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List of works by David A. Holowka

A basic sequence in STIM1 promotes Ca2+ influx by interacting with the C-terminal acidic coiled coil of Orai1.

scientific article published on February 2010

A guanine nucleotide-binding protein participates in IgE receptor-mediated activation of endogenous and reconstituted phospholipase A2 in a permeabilized cell system

scientific article published on 01 January 1990

A lipid raft environment enhances Lyn kinase activity by protecting the active site tyrosine from dephosphorylation

scientific journal article

A nanosecond fluorescence depolarization study on the segmental flexibility of receptor-bound immunoglobulin E

scientific article published on 01 May 1990

A novel fluorescence-based biosynthetic trafficking method provides pharmacologic evidence that PI4-kinase IIIα is important for protein trafficking from the endoplasmic reticulum to the plasma membrane

scientific article

Activated Cdc42/Rac reconstitutes Fcepsilon RI-mediated Ca2+ mobilization and degranulation in mutant RBL mast cells

scientific article

Activation of Cdc42 is necessary for sustained oscillations of Ca2+ and PIP2 stimulated by antigen in RBL mast cells

scientific article

Aggregation of IgE-receptor complexes on rat basophilic leukemia cells does not change the intrinsic affinity but can alter the kinetics of the ligand-IgE interaction

scientific article published on June 16, 1992

Alteration of lipid composition modulates Fc epsilon RI signaling in RBL-2H3 cells

scientific article published on April 1995

Altered patterns of tyrosine phosphorylation and Syk activation for sterically restricted cyclic dimers of IgE-Fc epsilonRI.

scientific article published on February 1997

An Interaction Library for the FcεRI Signaling Network

scientific article

Antigen-decorated shell cross-linked nanoparticles: synthesis, characterization, and antibody interactions

scientific article

Antigen-mediated IGE receptor aggregation and signaling: a window on cell surface structure and dynamics

scientific article published on January 1996

Antigen-stimulated trafficking from the recycling compartment to the plasma membrane in RBL mast cells

scientific article

Antigen-triggered membrane potential changes in IgE-sensitized rat basophilic leukemia cells: evidence for a repolarizing response that is important in the stimulation of cellular degranulation

scientific article published in January 1989

Association of the receptor for immunoglobulin E with an endogenous polypeptide on rat basophilic leukemia cells

scientific article published on 01 January 1980

Basic Amino Acids Within the Juxtamembrane Domain of the Epidermal Growth Factor Receptor Regulate Receptor Dimerization and Auto-phosphorylation

scientific article published on 19 November 2020

Beyond Media Composition: Cell Plasma Membrane Disruptions by Graphene Oxide

scientific article published on 01 March 2017

Binding mechanisms of PEGylated ligands reveal multiple effects of the PEG scaffold

scientific article published on 23 December 2007

Bivalent binding of an anti-CD3 antibody to Jurkat cells induces association of the T cell receptor complex with the cytoskeleton

scientific article published on 01 August 1989

Bivalent ligand dissociation kinetics from receptor-bound immunoglobulin E: evidence for a time-dependent increase in ligand rebinding at the cell surface ⬇️

scientific article published on March 5, 1991

Bivalent ligands with rigid double-stranded DNA spacers reveal structural constraints on signaling by Fc epsilon RI.

scientific article published in July 2002

Bringing light to ER contacts and a new phase in organelle communication

scientific article published on 28 April 2020

Ca2+ waves initiate antigen-stimulated Ca2+ responses in mast cells

scientific article

Cancer cell-derived microvesicles induce transformation by transferring tissue transglutaminase and fibronectin to recipient cells

scientific article

Characterization of increased K+ permeability associated with the stimulation of receptors for immunoglobulin E on rat basophilic leukemia cells ⬇️

scientific article published on August 15, 1991

Characterization of model antigens composed of biotinylated haptens bound to avidin

scientific article published on 01 February 1990

Chemical modification and fluorescence studies of chloroplast coupling factor ⬇️

scientific article published on December 13, 1977

Cholera toxin increases the rate of antigen-stimulated calcium influx in rat basophilic leukemia cells

