific article

Structural basis for m3G-cap-mediated nuclear import of spliceosomal UsnRNPs by snurportin1

scientific article

Structural basis for the bifunctionality of the U5 snRNP 52K protein (CD2BP2)

scientific article

Structural basis for the dual U4 and U4atac snRNA-binding specificity of spliceosomal protein hPrp31

scientific article

Structural insights into the mechanism of the DEAH-box RNA helicase Prp43

scientific article published on 16 January 2017

Structural mapping of spliceosomes by electron microscopy

scientific article published on 09 February 2009

Structure and Conformational Dynamics of the Human Spliceosomal Bact Complex

scientific article published on 12 January 2018

Structure and function of an RNase H domain at the heart of the spliceosome

scientific article

Structure of a multipartite protein-protein interaction domain in splicing factor prp8 and its link to retinitis pigmentosa

scientific article

Structure of spliceosomal snRNPs and their role in pre-mRNA splicing

scientific article

Structure of the small nuclear RNP particle U1: identification of the two structural protuberances with RNP-antigens A and 70K ⬇️

scientific article published on February 1, 1992

Structure of yeast U6 snRNPs: arrangement of Prp24p and the LSm complex as revealed by electron microscopy

scientific article

Structure-probing of U1 snRNPs gradually depleted of the U1-specific proteins A, C and 70k. Evidence that A interacts differentially with developmentally regulated mouse U1 snRNA variants

scientific article published on February 1990

Symmetrical dimethylation of arginine residues in spliceosomal Sm protein B/B' and the Sm-like protein LSm4, and their interaction with the SMN protein.

scientific article

Synthesis and characterization of pseudocantharidins, novel phosphatase modulators that promote the inclusion of exon 7 into the SMN (survival of motoneuron) pre-mRNA

scientific article

T cell lines recognizing the 70-kD protein of U1 small nuclear ribonucleoprotein (U1snRNP) ⬇️

scientific article published on September 1, 1995

The 20kD protein of human [U4/U6.U5] tri-snRNPs is a novel cyclophilin that forms a complex with the U4/U6-specific 60kD and 90kD proteins ⬇️

scientific article published on February 1, 1998

The 5′ terminus of the RNA moiety of U1 small nuclear ribonucleoprotein particles is required for the splicing of messenger RNA precursors ⬇️

scientific article published on 01 August 1984

The 5′-terminal sequence of U1 RNA complementary to the consensus 5′ splice site of hnRNA is single-stranded in intact U1 snRNP particles ⬇️

scientific article published on May 25, 1984

The C-terminal RG dipeptide repeats of the spliceosomal Sm proteins D1 and D3 contain symmetrical dimethylarginines, which form a major B-cell epitope for anti-Sm autoantibodies

scientific article (publication date: 2 June 2000)

The G-patch protein Spp2 couples the spliceosome-stimulated ATPase activity of the DEAH-box protein Prp2 to catalytic activation of the spliceosome

scientific article

The HIV-1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs

scientific article (publication date: 11 August 1995)

The HeLa 200 kDa U5 snRNP-specific protein and its homologue in Saccharomyces cerevisiae are members of the DEXH-box protein family of putative RNA helicases

scientific article

The Prp19 complex directly functions in mitotic spindle assembly

scientific article (publication date: 2013)

The Prp8 RNase H-like domain inhibits Brr2-mediated U4/U6 snRNA unwinding by blocking Brr2 loading onto the U4 snRNA.

scientific article published on November 2012

The RES complex is required for efficient transformation of the precatalytic B spliceosome into an activated Bact complex

scientific article published on 12 January 2018

The RNA helicase Aquarius exhibits structural adaptations mediating its recruitment to spliceosomes

scientific article

The Shine and Dalgarno hypothesis for termination: the 3' terminus of the 16S rRNA of the Escherichia coli ribosome can be modified or base-paired with a complementary oligonucleotide without affecting termination in vitro.

scientific article published on October 1983

The Sm domain is an ancient RNA-binding motif with oligo(U) specificity

scientific article

The Sm-core mediates the retention of partially-assembled spliceosomal snRNPs in Cajal bodies until their full maturation

scientific article published on 5 February 2018

The U11-48K protein contacts the 5' splice site of U12-type introns and the U11-59K protein

scientific article

The U11/U12 snRNP 65K protein acts as a molecular bridge, binding the U12 snRNA and U11-59K protein

scientific article

The [U4/U6.U5] tri-snRNP-specific 27K protein is a novel SR protein that can be phosphorylated by the snRNP-associated protein kinase

scientific article published on April 1997

The association of the U1-specific 70K and C proteins with U1 snRNPs is mediated in part by common U snRNP proteins

