List of works by Stephen P Bottomley

A biophysical analysis of the tetratricopeptide repeat-rich mitochondrial import receptor, Tom70, reveals an elongated monomer that is inherently flexible, unstable, and unfolds via a multistate pathway

scientific article

A common fold mediates vertebrate defense and bacterial attack

scientific article

A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production

scientific article

A method for the incremental expansion of polyglutamine repeats in recombinant proteins

scientific article published in January 2013

A protein family under 'stress' - serpin stability, folding and misfolding

scientific article

A structural basis for loop C-sheet polymerization in serpins

scientific article

Acid Denaturation of alpha1-antitrypsin: characterization of a novel mechanism of serpin polymerization.

scientific article published in December 2002

Aeropin from the extremophile Pyrobaculum aerophilum bypasses the serpin misfolding trap

scientific article

Antagonism of antiviral and allogeneic activity of a human public CTL clonotype by a single altered peptide ligand: implications for allograft rejection

scientific article

Antigen ligation triggers a conformational change within the constant domain of the alphabeta T cell receptor

scientific article published on 21 May 2009

Antithrombin: in control of coagulation

scientific article published on March 2004

Cleaved antitrypsin polymers at atomic resolution

scientific article

Conformational change in the chromatin remodelling protein MENT

scientific article

Conformational properties of the disease-causing Z variant of α1-antitrypsin revealed by theory and experiment

scientific article

Crystal structure of a UDP-glucose-specific glycosyltransferase from a Mycobacterium species

scientific article

Crystallization and X-ray analysis of a single fab binding domain from protein L of Peptostreptococcus magnus

scientific article published on 01 December 1995

DNA accelerates the inhibition of human cathepsin V by serpins

scientific article

Destabilization of a non-pathological variant of ataxin-3 results in fibrillogenesis via a partially folded intermediate: a model for misfolding in polyglutamine disease.

scientific article

Determination of the P1', P2' and P3' subsite-specificity of factor Xa.

scientific article

Different conformational changes within the F-helix occur during serpin folding, polymerization, and proteinase inhibition

scientific article

Discovery of amino acid motifs for thrombin cleavage and validation using a model substrate

scientific article

Dissection of Protease-Activated Receptor-1-Dependent and -Independent Responses to Thrombin in Skeletal Myoblasts

article

Enhanced molecular mobility of ordinarily structured regions drives polyglutamine disease

scientific article

Enhancing the stability and solubility of TEV protease using in silico design

scientific article

Evidence that serpin architecture intrinsically supports papain-like cysteine protease inhibition: engineering alpha(1)-antitrypsin to inhibit cathepsin proteases

scientific article

Expression and purification of the human thrombin receptor

scientific article published on June 1998

Expression, purification and characterization of recombinant Z alpha(1)-antitrypsin--the most common cause of alpha(1)-antitrypsin deficiency.

scientific article published on 23 June 2009

Flanking domain stability modulates the aggregation kinetics of a polyglutamine disease protein

scientific article published on 18 August 2011

Functional insights from the distribution and role of homopeptide repeat-containing proteins

scientific article

Generation and expression of a minimal hybrid Ig-receptor formed between single domains from proteins L and G.

scientific article published on 22 November 2007

Human embryonic stem cell models of Huntington disease.

scientific article

Identification and Characterization of a Misfolded Monomeric Serpin Formed at Physiological Temperature ⬇️

scientific article published on September 17, 2010

Identification of residual structure within denatured antichymotrypsin: implications for serpin folding and misfolding

scientific article published in November 2004

In vitro and in silico design of alpha1-antitrypsin mutants with different conformational stabilities

scientific article

Inhibitory activity of a heterochromatin-associated serpin (MENT) against papain-like cysteine proteinases affects chromatin structure and blocks cell proliferation

scientific article

Intracellular Production of Recombinant Serpins in Yeast

article

Introduction of a mutation in the shutter region of antithrombin (Phe77 --> Leu) increases affinity for heparin and decreases thermal stability

scientific article published in September 2003

Kinetic instability of the serpin Z alpha1-antitrypsin promotes aggregation.