scientific article published on 01 December 1988

Clustering, mobility, and triggering activity of small oligomers of immunoglobulin E on rat basophilic leukemia cells

scientific article

Coexisting domains in the plasma membranes of live cells characterized by spin-label ESR spectroscopy

scientific article

Compartmentalized activation of the high affinity immunoglobulin E receptor within membrane domains

scientific article published on February 1997

Competition between solution and cell surface receptors for ligand. Dissociation of hapten bound to surface antibody in the presence of solution antibody

scientific article

Computation of a Theoretical Membrane Phase Diagram and the Role of Phase in Lipid-Raft-Mediated Protein Organization

scientific article published on 12 February 2018

Conformation of immunoglobulin M. 2. Nanosecond fluorescence depolarization analysis of segmental flexibility in anti-epsilon-l-dimethylamino-5-naphthalenesulfonyl-L-lysine anti-immunoglobulin from horse, pig, and shark

scientific article published on July 27, 1976

Conformation of immunoglobulin M. I. Characterization of anti-epsilon-1-dimethylamino-5-naphthalenesulfonyl-L-lysine immunoglobulin M antibodies from horse, pig, and shark

scientific article (publication date: 27 July 1976)

Conformations of IgE bound to its receptor Fc epsilon RI and in solution

scientific article published on 01 September 1991

Correlation functions quantify super-resolution images and estimate apparent clustering due to over-counting

scientific article

Critical fluctuations in plasma membrane vesicles.

scientific article

Critical role for cholesterol in Lyn-mediated tyrosine phosphorylation of FcepsilonRI and their association with detergent-resistant membranes

scientific article

Cross-correlation analysis of inner-leaflet-anchored green fluorescent protein co-redistributed with IgE receptors and outer leaflet lipid raft components

scientific article

Cross-linking of IgE-receptor complexes at the cell surface: a fluorescence method for studying the binding of monovalent and bivalent haptens to IgE.

scientific article published on July 1986

Cross-linking of IgE-receptor complexes at the cell surface: synthesis and characterization of a long bivalent hapten that is capable of triggering mast cells and rat basophilic leukemia cells

scientific article published on 01 July 1986

Cross-linking of IgE-receptor complexes by rigid bivalent antigens greater than 200 A in length triggers cellular degranulation

scientific article

Cross-linking of immunoglobulin E-receptor complexes induces their interaction with the cytoskeleton of rat basophilic leukemia cells

scientific article published in June 1986

Cross-linking of receptor-bound IgE to aggregates larger than dimers leads to rapid immobilization

scientific article

Crosslinking a lipid raft component triggers liquid ordered-liquid disordered phase separation in model plasma membranes

scientific article

Development of a mast cell-based biosensor

scientific article published on 24 October 2007

Differential targeting of secretory lysosomes and recycling endosomes in mast cells revealed by patterned antigen arrays.

scientific article published on 14 August 2007

Disruption of lipid order by short-chain ceramides correlates with inhibition of phospholipase D and downstream signaling by FcepsilonRI.

scientific article

Dissociation kinetics of bivalent ligand-immunoglobulin E aggregates in solution

scientific article published in March 1991

Distinct stages of stimulated FcεRI receptor clustering and immobilization are identified through superresolution imaging.

scientific article

Dynamic conformations compared for IgE and IgG1 in solution and bound to receptors

scientific article published on August 25, 1992

Effects of subunit mutation on the rotational dynamics of Fc epsilon RI, the high affinity receptor for IgE, in transfected cells

scientific article published on May 1995

Electron spin resonance characterization of liquid ordered phase of detergent-resistant membranes from RBL-2H3 cells

scientific article

Evidence supporting a role for microfilaments in regulating the coupling between poorly dissociable IgE-Fc epsilonRI aggregates downstream signaling pathways

scientific article published on June 1997

Fc epsilon RI and the T cell receptor for antigen activate similar signalling pathways in T cell-RBL cell hybrids

scientific article published on March 1993

Fc epsilon RI-mediated association of 6-micron beads with RBL-2H3 mast cells results in exclusion of signaling proteins from the forming phagosome and abrogation of normal downstream signaling