scientific article

The crystal structure of PPIL1 bound to cyclosporine A suggests a binding mode for a linear epitope of the SKIP protein

scientific article

The evolutionarily conserved core design of the catalytic activation step of the yeast spliceosome

scientific article

The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome

scientific article

The human LSm1-7 proteins colocalize with the mRNA-degrading enzymes Dcp1/2 and Xrnl in distinct cytoplasmic foci

scientific article

The human U4/U6 snRNP contains 60 and 90kD proteins that are structurally homologous to the yeast splicing factors Prp4p and Prp3p ⬇️

scientific article published on August 1, 1997

The human U5 snRNP 52K protein (CD2BP2) interacts with U5-102K (hPrp6), a U4/U6.U5 tri-snRNP bridging protein, but dissociates upon tri-snRNP formation

scientific article

The human U5 snRNP-specific 100-kD protein is an RS domain-containing, putative RNA helicase with significant homology to the yeast splicing factor Prp28p ⬇️

scientific article published on November 1, 1997

The human U5-200kD DEXH-box protein unwinds U4/U6 RNA duplicesin vitro ⬇️

scientific article published on April 14, 1998

The human U5-220kD protein (hPrp8) forms a stable RNA-free complex with several U5-specific proteins, including an RNA unwindase, a homologue of ribosomal elongation factor EF-2, and a novel WD-40 protein

scientific article

The importin-beta binding domain of snurportin1 is responsible for the Ran- and energy-independent nuclear import of spliceosomal U snRNPs in vitro

scientific article published on 28 January 2002

The intronic splicing code: multiple factors involved in ATM pseudoexon definition

scientific article

The large N-terminal region of the Brr2 RNA helicase guides productive spliceosome activation

scientific article published on 4 December 2015

The mammalian homologue of Prp16p is overexpressed in a cell line tolerant to Leflunomide, a new immunoregulatory drug effective against rheumatoid arthritis ⬇️

scientific article published on August 1, 1998

The network of protein-protein interactions within the human U4/U6.U5 tri-snRNP

scientific article

The ribosomal translocase homologue Snu114p is involved in unwinding U4/U6 RNA during activation of the spliceosome

scientific article published on 16 August 2002

The snRNP core assembly pathway: identification of stable core protein heteromeric complexes and an snRNP subcore particle in vitro

scientific article

The spliceosome: design principles of a dynamic RNP machine

scientific article (publication date: 20 February 2009)

The target of the DEAH-box NTP triphosphatase Prp43 in Saccharomyces cerevisiae spliceosomes is the U2 snRNP-intron interaction.

scientific article published on 26 April 2016

The thermolability of nuclear protein import in tsBN2 cells is suppressed by microinjected Ran-GTP or Ran-GDP, but not by RanQ69L or RanT24N.

scientific article published on June 1996

The yeast U5 snRNP coisolated with the U1 snRNP has an unexpected protein composition and includes the splicing factor Aar2p

scientific article

Thiophosphorylation of U1-70K protein inhibits pre-mRNA splicing

scientific article published in Nature

Three-dimensional structure of a pre-catalytic human spliceosomal complex B.

scientific article published on 18 April 2004

Through the glass lightly

Tobramycin affinity tag purification of spliceosomes

scientific article published in January 2004

Two protein-protein interaction sites on the spliceosome-associated human cyclophilin CypH

scientific article

U1 snRNP-ASF/SF2 interaction and 5' splice site recognition: characterization of required elements.

scientific article published on August 1995

U1, U2 and U5 small nuclear RNAs are found in plants cells. Complete nucleotide sequence of the U5 RNA family from pea nuclei ⬇️

scientific article published on December 20, 1983

U1-specific protein C is required for efficient complex formation of U1 snRNP with a 5' splice site

scientific article published on 01 January 1990

U1-specific protein C needed for efficient complex formation of U1 snRNP with a 5' splice site

scientific article

U2 snRNA-protein contacts in purified human 17S U2 snRNPs and in spliceosomal A and B complexes

scientific article published on April 2006

Unique Sm core structure of U7 snRNPs: assembly by a specialized SMN complex and the role of a new component, Lsm11, in histone RNA processing

scientific article

Yeast Prp2 liberates the 5' splice site and the branch site adenosine for catalysis of pre-mRNA splicing

scientific article published on September 2017

Zinc finger-like structure in U1-specific protein C is essential for specific binding to U1 snRNP ⬇️

scientific article published on February 11, 1991

cDNA Cloning and Characterization of the Human U3 Small Nucleolar Ribonucleoprotein Complex-Associated 55-Kilodalton Protein ⬇️

scientific article published on January 1, 1998

snRNP Sm proteins share two evolutionarily conserved sequence motifs which are involved in Sm protein-protein interactions

scientific article

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