List of works - Ina L Urbatsch

A gene optimization strategy that enhances production of fully functional P-glycoprotein in Pichia pastoris

scientific article

A stable human-cell system overexpressing cystic fibrosis transmembrane conductance regulator recombinant protein at the cell surface

scientific article published on May 2015

A survey of detergents for the purification of stable, active human cystic fibrosis transmembrane conductance regulator (CFTR).

scientific article

Asymmetry in inward- and outward-affinity constant of transport explain unidirectional lysine flux in Saccharomyces cerevisiae.

scientific article published on 23 August 2016

Bile acids stimulate ATP hydrolysis in the purified cholesterol transporter ABCG5/G8.

scientific article published on April 2010

Bradford Assay for Determining Protein Concentration

scientific article published on 01 April 2020

Combined mutation of catalytic glutamate residues in the two nucleotide binding domains of P-glycoprotein generates a conformation that binds ATP and ADP tightly

scientific article

Considerations for Membrane Protein Purification

scientific article published on 04 January 2021

Crystal structure of the human sterol transporter ABCG5/ABCG8.

scientific article published on 04 May 2016

Design and synthesis of Selenazole-containing peptides for cocrystallization with P-glycoprotein.

scientific article published on 8 March 2011

Directed evolution of P-glycoprotein cysteines reveals site-specific, non-conservative substitutions that preserve multidrug resistance.

scientific article

Distinct conformational spectrum of homologous multidrug ABC transporters

scientific article

Domain-interface dynamics of CFTR revealed by stabilizing nanobodies.

scientific article published on 14 June 2019

Erratum to: A Stable Human-Cell System Overexpressing Cystic Fibrosis Transmembrane Conductance Regulator Recombinant Protein at the Cell Surface

scientific article published on 01 May 2015

Expressing Cloned Genes for Protein Production, Purification, and Analysis

scientific article published on 03 December 2020

Expression of 25 human ABC transporters in the yeast Pichia pastoris and characterization of the purified ABCC3 ATPase activity

scientific article published on 15 June 2007

Expression of Cloned Genes Using the Baculovirus Expression System

scientific article published on 30 April 2020

Expression of Cloned Genes in Pichia pastoris Using the Methanol-Inducible Promoter AOX1

scientific article published on 04 January 2021

Expression, purification, and characterization of cysteine-free mouse P-glycoprotein

scientific article

Functional Distinction between Human and Mouse Sodium-Coupled Citrate Transporters and Its Biologic Significance: An Attempt for Structural Basis Using a Homology Modeling Approach

scientific article published on 11 October 2020

Functional analysis of a species-specific inhibitor selective for human Na+-coupled citrate transporter (NaCT/SLC13A5/mINDY)

scientific article published on 20 October 2020

Functional reconstitution of human ABCC3 into proteoliposomes reveals a transport mechanism with positive cooperativity

scientific article

Interactions and cooperativity between P-glycoprotein structural domains determined by thermal unfolding provides insights into its solution structure and function

scientific article published on 23 October 2016

Membrane protein stability can be compromised by detergent interactions with the extramembranous soluble domains

scientific article published on 3 May 2014

Methods for Measuring the Concentrations of Proteins

scientific article published on 01 April 2020

Mutational analysis of conserved aromatic residues in the A-loop of the ABC transporter ABCB1A (mouse Mdr3)

scientific article published on 03 January 2007

Nucleotide binding to the human multidrug resistance protein 3, MRP3.

scientific article published in July 2010

Nucleotide binding to the multidrug resistance P-glycoprotein as studied by ESR spectroscopy

scientific article

Nucleotide dependence of the dimerization of ATP binding cassette nucleotide binding domains.

scientific article published on 17 October 2016

Nucleotide-induced structural changes in P-glycoprotein observed by electron microscopy.

scientific article

P-glycoprotein catalytic mechanism: studies of the ADP-vanadate inhibited state

scientific article published in April 2003

P-glycoprotein is fully active after multiple tryptophan substitutions ⬇️

scientific article published on December 19, 2012

Preparation of Cell Extracts for Purification of Proteins Expressed in Pichia pastoris

scientific article published on 04 January 2021

Projection structure of P-glycoprotein by electron microscopy. Evidence for a closed conformation of the nucleotide binding domains

scientific article

Properties of P-glycoprotein with mutations in the "catalytic carboxylate" glutamate residues

scientific article

Purification and ATP hydrolysis of the putative cholesterol transporters ABCG5 and ABCG8

scientific article

Purification of Fusion Proteins by Affinity Chromatography on Glutathione Resin

scientific article published on 01 June 2020

Purification of Polyhistidine-Tagged Proteins by Immobilized Metal Affinity Chromatography

scientific article published on 01 June 2020

Replacing the eleven native tryptophans by directed evolution produces an active P-glycoprotein with site-specific, non-conservative substitutions

scientific article published on 21 February 2020

Snapshots of ligand entry, malleable binding and induced helical movement in P-glycoprotein

scientific article

Specific lipids modulate the transporter associated with antigen processing (TAP).

scientific article

Specific stabilization of CFTR by phosphatidylserine

scientific article published on 29 November 2016

Spina Bifida

Structural stability of purified human CFTR is systematically improved by mutations in nucleotide binding domain 1.