scientific article

Fc epsilon RI-mediated recruitment of p53/56lyn to detergent-resistant membrane domains accompanies cellular signaling

scientific article published on 26 September 1995

Fc(epsilon)RI as a paradigm for a lipid raft-dependent receptor in hematopoietic cells

scientific article

FcεRI Signaling in Specialized Membrane Domains

Fluorescence anisotropy measurements of lipid order in plasma membranes and lipid rafts from RBL-2H3 mast cells

scientific article

Fluorescence resonance energy transfer between lipid probes detects nanoscopic heterogeneity in the plasma membrane of live cells

scientific article published on 26 February 2007

Fluorescence resonance energy transfer reveals interleukin (IL)-1-dependent aggregation of IL-1 type I receptors that correlates with receptor activation

scientific article published on 01 November 1995

Focal adhesion proteins connect IgE receptors to the cytoskeleton as revealed by micropatterned ligand arrays

scientific article

Functional nanoscale coupling of Lyn kinase with IgE-FcεRI is restricted by the actin cytoskeleton in early antigen-stimulated signaling

scientific article published on 28 September 2016

Functionalized surface arrays for spatial targeting of immune cell signaling

scientific article published in May 2006

Further characterization of the beta-component of the receptor for immunoglobulin E.

scientific article

Graphene Oxide Nanosheets Stimulate Ruffling and Shedding of Mammalian Cell Plasma Membranes.

scientific article published on August 2016

Heterogeneous Glycosylation of Immunoglobulin E Constructs Characterized by Top-Down High-Resolution 2-D Mass Spectrometry†

scientific article published on 01 March 2000

Heterologous desensitization of the high affinity receptor for IgE (Fc epsilon R1) on RBL cells

scientific article published on May 1993

Highly Effective Poly(Ethylene Glycol) Architectures for Specific Inhibition of Immune Receptor Activation ⬇️

scientific article published on November 11, 2003

How does the plasma membrane participate in cellular signaling by receptors for immunoglobulin E?

scientific article

IgE receptor-mediated alteration of membrane-cytoskeleton interactions revealed by mass spectrometric analysis of detergent-resistant membranes

scientific article published on July 2009

Imaging FCS delineates subtle heterogeneity in plasma membranes of resting mast cells

scientific article published on 02 January 2020

In situ measurement of degranulation as a biosensor based on RBL-2H3 mast cells.

scientific article

Inhibitors of PI(4,5)P2 synthesis reveal dynamic regulation of IgE receptor signaling by phosphoinositides in RBL mast cells.

scientific article published on 11 January 2013

Insights into immunoglobulin E receptor signaling from structurally defined ligands

scientific article published on June 2007

Interaction of IgE with its high-affinity receptor. Structural basis and requirements for effective cross-linking.

scientific article

Interaction of photoreactive and fluorescent nucleotides with chloroplast coupling factor 1 ⬇️

scientific article published on August 7, 1979

Interactions between Fc(epsilon)RI and lipid raft components are regulated by the actin cytoskeleton.

scientific article

Interleukin 1 (IL-1) causes changes in lateral and rotational mobilities of IL-1 type I receptors

scientific article published on 01 February 1999

Kinetics of multivalent antigen DNP-BSA binding to IgE-Fc epsilon RI in relationship to the stimulated tyrosine phosphorylation of Fc epsilon RI ⬇️

scientific article published on 01 April 1998

Lactoperoxidase-catalyzed iodination of the receptor for immunoglobulin E at the cytoplasmic side of the plasma membrane ⬇️

scientific article published on March 25, 1984

Large-scale co-aggregation of fluorescent lipid probes with cell surface proteins

scientific article

Large-scale fluid/fluid phase separation of proteins and lipids in giant plasma membrane vesicles

scholarly article

Lateral Diffusion of Membrane Lipid-Anchored Probes before and after Aggregation of Cell Surface IgE-Receptors†

