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List of works by Tsutomu Arakawa

A Novel In Vitro Model for Screening and Evaluation of Anti-asthenopia Drugs ⬇️

scientific article published on October 1, 2003

A convenient rabbit model of ocular epithelium damage induced by osmotic dehydration

scientific article

A magic agent in protein refolding: arginine

scientific article

A novel "reverse screening" to identify refolding additives for activin-A.

scientific article

A single Gly114Arg mutation stabilizes the hexameric subunit assembly and changes the substrate specificity of halo-archaeal nucleoside diphosphate kinase

scientific article published on 27 July 2007

A study of the small-molecule system used to investigate the effect of arginine on antibody elution in hydrophobic charge-induction chromatography

scientific article published on 10 September 2016

Activation of halophilic nucleoside diphosphate kinase by a non-ionic osmolyte, trimethylamine N-oxide

scientific article published in May 2003

Active form of neuroprotective Humanin, HN, and inactive analog, S7A-HN, are monomeric and disordered in aqueous phosphate solution at pH 6.0; No correlation of solution structure with activity

scientific article published in January 2009

Allantoin and hydantoin as new protein aggregation suppressors

scientific article published on 2 March 2018

Alternative downstream processes for production of antibodies and antibody fragments

scientific article published on 21 May 2014

Amyloid fibril formation in vitro from halophilic metal binding protein: its high solubility and reversibility minimized formation of amorphous protein aggregations

scientific article published on 30 September 2013

Antiviral and Virucidal Activities of N-Cocoyl-L-Arginine Ethyl Ester ⬇️

scientific article published on November 28, 2011

Antiviral and virucidal activities of natural products

scientific article published on January 2009

Antiviral effect of arginine against herpes simplex virus type 1.

scientific article published on April 2009

Antiviral effect of octyl gallate against influenza and other RNA viruses.

scientific article published in April 2007

Antiviral effects of ascorbic and dehydroascorbic acids in vitro

scientific article

Arginine and lysine reduce the high viscosity of serum albumin solutions for pharmaceutical injection

scientific article published on 20 November 2013

Arginine as a synergistic virucidal agent

scientific article published on 08 March 2010

Arginine as an effective additive in gel permeation chromatography

scientific article published on 5 October 2005

Arginine facilitates inactivation of enveloped viruses

scientific article published on August 2008

Arginine increases the solubility of coumarin: comparison with salting-in and salting-out additives

scientific article published on 26 June 2008

Arginine inhibits adsorption of proteins on polystyrene surface

scientific article

Arginine-assisted solubilization system for drug substances: solubility experiment and simulation

scientific article published on October 2010

Biophysical characterization of a model antibody drug conjugate

scientific article published on 18 August 2016

Butyroyl-arginine as a potent virus inactivation agent

scientific article

Capto MMC mixed-mode chromatography of murine and rabbit antibodies

scientific article published on 18 July 2016

Changes in protein conformation and dynamics upon complex formation of brain-derived neurotrophic factor and its receptor: investigation by isotope-edited Fourier transform IR spectroscopy

scientific article

Channel forming outer membrane porin protein in halophile: expressed as a soluble form in Escherichia coli

scientific article published on 3 December 2012

Characterization of arginine as a solvent additive: a halophilic enzyme as a model protein

scientific article published in October 2005

Chemical and Pharmacological Chaperones: Application for Recombinant Protein Production and Protein Folding Diseases ⬇️

scientific article published on January 1, 2011

Cloning, expression, purification and activation by Na ion of halophilic alkaline phosphatase from moderate halophile Halomonas sp. 593.

scientific article published on 29 September 2010

Co-operative thermal inactivation of herpes simplex virus and influenza virus by arginine and NaCl

scientific article published on 18 September 2008

Contribution of halophilic nucleoside diphosphate kinase sequence to the heat stability of chimeric molecule

scientific article published in January 2006

Corrigendum to "Mechanism of protein desorption from 4-mercaptoethylpyridine resins by arginine solutions" [J. Chromatogr. A 1373 (2014) 141-148].

