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List of works by Giedrius Gasiunas

5' modifications to CRISPR-Cas9 gRNA can change the dynamics and size of R-loops and inhibit DNA cleavage

scientific article published on 04 June 2020

A catalogue of biochemically diverse CRISPR-Cas9 orthologs

scientific article published on 02 November 2020

Applications of the Versatile CRISPR-Cas Systems

Applications of the Versatile CRISPR-Cas Systems

Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/Cas immune system

scientific article

Cas3 nuclease-helicase activity assays

scientific article published in January 2015

Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria ⬇️

scientific article (publication date: 25 September 2012)

Design of a CRISPR-Cas system to increase resistance of Bacillus subtilis to bacteriophage SPP1.

scientific article published on 2 June 2016

Direct observation of R-loop formation by single RNA-guided Cas9 and Cascade effector complexes.

scientific article

DnaQ exonuclease-like domain of Cas2 promotes spacer integration in a type I-E CRISPR-Cas system

scientific article published on 11 June 2018

Harnessing the natural diversity and in vitro evolution of Cas9 to expand the genome editing toolbox

scientific article

In vitro reconstitution of Cascade-mediated CRISPR immunity in Streptococcus thermophilus.

scientific article published on 18 January 2013

Methods for decoding Cas9 protospacer adjacent motif (PAM) sequences: A brief overview

scientific article published on 23 March 2017

Molecular mechanisms of CRISPR-mediated microbial immunity

scientific article

Mva1269I: a monomeric type IIS restriction endonuclease from Micrococcus varians with two EcoRI- and FokI-like catalytic domains

scientific article

Programmable DNA cleavage in vitro by Cas9.

scientific article published on December 2013

RNA-dependent DNA endonuclease Cas9 of the CRISPR system: Holy Grail of genome editing?

scientific article

Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements

scientific article published on 19 November 2015

Rewiring Cas9 to Target New PAM Sequences

scientific article published in March 2016

Streptococcus thermophilus CRISPR-Cas9 Systems Enable Specific Editing of the Human Genome

scientific article published on 14 December 2015

Structure of the DNA-Bound Spacer Capture Complex of a Type II CRISPR-Cas System

scientific article published on 09 May 2019

Targeted gene editing by transfection of in vitro reconstituted Streptococcus thermophilus Cas9 nuclease complex

scientific article published on January 2015

Tetrameric restriction enzymes: expansion to the GIY-YIG nuclease family

scientific article published on 17 December 2007

The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli

scientific article

crRNA and tracrRNA guide Cas9-mediated DNA interference in Streptococcus thermophilus

scientific article published on 27 March 2013

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