Lipid rafts, fluid/fluid phase separation, and their relevance to plasma membrane structure and function

scientific article

Lipid segregation and IgE receptor signaling: a decade of progress

scientific article

Mapping the site of interaction between murine IgE and its high affinity receptor with chimeric Ig

scientific article published on 01 December 1990

Mast Cell Activation on Patterned Lipid Bilayers of Subcellular Dimensions†

Mechanisms of epidermal growth factor receptor signaling as characterized by patterned ligand activation and mutational analysis

scientific article published on 23 December 2016

Membrane organization in immunoglobulin E receptor signaling

scientific article

Membrane-bound IgE receptor complexes fused with rat basophilic leukemia cells mediate degranulation

scientific article

Microfilaments regulate the rate of exocytosis in rat basophilic leukemia cells

scientific article published in August 1990

Micropatterned Ligand Arrays to Study Spatial Regulation in Fc Receptor Signaling ⬇️

scientific article published on January 1, 2011

Molecular anatomy of the early events in STIM1 activation - oligomerization or conformational change?

scientific article

Molecular clustering of STIM1 with Orai1/CRACM1 at the plasma membrane depends dynamically on depletion of Ca2+ stores and on electrostatic interactions.

scientific article

Molecular crowding on the cell surface

scientific article published on 01 January 1988

Molecular mechanisms of spontaneous and directed mast cell motility

scientific article

Mutant RBL mast cells defective in Fc epsilon RI signaling and lipid raft biosynthesis are reconstituted by activated Rho-family GTPases

scientific article

Mutations in the polybasic juxtamembrane sequence of both plasma membrane- and endoplasmic reticulum-localized epidermal growth factor receptors confer ligand-independent cell transformation

scientific article published on 18 October 2013

Nanobiotechnology and cell biology: micro- and nanofabricated surfaces to investigate receptor-mediated signaling

scientific article published on January 2008

Nanodomains in early and later phases of FcɛRI signalling

scientific article

Near-field fluorescence cross-correlation spectroscopy on planar membranes.

scientific article

Non-Faradaic Electrochemical Detection of Exocytosis from Mast and Chromaffin Cells Using Floating-Gate MOS Transistors

scientific article

Ordered and disordered phases coexist in plasma membrane vesicles of RBL-2H3 mast cells. An ESR study

scientific article

Polyunsaturated fatty acids inhibit stimulated coupling between the ER Ca(2+) sensor STIM1 and the Ca(2+) channel protein Orai1 in a process that correlates with inhibition of stimulated STIM1 oligomerization.

scientific article

Proteolytic digestion of the beta and gamma subunits of the receptor for immunoglobulin E at the cytoplasmic face of the plasma membrane

scientific article published in January 1989

Quantitative Analysis of Phospholipids in Functionally Important Membrane Domains from RBL-2H3 Mast Cells Using Tandem High-Resolution Mass Spectrometry†

article

Quantitative nanoscale analysis of IgE-FcεRI clustering and coupling to early signaling proteins.

scientific article published on 2 April 2012

Rab11 Regulates the Mast Cell Exocytic Response

scientific article published on 11 June 2016

Real-time cross-correlation image analysis of early events in IgE receptor signaling

scientific journal article

Real-time imaging of Ca(2+) mobilization and degranulation in mast cells

scientific article

Recent evidence for common signalling mechanisms among immunoreceptors that recognize foreign antigens

scientific article

Recombinant human IgG1-murine IgE chimeric Ig. Construction, expression, and binding to human Fc gamma receptors

scientific article published on 01 December 1990

Reconstitution of regulated phosphorylation of FcepsilonRI by a lipid raft-excluded protein-tyrosine phosphatase

scientific article published on 10 November 2004

Regulation of exocytosis and mitochondrial relocalization by Alpha-synuclein in a mammalian cell model

scientific article published on 27 June 2019

Roles for Ca2+ Mobilization and Its Regulation in Mast Cell Functions ⬇️

scientific article published on May 8, 2012

Roles for Ca2+ mobilization and its regulation in mast cell functions: recent progress

scientific article published on April 2016

Roles for SH2 and SH3 domains in Lyn kinase association with activated FcepsilonRI in RBL mast cells revealed by patterned surface analysis

scientific article

Roles for lipid heterogeneity in immunoreceptor signaling

scientific article published on 16 March 2016

Rotational motion of monomeric and dimeric immunoglobulin E-receptor complexes ⬇️

scientific article published on January 21, 1992

STIM1 activation is regulated by a 14 amino acid sequence adjacent to the CRAC activation domain.