scientific article published on 13 December 2017

Cys139Ser mutation in dimeric nucleoside diphosphate kinase generates catalytically competent monomer

scientific article published on 16 February 2014

Dependence of ethanol effects on protein charges

scientific article published on 26 April 2014

Development of Calcitonin Salmon Nasal Spray: similarity of peptide formulated in chlorobutanol compared to benzalkonium chloride as preservative

scientific article

Dimeric structure of nucleoside diphosphate kinase from moderately halophilic bacterium: contrast to the tetrameric Pseudomonas counterpart

scientific article published on 15 January 2007

Distinct Characteristics of Single Starch-Binding Domain SBD1 Derived from Tandem Domains SBD1-SBD2 of Halophilic Kocuria varians Alpha-Amylase ⬇️

scientific article published on March 1, 2012

Drug solubilization effect of lauroyl-L-glutamate

scientific article published on 23 September 2011

Effect of additives on protein aggregation.

scientific article

Effect of the intermolecular disulfide bond on the conformation and stability of glial cell line-derived neurotrophic factor

scientific article published on January 2002

Effective elution of antibodies by arginine and arginine derivatives in affinity column chromatography

scientific article published in October 2005

Effects of Salt and Ligand Concentrations on the Thermal Unfolding and Refolding of Halophilic Starch-Binding Domain from Kocuria varians α-Amylase ⬇️

scientific article published on March 1, 2012

Effects of acid exposure on the conformation, stability, and aggregation of monoclonal antibodies.

scientific article

Effects of electrolytes on virus inactivation by acidic solutions ⬇️

scientific article published on April 4, 2011

Effects of mutations at Gly114 on the stability and refolding of Haloarchaeal nucleoside diphosphate kinase in low salt solution.

scientific article published on 14 February 2009

Effects of salts on protein-surface interactions: applications for column chromatography

scientific article published on July 2007

Efficient production of anti-fluorescein and anti-lysozyme as single-chain anti-body fragments (scFv) by Brevibacillus expression system

scientific article published on 20 August 2013

Electrostatic and hydrophobic interactions play a major role in the stability and refolding of halophilic proteins

scientific article published on April 2004

Elution of antibodies from a Protein-A column by aqueous arginine solutions

scientific article published in August 2004

Engineering of halophilic enzymes: two acidic amino acid residues at the carboxy-terminal region confer halophilic characteristics to Halomonas and Pseudomonas nucleoside diphosphate kinases

scientific article published on 23 June 2008

Enhanced stability of recombinant keratinocyte growth factor by mutagenesis

scientific article published on 14 February 2006

Facilitated folding and subunit assembly in Escherichia coli and in vitro of nucleoside diphosphate kinase from extremely halophilic archaeon conferred by amino-terminal extension containing hexa-His-tag

scientific article published in July 2004

Fourier transform infared spectroscopy investigation of protein conformation in spray-dried protein/trehalose powders

scientific article published in March 2004

Halophilic Properties of Metal Binding Protein Characterized by High Histidine Content from Chromohalobacter salexigens DSM3043 ⬇️

scientific article published on February 1, 2012

Halophilic beta-lactamase as a new solubility- and folding-enhancing tag protein: production of native human interleukin 1alpha and human neutrophil alpha-defensin

scientific article published on 10 November 2009

Halophilic characterization of starch-binding domain from Kocuria varians α-amylase ⬇️

scientific article published on October 15, 2011

High solubility supports efficient refolding of thermally unfolded β-lactamase ⬇️

scientific article published on September 25, 2010

Highly efficient production of VHH antibody fragments in Brevibacillus choshinensis expression system

scientific article

Highly efficient renaturation of beta-lactamase isolated from moderately halophilic bacteria

scientific article published in January 2004

Hydrophobic interaction chromatography in dual salt system increases protein binding capacity

scientific article published in August 2009

Immobilized metal affinity chromatography in the presence of arginine

scientific article published on 22 January 2009

Improved performance of column chromatography by arginine: dye-affinity chromatography

scientific article published on 25 October 2006

Inactive C8A‑humanin analog is as stable as a potent S14G‑humanin analog.

scientific article

Induced binding of proteins by ammonium sulfate in affinity and ion-exchange column chromatography

scientific article published on 15 December 2006

Influence of dietary supplementation of herb extracts on volatile sulfur production in pig large intestine

scientific article published in February 2002

Inhibition of cyclooxygenase and prostaglandin E2 synthesis by gamma-mangostin, a xanthone derivative in mangosteen, in C6 rat glioma cells

scientific article published in January 2002

Inhibitions of histamine release and prostaglandin E2 synthesis by mangosteen, a Thai medicinal plant

scientific article published in September 2002

Interaction of hexa-His tag with acidic amino acids results in facilitated refolding of halophilic nucleoside diphosphate kinase ⬇️

scientific article published on August 5, 2011

Interactions of formulation excipients with proteins in solution and in the dried state ⬇️

scientific article published on July 1, 2011

Is arginine a protein-denaturant?

