List of works - Mitsi A Blount

A 46-amino acid segment in phosphodiesterase-5 GAF-B domain provides for high vardenafil potency over sildenafil and tadalafil and is involved in phosphodiesterase-5 dimerization.

scientific article

A timely characterization of vasopressin-sensitive adenylyl cyclase isoforms in the mouse inner medullary collecting duct

scientific article published on 23 December 2009

Absence of PKC-alpha attenuates lithium-induced nephrogenic diabetes insipidus

scientific article

Activation of protein kinase Cα increases phosphorylation of the UT-A1 urea transporter at serine 494 in the inner medullary collecting duct

scientific article

Acute calcineurin inhibition with tacrolimus increases phosphorylated UT-A1.

scientific article

Allosteric sites of phosphodiesterase-5 sequester cyclic GMP.

scientific article published in January 2004

Binding of tritiated sildenafil, tadalafil, or vardenafil to the phosphodiesterase-5 catalytic site displays potency, specificity, heterogeneity, and cGMP stimulation

scientific article

Candesartan augments compensatory changes in medullary transport proteins in the diabetic rat kidney

scientific article published on 16 April 2008

Chronic lithium treatment induces novel patterns of pendrin localization and expression

scientific article published on 18 April 2018

Chronic use of chloroquine disrupts the urine concentration mechanism by lowering cAMP levels in the inner medulla ⬇️

scientific article published on July 11, 2012

Collecting duct-specific knockout of adenylyl cyclase type VI causes a urinary concentration defect in mice.

scientific article published on 21 September 2011

Conversion of phosphodiesterase-5 (PDE5) catalytic site to higher affinity by PDE5 inhibitors

scientific article published on 09 August 2007

Epac regulates UT-A1 to increase urea transport in inner medullary collecting ducts.

scientific article published on 06 August 2009

Expression of transporters involved in urine concentration recovers differently after cessation of lithium treatment

scientific article

Forskolin stimulates phosphorylation and membrane accumulation of UT-A3.

scientific article

Genes and proteins of urea transporters

scientific article

High lung PDE5: a strong basis for treating pulmonary hypertension with PDE5 inhibitors

scientific article published in September 2005

Lack of an effect of collecting duct-specific deletion of adenylyl cyclase 3 on renal Na+ and water excretion or arterial pressure.

scientific article published on 15 January 2014

Lack of protein kinase C-α leads to impaired urine concentrating ability and decreased aquaporin-2 in angiotensin II-induced hypertension ⬇️

scientific article published on April 4, 2012

Lack of urea transporters, UT-A1 and UT-A3, increases nitric oxide accumulation to dampen medullary sodium reabsorption through ENaC

scientific article published on 12 December 2018

Mammalian Cyclic Nucleotide Phosphodiesterases: Molecular Mechanisms and Physiological Functions ⬇️

scientific article published on April 1, 2011

Metal ion stimulators of PDE5 cause similar conformational changes in the enzyme as does cGMP or sildenafil ⬇️

scientific article published on December 25, 2010

Metformin, an AMPK activator, stimulates the phosphorylation of aquaporin 2 and urea transporter A1 in inner medullary collecting ducts.

scientific article published on 09 March 2016

Molecular mechanisms of urea transport in health and disease ⬇️

scientific article published on September 25, 2012

Molecular properties of mammalian proteins that interact with cGMP: protein kinases, cation channels, phosphodiesterases, and multi-drug anion transporters

scientific article

Novel activators of aquaporin 2 membrane expression for the treatment of nephrogenic diabetes insipidus: less is more. Focus on "High-throughput chemical screening identifies AG-490 as a stimulator of aquaporin 2 membrane expression and urine concen

scientific article published on 18 June 2014

Phosphorylation increases affinity of the phosphodiesterase-5 catalytic site for tadalafil

scientific article

Phosphorylation of UT-A1 on serine 486 correlates with membrane accumulation and urea transport activity in both rat IMCDs and cultured cells

scientific article

Phosphorylation of UT-A1 urea transporter at serines 486 and 499 is important for vasopressin-regulated activity and membrane accumulation

scientific article published on 21 May 2008

Phosphorylation of phosphodiesterase-5 is promoted by a conformational change induced by sildenafil, vardenafil, or tadalafil

scientific article published on 01 January 2007

Purinergic signaling is enhanced in the absence of UT-A1 and UT-A3

scientific article published on 01 January 2021

Radiolabeled ligand binding to the catalytic or allosteric sites of PDE5 and PDE11.

scientific article published in January 2005

Regulation of renal urea transport by vasopressin

scientific article

Smooth Muscle-Targeted Overexpression of Peroxisome Proliferator Activated Receptor-γ Disrupts Vascular Wall Structure and Function

scientific article

The role of nitric oxide in the dysregulation of the urine concentration mechanism in diabetes mellitus

scientific article published on 6 June 2012

Transgenic Restoration of Urea Transporter A1 Confers Maximal Urinary Concentration in the Absence of Urea Transporter A3.

scientific article published on 25 September 2015

Urea Transport in the Kidney ⬇️

scientific article published on April 1, 2011

Urea transporters UT-A1 and UT-A3 accumulate in the plasma membrane in response to increased hypertonicity

scientific article published on 20 August 2008

Urine concentration in the diabetic mouse requires both urea and water transporters

scientific article published on 7 November 2012

Vardenafil: structural basis for higher potency over sildenafil in inhibiting cGMP-specific phosphodiesterase-5 (PDE5).

scientific article

Vasopressin increases plasma membrane accumulation of urea transporter UT-A1 in rat inner medullary collecting ducts

scientific article published on 07 September 2006

Vasopressin regulation of multisite phosphorylation of UT-A1 in the inner medullary collecting duct

scientific article

[3H]sildenafil binding to phosphodiesterase-5 is specific, kinetically heterogeneous, and stimulated by cGMP.

scientific article published in June 2003

miR-23a is decreased during muscle atrophy by a mechanism that includes calcineurin signaling and exosome-mediated export

scientific article published on 11 December 2013

List of works by Mitsi A Blount

A 46-amino acid segment in phosphodiesterase-5 GAF-B domain provides for high vardenafil potency over sildenafil and tadalafil and is involved in phosphodiesterase-5 dimerization.