scientific article published on 26 November 2009

Mechanisms of Ataxin-3 Misfolding and Fibril Formation: Kinetic Analysis of a Disease-associated Polyglutamine Protein

article

Methods to Measure the Kinetics of Protease Inhibition by Serpins

article

Molecular determinants of the mechanism underlying acceleration of the interaction between antithrombin and factor Xa by heparin pentasaccharide

scientific article published on 19 February 2002

Molecular gymnastics: serpin structure, folding and misfolding

scientific article

Natural HLA class I polymorphism controls the pathway of antigen presentation and susceptibility to viral evasion

scientific article

Nucleocytoplasmic coagulation: an injury-induced aggregation event that disulfide crosslinks proteins and facilitates their removal by plasmin

scientific article

Oxidation of an exposed methionine instigates the aggregation of glyceraldehyde-3-phosphate dehydrogenase.

scientific article

PFD: a database for the investigation of protein folding kinetics and stability

scientific article

Physical characterization of serpin conformations

scientific article

Physicochemical properties that control protein aggregation also determine whether a protein is retained or released from necrotic cells.

scientific article

PolyQ: a database describing the sequence and domain context of polyglutamine repeats in proteins ⬇️

scientific article published on November 8, 2010

Polyglutamine expansion in ataxin-3 does not affect protein stability: implications for misfolding and disease.

scientific article published in September 2004

Preventing serpin aggregation: The molecular mechanism of citrate action upon antitrypsin unfolding

scientific article

Probing the folding pathway of a consensus serpin using single tryptophan mutants

scientific article published in February 2018

Probing the role of the F-helix in serpin stability through a single tryptophan substitution

scientific article

Probing the unfolding pathway of alpha1-antitrypsin.

scientific article published in April 1999

Production of recombinant serpins in Escherichia coli.

scientific article

Promiscuous beta-strand interactions and the conformational diseases.

scientific article published on February 2004

Protease-activated receptor-2 mediates proliferative responses in skeletal myoblasts

scientific article published on 01 December 2000

Protein expression and refolding--a practical guide to getting the most out of inclusion bodies

scientific article published in January 2004

Protein unfolding is essential for cleavage within the α-helix of a model protein substrate by the serine protease, thrombin.

scientific article

Purification of polyglutamine proteins.

scientific article

REFOLD: an analytical database of protein refolding methods

scientific article published on 15 August 2005

Recombinant caspase-3 expressed in Pichia pastoris is fully activated and kinetically indistinguishable from the native enzyme.

scientific article published in September 1997

Recombinant protein quality evaluation: proposal for a minimal information standard

scientific article (publication date: 30 November 2011)

Refolding Your Protein with a Little Help from REFOLD

scientific article published on 01 January 2011

Serpin acceleration of amyloid fibril formation: a role for accessory proteins

scientific article published on 22 November 2006

Serpin polymerization and its role in disease--the molecular basis of alpha1-antitrypsin deficiency

scientific article published on January 2009

Serpins 2005 - fun between the beta-sheets. Meeting report based upon presentations made at the 4th International Symposium on Serpin Structure, Function and Biology (Cairns, Australia).

scientific article published in October 2005

Serpins flex their muscle. I. PUTTING THE CLAMPS ON PROTEOLYSIS IN DIVERSE BIOLOGICAL SYSTEMS.

scientific article published in December 2010

Serpins flex their muscle: I. Putting the clamps on proteolysis in diverse biological systems

scientific article

Serpins flex their muscle: II. Structural insights into target peptidase recognition, polymerization, and transport functions

scientific article

Serpins in Prokaryotes

Serpins in the Caenorhabditis elegans genome

scientific article published on 01 July 1999

Serpins in theCaenorhabditis elegans genome

scholarly article by James C. Whisstock published in July 1999

Serpins' mystery solved

scientific article published in Nature

Serpins: finely balanced conformational traps

scientific article

Small heat-shock proteins interact with a flanking domain to suppress polyglutamine aggregation.