scientific article

Isoform separation by a mixed-mode resin, TOYOPEARL MX-Trp-650M.

scientific article

Isoform separation of proteins by mixed-mode chromatography

scientific article published on 13 August 2015

MEP HyperCel chromatography II: binding, washing and elution

scientific article published on 12 November 2009

MEP chromatography of antibody and Fc-fusion protein using aqueous arginine solution

scientific article

Mechanisms of protein aggregation

scientific article published on June 2009

Modulation of small molecule solubility and protein binding by arginine ⬇️

scientific article published on July 20, 2010

Molecular dynamics simulation of the arginine-assisted solubilization of caffeic acid: intervention in the interaction.

scientific article

Multi-faceted arginine: mechanism of the effects of arginine on protein

scientific article published on January 2014

N-glycans stabilize human erythropoietin through hydrophobic interactions with the hydrophobic protein surface: studies by surface plasmon resonance analysis

scientific article

Non-denaturing solubilization of inclusion bodies

scientific article published in April 2010

Nondenaturing solubilization of beta2 microglobulin from inclusion bodies by L-arginine

scientific article published in March 2005

Novel soluble expression technologies derived from unique properties of halophilic proteins ⬇️

scientific article published on September 14, 2010

Novel strategy with acidic arginine solution for the treatment of influenza A virus infection ⬇️

scientific article published on March 1, 2010

Nucleoside Diphosphate Kinase from Psychrophilic Pseudoalteromonas sp. AS-131 Isolated from Antarctic Ocean

scientific article published on 5 August 2015

Opposing effects of NaCl on reversibility and thermal stability of halophilic beta-lactamase from a moderate halophile, Chromohalobacter sp. 560.

scientific article published on 26 October 2005

Practical considerations in refolding proteins from inclusion bodies

scientific article

Protein aggregation suppressor arginine as an effective mouth cleaning agent

scientific article published on 25 October 2018

Protein aggregation under high concentration/density state during chromatographic and ultrafiltration processes

scientific article published on 3 November 2016

Protein precipitation and denaturation by dimethyl sulfoxide

scientific article published on 19 September 2007

Protein solvent interaction: transition of protein-solvent interaction concenpt from basic research into solvent manupilation of chromatography

scientific article published on 24 October 2017

Protein-solvent interaction

scientific article published on 2 December 2017

Recombinant expression in moderate halophiles

scientific article published on April 2010

Recombinant production of native proteins from Escherichia coli.

scientific article

Recombinant therapeutic protein vaccines

scientific article published on December 2013

Reduction of salt-requirement of halophilic nucleoside diphosphate kinase by engineering S–S bond ⬇️

scientific article published on June 5, 2012

Residue 134 determines the dimer-tetramer assembly of nucleoside diphosphate kinase from moderately halophilic bacteria

scientific article published on 3 March 2008

Reversible Activation of Halophilic β-lactamase from Methanol-Induced Inactive Form: Contrast to Irreversible Inactivation of Non-Halophilic Counterpart

scientific article published on 20 April 2017

Reversibly bound chloride in the atrial natriuretic peptide receptor hormone-binding domain: Possible allosteric regulation and a conserved structural motif for the chloride-binding site

scientific article

Review on the application of mixed-mode chromatography for separation of structure isoforms

scientific article published on 9 October 2017

Review: Why is arginine effective in suppressing aggregation?

scientific article

Role of Us3 gene of herpes simplex virus type 1 for resistance to interferon.

scientific article published on October 2004

Role of arginine in development of biopharmaceuticals

scientific article published in June 2010

Role of arginine in protein refolding, solubilization, and purification

scientific article published in September 2004

Salt-dependent elution of uncharged aromatic solutes in ion-exchange chromatography

scientific article published on 6 March 2018

Salt-dependent thermo-reversible α-amylase: cloning and characterization of halophilic α-amylase from moderately halophilic bacterium, Kocuria varians ⬇️

scientific article published on September 25, 2010

Salts and glycine increase reversibility and decrease aggregation during thermal unfolding of ribonuclease-A.