A timely characterization of vasopressin-sensitive adenylyl cyclase isoforms in the mouse inner medullary collecting duct

Absence of PKC-alpha attenuates lithium-induced nephrogenic diabetes insipidus

Activation of protein kinase Cα increases phosphorylation of the UT-A1 urea transporter at serine 494 in the inner medullary collecting duct

Acute calcineurin inhibition with tacrolimus increases phosphorylated UT-A1.

Allosteric sites of phosphodiesterase-5 sequester cyclic GMP.

Binding of tritiated sildenafil, tadalafil, or vardenafil to the phosphodiesterase-5 catalytic site displays potency, specificity, heterogeneity, and cGMP stimulation

Candesartan augments compensatory changes in medullary transport proteins in the diabetic rat kidney

Chronic lithium treatment induces novel patterns of pendrin localization and expression

Chronic use of chloroquine disrupts the urine concentration mechanism by lowering cAMP levels in the inner medulla ⬇️

Collecting duct-specific knockout of adenylyl cyclase type VI causes a urinary concentration defect in mice.

Conversion of phosphodiesterase-5 (PDE5) catalytic site to higher affinity by PDE5 inhibitors

Epac regulates UT-A1 to increase urea transport in inner medullary collecting ducts.

Expression of transporters involved in urine concentration recovers differently after cessation of lithium treatment

Forskolin stimulates phosphorylation and membrane accumulation of UT-A3.

Genes and proteins of urea transporters

High lung PDE5: a strong basis for treating pulmonary hypertension with PDE5 inhibitors

Lack of an effect of collecting duct-specific deletion of adenylyl cyclase 3 on renal Na+ and water excretion or arterial pressure.

Lack of protein kinase C-α leads to impaired urine concentrating ability and decreased aquaporin-2 in angiotensin II-induced hypertension ⬇️

Lack of urea transporters, UT-A1 and UT-A3, increases nitric oxide accumulation to dampen medullary sodium reabsorption through ENaC

Mammalian Cyclic Nucleotide Phosphodiesterases: Molecular Mechanisms and Physiological Functions ⬇️

Metal ion stimulators of PDE5 cause similar conformational changes in the enzyme as does cGMP or sildenafil ⬇️

Metformin, an AMPK activator, stimulates the phosphorylation of aquaporin 2 and urea transporter A1 in inner medullary collecting ducts.

Molecular mechanisms of urea transport in health and disease ⬇️

Molecular properties of mammalian proteins that interact with cGMP: protein kinases, cation channels, phosphodiesterases, and multi-drug anion transporters

Novel activators of aquaporin 2 membrane expression for the treatment of nephrogenic diabetes insipidus: less is more. Focus on "High-throughput chemical screening identifies AG-490 as a stimulator of aquaporin 2 membrane expression and urine concen

Phosphorylation increases affinity of the phosphodiesterase-5 catalytic site for tadalafil

Phosphorylation of UT-A1 on serine 486 correlates with membrane accumulation and urea transport activity in both rat IMCDs and cultured cells

Phosphorylation of UT-A1 urea transporter at serines 486 and 499 is important for vasopressin-regulated activity and membrane accumulation

Phosphorylation of phosphodiesterase-5 is promoted by a conformational change induced by sildenafil, vardenafil, or tadalafil

Purinergic signaling is enhanced in the absence of UT-A1 and UT-A3

Radiolabeled ligand binding to the catalytic or allosteric sites of PDE5 and PDE11.

Regulation of renal urea transport by vasopressin

Smooth Muscle-Targeted Overexpression of Peroxisome Proliferator Activated Receptor-γ Disrupts Vascular Wall Structure and Function

The role of nitric oxide in the dysregulation of the urine concentration mechanism in diabetes mellitus

Transgenic Restoration of Urea Transporter A1 Confers Maximal Urinary Concentration in the Absence of Urea Transporter A3.

Urea Transport in the Kidney ⬇️

Urea transporters UT-A1 and UT-A3 accumulate in the plasma membrane in response to increased hypertonicity

Urine concentration in the diabetic mouse requires both urea and water transporters

Vardenafil: structural basis for higher potency over sildenafil in inhibiting cGMP-specific phosphodiesterase-5 (PDE5).

Vasopressin increases plasma membrane accumulation of urea transporter UT-A1 in rat inner medullary collecting ducts

Vasopressin regulation of multisite phosphorylation of UT-A1 in the inner medullary collecting duct

[3H]sildenafil binding to phosphodiesterase-5 is specific, kinetically heterogeneous, and stimulated by cGMP.

miR-23a is decreased during muscle atrophy by a mechanism that includes calcineurin signaling and exosome-mediated export