scientific article published on 28 February 2016

Segmental flexibility of receptor-bound immunoglobulin E

scientific article published on 01 December 1985

Sequestration of phosphoinositides by mutated MARCKS effector domain inhibits stimulated Ca2+mobilization and degranulation in mast cells ⬇️

scientific article published on October 19, 2011

Short chain ceramides disrupt immunoreceptor signaling by inhibiting segregation of Lo from Ld Plasma membrane components

scientific article published on 27 September 2018

Simultaneous cross-linking by two nontriggering bivalent ligands causes synergistic signaling of IgE Fc epsilon RI complexes.

scientific article

Small oligomers of immunoglobulin E (IgE) cause large-scale clustering of IgE receptors on the surface of rat basophilic leukemia cells ⬇️

scientific article published on February 1, 1984

Spatially defined EGF receptor activation reveals an F-actin-dependent phospho-Erk signaling complex.

scientific article

Spatiotemporal resolution of mast cell granule exocytosis reveals correlation with Ca2+ wave initiation

scientific article

Sphingosine derivatives inhibit cell signaling by electrostatically neutralizing polyphosphoinositides at the plasma membrane ⬇️

scientific article published on January 1, 2010

Stimulated association of STIM1 and Orai1 is regulated by the balance of PtdIns(4,5)P2 between distinct membrane pools ⬇️

scientific article published on July 12, 2011

Stimulated release of fluorescently labeled IgE fragments that efficiently accumulate in secretory granules after endocytosis in RBL-2H3 mast cells ⬇️

scientific article published on August 1, 1998

Structural analyses of the receptor for IgE on rat basophilic leukemia cells

scientific article published on 01 January 1981

Structural aspects of the association of FcepsilonRI with detergent-resistant membranes

scientific article published on January 1999

Structural determinants for partitioning of lipids and proteins between coexisting fluid phases in giant plasma membrane vesicles

scientific article published on 12 September 2007

Structural mapping of Fc receptor bound immunoglobulin E: proximity to the membrane surface of the antibody combining site and another site in the Fab segments

scientific article published on October 1985

Structural mapping of membrane-bound immunoglobulin E-receptor complexes: use of monoclonal anti-IgE antibodies to probe the conformation of receptor-bound IgE

scientific article published on 01 October 1985

Structural studies on the membrane-bound immunoglobulin E-receptor complex. 1. Characterization of large plasma membrane vesicles from rat basophilic leukemia cells and insertion of amphipathic fluorescent probes ⬇️

scientific article published on July 5, 1983

Structure of the high-affinity mast cell receptor for IgE.

scientific article published on January 1982

Sustained T cell receptor-mediated Ca2+ responses rely on dynamic engagement of receptors

scientific article published in May 1996

Temporally resolved interactions between antigen-stimulated IgE receptors and Lyn kinase on living cells.

scientific article

The Fc segment of IgE influences the kinetics of dissociation of a symmetrical bivalent ligand from cyclic dimeric complexes

scientific article published on 01 April 1996

The FcεRI Signaling Cascade and Integrin Trafficking Converge at Patterned Ligand Surfaces.

scientific article published on 9 August 2017

The beta- and gamma-isoforms of type I PIP5K regulate distinct stages of Ca2+ signaling in mast cells

scientific article

The effect of receptor density on the forward rate constant for binding of ligands to cell surface receptors

scientific article

Timescale Separation of Positive and Negative Signaling Creates History-Dependent Responses to IgE Receptor Stimulation

scientific article published on 14 November 2017

Toxoplasma gondii inhibits mast cell degranulation by suppressing phospholipase Cγ-mediated Ca2+ mobilization ⬇️

scientific article published on July 4, 2013

Transmembrane sequences are determinants of immunoreceptor signaling

scientific article

Trivalent ligands with rigid DNA spacers reveal structural requirements for IgE receptor signaling in RBL mast cells

scientific article published on October 2007

Visualization of plasma membrane compartmentalization with patterned lipid bilayers.

scholarly article

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