scientific article

Smoothing a rugged protein folding landscape by sequence-based redesign

scientific article published on 26 September 2016

Structural and biochemical characterization of the oxidoreductase NmDsbA3 from Neisseria meningitidis

scientific article

Structural and functional analysis of the Josephin domain of the polyglutamine protein ataxin-3

article

Structural change in β-sheet A of Z α(1)-antitrypsin is responsible for accelerated polymerization and disease

scientific article

Structure of a serpin-enzyme complex probed by cysteine substitutions and fluorescence spectroscopy

scientific article

The 1.5 A crystal structure of a prokaryote serpin: controlling conformational change in a heated environment

scientific article (publication date: April 2003)

The 1.5 Å Crystal Structure of a Prokaryote Serpin

article

The N terminus of the serpin, tengpin, functions to trap the metastable native state

scientific article

The REFOLD database: a tool for the optimization of protein expression and refolding

scientific article

The Rate of PolyQ-Mediated Aggregation Is Dramatically Affected by the Number and Location of Surrounding Domains

scientific article published on 16 September 2011

The Structure of the Bacterial Oxidoreductase Enzyme DsbA in Complex with a Peptide Reveals a Basis for Substrate Specificity in the Catalytic Cycle of DsbA Enzymes

scientific article

The Subtilisin-Like Protease AprV2 Is Required for Virulence and Uses a Novel Disulphide-Tethered Exosite to Bind Substrates

scientific article

The Z mutation alters the global structural dynamics of α1-antitrypsin

scientific article

The high resolution crystal structure of a native thermostable serpin reveals the complex mechanism underpinning the stressed to relaxed transition

scientific article

The high resolution crystal structure of the human tumor suppressor maspin reveals a novel conformational switch in the G-helix

scientific article

The human serpin proteinase inhibitor-9 self-associates at physiological temperatures

scientific article published on July 2004

The inositol polyphosphate 5-phosphatases and the apurinic/apyrimidinic base excision repair endonucleases share a common mechanism for catalysis

scientific article

The loss of tryptophan 194 in antichymotrypsin lowers the kinetic barrier to misfolding

scientific article published on 03 July 2007

The matrix refolded

article

The murine orthologue of human antichymotrypsin: a structural paradigm for clade A3 serpins

scientific article

The role of protein misfolding in the pathogenesis of human diseases.

scientific article published on March 2004

The role of strand 1 of the C beta-sheet in the structure and function of alpha(1)-antitrypsin

scientific article

The roles of helix I and strand 5A in the folding, function and misfolding of α1-antitrypsin

scientific article

The selective inhibition of serpin aggregation by the molecular chaperone, alpha-crystallin, indicates a nucleation-dependent specificity

scientific article published on 18 September 2003

The structural impact of a polyglutamine tract is location-dependent

scientific article published on 10 October 2008

The structure of H-2K(b) and K(bm8) complexed to a herpes simplex virus determinant: evidence for a conformational switch that governs T cell repertoire selection and viral resistance

scientific article published in July 2004

The two-stage pathway of ataxin-3 fibrillogenesis involves a polyglutamine-independent step.

scientific article

Thrombin, a Survival Factor for Cultured Myoblasts

scientific article published in Journal of Biological Chemistry

Tracking the unfolding pathway of a multirepeat protein via tryptophan scanning: evidence of localized instability in the mitochondrial import receptor Tom70.

scientific article published on 27 June 2006

X-ray crystal structure of MENT: evidence for functional loop-sheet polymers in chromatin condensation

scientific article

α1-Antitrypsin Polymerisation Can Occur by both Loop A and C Sheet Mechanisms

scientific article published on 01 October 1998

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