scientific article published on 6 February 2018

Structure of P-glycoprotein reveals a molecular basis for poly-specific drug binding

scientific article

Structures of P-glycoprotein reveal its conformational flexibility and an epitope on the nucleotide-binding domain

scientific article

Substrate-induced conformational changes in the nucleotide-binding domains of lipid bilayer-associated P-glycoprotein during ATP hydrolysis

scientific article

Understanding polyspecificity of multidrug ABC transporters: closing in on the gaps in ABCB1

scientific article

List of works by Ina L Urbatsch

A gene optimization strategy that enhances production of fully functional P-glycoprotein in Pichia pastoris

A stable human-cell system overexpressing cystic fibrosis transmembrane conductance regulator recombinant protein at the cell surface

A survey of detergents for the purification of stable, active human cystic fibrosis transmembrane conductance regulator (CFTR).

Asymmetry in inward- and outward-affinity constant of transport explain unidirectional lysine flux in Saccharomyces cerevisiae.

Bile acids stimulate ATP hydrolysis in the purified cholesterol transporter ABCG5/G8.

Bradford Assay for Determining Protein Concentration

Combined mutation of catalytic glutamate residues in the two nucleotide binding domains of P-glycoprotein generates a conformation that binds ATP and ADP tightly

Considerations for Membrane Protein Purification

Crystal structure of the human sterol transporter ABCG5/ABCG8.

Design and synthesis of Selenazole-containing peptides for cocrystallization with P-glycoprotein.

Directed evolution of P-glycoprotein cysteines reveals site-specific, non-conservative substitutions that preserve multidrug resistance.

Distinct conformational spectrum of homologous multidrug ABC transporters

Domain-interface dynamics of CFTR revealed by stabilizing nanobodies.

Erratum to: A Stable Human-Cell System Overexpressing Cystic Fibrosis Transmembrane Conductance Regulator Recombinant Protein at the Cell Surface

Expressing Cloned Genes for Protein Production, Purification, and Analysis

Expression of 25 human ABC transporters in the yeast Pichia pastoris and characterization of the purified ABCC3 ATPase activity

Expression of Cloned Genes Using the Baculovirus Expression System

Expression of Cloned Genes in <i>Pichia pastoris</i> Using the Methanol-Inducible Promoter <i>AOX1</i>

Expression, purification, and characterization of cysteine-free mouse P-glycoprotein

Functional Distinction between Human and Mouse Sodium-Coupled Citrate Transporters and Its Biologic Significance: An Attempt for Structural Basis Using a Homology Modeling Approach

Functional analysis of a species-specific inhibitor selective for human Na+-coupled citrate transporter (NaCT/SLC13A5/mINDY)

Functional reconstitution of human ABCC3 into proteoliposomes reveals a transport mechanism with positive cooperativity

Interactions and cooperativity between P-glycoprotein structural domains determined by thermal unfolding provides insights into its solution structure and function

Membrane protein stability can be compromised by detergent interactions with the extramembranous soluble domains

Methods for Measuring the Concentrations of Proteins

Mutational analysis of conserved aromatic residues in the A-loop of the ABC transporter ABCB1A (mouse Mdr3)

Nucleotide binding to the human multidrug resistance protein 3, MRP3.

Nucleotide binding to the multidrug resistance P-glycoprotein as studied by ESR spectroscopy

Nucleotide dependence of the dimerization of ATP binding cassette nucleotide binding domains.

Nucleotide-induced structural changes in P-glycoprotein observed by electron microscopy.

P-glycoprotein catalytic mechanism: studies of the ADP-vanadate inhibited state

P-glycoprotein is fully active after multiple tryptophan substitutions ⬇️

Preparation of Cell Extracts for Purification of Proteins Expressed in <i>Pichia pastoris</i>

Projection structure of P-glycoprotein by electron microscopy. Evidence for a closed conformation of the nucleotide binding domains

Properties of P-glycoprotein with mutations in the "catalytic carboxylate" glutamate residues

Purification and ATP hydrolysis of the putative cholesterol transporters ABCG5 and ABCG8

Purification of Fusion Proteins by Affinity Chromatography on Glutathione Resin

Purification of Polyhistidine-Tagged Proteins by Immobilized Metal Affinity Chromatography

Replacing the eleven native tryptophans by directed evolution produces an active P-glycoprotein with site-specific, non-conservative substitutions

Snapshots of ligand entry, malleable binding and induced helical movement in P-glycoprotein

Specific lipids modulate the transporter associated with antigen processing (TAP).

Specific stabilization of CFTR by phosphatidylserine

Spina Bifida

Structural stability of purified human CFTR is systematically improved by mutations in nucleotide binding domain 1.

Structure of P-glycoprotein reveals a molecular basis for poly-specific drug binding

Structures of P-glycoprotein reveal its conformational flexibility and an epitope on the nucleotide-binding domain

Substrate-induced conformational changes in the nucleotide-binding domains of lipid bilayer-associated P-glycoprotein during ATP hydrolysis

Understanding polyspecificity of multidrug ABC transporters: closing in on the gaps in ABCB1