scientific article published in April 2002

Screening of effective column rinse solvent for Protein-A chromatography

scientific article published on 6 October 2009

Secondary and quaternary structural transition of the halophilic archaeon nucleoside diphosphate kinase under high- and low-salt conditions

scientific article published in November 2002

Secretory production of single-chain antibody (scFv) in Brevibacillus choshinensis using novel fusion partner

scientific article published on 20 January 2013

Short neuroprotective peptides, ADNF9 and NAP, are structurally disordered and monomeric in PBS.

scientific article published in April 2009

Small molecule pharmacological chaperones: From thermodynamic stabilization to pharmaceutical drugs.

scientific article

Solubility enhancement of gluten and organic compounds by arginine

scientific article

Solubilization of active green fluorescent protein from insoluble particles by guanidine and arginine

scientific article

Solvent modulation of column chromatography

scientific article published on January 2008

Solvent-induced virus inactivation by acidic arginine solution.

scientific article published in March 2010

Stabilizing and destabilizing effects of arginine on deoxyribonucleic acid

scientific article published on 3 December 2009

Stress-free chromatography: IEC and HIC.

scientific article published on June 2009

Stress-free chromatography: affinity chromatography

scientific article published on June 2009

Structural characteristics of short peptides in solution

scientific article published on December 2013

Structure analysis of activity-dependent neurotrophic factor 9 by circular dichroism and sedimentation equilibrium.

scientific article published on 31 May 2007

Structure changes of natively disordered Humanin in the presence of lipid

scientific article published on 29 January 2010

Structure of Starch Binding Domains of Halophilic Alpha-Amylase at Low pH ⬇️

scientific article published on July 1, 2013

Structure of folding intermediates at pH 4.0 and native state of microbial transglutaminase.

scientific article

Structure, signaling mechanism and regulation of the natriuretic peptide receptor guanylate cyclase

scientific article

Suppression of protein interactions by arginine: a proposed mechanism of the arginine effects

scientific article published on 21 December 2006

Surface Acidic Amino Acid of Pseudomonas/Halomonas Chimeric Nucleoside Diphosphate Kinase Leads Effective Recovery from Heat- Denaturation ⬇️

scientific article published on July 1, 2013

Synergistic virus inactivation effects of arginine

scientific article published in February 2009

The biological activity of Humanin analogs correlates with structure stabilities in solution ⬇️

scientific article published on April 12, 2011

The complex structure transition of Humanin peptides by sodium dodecylsulfate and trifluoroethanol

scientific article published in January 2008

The critical role of mobile phase composition in size exclusion chromatography of protein pharmaceuticals

scientific article published on April 2010

The effects of allantoin, arginine and NaCl on thermal melting and aggregation of ribonuclease, bovine serum albumin and lysozyme

scientific article

The effects of arginine on refolding of aggregated proteins: not facilitate refolding, but suppress aggregation ⬇️

scientific article published on April 25, 2003

The effects of protein stabilizers on aggregation induced by multiple-stresses

scientific article published in November 2003

The solubility of nucleobases in aqueous arginine solutions

scientific article published on 25 March 2010

Thermal aggregation of human immunoglobulin G in arginine solutions: Contrasting effects of stabilizers and destabilizers

scientific article published on 21 June 2017

To be excluded or to bind, that is the question: Arginine effects on proteins

scientific article published on June 2009

Transition of Solvent Interaction Research from Basic Science to Applied Science

scientific article published on 19 September 2016

Trimethylamine N-oxide (TMAO) is a counteracting solute of benzyl alcohol for multi-dose formulation of immunoglobulin

scientific article

Two Elution Mechanisms of MEP Chromatography

scientific article published on 16 November 2017

Unique Features of Halophilic Proteins

scientific article published on 17 June 2016

Utilization of Arg-elution method for FLAG-tag based chromatography

scientific article published on 9 April 2009

[Familiar extremophiles, halophiles, and halophilic enzymes]

scientific article published on 01 April 2005

[Halophilic enzymes: negative charges determine halophilicity].

scientific article

gamma-Mangostin inhibits inhibitor-kappaB kinase activity and decreases lipopolysaccharide-induced cyclooxygenase-2 gene expression in C6 rat glioma cells

scientific article

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